Company Profile of GL Sciences Inc.
|
|
- Hugo Jordan
- 5 years ago
- Views:
Transcription
1 H PLC Sem ina r Introducing Inertsil HPLC Columns How to select HPLC best eluent How to select best column packing UHPLC and FastLC using u particles How to maintain best performance
2 Company Profile of GL Sciences Inc. M ain office 0 branch offices factories R& D center GL U SA Total 470 Employees M ain Office (Tokyo), 70 Em ployees Technical Headquarters (R& D, Customer Support) 00 Employees
3 M ain Products INERT FAMILY IN ERTSIL Colum ns f o r H PLC IN ERTCAP Colum ns for GC IN ERTSEP SPE Cartridge for Sample Preparation
4 Introduction of Inertsil Columns Features and Benefits Inertsil Quality Control Quality comparison among leading companies
5 Inertsil Series H PLC Colum n High Purity, Spherical Silica-gel High Theoretical Plates Highly Uniform ed Particle Size Distribution Lo w Back Pressu re Highly Stable Chem ical Bonding High Durability Extreme End capping Treatment Lower Adsorption Rigorous Inspection Tests (M ore than 0 Tests) Batch- to-batch Reproducibility The biggest feature is W e synthesize the material of the silica- gel and conduct chem ical bonding, inspection and the packing of the column by ourselves. H ighly Stable Excellent Support Continue to Evolve
6 H ighly Pure Base Silica- gel Completely spherical Smooth surface Synthesized from pure ingredients Focused only for chromatography W hole manufacturing process within GLS Inertsil Silica- gel One com petitor's Silica- gel
7 H ighly Pure Base Silica- gel Amount of M etals are inspected by Atom ic Em ission and ICP. M anufacturer's Validation C ertificate Inertsil OD S-3V 5u Lot.N o. VQ5 Atomic Emission ppm Specification R esults Fe < 0 5 Na < 0 4 Al < 0 Ti < < 0.5
8 Influences of M etals
9 Durability of Inertsil Durability Test: Introducing 500 volumes (times) of 0.% TFA against the column volume. Upper Chromatogram: Before Lower Chromatogram: After Phenol Pyridine Time (min) Retention Tim e Volatility (%) Comparison of Durability Test with other manufacturer s column Inertsil ODS Immersed time of TFA (hrs) Column A Column B Column C Column D Column E Column F Column G Time (min)
10 Adsorption of analyte Basic Compounds: Acidic Compounds: Caused by residual hydroxyl group (silanol) on silica surface. Caused by reaction reagent. These reagent includes amine group for the purpose of resistance to basic compounds adsorption. Chelating Compounds: Caused by metal- ions on silica surface. Adsorption mechanism of basic compounds C8 C8 C8 C8 C8 C8 OH OH OH OH OH OH OH OH OH Si Si O Si Si O O O O Silica ODS Si O OH OH Si Si O O O Silica OH OH Si O C Si O End - Capping C OH Si Si O O O Silica O C Si C -Si-O Acidic H hydroxyl group
11 Adsorption comparison Column : mm I.D. Eluent : Acetonitrile / Water=30 / 70 Detection : UV54nm Column Temp. : 40 ) Pyridine ) Phenol of basic compound Inertsil ODS- 3 Company A Company B Company C
12 Adsorption comparison Column : mm I.D. Eluent : M ethanol / Water=30 / 70 Detection : UV54nm Column Temp. : 40 ) Pyridine ) Phenol of basic compound Inertsil ODS- 3 Company A Company B Company C
13 Inertsil Quality Control Scanning Electron M icroscopy Sphericity and surface smoothness of Silica- gel Atom ic Em ission and Inductivity Coupled Plasm a Trace metals N itrogen Adsorption & Laser Ray Particle Analyzer Particle size and its uniform ity Surface area Pore diameter and pore volume 9 Si- NMR Residual Silanol Chromatographic Tests Inertness (Basic, A cid ic, Co m p lexes) Durability (Low ph, H igh ph ) Selectivity (Hydrophobicity, Stereoselectivity, Hydrogen- bonding capacity)
14 Inertsil Quality Control Evaluation Test of Inertness to Basic compounds Time (min) Time (min) Time (min) LOT# VQ 5-43 LOT# VQ5-44 LOT# VQ5-45
15 Inertsil Quality Control Evaluation Test of Inertness to Acidic compounds Column : Inertsil ODS- 3V 50 x 4.6 m m I.D. Eluent : 0.% H 3 PO 4 Detector : UV 0nm Col. Tem p.: 40 Sam ple : ) Form ic acid ) Acetic acid Tim e (m in ) LOT# VQ 5-43 LOT# VQ5-44 LOT# VQ5-45
16 Inertsil Quality Control Evaluation Test of Inertness to Complex compounds Column : Inertsil ODS- 3V 50 x 4.6 m m I.D. Eluent : CH 3 CN / 0.% H 3 PO 4 = 0 / 90 Detector : UV 40nm Col. Tem p.: 40 Sam ple : ) Oxine-Copper ) Caffeine LOT# VQ 5-43 LOT# VQ5-44 LOT# VQ5-45
17 Inertsil Quality Control Evaluation Test of Selectivity. k (n- Amylbenzene) / k (n- Butylbenzene) : the hydrophobicity of the ODS k (Triphenylene) / k (o- Terphenyl) : the stereo-selectivity of the ODS k (Caffeine) / k (Phenol) : the hydrogen-bonding capacity of the ODS 7 ) Uracil ) Caffeine 3) Phenol ) Butylbenzne 5) o- Terphenyl 6) Amylbenzene 7) Triphenylene Time (min)
18 Inertsil Quality Control Evaluation Test of Selectivity. Column : Inertsil ODS- 3V 50 x 4.6 mm I.D. Eluent : CH 3 OH / HO = 80 / 0 Detector : UV 54nm Col. Tem p.: 40 A:Hydrophobicity of the ODS B:Stereoselectivity of the ODS C:Hydrogen- bonding capacity of the ODS A=.47 B=.3 C= 0.49 A=.47 B=.30 C= 0.5 A=.47 B=.30 C= Ti m e (m i n ) 0 0 Ti m e (m i n ) 0 0 Ti m e (m i n ) LOT# VQ 5-68 LOT# VQ5-03 LOT# VQ 5-06
19 Inertsil Quality Control Evaluation Test of residual silanol by 9 Si- NMR. Isolated silanol Si- OH Gem inal silanol Si- (OH) Silica gel Si- (OSi) 4 - O - Si- (CH 3 ) 3 - O - Si- C 8 H 37 Residual silanol Octadecyl Bonding End- Capping
20 Inertsil Quality Control Evaluation Test of residual silanol by 9 Si- NMR. LOT# V Q 5-68 LOT# V Q 5-03 LOT# V Q 5-06
21 Si- N M R spectrum of commercially ODS Residual silanol Residual silanol OD S A OD S B Residual silanol Residual silanol OD S C OD S D
22 Quality Comparison Among Leading Companies High Quality at Reasonable Prices by 30 Year s Experience Execution of whole manufacturing process with reliable and consistent high technologies is essential for the reproducibility of high colum n efficiency resulting in best custom er satisfaction. This is also the only way to reduce the cost which enable us to supply our customer with HPLC colum ns at a reasonable price. Execution of whole manufacturing process Synthesis of base silica- gel from pure ingredients Bonding functional groups on silica- gel m edia Packing silica gel m edia into HPLC column hardware
23 Quality Com parison ~Selectivity~ Colum n Size: mm Eluent :CH3OH / HO = 80 / 0 Flow Rate :.0 m L/ m in Col. Tem p. :40 Detector :UV54 nm Sample Size :5 ul Samples :)Uracil mg/ml )Caffeine 0.04 mg/ml 3)Phenol 0.08 mg/ml 4)n-Butylbenzene. mg/ml 5)o-Terphenyl 0.04 mg/ml 6)n-Amylbenzene.37 mg/ml 7)Triphenylene 0.04 mg/ml Inertsil ODS Column A Column B Column C
24 Quality Com parison ~Selectivity~ Inertsil ODS Column D Column E Column F Column G Column H
25 Quality Com parison ~Selectivity~ Inertsil ODS- 3 Column I Column J Column K Column L Column M
26 Quality Com parison ~Selectivity~ Inertsil ODS- 3 Column N Column O Column P Column Q Column R
27 Quality Com parison ~Selectivity~ Time (min) Inertsil ODS- 3 Column S Column T Column U Column V Column W
28 Quality Com parison ~Selectivity~ Inertsil ODS- 3 Column X Column Y Column Z
29 Quality Com parison ~Pyridine~ Colum n Size: mm Eluent :CH3OH / HO = 30 / 70 Flow Rate :.0 m L/ m in Col. Tem p. :40 Detector :UV54 nm Sam ple Size :4 ul Samples :)Pyridine 0.09 mg/ml )Phenol 0.4 mg/ml Inertsil ODS Column A Column B Column C
30 Quality Com parison ~Pyridine~ Inertsil ODS- 3 Column D Column E Column F Column G Column H
31 Quality Com parison ~Pyridine~ Inertsil ODS- 3 Column I Column J Column K Column L Column M
32 Quality Com parison ~Pyridine~ Inertsil ODS- 3 Column N Column O Column P Column Q Column R
33 Quality Com parison ~Pyridine~ Inertsil ODS- 3 Column S Column T Column U Column V Column W
34 Quality Com parison ~Pyridine~ Inertsil ODS- 3 Column X Column Y Column Z
35 Quality Com parison ~Am inopyridine~ Colum n Size : mm Eluent :CH3OH / 0mM Phosphate Buffer (ph7.6) = 0 / 90 Flow Rate :.0 m L/ m in Col. Tem p. :40 Detector :UV54 nm Sample Size : ul Samples :) 4- Am inopyridine 0. m g/ m L ) 3- Am inopyridine.0 m g/ m L 3) - Am inopyridine.0 m g/ m L Inertsil ODS Column A Column B Column C
36 Quality Com parison ~Am inopyridine~ Inertsil ODS- 3 Column D Column E Column F Column G Column H
37 Quality Com parison ~Am inopyridine~ Inertsil ODS- 3 Column I Column J Column K Column L Column M
38 Quality Com parison ~Am inopyridine~ Inertsil ODS- 3 Column N Column O Column P Column Q Column R
39 Quality Com parison ~Am inopyridine~ Inertsil ODS- 3 Column S Column T Column U Column V Column W
40 Quality Com parison ~Am inopyridine~ Time (min) Inertsil ODS- 3 Column X Column Y Column Z
41 Quality Com parison ~Acids~ Colum n Size : mm Eluent :0.% H3PO4(v/v) Flow Rate :.0 m L/ m in Col. Tem p. :40 Detector :UV0 nm Sample Size :4 ul Samples :) Form ic Acid 0. %(v/ v) ) Acetic Acid 0. %(v/ v) Inertsil ODS Column A Column B Column C
42 Quality Com parison ~Acids~ Inertsil ODS- 3 Column D Column E Column F Column G Column H
43 Quality Com parison ~Acids~ Inertsil ODS- 3 Column I Column J Column K Column L Column M
44 Quality Com parison ~Acids~ Inertsil ODS- 3 Column N Column O Column P Column Q Column R
45 Quality Com parison ~Acids~ Inertsil ODS- 3 Column S Column T Column U Column V Column W
46 Quality Com parison ~Acids~ Inertsil ODS- 3 Column X Column Y Column Z
47 Quality Com parison ~Oxine- copper~ Colum n Size : mm Eluent :CH3CN / 0mM H3PO4 = 0 / 90 Flow Rate :.0 m L/ m in Col. Tem p. :40 Detector :UV54 nm Sam ple Size :.5 ul Samples :) O xine- Copper 0.0 mg/ml ) Caffeine 0.4 mg/ml Inertsil ODS Column A Column B Column C
48 Quality Com parison ~Oxine- copper~ Inertsil ODS- 3 Column D Column E Column F Column G Column H
49 Quality Com parison ~Oxine- copper~ Inertsil ODS- 3 Column I Column J Column K Column L Column M
50 Quality Com parison ~Oxine- copper~ Inertsil ODS- 3 Column N Column O Column P Column Q Column R
51 Quality Com parison ~Oxine- copper~ Inertsil ODS- 3 Column S Column T Column U Column V Column W
52 Quality Com parison ~Oxine- copper~ Inertsil ODS- 3 Column X Column Y Column Z
53 How to select HPLC eluent Select organic solvent for RP- H PLC Reduce running costs using M ethanol Improving the results of chromatogram
54 Organic solvent for RP- H PLC Almost all customers often use acetonitrile. If they can not get good separation... Change column Use buffer or ion- pair Change ph Isocratic Gradient to obtain satisfactory result s... Takes a long time N eed skills and knowledge Difficult to validate W hy are people using acetonitrile?
55 Organic solvent for RP- H PLC Same instrument, column and sample were used. Only changed the type of an organic solvent. 7% CH3CN 80% CH3OH 56% THF Phenol Caffeine o-terphenyl Butylbenzene Triphenylene Am ylbenzene Phenol Caffeine Butylbenzene Am ylbenzene o-terphenyl Triphenylene Phenol Caffeine Triphenylene o-terphenyl Butylbenzene Am ylbenzene
56 Obtaining better results by changing the organic solvent Column : Inertsil ODS- 3 5μm mm I.D. Eluent : Organic / 0.% H 3 PO 4 Flow Rate :.0 m L/ m in Detector : UV 84nm Col. Tem p. : 40 4,5 Sample : ) Sulfamerazine ) Furazolidone 3) Oxolinic acid 4) Sulfadim ethoxine 5) Sulfaquinoxaline 6) N alidixic acid Time (min) 0% CH 3 CN Time (min) 30% CH 3 CN Time (min) 40% CH 3 OH
57 UV spectrum of organic solvents M ethanol Acetonitrile Tetrahydrofuran absorption A B A :H PLC- Grade B:Special- Grade First- Grade absorption A B C A :H PLC- Grade B:Special- Grade C:First- Grade absorption A B A:H PLC- Grade B:Special- Grade First- Grade wavelength(nm) wavelength(nm) wavelength(nm) UV spectrum of HPLC- grade and special- grade are almost in the same level. W hen you use UV- detector, you can obtain satisfactory results even if you use special- grade methanol. UV spectrum of each grade were different. You must be careful when you use low wavelength or highsensitive analysis or gradient analysis. Tetrahydrofuran oxidizes very easily. Special- grade or first- grade THF contains oxidation inhibitor ( 3,5- Di- tert- butyl p- hydroxytoluene). If you use HPLC- grade THF, you must use it as soon as possible.
58 Reduce Running Costs Cost (in Japanese Yen) Solvent S-Grade HPLC-Grade Pressure Toxicity Acetonitrile, / L 3,833 / L Low High Methanol 36 / L 933 / L High Low M ethanol.5 pressure ratio Acetonitrile as.0 when W ater 00% solvents ratio in W ater %
59 Acetonitrile vs.. M ethanol Ability of Separation Eq ual (depends on the character of analyte) Peak Shape Almost equal (depends on the column performance) Running cost M ethanol is better Toxicity M ethanol is better W hy are people not using M ethanol? Because the Back Pressure is too high!!
60 Reduce Running Costs W hen selecting a low- p ressure co lum n Easy to reduce running costs. Can keep yourself healthy. Pressure(kgf/cm) Back pressure of com m ercially available colum ns Inertsil ODS Methanol ratio(%) Conditions Column Size : mm I.D. Flow Rate :.0 m L/ m in Col. Tem p. : 40
61 W hat makes the column back pressure different? It depends on the size of the silica gel uniform ity of the particle size particle shape 5μm Uniform ed Spherical Back pressure = Low 3μm Uniform ed Spherical Back pressure = H igh 5μm Not uniformed Spherical Back pressure= H igh 5μm Uniform ed Irregular Back pressure= H igh
62 W hat makes the column back pressure different? Com petitor Y s silica gel Reasons why Inertsil can offer the lowest back pressure column in the world! Packing - Low ba ck pressure - High theoretical plates - Lot- to- Lot reproducibility Inertsil Packing - H igh ba ck pressure - Low theoretical plates - Poor Lot- to- Lot reproducibility
63 W hy is the particle size distribution different? Every commercial columns have a particle size distribution. But it s distribution width are quite different. Com m ercial silica- gel Inertsil silica- gel Percentage(%) Percentage(%) Particle Size (μm) Particle Size (μm) Almost all column manufactures do not synthesize their silica- gel. They purchase commercial silica- gel from silica- gel specialty manufacture. GL Sciences synthesizes the original silica- gel focused for HPLC columns. As a result, the particle size distribution width is highly controlled.
64 Changing another factor of eluent to improve Separation Peak shape Throughput Buffer concentration Ion- pair reagent concentration Isocratic Gradient Column temperature
65 Improving Peak Shape Effect of Buffer concentration Eluent : CH 3 CN / Buffer = / 88 Sample : ) Thiam in ) Riboflavin mm N ah PO 4 0mM NaH PO 4 00mM NaH PO 4 It is efficient to use high concentration buffer to improve the peak shape. H owever, it will cause the colum n to deteriorate as well. W hen analyzing a strong adsorptive compound, it is important to select a column that has high inertness but durability too.
66 Improving Separation Effect of ion- pair reagent concentration Ion-pair reagent: Heptafluoro butyric acid % 0.5 % Sam ple.tolazoline.prazo sin 3.Yohim bin 4.Phentolamine 5.Labetalol 0.0 %
67 Improving Throughput Effect of Gradient elution CH 3CN / H O = 50 / 50 CH 3 CN / H O = 40 / 60 Gradient
68 Improving Throughput Effect of Column Temperature Inertsil ODS- 3 3μm ; m m I.D. ; 70% CH 3 CN ;.5 ml/min Press:7M pa N(No.5):7985 Press:M pa N(No.5):3643 Press:0M pa N(No.5):349 Press:8M pa N(No.5):50
69 How to select Column packing Difference and features of ODS Colum n line-up except ODS Reduce running costs by Scale- down Im prove Rs using sm all particle size colum n
70 Differences and features of ODS The first choice column should be an Octadecyl groups bonded (ODS) column. There are various ODS columns that offers different characteristics? Base Silica Physical Properties and Chem ical M odification of Inertsil ODS Series Silica-gel Chemical Treatment Column Surface Carbon Size Pore Size Purity Area Loading ODS Type End-Cap Inertsil ODS-3 3,4,5μm 450 m /g 00A % 5% Monomeric Well Inertsil ODS-P 5μm 450 m /g 00A % 9% Polymeric None Inertsil ODS-EP 5μm 450 m /g 00A % 9% Embedded None Inertsil ODS-SP 5μm 450 m /g 00A % 8.5% Monomeric Well Inertsil ODS- 5μm 30 m /g 50A % 8.5% Intermediate Well Inertsil ODS-80A 5μm 450 m /g 80A 99.99% 7.5% Monomeric Well
71 M onomeric ODS vs. Polym eric ODS Planar compounds M onomeric ODS Silica- gel surface C8 group Planar compounds Non- planar compounds Polym eric ODS
72 M olecular Recognition of ODS ) Benzo[a]pyrene (BaP) ) Tetrabenzonaphthalene (TBN ) Analytical conditions Column : 50 x 4.6 mm I.D. Eluent : CH 3 CN / H O = 85 / 5 Flow Rate :.0 m L/ m in Detector : UV 54nm Col. Tem p.: 30 Selectivity(α ) α =k TBN / k Bap 3) Phenanthro[3,4- c]phenanthrene (PhPh) α.7 monomeric.7>α>.0 intermediate α.0 polym eric
73 M olecular Recognition of ODS Inertsil ODS- P Inertsil ODS- Inertsil ODS- 3 α = 0.7 α =.65 α =.5 TBN PhPh BaP + PhPh TBN BaP PhPh TBN BaP Time (min) Time (min) Time (min) Polym eric Intermediate M onomeric
74 Application of PAH s. Naphthalene. Acenaphthylene 3. Biphenyl 4. - M ethylnaphthalene 5. - M ethylnaphthalene 6. Acenaphthene 7. Fluorene 8. Phenanthrene 9. Anthracene 0. Fluoranthene. Pyren e. Ben zo - [a]- anthracene 3. Chrycene 4. Ben zo - [ j]- fluoranthene 5. Ben zo - [e]- pyrene 6. Ben zo - [b]- fluoranthene 7. Ben zo - [k]- fluoranthene 8. Ben zo - [a]- pyrene 9. D ib en zo - [a,h ]- anthracene 0. Ben zo - [g,h,i]- perylene. Indeno- [,,3- cd]pyrene Analytical Conditions Colum n Size : m m I.D. 5μm Eluent : A = H O ; B= CH 3O H A/B= 0/80(7min Hold) ---0min --- 0/00 Flo w rat e : ml/min Detector : UV 54nm Col. Temp. : 40 Sam ple size : 5 μl (each 00ng / ml) Time (min) Time (min) Inertsil ODS- 3 (monomeric) Inertsil ODS- P (polym eric)
75 Application of Vitam in D & D3 Analytical Conditions Colum n size : 50mm x 4.6mm I.D Eluent : Acetonitrile / M ethanol (75:5) Flo w rat e :.0m L/ m in Te m p. : 30 Detection : UV 80nm Sam p les :. Vitamin D (450ng). Vitamin D3 (50ng) H O H C C H 3 H 3 C Vitam in D C H 3 C H 3 C H 3 H O H C H 3 C H 3 C Vitam in D3 C H 3 C H Inertsil ODS- 3 (monomeric) Inertsil ODS- P (polym eric)
76 Application of Glycyrrhizin Analytical methods written in the Japanese Pharmacopoeia Column I.D.: Column length: Column packing: Flow Rate: 4.0~6.0mm 5~5cm ODS (The elution pattern shall be from Glycyrrhizin to p - Hydroxybenzoate propyl, which is an internal standard. Each peak shall be completely separated.) Adjust it until the retention tim e of Glycyrrhizin is about 0m in. Column size : 50 x 4.6 mm I.D Eluent : H O / Acetic acid / CH 3 CN =560 / 40 / 400 Flow rate : ODS-3 0.8mL/min HO ODS-P 0.4mL/min HO Col. Tem p. : 5 Detector : UV 54nm Sample :. Glycyrrhizin (750ng). p- hydroxy benzoic acid propyl (50ng) CO O H O H O O HO H O CH 3 O O H O H CH 3 CO O H H CH 3 CH 3 CH 3 CH 3 CH 3 CO O H Structural formula of Glycyrrhizin
77 Application of Glycyrrhizin Column size : 50 x 4.6 mm I.D Eluent : H O / Acetic acid / CH 3 CN = 560 / 40 / 400 Col. Tem p. : 5 Detector : UV 54nm Sample : ) Glycyrrhizin (750ng) ) p- Hydroxy benzoic acid propyl (50ng) Flow rate = 0.4 ml/min Flow rate = 0.8 ml/min Time (min) Inertsil ODS- P (Polym eric) Time (min) Inertsil ODS- 3 (M onomeric)
78 Effect of Silanol Good End capping No End capping Treatment Polym eric ODS Time (min) Time (min) Good End capping M onomeric ODS Poor End capping No End capping Treatment Time (min) Time (min) Time (min)
79 Selectivity of Embedded ODS Analytical Conditions Column : mm I.D. Eluent : CH 3 CN / H O = 70 / 30 Flow Rate :.0 ml/min Detector : UV 54nm Sam ple : ) Ethylbenzene ) N aphthalene 3 ) n- Propylbenzene 4 ) n- Butylbenzene 5 ) Anthracene Inertsil ODS- EP =Embedded Inertsil ODS Inertsil C
80 79 GL Sciences Application of Antibacterial Column : mm I.D. Eluent : Organic / 0.% H 3 PO 4 Flow Rate :.0 m L/ m in Detector : UV 84nm Col. Tem p. : 40 4,5 Sample : ) Sulfamerazine ) Furazolidone 3) Oxolinic acid 4) Sulfadim ethoxine 5) Sulfaquinoxaline 6) N alidixic acid 0% CH 3 CN 3 30% CH 3 CN 0% CH 3 CN Time (min) Time (min) Time (min) Inertsil ODS- 3 Inertsil ODS- EP
81 80 GL Sciences W eakness of Embedded ODS Ordinary embedded ODS are bonded with amine polar groups. Therefore, acidic com pounds cannot be analyzed since it will be adsorbed to the embedded amine groups. However, our Inertsil ODS- EP does not use an amine polar group. As a result, it can also be used for analyzing acidic compounds. Form ic acid Acetic acid Form ic acid + Acetic acid Acetic acid Form ic acid Acetic acid Form ic acid 0 4 Time (min) Inertsil ODS- EP 0 4 Time (min) Column A 0 4 Time (min) Column B 0 4 Time (min) Column C
82 Low Carb on Loading O D S Features of Low Carbon Loading ODS Retaining hydrophilic compounds without excessive retention of hydrophobic compounds. Elut es sharp p eaks. Compatible with 00% aqueous eluents. 3 Inertsil ODS- SP =Low Carbon Loading 4 Analytical Conditions Column Size : 50x4.6 mm I.D. Eluent : A) Acetonitrile B) 0mM NaHPO4 (ph3.0) (A/B) = (70/30) Flow rate :.0 m L/ m in Col. Tem p. : 40 Detector : UV 80 nm Injection Vol. : 5μL ) Phenol (50ppm) ) Bisphenol- A (30ppm) 3) 4- Octylphenol (00ppm) 4) 4- Nonylphenol (00ppm) (in CH 3 OH) Time (min) Inertsil ODS- 3 =General ODS Time (min) 3 4
83 Features of Low Carbon Loading ODS Retention for hydrophilic compounds are about the same as an ordinary ODS! Analytical Conditions Column Size : 50x4.6 mm I.D. Eluent : A) M ethanol B) 0mM NaHPO4 (A/B) = (0/90)-30min-(50/50) Flow Rate :.0 m L/ m in Col. Tem p. : 40 Detector : UV at 80 nm Injection Vol. : 0μL (each mg/ L) ) Gallocatechin (GC) ) Epigallocatechin (EGC) 3) Catechin (C) 4) Epigallocatechin Gallate (EGCg) 5) Epicatechin (EC) 6) Gallocatechin Gallate (GCg) 7) Epicatechin Gallate (ECg) 8) Catechin Gallate (Cg) Inertsil ODS- SP =Low Carbon Loading Inertsil ODS- 3 =General ODS Time (min)
84 Advantages of Low Carbon Loading ODS W hen increasing the concentration of an organic solvent to reduce the analysis time... Possibility in deteriorating the separation. Im purity Im purity Target compound Target compound Target compound 3 Im purity Enlarged view Target compound Time (min) Time (min) 30% Organic 40% Organic
85 Advantages of Low Carbon Loading ODS Possible to reduce the analysis time when using a Low Carbon Loading ODS without changing the eluent conditions. Of course, the separation will not be deteriorated. Im purity Target compound Target compound Target compound 3 Im purity Target compound Time (min) Inertsil ODS- 3 =General ODS Time (min) Inertsil ODS- SP =Low Carbon Loading
86 Advantages of Low Carbon Loading ODS Analytical Conditions Column: 5um, 50 x 4.6mmI.D. M obile Phase: CH 3CN / H O = 5/95 (5min hold) (7min) 00/0 (5min hold), v/v Flow Rate:.0mL/min Tem p.: 40C Detection: UV5nm ) M ethamidophos ) Acephate A highly uniformed low carbon loading ODS enables shorter equilibration time for gradient analyses and can be used for high- throughputs. Of course, the time for purging and cleaning the column can be reduced as well in an isocratic. Fluctuations in retention time are shown on Column C and Column D. On the other hand, Inertsil ODS- Sprint shows a complete equilibration after 0mins with stable rententivity.
87 Application of Food Colorants Analytical Condition Time (min) 8 9 Column : Inertsil ODS- SP 5um 50X4.6 mm I.D. M obile Phase: A) Acetonitrile B) 0mM Phosphate buffer (ph6.9) (A/B) = (0/90)-50min-(35/65) Flow Rate :.0 m L/ m in Column Temp.: 40C Detector : UV at 70nm Sam ple Vol.: 0uL ) Acid Yellow N o.4 7.6mg/L ) Acid Red No. 3.8mg/L 3) Acid Blue N o. 7.6mg/L 4) Acid Red No.0 3.8mg/L 5) Acid Yellow N o.5 5.3mg/L 6) Uranine 3.8mg/L ) Acid Red No mg/L 8) Ponceau R 7.6mg/L 9) Ponceau SX 5.3mg/L 0) Orange 5.3mg/L ) Acid Green N o.3 3.0mg/L ) Acid Blue N o. 3.0mg/L 3) Acid Red No.3 5.3mg/L 4) Azul Blue VX 3.0mg/L 5) Orange 7.6mg/L 6) Acid Red No mg/L
88 Comparison among Leading Companies Inertsil ODS- Sprint This m ethod is em ployed by one of industry leaders to investigate peak shape of basic compounds. Amitriptyline, basic prove was analyzed under the most demanding mobile phase condition at ph 7.0 and USP tailing factor was m easured. Iner t sil columns show very good peak shapes for such significant basic compound. Comparison of Tailing Factor Inertsil ODS-3 Inertsil ODS-Sprint Sunfire Inertsil ODS-3 4um Symmetry Xterra Ec lips e Colum n Tailing Factor Inerts il O D S Inertsil ODS-Sprint.06 Sunfire.06 Inertsil ODS-3 4um.07 Symmetry. Xterra.3 E c lips e.8 Tailing Factor
89 Comparison among Leading Companies mau m Volt Inertsil ODS-Sprint Analytical Condition Eluent: 0mM Phosphoric Acid Buffer (ph7.0) / M eoh = 0/80 (v/v) Flow:.0m L/ m in Detect: UV54nm Oven: 40C Sample: 0uL- inject m Volt mau Zorbax Eclipse XDB- C Uracil ug/ml.propranolol 99ug/mL 3.n-Butyl paraben ug/ml 4.N aphthalene 37ug/mL 5.Acenaphthene 50ug/mL 6.Am itryptyline 30ug/mL
90 Quality Comparison Among Leading Companies Pyridine Test for Base Deactivation m Volt Inertsil ODS- SP m Volt mau Inertsil ODS m Volt Sym m etry C8 Pyridine tailing due to poor end- capping SunFire C8 Pyridine tailing due to poor end-capping. m Volt Pyridine tailing due to poor end- capping. Column Length: 4.6X50m m 5um Eluent CH3OH / HO = 30 / 70 Flo w Rat e:.0 m L/ m in Co l. Tem p.: 40C Detector:: UV54nm Sample Volume: 4uL Sam p les: ) Pyridine 0.09 mg/ml ) Phenol 0.4 m g/ m L XTerra M S C Atlantis dc Zorbax Eclipse XDB- C8
91 Quality Comparison Among Leading Companies Aminopyridine Test for Base Deactivation m Volt Inertsil ODS- SP 3 3 m mau Volt 0 00 m Volt Inertsil ODS m Volt All peaks are adsorbed Sym m etry C8 All peaks are adsorbed SunFire C8 3 Column Length: 4.6X50m m 5um Eluent CH3OH / 0m M Phosphate Buffer (ph7.6) 0:90 Flo w Rat e:.0 m L/ m in Co l. Tem p.: 40C Detector:: UV54nm Sample Volume: 4uL Sam p les: ) 4- Am inopyridine 0.m g/ m L ) 3- Aminopyridine.0mg/mL 3) - Aminopyridine.0mg/mL XTerra M S C8 0 0 Atlantis dc8 0 0 Zorbax Eclipse XDB- C8
92 Quality Comparison Among Leading Companies Oxine- copper Test for Silica Purity m Volt mau m Volt Oxine- copper shows tailing Sym m etry C8 0 0 SunFire C8 Inertsil ODS- SP Inertsil ODS- 3 m Volt Oxine- copper shows tailing. Oxine- copper shows tailing. Column Length: Eluent Flo w Rat e: Co l. Tem p.: Detector:: Sample Volume: Sam p les: 4.6X50m m 5um CH3OH / 0m M H3PO4 (0/ 90, w:w).0 m L/ m in 40C UV54nm.5uL ) Oxine- copper 0.0 mg/ml ) Caffeine 0.4 m g/ m L XTerra M S C8 0 0 Atlantis dc8 0 0 Zorbax Eclipse XDB- C8
93 Quality Comparison Among Leading Companies Carboxylic Acid Test m Volt m mau Volt m Volt All peaks are adsorbed. All peaks are adsorbed Inertsil ODS- SP Inertsil ODS Sym m etry C SunFire C8 All peaks are adsorbed. m Volt Form ic acid is adsorbed. Column Length: 4.6X50m m 5um Mobile Phase: 0.% H3PO4(v/ v) Flo w Rat e:.0 m L/ m in Co l. Tem p.: 40C Detector: UV0nm Sample Volume: 4uL Sam p les: ) Form ic Acid 0. %(v/ v) ) Acetic Acid 0. %(v/ v) XTerra M S C Atlantis dc Zorbax Eclipse XDB- C8
94 Other Column Lineups For low molecular compounds Inertsil C8-3 Inertsil Ph- 3 Inertsil CN - 3 Inertsil N H Inertsil AX Inertsil H ilic Inertsil SIL- 00A Inertsil CX Inertsil Diol For high molecular compounds Inertsil W P300- C8 Inertsil W P300- C8 Inertsil W P300- C4 Inertsil W P300- SIL Inertsil W P300- Diol Fo r sp ecial use Inertsil Sulfa C8 Inertsil Peptides C8 (Designed for analysis of Sulfa Drugs) (Designed for analysis of Peptides)
95 Analysis tim e com parison of ODS- 3 vs. C Column : 50 x 4.6 mm I.D. Eluent : CH 3 CN / H O = 70 / 30 Flow rate :.0 ml/min Detector : UV 54nm Co l. Tem p. : 40 Sam ple : ) N aphthalene ) Ethylbenzene 3) Propylbenzene 4) Anthracene 5) Butylbenzene Inertsil ODS Inertsil C8-3
96 Application of Fat- soluble Vitam ins Column : Inertsil C mm I.D. Eluent : CH 3 OH / H O = 95 / 5 Flow Rate :.0 m L/ m in Detector : UV 80nm Col. Tem p. : 40 ) Vitam in K3 ) Vitam in D3 3) Vitam in E ) Vitamin E acetate 5) Vitam in K Time (min)
97 A nalysis co m p ariso n of ODS- 3 vs. Ph- 3 + Column Eluent : 50 x 4.6 mm I.D. : O D S 90% CH 3 OH Ph 70% CH 3 OH Flow rate :.0 ml/min Detector : UV 54nm Co l. Tem p : 30 Sam ple : ) Fluorene ) Phenanthrene Fluorene Time (min) Time (min) Inertsil ODS- 3 Inertsil Ph- 3 Phenanthrene
98 Feature of Inertsil Ph- 3 Ordinary phenyl columns are bonded with alkylphenyl groups. For our Inertsil Ph- 3, a phenyl group is directly bonded to the silica- gel to maximize the effect of an electron affinity. Inertsil Ph- 3 Si Time (min) Inertsil ODS Time (min) Si ( CH ) n Time (min) Analytical Conditions Column Size:4.6m m I.D. 50mm Eluent :60% Acetonitrile (Ph Column) 75% Acetonitrile (ODS column) Flow Rate:.0m L/ m in Col. Tem p. :40 Detector :UV 0nm Samples ) Flucythrinate ) Cyhalothrin 3) Deltamethrin 4) Fluvalinate 5) Tralomethrin (two isom ers)
99 A nalysis co m p ariso n of ODS- 3 vs. CN - 3 Inertsil CN - 3 Inertsil ODS Analysis of Organic acids Column : m m I.D. 5μm Eluent : 0mM Phosphate buffer (ph4.0) Flow Rate :.0 m L/ m in Detector : UV 0nm Col. Tem p. : 40 Sample : asparagine aspartic acid H N COOH C H CH CONH H N COOH C H CH COOH 3 fum aric acid 4 m aleic acid H HOOC C C COOH H H HOOC C C H COOH
100 A nalysis co m p ariso n of ODS- 3 vs. CN - 3 Column : m m I.D. 5μm Flow Rate :.0 m L/ m in Detector : UV 360nm Col. Tem p. : 40 A nalysis of D N PH - Aldehydes Sample : ) Formaldehyde ) Acetaldehyde 3) Propionaldehyde 4) n- Butylaldehyde 5 ) iso - Butyraldehyde Time (min) Inertsil ODS- 3 Eluent : CH 3 CN / H O = 60 / Time (min) Inertsil CN 3 Eluent : CH Cl / Hexane = 0 / 90
101 W hat is HILIC? HILIC = ( H ydrophilic Interaction Chromatography ) HILIC is a variation of normal phase mode where a high concentration of nonpolar solvent is used in a mobile phase and an ideal method for separation and retaining highly polar analytes unretained by traditional reversed- phase mode. Applications for HILIC mode Am ino acids Water-soluble vitam ins Saccharides Drug m etabolites 3 4 0% CH 3 CN 60% CH 3 CN 90% CH 3 CN Column : Inertsil HILIC 5μm 50 x 3.0 mm I.D. Eluent : 0.%TFA in % CH 3 CN Flow rate : 0.4 m L/ m in Detector : UV 54nm Col. Tem p.: 40 Sam ple : ) Thiam ine 8μg/ ml ) N icotinam ide 8μg/ ml 3) L- Ascorbic Acid 80μg/ ml 4) Riboflavin 8μg/ ml
102 Application of Oligosaccharides 3 4 Analytical Conditions Column : Inertsil HILIC 5um mm I.D. Eluent : Acetonitrile / HO = 85/5 (w/w) Detection : RI Col. Tem p.: 40 Flow Rate:.0 m l/ m in Samples : ) M alt ose ) M altotriose 3) M altotetraose 4) M altopentaose 5) M altohexaose 6) M altoheptaose Time (min)
103 Scale- Down of column I.D. Advantages Amount of Eluent and waste solution can be reduced by setting a low flow rate. Since the sensitivity will increase the usage amount of sample can be reduced or trace amount of compounds can be analyzed. Can reduce the labor hours of making the eluent composition and sample preparation procedures. Improving the working environment and reduce the harmful effect to nature. ISO400: Save Energy and Resources!!! Reduce Amount of Eluent with Smaller ID Inertsil Columns!!! Weakness The influence of dead volume will deteriorate the peak shape and separation.
104 Example of Scale- Down Column :Inertsil ODS- 3 5μm 50mm I.D. 4.6 m m Flow.0 m L/ m in I.D. 3.0 m m Flow 0.4 m L/ m in I.D.. m m Flow 0. m L/ m in 5mV Sensitivity Consumption of eluent Liquid waste volum e Separation twofold 60% cut 60% cut Com plete fourfold 80% cut 80% cut Incom plete
105 Optim izing the colum n length M obile Phase: Column Temp.: 65% Acetonitrile 40C To improve separation 5μm;50 mm Theoretical Plate 3,000; 6M Pa 3μm;50 mm Theoretical Plate 0,000: M Pa 3μm;50 mm Theoretical Plate 30,000; 8M Pa 4μm;50 mm Theoretical Plate 7,000; 8.5M Pa Inertsil ODS- 3 5μm;50 mm Theoretical Plate 3,000;3.5M Pa
106 Performance comparison between various Particle Sizes Theoretical Plates of N umbers Back Pressure 35,000 30,000 5,000 3,500 7,00 30, M Pa 6.8 M Pa 3.8 M Pa 0,000 5μm 4μm 3μm 0 5μm 4μm 3μm The theoretical plates are inversely proportional to the particle size The Pressure is inversely proportional to the sq uare of the particle size
107 Features of 4um column Low Back Pressure!(/ the back pressure of 3um column) Particle size 5μm 4μm 3μm Column length 50 mm 50 mm 50 mm 50 mm Pressure 9.3 M Pa 3.5 M Pa 9.0 M Pa 7.5 M Pa Higher Theoretical Plates of Numbers! 50% M ethanol ; 4.6mm ;.0mL/min (Increase in column efficiency by over 5% over 5um column) Particle size 5μm 4μm 3μm Column length 50 mm 50 mm 50 mm 50 mm Theoretical plate 3,5 7,06 30,484 0,998 65% Acetonitrile ; 4.6mm ;.0mL/min ; Naphthalene Extended Column Life Time! (Using the same filter of a 5um column) The column durability is the same as a 5um column.
108 Optim izing the colum n length Inertsil ODS- 3 5μm;50 mm Theoretical Plate 3,000;3.5M Pa M obile Phase: Column Temp.: Analysis Tim e: 65% Acetonitrile 40C 5 minutes To shorten the analysis tim e 3μm;00 mm Theoretical Plate,500; 7.5M Pa 3μm;75 mm Theoretical Plate 9,000: 5.5M Pa 0mins Increased flow rate folds 7.5mins 3μm;00 mm Theoretical Plate,000; 5M Pa 3μm;75 mm Theoretical Plate 8,500; M Pa 5m ins 3.8mins 4μm;00 mm Theoretical Plate,000: 5.5M Pa 0mins 4μm;00 mm Theoretical Plate 9,000; M Pa 5m ins To reduce more analysis time
109 Advantages of small particle size column Inertsil ODS- 3 5μm mm I.D. CH 3 CN / H O = 70 / 30.0 ml/min 40 Pressure =.9 M Pa Particle Size Column Size Eluent Flow Rate Co l. Tem p. Sam ple Ethylbenzene Propylbenzene Butylbenzene iso - Amylbenzene n- Amylbenzene Inertsil ODS- 3 3μm mm I.D. CH 3 CN / H O = 70 / 30.5 ml/min 40 Pressure =0.5 M Pa Time (min) Analysis Tim e Consumption of eluent Separation Pressure Time (min) /3 45% less Com plete Very H igh
110 Van Deemter Plot of HETP vs. Flow Rate Column: 50 X.mmI.D.
111 Comparison of um vs. 5um
112 Pressu re vs. Flow Rate
113 Exam ple of H igh- Speed Separation Analytical Condition Sam ple Column: Inertsil ODS- 3. Gallic acid (0.7mg/mL) Eluent: A: Acetonitrile. Vanillic acid (0.7mg/mL) B: 0.% Phosphoric Acid `. Vanillin (Im purity of Vanillic acid) A/B=5/85 3. p - Coum aric acid (0.7mg/mL) Flow Rate: 0.5m L/ m in.,.0m L/ m in. 4. Ferulic acid (0.7mg/mL) Column Temperature: 40C 5. m - Coum aric acid (0.7mg/mL) Detector: PDA 75nm Injection Volum e: 0uL (4.6m m I.D.), 5uL (3.0m m I.D.) Analysis Time: 9 mins Result s: All peaks were well separated.
114 Exam ple of H igh- Speed Separation - Shorten the column length - Changing the packing material to a 3um Analysis Time: 4 mins Result s: Obtained the sam e resolution with shorter analysis tim e.
115 Exam ple of H igh- Speed Separation - Shorten the column length - Using the same flow rate Analysis Time: 3.5 mins Result s: The analysis time was shortened to 3.5 minutes. However, peak ` and 3 were not separated.
116 Exam ple of H igh- Speed Separation - Changing the packing material to a um Analysis Time: 3.5 mins Result s: The analysis time was shortened to 3.5 minutes. In addition, complete separation between peak ` and 3 is obtained.
117 H igh- Speed Separation Inertsil ODS- 3 um Base Silica Physical Properties and Chem ical M odification Base Silica: High Purity Silica Gel % Surface Area: 450 m /g Pore Size: 00A Pore Volume:.05 ml/g Bonded Phase: Octadecyl Groups Carbon Loading: 5% End capped: Yes Column Sizes: 50. mm I.D mm I.D. M aximum Operating Pressure: 50 M Pa Guaranteed Theoretical Plates: 60,000/m (GLS Standard Column Performance Test)
118 Features of Inertsil ODS- 3 um Advanced classification technologies for sizing silica gels realized an ideal balance between theoretical plate number and column back pressure enabling high throughput analysis in the existing standard HPLC system. Fine particles are thoroughly eliminated and proper mesh size is employed, which result in less column clogging problems. Customers who are currently using Inertsil ODS- 3 5um, 4um or 3um in their analyses can now simply readily achieve High- Speed Separation without changing the analytical conditions. A newly- developed column joint is used to minimize the dead- volum e, which is an ideal design for high throughput analyses, resulting in high/sharp peak shapes for those fast eluted samples. * M aximal performance can be obtained at the following flow rate:.m m I.D.: From 0.4 to 0.6 ml/min 3.0m m I.D.: From 0.8 to. ml/min
119 Durability Test -Colum n Clogging- Confirmed no column clogging or no increase of column back pressure after a,000 times injection of pretreated 5uL serum. Before Test Pretreated serum: Using an human serum supernatant, which was centrifuged mixed with commercially available human serum adding equal volumes of methanol. The pressure fluctuation of the column Time (min) After Test Time (min)
120 Durability Test -Pressure Durability- - The analytical condition was purposely set to have the maximum pressure reach at 50M Pa. - The test was repeatedly conducted and observed no variation in retention and peak shape. - Instead of an isocratic, a gradient condition was employed since it generates more pressure fluctuation resulting in more rigorous durability test. Analytical Conditions M obile Phase: Water00% 0min M ethanol00% Flow Rate: 0.5 m L/ m in M aximum Pressure: 50M Pa (At W ater/ M ethanol = 50/ 50) Sample: Alkylphenol 6 8 Time (min) 6 8 Time (min) 6 8 Time (min) st Injection After 00 Injections After 500 Injections
121 Inertsil ODS- 3 um Instruction for Use W hen using an ultra high pressure specification instrument, please be careful with the fitting type. To m axim ize colum n life, operate at pressures within 50M Pa (approx. 7,00psi). To maximize the performance of the column, extreme care should be taken on the internal diameter of piping and detector cell. The below mentioned Seal Tight Fitting (Upchurch) can also be tightened by hand, however, leakage may be observed due to tolerance of an outer diameter of the piping or worn piping. Use stainless steel male nut and ferrule when analyzing precious samples. Please be careful with the setting of response speed of detector and retrieving interval on the data processor in a high throughput analysis. Description Cat.No. Seal Tight Fitting, Short Nut, 0PCS/PACK, P/N F-95X (Upchurch) * Seal Tight Fitting, Long Nut, 0PCS/PACK, P/N F-96X (Upchurch) *
122 H igh-speed Separation Columns Inertsil C8-3 μm The same base silica synthesizing and bonding technologies used for Inertsil ODS- 3 are also employed for Inertsil C8-3 um. The difference between these two phases is just the length of the hydrocarbon ligands. This enables faster analyses to be performed by converting from an Inertsil ODS- 3 column to an Inertsil C8-3 column without changing the separation pattern. Base Silica Physical Properties and Chem ical M odification Inertsil ODS- 3 um, mm I.D. Inertsil C8-3 um, mm I.D. 3 4 Base Silica: High Purity Silica Gel % Particle Size: um Surface Area: 450 m/g Pore Size: 00A Pore Volum e:.05 ml/g Bonded Phase: Octadecyl Groups Carbon Loading: 9% End capped: Yes Conditions Syst em : GL system Columns: Inertsil ODS- 3, C8-3 Eluent: A : CH 3 CN B: H O A/B = 50/50, v/v Flow rate:.0 ml/min Detector: PDA 60 nm In j. Vo l.: 5 ml Sam ple:. Naphthalene. Ethylbenzene 3. n - Propylbenzene 4. Anthracene 5. n - butylbenzene Ordering Information Length / I.D..mmI.D. 3.0mmI.D. 30mm mm List Price US$ The shorter alkyl group a functional group has, the less hydrophobic interaction it has. Therefore, the retentivity of C8-3 is weaker than ODS- 3.
123 H igh-speed Separation Columns Inertsil Ph- 3 μm Ordinary phenyl columns are bonded with alkylphenyl groups. For our Inertsil Ph- 3, phenyl groups are directly bonded the silica- gel to m axim ize the effect of an electron affinity. Inertsil Ph- 3 shows inherent inertness and unique selectivity. Base Silica Physical Properties and Chem ical M odification Inertsil C8-3 um, mm I.D. Inertsil Ph- 3 um, mm I.D Base Silica: High Purity Silica Gel % Particle Size: um Surface Area: 450 m/g Pore Size: 00A Pore Volum e:.05 ml/g Bonded Phase: Phenyl Groups Carbon Loading: 9.5% End capped: No 5 Conditions Syst em : GL system Columns: Inertsil C8-3, Ph - 3 Eluent: A : CH 3 CN B: H O A/B = 50/50, v/v Flow rate:.0 ml/min Detector: PDA 60 nm In j. Vo l.: 5 ml Sam ple:. Naphthalene. Ethylbenzene 3. n - Propylbenzene 4. Anthracene 5. n - butylbenzene Inertsil Ph- 3 not only m akes the analysis tim e shorter than C8-3, but also provides an unique retention for No. and No.4, which offers a different separation pattern. Ordering Information Length / I.D..mmI.D. 3.0mmI.D. 30mm mm List Price US$
124 How to Maintain Column perform a nce N ecessity of cleaning the water for eluent Using guard column How to wash and store columns
125 Cleaning Water for eluent Im purities (organic compounds or bacteria ) in water ODS column 00% Water 60min hold - 0min 00% Acetonitrile UV 0nm (Retain the impurity) (Eluted) <Deionnization+Ultraviolet oxidation+ M em brane filtration> <Deionization+ Activated carbon filtration+ M em brane filtration > <Deionization+ Distillation> <Deionization>
126 Retained im purities on the colum n Flow Accumulation of stains (im purities in water) Silica- gels became hard Resulting in increase in back pressure, deterioration of separation, poor peak shape and unknown peaks.
127 Colum n for rem oving Im purities Pre- clean ORG rem oves organic im purities from water. This is placed in the line between pump and injector. Seal scum of pumping system also can be retained on Pre-clean ORG before flowing into analysis column.
128 Another way of cleaning eluent By using the Solid Phase Extraction procedure, the phenomena of base line drifts or ghost peaks can be prevented by filtering the mobile phase. Reference Title: M obile-phase Cleanup Using Solid-Phase Extraction Disks Journal Title: LCGC N O RTH AM ERICA ( Volume, N umber, Year: V olume, Number, 003 Page: P68~78 Author: M.C.Ringo, J.R. Allen, D.M. M attocks, (GlaxoSm ithkline)
129 Recommendation of Guard Column The purpose of using a Guard Column Removing high lipophilic impurities from the sample (for reversed phase mode) Removing high polarity impurities from the sample (for normal phase mode) Rem oving im purities from the sam ple The head of the column view when using a guard column The head of the column view without a guard column
130 Recommendation of Guard Column Concerns when using a Guard Column The separation efficiency was poor The retention time was late Possibility that a different packing material is used for the guard column All of our Guard Columns are using the absolutely same packing m aterials that are packed in the analytical colum ns. Therefore, please feel safe on using our guard columns. W ithout guard column W ith Cartridge guard column E W ith GL cart Cartridge Guard Column E GL- Cart Rt. = 6.5 min T.P. = 3,693 As. =.03 Rt. = 6.48 min T.P. = 3,464 As. =.05 Rt. = 6.49 min T.P. =,75 As. =.07
131 Before using a N ew column Inertsil series are shipped with the solvents as below for the final test of the column. Before using the column, please confirm miscibility to the below solvents that you will be using as a mobile phase. W hen using a mobile phase that is non-miscible to the below solvents, please purge the column with Isopropanol by 5 to 0 volumes of the column volume. After purging the column with Isopropanol, substitute with the mobile phase. Colum n ODS, C8, C4, Ph, HILIC CN, N H, Diol, SIL Shipping solvents Acetonitrile / W ater Hexane / Ethanol Solvents that cannot be mixed with the shipping solvent Hexane, Heptane, Isooctane, Chloroform, Dichloromethane etc Water, M ethanol, Acetonitrile etc AX, CX M ethanol Hexane, Heptane, Cyclohexane, Isooctane, etc
132 Daily maintenance of the column Eluent with acids, buffer, ion- pair At first you must wash the column using same organic solvent and ratio(%) that was used as an eluent without any additives. Eluent without additives Example: Eluent = 0.% H3PO4 in 35% M ethanol W ash so lvent = 35% M ethanol Wash column using 00% organic. = same as an eluent Store column with eluent. (without additives) W hen using a gradient elution, use 30 ~ 50% of organic to store column.
133 How to wash column W hen is the best timing to wash the column? W hen you observe fluctuation in retention time, increase in pressure, deterioration of separation, and poor peak shape. In case of using reverse phase mode including HILIC mode In case of using normal phase mode To remove polar compounds ) Water ) M ethanol 3) THF * 4) M ethanol 5) Water 6) Eluent ) Isopropanol ) M ethanol 3) Water * 4) Isopropanol 5) Eluent To remove non- polar compounds ) Isopropanol ) THF * 3) Dichloromethane 4) Hexane 5) Isopropanol 6) Eluent ) Isopropanol ) M ethanol 3) Dichloromethane 4) Hexane 5) Isopropanol 6) Eluent * Skip this process when you use none chemical bonded silica- gel. * Do not introduce it to fittings that are made out of PEEK!
134 How to wash column W hen there are no problem in separation, peak shape and retention tim e, but still observe an increase in pressure, the cause m ay be from buffer decomposition or filter clogging on the column inlet. Reverse the column and connect it by introducing the following solvents In case of using reverse phase mode (including HILIC mode) Water Isopropanol Water In case of using normal phase mode Isopropanol Caution!! Do not connect the column outlet to the detector! Reduce the flow rate when the pressure is above 0M Pa! Contact each manufacturer before contacting a reversed column washing method. It is difficult to fully regenerate the colum n once it experiences a colum n clogging. The above procedure will tem porary regenerate the colum n only.
135 How to Store column Long term storage differs from overnight or short term storage Colum n ~0 days Few weeks ~ ODS,C8,C4,Ph Eluent without additives 00% M ethanol CN,N H,Diol,SIL Eluent without additives 00% Hexane AX,CX Eluent without additives 00% M ethanol * When the column was not used for a long period of time, please wash the colum n before use.
An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint
An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint An important new column in the Inertsil series Bonded-Phase Structure Perfect balance of retention for both polar
More informationInertSustainSwift C8
Physical Properties Silica Particle Size Surface Area Pore Size Pore Volume Bonded Phase End-capping Carbon Loading ph Range USP Code :ES (Evolved Surface) Silica Gel :.9 μm, μm, μm :00 m /g :00 Å (0 nm)
More informationMethod Development Kits
Method Development Kits sales representative for ordering information or contact...77 Method Development Kits First column choice for Pharma Development USP L C Method Development Kits Maximize retention
More informationPacked Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS. Table of Contents
No. 089 SEPARATION REPORT Packed Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS Table of Contents 1. Introduction 1 2. Column Specification 1 3. Features of Packing Materials
More informationInertsil ODS-EP Technical Information
Technical Information Advantages of The selectivity is completely different from those of conventional columns such as ODS column due to its specific polar group in the stationary phase. Durability can
More informationThe Evolving HPLC Column Packings
The Evolving HPLC Column Packings GL Sciences has been steadily supplying with columns from Inertsil ODS, first-generation to InertSustain series, integration of state-ofthe-art technologies, and has established
More informationInertsil Technical Library
HPLC COLUMN INERTSIL TECHNICAL LIBRARY H P L C C o l u m n Inertsil Technical Library Inertsil ODS-4, C8-4 Comparison of Performance List of Compared Columns Experimental Explanation, Analytical Conditions
More informationInertSustainSwift C8
HPLC, LC/MS Columns InertSustainSwift TM C8 New! InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C8, which
More informationState-of-the-art C18 HPLC Columns
An HPLC GL Sciences Newest and Most Advanced ODS Phase-New For 00 State-of-the-art C HPLC s Improved Peak Shapes and Heights Enhancing Sensitivity High Resolution Fast Equilibration Compatible with 00%
More informationfor Acclaim Mixed-Mode HILIC-1 Column
for Acclaim Mixed-Mode HILIC-1 Column Product Manual for ACCLAIM Mixed-Mode HILIC-1 Page 1 of 17 Product Manual for ACCLAIM Mixed-Mode HILIC-1 Column 5µm, 4.6 x 250mm, P/N 066844 5µm, 4.6 x 150mm, P/N
More informationINERTSIL (GL Sciences)
Inertsil 3 Series The Inertsil 3 series from GL Sciencesʼ represents a major advance in performance over the original Inertsil 2 Series. Inertsil 3 Series phases are based on a purer, higher surface area
More informationAN HPLC COLUMN InertSustain C1 AN HPLC COLUMN InertSustain C1 Inertsil continues to evolve to InertSustain Physical Properties
AN HPLC COLUMN TM InertSustain C8 AN HPLC COLUMN InertSustain TM C8 Inertsil continues to evolve to InertSustain Physical Properties Silica : Newly Developed Silica Gel Particle Size : m, m, m Surface
More informationShodex TM ODP2 HP series columns
HPLC Columns Shodex TM ODP2 HP series columns Better retention of highly polar substances Technical notebook No. 6 Contents 1. Introduction 1-1. Specifications 1-2. Eluent Compatibility of ODP2 HP Series
More informationProduct Bulletin. ACE LC/MS and Rapid Analysis HPLC Columns. HPLC Columns
Product Bulletin HPLC Columns ACE LC/MS and Rapid Analysis HPLC Columns 0 mm, 30 mm, 35 mm and 50 mm column lengths.0,., 3.0, 4.0 and 4.6 mm column diameters Configured for High Sample Throughput Specially
More informationPackings for HPLC. Packings for HPLC
Summary of packings for HPLC In analytical HPLC, packings with particle sizes of 3 to 10 µm are preferred. For preparative separation tasks, also particles with diameters larger than 10 µm are applied.
More informationAdvantages of polymerbased. an alternative for ODS
Advantages of polymerbased HPLC columns an alternative for ODS Yukiko Higai Shodex, 1 Content (1) Comparison between Silica and Polymer-based RP columns (2) Characteristics of Polymer RP columns - an example:
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 1 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationWelcome to our E-Seminar: Choosing HPLC Columns for Faster Analysis Smaller and Faster
Welcome to our E-Sear: Choosing HPLC Columns for Faster Analysis Smaller and Faster High Throughput/Fast LC Requires. Short columns 0 mm or shorter Small particle sizes. µm Rapid Resolution or new.8 µm
More informationBarry E. Boyes, Ph.D. Consumables and Accessories Business Unit May 10, 2000
Barry E. Boyes, Ph.D. Consumables and Accessories Business Unit May 0, 000 HPLC Column Troubleshooting What Every HPLC User Should Know :00 a.m. EST Telephone Number: 86-650-06 Chair Person: Tim Spaeder
More informationSunniest C18 Sunniest RP-AQUA Sunniest C8 Patent pending
Patent pending Website : www.chromtech.net.au E-mail : info@chromtech.net.au telno : 0 97 0... in AUSTRALIA A Novel Bonding Technique (patent pending) An unique trifunctional silyl-reagent was developed
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS penta-hilic Highly retentive, proprietary penta-hydroxy-ligand Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationLC800. Smart HPLC. Until now UHPLC From now Smart HPLC
LC800 Smart HPLC Until now UHPLC From now Smart HPLC Smart HPLC Leads to Ultimate Performance Patent Pending S LC800 is a completely new and unique concept, designed for maximum performance in high resolution
More informationLC and LC/MS Column Selection Flow Chart
LC and LC/MS Column Selection Flow Chart To use the column selection diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the
More information8. Methods in Developing Mobile Phase Condition for C18 Column
I. HPLC Columns Technical Information 8. Methods in Developing Mobile Phase Condition for C18 Column Introduction In reversed phase HPLC, octadecyl group bonded silica columns (C18, ODS) are the most widely
More informationH P L C C O L U M N S
H P L C C O L U M N S New, HxSil TM C8 and C8 HPLC Columns A Word about HPLC Columns For over 0 years Hamilton HPLC columns have been solving problems no other columns could. Now we have introduced HxSil
More informationChemistry Instrumental Analysis Lecture 28. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 28 High Performance Liquid Chromatography () Instrumentation Normal Phase Chromatography Normal Phase - a polar stationary phase with a less polar mobile phase.
More informationThermo Scientific Accucore XL HPLC Columns. Technical Manual
Thermo Scientific Accucore XL HPLC Columns Technical Manual Thermo Scientific Accucore XL HPLC Columns Based on Core Enhanced Technology using µm solid core particles, Accucore XL HPLC columns allow users
More informationInertSustain AQ-C18. HPLC, LC/MS Columns. Maximizing retention for highly polar compounds in reversed phase methods with highly aqueous mobile phases
HPLC, LC/MS Columns InertSustain AQ-C8 Maximizing retention for highly polar compounds in reversed phase methods with highly aqueous mobile phases Physical Properties Silica : ES (Evolved Surface) Silica
More informationHPLC column C18 C8 PFP. SunShell. Core Shell Particle
HPLC column C8 C8 PFP SunShell Core Shell Particle ChromaNik Technologies Inc. SunShell is a core shell silica column made by ChromaNIk Technologies. The next generation to Core Shell particle Features
More informationState - of - the -art C18 HPLC Columns
An HPLC GL Sciences Newest and Most Advanced ODS Phase -New For State - of - the -art C HPLC s Improved Peak Shapes and Heights Enhancing Sensitivity High Resolution Fast Equilibration Compatible with
More informationAlltech Alltima HP Introduction
Alltech Introduction High-Stability, High-Purity, High-Performance, Low-Bleed Columns for Demanding Applications Better Peak Symmetry high-purity silica eliminates peak tailing problems Long Column Life
More informationAcclaim Mixed-Mode WCX-1
Acclaim Mixed-Mode WCX-1 Product Manual for the Acclaim Mixed-Mode WCX-1 Column Page 1 of 28 PRODUCT MANUAL for the Acclaim Mixed-Mode WCX-1 Columns 4.6 x 150 mm, P/N (068353) 4.6 x 250 mm, P/N (068352)
More informationCAPCELL PAK C18 IF2 Type http://hplc.shiseido.co.jp/e/column/html/if2_index.htm Page 1 of 2 List of Sales Representatives Contact Shiseido Technical Materials Catalog List HPLC Columns HPLC Instruments
More informationImpSilHPLC Columns. The solution for your chromatography IMPTECH SCIENTIFIC
ImpSilHPLC Columns The solution for your chromatography IMPTECH SCIENTIFIC 7-1-60,203-Veena Dharam Karam Road,Ameerpet,Hyderabad-500 016 Tel:040-23740443/483 Ph:9848051071 imptechscientific@gmail.com www.impscience.com
More informationToo Polar for Reversed Phase What Do You Do?
Too Polar for Reversed Phase What Do You Do? June 20, 2013 Mark Powell Columns and Consumables Technical Support Page 1 C8 or C18 Doesn t Always Do the Job Typical reversed phase conditions involve water/buffer
More informationRP C18 column with feature of a silanol group
RP C8 column with feature of a silanol group lanol Activity Controlled C8 Column Sunrise ctacosyl C8) Sunrise ctadecyl C8) Sunrise ctadecyl-sac C8-SAC has an interaction of silanol groups ChromaNik Sunrise
More informationHypersil BDS Columns TG 01-05
TG 0-0 Hypersil BDS Columns Introduction Hypersil BDS columns have gained a reputation over the years as one of the most robust, reproducible and reliable HPLC column brands available. This Technical Guide
More informationAgilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX
Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX Technical Note Agilent s SampliQ WAX provides Applications for strongly acidic, acidic and neutral compounds Excellent
More informationProntoSIL C18-EPS Reversed-Phase HPLC Columns
ProntoSIL C8-EPS Reversed-Phase HPLC Columns Provides excellent separation of polar compounds Better peak shape for acids and bases Stabilized bonded phase for rugged, robust HPLC methods More retentive
More informationC18 Column. Care & Use Sheet
C18 Column Care & Use Sheet HALO Description HALO C18 is a high-speed, high-performance liquid chromatography column based on a new Fused-CoreTM particle design. The Fused-Core particle provides a thin
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS Highly retentive, proprietary penta-hydroxy-ligand penta-hilic Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationFast Separation of Vastly Different Compounds by Isocratic HPLC
Fast Separation of Vastly Different Compounds by Isocratic HPLC Aimee N. Heyrman and Yury Zelechonok Resolution Systems, Inc. 590 E. 32nd St. Holland. MI, 49423 SIELC Technologies, 15 E. Palatine Rd, Suite
More informationOptimisil HPLC & UPLC Column catalogue
Optimisil HPLC & UPLC Column catalogue Analytical Hub Plot No: 37, P & T Colony, Vikrampuri, Secunderabad 500009 Tel: 040-64621313, Mobile: +91 7799931313 Website: www.analyticalhub.com email: contact@analyticalhub.com
More informationACE Ultra Inert Base Deactivated HPLC Columns
ACE Ultra Inert Base Deactivated HPLC Columns Contents Page ACE HPLC Columns...- Independent Comparison of HPLC Columns #... Independent Comparison of HPLC Columns #... ACE 00Å HPLC Columns Specifications...-
More informationComparison of different aqueous mobile phase HPLC techniques
June 009 ewsletter Pharmaceutical Analysis: What is Your Problem? SIELC Technologies, Inc., Prospect Heights, IL 0070 Pharmaceutical analysis involves liquid chromatography of various compounds, from active
More informationLC Column Troubleshooting. Do you have an equivalent column for my LC column?
LC Column Troubleshooting Do you have an equivalent column for my LC column? LC columns from different suppliers may have very different retention properties even if the bonding is the same. Although there
More informationCapital HPLC Columns and HPLC Cartridges. Optimal HPLC Range
Capital HPLC Columns and HPLC Cartridges Specialty Columns HPLC Columns & HPLC Cartridges Download Capital Catalog Adsorbosphere Apex Chromegabond Diasogel Exsil Hypersil Inertsil ODS-2 Partisil Rosil
More informationBasic principles of HPLC
Introduction to the theory of HPLC HPLC (High Performance Liquid Chromatography) depends on interaction of sample analytes with the stationary phase (packing) and the mobile phase to effect a separation.
More informationThe Secrets of Rapid HPLC Method Development. Choosing Columns for Rapid Method Development and Short Analysis Times
The Secrets of Rapid HPLC Method Development Choosing Columns for Rapid Method Development and Short Analysis Times Rapid Analysis Is More Than Run Time It is developing a method to meet a goal and developing
More informationfor Acclaim Mixed-Mode WCX-1
for Acclaim Mixed-Mode WCX- Product Manual for the Acclaim Mixed-Mode WCX- Column Page of 8 Product Manual for Acclaim Mixed-Mode WCX- Columns Acclaim Mixed-Mode WCX-, 3µm, Analytical column, 3.0 x 50mm
More informationACE Excel SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE Excel SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS-compatible buffers between ph.. High efficiency µm, µm,
More informationPacked columns for reversed-phase chromatography TSKgel ODS-100V and 100Z
Packed columns for reversed-phase chromatography TSKgel ODS-100V and 100Z Contents Page 1. Introduction 2 2. Specifications and characteristics 2 3. Properties of packing material 3 4. Chromatographic
More informationP R O D U C T B U L L E T I N. Fused-Core particle technology for hyper-fast and super-rugged HPLC columns
P R O D U C T B U L L E T I N Fused-Core particle technology for hyper-fast and super-rugged HPLC columns HALO column packings are not made the typical way. Instead, the particles packed into HALO columns
More informationTechnical Guide Thermo Scientific Syncronis HPLC Columns
Technical Guide Thermo Scientific Syncronis HPLC Columns Consistent, predictable separations, Column after column, time after time Thermo Scientific Syncronis Columns When developing a new method, one
More informationLC Technical Information
LC Technical Information Method Transfer to Accucore.6 μm Columns Containing solid core particles, which are engineered to a diameter of.6μm and a very narrow particle size distribution; Accucore HPLC
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm, µm and
More informationSample Preparation Products General Catalogue. Sample Preparation Products General Catalogue
Sample Preparation Products General Catalogue Sample Preparation Products General Catalogue Solid Phase Cross Reference 3 Objective of Solid Phase Extraction 4 5 Usage of Solid Phase Extraction 6 7 SPE
More information637. Thiamethoxam. HPLC method
637. Thiamethoxam HPLC method CIPAC Collaborative Trial according to CIPAC Information Sheet N o 293 Dr. Sven Adolph Syngenta Crop Protection Münchwilen AG CH-4333 Münchwilen Switzerland May 212 page 1
More informationANALYTICAL METHOD DETERMINATION OF VOLATILE ALDEHYDES IN AMBIENT AIR Page 1 of 11 Air sampling and analysis
DETERMINATION OF VOLATILE ALDEHYDES IN AMBIENT AIR Page 1 of 11 Replaces: Dated: Author: Date: AM-No.: New New Nils Arne Jentoft 18.06.2014 0 CHANGES This procedure is new. 1 SCOPE This document describes
More informationNew Product Update. HPLC Columns
New Product Update HPLC Columns ZORBAX MicroBore HPLC Columns ZORBAX Extend-C18 Columns ZORBAX Bonus-RP Columns Inertsil ODS-2 Cartridge Columns ZORBAX Carbohydrate Analysis Columns ZORBAX Eclipse dsdna
More informationACQUITY UPLC HSS Columns
CONTENTS I. INTRODUCTION II. II. GETTING STARTED a. Column Connectors b. Column Installation c. Column Equilibration d. ecord Installation e. Initial Column Efficiency Determination f. VanGuard Pre-columns
More information(HILIC) Bill Champion Agilent Technologies, Inc opt 3/opt3/opt 2 HILIC - Agilent Restricted
Hydrophilic Interaction Chromatography (HILIC) Bill Champion Agilent Technologies, Inc. 800-227-9770 opt 3/opt3/opt 2 william_champion@agilent.com Page 1 HILIC A method of recent attention Bill Champion
More informationAcclaim Mixed-Mode WAX-1 Columns
User Manual Acclaim Mixed-Mode WAX- Columns 06565 Revision 03 October 05 Product Manual for the Acclaim Mixed-Mode WAX- Column Page of 3 Product Manual for Acclaim Mixed-Mode WAX- Columns 5µm, 0 x 50 mm,
More informationHPLC Column. Technical Guide
HPLC Column Technical Guide (1) Introduction P 1 (2) Specifications P 1 ~ 4 (3) Shipping Solvent P 5 ~ 8 (4) Mobile Phase P 9 ~ 11 (5) Sample Diluent/Injection Solvent P 12 (6) Column Cleaning and Storage
More informationH 3 CO H 3 CO S CH 3
FENITROTHION 35 H 3 CO P H 3 CO S O CH 3 NO 2 ISO common name Chemical name Fenitrothion O,O-Dimethyl O-4-nitro-m-tolyl phosphorothioate (IUPAC) O,O-Dimethyl O-(3-methyl-4-nitrophenyl)- phosphorothioate
More informationODS-UG,HG,MG,SR columns
Column for reversed phase ODS-UG,HG,MG,SR columns Develosil ODS-HG Develosil ODS-UG Develosil ODS-MG Develosil ODS-SR The 2nd generation of Develosil series, very standard column This series is the column
More informationOverview Applications of NUCLEODUR HPLC Phases
verview Applications of HPLC Phases Phase Specification Characteristics* Stability Structure A B C octadecyl phase, high density coating multi-endcapping 8% C USP L ph stability -, suitable for LC/MS (Si-
More informationHigh Resolution Fast LC
High Resolution Fast LC Easier Than You Think Rita Steed LC Columns Application Engineer May 9, 2013 What is High Resolution Fast LC? Maintain Resolution with Faster Run Time Increased Resolution with
More informationHigh Performance Liquid Chromatography
STANDARDBASE techniques: High Performance Liquid Chromatography Drenthe College, The Netherlands 1. Introduction HPLC. High Performance Liquid Chromatography High Performance Liquid Chromatography (HPLC)
More informationLIQUID CHROMATOGRAPHY
LIQUID CHROMATOGRAPHY RECENT TECHNIQUES HPLC High Performance Liquid Chromatography RRLC Rapid Resolution Liquid Chromatography UPLC Ultra Performance Liquid Chromatography UHPLC Ultra High Pressure Liquid
More informationKromasil. for your analytical HPLC. Kromasil. The way to peak performance in liquid chromatography
Kromasil for your analytical HPLC Kromasil The way to peak performance in liquid chromatography Kromasil is known, worldwide, for its high performance and excellent total economy in preparative and industrial
More informationImprove Peak Shape and Productivity in HPLC Analysis of Pharmaceutical Compounds with Eclipse Plus C8 Columns Application
Improve Peak Shape and Productivity in HPLC Analysis of Pharmaceutical Compounds with Eclipse Plus C8 Columns Application Pharmaceuticals Authors John W. Henderson Jr., Nona Martone, and Cliff Woodward
More informationTracer Excel TRACER EXCEL ODS-A. Total deactivation. Maximum Stability
Tracer Excel TRACER EXCEL is a range of totally new packings that employ the most advanced procedures of synthesis and chemical functionalization, resulting in some column packings that completely surpass
More informationLUMEFANTRINUM LUMEFANTRINE
July 2008 LUMEFANTRINE: Final text for addition to The International Pharmacopoeia (July 2008) This monograph was adopted at the Forty-second WHO Expert Committee on Specifications for Pharmaceutical Preparations
More informationCOLUMN GUIDE - Usage and maintenance of HPLC columns
COLUMN GUIDE - Usage and maintenance of HPLC columns 1. HPLC 1.1 Introduction The HPLC columns manufactured by VDS optilab Chromatographietechnik GmbH are, on the basis of modern technology, filled with
More informationHPLC Column for Structual Isomers
PLC Column for Structual Isomers lica Gel Average Particle ze Average Pore ze Stationary Phase Main Interaction Carbon Content CSMSIL PYE CSMSIL PE igh Purity Porous Spherical lica 2-(1-Pyrenyl)ethyl Group
More informationAcclaim HILIC-10 Columns
Thermo Scientific Acclaim HILIC- Columns Product Manual P/N: January 3 Part of Thermo Fisher Scientific Product Manual for Acclaim HILIC- Analytical Column Acclaim HILIC-, 3 µm, Analytical, 4.6 x 5 mm
More informationReversed Phase Solvents
Part 1. General Chromatographic Theory Part 2. verview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use Reversed Phase Solvents 2 Solvents for RP Chromatography
More informationSTUDY OF LOADABILITY AND SELECTIVITY OF PHARMACEUTICAL COMPOUNDS ON RPLC COLUMNS
STUDY OF LOADABILITY AND SELECTIVITY OF PHARMACEUTICAL COMPOUNDS ON RPLC COLUMNS Fang Xia, Jie Y. Cavanaugh, Uwe Neue, Jeffrey R. Mazzeo, Diane M. Diehl Waters Corporation, Milford, MA USA OVERVIEW- INTRODUCTION-
More information1.7 m Fortis UHPLC Columns
Strength in Technology 1.7 m Fortis Columns Ultra High Pressure Chromatography Improve your Performance 8 Chemistry Choices Increase Efficiency Increase Speed Improve Resolution Greater Sensitivity Lower
More informationOpen Column Chromatography, GC, TLC, and HPLC
Open Column Chromatography, GC, TLC, and HPLC Murphy, B. (2017). Introduction to Chromatography: Lecture 1. Lecture presented at PHAR 423 Lecture in UIC College of Pharmacy, Chicago. USES OF CHROMATOGRAPHY
More informationDilution(*) Chromatography
WA20264 Poster # 184, HPLC 2002, Montreal, 4-5 June 2002 At-Column Column-Dilution for Preparative Dilution(*) Chromatography Cecilia Mazza, Jie Cavanaugh, Ziling Lu,Tom Sirard,Tom Wheat and Uwe Neue Waters
More informationMethod Development in Solid Phase Extraction using Non-Polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples
Technical Note 101 Method Development in Solid Phase Extraction using Non-Polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples This technical note includes by specific information on the extraction
More informationTechnical Information
216 Miscellaneous Miscellaneous 217 Technical Information Contents Column Handling... 218-220 Mobile Phases for RP-Columns... 221 HPLC Column Performance... 222 Inspection Reports... 223 FAQ... 224-227
More informationChromatography. Gas Chromatography
Chromatography Chromatography is essentially the separation of a mixture into its component parts for qualitative and quantitative analysis. The basis of separation is the partitioning of the analyte mixture
More information[ CARE AND USE MANUAL ]
CONTENTS I. INTRODUCTION II. III. IV. GETTING STARTED a. Column Installation b. Column Equilibration c. Initial Column Efficiency Determination COLUMN USE a. Guard Columns b. Sample Preparation c. ph Range
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm,
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationHPLC Column Material - Bulk Ware
HPLC Column Material - Bulk Ware Constantly growing demands on separation efficiency and availability of solid phase material for packing HPLC columns and column packing stations have motivated us to provide
More informationGuide to Choosing and Using Polymer Reversed Phase Columns!
Guide to Choosing and Using Polymer Reversed Phase Columns! Choosing the best RP Column to use: Silica is normally used as the packing material for HPLC columns for a number of reasons. It is very strong,
More informationMEDROXYPROGESTERONE INJECTION
September 2011 RESTRICTED DRAFT PROPOSAL FOR The International Pharmacopoeia MEDROXYPROGESTERONE INJECTION (September 2011) DRAFT FOR COMMENT Should you have any comments on the attached draft proposal,
More informationTECHNICAL BRODIFACOUM
BRODIFACOUM Full specification: Approved 10 December 1999 1. Specification 1.1 Description The material shall consist of brodifacoum together with related manufacturing impurities and shall be in the form
More informationIon Exchange Columns ---- Inertsil AX 078 Inertsil CX 080. HPLC Column Catalog
Ion Exchange ---- Inertsil AX 078 Inertsil CX 080 HPLC Column Catalog 077 Inertsil AX Physical Properties Silica : Series High Purity Silica Gel : μm Surface Area : 0 m /g Pore Size : 00 Å (0 nm) Pore
More informationHPLC UHPLC Columns. Dikma Technologies Inc.
Technologies Inc. About HPLC UHPLC Columns has over years of R&D experience in HPLC / UHPLC columns. HPLC / UHPLC columns have been widely used by pharmaceutical manufacturers. We offer columns of ultimate
More informationDetermination of 24 PAHs in Drinking Water
Determination of 24 PAHs in Drinking Water Application Note Food Testing and Agriculture Authors M.C. Díaz Ramos, A. Suárez, A. Rúbies, and R. Companyó Laboratori de l Agencia de Salut Publica de Barcelona
More informationTransferring Yesterdays Methods to Tomorrows Technology: The Evolution from HPLC to UPLC TM
Transferring Yesterdays Methods to Tomorrows Technology: The Evolution from HPLC to UPLC TM Eric S. Grumbach Thomas E. Wheat Chuck Phoebe Joe Arsenault Jeffrey R. Mazzeo Diane M. Diehl UPLC TECHNOLOGY
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Inertsil. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Inertsil Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationADVANTAME (TENTATIVE)
ADVANTAME (TENTATIVE) SYNONYMS INS No. 969 New tentative specifications prepared at the 77th JECFA (2013) and published in FAO JECFA Monographs 14 (2013). An ADI of 0-5 mg/kg body weight was established
More informationOrosil HPLC Columns. OroSil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium ph.
Orosil OroSil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium ph. Excellent organic base selectivity with very low asymmetry values Compatible
More information