Education Alessandra L. Ferzoco 26 Washington Ave. Cambridge, Ma 02140 (919) 357-6648 ferzoco@rowland.harvard.edu www. Ph.D. Physical Chemistry, 2012 University of North Carolina at Chapel Hill Research Advisor - Gary L. Glish Dissertation: Understanding MS Characterization of Peptide Structures B.S. Chemistry, 2004 College of Creative Studies, University of California at Santa Barbara Research Advisors - Michael T. Bowers and Cynthia Husted Research Appointments Rowland Fellow The Rowland Institute at Harvard University, 2011 to 2016 Development of a cryogenic ion trap for gas phase fluorescence spectroscopy Flow through electrochemistry for the analysis of reaction intermediates by mass spectrometry A liquid phase quadrupole for preparative mass spectrometry Doctoral Research Assistant Department of Chemistry, The University of North Carolina at Chapel Hill, 2005 to 2011, Advisor: Gary L. Glish The study of gas phase peptide fragment structures and dissociation mechanisms The study of IR energy deposition as a function of analyte size Derivatization of peptides to improve de novo sequencing Contributed to instrumentation projects including: a temperature controlled quadrupole ion trap for infrared spectroscopy; a differential ion mobility device for in-source shape selection; an electrospray/pyrolysis ionization source for the study of cigarette smoke Undergraduate Research Assistant Department of Chemistry and Biochemistry, University of California at Santa Barbara, 2003-2004, Advisor: Michael T. Bowers Ion mobility mass spectrometry for the study of helix formation in gas phase oligonucleotides Undergraduate Research Assistant Neuroscience Research Institute, University of California at Santa Barbara, 1998-2002 (began work in high school), Advisor: Cynthia Husted High performance liquid chromatography and Langmuir-Blodgett force microscopy for the study of lipids in the myelin membranes of fish, rats, and marmosets infected with a multiple sclerosis disease model Maintenance of a medicinal plant greenhouse for the study of therapeutic properties in neurodegenerative diseases
Professional Service and Outreach Activities President: Greater Boston Mass Spectrometry Discussion Group, 2015 to Present Poster Judge: Annual Conference on Mass Spectrometry and Allied Topics, 2016 to Present Education Committee Member: American Society for Mass Spectrometry, 2015 to 2017 Meal server at Rosie s Place (women s shelter), 2013 to present Chair and co-chair: Fundamentals Interest Group of the American Society for Mass Spectrometry, 2014-2016 Coordinator: Local Mass Spectrometry Discussion Group Consortium organized through the American Society for Mass Spectrometry 2014 and 2015 Volunteer: Greater Boston Mass Spectrometry Discussion Group, 2013 to 2015 Session chair: Fundamentals: Ion spectroscopy, 60 th Annual Conference on Mass Spectrometry and Allied Topics, June 2012 Journal review: Journal of Physical Chemistry, Accounts of Chemical Research, Journal of the American Society for Mass Spectrometry Proposal review: National Science Foundation The above represents scientific service, and volunteer activities I am currently involved in. I would happily provide a more extensive list of volunteer and outreach activities upon request. Teaching Experience Graduate (University of North Carolina at Chapel Hill) Instructor, Physical Chemistry I: Thermodynamics (Chem481), Fall 2010 Teaching assistant, Electronics Lab (Chem441L), Fall 2009 GAANN Elementary and Jr. High after school program physics group leader, Fall 2007 Spring 2009 Teaching assistant and guest lecturer with Gary L. Glish, Mass Spectrometry (Chem442), Fall 2008 Guest lecturer with John Papanikolas, Physical Chemistry I: Thermodynamics (Chem481), Spring 2008 Head teaching assistant, Physical Chemistry Lab I (Chem481L), Fall 2006 Teaching assistant, General Chemistry Lab II (Chem102L), Fall 2005 Spring 2006 Undergraduate (University of California at Santa Barbara) Teaching assistant, Cell Biology Lab (MCDB103L), Winter 2004 Teaching assistant, Cell Biology (MCDB103), Winter 2003 Accepted Proposals Determination of reaction intermediates in the methyl viologen and 4,4 -bipyridinium catalyzed reduction of carbon dioxide, granted by the FELIX Facility at Radbound University, August 2016
From light energy to chemical energy: A gas phase spectroscopy instrument designed to study proton coupled electron transfer mechanisms, granted by the Rowland Institute at Harvard, March 2011 Dependence of a4 structure on glycine position, granted by the FOM Institute for Plasma Physics Rijnhuizen, February 2010 Why do peptide b3 ions react differently than other bn ions? granted by the FOM Institute for Plasma Physics Rijnhuizen, June 2009 Awards and Honors Graduate 2011 American Society for Mass Spectrometry Sanibel travel award 2007-2008 Graduate Research Consultant for undergraduate research at the University of North Carolina 2007 University of North Carolina Future Faculty Fellowship 2006-2009 Graduate Assistance in Areas of National Need (GAANN) fellowship 2006 University of North Carolina Department of Chemistry Annual Teaching Award 2005 Francis P. Venable fellowship Undergraduate Research internships in science and engineering (RISE) fellowship Summer undergraduate research fellowship (SURF) Publications and Presentations * Indicates publications as corresponding author Refereed Publications Rajagopal, V.; Stokes, C.; Ferzoco, A. L.*; A cryogenic linear ion trap designed for gas phase fluorescence spectroscopy, J. Am. Soc. Mass Spectrom. 2017, DOI: 10.1007/s13361-017-1763-3. Gidden, J.; Baker, E. S.; Ferzoco, A. L.; Bowers, M. T. Structural motifs of DNA complexes in the gas phase, Int. J. Mass Spectrom. 2005, 240, 183-193. Gidden, J.; Ferzoco, A. L.; Baker, E. S; Bowers, M. T. Duplex formation and the onset of helicity in poly d(cg)n oligonucleotides in a solvent free environment, J. Am. Chem. Soc. 2004, 126, 15132-15140. Baker, E. S.; Gidden, J.; Ferzoco, A. L.; Bowers, M. T. Sodium stabilization of dinucleotide multiplexes in the gas phase, Phys. Chem. Chem. Phys. 2004, 6, 2786-2795. In preparation Ferzoco, A. L.; Martens, J.; Berden, G.; Oomens, J.; Glish, G. L. C terminal Sequencing of Peptides using a Sodium Cationized FMOC Derivatization in preparation for J. Am. Soc. Mass Spectrom.
Ferzoco, A. L.; Crizer, D.; Isenberg, S. L; Steill, J.; Maitre, P.; Oomens, J.; Leary, J.; Glish, G. L.; Observation of oxazolone and diketopiperazine structures in b3 product ions from leucine enkephalin derivatives, in preparation for J. Phys. Chem. A Ferzoco, A. L.; Glish, G. L. Efficient dissociation of large peptides with IRMPD in preparation for J. Am. Soc. Mass Spectrom. Ferzoco, A. L.; Steill, J.; Oomens, J.; Glish, G. L. Structural similarity in a4 ions despite different dissociation pathways, in preparation for J. Phys. Chem. A Patents Ko, Y. J.; Ferzoco, A. L.* Quadrupole Particle Filter, U.S. Patent Application No. 61/936,787, filed February 6, 2015. Book Chapters Wyttenbach, T.; Baker, E. S.; Berenstein, S. L.; Ferzoco, A. L.; Gidden, J.; Liu, D.; Bowers, M. T. Ion Mobility mass spectrometry method and its application to duplex formation of oligonucleotides and aggregation of proteins, In: Advances in Mass Spectrometry, A. E. Ashcroft, G. Brenton, J. J. Monaghan (eds.), Amsterdam, Elsevier, 2004, Vol. 16, pp. 189-200. Invited Presentations Oral Presentation: Rajagopal, V.; Stokes, C.; Ferzoco, A. L. A cryogenic linear ion trap with expanded electrode spacing designed for fluorescence spectroscopy of excited state charge transfer complexes PHYS division at the 2017 ACS Fall meeting in Washington D.C., August 2017 Departmental Seminar: Gas phase fluorescence spectroscopy to understand energy transport mechanisms, Bowdoin College, Department of Chemistry, November 2, 2016 Oral presentation: Ferzoco, A. L.; Ridgeway, M. E.; Kaplan, D. A.; Park, M. A.; Glish, G. L. FAIMS as a Filter for Biomarker Detection, American Chemical Society Fall 2010 National Meeting and Exposition, August 2010. Conference Presentations (contributed as first and/or corresponding author) Poster presentation: Rajagopal, V.; Ferzoco, A. L. A Cryogenic Linear Ion Trap for Fluorescence Spectroscopy, 65 th ASMS Conference on Mass Spectrometry and Allied Topics, June 2017 Poster presentation: Rajagopal, V.; Ferzoco, A. L. A Cryogenic Linear Ion Trap for Fluorescence Spectroscopy, Gordon Research Conference on Gaseous Ions: Structures, Energetics & Reactions, February 2017 Highlight talk and poser presentation: Rajagopal, V.; Ferzoco, A. L. A Linear Ion Trap Designed for Gas Phase Fluorescence Spectroscopy of Chromophore-Solvent Complexes, ASMS Asilomar Conference on Novel Instrumentation, October 2016 Poster presentation: Rajagopal, V.; Ferzoco, A. L. Gas Phase Fluorescence Spectroscopy for the Study of Catalytic Nitrogen-Containing Heterocycles, American Chemical Society Fall 2016 National Meeting and Exposition, August 2016
Poster presentation: Ferzoco, A. L. Instrument development that connects mass spectrometry to the solution phase: spectroscopy of solvated complexes, flowthrough electrochemistry, and preparative scale mass spectrometry, American Chemical Society Fall 2016 National Meeting and Exposition, August 2016 Poster presentation: Rajagopal, V.; Ferzoco, A. L. Gas Phase Fluorescence Spectroscopy for the Study of Catalytic Nitrogen-Containing Heterocycles, 64 th ASMS Conference on Mass Spectrometry and Allied Topics, June 2016. Poster presentation: Rajagopal, V.; Ferzoco, A. L. Gas Phase Fluorescence Spectroscopy for the Study of Catalytic Nitrogen-Containing Heterocycles, 64 th ASMS Conference on Mass Spectrometry and Allied Topics, June 2016. Poster presentation: Rajagopal, V.; Ferzoco, A. L. Characterization of Solvated and Unsolvated Fluorophores in Vacuum, American Chemical Society Fall 2015 National Meeting and Exposition, August 2015. Poster presentation: Rajagopal, V.; Ferzoco, A. L. Towards Probing Hydrogen Bonded Networks and Electron Transfer States Through the Characterization of Fluorophores, 63 rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2015. Poster presentation: Ko, Y. J.; Ferzoco, A. L. Development of a Liquid Phase Quadrupole Filter, 62 nd ASMS Conference on Mass Spectrometry and Allied Topics, June 2014. Poster presentation: Wooster, L.; Ko, Y. J.; Ferzoco, A. L. Development of an Inline Microfluidic Electrochemical Cell to Study Carbon Dioxide Reduction, 62 nd ASMS Conference on Mass Spectrometry and Allied Topics, June 2014. Poster presentation: Ko, Y. J.; Ferzoco, A. L. Modeling and Progress Towards a Segmented Linear Ion Trap for Spectroscopy of Gas Phase Ions, 61 st ASMS Conference on Mass Spectrometry and Allied Topics, June 2013. Poster presentation: Ko, Y. J.; Ferzoco, A. L. Modeling and Progress Towards a Segmented Linear Ion Trap for Spectroscopy of Gas Phase Ions, Gordon Research Conference on Gaseous Ions: Structures, Energetics & Reactions, February 2013. Poster presentation: Ferzoco, A. L.; Glish, G. L.; C-terminal Sequencing of Sodium Cationized FMOC Derivatized Peptides, 60 th ASMS Conference on Mass Spectrometry and Allied Topics, June 2011. Poster presentation: Ferzoco, A. L.; Spencer, S. E.; Arnold, M. L.; Steill, J.; Oomens, J.; Glish, G. L.; The Influence of an and an-17 ions on peptide dissociation pathways, 23 rd Sanibel Conference on Mass Spectrometry, January 2011. Oral presentation: Ferzoco, A. L.; Steill, J.; Oomens, J.; Bythell, B.; Paizs, B.; Glish, G. L. Sensitivity of b3 Ion Structure to Minor Changes in Amino Acid Sequence, 58 th ASMS Conference on Mass Spectrometry and Allied Topics, May 2010. Poster presentation: Ferzoco, A. L.; Ridgeway, M. E.; Kaplan, D. A.; Park, M. A.; Glish, G. L. "An In-Capillary FAIMS Device for High Transmission and Instrument Versatility," 57 th ASMS Conference on Mass Spectrometry and Allied Topics, May 2009. Poster presentation: Ferzoco, A. L.; Glish, G. L. "Measuring the Effective Temperature of Vibrationally Excited Ions," Triangle Area Mass Spectrometry Vendor Night, May 2008.
Poster presentation: Ferzoco, A. L.; Glish, G. L. "Measuring the Effective Temperature of Vibrationally Excited Ions," 56 th ASMS Conference on Mass Spectrometry and Allied Topics, May 2008. Oral presentation: Ferzoco, A. L.; Baker, E. S.; Gidden, J.; Bowers, M. T. "Formation of Helices in Oligonucleotides," The Lake Arrowhead Conference on Ion Chemistry and Mass Spectrometry, January 2004.