Modified Nucleotides of Mitochondrial Transfer Ribonucleic Acids

Transcription

1 The College at Brockport: State University of New York Digital Environmental Science an Ecology Theses Environmental Science an Ecology 1973 Moifie Nucleoties of Mitochonrial Transfer Ribonucleic Acis Davi William Morry The College at Brockport, Follow this an aitional works at: Part of the Biology Commons Repository Citation Morry, Davi William, "Moifie Nucleoties of Mitochonrial Transfer Ribonucleic Acis" (1973). Environmental Science an Ecology Theses This Thesis is brought to you for free an open access by the Environmental Science an Ecology at Digital It has been accepte for inclusion in Environmental Science an Ecology Theses by an authorize aministrator of Digital For more information, please contact

2

3 "No information erive from this thesis may be publishe without permission of the original author, with whom copyright lies."

4 I su P. are ). a summer a

5 t o on nuc-

6 ac N (or ) we

7 I..1 II. Ill Ill 4 I.. It Ill Ill It "' Ill v. Ill Iii Ill

8 1 ion, at are not coincient o that (such as the tite } were in a non-menelian way. that ce some amount tic which locate e re ome s the years following not only their own se are ne;.;c :::> c:t.r. a e c are

9 2 are can mitochonria tem t from that mitochonrial genome probably not contain sufficient DNA to e Gf this istinct mitochonrial protein aynthes Some of the necessary compo nents may be nuo coe transporte.into the mitochonria. For the total ce to function e, mitochonrion must coorin.ate with the act the nuo an the cell as a whole. Many have shewn that the mitochonrial v n trna seems ce a 1 the cytopla.sm.that co- ae as a the s

10 re c of the ce this,problem in the question of th e mi tochenrial trna, biosynthes, structure function. known that the mitochonrial in the elution methylate on trna. the, of ic ac

11 4 an r 2 or )' 1. rre. c ). same ac a r or c are or are as nuc l cursor r a

12 5 trna CH2 A 0 OH R -- CH c II

13 6 we 2 some s, 2 some ome ac assume a a r- cases occurs

14 0 HN A i 2 N. HOH2C 0 N N> ribose HN NH ribose 4 - thiouraci I pse uouroc il HO OCH3 2 '- 0- me thyl guanosine HI' H CH \ I 3 c-==c \, ch2 CH3 2 N 6 --(A -isopentenyl) enosine N N I 0 I H c - o-c 3 'NH H3c - o-...c / --He // \ fluorescen t base " Y " 0 H2C/ CH2 ) H3C ) r ibose 0 I N N I ribose m 0 0 a "0

15 8 3'en A-C>H I c ' c 89 G am ino aci acceptor arm pseuouriine loo p I G A au. C --- 4JII' \ /,_----, hl..._g.--a 26 I ',.,- N ''- \ 7'6 / P y ',.. C A \ ) Pu ihyrou r iine loo p ( u P y / IN \ hy permoifie nuc Ieos ie an ticoon

16 9 l. s 6 7 en l are some s are on ao 9

17 some cases nuc omes. nuc os on on a ce ce some cases own s a ce on an- anae ce

18 ce 0 s most rns sea s an ( as et e ce or ranees mouse

19 ce mouse a r re se source s are or s are cursors or s are SOllle

20 no se c own a some.

21 serum X ee were me as we as serum as were s were me

22 l, s r were s ( s ) we re or as r we re me ) re cove re X ( 5 sus-

23 ce X g to remove nuc t.,000 X g from at the same t. times re- sucrose (0. M, 7.5, M f X t then 1 t use as source 4 0,1 M one O.D. were an re one 0,1 M ous

24 1. 5 em). were e (0,5 X as 5 X we a ce ( 2.5 X ee ) e ous (OCM5 X 1.5 om ) (0.1 N on remove a e we re we re (lcl)

25 were s o. were were 5 rs) a mens : ) ' ene on were s, were

26 were ass nuc on

27 we a (see nuc one case case as as s c s. r nuc r nuc s t me se ac on en

28 21 r

29 nuc o- r o- ce a on"' are re s were or c were summe 2. se one are

30 1 r * 2. J, 2, 4,, ). 2, 3. J f 4. J, 7, ), 8, 2,, 5. ' ' f, 2, ', 6. J, 5. 2, 6, ' 7- ), 6., ),?, 2, ' fill ), 1, 4,, , 1, 1, 6, 2, ), J. s, 1,, 1, 1, 1,,,

31 2 nuc 1., 2. 1, 4, , 4. 2, 2, 2, 1, 8, 5. 2, 1, 6, 6. l, l, 4, 7. 2, 2, 2, 2, ' a. 1,, 1, 7,

32 J 4 as c ac as a ac were are 0,

33 J o , o. o. o. ). o. o. o. o. 0, o o. 0, o. 5. o o. o. o , 2, o. 3 o. o o. o. o. 8, o. o. o , o. o. o. ill o. 1. 1, o. 0, o. 1, 1. o. o , 2, o. o o o. o. av, l. l. 1. o. o. o. * nuc -y;

34 o. o. o. o. o. o. ) o. o. o. o o. o. o. o. o. s o. o. o. o. 6. o. 1. o. o. o. o. 7. l. o. o. o. o. a. 1. l, l. o. o. o. av. 1. l. o. o. o. o.

35 a o- a same ce some as con- on eons cause are 1 nuo 'S

36 5 o- 4.

37 o- a as re case. a must

38 some are can nuc s or (2) re- ) some an- en

39 , some en- as we 3 no s a own somes, as s a come

40 re nuc

41

42 ( ) c. ' ( ) '., ) ) ) ( )

43