DNA Molecular Detection of Mycoplasmas: Introducing Real-Time PCR
|
|
|
- Andrew Holt
- 5 years ago
- Views:
Transcription
1 DNA Molecular Detection of Mycoplasmas: Introducing Real-Time PCR Pablo Lopez, DVM, MBA IDEXX Laboratories,Inc., Westbrook, Maine USA 2010 IDEXX Laboratories, Inc. All rights reserved.
2 Polymerase Chain Reaction (PCR) 1986 First description of the polymerase chain reaction - Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H. Cold Spring Harb Smp Quant Biol 1986; 51 Pt 1: First description of a thermostable DNA polymerase - Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Science 1988 Jan 29; 239(4839): First description of real-time polymerase chain reaction - Kinetic PCR analysis: real-time monitoring of DNA amplification reactions Higuchi R, Fockler C, Dollinger G, Watson R Biotechnology (NY) 1993 Sep; 11(9): Nobel Prize in Chemistry - Kary B. Mullis Polymerase Chain Reaction - Michael Smith Site-directed mutagenesis 1996 First commercial real-time PCR instruments available IDEXX Laboratories, Inc. All rights reserved.
3 Pathogenic Avian Mycoplasmas: Background Pathogenic avian mycoplasmas - Mycoplasma gallisepticum: chickens and turkeys - Mycoplasma synoviae: chickens and turkeys - Mycoplasma meleagridis: turkeys - Mycoplasma iowae: turkeys Disease symptoms - Airsacculitis, sinusitis - Synovitis, arthritis - Skeletal deformities - Embryo mortality - Poor performance Diagnosis - Culture: gold standard - Antibody detection: Serum Plate Agglutination (SPA), Hemagglutination Inhibition (HI), ELISA - Molecular detection: PCR IDEXX Laboratories, Inc. All rights reserved.
4 M. gallisepticum, M. synoviae, M. meleagridis Real Time PCR Assay Overview Hybridization probes detection chemistry - Crossing point and T m data values Surveillance (Screening) Testing - Crossing point value for yes/no determination Confirmatory Testing - Crossing point value - T m value for additional data confirmation of correct target detection Sequences for primers and probes based on amplicon sequences from S. Kleven - Avian Diseases 53: , Ziv Raviv and Stanley H. Kleven The Development of Diagnostic Real-Time TaqMan PCRs for the Four Pathogenic Avian Mycoplasmas IDEXX Laboratories, Inc. All rights reserved.
5 Hybridization Probes Detection Format Donor probe (D) provides energy to Acceptor probe (A) to emit signal when both probes are bound to target sequence (Fluorescence Resonance Energy Transfer) 3 primer probe 1 D A probe 2 D: Donor A: Acceptor 5 primer Probes are recycled throughout amplification program anneal Signals are collected by the instrument and used to calculate the crossing point values primer D 3 5 primer A probe 1 probe 2 Melting curves and T m values may be generated with hybridization probes IDEXX Laboratories, Inc. All rights reserved.
6 Cycling Program and Instrumentation M. gallisepticum, M. synoviae, M. meleagridis Real-Time PCR Instrumentation: LightCycler 480 with 96-well block Cycling Program: used for Mg, Ms and Mm real-time PCR assays - Activation: 1 cycle 95 C 10 minutes - Amplification: 45 cycles 95 C 20 seconds 60 C 20 seconds with a single fluorescence data acquisition 72 C 15 seconds - Melting Curve: 1 cycle 95 C 1 minute 45 C 1 minute 80 C continuous 0.14 C/second with collection of 4 fluorescence data acquisitions per second - Cooling: 1 cycle 40 C 30 seconds IDEXX Laboratories, Inc. All rights reserved.
7 Sample DNA Extraction Protocol used to generate data Tracheal Swabs and Mycoplasma Collection Broth High Pure PCR Template Preparation Kit (Roche Applied Science) -Spin column based format Trachael swabs: - Swabs were rinsed in 0.5 ml PBS ul PBS were used for the DNA extraction Mycoplasma Collection Broth: - 1 ml broth was centrifuged at 1000xg for 3 minutes - Pellet was resuspended in 200 ul PBS All DNA was stored at -80 C 5 µl Sample DNA was used per PCR assay reaction (total volume was 20 µl) IDEXX Laboratories, Inc. All rights reserved.
8 M. gallisepticum Amplicon Sequence Alignment with Wild Type mgc2 Sequences IDEXX Livestock and Poultry Diagnostics Consensus T T T T A T C C A G T A G T G G G T G C A G G T G C T G G G T T G A T T G T T G T T T C T T T A C T C T T G G G T T T A G G G A T T G G G A T T C C G A T C G C Mg amplicon AY AY AY AY AY AY Consensus Mg amplicon AY AY AY AY AY AY T T G G T T T A G G G A T T G G G A T T C C G A T C G C 29 T T T T A T C C A G T A G T G G G T G C A G G T G C T G G T T G A T T G T T G T T T C T T T A C T C T T G G T T T A G G G A T T G G G A T T C C G A T C G C 80 T T T T A T C C A G T A G T G G G T G C A G G T G C T G G T T G A T T G T T G T T T C T T T A C T C T T G G T T T A G G G A T T G G G A T T C C G A T C G C 80 T T T T A C C C A G T A G T G G G T G C A G G T G C T G G T T G A T C G T T G T T T C T T T A C T C T T G G T T T A G G G A T T G G G A T T C C G A T C G C 80 T T T T A C C C A G T A G T G G G C G C A G G T G C T G G T T G A T T G T T G T T T C T T T A C T C T T G G T T T A G G G A T T G G G A T T C C G A T C G C 80 T T T T A T C C A G T A G T G G G T G C A G G T G C T G G T T G A T T G T T G T T T C T T T A C T C T T G G T T T A G G G A T T G G G A T T C C G A T C G C 80 T T T T A T C C A G T A G T G G G T G C A G G T G C T G G T T G A T T G T T G T T T C T T T A C T C T T G G T T T A G G G A T T G G G A T T C C G A T C G C 80 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G A A T A A T C G A A G A A C T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G 95 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G A A T A A T C G A A G A A C 160 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G A A T A A T C G A A G A A C 160 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G A A T A A T C G A G C A A C 160 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G T A T A A T C G A A G A A C 160 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G A A T A A T T G A A G A A C 160 T A A G A A A A A A G A A A G A A T G A T G A T C C A A G A A C G T G A A G A A C A C C A A A A G A T G G T T G A A T C C C T T G G A A T A A T T G A A G A A C 160 AY and AY556290: Chicken isolates AY and Ay556276: Turkey isolates AY and AY : House Finch isolates All sequences from PDRC (N. Ferguson and S. Kleven) IDEXX Laboratories, Inc. All rights reserved.
9 M. synoviae Amplicon Sequence Alignment with Intergenic Spacer Region Sequences IDEXX Livestock and Poultry Diagnostics Consensus Ms amplicon AY FM FM FM AJ Consensus Ms amplicon AY FM FM FM AJ C T T A C G G A G T A C A T T A W T T T T A C A A A A G G C A T T T T T T A T C C T C C T T T C T T A C G G A G T A C A T T A T T T T T A C A A A A G G C A T T T T T T A T 47 A A G T C G T A A C A A G G T A T C C C T A C G A G A A C G T G G G G A T G G A T T A C C T C C T T T C T T A C G G A G T A C A T T A T T T T T A C A A A A G G C A T T T T T T A T C T T A C G G A G T A C A T T A W T T T T A C A A A A G G C A T T T T T T A T C T T A C G G A G T A C A T T A W T T T T A C A A A A G G C A T T T T T T A T C T T A C G G A G T A C A T T A W T T T T A C A A A A G G C A T T T T T T A T C T T A C G G A G T A C A T T A W T T T T A C A A A A G G C A T T T T T T A T 39 T A A C T G A A A G C T T T T A G A T T T T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T X G C C T T G G G C T A T T G T A T T T A G T T T T G A G A G A A C A A T A A C T G A A A G C T T T T A G A T T T T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T - G C C T T G G G C T A T T G T A T T T A G 119 T A A C T G A A A G C T T T T A G A T T T T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T - G C C T T G G G C T A T T G T A T T T A G T T T T G A G A G A A C A A 266 T A A C T G A A A G C T T T T A G A T T T T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T T G C C T T G G G C T A T T G T A T T T A G T T T T G A G A G A A C A A 126 T A A C T G A A A G C T T T T A G A T T T T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T T G C C T T G G G C T A T T G T A T T T A G T T T T G A G A G A A C A A 126 T A A C T G A A A G C T T T T A G A T T T T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T T G C C T T G G G C T A T T G T A T T T A G T T T T G A G A G A A C A A 126 T A A C T G A A A G C T T T T A G A K W A A A T T C T A A A A G C G G T T G T G T A T C G C T T T T T T T - G C C T T G G G C T A T T G T A T T T A G T T T T G A G A G A A C A A 128 AY768810: USDA FM213418: University of Liverpool (J. Bradbury) FM213412: University of Liverpool (J. Bradbury) FM213408: University of Liverpool (J. Bradbury) AJ781002: University of Liverpool (J. Bradbury) IDEXX Laboratories, Inc. All rights reserved.
10 M. meleagridis Amplicon Sequence Alignment with Intergenic Spacer Region Sequences IDEXX Livestock and Poultry Diagnostics Consensus A A C A A G G T A T C C C T A C G A G A A C G T G G G G A T G G A T T A C C T C C T T T C T A C G G A G T A C A T T A G T T A A T T T A T T T Mm amplicon AY AJ Consensus Mm amplicon AY AJ A A C A A G G T A T C C C T A C G A G A A C G T G G G G A T G G A T T A C C T C C T T T C T A C G G A G T A C A T T A G T T A A T T T A T T T 71 T T A A G T C G T A A C A A G G T A T C C C T A C G A G A A C G T G G G G A T G G A T T A C C T C C T T T C T A C G G A G T A C A T T A G T T A A T T T A T T T C T A C G G A G T A C A T T A G T T A A T T T A T T T 27 A A T C A T T G G G A C T A A A A T T A A C C T T T A A G C C T G T T A T T T T A A T A T T G T C A T G A C T T G G T T T A A G G C T C T G A G T X A T A T A T A A T C A T T G G G A C T A A A A T T A A C C T T T A A G C C T G T T A T T T T A A T A T T G T C A T G A C T T G G T T T A A G G C T C T G A G 143 A A T C A T T G G G A C T A A A A T T A A C C T T T A A G C C T G T T A T T T T A A T A T T G T C A T G A C T T G G T T T A A G G C T C T G A G T G A T A T A T 240 A A T C A T T G G G A C T A A A A T T A A C C T T T A A G C C T G T T A T T T T A A T A T T G T C A T G A C T T G G T T T A A G G C T C T G A G T T A T A T A T 107 AY762641: USDA AJ780999: University of Liverpool (J. Bradbury) IDEXX Laboratories, Inc. All rights reserved.
11 Analytical Sensitivity and Specificity IDEXX Laboratories, Inc. All rights reserved.
12 M. gallisepticum Real-Time PCR Analytical Sensitivity M. gallisepticum 100 pg 10 pg 1 pg 100 fg 10 fg 1 fg 0.1 fg NC Dilution CP Value T m value C 10 7 copies copies copies copies copies copies copies nc negative negative IDEXX Laboratories, Inc. All rights reserved.
13 M. synoviae Real-Time PCR Analytical Sensitivity Sample CP Value T m value 10 7 copies copies copies copies copies copies copies nc negative negative IDEXX Laboratories, Inc. All rights reserved.
14 M. meleagridis Real-Time PCR Analytical Sensitivity Sample CP Value T m value 10 6 copies copies copies copies copies copies nc negative negative IDEXX Laboratories, Inc. All rights reserved.
15 Analytical Specificity M. gallisepticum, M. synoviae, M. meleagridis M. gallisepticum M. synoviae M. meleagridis Sample DNA Crossing T m Crossing T m Crossing T m Point Point Point M. iowae negative negative negative negative negative negative M. lipofaciens negative negative negative negative negative negative M. cloacale negative negative negative negative negative negative M. imitans negative negative negative negative negative negative M. gallisnarum negative negative negative negative negative negative M. gallinaceum negative negative negative negative negative negative M. glycophilum negative negative negative negative negative negative M. pullorum negative negative negative negative negative negative M. gallopavonis negative negative negative negative negative negative 10 fg fg nc negative negative negative negative negative negative nc negative negative negative negative negative negative Non-pathogenic Mycoplasma DNA received from PDRC, University of Georgia All DNA tested negative with the three assays Assays did not recognize the non-pathogenic avian Mycoplasma DNA IDEXX Laboratories, Inc. All rights reserved.
16 Reproducibility IDEXX Laboratories, Inc. All rights reserved.
17 M. gallisepticum Real-Time PCR Inter-assay and Intra-assay Performance IDEXX Livestock and Poultry Diagnostics Assay 1 Assay 2 Assay 3 Dilution Crossing Crossing Crossing Crossing Crossing Crossing Crossing Crossing Crossing Standard Point Point Point Point Point Point Point Point Point Deviation Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 10 pg pg fg fg fg nc negative negative negative negative negative negative negative negative negative Assay 1 Assay 2 Assay 3 Dilution T m T m T m T m T m T m T m T m T m Standard Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Deviation 10 pg pg fg fg fg nc negative negative negative negative negative negative negative negative negative negative Standard Deviation is < 3% indicating acceptable inter-assay performance Target was M. gallisepticum plasmid ten-fold dilution series IDEXX Laboratories, Inc. All rights reserved.
18 M. synoviae Real-Time PCR Inter-assay and Intra-assay Performance Assay 1 Assay 2 Assay 3 Dilution Crossing Crossing Crossing Crossing Crossing Crossing Crossing Crossing Crossing Standard Point Point Point Point Point Point Point Point Point Deviation Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 10 pg pg fg fg fg nc negative negative negative negative negative negative negative negative negative Assay 1 Assay 2 Assay 3 Dilution T m T m T m T m T m T m T m T m T m Standard Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Deviation 10 pg pg fg fg fg nc negative negative negative negative negative negative negative negative negative Standard Deviation is < 3% indicating acceptable inter-assay performance Target was M. synoviae plasmid ten-fold dilution series IDEXX Laboratories, Inc. All rights reserved.
19 M. meleagridis Real-Time PCR Inter-assay and Intra-assay Performance Assay 1 Assay 2 Assay 3 Dilution Crossing Crossing Crossing Crossing Crossing Crossing Crossing Crossing Crossing Standard Point Point Point Point Point Point Point Point Point Deviation Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 10 pg pg fg fg fg nc negative negative negative negative negative negative negative negative negative Assay 1 Assay 2 Assay 3 Standard Dilution T m T m T m T m T m T m T m T m T m Deviation Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 Replicate 1 Replicate 2 Replicate 3 10 pg pg fg fg fg nc negative negative negative negative negative negative negative negative negative Standard Deviation is < 3% indicating acceptable inter-assay performance Target was M. meleagridis plasmid ten-fold dilution series IDEXX Laboratories, Inc. All rights reserved.
20 Performance IDEXX Laboratories, Inc. All rights reserved.
21 M. gallisepticum Real-Time PCR Sample Crossing T m Point Mg Mg Mg-3 negative negative Mg Mg-5 negative negative Mg DNA samples from chickens M. gallisepticum DNA positive 10 3 copies copies NC negative negative IDEXX Laboratories, Inc. All rights reserved.
22 M. gallisepticum Real-Time PCR Sample Crossing T m Sample Crossing T m Point Point Sample 1 negative negative Sample 17 negative negative Sample 2 negative negative Sample 18 negative negative Sample 3 negative negative Sample 19 negative negative Sample 4 negative negative Sample 20 negative negative Sample 5 negative negative Sample 21 negative negative Sample 6 negative negative Sample 22 negative negative Sample 7 negative negative Sample 23 negative negative Sample 8 negative negative Sample 24 negative negative Sample 9 negative negative Sample 25 negative negative Sample 10 negative negative Sample 26 negative negative Sample 11 negative negative Sample 27 negative negative Sample 12 negative negative Sample 28 negative negative Sample 13 negative negative Sample 29 negative negative Sample 14 negative negative Sample 30 negative negative Sample 15 negative negative Sample 31 negative negative Sample 16 negative negative Sample 32 negative negative DNA Samples from chickens M. gallisepticum negative 10 fg nc negative negative 1 fg nc negative negative IDEXX Laboratories, Inc. All rights reserved.
23 M. gallisepticum Real-Time PCR DNA samples from turkeys M. gallisepticum DNA positive DNA samples from turkeys M. gallisepticum DNA negative Sample Crossing T m Sample Crossing T m Point Point TK Tk-11 negative negative TK Tk-12 negative negative TK Tk-13 negative negative TK Tk-14 negative negative TK Tk-15 negative negative TK Tk-16 negative negative TK Tk-17 negative negative TK Tk-18 negative negative TK Tk-19 negative negative TK Tk-20 negative negative Tk-21 negative negative 10 fg Tk-22 negative negative 1 fg Tk-23 negative negative nc negative negative Tk-24 negative negative nc negative negative Tk-25 negative negative 10 fg fg nc negative negative nc negative negative IDEXX Laboratories, Inc. All rights reserved.
24 M. synoviae Real-Time PCR DNA samples from chickens M. synoviae DNA positive Sample Crossing T m Sample Crossing T m Sample Crossing T m Point Point Point MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH 7 negative negative MS CH MS CH MS CH 8 negative negative MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH MS CH fg MS CH MS CH fg MS CH MS CH nc negative negative MS CH MS CH nc negative negative IDEXX Laboratories, Inc. All rights reserved.
25 M. synoviae Real-Time PCR Sample Crossing T m Point MS 1 negative negative MS 2 negative negative MS 3 negative negative MS 4 negative negative MS 5 negative negative MS 6 negative negative MS 7 negative negative MS 8 negative negative MS 9 negative negative MS 10 negative negative MS 11 negative negative MS 12 negative negative MS 13 negative negative MS 14 negative negative MS 15 negative negative MS 16 negative negative DNA Samples from chickens M. synoviae negative nc negative negative 10 fg fg IDEXX Laboratories, Inc. All rights reserved.
26 M. synoviae Real-Time PCR Sample Crossing T m Sample Crossing T m Point Point MS TK 1 negative negative MS TK 32 negative negative MS TK 2 negative negative MS TK 33 negative negative MS TK 3 negative negative MS TK 34 negative negative MS TK 4 negative negative MS TK 35 negative negative MS TK 5 negative negative MS TK 36 negative negative MS TK 6 negative negative MS TK 37 negative negative MS TK 7 negative negative MS TK 38 negative negative MS TK 8 negative negative MS TK 39 negative negative MS TK 9 negative negative MS TK 40 negative negative MS TK 10 negative negative MS TK 41 negative negative MS TK 11 negative negative MS TK 42 negative negative MS TK 12 negative negative MS TK 43 negative negative MS TK 13 negative negative MS TK 44 negative negative MS TK 14 negative negative MS TK 45 negative negative MS TK 15 negative negative MS TK 46 negative negative MS TK 16 negative negative MS TK 47 negative negative MS TK 17 negative negative MS TK 48 negative negative MS TK 18 negative negative MS TK 49 negative negative MS TK 19 negative negative MS TK 50 negative negative MS TK 20 negative negative MS TK 51 negative negative MS TK 21 negative negative MS TK 52 negative negative MS TK 22 negative negative MS TK 53 negative negative MS TK 23 negative negative MS TK 54 negative negative MS TK 24 negative negative MS TK 55 negative negative MS TK 25 negative negative MS TK 56 negative negative MS TK 26 negative negative MS TK 57 negative negative MS TK 27 negative negative MS TK 28 negative negative 10 fg MS TK 29 negative negative 1 fg negative MS TK 30 negative negative nc negative negative MS TK 31 negative negative nc negative negative DNA Samples from turkeys M. synoviae negative IDEXX Laboratories, Inc. All rights reserved.
27 M. meleagridis Real-Time PCR Sample Crossing T m Point Sample Sample Sample Sample Sample Sample Sample Sample DNA Samples from turkeys M. Meleagridis positive 1 pg fg nc negative negative IDEXX Laboratories, Inc. All rights reserved.
28 M. meleagridis Real-Time PCR Sample Crossing T m Sample Crossing T m Point Point MM TK 1 negative negative MM TK 32 negative negative MM TK 2 negative negative MM TK 33 negative negative MM TK 3 negative negative MM TK 34 negative negative MM TK 4 negative negative MM TK 35 negative negative MM TK 5 negative negative MM TK 36 negative negative MM TK 6 negative negative MM TK 37 negative negative MM TK 7 negative negative MM TK 38 negative negative MM TK 8 negative negative MM TK 39 negative negative MM TK 9 negative negative MM TK 40 negative negative MM TK 10 negative negative MM TK 41 negative negative MM TK 11 negative negative MM TK 42 negative negative MM TK 12 negative negative MM TK 43 negative negative MM TK 13 negative negative MM TK 44 negative negative MM TK 14 negative negative MM TK 45 negative negative MM TK 15 negative negative MM TK 46 negative negative MM TK 16 negative negative MM TK 47 negative negative MM TK 17 negative negative MM TK 48 negative negative MM TK 18 negative negative MM TK 49 negative negative MM TK 19 negative negative MM TK 50 negative negative MM TK 20 negative negative MM TK 51 negative negative MM TK 21 negative negative MM TK 52 negative negative MM TK 22 negative negative MM TK 53 negative negative MM TK 23 negative negative MM TK 54 negative negative MM TK 24 negative negative MM TK 55 negative negative MM TK 25 negative negative MM TK 56 negative negative MM TK 26 negative negative MM TK 57 negative negative MM TK 27 negative negative MM TK 28 negative negative 10 fg MM TK 29 negative negative 1 fg MM TK 30 negative negative nc negative negative MM TK 31 negative negative nc negative negative DNA Samples from turkeys M. meleagridis negative IDEXX Laboratories, Inc. All rights reserved.
29 M. gallisepticum, M. synoviae, M. meleagridis Real-Time PCR Summary Sensitivity: Analytical sensitivity limit of detection is 10 copies of target sequence Specificity: Non-pathogenic Mycoplasma are not detected Data: - Crossing Point (CP): value indicates presence of target DNA - Tm: value confirms correct target sequence detected Clinical performance: Assays performed as expected - Mycoplasma negative chicken and turkey flocks were correctly identified - Mycoplasma positive chicken and turkey flock were correctly identified IDEXX Laboratories, Inc. All rights reserved.
LightCycler 480 Instrument Quick Guide Relative Quantification
LightCycler 480 Instrument Quick Guide Relative Quantification Performing a Relative Quantification Analysis: 1. Open the experiment that you want to analyze in main window 1. 2. If sample information
PRD (Poultry Respiratory Disease)-CAP
PRD (Poultry Respiratory Disease)-CAP Oct 24 th, 2018 Naola Ferguson-Noel, DVM, MAM, PhD Objective 2.1.3: Co-infection of broiler chickens with Mycoplasma synoviae, NDV, IBV and ILTV to reproduce airsacculitis
DEVELOP YOUR LAB, YOUR WAY
Technical brochure DEVELOP YOUR LAB, YOUR WAY The FLOW Solution: Expand your potential today Redesign your lab with the FLOW Solution The FLOW Solution is a highly flexible modular, semi-automated data
Color Compensation Set 1
Color Compensation Set 1 Version 2, March 2012 Solutions for the generation of color compensation objects for the LightCycler System. Order No. A 500 08 For 5 calibration runs. Store in dark at -15 to
RayBio Glucose Dehydrogenase Activity Assay Kit
RayBio Glucose Dehydrogenase Activity Assay Kit User Manual Version 1.0 January 23, 2015 RayBio Glucose Dehydrogenase Activity Assay (Cat#: 68AT-GluD-S100) RayBiotech, Inc. We Provide You With Excellent
Monensin ELISA Kit. Catalog Number KA assays Version: 11. Intended for research use only.
Monensin ELISA Kit Catalog Number KA1422 96 assays Version: 11 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General Information...
Unleash the Power of Real-Time PCR
The LightCycler 480 Real-Time PCR System Unleash the Power of Real-Time PCR Steven van Konynenburg Field Applications Specialist LightCycler 480 System Overview 1. General Description 2. LightCycler 480
ab PicoProbe ALDH Activity Assay Kit Instructions for Use
ab155894 PicoProbe ALDH Activity Assay Kit Instructions for Use For the sensitive and accurate measurement of Aldehyde Dehydrogenase activity in cell and tissue culture supernatants, cell lysates, plasma,
Microbial DNA qpcr Multi-Assay Kit Clostridium perfringens Pathogenicity
Microbial DNA qpcr Multi-Assay Kit Clostridium perfringens Pathogenicity Cat. no. 330043 BBID-1507ZR-3 For real-time PCR-based, application-specific microbial identification or profiling The Clostridium
Colorimetric GAPDH Assay Cat. No. 8148, 100 tests
Colorimetric GAPDH Assay Cat. No. 8148, 1 tests Introduction Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for
Pyruvate Kinase Assay Kit
Pyruvate Kinase Assay Kit Catalog Number KA0873 100 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
ALDH Activity Assay Kit (Colorimetric)
ab155893 ALDH Activity Assay Kit (Colorimetric) Instructions for Use For the sensitive and accurate measurement of ALDH activity in cell and tissue culture supernatants, cell lysates, plasma and serum.
Data Sheet. Azide Cy5 RNA T7 Transcription Kit
Cat. No. Size 1. Description PP-501-Cy5 10 reactions à 40 µl For in vitro use only Quality guaranteed for 12 months Store all components at -20 C. Avoid freeze and thaw cycles. DBCO-Sulfo-Cy5 must be stored
GGT Activity Assay Kit (Colorimetric)
GGT Activity Assay Kit (Colorimetric) Catalog Number KA3746 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
mrna Isolation Kit for Blood/Bone Marrow For isolation mrna from blood or bone marrow lysates Cat. No
For isolation mrna from blood or bone marrow lysates Cat. No. 1 934 333 Principle Starting material Application Time required Results Key advantages The purification of mrna requires two steps: 1. Cells
Alcohol Dehydrogenase Detection Kit
ab102533 Alcohol Dehydrogenase Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Alcohol Dehydrogenase activity in various samples This product is for research use
Lactate Dehydrogenase Assay Kit
Lactate Dehydrogenase Assay Kit Catalog Number KA0878 500 assays Version: 10 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
Arginase Assay Kit. Catalog Number KA assays Version: 05. Intended for research use only.
Arginase Assay Kit Catalog Number KA1609 200 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
RayBio Human IDUA ELISA Kit
RayBio Human IDUA ELISA Kit Catalog #: ELH-IDUA User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric)
ab102526 Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Lactate Dehydrogenase in various samples. This product is for research
U.S. Patent No. 9,051,563 and other pending patents. Ver
INSTRUCTION MANUAL Direct-zol 96 RNA Catalog Nos. R2054, R2055, R2056 & R2057 Highlights Quick, 96-well purification of high-quality (DNA-free) total RNA directly from TRIzol, TRI Reagent and all other
For the sensitive and accurate measurement of α-glucosidase activity in a variety of samples
ab174093 Alpha-Glucosidase Activity Assay Kit (Colorimetric) Instructions for Use For the sensitive and accurate measurement of α-glucosidase activity in a variety of samples This product is for research
09/07/16 12/07/16: 14/07/16:
09/07/16 Transformation of DH5 alpha with the InterLab transformation protocol : DNA constructions were dried, so we resuspended them into 100µL nuclease-free water. We took 5µL of the product for 25µL
Automated purification of high quality genomic DNA
APPLICATION NOTE No. AA267 I October 2012 Automated purification of high quality genomic DNA from various tissues using the Eppendorf MagSep Tissue gdna Kit on the Eppendorf epmotion M5073 Ulrich Wilkening,
Alpha Ketoglutarate (alpha KG) Assay Kit
ab83431 Alpha Ketoglutarate (alpha KG) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of alpha Ketoglutarate in various samples This product is for research use only and
RayBio Human Thyroid Peroxidase ELISA Kit
RayBio Human Thyroid Peroxidase ELISA Kit Catalog #: ELH-TPX User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100
For the sensitive and accurate measurement of peroxide activity in biological samples.
ab155895 Peroxidase Activity Assay Kit Instructions for Use For the sensitive and accurate measurement of peroxide activity in biological samples. This product is for research use only and is not intended
ALDH Activity Assay Kit (Colorimetric)
ALDH Activity Assay Kit (Colorimetric) Catalog Number KA3742 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
For the quantitative measurement of PEGylated molecules in plasma, serum and cell culture media
ab138914 Polyethylene Glycol (PEG) ELISA Kit Instructions for Use For the quantitative measurement of PEGylated molecules in plasma, serum and cell culture media This product is for research use only and
MLVA Update. Eija Trees, Ph.D., D.V.M. PulseNet Methods Development and Reference Unit Enteric Diseases Laboratory Branch CDC, Atlanta, GA
MLVA Update Eija Trees, Ph.D., D.V.M. PulseNet Methods Development and Reference Unit Enteric Diseases Laboratory Branch CDC, Atlanta, GA Presentation outline Status of the S. Enteritidis MLVA protocol
Mag-Bind Soil DNA Kit. M preps M preps M preps
Mag-Bind Soil DNA Kit M5635-00 5 preps M5635-01 50 preps M5635-02 200 preps January 2013 Mag-Bind Soil DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
mag nanogram kit Description Kit uses
mag nanogram kit Catalogue number 40701 and 40710 (For research use only. Not for use in diagnostic procedures.) Description mag kits use magnetic separation for the preparation of nucleic acids. Superparamagnetic
Phosphoenolpyruvate Assay Kit
Phosphoenolpyruvate Assay Kit Catalog Number KA3745 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
This manual must be read attentively and completely before using this product.
(FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSTICS!) Elabscience OT(Oxytocin) ELISA Kit Synonyms: OT Catalog No : E-EL-0029 96T This manual must be read attentively and completely before using
RayBio Rat TNF-alpha ELISA Kit (For Lysates)
RayBio Rat TNF-alpha ELISA Kit (For Lysates) Catalog #: ELR-TNFa-CL User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
For the rapid, sensitive and accurate measurement of Pyruvate Kinase (PK) activity in various samples.
ab83432 Pyruvate Kinase (PK) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Pyruvate Kinase (PK) activity in various samples. This product is for research use only
ADH Activity Assay Kit
ADH Activity Assay Kit Catalog Number KA3713 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
RayBio CaspGLOW TM Fluorescein Active Caspase-3 Staining Kit
RayBio CaspGLOW TM Fluorescein Active Caspase-3 Staining Kit User Manual Version 1.0 May 10 th, 2015 RayBio Caspase-3 Fluorometric Assay Kit Protocol (Cat#: 68FLS-Casp3-S) RayBiotech, Inc. We Provide You
Hydrogen Peroxide Colorimetric Detection Kit
Hydrogen Peroxide Colorimetric Detection Kit Catalog Number KA1017 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the
genesig Easy q16 Validation kit
Primerdesign TM Ltd genesig Easy q16 Validation kit For use on the genesig q16 50 reactions For general laboratory and research use only. 1 genesig Easy: at a glance guide For each validation test Component
Xanthine Oxidase Assay Kit
Xanthine Oxidase Assay Kit Catalog Number KA0874 100 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
IgG (Rabbit) ELISA Kit
IgG (Rabbit) ELISA Kit Catalog Number KA2017 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
U.S. Patent No. 9,051,563 and other pending patents. Ver
INSTRUCTION MANUAL Direct-zol RNA MiniPrep Catalog Nos. R050, R05, R05, & R053 Highlights Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol, TRI Reagent and other
FOR RESEARCH USE ONLY.
MAN-10039-04 Vantage 3D DNA SNV Qualification Kit Vantage 3D DNA SNV Qualification Kit The ncounter Vantage 3D DNA SNV Qualification Kit is designed to assess whether the ncounter MAX, FLEX, or SPRINT
Xanthine Oxidase Assay Kit
ab102522 Xanthine Oxidase Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Xanthine Oxidase activity in various samples. This product is for research use only and is
Catalase Assay Kit. Catalog Number KA assays Version: 04. Intended for research use only.
Catalase Assay Kit Catalog Number KA0884 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials Supplied...
Procine GHb ELISA Kit
Procine GHb ELISA Kit For the quantitative in vitro determination of Procine Glycated hemoglobin concentrations in serum - plasma - celiac fluid - tissue homogenate - body fluid FOR LABORATORY RESEARCH
Lactate Dehydrogenase Assay Kit
Lactate Dehydrogenase Assay Kit Catalog Number KA0878 500 assays Version: 08 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
Oxaloacetate Assay Kit
ab83428 Oxaloacetate Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Oxaloacetate levels in various samples This product is for research use only and is not intended
Carcino embryonic Antigen Human ELISA Kit
ab108635 Carcino embryonic Antigen Human ELISA Kit Instructions for Use For the quantitative measurement of Human Carcino embryonic Antigen (CEA) concentrations in serum. This product is for research use
Catalase Fluorometric/Colorimetric Assay Kit
Catalase Fluorometric/Colorimetric Assay Kit Catalog No. KM0044 Detection and Quantification of Catalase Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic or Clinical
C3 (Mouse) ELISA Kit. Catalog Number KA assays Version: 15. Intended for research use only.
C3 (Mouse) ELISA Kit Catalog Number KA1926 96 assays Version: 15 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
Prealbumin (Mouse) ELISA KitI
Prealbumin (Mouse) ELISA KitI Catalog Number KA2070 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of
Gyrolab Protein A Kit Quantification of residual Protein A ligands in the presence of excess amounts of IgG
Quantification of residual Protein A ligands in the presence of excess amounts of IgG Product number P0020456 P0020457 Product Name Gyrolab Protein A Kit for MabSelect SuRe Ligand Gyrolab Protein A Kit
Application Note: A TD-700 Laboratory Fluorometer Method for Alkaline Phosphatase Fluorescence
1. INTRODUCTION Because of their critical functions in eukaryotic cells, methods for measuring protein phosphatases were established at least as early as 1953 1. In 1965 Fernley and Walker 2 decribed the
For the rapid, sensitive and accurate measurement of Catalase activity in various samples.
ab83464 Catalase Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Catalase activity in various samples. This product is for research use only and is not intended for
ab IgA Cat ELISA Kit
ab190547 IgA Cat ELISA Kit Instructions for Use For the quantitative measurement of IgA in Cat serum and plasma. This product is for research use only and is not intended for diagnostic use. Version 3
ab C Reactive Protein (PTX1) Dog ELISA Kit
ab157698 C Reactive Protein (PTX1) Dog ELISA Kit Instructions for Use For the quantitative measurement of C Reactive Protein (PTX1) in Dog serum and plasma samples. This product is for research use only
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Complement CH50 Assay. Kit Insert
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Complement CH0 Assay Kit Insert Version: November 01 Summary Complement hemolytic activity is a functional test of the classical
HUMAN MYELOPEROXIDASE
HUMAN MYELOPEROXIDASE Immunoperoxidase Assay for Determination of Myeloperoxidase in Human Samples DIRECTIONS FOR USE Version3 GB0073 For Research Use Only, NOT for Diagnostic Purposes Please Read this
Antioxidant Assay Kit
Antioxidant Assay Kit Catalog Number KA1622 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
Caspase Substrate Assay Kit (Colorimetric)
Caspase Substrate Assay Kit (Colorimetric) Catalog Number KA3694 7 x 25 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
ab Ferritin (FTL) Rat ELISA Kit
ab157732 Ferritin (FTL) Rat ELISA Kit Instructions for Use For the quantitative measurement of Ferritin (FTL) in rat biological samples. This product is for research use only and is not intended for diagnostic
CATALASE ACTIVITY ASSAY KIT KB test (96 well plate)
CATALASE ACTIVITY ASSAY KIT KB-03-012 100 test (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Sample preparation Pag. 4 Reagents preparation Pag. 6 Assay protocol Pag.
ab IgA Pig ELISA Kit
ab190536 IgA Pig ELISA Kit Instructions for Use For the quantitative measurement of IgA in pig serum and plasma. This product is for research use only and is not intended for diagnostic use. Version 4
Alcohol dehydrogenase Assay Kit
Alcohol dehydrogenase Assay Kit Catalog Number KA3785 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General
Applications Note 215 September 2009
Applications Note 215 September 2009 Technical Report Performance evaluation of white Eppendorf Polypropylene Microplates using the Promega CellTiter-Glo Luminescent Assay Natascha Weiß 1, Daniel Wehrhahn
CA125 (Human) ELISA Kit
CA125 (Human) ELISA Kit Catalog Number KA0205 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
Phospholipase C Fluorometric/Colorimetric Assay Kit
Phospholipase C Fluorometric/Colorimetric Assay Kit Catalog No. KM0129 Detection and Quantification of Phospholipase C Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic
NAD + /NADH Assay [Colorimetric]
![NAD + /NADH Assay [Colorimetric]](/thumbs/91/105496846.jpg "NAD + /NADH Assay [Colorimetric]")
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name NAD + /NADH Assay [Colorimetric] (Cat. #786 1539, 786 1540) think proteins! think G-Biosciences
CSA: Simian Panel E. (Product Number ) User Protocol. 1.0 Introduction Kit Contents Required Materials...
L757 28Jun2018 1 of 9 Table of Contents 1.0 Introduction... 2 2.0 Kit Contents... 3 3.0 Required Materials... 3 4.0 Storage... 3 5.0 Safety and Handling... 4 6.0 Protocol Overview... 4 7.0 Procedure...
RayBio Human VEGFR1 ELISA Kit
RayBio Human VEGFR1 ELISA Kit Catalog #: ELH-VEGFR1 User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
RayBio Human LBP ELISA Kit
RayBio Human LBP ELISA Kit Catalog #: ELH-LBP User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA
Sulfonamide Residues (Three) ELISA Quantitation Kit. Manual
Sulfonamide Residues (Three) ELISA Quantitation Kit Manual Catalog number: 40-784-140023 Immunoperoxidase Assay for Determination of Sulfamerzine (SM1), Sulfanethazine (SM2), Sulfadimethoxine (SDM) in
RayBio Human CA-125 ELISA Kit
RayBio Human CA-125 ELISA Kit Catalog #: ELH-CA125 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
Michigan State University Diagnostic Center for Population and Animal Health, Lansing MI USA. QIAGEN Leipzig GmbH, Leipzig, Germany
Detection of Bovine Viral Diarrhea (BVD) Virus in Ear Skin Tissue Samples: Evaluation of a Combination of QIAGEN MagAttract 96 cador Pathogen Extraction and virotype BVDV RT-PCR-based Amplification. Roger
Instructions for use. Chromogranin A ELISA TM E-9000
Instructions for use Chromogranin A ELISA TM E-9000 Chromogranin A ELISA 1. Intended use and principle of the test Enzyme Immunoassay for the quantitative determination of Chromogranin A in serum and plasma.
Mouse Ferritin ELISA
K-ASSAY PRODUCT DATA SHEET Mouse Ferritin ELISA For the quantitative determination of ferritin in mouse biological samples Cat. No. KT-396 For research use only. 1 Rev. 021808 K-ASSAY PRODUCT INFORMATION
Superoxide Dismutase Activity Assay Kit
Superoxide Dismutase Activity Assay Kit Catalog Number KA0783 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
Acid Phosphatase Assay Kit (Colorimetric)
Acid Phosphatase Assay Kit (Colorimetric) Catalog Number KA0816 500 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
Paraquat ELISA Kit. Catalog Number KA assays Version: 17. Intended for research use only.
Paraquat ELISA Kit Catalog Number KA1424 96 assays Version: 17 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General Information...
Glutamate Dehydrogenase Assay Kit
Glutamate Dehydrogenase Assay Kit Catalog Number KA0879 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4
Human Creatinine Serum Detection Kit
Human Creatinine Serum Detection Kit Catalog No: IRAAKT2508 Lot No: SAMPLE INTENDED USE The Serum Creatinine kit is designed to quantitatively measure creatinine present in serum samples. Please read the
IgG (Cat) ELISA Kit. Catalog Number KA assay Version: 01. Intend for research use only.
- IgG (Cat) ELISA Kit Catalog Number KA2048 96 assay Version: 01 Intend for research use only www.abnova.com Introduction and Background A. Test principle The principle of the double antibody sandwich
Reagents Quantity Reagents Quantity. Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4. Standard 2 Standard Diluent 1 20mL
SEB932Hu 96 Tests Enzyme-linked Immunosorbent Assay Kit For Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Organism Species: Homo sapiens (Human) Instruction manual FOR RESEARCH USE ONLY NOT FOR USE
RayBio Human Semaphorin 6D ELISA Kit
RayBio Human Semaphorin 6D ELISA Kit Catalog #: ELH-SEMA6D User Manual Last revised February 9, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100
Human Lipocalin-2/NGAL ELISA
K-ASSAY Human Lipocalin-2/NGAL ELISA For the quantitative determination of Lipocalin-2/NGAL in human biological samples Cat. No. KT-542 For Research Use Only. 1 Rev. 13453542 K-ASSAY PRODUCT INFORMATION
Human Myeloperoxidase ELISA
K-ASSAY Human Myeloperoxidase ELISA For the quantitative determination of myeloperoxidase in human serum or plasma Cat. No. KT-342 For research use only. Not for use in diagnostic procedures. 1 Rev. 051208
HP (Rabbit) ELISA Kit
HP (Rabbit) ELISA Kit Catalog Number KA1930 96 assays Version: 01 Intended for research use only www.abnova.com Introduction and Background A. Introduction Acute phase proteins are plasma proteins, which
Endotoxin ELISA Kit (Competitive EIA)
Endotoxin ELISA Kit (Competitive EIA) Catalog No. DEIAPY07261 It is important that you read this entire manual carefully before starting your experiment. This kit is for Research Use Only. Not for Diagnostic
Acetylcholinesterase Assay Kit
Acetylcholinesterase Assay Kit Catalog Number KA1607 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay...
Xingxing Ren, Ying Fu, Chenggang Xu, Zhou Feng, Miao Li, Lina Zhang, Jianmin Zhang, 1 and Ming Liao 1
High resolution melting (HRM) analysis as a new tool for rapid identification of Salmonella enterica serovar Gallinarum biovars Pullorum and Gallinarum Xingxing Ren, Ying Fu, Chenggang Xu, Zhou Feng, Miao
NukEx Nucleic Acid Release Reagent
Instruction for Use NukEx Nucleic Acid Release Reagent For general laboratory use. For in vitro use only. Reagent for the enzymatic release of nucleic acid from tissue samples, ticks, insects and swabs.
Glutathione Peroxidase Assay Kit
Glutathione Peroxidase Assay Kit Catalog Number KA1628 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
NAD/NADH Assay Kit. Catalog Number KA assays Version: 03. Intended for research use only.
NAD/NADH Assay Kit Catalog Number KA3777 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...
Ion Sphere Assay on the Qubit 3.0 Fluorometer
USER GUIDE Ion Sphere Assay on the Qubit 3.0 Fluorometer for use with: Ion Sphere Quality Control Kit (Cat. No. 4468656) Publication Number MAN0016388 Revision A.0 Ion Sphere Assay overview... 2 Materials
IgG (Chicken) ELISA Kit
IgG (Chicken) ELISA Kit Catalog Number KA2426 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the
KAMIYA BIOMEDICAL COMPANY. Cat IgE ELISA. For the quantitative determination of IgE in cat biological samples. Cat. No. KT-394. For Research Use Only.
K-ASSAY KAMIYA A BIOMEDICAL COMPANY KAMIYA BIOMEDICAL COMPANY Cat IgE ELISA For the quantitative determination of IgE in cat biological samples Cat. No. KT-394 For Research Use Only. 1 Rev. 020708 K-ASSAY
IgE (Dog) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only.
IgE (Dog) ELISA Kit Catalog Number KA1937 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General Information...
RayBio MMP-3 Activity Assay Kit
RayBio MMP-3 Activity Assay Kit User Manual Version 1.0 Mar 26, 2014 RayBio MMP-3 Activity Assay (Cat#: 68AT-MMP3-S100) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll Free)1-888-494-8555