Enzymatic Assay of NITRATE REDUCTASE (EC )
|
|
- Stanley Pitts
- 5 years ago
- Views:
Transcription
1 Enzymatic Assay of NITRATE REDUCTASE PRINCIPLE: Nitrate + ß-NADH Nitrate Reductase > Nitrite + ß-NAD + H 2 O Nitrite + Sulfanilamide + NED > Nitrite Color Complex Abbreviations used: ß-NADH = ß-Nicotinamide Adenine Dinucleotide, Reduced Form ß-NAD = ß-Nicotinamide Adenine Dinucleotide, Oxidized Form NED = N-(1-Naphthyl)ethylenediamine Dihydrochloride CONDITIONS: T = 3 C, ph = 7.3, A 54nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Determination REAGENTS: A. 25 mm Potassium Phosphate Buffer with 1 mm Potassium Nitrate and.5 mm Ethylenediaminetetraacetic Acid, ph 7.3 at 3 C (Prepare 1 ml in deionized water using Potassium Phosphate, Monobasic, Anhydrous, Potassium Nitrate, and Ethylenediaminetetraacetic Acid, Disodium Salt, Dihydrate.) B. 2. mm ß-Nicotinamide Adenine Dinucleotide, Reduced Form Solution (ß-NADH) (Dissolve the contents of one 1 mg vial of ß-Nicotinamide Adenine Dinucleotide, Reduced Form, Disodium Salt, in the appropriate volume of deionized water. PREPARE FRESH.) C. 3 M Hydrochloric Acid Solution (HCl) (Prepare 1 ml in deionized water using Hydrochloric Acid.) D. 58 mm Sulfanilamide Solution (Sulf) (Prepare 1 ml in Reagent C using Sulfanilamide.) Page 1 of 5
2 REAGENTS: (continued) Enzymatic Assay of NITRATE REDUCTASE E..77 mm N-(1-Naphthyl)ethylenediamine Dihydrochloride Solution (NED) (Prepare 1 ml in deionized water using N-(1- Naphthyl)ethylenediamine Dihydrochloride.) F mm Nitrate Standard Solution (Std Soln) (Prepare 5 ml in Reagent A using Sodium Nitrite.) G. Nitrate Reductase Enzyme Solution (Immediately before use, prepare a soluiton containing.2-.6 unit/ml of Nitrate Reductase in cold Reagent A.) PROCEDURE: Pipette (in milliliters) the following reagents into suitable containers 1 : Test Blank Std1 Std2 Std3 Std4 Std5 Reagent A (Buffer) Reagent B (ß-NADH) Reagent F (Std Soln) Mix by swirling and equilibrate to 3 C. Then add: Reagent G (Enz Soln) Immediately mix by swirling and incubate at 3 C for exactly 2 minutes. Stop the reaction by adding: Reagent D (Sulf) Page 2 of 5
3 Mix thoroughly by swirling, then add: Reagent E (NED) Mix by swirling and incubate for 1 minutes at 25 C. Transfer the solutions to suitable cuvettes and record the A 54nm for the Test, Blank and Standards using a suitable spectrophotometer. CALCULATIONS: Standard Curve: ra 54nm Standard = A 54nm Std - A 54nm Std Blank Page 3 of 5
4 CALCULATIONS: (continued) Enzymatic Assay of NITRATE REDUCTASE Prepare a standard curve by plotting the?a 54nm of the Standard versus µmoles of nitrite. Sample Determination: ra 54nm Sample = A 54nm Test - A 54nm Test Blank Determine the µmoles of nitrite formed using the standard curve. (µmole Nitrite formed)(df) Units/ml enzyme = (2)(.1) df = Dilution factor 2 = Time of assay (in minutes).1 = Volume of enzyme (in milliliter) used units/ml enzyme Units/mg solid = mg solid/ml enzyme units/ml enzyme Units/mg protein = mg protein/ml enzyme UNIT DEFINITION: One unit will reduce 1. µmole of nitrate to nitrite per minute in a ß-NADH system at ph 7.3 at 3 C. FINAL ASSAY CONCENTRATION: In a 2. ml reaction mix, the final concentrations are 24 mm potassium phosphate,.5 mm ethylenediaminetetraacetic acid, 9.5 mm potassium nitrate,.1 mm ß-nicotinamide adenine dinucleotide, reduced form, and unit nitrate reductase. REFERENCE: Redinbaugh, M.G. and Campbell, W.H. (1985) Journal of Biological Chemistry 26, Page 4 of 5
5 Enzymatic Assay of NITRATE REDUCTASE REFERENCES: NOTES: Smarrelli, Jr., J. and Campbell, W.H. (1983) Biochimica et Biophysica Acta 742, Perform the color development of the Test and Standards at the same time in order to prevent deviations in color development from the standard curve. 2. This assay is based on the cited references. 3. Where our Product or Stock numbers are specified, equivalent reagents may be substituted. This procedure is for informational purposes. For a current copy of our quality control procedure contact our Technical Service Department. Page 5 of 5
F. 2.6 mm a-ketoglutaric Acid Solution (KG) (Prepare 1 ml in Reagent A using a-ketoglutaric Acid, Disodium Salt.)
Enzymatic Assay of L-GLUTAMATE OXIDASE PRINCIPLE: L-Glutamate + O 2 + H 2 O L-Glutamate Oxidase > a-ketoglutaric Acid + NH 3 + H 2 O 2 2 H 2 O 2 + H 2 O Catalase > 2 H 2 O + O 2 CONDITIONS: T = 30 C, ph
More informationEnzymatic Assay of GALACTOSYLTRANSFERASE (EC )
Enzymatic Assay of GALACTOSYLTRANSFERASE PRINCIPLE: UDP-Galactose + D-Glucose Galactosyltransferase > UDP + Lactose UDP + PEP PK > Pyruvate + UTP Pyruvate + ß-NADH LDH > Lactate + ß-NAD Abbreviations used:
More informationRUBISCO > 2 moles of 3-phosphoglycerate Mg +2
PRINCIPLE: RuDP + CO 2 RUBISCO > 2 moles of 3-phosphoglycerate Mg +2 3-Phosphoglycerate + ATP PGK > Glycerate 1,3-Diphosphate + ADP Glycerate 1,3-Diphosphate + ß-NADH GAPDH > Glyceraldehyde 3-Phosphate
More informationEnzymatic Assay of GUANYLATE KINASE (EC )
PRINCIPLE: GMP + ATP Guanylate Kinase > GDP + ADP ADP + PEP Pyruvate Kinase > ATP + Pyruvate GDP + PEP Pyruvate Kinase > GTP + Pyruvate 2 Pyruvate + 2 ß-NADH Lactic Dehydrogenase > 2 Lactate + 2 ß-NAD
More informationEnzymatic Assay of GLYCOGEN SYNTHASE (EC )
PRINCIPLE: UDPG + (Glycogen) n Glycogen Synthase > UDP + (Glycogen) n+1 UDP + PEP PK > UTP + Pyruvate Pyruvate + ß-NADH LDH > Lactate + ß-NAD Abbreviations: UDPG = Uridine 5'-Diphosphoglucose UDP = Uridine
More informationEnzymatic Assay of LYSINE OXIDASE (EC )
PRINCIPLE: L-Lysine + O 2 + H 2 O Lysine Oxidase 6-Amino-2-Oxohexanoate + NH 3 + H 2 O 2 H 2 0 2 + 6-Amino-2-Oxohexanoate Catalase >? 1 -Piperidine-2-Carboxylate? 1 -Piperidine-2-Carboxylate MBTH > UV
More informationEnzymatic Assay of ACTOMYOSIN ATPase Assay
Enzymatic Assay of ACTOMYOSIN PRINCIPLE: ATP + H 2 O ATPase > ADP + P i Abbreviations used: ATP = Adenosine 5'-Triphosphate ATPase = Adenosine 5'-Triphosphatase ADP = Adenosine 5'-Diphosphate P i = Inorganic
More informationEnzymatic Assay of CALCINEURIN
PRINCIPLE: The assay for Calcineurin is based on its ability to bind to Calmodulin. Calmodulin which is bound to Calcineurin is no longer available to activate Phosphodiesterase 3':5'-Cyclic Nucleotide.
More informationEnzymatic Assay of HYPOXANTHINE-GUANINE PHOSPHORIBOSYL TRANSFERASE (EC )
PRINCIPLE: PRPP + Guanine HGPRT > GMP + Pyrophosphate Mg ++ GMP + ATP GK > GDP + ADP ADP + PEP PK > ATP + Pyruvate GDP + PEP PK > GTP + Pyruvate 2 Pyruvate + 2 ß-NADH LDH > 2 Lactate + 2 ß-NAD Abbreviations
More informationEnzymatic Assay of NITRIC OXIDE SYNTHETASE (EC ) ß-NADP = ß-Nicotinamide Adenine Dinucleotide Phosphate,
PRINCIPLE: L-Arginine + ß-NADPH + O2 Nitric Oxide Synthetase > Citrulline + NO + ß-NADP Ca 2+, Tetrahydrobiopterin, Calmodulin Abbreviations used: ß-NADPH = ß-Nicotinamide Adenine Dinucleotide Phosphate,
More informationEnzymatic Assay of CARBOXYPEPTIDASE P (EC )
PRINCIPLE: N-CBZ-Glu-Tyrosine + H2O Carboxypeptidase P > N-CBZ-L-Glutamic Acid + L-Tyrosine Abbreviation used: N-CBZ = N-Carbobenzoxy CONDITIONS: T = 30 C, ph = 3.7, A 570nm, Light path = 1 cm METHOD:
More informationEnzymatic Assay of LAMINARINASE 1 (EC )
PRINCIPLE: Laminarin + H 2 O Laminarinase > Reducing Sugar (measured as glucose) CONDITIONS: T = 37 C, ph = 5.0, A 540nm, Light path = 1 cm METHOD: Colorimetric REAGENTS: A. 100 mm Sodium Acetate Buffer,
More informationEnzymatic Assay of PHOSPHOLIPASE D (EC )
PRINCIPLE: L-α-Phosphatidylcholine + H 2 O Phospholipase D > Choline + Phosphatidic Acid Choline + O 2 + H 2 O Choline Oxidase > Betaine Aldehyde + H 2 O 2 2H 2 O 2 + 4-AAP + Phenol Peroxidase > 4H 2 O
More informationEnzymatic Assay of POLYGALACTURONASE (EC )
PRINCIPLE: Polygalacturonic Acid + H 2 O PG > Reducing Sugars Abbreviations: PG = Polygalacturonase CONDITIONS: T = 30 C, ph 5.0, A 540nm, Light path = 1 cm METHOD: Colorimetric REAGENTS: A. 50 mm Sodium
More informationEnzymatic Assay of TRYPTOPHANASE (EC )
Enzymatic Assay of TRYPTOPHANASE PRINCIPLE: PRP + L-Tryptophan + H 2 O Tryptophanase > Indole + Pyruvate + NH 3 Abbreviation: PRP = Pyridoxal 5-Phosphate CONDITIONS: T = 37 C, ph 8.3, A 540nm, Light path
More informationA. 50 mm Sodium Lauryl Sulfate Solution (SDS) (Prepare 10 ml in deionized water using Lauryl Sulfate, Sodium Salt, Sigma Prod. No. L-5750.
SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHOLIPASE D 1 (EC 3.1.4.4) PRINCIPLE: L-α-Phosphatidylcholine + 2H 2 O Phospholipase D > Choline + Phosphatidic Acid 2 Choline + O 2 Choline
More informationEnzymatic Assay of PHOSPHOLIPASE C, PHOSPHATIDYLINOSITOL-SPECIFIC (EC )
PRINCIPLE: Step 1: Enzymatic Assay of PHOSPHOLIPASE C, PHOSPHATIDYLINOSITOL-SPECIFIC Acetylcholinesterase (membrane stroma bound) PLP C > Acetylcholinesterase (unbound) Step 2: ATI + DTNB Acetylcholinesterase
More informationEnzymatic Assay of PAPAIN 1 (EC )
PRINCIPLE: BAEE + H 2 O Papain > Na-Benzoyl-L-Arginine + Ethanol Abbreviation used: BAEE = Na-Benzoyl-L-Arginine Ethyl Ester CONDITIONS: T = 25 C, ph = 6.2 METHOD: Titrimetric Rate Determination REAGENTS:
More informationEnzymatic Assay of CHITINASE (EC )
PRINCIPLE: Chitin + H 2 O Chitinase > Chitobiose Chitobiose + H 2 O ß-N-Acetylglucosaminidase > N-Acetyl-D-Glucosamine CONDITIONS: T = 25 C, ph = 6.0, A 540nm, Light path = 1 cm METHOD: Colorimetric REAGENTS:
More informationEnzymatic Assay of PEPTIDYL ARGININE DEIMINASE (EC )
PRINCIPLE: BAEE + H 2 O PAD > Nα-Benzoyl-L-Citrulline + Ethanol Abbreviation used: PAD = Peptidyl Arginine Deiminase CONDITIONS: T = 55 C, ph 7.2, A 490nm, Light path = 1 cm METHOD: Colorimetric REAGENTS:
More informationEnzymatic Assay of ANCROD (EC )
Enzymatic Assay of ANCROD PRINCIPLE: Fibrinogen Ancrod > Fibrin CONDITIONS: T = 37 C, ph 7.35 METHOD: Fibrometer REAGENTS: A. 143 mm 5,5-Diethylbarbiturate and 143 mm Sodium Acetate Solution (Soln A) (Prepare
More informationEnzymatic Assay of FK-BINDING PROTEIN Peptidyl-Prolyl Isomerase Activity
Enzymatic Assay of FK-BINDING PROTEIN PRINCIPLE: Suc-Ala-Leu-cis-Pro-Phe-pNA FKBP > Suc-Ala-Leu-trans-Pro-PhepNA Suc-Ala-Leu-trans-Pro-Phe-pNA + H2O Chymotrypsin > Suc-Ala-Leu-trans-Pro-Phe + p-nitroaniline
More informationEnzymatic Assay of PROTEASE INHIBITOR, of Calcium Activated Neutral Protease
PRINCIPLE: N,N-Dimethylated Casein + H 2 O Protease, Calcium Activated Neutral > Amino Acids This reaction is inhibited by the protease inhibitor. CONDITIONS: T = 30 C, ph = 7.5, A 293nm, Light path =
More informationEnzymatic Assay of CASEIN KINASE II
PRINCIPLE: Casein +?- 32 P-ATP Casein Kinase II > [ 32 P]-Phosphorylated Casein + ADP CONDITIONS: T = 37 C, ph 7.5 METHOD: Radiolabelled Stop Reaction REAGENTS: A. 100 mm HEPES Buffer, ph 7.5 at 37 C (Enzyme
More information1.2 Systematic Name: D-Amino acid: oxygen reductase (deaminating)
Document Title D-Amino Acid Oxidase Page 1 of 5 Originating Department QA Approval Departments QA, QC Approval Date 30 th August 2016 Effective Date 7 th October 2016 1.0 PRODUCT DETAILS 1.1 Enzyme Name:
More informationColorimetric Assay for Nitric Oxide Product No For Research Use Only
Colorimetric Assay for Nitric Oxide Product No. 430410 For Research Use Only Store Nitrate Reductase Enzyme at 20 C NADH should be stored in the dark at room temperature Store all other kit components
More informationEnzymatic Assay of PROTEIN KINASE C
PRINCIPLE: Histone +? 32 P-ATP Protein Kinase > [ 32 P]-Phosphorylated Histone + ADP Abbreviations used:? 32 P-ATP = Adenosine 5'-Triphosphate? 32 P-labelled ADP = Adenosine 5'-Diphosphate CONDITIONS:
More informationNitric Oxide Synthase Ultrasensitive Colorimetric Assay
Package Insert Nitric Oxide Synthase Ultrasensitive Colorimetric Assay 96 Wells For Research Use Only v. 2.0 09.20.17 Eagle Biosciences, Inc. 20A NW Blvd., Suite 112, Nashua, NH 03063 Phone: 866-419-2019
More informationThe appearance of phenolphthalein is measured spectrophotometrically at 540nm (2).
Document Title β-glucuronidase Page 1 of 5 Originating Department QC Approval Departments QA, QC Approval Date 23 rd May 2017 Effective Date 8 th June 2017 1.0 PRODUCT DETAILS 1.1 Enzyme Name: β-glucuronidase
More informationNECi Nitrate Kits FAQs
Why use enzymes? Enzymes are catalysts that drive complex biological reactions. They happen to be excellent reagents for analytical chemistry because they are reliable, accurate, sensitive, selective,
More informationDETERMINATION OF NITRATE IN 2M KCl SOIL EXTRACTS BY FLOW INJECTION ANALYSIS NITRATE REDUCTASE
QuikChem Method 12-107-04-5-A DETERMINATION OF NITRATE IN 2M KCl SOIL EXTRACTS BY FLOW INJECTION ANALYSIS NITRATE REDUCTASE (LOW FLOW METHOD) Written by Scott Tucker Applications Group Revision Date: 28
More informationDETERMINATION OF NITRATE IN BRACKISH WATERS BY FLOW INJECTION ANALYSIS NITRATE REDUCTASE
QuikChem Method 31-107-04-5-A DETERMINATION OF NITRATE IN BRACKISH WATERS BY FLOW INJECTION ANALYSIS NITRATE REDUCTASE (LOW FLOW METHOD) Written by Scott Tucker Applications Group Revision Date: 11 February
More informationCERTIFICATE OF ANALYSIS Nitric Oxide Assay Kit
Ordering Code: EMSNO CERTIFICATE OF ANALYSIS Nitric Oxide Assay Kit Lot Number: MG157087 Product: A complete kit for the quantitative determination of nitrite and nitrate in biological fluids (2 Plate,
More informationColorimetric GAPDH Assay Cat. No. 8148, 100 tests
Colorimetric GAPDH Assay Cat. No. 8148, 1 tests Introduction Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for
More informationNAD + /NADH Assay [Colorimetric]
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name NAD + /NADH Assay [Colorimetric] (Cat. #786 1539, 786 1540) think proteins! think G-Biosciences
More informationCertificate of Analysis
Certificate of Analysis 10 Old Barn Road Lake Placid, NY 12946 Technical Support: T: 800 548-7853 F: 518 523-4513 email: techserv@upstate.com Sales Department: T: 800 233-3991 F: 781 890-7738 Licensing
More informationApplication Note: A TD-700 Laboratory Fluorometer Method for Alkaline Phosphatase Fluorescence
1. INTRODUCTION Because of their critical functions in eukaryotic cells, methods for measuring protein phosphatases were established at least as early as 1953 1. In 1965 Fernley and Walker 2 decribed the
More informationVERSION FOR SIEMENS DIMENSION VISTA 1500 AUTOMATED ANALYZER
VERSION FOR SIEMENS DIMENSION VISTA 1500 AUTOMATED ANALYZER NON SUPPLIED ADDITIONAL EQUIPMENT: Preparation of the reagents requires the use of micropipettes or systems suitable for dispensing the specified
More informationVERSION FOR WERFEN ILAB 600 AND 650 AUTOMATED ANALYZER
VERSION FOR WERFEN ILAB 600 AND 650 AUTOMATED ANALYZER NON SUPPLIED ADDITIONAL EQUIPMENT: Preparation of the reagents requires the use of micropipettes or systems suitable for dispensing the specified
More informationNAD/NADH Microplate Assay Kit User Manual
NAD/NADH Microplate Assay Kit User Manual Catalog # CAK1008 Detection and Quantification of NAD/NADH Content in Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological
More informationExperiment 4 - BIOC 221 W2019. The Subject for the should be: BIOCHEM 221 EXP4
EXCEL SHEET INSTRUCTIONS 1. a) On the web page there is a file called YOURNAME_Bioc221Exp4W2019.xlsx. Fill in the information colored RED with your own information. (Please do not readjust where the cells
More informationCitric acid Enzymatic method
Method OIV-MA-AS313-09 Type II method Enzymatic method 1. Principle is converted into oxaloacetate and acetate in a reaction catalyzed by citratelyase (CL): Citrate CL oxaloacetate + acetate In the presence
More informationLab 3: Protein Determination and Enzyme Assay of Crab and Onion Samples
Lab 3: Protein Determination and Enzyme Assay of Crab and Onion Samples Khandi Coffman, Satoshi Sagami, Tramanh Do, Michaela Smith and Melissa Kindhart Lab 3: Protein Determination and Enzyme Assay of
More informationNitric Oxide Synthase Colorimetric Non-enzymatic Assay
Package Insert Nitric Oxide Synthase Colorimetric Non-enzymatic Assay 96 Wells For Research Use Only v. 1.0 Eagle Biosciences, Inc. 20A NW Blvd., Suite 112, Nashua, NH 03063 Phone: 866-419-2019 Fax: 617-419-1110
More informationAlcohol dehydrogenase Assay Kit
Alcohol dehydrogenase Assay Kit Catalog Number KA3785 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General
More informationEncapsulated enzymes with integrated fluorescence-control of enzymatic activity. Supporting Information
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is The Royal Society of Chemistry 2015 Pascal K. Harimech, 1# Raimo Hartmann, 1# Joanna Rejman, 1 Pablo del Pino,
More informationStandard Methods for the Examination of Water and Wastewater
4500-NO 2 NITROGEN (NITRITE)*#(1) 4500-NO 2 A. Introduction 1. Occurrence and Significance For a discussion of the chemical characteristics, sources, and effects of nitrite nitrogen, see Section 4500-N.
More informationNitric Oxide Synthase Assay Kit
Nitric Oxide Synthase Assay Kit Catalog Number KA1634 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay...
More informationNitrate Reductase (NR) Colorimetric Assay Kit
(FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSIS!) Nitrate Reductase (NR) Colorimetric Assay Kit Catalog No: E-BC-K158 Method: Colorimetric method Specification: 100 Assays This manual must
More informationDetermination of Orthophosphate Ion
Determination of Orthophosphate Ion Introduction Phosphorous, in the form of phosphate, is one of several important elements in the growth of plants. Excessive algae growth in water is stimulated by the
More informationDetermination of Orthophosphate Ion
Determination of Orthophosphate Ion Introduction Phosphorous, in the form of phosphate, is one of several important elements in the growth of plants. Excessive algae growth in water is stimulated by the
More informationL-malic acid measurement in wines using the SpectraMax Plus 384 Microplate Reader
APPLICATION NOTE L-malic acid measurement in wines using the SpectraMax Plus 384 Microplate Reader Introduction Analysis of malic acid, residual sugar, volatile acidity and ammonia is very important for
More informationExperiment 5: Analysis of Nutrients in Natural Waters CH3600 / ESP 5090: Environmental Chemistry, Plymouth State University
Experiment 5: Analysis of Nutrients in Natural Waters CH3600 / ESP 5090: Environmental Chemistry, Plymouth State University Adapted from "Experiment 3: Analysis of Phosphate in Water," Laboratory Experiments
More informationNAD/NADH Assay Kit. Catalog Number KA assays Version: 03. Intended for research use only.
NAD/NADH Assay Kit Catalog Number KA3777 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...
More information13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment 131 Learning Objective This laboratory introduces you to steady-state kinetic analysis, a fundamental
More informationMethod of Enzyme Assay
Method of Enzyme Assay Objective To study the different methods for determining enzyme activity. Enzyme assays: Are laboratory methods for measuring enzymatic activity. All enzyme assays measure either
More informationMethod of Enzyme Assay
Method of Enzyme Assay Objective To study the different methods for determining enzyme activity. Use these method in diagnosis of certain diseases How to follow a reaction? Enzyme assays: Are laboratory
More informationFluoro NADP/NADPH Fluorescent NADP/NADPH Detection Kit
Fluoro NADP/NADPH Fluorescent NADP/NADPH Detection Kit Contact Information Address Telephone Toll Free Fax General Information Sales Technical Questions Website Cell Technology Inc 950 Rengstorff Ave Suite
More informationWHAT YOU NEED. Visible Spectrophotometer. Micropipettes. Cuvettes. Parafilm. Timer µL
WHAT YOU NEED Visible Spectrophotometer Micropipettes 10-100µL 100-1000µL Cuvettes Parafilm Timer WHAT TESTS CAN BE DONE Acetic Acid Amino Acid Nitrogen Ammonia Citric Acid Glucose & Fructose Lactic Acid
More informationNitrate plus Nitrite and Nitrite in Seawater by Segmented Flow Analysis (SFA)
Methodology Nitrate plus Nitrite and Nitrite in Seawater by Segmented Flow Analysis (SFA) (Cartridge Part #A002603) 1.0 Scope and Application 1.1 This method is used to determine the concentration of nitrate
More informationDetermination of Dissolved Inorganic Nitrate plus Nitrite (NO3+NO2) in Fresh/Estuarine/Coastal Waters Using Enzyme Catalyzed Reduction
Determination of Dissolved Inorganic Nitrate plus Nitrite (NO3+NO2) in Fresh/Estuarine/Coastal Waters Using Enzyme Catalyzed Reduction 1. SCOPE and APPLICATION 1.1 Enzyme catalyzed reduction is used to
More informationNWLSS TM Nitric Oxide (Nitrate/Nitrite) Non-Enzymatic Assay
Premier Products for Superior Life Science Research NWLSS TM Nitric Oxide (Nitrate/Nitrite) Non-Enzymatic Assay Product NWK-NNO01 For Research Use Only Non-enzymatic assay system for measurement of nitric
More informationALDH Activity Assay Kit (Colorimetric)
ALDH Activity Assay Kit (Colorimetric) Catalog Number KA3742 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationDTNB-Thiols Assay Kit
DTNB-Thiols Assay Kit Catalog Number KA4410 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...
More informationGlutathione Peroxidase Assay Kit
Glutathione Peroxidase Assay Kit Catalog Number KA1628 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationRat Advanced Glycation End Products(AGEs) ELISA Kit
Rat Advanced Glycation End Products(AGEs) ELISA Kit Catalog No. CSB-E09413r (96 T) This immunoassay kit allows for the in vitro quantitative determination of rat AGEs concentrations in serum, plasma and
More informationProtein assay. Absorbance Fluorescence Emission Colorimetric detection BIO/MDT 325. Absorbance
Protein assay Absorbance Fluorescence Emission Colorimetric detection BIO/MDT 325 Absorbance Using A280 to Determine Protein Concentration Determination of protein concentration by measuring absorbance
More informationAppendix: 1. Sodium bicarbonate 0.84 gm (10 mm/l) 50ml of 2% sodium carbonate in 0.10N sodium hydroxide
Appendix: 1 Chemicals, Reagents and Buffers 1. BUFFERS FOR WBC EXTRACTION WBC lysis buffer (for 1 liter) Ammonium chloride 8.3 gm (150 mm/l) Sodium bicarbonate 0.84 gm (10 mm/l) 1 X reagent EDTA 29 mg
More informationDETERMINATION OF NITRATE AND NITRITE BY ZONE FLUIDICS
DETERMINATION OF NITRATE AND NITRITE BY ZONE FLUIDICS SPECTROPHOTOMETRIC DETECTION WRITTEN BY GRAHAM MARSHALL Revision: - Date: April 19, 2014 met_nox_-.docx Page 1 DETERMINATION OF NITRATE AND NITRITE
More informationMeasurement of L-Malic Acid in Wines Using the SpectraMax Plus 384 Microplate Reader
now part of MDS Analytical Technologies Measurement of L-Malic Acid in Wines Using the SpectraMax Plus 384 Microplate Reader SpectraMax Application Note #18 By Theresa Heredia and Gillian Sutherland from
More informationModified Adams Assay for Phenolics in Wine
Modified Adams Assay for Phenolics in Wine 1. Total Iron-Reactive Phenolics THIS VALUE WILL DETERMINE DILUTIONS FOR TANNIN & POLYMERIC PIGMENT ANALYSES 1.1 Into a reduced volume cuvette, pipette in the
More informationMicrodetermination of phosphorus using the SPECTRAmax PLUS microplate spectrophotometer: choice of microplate, cuvette or test tube assay formats
Application Note 24 Microdetermination of phosphorus using the SPECTRAmax PLUS microplate spectrophotometer: choice of microplate, cuvette or test tube assay formats INTRODUCTION Most spectrophotometric
More informationshown by line A. The net effect of temperature on an enzyme-catalyzed reaction is given by line C.
Experiment 8 EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Temperature affects the stability of an enzyme as well as the binding of substrate and its transformation to product. Line B of Fig. 8-l shows the
More information1) Alkaline Persulfate Digestion for TN, TDN and TDPTotal Dissolved Nitrogen. a) Scope and Application
1) Alkaline Persulfate Digestion for TN, TDN and TDPTotal Dissolved Nitrogen a) Scope and Application i) This method covers the digestion procedure for the determination of total dissolved nitrogen (TDN)
More informationContact Person(s) : Anna Berne APPLICATION
Se-03 AMERICIUM, PLUTONIUM AND URANIUM IN WATER Contact Person(s) : Anna Berne APPLICATION This procedure describes a method for the separation and measurement of americium, plutonium and uranium in water
More informationMalachite Green Phosphate Detection Kit Catalog Number: DY996
Malachite Green Phosphate Detection Kit Catalog Number: DY996 This Malachite Green Phosphate Detection Kit employs a simple, sensitive, reproducible, and non-radioactive method for measuring inorganic
More informationSubstrate Specificity of Alcohol Dehydrogenase
0 Substrate Specificity of Alcohol Dehydrogenase Roshan Roshan Chikarmane and Jonathan White Department of Chemistry University of Oregon Eugene, OR 97403 April 26, 2014 Abstract: The substrate specificity
More informationL-RHAMNOSE
www.megazyme.com L-RHAMNOSE ASSAY PROCEDURE K-RHAMNOSE 08/18 (*50/100 Manual Assays per Kit) or (550 Auto-Analyser Assays per Kit) or (550 Microplate Assays per Kit) * The number of tests per kit can be
More informationab NAD/NADH Assay Kit II (Colorimetric)
Version 1 Last updated 13 November 2017 ab221821 NAD/NADH Assay Kit II (Colorimetric) For the rapid, sensitive and accurate measurement of NAD/NADH in cell lysates. This product is for research use only
More informationHuman anti-deoxyribonuclease B, anti-dnase B ELISA Kit
Human anti-deoxyribonuclease B, anti-dnase B ELISA Kit Catalog No: E0311h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ
More informationOXFORD BIOMEDICAL RESEARCH
Colorimetric Assay for Glutathione Product No. GT 10 For Research Use Only INTRODUCTION Glutathione (gamma-glutamylcysteinylglycine or GSH) is a naturally occuring tripeptide whose nucleophilic and reducing
More informationADH Activity Assay Kit
ADH Activity Assay Kit Catalog Number KA3713 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationHuman von Willebrand Factor cleavingprotease(vwf-cp) ELISA Kit. Catalog No. MBS (96T)
Human von Willebrand Factor cleavingprotease(vwf-cp) ELISA Kit Catalog No. MBS705447 (96T) This immunoassay kit allows for the in vitro quantitative determination of human vwf-cp concentrations in serum,
More informationHuman Paraneoplastic pemphigus (PNP)antibody ELISA Kit. Catalog No. MBS (96 T)
Human Paraneoplastic pemphigus (PNP)antibody ELISA Kit Catalog No. MBS704248 (96 T) This immunoassay kit allows for the in vitro quantitative determination of human Paraneoplastic pemphigus(pnp)antibody
More informationNational Research Council Institute for Ecosystem Study Verbania Pallanza - Italy
Tot-N UV220 pag. 1 National Research Council Institute for Ecosystem Study Verbania Pallanza - Italy Water Chemistry Laboratory Analytical Methods for internal use - http://www.idrolab.ise.cnr.it Gabriele
More informationTex-620-J, Determining Chloride and Sulfate Contents in Soil
Contents in Soil Contents: Section 1 Overview...2 Section 2 Sample Preparation...3 Section 3 Ion Chromatography Method...5 Section 4 Wet Chemical Method...9 Section 5 Archived Versions...15 Texas Department
More informationRat inducible nitric oxide synthase, inos ELISA Kit
Rat inducible nitric oxide synthase, inos ELISA Kit Catalog No: E0837r 96 Tests www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE
More informationLactate Dehydrogenase Assay Kit
Lactate Dehydrogenase Assay Kit Catalog Number KA0878 500 assays Version: 08 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationMethod for Nitrite determination on Low-Range Samples
Method for Nitrite determination on Low-Range Samples 1. PRINCIPLE This method is designed for the determination of nitrite in soil, water and other forms of samples. The sample is prepared offline if
More information#4500-N C, 4500-NO3 F)
UNIVERSITY OF MARYLAND CENTER FOR ENVIRONMENTAL SCIENCE CHESAPEAKE BIOLOGICAL LABORATORY NUTRIENT ANALYTICAL SERVICES LABORATORY 146 Williams St., Solomons MD 20688 http://nasl.cbl.umces.edu/ Standard
More informationFor Research Use Only
L-Ascorbate Assay Kit Kit-0873 Lot. No. (See product label) Specification Description Size Kit Components Materials Required but Not Supplied Preparation L-Ascorbic acid (Vitamin C), an anti-oxidant and
More informationSuccinate (Succinic Acid) Assay Kit (Colorimetric)
Succinate (Succinic Acid) Assay Kit (Colorimetric) Catalog Number KA3955 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background...
More informationLactate Dehydrogenase Assay Kit
Lactate Dehydrogenase Assay Kit Catalog Number KA0878 500 assays Version: 10 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationSpectrophotometric Determination of Ferrocyanide in Effluents
Spectrophotometric Determination of Ferrocyanide in Effluents ECN-0025-1 INTRODUCTION This method is used to determine the concentration of ferrocyanide ion in photoprocessing solution effluents. The ion
More informationCu-Creatinine- Metol system
Quantification of Creatinine in Human Serum using Metol as a Chromogenic Probe Materials and methods 6.1. Reagents 6.1.1. N-methyl-p-aminophenol sulfate N-methyl-p-aminophenol sulfate also denoted as Metol
More informationRayBio Rat TNF-alpha ELISA Kit (For Lysates)
RayBio Rat TNF-alpha ELISA Kit (For Lysates) Catalog #: ELR-TNFa-CL User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationAmendment to the Enforcement Ordinance of the Food Sanitation Law and the Standards and Specifications for Foods and Food Additives.
Amendment to the Enforcement Ordinance of the Food Sanitation Law and the Standards and Specifications for Foods and Food Additives. The government of Japan will designate Polyvinylpyrrolidone as an authorized
More informationLAB. FACTORS INFLUENCING ENZYME ACTIVITY
AP Biology Date LAB. FACTORS INFLUENCING ENZYME ACTIVITY Background Enzymes are biological catalysts capable of speeding up chemical reactions by lowering activation energy. One benefit of enzyme catalysts
More informationD-LACTIC ACID (D-LACTATE) (Rapid)
www.megazyme.com D-LACTIC ACID (D-LACTATE) (Rapid) and L-LACTIC ACID (L-LACTATE) ASSAY PROCEDURES K-DLATE 08/18 (*50 Assays of each per Kit) * The number of tests per kit can be doubled if all volumes
More informationModified Enzymatic Methods for the Determination of L-(+)-Lactic and Pyruvic Acids in Blood
Modified Enzymatic Methods for the Determination of L-(+)-Lactic and Pyruvic Acids in Blood J. F. NEVILLE, JR., M.D., AND R. L. GELDER, CH(ASCP) Department of Surgery, Cardiovascular Laboratory, Upstate
More information