$ Optoelectronic Application of Transcutaneous Blood Oxygen Saturation and Heart Rate Monitoring

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1 !"#" $ Optoelectronic Application of Transcutaneous Blood Oxygen Saturation and Heart Rate Monitoring! "##$".!'(!.$ 911 -"." " tsawit@ratree.psu.ac.th $ " EFH ' J(K' 'H! ('H #" L-M" # KN 'OO! KH(H!''-'LE' '-'L' 'H #--'-' "E'H PNL O'H #--" PC16F876.K'O JH! ('OO! O "'KHE #( 'H! ( ± 5 F"E H# ± 1 J : -JJ, L-M", J, H! (, '"O! Abstract This paper presents the design and development of blood oxygen saturation and heart rate monitoring using dual-wavelength optoelectronic technique and transcutaneous method. The light sources were implemented by red and infrared LEDs whereas a single photodiode was employed for light detection. The captured signal was processed by PC16F876 microcontroller to display oxygen saturation and heart rate on LCD. The prototype achieved ± 5 beats/minute accuracy for heart rate and ± 1 SpO 2 accuracy for oxygen saturation. Keywords : Biosensor, Optoelctronic, Oxygen saturation, Heart rate, Transcutaneous method 1. '"."#PN.l.K H( m#"e#n ( #EF'H L#(HL""(!L J(K'-'# ne OO! JLo -'# "(!HONHPN NHM E L'# KH K' #O'H# 'M E 'H # ne Kp 'FL#(H'H ("' ONHLq #"E!' (!ONHLq #! (CU Lu$ E."# EFPO LL!H$! ('H E! m#'h#!!# K" ' L --" "(!HP#OLo "E" $H('H# LoH#."#!KONHE# ( LO PL#(H "#Ev 'N ONHN#'HE'H # 2. DEF 2.1 EH# [1] K'Lo$! (#"E#NJ E Lo KF#K'! E#NL 7-9 $F! L'H # 2! 'H FK'M'K' #EF(!H ( we-- (haemoglobin, Hb JE# $H!#( Lo L(M'K'' we--l'h #-LE"EE# we (heme - (globin JEL$n!M 'FK we-- J (O 2 'LoJwE-- (oxyhemoglobin, O 2 Hb JLo PE' KL#J (!HJH 'Lo'EJwE-- (deoxyhemoglobin, RHb JLo PE F!wE-- "E-J''Lo JwE-- (carboxyhemoglobin, COHb JLo PE '.N we--"e n!m"epnj'j '"we-- (methemoglobin, MetHb JLo PEF $H "FJwE-- "we--ne# P(J O' EF"(!H PE "EE # K '(NL"E 2.1

2 K# # (M OM M OM M!" OM M!"P!OM M NL"E 2.1 $we--( p 'H JwE--!KwE--"EEJ E'N'K # K (#E' EK ( #EFK"E '$E'.KEFL$J(K'! 'JOO!LoH '#O '"#.$ONHPN ' "EO F!$O! "E" ' 'FK (H '!' 2! "EE # K K!'"E#N(# 88 z 94 - (' 'H #! 3 L 'EF 1. "E ' JwE--E'N'K 'EJwE-- O # K#K 2. 'PN'N'K"EKF#K(HO! 'HH# EKp 3. PK(HL 'E# E''H KE # K(HE# 2.2 K#$ [2] K$"E$ #EFK"E ' OO! (transcutaneous method 'F!' PN'"K'"OO! F$KF#Kp LPwE-- O$'" KO!F KF#K'N'K"E # K ( A P { $ Beer-Lambert 'H C Lo!$O!!K $H$H KF#KK' l Lo#"L"nO!' PL K(HL"EH--'-' P $H"E'H (.n A = e (2.2 K $H$!' A 'N'K"E # K P ( R JLo.n d!.n'n'ke' ( A,RED.n dt d 'N'K' ( A,R LoL dt R = K R, max R,max R,max R,min R,min (2.3 Lo $HE'N' R, min ' ' Lo $HE'N' R,max R, min ' ' NL"E 2.2 'L $HN' ( max $H' ( "E'HL O min (2.3 PNL H# J(K' (SpO 2 -'##"E#O" blood-gas 'NL"E 2.3 J'H.nLo 1 55R SpO 2 = x1 ( R A = C l (2.1 i, j i, j i i K i, j Lo.$ # K '$we--' LoL"n'N'K$O! KF#KK' H ( max min (t NL"E 2.2 ' $HN'-'

3 SpO 2 ( Beer-Lambert $ Y " blood-gas R NL"E 3.1 L' NL"E 2.3.n! R SpO P MM #EF(H--" PC16F876.KL O '-'$'-'L P'O "JE'E 16 2 "' -'#.l-l Basic 'H # PCbasic PRO [3] 3. " 3.1 XX K"'L'~L-'-'L (LED --"J 'NL 3.1. O ' 'K L" {E K # K "E'HEP H # K 3 - P!'(!HLoL$H!'! $H (2.1 L K(HL TLS2[4] E"E # Kp" 'NL"E 3.2 "(!HH-L '#$'$E' "E'H TLS2 FLo.K HH-L##(- -".KL PELo'L E#.KF"E('M$HN HE# (L O"(!H ''HK #EF""'-'#(H! '!EL#F $ Dolphin -'#E"E'EF - 'E'"E # K 662 ± 'E'"E # K 95 ± ELo--'-'! '"EK(HEFPN '-'$'-'L --" $HO--'-' OLL O".LLo' $--" $ P! $ # (M NL"E 3.2 "E # Kp 3.2 "# MXX Differentiator /O port /V Amplifier LPF PC16F876 Microcontroller /O port RED, R LED Photodiode Summing Amp. A/D port LCD /O port NL"E 3.3 OO ( NL"E 3.3 'OO L'H # p 'EF - /V (!H#--'-' L 1 LÅ - Amplifier Lo $## 2 '' L$##'H 1-15 " - LPF Lo PEO' 2 "E PE' 3 wj E$##L 1 "

4 - Differentiator (H!N'$ (start.k(hh$! ( (!H-- -"M max LL OL - Summing Amp (H#'#N( z 5 - count =! EF(HLLÅ TL84 (uard OPAMP 2 K""' H 'H #K' PL$##'HL 9-1 " E F'P PE 3.8 wj J(H E#O " 'O'N'KE' ' ( '"H#$H--""E A/D port "E! $ start 'HO'NL"E pulse XX start 15 count P $$ count > 12 count = pulse XX start 3 count $ "" count "" count NL"E 3.6 ON# 'H$! ( l LED Y l LED NL"E 3.4 $H A/D start $E' XX start R,MAX P $ l LED l LED Y R,MAX min R,MN NL"E 3.5 $H A/D start $' 3.3 M (-L'H!'H!Lo K-#Lp H'H (HNLL Lo' 1 $HNJE'E 4 'LoON# $-L H'NL"E l LED Y l LED R,MN R,S Ox Sat NL"E 3.7 ON# ' SpO 2

5 3.3.1 H " 3.4 H ' $. H $! ( -L ( H H "E# Lo $ PCbasic Pro E PE ( pulsin P ( H 'H K E PE#N(.8 z 2 w J " F. ( 15 3 "E ( L count H!( $ min "' ' N "F Lo H #! O ' 2 ' ( LN "E 3.8 H " -L NL"E 3.8 ne! min K!! min. # $ -'#( H ne K 4 ( z ' NL"E 3.9 '! '"E( H NL"E 3.1 ' K H J E # - -".K ' ( L L.l ( ' L O K H L "' #-! #( -22 L " "' JF ( ( "E # 5 F E# P.! ( $ # (Exercise Stress Test, EST L E# "E# O K pulse oximetry.. $ Datex-Ohmeda TuffSat J ' H! ( 'H 4 z 225 F "E ' K H# 2 ' J 'H 7-1 SpO2 ' K H# 2 'E# 'HO ' L E# "E# ' ' ( NL"E 3.11 z3.14 NL"E 3.9! '"E( H( O ' 2 # EF $ n ( ' L $ PCbasic Pro E " # K"' ( NL $ M. "E 'H E O'. ' N ' F. ' ( " # -'#( H -L M (library FP32.NC Lo O (!H-L E$ ' (! $F Yo ( ' $ PCbasic Pro ( -L M "E. $H # E HL! -'# H (x K 1 < x 5 E E#'$ x Lo.5 J 'HO 8 P ( # E#' $ x Lo.25 -'#( H L ( " (!H P 'H # $F NL"E 3.1.P # 4 " K H H K (38 (34 (31 (28 3 (17 (19 (14 (12 1 "( p / NL"E 3.11 O ' M 'H H K " ( EF P( H (.KF "E $H N.E#. HL! -'# ( 65-1 SpO2 E#' 1 SpO2 ( $ SpO H $! ( (

6 "H K 15 1 (138!K--""E Ñ "E'E ' LP.l(!HE$'M.KP(H LoK..'H('L (22 (31 (1 (1 (3 (2 ( $# (p/ NL"E 3.12 'KH$! ( "H K (24 (24 (24 (24 (25 (18 (18 (8 (6 (4 (3 (3 (4 (3 (2 (2 (2 (2 (2 (2 ( SpO2 5. n $$ # E##- " ~ "E #'"'M$HN $$ LÜ 4 $ á LÜ 2545 "ELo "'OL # $$! "##$" "E(!H" #EF [1] E ", E "#!#(, E "# "#! "##$", [2] J.D. Bronzino (Editor-in-Chief, äthe Biomedical Engineering Handbookå, EEE press, [3] PCbasic Pro Compiler, MicroEngineering Labs nc., [4] TSL2 datasheet, Texas instruments, "H K (34 (11 (13 NL"E 3.13 O ' SpO 2 (18 (23 (19 (21(18 (15 (16 (3 (2 (2 (2 (2 ( ( $# ( SpO 2 NL"E 3.14 'KSpO 2 4. " O"'. KH 'H! ( -'#E'K ± 5 F/"E 'H# J'K H# ± 1 "FEFH LÑ# "EE O 'K$O ' " K!!"" ' K!M # LoH K!"E$ ' K. 'LÑ'L (truncation error $-L 'F(.l'LLo.l ne( L O JK(H-L.l, ONH ##,.. (.. (E."# Lo#- L -! "##$" E # ($L O.l K"."#,.. (.. ( Lo#L! "##$" E #($ L O

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