1901 Application of Spectrophotometry
|
|
- Ellen Watts
- 5 years ago
- Views:
Transcription
1 1901 Application of Spectrophotometry Chemical Analysis Problem: 1 Application of Spectroscopy
2 Organic Compounds Organic compounds with single bonds absorb in the UV region because electrons from single bonds are tightly held. Electrons in double or triple bonds absorb in the UV and visible region are called chromophores. Chromophores are unsaturated organic functional groups that red shifts if conjugation of two or more chromophores are in a compound. 2 Application of Spectroscopy
3 Inorganic Compounds Inorganic compounds involve transition between d-orbitials. Crystal field theory explains the nature of the transition. Absorption spectra of ion of the lanthanide and actinide series differ substantially from transition metals. 3 Application of Spectroscopy
4 Charge Transfer Transitions An important class of absorption is the chargetransfer which originates from electron-donor group bonded to an electron acceptor group. The excited state results in an internal oxidation/reduction process. Examples are phenolic complex of iron, pyridyl complexes, the 1,10-phenanthroline complexes, the iodide complex of molecular iodine and the ferro/ferricyanide complex. Some of these transition originate from metal to ligand charge transfer and others are ligand to metal charge transfer. In some charge transfer process, the excited complex dissociate and produce photochemical oxidation/reduction products. Iridium bipyridine complexes 4 Application of Spectroscopy
5 UV-Vis Spectral Limits UV-Vis spectra do not have sufficient fine structure to permit unambiguous identification. UV-Vis must be supplemented with other physical or chemical evidence. For qualitative analysis, it is important that the solvent is transparent in the region of the chromophores. The table shows the window for various solvents. Effect of slit width. The spectra shows cytochrome C under four spectral bandwidth (1) 20 nm, (2) 10 nm, (3) 5 nm, and (4) 1 nm. Scattering should also be corrected for. In some instances, false peak will be observed because of operation of the spectrometer to its wavelength extreme. Shown is the spectra of cerium(iv) obtained in glass optical (A) and in quartz (B). 5 Application of Spectroscopy
6 Versatility of UV-Vis spectroscopy Most important characteristics of spectrophotometric and photometric methods are: 1. Wide applicability - Inorganic, organic and biochemicals can be analyzed using this technique. 90% of analysis in lab are based on UV-Vis spectroscopy. 2. High Sensitivity - Analysis can be performed with dilute solutions, 10-4 to 10-5 M and at times 10-7 M 3. Moderately high selectivity - Can perform analysis without interference of solvent or other chemicals. 4. Good accuracy - The relative error in the technique is in the order of 1% - 5% which can be improved to 0.1 % 5. Ease of convenience - One of the easiest technique to use with minimal setup. The analysis can also be automated. 6 Application of Spectroscopy
7 Application to Absorbing and nonabsorbing species Absorbing Species - Ideal technique if the chemical being analyzed absorbs in region that can be detected. Nonabsorbing speices - Chemicals that do not absorb, can be reacted with chromophoric reagents to yield products that can be analyze using UV-Vis spectroscopy. Typical chelating reagent for absorption (a) diethyldithicarbamante (b) Diphenylthiocarbazone 7 Application of Spectroscopy
8 Procedural Details Things to consider to yield reliable results in absorption spectroscopy: Wavelength selector - Maximum sensitivity yield good results if wavelength is used in which the absorbance is the largest. Absorbances factors - Factors such as solvent, ph, temperature, ionic strength... must be controlled to minimize complication of spectra. Concentration relationship - Standardization plots must match as close as possible the condition of chemical being analyzed. Standard addition - Matrix effect of can be minimized by introducing into the standards species that approximate the amounts found in the sample via standard addition. Analysis of mixture - Total absorbance features overlap linearly for two or more components. In principle, the components can be separated to their individual components. 8 Application of Spectroscopy
9 Instrumental Uncertainties Accuracy and precision of spectrophotometric analysis limited by indeterminate errors. These errors are summarized in the following table. A B C Error in the measurements of T (transmittance) is constant and independent of the magnitude of T. The type of instrument may give rise to error in the meter read out. Above are graphs showing the error curves for various categories of instrument uncertainties. 9 Application of Spectroscopy
10 Error in Transmittance for Different Instruments The RSD analysis for the two instruments show that absorbance lower than 0.1 are not reliable and should be avoided. The reason is the concentration is directly proportional to the difference in intensity of Po and Pi, through A = log Po -log Pi. At low concentrations, log P is nearly large as Po and a small difference in large numbers lead to big errors. Note as well that at absorbance higher than 1.2 is also not reliable and that is because the power of the beam is so low that it cannot be measured accurately. Experiment curves relating relative concentrations uncertainties to absorbance for two spectrometers. Data obtained with (a) Spectronic 20 and (b) a Cary 118 spectrophotometer. 10 Application of Spectroscopy
11 Photometric and Spectrophotometric Titrations Equivalent points in titration can be determine by spectrophotometric analysis. Analysis requires that one or more of the reactant or product shows absorbance features. Molar absorptivites of the analyte titrated, the product, and the titrant are e A, e P and e T respectively. Photometric titration curve at 745 nm to 100mL of a solution that was M in Bi 3+ and Cu Application of Spectroscopy
12 Analysis of Complex ions Composition of a complex in solution can be determined without actually isolating the complex as a pure compound. The techniques used for such studies are (1) method of continuous variations, (2) the mole-ratio method, and (3) the slope-ratio method. Continuous variation: Method in which the cation and ligand in solutions have identical analytical concentrations with total volume and moles of reactant constant but mole ratio of reactants systematically varied. In the graph shown, the curve maxima is the result of incompleteness of the complexformation reaction. Mole-ratio: Method in which analytical concentration of one reactant (usually cation) is held constant with variation of other reactant. Slope-ratio : Method linear regression is used with Beer s Law. The method assumes (1) complex-formation reaction is complete with excess of either reactant. (2) Beer s Law is obeyed. (3) Complex absorbs at desired wavelength. 12 Application of Spectroscopy
13 Infrared Spectroscopy Versatile technique that provides molecular structure information. This makes the technique optimal for qualitative analyses but poor for quantitative analyses. The energy of infrared can excite vibration and rotational transitions, but are not sufficient for electronic transitions. Samples in the solid, liquid and gas phase can be analyzed. 13 Application of Spectroscopy
14 IR Instrumentation There are three types of IR instruments: dispersive spectrometers, Fourier-Transformed (FTIR), and filter photometers. The first two provide complete spectral analysis with the filter photometer designed for quantitative analysis. Filter Photometers: General use to monitor air particulates and can measure amounts of particles in the gas phase. Dispersive Instruments: Double beam in which samples are placed between light source and monochromator. The radiation source is generally thermal with the detectors sensitive to the heat rather hand photons. Fourier Transformed: High speed with great resolution because all wavelengths are detected and measured simultaneously using a Michelson interferometer. The basic idea of this techniques is that the source signal is modulated and passed through the source sample. The resulting signal is an interferogram that is Fourier Transformed to yield all the frequencies that was absorbed by the sample. 14 Application of Spectroscopy
15 Spectra Interpretation IR frequencies for various functional groups 15 Application of Spectroscopy
16 Quantitative Applications of IR IR spectra consist of bands that correspond to infrared absorption. Bands in the shorter wavelength, mm ( wavenumbers, cm -1 ), are characteristics of functional groups in the structure of the chemical. To truly identify chemical the whole spectrum mm must be analyzed. It may be possible to compare the spectrum to published results in the literature Application of Spectroscopy
Chapter 13 An Introduction to Ultraviolet/Visible Molecular Absorption Spectrometry
Chapter 13 An Introduction to Ultraviolet/Visible Molecular Absorption Spectrometry 13A Measurement Of Transmittance and Absorbance Absorption measurements based upon ultraviolet and visible radiation
More informationSpectroscopy. Page 1 of 8 L.Pillay (2012)
Spectroscopy Electromagnetic radiation is widely used in analytical chemistry. The identification and quantification of samples using electromagnetic radiation (light) is called spectroscopy. Light has
More informationlevels. The signal is either absorbance vibrational and rotational energy levels or percent transmittance of the analyte
1 In this chapter, absorption by molecules, rather than atoms, is considered. Absorption in the ultraviolet and visible regions occurs due to electronic transitions from the ground state to excited state.
More informationSpectrophotometry. Introduction
Spectrophotometry Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle
More information9/28/10. Visible and Ultraviolet Molecular Spectroscopy - (S-H-C Chapters 13-14) Valence Electronic Structure. n σ* transitions
Visible and Ultraviolet Molecular Spectroscopy - (S-H-C Chapters 13-14) Electromagnetic Spectrum - Molecular transitions Widely used in chemistry. Perhaps the most widely used in Biological Chemistry.
More information09/05/40 MOLECULAR ABSORPTION METHODS
MOLECULAR ABSORPTION METHODS Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of wavelength ( absorption spectrum ), due to its interaction
More informationUltraviolet-Visible Spectroscopy
Ultraviolet-Visible Spectroscopy Introduction to UV-Visible Absorption spectroscopy from 160 nm to 780 nm Measurement of transmittance Conversion to absorbance * A=-logT=εbc Measurement of transmittance
More informationAdvanced Pharmaceutical Analysis
Lecture 2 Advanced Pharmaceutical Analysis IR spectroscopy Dr. Baraa Ramzi Infrared Spectroscopy It is a powerful tool for identifying pure organic and inorganic compounds. Every molecular compound has
More informationChem 310 rd. 3 Homework Set Answers
-1- Chem 310 rd 3 Homework Set Answers 1. A double line labeled S 0 represents the _ground electronic_ state and the _ground vibrational_ state of a molecule in an excitation state diagram. Light absorption
More informationAdvanced Analytical Chemistry
84.514 Advanced Analytical Chemistry Part III Molecular Spectroscopy (continued) Website http://faculty.uml.edu/david_ryan/84.514 http://www.cem.msu.edu/~reusch/virtualtext/ Spectrpy/UV-Vis/spectrum.htm
More informationCourse: M.Sc (Chemistry) Analytical Chemistry Unit: III
Course: M.Sc (Chemistry) Analytical Chemistry Unit: III Syllabus: Principle of spectrophotometry Types of spectrophotometer Applications - Dissociation constants of an indicator simultaneous spectrophotometric
More informationReference literature. (See: CHEM 2470 notes, Module 8 Textbook 6th ed., Chapters )
September 17, 2018 Reference literature (See: CHEM 2470 notes, Module 8 Textbook 6th ed., Chapters 13-14 ) Reference.: https://slideplayer.com/slide/8354408/ Spectroscopy Usual Wavelength Type of Quantum
More informationUltraviolet-Visible and Infrared Spectrophotometry
Ultraviolet-Visible and Infrared Spectrophotometry Ahmad Aqel Ifseisi Assistant Professor of Analytical Chemistry College of Science, Department of Chemistry King Saud University P.O. Box 2455 Riyadh 11451
More informationSpectroscopy Primer. for ultraviolet and visible absorbance spectroscopy. by Stephanie Myers Summer 2015
Spectroscopy Primer for ultraviolet and visible absorbance spectroscopy by Stephanie Myers Summer 2015 Abstract: An overview of uv vis absorbance spectroscopy including Beer s Law, calibration curves,
More informationUltraviolet-Visible and Infrared Spectrophotometry
Ultraviolet-Visible and Infrared Spectrophotometry Ahmad Aqel Ifseisi Assistant Professor of Analytical Chemistry College of Science, Department of Chemistry King Saud University P.O. Box 2455 Riyadh 11451
More informationReflection = EM strikes a boundary between two media differing in η and bounces back
Reflection = EM strikes a boundary between two media differing in η and bounces back Incident ray θ 1 θ 2 Reflected ray Medium 1 (air) η = 1.00 Medium 2 (glass) η = 1.50 Specular reflection = situation
More information1 WHAT IS SPECTROSCOPY?
1 WHAT IS SPECTROSCOPY? 1.1 The Nature Of Electromagnetic Radiation Anyone who has been sunburnt will know that light packs a punch: in scientific terms, it contains considerable amounts of energy. All
More informationAn Introduction to Ultraviolet-Visible Molecular Spectrometry (Chapter 13)
An Introduction to Ultraviolet-Visible Molecular Spectrometry (Chapter 13) Beer s Law: A = -log T = -logp 0 / P = e x b x C See Table 13-1 for terms. In measuring absorbance or transmittance, one should
More informationChapter 4 Ultraviolet and visible spectroscopy Molecular Spectrophotometry
Chapter 4 Ultraviolet and visible spectroscopy Molecular Spectrophotometry Properties of light Electromagnetic radiation and electromagnetic spectrum Absorption of light Beer s law Limitation of Beer s
More informationAnswers to spectroscopy questions. 1. Consider the spectrum below. Questions a f refer to this spectrum.
Answers to spectroscopy questions. 1. Consider the spectrum below. Questions a f refer to this spectrum. a. Is the spectrum above a band spectrum or a line spectrum? This is a band spectra, there are what
More informationChem Homework Set Answers
Chem 310 th 4 Homework Set Answers 1. Cyclohexanone has a strong infrared absorption peak at a wavelength of 5.86 µm. (a) Convert the wavelength to wavenumber.!6!1 8* = 1/8 = (1/5.86 µm)(1 µm/10 m)(1 m/100
More informationMOLECULAR ABSORPTION METHODS
MOLECULAR ABSORPTION METHODS Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of wavelength ( absorption spectrum ), due to its interaction
More informationLab #12: Determination of a Chemical Equilibrium Constant
Lab #12: Determination of a Chemical Equilibrium Constant Objectives: 1. Determine the equilibrium constant of the formation of the thiocyanatoiron (III) ions. 2. Understand the application of using a
More informationVisible and IR Absorption Spectroscopy. Andrew Rouff and Kyle Chau
Visible and IR Absorption Spectroscopy Andrew Rouff and Kyle Chau The Basics wavelength= (λ) original intensity= Ι o sample slab thickness= dl Final intensity= I f ε = molar extinction coefficient -di=
More information2001 Spectrometers. Instrument Machinery. Movies from this presentation can be access at
2001 Spectrometers Instrument Machinery Movies from this presentation can be access at http://www.shsu.edu/~chm_tgc/sounds/sound.html Chp20: 1 Optical Instruments Instrument Components Components of various
More informationChapter 17: Fundamentals of Spectrophotometry
Chapter 17: Fundamentals of Spectrophotometry Spectroscopy: the science that deals with interactions of matter with electromagnetic radiation or other forms energy acoustic waves, beams of particles such
More informationCHEM*3440. Photon Energy Units. Spectrum of Electromagnetic Radiation. Chemical Instrumentation. Spectroscopic Experimental Concept.
Spectrum of Electromagnetic Radiation Electromagnetic radiation is light. Different energy light interacts with different motions in molecules. CHEM*344 Chemical Instrumentation Topic 7 Spectrometry Radiofrequency
More informationChemistry Instrumental Analysis Lecture 15. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 15 IR Instruments Types of Instrumentation Dispersive Spectrophotometers (gratings) Fourier transform spectrometers (interferometer) Single beam Double beam
More informationPAPER No. 12: ORGANIC SPECTROSCOPY MODULE No. 7: Instrumentation for IR spectroscopy
KNOW MORE Web links https://en.wikipedia.org/wiki/infrared_ http://hiq.lindegas.com/en/analytical_methods/infrared_/non_dispersive_infrared.html http://blamp.sites.truman.edu/files/2012/11/322-ir-and-ftir.pdf
More informationCompact Knowledge: Absorbance Spectrophotometry. Flexible. Reliable. Personal.
L A B O R A T O R Y C O M P E T E N C E Compact Knowledge: Absorbance Spectrophotometry Flexible. Reliable. Personal. The interaction of light with molecules is an essential and well accepted technique
More informationApplication of IR Raman Spectroscopy
Application of IR Raman Spectroscopy 3 IR regions Structure and Functional Group Absorption IR Reflection IR Photoacoustic IR IR Emission Micro 10-1 Mid-IR Mid-IR absorption Samples Placed in cell (salt)
More informationDetermining Riboflavin Content in a Multivitamin: Things to do before the lab
Determining Riboflavin Content in a Multivitamin: Things to do before the lab Calculate everything you can that you can in advance Step 1: Calculate the dilution of a 4.0 M solution to 1 L of a 0.02 M
More information10/2/2008. hc λ. νλ =c. proportional to frequency. Energy is inversely proportional to wavelength And is directly proportional to wavenumber
CH217 Fundamentals of Analytical Chemistry Module Leader: Dr. Alison Willows Electromagnetic spectrum Properties of electromagnetic radiation Many properties of electromagnetic radiation can be described
More informationPAPER No. 12: ORGANIC SPECTROSCOPY MODULE No. 4: Basic principles and Instrumentation for IR spectroscopy
Subject Chemistry Paper No and Title Module No and Title Module Tag Paper 12: Organic Spectroscopy Module 4: Basic principles and Instrumentation for IR spectroscopy CHE_P12_M4_e-Text TABLE OF CONTENTS
More informationConcepts, Techniques. Concepts, Techniques 9/11/2012. & Beer s Law. For a simple, transparent, COLORED material, e.g. ROYGBV
9//22 OBJECTIVES Spectrophotometry of Food Dyes & Beer s Law Last Update: 9//22 9:54 AM What is the quantitative basis for the color of substances? How is the absorption/transmission of light measured?
More informationCh 313 FINAL EXAM OUTLINE Spring 2010
Ch 313 FINAL EXAM OUTLINE Spring 2010 NOTE: Use this outline at your own risk sometimes a topic is omitted that you are still responsible for. It is meant to be a study aid and is not meant to be a replacement
More informationUV-Vis Absorption Experiment 5: Beer- Lambert Law and the Temperature Dependence of the Crystal Violet- Sodium Hydroxide Reaction
1 UV-Vis Absorption Experiment 5: Beer- Lambert Law and the Temperature Dependence of the Crystal Violet- Sodium Hydroxide Reaction Overview In Part A of this experiment, the absorption behaviour of crystal
More informationPaper: 12, Organic Spectroscopy Module: 5, Applications of UV spectroscopy
Subject Chemistry Paper No and Title Module No and Title Module Tag Paper 12: Organic Spectroscopy Applications of UV-visible Spectroscopy CHE_P12_M5 TABLE OF CONTENTS 1. Learning Outcomes 2. Introduction
More informationChem 155 Quiz 3 Review Topics: Quiz 3 outline
Quiz 3 outline 1. Atomic absorption spectrometry a. Principles of FAAS where selectivity and sensitivity arise b. Spectrometer design c. Atomization processes d. Sensitvitiy and atomization: protecting
More informationChemistry Instrumental Analysis Lecture 11. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 11 Molar Absorptivities Range 0 to 10 5 Magnitude of e depends on capture cross section of the species and probability of the energy-absorbing transition. e
More informationQuestions on Instrumental Methods of Analysis
Questions on Instrumental Methods of Analysis 1. Which one of the following techniques can be used for the detection in a liquid chromatograph? a. Ultraviolet absorbance or refractive index measurement.
More information25 Instruments for Optical Spectrometry
25 Instruments for Optical Spectrometry 25A INSTRUMENT COMPONENTS (1) source of radiant energy (2) wavelength selector (3) sample container (4) detector (5) signal processor and readout (a) (b) (c) Fig.
More informationBasics of UV-Visible Spectroscopy *
OpenStax-CNX module: m34525 1 Basics of UV-Visible Spectroscopy * Brittany L. Oliva-Chatelain Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons Attribution
More informationSpectrophotometric Determination of Iron
Spectrophotometric Determination of Iron INTRODUCTION Many investigations of chemical species involve the interaction between light and matter. One class of these investigations, called absorbance spectrophotometry,
More informationGENERAL PHARMACOPOEIA MONOGRAPH
MINISTRY OF HEALTH OF THE RUSSIAN FEDERATION GENERAL PHARMACOPOEIA MONOGRAPH Spectrophotometry in the ultraviolet GPM.1.2.1.1.0003.15 and visible spectral regions Replaces the SPRF X GPM, SPRF XI GPM,
More information2 SPECTROSCOPIC ANALYSIS
2 SPECTROSCOPIC ANALYSIS 2.1 Introduction Chemical analysis falls into two basic categories: qualitative what is present quantitative how much is present Spectroscopy is capable of both types of analysis,
More informationDesign and Development of a Smartphone Based Visible Spectrophotometer for Analytical Applications
Design and Development of a Smartphone Based Visible Spectrophotometer for Analytical Applications Bedanta Kr. Deka, D. Thakuria, H. Bora and S. Banerjee # Department of Physicis, B. Borooah College, Ulubari,
More informationR O Y G B V. Spin States. Outer Shell Electrons. Molecular Rotations. Inner Shell Electrons. Molecular Vibrations. Nuclear Transitions
Spin States Molecular Rotations Molecular Vibrations Outer Shell Electrons Inner Shell Electrons Nuclear Transitions NMR EPR Microwave Absorption Spectroscopy Infrared Absorption Spectroscopy UV-vis Absorption,
More informationAnalytical Technologies in Biotechnology Prof. Dr. Ashwani K Sharma Department of Biotechnology Indian Institute of Technology, Roorkee
Analytical Technologies in Biotechnology Prof. Dr. Ashwani K Sharma Department of Biotechnology Indian Institute of Technology, Roorkee Module - 6 Spectroscopic Techniques Lecture - 2 UV-Visible Spectroscopy
More information3 - Atomic Absorption Spectroscopy
3 - Atomic Absorption Spectroscopy Introduction Atomic-absorption (AA) spectroscopy uses the absorption of light to measure the concentration of gas-phase atoms. Since samples are usually liquids or solids,
More informationChapter 17: Fundamentals of Spectrophotometry
Chapter 17: Fundamentals of Spectrophotometry Spectroscopy: the science that deals with interactions of matter with electromagnetic radiation or other forms energy acoustic waves, beams of particles such
More informationFourier Transform IR Spectroscopy
Fourier Transform IR Spectroscopy Absorption peaks in an infrared absorption spectrum arise from molecular vibrations Absorbed energy causes molecular motions which create a net change in the dipole moment.
More informationProbing Bonding Using Infrared Spectroscopy Chem
Probing Bonding Using Infrared Spectroscopy Chem 112-2011 INTRODUCTION First, watch the short video on how to record an infrared spectrum using an infrared spectrometer, linked at: http://employees.oneonta.edu/viningwj/chem112/labs/ir_video/ir_video_controller.swf
More informationIR Spectrography - Absorption. Raman Spectrography - Scattering. n 0 n M - Raman n 0 - Rayleigh
RAMAN SPECTROSCOPY Scattering Mid-IR and NIR require absorption of radiation from a ground level to an excited state, requires matching of radiation from source with difference in energy states. Raman
More informationSpectroscopy Problem Set February 22, 2018
Spectroscopy Problem Set February, 018 4 3 5 1 6 7 8 1. In the diagram above which of the following represent vibrational relaxations? 1. Which of the following represent an absorbance? 3. Which of following
More informationClinical Chemistry (CHE221) Professor Hicks Week 1. Statistics Made Slightly Less Boring and Introduction to Spectrophotometry. Accuracy vs Precision
Clinical Chemistry (CHE221) Professor Hicks Week 1 Statistics Made Slightly Less Boring and Introduction to Spectrophotometry 3 Accuracy vs Precision Precision is the consistency of a measurement made
More informationBeer's Law and Data Analysis *
OpenStax-CNX module: m15131 1 Beer's Law and Data Analysis * Mary McHale This work is produced by OpenStax-CNX and licensed under the Creative Commons Attribution License 2.0 1 Beer's Law and Data Analysis
More informationCork Institute of Technology. Summer 2005 Instrumental Analysis (Time: 3 Hours) Section A
Cork Institute of Technology Higher Certificate in Science in Applied Biology Award (National Certificate in Science in Applied Biology Award) Answer FIVE questions; answer Section A, TWO questions from
More informationInstrumental Chemical Analysis
L6 page 1 Instrumental Chemical Analysis Ultraviolet and visible spectroscopy Dr. Ahmad Najjar Philadelphia University Faculty of Pharmacy Department of Pharmaceutical Sciences 2 nd semester, 2016/2017
More informationInstrumental Chemical Analysis
L6 page 1 Instrumental Chemical Analysis Ultraviolet and visible spectroscopy Dr. Ahmad Najjar Philadelphia University Faculty of Pharmacy Department of Pharmaceutical Sciences 2 nd semester, 2016/2017
More informationChemistry 524--Final Exam--Keiderling Dec. 12, pm SES
Chemistry 524--Final Exam--Keiderling Dec. 12, 2002 --4-8 pm -- 238 SES Please answer all questions in the answer book provided. Calculators, rulers, pens and pencils are permitted plus one 8.5 x 11 sheet
More informationFTIR Spectrometer. Basic Theory of Infrared Spectrometer. FTIR Spectrometer. FTIR Accessories
FTIR Spectrometer Basic Theory of Infrared Spectrometer FTIR Spectrometer FTIR Accessories What is Infrared? Infrared radiation lies between the visible and microwave portions of the electromagnetic spectrum.
More informationEducational experiment package Volume 1. Molecular spectroscopy
Educational experiment package Volume 1 Molecular spectroscopy Overview Thermo Fisher Scientific is proud to offer a variety of educational experiments for use with Fourier transform infrared (FTIR) spectrometers.
More informationInfra Red Spectroscopy
CH 2252 Instrumental Methods of Analysis Unit I Infra Red Spectroscopy M. Subramanian Assistant Professor Department of Chemical Engineering Sri Sivasubramaniya Nadar College of Engineering Kalavakkam
More informationOptical Properties of Thin Semiconductor Films
Optical Properties of Thin Semiconductor Films Grolik Benno,KoppJoachim October, 31st 2003 1 Introduction Optical experiments provide a good way of examining the properties of semiconductors. Particulary
More informationSkoog Chapter 7 Components of Optical Instruments
Skoog Chapter 7 Components of Optical Instruments General Design of Optical Instruments Sources of Radiation Wavelength Selectors (Filters, Monochromators, Interferometers) Sample Containers Radiation
More informationChem 321 Lecture 18 - Spectrophotometry 10/31/13
Student Learning Objectives Chem 321 Lecture 18 - Spectrophotometry 10/31/13 In the lab you will use spectrophotometric techniques to determine the amount of iron, calcium and magnesium in unknowns. Although
More informationVALLIAMMAI ENGINEERING COLLEGE SRM Nagar, Kattankulathur
VALLIAMMAI ENGINEERING COLLEGE SRM Nagar, Kattankulathur 603 203 DEPARTMENT OF ELECTRONICS AND INSTRUMENTATION ENGINEERING QUESTION BANK V SEMESTER EI6501 Analytical Instruments Regulation 2013 Academic
More informationChem 321 Name Answer Key D. Miller
1. For a reversed-phase chromatography experiment, it is noted that the retention time of an analyte decreases as the percent of acetonitrile (CH 3 CN) increases in a CH 3 CN/H 2 O mobile phase. Explain
More informationInfrared Spectroscopy: Identification of Unknown Substances
Infrared Spectroscopy: Identification of Unknown Substances Suppose a white powder is one of the four following molecules. How can they be differentiated? H N N H H H H Na H H H H H A technique that is
More informationUNIT 2 UV-VISIBLE SPECTROMETRY
Molecular Spectroscopic Methods-I UNIT 2 UV-VISIBLE SPECTROMETRY Structure 2.1 Introduction Objectives 2.2 Origin and Characteristics of UV-VIS Spectrum Origin of UV-VIS spectrum Characteristics of UV-VIS
More informationFLAME PHOTOMETRY AIM INTRODUCTION
FLAME PHOTOMETRY AIM INTRODUCTION Atomic spectroscopy is based on the absorption, emission or fluorescence process of light by atoms or elementary ions. Information for atomic scale is obtained in two
More informationChapter 18. Fundamentals of Spectrophotometry. Properties of Light
Chapter 18 Fundamentals of Spectrophotometry Properties of Light Electromagnetic Radiation energy radiated in the form of a WAVE caused by an electric field interacting with a magnetic field result of
More informationSpectroscopy: Introduction. Required reading Chapter 18 (pages ) Chapter 20 (pages )
Spectroscopy: Introduction Required reading Chapter 18 (pages 378-397) Chapter 20 (pages 424-449) Spectrophotometry is any procedure that uses light to measure chemical concentrations Properties of Light
More informationSPECTROPHOTOMETRY AND SPECTROMETRY - CONCEPT AND APPLICATIONS
SPECTROPHOTOMETRY AND SPECTROMETRY - CONCEPT AND APPLICATIONS Renjini A 1, Dani Dileep 2 1 Assistant Professor, Department of ECE, Rajadhani Institute of Engineering and Technology, Kerala, India 2 PG
More informationAbsorption photometry
The light Absorption photometry Szilvia Barkó University of Pécs, Faculty of Medicines, Dept. Biophysics February 2011 Transversal wave E Electromagnetic wave electric gradient vector wavelength The dual
More informationInfrared Spectroscopy
Infrared Spectroscopy IR Spectroscopy Used to identify organic compounds IR spectroscopy provides a 100% identification if the spectrum is matched. If not, IR at least provides information about the types
More informationMOLEBIO LAB #4: Using a Spectrophotometer
Introduction: Spectrophotometry MOLEBIO LAB #4: Using a Spectrophotometer Many kinds of molecules interact with or absorb specific types of radiant energy in a predictable fashion. For example, when while
More informationPART SPECTROPHOTOMETRIC STUDIES
PART SPECTROPHOTOMETRIC STUDIES CHAPTER General Principles of Spectrophotometry 123 In absorption spectroscopy, absorption measurements based upon ultraviolet light and visible radiation find application
More informationtwo slits and 5 slits
Electronic Spectroscopy 2015January19 1 1. UV-vis spectrometer 1.1. Grating spectrometer 1.2. Single slit: 1.2.1. I diffracted intensity at relative to un-diffracted beam 1.2.2. I - intensity of light
More information4.3A: Electronic transitions
Ashley Robison My Preferences Site Tools Popular pages MindTouch User Guide FAQ Sign Out If you like us, please share us on social media. The latest UCD Hyperlibrary newsletter is now complete, check it
More informationGlossary. Analyte - the molecule of interest when performing a quantitative analysis.
Glossary This glossary contains definitions of many important FTIR terms. Many of the terms listed here appeared in italics in the body of the book. Words that appear in italics in the glossary are defined
More informationModern Instrumental Methods of Analysis Prof. J. R. Mudakavi Department of Chemical Engineering Indian Institute of Science, Bangalore
Modern Instrumental Methods of Analysis Prof J R Mudakavi Department of Chemical Engineering Indian Institute of Science, Bangalore Module No # 02 Lecture No # 07 Ultraviolet and Visible Spectrophotometry
More informationExperiment 1 (Part A): Plotting the Absorption Spectrum of Iron (II) Complex with 1,10- Phenanthroline
Experiment (Part A): Plotting the Absorption Spectrum of Iron (II) Complex with,0- Phenanthroline Background The first step of an analytical spectrophotometric procedure for quantitative determination
More informationChemistry 524--Final Exam--Keiderling May 4, :30 -?? pm SES
Chemistry 524--Final Exam--Keiderling May 4, 2011 3:30 -?? pm -- 4286 SES Please answer all questions in the answer book provided. Calculators, rulers, pens and pencils are permitted. No open books or
More informationCourse Details. Analytical Techniques Based on Optical Spectroscopy. Course Details. Textbook. SCCH 211: Analytical Chemistry I
SCCH 211: Analytical Chemistry I Analytical Techniques Based on Optical Spectroscopy Course Details September 22 October 10 September 22 November 7 November 17 December 1 Topic Period Introduction to Spectrometric
More informationElectronic Excitation by UV/Vis Spectroscopy :
SPECTROSCOPY Light interacting with matter as an analytical tool III Pharm.D Department of Pharmaceutical Analysis SRM College Of Pharmacy,Katankulathur Electronic Excitation by UV/Vis Spectroscopy : X-ray:
More informationReflection = EM strikes a boundary between two media differing in η and bounces back
Reflection = EM strikes a boundary between two media differing in η and bounces back Incident ray θ 1 θ 2 Reflected ray Medium 1 (air) η = 1.00 Medium 2 (glass) η = 1.50 Specular reflection = situation
More informationMOLECULAR AND ATOMIC SPECTROSCOPY
MOLECULAR AND ATOMIC SPECTROSCOPY 1. General Background on Molecular Spectroscopy 3 1.1. Introduction 3 1.2. Beer s Law 5 1.3. Instrumental Setup of a Spectrophotometer 12 1.3.1. Radiation Sources 13 1.3.2.
More informationFourier Transform Infrared. Spectrometry
Fourier Transform Infrared. Spectrometry Second Editio n PETER R. GRIFFITH S JAMES A. de HASETH PREFACE x v CHAPTER 1 INTRODUCTION TO VIBRATIONAL SPECTROSCOPY 1 1.1. Introduction 1 1.2. Molecular Vibrations
More information( ) x10 8 m. The energy in a mole of 400 nm photons is calculated by: ' & sec( ) ( & % ) 6.022x10 23 photons' E = h! = hc & 6.
Introduction to Spectroscopy Spectroscopic techniques are widely used to detect molecules, to measure the concentration of a species in solution, and to determine molecular structure. For proteins, most
More informationBeer's- Lambert Law and Standard Curves. BCH 312 [Practical]
Beer's- Lambert Law and Standard Curves BCH 312 [Practical] Spectrophotometer: Spectrophotometer can be used to measure the amount of light absorbed or transmitted by a solution. It consist of two parts:
More informationEvaluating Labsphere s new UV-2000
Ciba Grenzach GmbH Optical Spectroscopy Sabrina Siegle Dr. Wolfgang Schlenker Tel. +49 76 24 / 12-28 13 E-mail wolfgang.schlenker@ciba.com May 26, 9 Evaluating Labsphere s new UV- Abstract Labsphere s
More informationSpectrochemical methods
Spectrochemical methods G. Galbács The interactions of radiations and matter are the subject of spectroscopy py or spectrochemical methods (also called spectrometry). Spectrochemical methods usually measure
More informationAIM To verify Beer - Lambert s law and to determine the dissociation constant (Ka) of methyl red, Spectrophotometrically.
C 141(Expt. No. ) NAME : ROLL No. : SIGNATURE : BATCH : DATE : VERIFICATION OF BEER - LAMBERT S LAW & DETERMINATION OF DISSOCIATION CONSTANT (Ka) OF METHYLRED, SPECTROPHOTOMETRICALLY AIM To verify Beer
More informationChemistry Instrumental Analysis Lecture 18. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 18 Instrumentation Radiation sources Hollow cathode lamp Most common source Consist of W anode and a cathode sealed in a glass tube filled with Ne or Ar. Hollow
More informationSecondary Ion Mass Spectrometry (SIMS)
CHEM53200: Lecture 10 Secondary Ion Mass Spectrometry (SIMS) Major reference: Surface Analysis Edited by J. C. Vickerman (1997). 1 Primary particles may be: Secondary particles can be e s, neutral species
More informationLecture 3: Light absorbance
Lecture 3: Light absorbance Perturbation Response 1 Light in Chemistry Light Response 0-3 Absorbance spectrum of benzene 2 Absorption Visible Light in Chemistry S 2 S 1 Fluorescence http://www.microscopyu.com
More informationEXPERIMENT #3 A Beer's Law Study
OBJECTVES: EXPERMENT #3 A Beer's Law Study To operate a Spectronic 20 To convert from percent transmission to absorbance units To plot absorbance versus wavelength and find max To plot absorbance versus
More informationUV / Visible Spectroscopy. Click icon to add picture
UV / Visible Spectroscopy Click icon to add picture Spectroscopy It is the branch of science that deals with the study of interaction of matter with light. OR It is the branch of science that deals with
More information