9 2 2 0 3 4 South China Fisheries Science Vol. 9, No. 2 Apr., 203 doi: 0. 3969/ j. issn. 2095-0780. 203. 02. 02, 2,,,, (., 48000; 2., 4028) : 25 g ( Monopterus albus) 2 400, 5,, 4 0 gkg - 20kg g - 30kg g - 40 gkg - ( Vitis amurensis) ( Artemisia annua) ( 4 ), 2 28 00 d, ( GOT) ( GPT) ( TP), ( GLU) ( TG), 20 gkg - 30 gkg - ( P < 0. 05) ; 0 g kg - 40 gkg - ( P > 0. 05) 20kg g - 30 gkg - ( P > 0. 05), 20 30 g kg -, : ; ; ; ; : S 963. 73; S 966. 4 : A : 2095-0780 - ( 203) 02-0070 - 06 Effects of plant extract from grape seed and sweet wormwood on intestinal digestive enzyme activities and blood biochemical parameters of eel ( Monopterus albus) HUANG Guangzhong, HU Hui, XIAO Keyu 2, LUO Shimin, XIE Peirong, OUYANG Juying (. Huaihua Vocational and Technical College, Huaihua 48000, China; 2. Hunan Agricultural University, Changsha 4028, China) Abstract: To explore the growth promotion mechanism of plant extract from grape ( Vitis amurensis) seed and sweet wormwood on Monopterus albus, 2 400 healthy eel fingerlings were randomly divided into five groups. We fed with basal feed, and four experimental groups with diets containing respectively 0 gkg -, 20 gkg -, 30 gkg -, 40 g kg - mixture, which were extracted from grape seed and from sweet wormwood( Artemisia annua) with a four-to-one proportion. We determined the digestive enzyme activities and blood biochemical parameters of five eel groups after feeding for 00 d in 2 28. Results show that basal diets with the mixture could improve digestive enzyme, blood glutamic-oxaloacetic transaminase ( GOT) and glutamic-pyruvic transaminase ( GPT) s activities, and triglyceride ( TG) s contents of eel. Compared with the, and total protein ( TP) s content; and could reduce blood glucose ( GLU) the above indexes had significant difference ( P < 0. 05) when the dosage of the plant extract was 20 gkg - or 30 gkg -, while the above indexes had no significant difference ( P > 0. 05) when it was 0 gkg - or 40 gkg -. There was no significant difference between 20 gkg - and 30 gkg - experimental groups. In conclusion, extract from grape : 202-07-7; : 202- -29 : ( 202NK3056) : ( 967 - ),,,, E-mail: hzhgzh@ 26. com
2 : 7 seed and sweet wormwood with a four-to-one ratio, when mixed in diets with dosage 20 30 gkg -, could increase digestive enzyme activities, regulate blood biochemical parameters, enhance the digestion and metabolic activity of 3 main nutritive materials and improve the growth of fish. Key words: plant extract; grape seed; Monopterus albus; digestive enzyme; blood biochemical parameter, ( ) ;, (, ], ) 2], 3], 4], 5] 6] 7] 8 ]. 4, ( Vitis amuren- sis) ( Artemisia annua), 90%, 20 30, cm 7 d 00 gm - 2,, ; ( Monopterus albus), 30 gl - 3 5 min,, 2 ) : 3 d, 3 d,,,. 2) : ( 25 0. 6) g 2 400, 5. 2,. 6 m;, 5 :, 9: 00, 4 6, 30 2 28, ph 6. 8 7. 5 30, 2. 0 m. 5. 5 m2. 0 m,. 5., 8 0 g kg - 20 kg g - 30kg g - 40 gkg -, 2. 5 ml, ( ), ( 2), 4 8 h, 5 000 rmin - 0 min, ( ) - 80. 3 ;, 2,,, - 80, 24 h 4, 20 5 20 8 23 00 d 8: 00 Tab. Experimental design / gkg - experimental group / g - initial weight 25. 0 0. 29 24. 93 0. 64 25. 00 0. 57 25. 3 0. 24 24. 30 0. 4 - / number 80 80 80 80 80 / gkg - dosage of extract 0
72 9 ingredient 2 Tab. 2 Ingredients and nutritional level in basal diet w/ % content nutritional level w/ % content soybean meal 34. 0 / MJ kg - total energy 4. 48 inferior flour 6. 0 crude protein 34. 26 rapeseed meal 4. 0 crude lipid 3. 85 corn protein powder 5. 0 ash 6. 24 wheat bran 5. 0 calcium 0. 78 Peruvian fish meal 0. 0 total phosphorus. 2 cottonseed meal 8. 0 available phosphorus 0. 8 rapeseed oil 3. 0 lysine. 89 talc. 4 glutamic acid 5. 5 calcium dihydrogen phosphate. 5 adhesive 0. 5 choline 0. 3 salt 0. 3 premix. 0 : : B 0 mgkg -, B 2 20 mg kg -, B 6 30 mgkg -, B 2 0. 02 mgkg -, 5 mgkg -, 50 mgkg -, 00 mgkg -, 00 mg kg -, K 0 mg kg -, D 2 000 IUkg -, kg -, 60 mgkg -,. 0 mgkg -, 0. 3 mg kg - 0. 5 mgkg -, C 00 mgkg -, A 8 000 IU kg -, E 00 mgkg -, 250 mgkg -, 80 mg kg -, 2 mgkg -, 20 mg Note: remix ingredient: vitamine B 0 mgkg -, vitamine B 2 20 mg kg -, vitamine B 6 30 mgkg -, vitamine B 2 0. 02 mgkg -, folic acid 5 mg kg -, calcium pantothenate 50 mgkg -, myoinositol 00 mgkg -, niacin 00 mgkg -, biltin 0. 5 mgkg -, vitamine C 00 mg kg -, vitamine A 8 000 IUkg -, vitamine K 0 mgkg -, vitamine D 2 000 IUkg -, vitamine E 00 mgkg -, magnesium 250 mg kg -, iron 80 mg kg -, copper 2 mgkg -, manganese 20 mgkg -, zinc 60 mgkg -, iodine. 0 mg kg -, selenium 0. 3 mg kg -.. 5. 2 ),, -,, : = ( W t - W 0 ) / W 0 00; = ( lnw t - lnw 0 ) / t] 00; = F/ ( W t + W d - W 0 ) ; = ( W t - W 0 ) /( F P) 00; = / ; ( TG) TCH) 00, W t ( g) ; W 0 ( g) ; W d ( g) ; P. 6 ( % ) ; F ( g) ; t ( d). 5. 3 ( X SD),, = 9 0. 86%, 2. 2 000 rmin - 0 min, (. 5. 4 ( GOT) ( GPT), ( TP), ; ( GLU) -, SPSS 7. 0, Duncan s, P C 0. 05 2 00 d
2 : 73 ( 3), 20 gkg - 30 g kg - ( P < 0. 05), 0 gkg - ( 4) 3, 40 gkg - ( P > 0. 05) 20 gkg - 30 gkg -,, 20 gkg - 30 gkg - 00% 33. 33%, ( P < 0. 05), 0 g kg - 40 gkg - ( P 97. 08% 69. 20%, 70% > 0. 05), ( P > 0. 05) 4 ( P > 0. 05) kg -, 2. 2 ( P > 0. 05), 75. 95%, ( P < 0. 05) ; 40 g ( P > 0. 05) 20kg g - 30 gkg - 3 Tab. 3 Effect of plant extract on growth performance of M. albus / gkg - experimental group /g - first weight 25. 0 0. 29 24. 93 0. 64 25. 00 0. 57 25. 3 0. 24 25. 20 0. 4 /g - last weight 65. 59 0. 65 a 68. 67 0. 95 ab 74. 2 0. b 73. 5 0. 95 b 69. 8 0. 69 ab /% weight gain rate 62. 26 6. 23 a 75. 45 8. 5 ab 96. 48. 07 b 92. 52 0. 58 b 77. 02 8. 62 ab /% protein efficiency ratio 02. 2 4. 05 a 0. 27 5. 79 ab 9. 84 6. 9 b 20. 98 5. 35 b 0. 37 4. 65 ab /% d - specific growth rate 0. 42 0. 0 a 0. 44 0. 02 ab 0. 47 0. 02 b 0. 47 0. 02 b 0. 44 0. 0 ab feed coefficient 2. 93 0. a 2. 88 0. 3 ab 2. 75 0. 09 b 2. 73 0. 7 b 2. 87 0. 20 ab /% survival ratio 97. 50 96. 25 97. 50 00. 00 98. 75 : ( P >0. 05), ( P C 0. 05), Note: alues with same superscripts within the same row have no significant difference( P > 0. 05 ), and those with different superscripts were significantly different from one another( P < 0. 05 ). The same case in the following tables. 4 Tab. 4 Effect of plant extract on digestive enzyme activities of M. albus / g kg - experimental group / U. mg - amylase 0. 03 0. 00 a 0. 04 0. 0 a 0. 06 0. 0 b 0. 07 0. 0 b 0. 05 0. 0 ab / U. g - lipase 2. 76 0. 20 a 3. 33 0. 8 a 22. 85. 0 b 2. 59 0. 96 b 6. 6 0. 79 ab / U. mg - trypsin 94. 00 0. 53 a 05. 89 2. 40 a 59. 80 8. 45 b 65. 39 22. 55 b 00. 3 9. 9 a 2. 3 GOTGPT TCH, TP,, GLU 3. 76% 38. 0%, ( 5), GOT 20 g kg - 30 gkg - GLU 37. 57% 30. 0% 68. 4%, 30 g kg - GOT 38. 0%, ( P < 0. 05), 0 g kg - 40 kg g - ( 68. 4% ), ( P < 0. 05) ; GPT 3. 63% 68. 79%, 30 g kg - GPT 28. 98%, 20 g kg - 30 gkg - TG ( 68. 79% ), ( P < 0. 05) ; TP 7. 5% 4. 4%, 20 gkg - TP 4. 4%, ( P < 0. 05), GLUTG ( P > 0. 05) ; TG. 8% 28. 35% 28. 98%, ( P < 0. 05), ( P >0. 05) ; TCH 3. 73% 7. 4%, ( P >0. 05)
74 9 5 Tab. 5 Effect of plant extract on blood biochemical parameters of M. albus / gkg - experimental group / IUL - GOT 406. 2 0. 23 a 528. 0 35. 08 ab 638. 8 4. 79 b 682. 84 0. 69 b 588. 23 75. 97 b / IUL - GPT 26. 96 7. 00 a 67. 2 3. 05 ab 24. 29 9. 55 b 22. 53 43. 6 b 79. 98 2. 63 ab ( ) / gl - TP 37. 94 0. 9 a 40. 79 3. 64 a 53. 65 6. 47 b 45. 7. 52 ab 42. 30 5. 08 a c( ) / mmoll - GLU 3. 78 0. 05 a 3. 26 0. 08 ab 2. 36 0. 06 b 2. 34 0. 9 b 2. 85 0. ab c( ) / mmoll - TG 6. 35 0. 9 a 5. 64 0. 2 ab 4. 55 0. 07 b 4. 5 0. 3 b 5. 35 0. 08 ab c( ) / mmoll - TCH 4. 02 0. 55 3. 87 0. 08 3. 66 0. 3 3. 58 0. 20 3. 67 0. 4 3 3.,, ( 3), 6] ( 5), 4,, 20 g kg - 30 g kg - GOTGPT TP 68. 4% 68. 79% 4. 4%,, GOTGPT TP 3. 2, 20 gkg - GPTTP, 30 gkg - GOT, 40 gkg - GOT GPT TP 9] ( 4),,, 4 20 30 gkg - 33. 33% GLU 75. 95% 97. 08%,, 7 ], 8],,, GLU,, 30 gkg - ( 38. 0% ), 20 g kg - 30 gkg -, 40 gkg - 20 gkg - 30 gkg -, 30 gkg -, 0] TG ] 2],, TCH, 9] 20],, TCHTG 20 30 gkg -, 20 gkg - 30 g kg - TG, 28. 35% 28. 98%,, 3 ], TGTCH, 6. 53%, TG, TCH, 3. 3, ; GPTGOTTPGLUTG TCH,,, 4] GPTGOT TP 5] TP, GOT GPT, 20], TGTCH GOT GPT 2],,
2 : 75 TCH, J]., 0],,,. J]., 2005, 25( 6) : 29-3., ],,,.,, GPT J]., 2008, 27( 9) : 490-493. GOTTP, GLUTGTCH, 2],,,. 5, J]., 2007, 35 ( 26 ) : 20 30 8236-8239. gkg - 3],,,. : ],,. J]., 2009, J]., 200 ( 5 ) : 43-45. 2],,,., 200, 28( ) : 34-37. J]., 2008, 20 ( 5 ) : 547-553. 3],,. 4( 5) : 743-748. J]., 2006, 30( ) : 90-96. 4],. J]. :, 2002, 4 J]., 2002, 22( 4) : 42-43. 5],,,. 4 8]. J]. J]., 2008, 27( 2 ) : 67-70. 6],,,. 8 9],,,. 5 J]., 2008, 28( 5) : 2-4. 7],,. 20],. J]., 2006 ( ) : 37-39. 8],,,. 9 2],,,. J]., 200 ( 0 ) : 865-868. 9],,,. EA-II BA-I, 993, 8( ) : 79-82. J]., 200, 3( 4) : 4-44. 4],,,. - 39( 3) : 46-5. 5],. J]. 6],,,. J]., 2007, 7],,,. ( 3) : 64-67., 2008, ( 6) : 288-289. J]., 2003, 35 ( ) : 8-0. J]., 2003, 4 ( 3 ) : 68-70. J]., 202, 8 ( 2) : 58-63.