Snork Synthesis Lab Lab Directions

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1 Snork Synthesis Lab Lab Directions This activity, modified from the original at The Biology Corner, will help you practice your understanding of protein synthesis. Submit your lab answers according to the directions and grading rubric below. Overview: I n t his si m u l ati o n, y o u w ill e x a m i n e t h e D N A s e q u e n c e of a fi ctiti o u s o r g a n i s m, t h e S n o r k. S n or k s w e r e di s c o v e r e d o n t h e pl a n e t D e e E n a e i n a di s t a n t s ol ar s y s t e m. S n o r k s o n l y h a v e one chromosome with ten genes on it. Y o u r j o b i s t o a n aly z e t h e g e n e s of its D N A a n d d et er m i n e w h a t tr ait s t h e o r g a n i s m h a s a n d t h e n c r e at e a s k et c h of t h e or g a n i s m ( Y o u c a n b e c r e ati v e h er e). Goal: Transcribe and translate all 10 genes for one snork. Then, draw a picture of your snork based on your work. Directions: F o r s i m plicit y, t h e g e n e s e q u e n c e s ar e m u c h s m a l l er t h a n real gene s e q u e n c e s f o u n d i n li vi n g o r g a n i s m s. E a c h g e n e h a s t w o p o s s i bl e v er si o n s t h at r e s u lt i n a diff er e n t tr ait b ei n g e x pr e s s e d i n t h e s n or k. Example: the gene for legs can result in either 2 or 4 legs Snork Genes Amino acid sequence (abbreviations used) Trait expressed (outward appearance) 1 Body Hair val - ser - leu val - ser - lys 2 Body Style tyr - pro - glu - glu - lys val - pro - thr - glu - lys 3 Legs leu - leu - leu - pro leu - leu - ser - ala 4 Head Shape ala - val - val val - ala - ala 5 Tail his - ile his - his hairless hairy plump skinny 2 legs 4 legs round head square head tail no tail S n o r k S y n t h e s i s L a b 1 of 5

2 6 Body Pigment ser - pro - val val - phe - tyr 7 Eyes asp - ile - leu - leu - pro - thr asp - ile - pro - pro - pro - thr 8 Mouth val - asp - asp - ala asp - asp - asp - ala 9 Ears phe - ser - gly phe - phe - gly 10 Arms arg - tyr - cys - lys arg - arg - asp - thr blue pigment (hair/skin) red pigment (hair/skin) small slanted eyes large round eyes circular mouth rectangular mouth pointed standing-up ears rounded floppy ears long spaghetti like arms short stumpy arms 1. C h o o s e a s n or k b el o w ( d o n ot c h o o s e o u r e x a m p l e, S n o o p y. ) 2. Transcribe th e D N A i nt o m R N A. 3. Translate t h e m R N A giv e n i nt o a n a m i n o a c i d s e q u e n c e t o d et er m i n e t h e tr ait ( h o w t h e o r g a n i s m l o o k s ) b a s e d o n t h e s e q u e n c e. ( U se the codon wheel or table given to you in the content to do this step.) 4. D r a w y o u r S n or k! First, let s try an example. You can see Snoopy s DNA below. Each gene is in order (1-10) and is separated by a slash to keep you organized. Snoopy Snork DNA: C A T A G G G A G / A T G G G G C T C C T T T T T / A A T A A T G A C G G G / C G A C A A C A T / G T A T A A / A G A G G G C A T / C T A T A A G A A G A C G G G T G T / C A A C T A C T A C G G / A A A A G A C C A / G C G G C A C T G Transcribe the DNA into mrna. Remember that there are no T s in mrna, use U s instead. Example: if the DNA codon reads CAT, the matching mrna codon is GUA. Snoopy Snork mrna: G U A U C C C U C / U A C C C C G A G G A A A A A / U U A U U A C U G C C C / G C U G U U G U A / C A U A U U / U C U C C C G U A / G A U A U U C U U C U G C C C A C A / G U U G A U G A U G C C / U U U U C U G G U / C G C C G U G A C Now, use your codon wheel or table to find the amino acid that matches each codon. *Note that abbreviations were used for amino acids.* (This table is enlarged below for your reference.) Write down the amino acids in order for each gene, as shown. Then compare each protein (amino acid chain) to the trait table above to find out the appearance of your Snork! S n o r k S y n t h e s i s L a b 2 of 5

3 Amino Acid Sequence: 1 : v al, s e r, l e u ( = h airl es s i n t a bl e a b o v e ) 2 : t y r, pr o, glu, gl u, l y s ( = pl u m p ) 3 : l e u, l e u, l e u, pr o ( = 2 l e g s ) 4 : al a, v al, v al ( = r o u n d h e a d ) 5 : h i s, il e ( = t ail) 6 : s e r, pr o, v al ( = blu e ) 7 : a s p, il e, l e u, l e u, pr o, t h r ( = s m all, sl a n t e d e y e s ) 8 : v al, a s p, a s p, al a ( = ci r c ul ar m o u t h ) 9 : p h e, s e r, gly ( = p oi nt e d e ar s ) 1 0 : ar g, ar g, a s p ( = s h or t ar m s ) Now draw your Snork using the information you translated! (Sample drawing courtesy of a young biologist!) N o w, y o u will c h o o s e o n e of t h e f oll o w i n g S n o r k s a n d d o t h e s a m e t h i n g! Snicker Snork DNA: C A G T C G G A A / C A A G G G T G T C T T T T T / G A G A A T T C A C G C / C A A C G C C G A / G T G T A A / A G A G G G C A T / C T A T A A G G G G G G G G G T G G / C T A C T G C T G C G T / A A G A A A C C C / G C G G C C C T G Snuffle Snork DNA: C A T A G A T T T / C A A G G A T G A C T T T T C / G A A G A G G A G G G G / C A A C G C C G A / G T A G T G / C A T A A A A T A / C A T T A A G A A G A C G G G T G T / C A A C T G C T G C G T / A A G A G C C C A / T C T A T A A C A Snapple Snork DNA: S n o r k S y n t h e s i s L a b 3 of 5

4 C A G T C G T T T / A T G G G G C T T C T C T T T / G A G A A T T C A C G C / C G A C A A C A C / G T A G T A / C A A A A A A T G / C T A T A G A A T G A C G G G T G G / C T G C T G C T A C G G / A A A A G A C C C / T C T A T A A C A Be sure to double check your work so that you do not make any silly mistakes! mrna Table with Amino Acid abbreviations S n o r k S y n t h e s i s L a b 4 of 5

5 Data/Results 1. W h i c h s n or k di d y o u s el e c t f or t his a c ti vit y? 2. W h at w a s it s m R N A n u c l e oti d e s e q u e n c e? P l e a s e i n cl u d e s p a c e s a n d sl a s h e s a s y o u s e e i n D N A. 3. W h at w a s it s a m i n o a c i d s e q u e n c e? I n cl u d e s l a s h e s or w rit e e a c h of 1 0 c h ar a c t eri s ti c s o n s e p a r at e li n e s. 4. I n cl u d e a n ori g i n al pi c t u r e of y o u r s n or k wi t h t h e 1 0 l a b el e d c h ar a c t eri s ti c s. Y o u m a y h a n d - d r a w y o u r s n or k a n d t a k e a pic t u r e/ s c a n it i n, or y o u c a n c r e at e it u s i n g a dr a w i n g p r o g r a m ( s e e t h e li st of o pti o n s i n t h e s i d e b a r i n t h e c o n t e n t. ) From sis_shared6.html S n o r k S y n t h e s i s L a b 5 of 5

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