Effect of Methods of Platelet Resuspension on Stored Platelets
|
|
- Clifford Jacobs
- 6 years ago
- Views:
Transcription
1 ANNALS O F CLINICAL AND LABORATORY S C IEN C E, Vol. 14, No. 5 Copyright 1984, Institute for Clinical Science, Inc. Effect of Methods of Platelet Resuspension on Stored Platelets THOMAS KIRALY, M.A., S.B.B. (ASCP), SUSAN BERNIER, M.S., M.T. (ASCP) S.B.B., R. M. KAKAIYA, M.D., and RITCHARD G. CABLE, M.D. American Red Cross Blood Services, Farmington, CT ABSTRACT Platelets are prepared from whole blood by differential centrifugation. Following their isolation as a platelet button, platelets are allowed to rest for a short period in the residual plasma before resuspension. In this study, the feasibility of resuspending platelets without this rest period is studied. A total of 35 platelet concentrates (PC) were prepared from blood collected in CPDA-1 and resuspended by one of the following four methods: (1) no resting period, PC placed on a rotator immediately after preparation, (2) a 1.5 hour rest period and gentle shaking prior to rotation, (3) no rest period and immediate gentle shaking prior to rotation, and (4) a 1.5 hour resting phase and no shaking prior to rotation. Following the previous processing, all platelet concentrates were stored for 72 hours on an elliptical platelet rotator at 20 to 24 C to provide continuous agitation. A number of in vitro tests were used as indicators of platelet viability during storage. These included platelet morphology, p 0 2, p C 0 2, ph, osmotic recovery, number of platelets in the concentrate, and platelet volume distribution. O ur findings dem onstrate that platelets are of similar quality after storage in all of the four groups described. Our studies suggest that the resting phase is unnecessary for platelet preparation. The elimination of the resting phase would allow platelet concentrates to be available sooner and improve cost-effectiveness of platelet preparation. Introduction Platelets are generally prepared from anticoagulated whole blood by differential centrifugation.811 The first spin produces platelet rich plasma (PRP) which is transferred to an integrally connected satellite bag. A second spin produces a unit of platelet poor plasma (PPP) and a platelet button. After the removal of all but 50 to 60 ml of PPP, the current general practice is to allow the platelet button to rest undisturbed for 60 to 90 m inutes before resuspension.8 At this point in preparation, the platelet concentrates are then placed in a rotator to allow continuous agitation during storage at room temperature. The rationale for the resting phase in platelet preparation comes from the /84/ $00.90 Institute for Clinical Science, Inc.
2 studies performed by Mourad almost 15 years ago.6,7 He reported that the release of platelet enzymes was proportional to the degree of aggregate formation after the centrifugation of platelets. Platelets allowed to rest for 20 to 60 minutes before being rotated were free of aggregates and had decreased enzyme levels when compared to non-rested platelets. More recently, Snyder et al.14 in collaboration with our center, have found that platelets allowed to rest for 90 minutes had a lower B-TG and LDH release at 24 hour storage than platelets not allowed to rest. However, at 72 hour storage, the B-TG and LDH discharge was independent of the rest period. Katz et al.4 have examined immediate resuspension of pheresis platelets by manual agitation and found no detrim ental effect. In previous studies, the effect of a rest period on other parameters, such as ph, morphology, blood gases, and recovery to osmotic stress during storage have not been examined ,12 Since many of these parameters have been useful in assessing the quality of stored platelets, these studies are perform ed to determine the effect of eliminating the rest period. Materials and Methods P l a t e l e t P r e p a r a t io n Blood from 35 normal blood donors was collected into a closed triple bag system* containing CPDA-1 as anticoagulant. P latelet concentrates were prepared within four hours of blood collection using a centrifuge, f To prepare platelet rich plasma (PRP), the blood was centrifuged at 2800 rpm (2000 X g) for * PL-146, Fenwal, Deerfield, IL. t Sorval RC-3, Sorval, Newtown, CT. $ Fenwal, Deerfield, IL. METHODS OF PLATELET RESUSPENSION TABLE-I Methods of Platelet Resuspension G roup A * Group B n= 9 Group C n=8 G roup D 1.5 H our r e s t Manual A g ita tio n Hour r o t a t i o n *n = Number of samples. 2.7 minutes. To harvest a platelet button from PRP, the PRP was centrifuged at 4200 rpm (4500 x g) for five minutes. All but approximately 60 ml of platelet poor plasma (PPP) was transferred out to another satellite bag, leaving sufficient PPP to resuspend the platelet button. R e s u s p e n s i o n P r o t o c o l s These protocols are presented in table I. As seen in table I, platelet concentrates in the control group (group D) and in Group B were allowed to rest on a flat surface at am bient tem perature for 90 minutes, while platelet concentrates in Group A and C were placed on the rotator im m ediately after preparation. Platelet concentrates in groups B and C were resuspended by gentle manual agitation prior to rotation. All PC were rotated at six rpm on an elliptical rotator^ in an environm entally controlled chamber at 22 to 24 C. S t o r a g e S t u d i e s Following resuspension, platelets were stored as described for 72 hours. At various storage intervals, ph, po, pc 02, platelet morphology score, platelet recovery to osmotic stress, and cell counts were measured as previously described.3 Platelet size distribution was m easured according to the m ethod of Holme et al.2 Significant differences between groups were performed by one way analysis of
3 3 6 8 KIRALY, BERNIER, KAKAIYA, AND CABLE TABLE I I ph, PO2, and pc02 Measurements on Platelets Stored for 72 Hours Group A G roup B Group C G roup D PH 2 Hour 7. 09±0.01* 7. 06± ± Hour 6. 79± ± ± ± Hour ± ± ±0.39 po2 2 Hour 6 6.9± ± ± ± Hour ± Hour ± ± ±26.7 PC02 2 Hour 5 8.4± ± ± ± Hour 7 8.3± ± ± Hour 6 2.9± ± ± ± 18.2 * M ean ± 1 S. D. p > 0.05 in a l l com parisons betw een groups. variance and student-n ew m an-k euls test.10 Results During the storage period, there was no difference betw een the groups and the volume of the concentrate (data not shown). The volume of PRP in all concentrates decreased by about 20 percent after 72 hours of storage because of repeated sampling. There was also no statistically significant change in platelet count over the storage period and no statistically significant difference between the groups at any tim e; m ean platelet counts ranged from X 106 per nl ph D u r i n g S t o r a g e As shown in table II, the mean ph at T = 2 for the four groups ranged from 7.05 to A decrease in ph occurred during storage in all groups. However, there were no statistically significant differences in ph between the groups at any storage interval. B l o o d G a s e s p 0 2 and pc 02 values during storage are shown in table II. In all groups, there was an initial decline in p 0 2 at 24 hours followed by an increase in p 0 2 at 72 hours. Initially, p C 0 2 increased (at T = 24 hours) but subsequently declined at 72 hours storage in all the groups. There was no statistically significant difference between the groups for p 0 2 or p C 0 2 at any time. P l a t e l e t M o r p h o l o g y D u r in g S t o r a g e Morphology scores for each group at various storage intervals are shown in table III. While morphology declined in each group, there were no statistically significant differences in morphology scores between the groups at any storage interval. R e c o v e r y t o O s m o t ic S t r e s s D u r i n g S t o r a g e The mean ± SD recovery to osmotic stress after 72 hours of storage was as follows: group A = 53.9 ± 15.5 percent, group B = ±6.7 percent, group C = 59.0 ± 13.8 percent, and group D = 50.3 ± 11.2 percent. There were no statistically significant differences between the groups. M e d i a n P l a t e l e t V o l u m e D u r i n g S t o r a g e Median platelet volume was measured at T = 0 and T = 72 for each group. The TABLE III Effect of Different Resuspension Methods on Platelet Morphology During 72 Hour Storage S t o r a g e T im e (H o u r s ) G ro u p A * n - 9 G ro u p B G ro u p C n ~ 8 G ro u p D n= ±96t 572±54 532±85 577± ± ± ±57 521± ±45 *n = Number o f sa m p le s. tm ean ± 1 S.D. p > f o r a l l co m p a riso n s betw een g ro u p s.
4 TABLE IV V a r ia tio n i n th e M edian P l a t e l e t Volume Over 72 Hours of S to rage S t o r a g e Tim e (H o u rs) G roup A * G roup B n - 9 G roup C n=8 G roup D im m ediate 6. 4± 1. Ot ± *n = Number o f sa m p le s. tm ean p l a t e l e t volum e i n f l. ± 1 S.D. p > 0.05 fo r com parisons betw een grou ps. values are shown in table IV. Again, no statistically significant differences were observed. Discussion The purpose of this study was to determine what effect, if any, the elimination of the resting phase during platelet concentrate preparation and resuspension would have on subsequent storage of platelet concentrates. Previous studies have exam ined the release of platelet constituents during p latelet preparation.6,714 A num ber of other in vitro parameters have been studied to delineate further this aspect of platelet preparation. Four resuspension methods have been investigated, two of which utilized a rest period and two which did not. One group of platelet concentrates was resuspended after the rest period with gentle manual agitation while the other group of PC was simply placed on a mechanical rotator after the rest period. Similarly, in platelet concentrates in which no rest period was allowed, one group was resuspended manually prior to rotation and the other group simply placed on a rotator. Our studies show that the ph was well maintained during 72 hours of storage, regardless of how platelets were resuspended. P latelet m orphology and re covery to osmotic stress were also well m aintained over the storage interval. Changes in p 0 2 and p C 0 2 w ere in d e METHODS OF PLATELET RESUSPENSION pendent of resuspension method and are sim ilar to those reported previously.3 Volume distribution curves w ere also comparable in all four groups. These results show that there are no discernable ill effects of om itting the resting phase from platelet preparation and no appreciable benefit to manual agitation prior to rotation. The rationale for using a rest period after platelets are harvested has been to facilitate a smooth resuspension of platelets. Although aggregates were not quantitated in platelet concentrates, visual inspection of platelet concentrations after preparation and during storage showed no differences among the groups for the presence of gross aggregates. In addition, in vitro tests done during storage showed platelets to be of similar quality for all resuspension methods. These findings suggest that any one of the protocols used for resuspension may be suitable for platelet preparation. The protocol that eliminates the rest period and does not require manual agitation is desirable for logistical reasons. This would simplify the preparation protocol as well as allow the availability of processed platelets sooner than if a 90 minute rest period was used. Our studies were confined to the assessment of in vitro properties of platelets during storage; studies of the in vivo hemostatic effectiveness of platelets resuspended by different methods are indicated. References 1. H a n d i n, R. I., F o r t i e r, N. L., and V a l e r i, C. R.: Platelet response to hypotonic stress after storage at 4 C or 22 C. Transfusion 10: , H o l m e, S., V aidja, K., and M u r p h y, S.: Platelet storage at 22 C: Effect of type of aggregation on morphology, viability and function in vitro. Rlood 52: , K akaiya, R. M., and K a t z, A. J.: Platelet preservation in large containers. Vox Sang, (in press). 4. K a t z, A. J., G e n c o, P. V., B l u m b e r c, N., S n y d e r, E. L., C a m p, B., and M o r s e, E. E.: Platelet collection and transfusion using the
5 37 0 KIRALY, BERNIER, KAKAIYA, AND CABLE Fenwal CS-3000 cell separator. Transfusion 10. Nie, N. H., H ull, C. H., Jenkins, J. G., Stein- 21: , brenner, K., and Bent, D. H.: Statistical 5. K unicki, R. J., T u c e l l i, M., B e c k e r, G., and Package for the Social Sciences, 2nd ed. New Aster, R. H.: A study of variables affecting the York, McGraw-Hill, quality of platelets stored at room temperature. Transfusion 15: , covery of platelets in platelet rich plasma by sim 11. R eiss, R. F. and K at z, A. J.: Optimizing re 6. M o u r a d, N.: A simple method for obtaining plex strategy. Transfusion 16: :1975. platelet concentrations free of aggregates. Transfusion 8:48, R o c k,g. and F i g u e r e d o,a.: Metabolic changes 7. M o u r a d, N.: Studies on release of certain enzymes from human platelets. Transfusion during platelet storage. Transfusion 16:17-19, 8 : , Sl i c h t e r, S. J., and H a r k e r, L. A.: Preparation 8. M iller, W. V : Technical Manual of the AABB, and storage of platelet concentrates. Transfusion 7th ed. Washington, American Association of i6:8-12, Blood Banks, 1974, pp Sn y d e r, E. L., H e z z e y, A., K a t z, A. J., and 9. M u r p h y, S., Sa y a r, S., and G a r d n e r, F.: Storage B o c k, J.: Occurrence of the release reaction of platelet concentrates at 22 C. Blood 35:549- during preparation and storage of platelet concentrates. Vox Sang. 41:172 77, 557,
C o r p o r a t e l i f e i n A n c i e n t I n d i a e x p r e s s e d i t s e l f
C H A P T E R I G E N E S I S A N D GROWTH OF G U IL D S C o r p o r a t e l i f e i n A n c i e n t I n d i a e x p r e s s e d i t s e l f i n a v a r i e t y o f f o r m s - s o c i a l, r e l i g i
More informationIgG blocking: An alternative red cell treatment method for phenotyping
IgG blocking: An alternative red cell treatment method for phenotyping SBB Student Research Barbara A Veal, BS, MT (ASCP) Gulf Coast School of Blood Bank Technology 1400 La Concha Lane Houston, Texas 77054-1802
More informationPlatelet and Granulocyte Pheresis
A N N A L S O F C L I N I C A L A N D L A B O R A T O R Y S C IE N C E, Vol. 8, No. 6 Copyright 1978, Institute for Clinical Science Platelet and Granulocyte Pheresis LEO N N. SUSSMAN, M.D. Blood Bank,
More informationF O R M T H R E E K enya C ertificate of Secondary E ducation
N a m e : A d m. N o...... D a t e : C la ss:.... 565/1 FO R M 3 B U S I N E S S S T U D I E S P A P E R 1 T I M E : 2 H O U R S T R I A L 6 2 0 1 8 FO R M 3 B U S I N E S S S T U D I E S P A P E R 1 T
More informationMAST ACADEMY OUTREACH. WOW (Weather on Wheels)
MAST ACADEMY OUTREACH ELEMENTARY PROGRAM Adventures Aboard WOW (Weather on Wheels) Post-Site Packet MAST Academy Maritime and Science Technology High School Miami-Dade County Public Schools Miami, Florida
More informationThe M echanism of Factor VIII Inactivation by H um an Antibodies
ANNALS O F CLINICAL A N D LABORATORY SC IE N C E, Vol. 15, No. 1 Copyright 1985, Institute for Clinical Science, Inc. The M echanism of Factor VIII Inactivation by H um an Antibodies II. The Effect of
More informationCypExpress 3A4 Catalyzed Conversion of Testosterone (TE) to 6β- Hydroxytestosterone (HT)
TM CASE STUDY CypExpress 3A4 Catalyzed Conversion of Testosterone (TE) to 6β- Hydroxytestosterone (HT) Shuvendu Das, 1 Enrique Martinez, 2 and Mani Subramanian 1 1 Center for Biocatalysis and Bioprocessing,
More informationHuman placenta lactogen, HPL ELISA kit
Human placenta lactogen, HPL ELISA kit Catalog No. E1442h (96 tests) Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE
More informationForm and content. Iowa Research Online. University of Iowa. Ann A Rahim Khan University of Iowa. Theses and Dissertations
University of Iowa Iowa Research Online Theses and Dissertations 1979 Form and content Ann A Rahim Khan University of Iowa Posted with permission of the author. This thesis is available at Iowa Research
More informationRat Advanced Glycation End Products(AGEs) ELISA Kit
Rat Advanced Glycation End Products(AGEs) ELISA Kit Catalog No. CSB-E09413r (96 T) This immunoassay kit allows for the in vitro quantitative determination of rat AGEs concentrations in serum, plasma and
More informationHuman placenta lactogen (HPL)ELISA Kit. MyBioSource.com. Catalog No. MBS (96T)
\ Human placenta lactogen (HPL)ELISA Kit Catalog No. MBS703864 (96T) This immunoassay kit allows for the in vitro quantitative determination of human HPL concentrations in serum, plasma. Expiration date
More informationRayBio Human Thyroid Peroxidase ELISA Kit
RayBio Human Thyroid Peroxidase ELISA Kit Catalog #: ELH-TPX User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100
More informationHuman Paraneoplastic pemphigus (PNP)antibody ELISA Kit. Catalog No. MBS (96 T)
Human Paraneoplastic pemphigus (PNP)antibody ELISA Kit Catalog No. MBS704248 (96 T) This immunoassay kit allows for the in vitro quantitative determination of human Paraneoplastic pemphigus(pnp)antibody
More informationHuman von Willebrand Factor cleavingprotease(vwf-cp) ELISA Kit. Catalog No. MBS (96T)
Human von Willebrand Factor cleavingprotease(vwf-cp) ELISA Kit Catalog No. MBS705447 (96T) This immunoassay kit allows for the in vitro quantitative determination of human vwf-cp concentrations in serum,
More informationDRG International Inc., USA Web:
This kit is intended for Research Use Only This kit is not intended for in vitro diagnostic use. INTENDED USE This Human Adiponectin (ACRP30) ELISA kit is used for the non-radioactive determination of
More informationHuman papillomavirus,hpv ELISA Kit
Human papillomavirus,hpv ELISA Kit Catalog No: E0787h 96 Tests Operating instructions www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE
More informationMAGBIO MAGBIO. cfkapture 21 Kit (3-5 ml) PROTOCOL Contents. Isolation kit for circulating cell-free DNA from stabilized plasma.
MAGBIO Liquid Biopsy Research - We are where you start cfkapture 21 Kit (3-5 ml) Manual Revision v1.2 Catalog Nos. CFK-D5-5ML, CFK-D50-5ML Isolation kit for circulating cell-free DNA from stabilized plasma
More informationcfkapture 21 Kit (3-5 ml) Isolation kit for circulating cell-free DNA from stabilized plasma PROTOCOL
MAGBIO Liquid Biopsy Research - We are where you start cfkapture 21 Kit (3-5 ml) Manual Revision v1.1 Catalog Nos. CFK-D5-5ML, CFK-D50-5ML Isolation kit for circulating cell-free DNA from stabilized plasma
More informationNov Julien Michel
Int roduct ion t o Free Ene rgy Calculat ions ov. 2005 Julien Michel Predict ing Binding Free Energies A A Recept or B B Recept or Can we predict if A or B will bind? Can we predict the stronger binder?
More informationA new ThermicSol product
A new ThermicSol product Double-Faced Thermo-Electric Solar-Panel TD/PV & Solar Tracker & Rotation Device An EU-patent protected product TP4-referens.pdf D o y o u w a n t to c o n v e rt it i n to G re
More informationHuman Plasmin/Antiplasmin Complex ELISA Kit
Human Plasmin/Antiplasmin Complex ELISA Kit Catalog No: IHPAPKT-COM Lot No: SAMPLE INTENDED USE Human plasmin/antiplasmin (PAP) complex assay is intended for the quantitative determination of the covalent
More informationRNA Transport. R preps R preps
RNA Transport R0527-00 5 preps R0527-01 50 preps July 2014 RNA Transport Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Protocol...4 Storage Procedure...4 Recovery Procedure...5
More informationHuman Coagulation Factor X Total Antigen ELISA Kit
Human Coagulation Factor X Total Antigen ELISA Kit Catalog No: IHFXKT-TOT Lot No: SAMPLE INTENDED USE This human coagulation Factor X antigen assay is intended for the quantitative determination of total
More informationInformation System Desig
n IT60105 Lecture 7 Unified Modeling Language Lecture #07 Unified Modeling Language Introduction to UML Applications of UML UML Definition Learning UML Things in UML Structural Things Behavioral Things
More informationcgmp ELISA Kit (Direct Competitive) Based on Monoclonal Anti-cGMP Antibody
(FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSIS!) cgmp ELISA Kit (Direct Competitive) Based on Monoclonal Anti-cGMP Antibody Catalog No: E-EL-DS02 96T This manual must be read attentively and
More informationCATALASE ACTIVITY ASSAY KIT KB test (96 well plate)
CATALASE ACTIVITY ASSAY KIT KB-03-012 100 test (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Sample preparation Pag. 4 Reagents preparation Pag. 6 Assay protocol Pag.
More informationManipulating the Anisotropic Structure of Gold Nanostars using Good s Buffers
SUPPLEMENTAL INFORMATION Manipulating the Anisotropic Structure of Gold Nanostars using Good s Buffers Kavita Chandra, Kayla S.B. Culver, Stephanie E. Werner, Raymond C. Lee, and Teri W. Odom,,* Department
More informationA L A BA M A L A W R E V IE W
A L A BA M A L A W R E V IE W Volume 52 Fall 2000 Number 1 B E F O R E D I S A B I L I T Y C I V I L R I G HT S : C I V I L W A R P E N S I O N S A N D TH E P O L I T I C S O F D I S A B I L I T Y I N
More informationThe Ability C ongress held at the Shoreham Hotel Decem ber 29 to 31, was a reco rd breaker for winter C ongresses.
The Ability C ongress held at the Shoreham Hotel Decem ber 29 to 31, was a reco rd breaker for winter C ongresses. Attended by m ore than 3 00 people, all seem ed delighted, with the lectu res and sem
More informationRayBio Nickel Magnetic Particles
RayBio Nickel Magnetic Particles Catalog #: 801-108 User Manual Last revised January 4 th, 2017 Caution: Extraordinarily useful information enclosed ISO 1348 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationSerotonin (Human) ELISA Kit
Serotonin (Human) ELISA Kit Catalog Number KA1151 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationFault Tolerant Computing CS 530DL
Fault Tolerant Computing CS 530DL Additional Lecture Notes Modeling Yashwant K. Malaiya Colorado State University March 8, 2017 1 Quantitative models Derived from first principles: Arguments are actual
More informationCanine Erythropoietin,EPO ELISA Kit
Canine Erythropoietin,EPO ELISA Kit Catalog No: E0028c 96 Tests Operating instructions www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE
More informationGENETIC ALGORlIHMS WITH AN APPLICATION TO NONLINEAR TRANSPORTATION PROBLEMS
101 GENETIC ALGORlIHMS WITH AN APPLICATION TO NONLINEAR TRANSPORTATION PROBLEMS MATTHEW HOBBS ISOR, VICTORIA UNIVERSITY WELLINGTON Abstract In this paper I will describe the theory and implementation of
More informationCase Study of Electronic Materials Packaging with Poor Metal Adhesion and the Process for Performing Root Cause Failure Analysis
Case Study of Electronic Materials Packaging with Poor Metal Adhesion and the Process for Performing Root Cause Failure Analysis Dr. E. A. Leone BACKGRUND ne trend in the electronic packaging industry
More informationHuman Coagulation Factor XII Total Antigen ELISA Kit
Human Coagulation Factor XII Total Antigen ELISA Kit Catalog No: IHFXIIKT-TOT Lot No: SAMPLE INTENDED USE This human coagulation Factor XII antigen assay is intended for the quantitative determination
More informationMAGBIO MAGBIO. cfkapture 21 Kit (1-2ml) PROTOCOL Contents. Isolation kit for circulating cell-free DNA from stabilized plasma.
MAGBIO Liquid Biopsy Research - We are where you start cfkapture 21 Kit (1-2ml) Manual Revision v1.1 Catalog Nos. CFK-D5-2ML, CFK-D50-2ML Isolation kit for circulating cell-free DNA from stabilized plasma
More informationRayBio Human IDUA ELISA Kit
RayBio Human IDUA ELISA Kit Catalog #: ELH-IDUA User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA
More informationNAD + /NADH Assay [Colorimetric]
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name NAD + /NADH Assay [Colorimetric] (Cat. #786 1539, 786 1540) think proteins! think G-Biosciences
More informationab alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit
ab108798 alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit Instructions for Use For the quantitative measurement of Human alpha 1 Antitrypsin (SERPINA1) in plasma and serum. This product is for research use
More informationHuman Mullerian Inhibiting Substance/Anti-Mullerian hormone (MIS/AMH)Elisa Kit
Human Mullerian Inhibiting Substance/Anti-Mullerian hormone (MIS/AMH)Elisa Kit Catalog No. CSB-E12756h (96T) This immunoassay kit allows for the in vitro quantitative determination of human MIS/AMH concentrations
More informationRayBio Human CA-125 ELISA Kit
RayBio Human CA-125 ELISA Kit Catalog #: ELH-CA125 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationRabbit IgA ELISA Kit
CATALOG NO: IRKTAH1169 Rabbit IgA ELISA Kit LOT NO: SAMPLE INTENDED USE The total Rabbit IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in rabbit biological
More informationMag-Bind Soil DNA Kit. M preps M preps M preps
Mag-Bind Soil DNA Kit M5635-00 5 preps M5635-01 50 preps M5635-02 200 preps January 2013 Mag-Bind Soil DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationMouse Cholecystokinin (CCK) ELISA Kit
Mouse Cholecystokinin (CCK) ELISA Kit Catalog No. CSB-E08115m (96T) This immunoassay kit allows for the in vitro quantitative determination of mouse CCK concentrations in serum, plasma and other biological
More informationab83360 Ammonia Assay Kit
Version 9 Last updated 7 February 2019 ab83360 Ammonia Assay Kit For the measurement of total ammonia and ammonium levels in various samples View kit datasheet: www.abcam.com/ab83360 (use www.abcam.cn/ab83360
More informationRUO. DRG Amyloid beta Auto-Ab (serum) As of 14 Dec rm (Vers. 1.1)
1 INTRODUCTION Intended Use The DRG Amyloid β Auto-Ab ELISA is an enzyme immunoassay for measurement of autoantibodies against Amyloid Beta (Aβ) in human serum For research use only. Not for use in diagnostic
More informationFish superoxide dismutase (SOD) ELISA Kit
Fish superoxide dismutase (SOD) ELISA Kit Catalog Number. CSB-E15929Fh For the quantitative determination of fish superoxide dismutase (SOD) concentrations in serum, plasma, tissue homogenates. This package
More informationNucView TM 488 Caspase-3 Assay Kit for Live Cells
NucView TM 488 Caspase-3 Assay Kit for Live Cells Catalog Number: 30029 (100-500 assays) Contact Information Address: Biotium, Inc. 3423 Investment Blvd. Suite 8 Hayward, CA 94545 USA Telephone: (510)
More informationU.S. Patent No. 9,051,563 and other pending patents. Ver
INSTRUCTION MANUAL Direct-zol 96 RNA Catalog Nos. R2054, R2055, R2056 & R2057 Highlights Quick, 96-well purification of high-quality (DNA-free) total RNA directly from TRIzol, TRI Reagent and all other
More informationBovine creatine kinase M-type (CKM) ELISA kit
Bovine creatine kinase M-type (CKM) ELISA kit Catalog Number. CSB-EL005459BO For the quantitative determination of bovine creatine kinase M-type(CKM) concentrations in serum, plasma, tissue homogenates.
More informationAdditional Materials Required (NOT PROVIDED): 200 µl; anti-ifn-ω monoclonal (murine) antibody Streptavidin-HRP* 150 µl
Protocol VeriKine TM Human Interferon Omega ELISA Kit Catalog No: 41395 Lot No: Expiration: Assay Range: 4.7-300 pg/ml Store all components at 2 8 C Please review the protocol in its entirety prior to
More informationRabbit leptin(lep) ELISA Kit
Rabbit leptin(lep) ELISA Kit Catalog Number. CSB-E06971Rb For the quantitative determination of rabbit leptin(lep) concentrations in serum, plasma, tissue homogenates. This package insert must be read
More informationRat lecithin cholesterol acyltransferase (LCAT) ELISA Kit
Rat lecithin cholesterol acyltransferase (LCAT) ELISA Kit Catalog Number. CSB-E14201r For the quantitative determination of rat lecithin cholesterol acyltransferase (LCAT) concentrations in serum, plasma,
More informationKIM-1 ELISA. For the quantitative determination of Kidney Injury Molecule in various biological samples.
KIM-1 ELISA For the quantitative determination of Kidney Injury Molecule in various biological samples. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 41-KIMHU-E01 Size: 96
More informationPorcine Factor-related Apoptosis(FAS) ELISA Kit
Porcine Factor-related Apoptosis(FAS) ELISA Kit Catalog No. CSB-E09369p (96T) This immunoassay kit allows for the in vitro quantitative determination of porcine FAS concentrations in serum, plasma and
More informationRat Prolactin ELISA Kit
Rat Prolactin ELISA Kit Catalog No: IRPRLKT Lot No: SAMPLE INTENDED USE This rat prolactin antigen assay is intended for the quantitative determination of prolactin antigen in rat plasma. For research
More informationHuman vitamin A (VA) ELISA
Human vitamin A (VA) ELISA For the quantitative determination of human VA in serum and plasma Cat. No. KU-113 For Research Use Only. Not for use in diagnostic procedures. Page 1 of 6 Rev. 13335113 INTENDED
More informationCTX-II (Human) ELISA Kit
CTX-II (Human) ELISA Kit Catalog Number KA1225 96 assays Version: 15 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationResolving immunohematology Case Studies
Lorena I. Aranda MLS(ASCP) CM SBB Assistant Director Immunohematology Reference Lab QualTex Laboratory San Antonio, TX/Atlanta, GA SEABB March 2014 Program Objectives Describe the appropriate immunohematological
More informationHuman anti-deoxyribonuclease B, anti-dnase B ELISA Kit
Human anti-deoxyribonuclease B, anti-dnase B ELISA Kit Catalog No: E0311h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ
More informationFor the quantitative determination of IgE in equine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.
IgE (Equine) ELISA For the quantitative determination of IgE in equine serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 41-IGEEQ-E01 Size: 96 wells Version:
More informationDesigning the Human Machine Interface of Innovative Emergency Handling Systems in Cars
Designing the Human Machine Interface of Innovative Emergency Handling Systems in Cars M anfred D A N G E L M A IE R * and P etra M A N G O L ** *Fraunhofer IAO, N obelstr. 12, D -70569 Stuttgart, **IA
More informationab83360 Ammonia Assay Kit
Version 8 Last updated 18 December 2017 ab83360 Ammonia Assay Kit For the measurement of total ammonia and ammonium levels in various samples This product is for research use only and is not intended for
More informationReal Gas Equation of State for Methane
Kaliningrad 2012 1. Commission Regulation (EC) No 692/2008 of 18 July 2008. http://ec.europa.eu/enterprise/sectors/automotive/environment/eurovi/index_ en. htm, p. 130. 2. Payne J. F. B., Skyrme G. Int.
More information2 Semester Final Exam Study Guide
Vista Murrieta High School Advanced Placem ent Biology G. Nicholas nd 2 Semester Final Exam Study Guide The following questions will potentially be on the final exam. Answer all questions in an outline
More informationRayBio Human LBP ELISA Kit
RayBio Human LBP ELISA Kit Catalog #: ELH-LBP User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA
More informationRayBio Human VEGFR1 ELISA Kit
RayBio Human VEGFR1 ELISA Kit Catalog #: ELH-VEGFR1 User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationHUMAN MYELOPEROXIDASE
HUMAN MYELOPEROXIDASE Immunoperoxidase Assay for Determination of Myeloperoxidase in Human Samples DIRECTIONS FOR USE Version3 GB0073 For Research Use Only, NOT for Diagnostic Purposes Please Read this
More informationHuman 17-Hydroxyprogesterone, 17-OHP ELISA kit
Human 17-Hydroxyprogesterone, 17-OHP ELISA kit Catalog No. E0454h (96 tests) Operating instruction USCNLIFE TM www.uscnlife.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE
More informationMouse Platelet Factor 4 (PF4) ELISA
Mouse Platelet Factor 4 (PF4) ELISA For the quantitative determination of mouse PF4 in plasma Cat. No. KT-25527 For Research Use Only. Not for use in diagnostic procedures. Page 1 of 7 Rev. 2010-02-17
More informationMicroCell User manual
MicroCell User manual In Vitro Diagnostics Do not re-use, discard after procedure Caution: Consult accompanying documents Catalog number Vitrolife Sweden AB, Box 9080 (Gustaf Werners Gata 2) SE-400 92
More informationMouse Tri-iodothyronine, T3 ELISA Kit
Mouse Tri-iodothyronine, T3 ELISA Kit Catalog No: E0453m 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE
More informationgenesig Easy q16 Validation kit
Primerdesign TM Ltd genesig Easy q16 Validation kit For use on the genesig q16 50 reactions For general laboratory and research use only. 1 genesig Easy: at a glance guide For each validation test Component
More informationCortisol (Horse) ELISA Kit
Cortisol (Horse) ELISA Kit Catalog Number KA2298 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationIrradiation Effect On Aging Red Blood Cells*
ANNALS OF CLINICAL AND LABORATORY SCIENCE, Vol. 21, No. 6 Copyright 1991, Institute for Clinical Science, Inc. Irradiation Effect On Aging Red Blood Cells* ELAINE K. JETER, M.D., RICHARD H. GADSDEN, Ph
More informationmrna Isolation Kit for Blood/Bone Marrow For isolation mrna from blood or bone marrow lysates Cat. No
For isolation mrna from blood or bone marrow lysates Cat. No. 1 934 333 Principle Starting material Application Time required Results Key advantages The purification of mrna requires two steps: 1. Cells
More informationExam: practice test 1 MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
Exam: practice test MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. Solve the problem. ) Using the information in the table on home sale prices in
More informationIgG (Rabbit) ELISA Kit
IgG (Rabbit) ELISA Kit Catalog Number KA2017 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationHydrogen Peroxide Colorimetric Detection Kit
Hydrogen Peroxide Colorimetric Detection Kit Catalog Number KA1017 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the
More informationcamp Direct Immunoassay Kit
camp Direct Immunoassay Kit Catalog Number KA0886 100 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationIgE (Rat) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only.
IgE (Rat) ELISA Kit Catalog Number KA1951 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General Information...
More informationTitle: Department: Previous Version(s): Replaces:
Title: Department: Pediatric Massive Transfusion Protocol (MTP) Trauma Services Effective Date: 09/2014 Reviewed: Policy and Protocol Previous Version(s): Replaces: **The reader is cautioned to refer to
More informationCatalase Fluorometric/Colorimetric Assay Kit
Catalase Fluorometric/Colorimetric Assay Kit Catalog No. KM0044 Detection and Quantification of Catalase Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic or Clinical
More informationIgE (Dog) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only.
IgE (Dog) ELISA Kit Catalog Number KA1937 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General Information...
More informationRayBio Human Semaphorin 6D ELISA Kit
RayBio Human Semaphorin 6D ELISA Kit Catalog #: ELH-SEMA6D User Manual Last revised February 9, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100
More informationP a g e 5 1 of R e p o r t P B 4 / 0 9
P a g e 5 1 of R e p o r t P B 4 / 0 9 J A R T a l s o c o n c l u d e d t h a t a l t h o u g h t h e i n t e n t o f N e l s o n s r e h a b i l i t a t i o n p l a n i s t o e n h a n c e c o n n e
More informationIsolation o f T Lymphocyte Subsets from Peripheral Blood Using Monoclonal A ntilym phocyte Antibodies*
ANNALS O F CLINICAL A N D LABORATORY SC IE N C E, Vol. 15, No. 1 Copyright 1985, Institute for Clinical Science, Inc. Isolation o f T Lymphocyte Subsets from Peripheral Blood Using Monoclonal A ntilym
More informationHuman small dense low density lipoprotein(sldl) ELISA Kit
Human small dense low density lipoprotein(sldl) ELISA Kit Catalog Number. For the quantitative determination of human small dense low density lipoprotein(sldl) concentrations in serum, plasma, saliva,
More informationRat Ferritin ELISA. For the determination of ferritin in serum and plasma of rats. For Research Use Only. Not For Use In Diagnostic Procedures.
Rat Ferritin ELISA For the determination of ferritin in serum and plasma of rats. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 41-FERRT-E01 Size: 96 Wells Version: 3 L52-16
More informationRayBio Human B7H1/PD-L1 ELISA Kit
RayBio Human B7H1/PD-L1 ELISA Kit Catalog #: ELH-B7H1 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationMouse Lipocalin-2/NGAL ELISA
K-ASSAY Mouse Lipocalin-2/NGAL ELISA For the quantitative determination of lipocalin-2/ngal in mouse biological samples Cat. No. KT-662 For Research Use Only. 1 Rev. 10747500 K-ASSAY PRODUCT INFORMATION
More informationMonkey Kidney injury molecule 1,Kim-1 ELISA Kit
Monkey Kidney injury molecule 1,Kim-1 ELISA Kit Catalog No: E0785Mo 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH
More informationHuman glycated hemoglobin A1c (GHbA1c) ELISA Kit
Human glycated hemoglobin A1c (GHbA1c) ELISA Kit Catalog Number. CSB-E08139h For the quantitative determination of human glycated hemoglobin A1c (GHbA1c) concentrations in lysate for RBC. This package
More informationDRG International, Inc., USA Fax: (908)
Please use only the valid version of the package insert provided with the kit. This kit is intended for Research Use Only. Not for use in diagnostic procedures. 1 INTENDED USE Competitive immunoenzymatic
More informationA Study of Drink Driving and Recidivism in the State of Victoria Australia, during the Fiscal Years 1992/ /96 (Inclusive)
A Study of Drink Driving and Recidivism in the State of Victia Australia, during the Fiscal Years 1992/93-1995/96 (Inclusive) M ichael M o l o n e y, D iane P a l a ia V ictia Police Fce, T raffic A lcohol
More informationEffects of Irradiation on Red Cells Stored in CPDA-1 and CPD-ADSOL (AS-1)*
ANNALS OF CLINICAL AND LABORATORY SCIENCE, Vol. 21, No. 3 Copyright 1991, Institute for Clinical Science, Inc. Effects of Irradiation on Red Cells Stored in CPDA-1 and CPD-ADSOL (AS-1)* ELAINE K. JETER,
More informationHuman arachidonic acid (AA) ELISA Kit
Human arachidonic acid (AA) ELISA Kit Catalog Number. CSB-E09040h For the quantitative determination of endogenic human arachidonic acid (AA) concentrations in serum, plasma, cell culture supernates, tissue
More informationImmunoassay Kit (Colorimetric)
RayBio cgmp Direct Immunoassay Kit (Colorimetric) User Manual Version 1.0 May 25, 2014 RayBio cgmp Direct Immunoassay Kit (Colorimetric) Protocol (Cat#: 68AT-cGMP-S100) RayBiotech, Inc. We Provide You
More informationNitrate Reductase (NR) Colorimetric Assay Kit
(FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSIS!) Nitrate Reductase (NR) Colorimetric Assay Kit Catalog No: E-BC-K158 Method: Colorimetric method Specification: 100 Assays This manual must
More information