Original Paper. i 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 1 Introduction. Andrea Hercegovµ. Eva Matisovµ
|
|
- Martha Townsend
- 5 years ago
- Views:
Transcription
1 1102 A. Hercegovµ et al. J. Sep. Sci. 2006, 29, Andrea Hercegovµ Milena Dömötörovµ DµÐa Kružlicovµ Eva Matisovµ Institute of Analytical Chemistry, Slovak University of Technology, Faculty of Chemical and Food Technology, RadlinskØho, Bratislava, Slovak Republic Original Paper Comparison of sample preparation methods combined with fast gas chromatography mass spectrometry for ultratrace analysis of pesticide residues in baby food Four sample preparation techniques were compared for the ultratrace analysis of pesticide residues in baby food: ( modified Schenck s method based on ACN extraction with SPE cleaning; (b) quick, easy, cheap, effective, rugged, and safe (QuEChERS) method based on ACN extraction and dispersive SPE; (c) modified QuEChERS method which utilizes column-based SPE instead of dispersive SPE; and (d) matrix solid phase dispersion (MSPD). The methods were combined with fast gas chromatographic-mass spectrometric analysis. The effectiveness of clean-up of the final extract was determined by comparison of the chromatograms obtained. Time consumption, laboriousness, demands on glassware and working place, and consumption of chemicals, especially solvents, increase in the following order QuEChERS a modified QuEChERS a MSPD a modified Schenck s method. All methods offer satisfactory analytical characteristics at the concentration levels of 5, 10, and 100 lg/kg in terms of recoveries and repeatability. Recoveries obtained for the modified QuE- ChERS method were lower than for the original QuEChERS. In general the best LOQs were obtained for the modified Schenck s method. Modified QuEChERS method provides 21 72% better LOQs than the original method. Keywords: Baby food / Fast GC-MS / Pesticide residues / Quadrupole mass selective detector / Sample preparation / Received: November 3, 2005; revised: January 10, 2006; accepted: January 31, 2006 DOI /jssc Introduction According to the status list of all active substances on the EU market (Status of active substances under EU review (doc. 3010), more than 1100 pesticides substances or products that kill harmful organisms (pests) are currently registered. There are significant economic benefits associated with the use of pesticides. Pesticides are used by farmers to improve or safeguard yields, to improve or protect quality of the produce, and to minimize labor input. Unfortu- Correspondence: Professor Eva Matisovµ, Institute of Analytical Chemistry, Slovak University of Technology, Faculty of Chemical and Food Technology, RadlinskØho 9, SK Bratislava, Slovak Republic. eva.matisova@stuba.sk. Fax: Abbreviations: MSPD, matrix solid phase dispersion; PSA, primary secondary amine; PTV, programmed temperature vaporizer; QuEChERS, quick, easy, cheap, effective, rugged, and safe; R, recovery nately, these substances are often also harmful to nontarget organisms. Consumers can be subject to indirect exposure, due to the presence of pesticide residues in agricultural produce. Possible long-term health effects of pesticide residue exposure may include cancer, acute and chronic injury to the nervous system, neuromuscular and mental deficits, reproductive problems, and many others. Particular attention must be devoted to an especially sensitive population group children. The European Commission specified the maximum residual limit (MRL) of 10 lg/kg of pesticide residue content in cereal-based foods and baby foods for infants and young children [1]. In our recent publication [2] the modified Schenck s method connected with fast GC was used to analyze pesticide residues in baby food utilizing common routine instrumentation (with quadrupole mass spectrometric (MS) detection). Also matrix solid-phase dispersion (MSPD) was successfully combined with fast GC-MS on commercially available instrumentation analyzing
2 J. Sep. Sci. 2006, 29, Sample preparation methods fast GC-MS for pesticide residues analysis 1103 apples (the most common raw material for baby food production) [3]. Nowadays the research in the field of sample preparation methods of plant matrix heads toward faster, less laborious, less harmful, and cost-effective procedures, preserving high recoveries and good precision, and are multiresidual and rugged. In 2003, Anastassiades et al. [4] introduced the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method for the analysis of pesticide residues in fruit and vegetables. In a follow-up study, Lehotay et al. [5] conducted validation experiments of the QuEChERS method for more than 200 pesticides in several matrixes using GC-MS and LC-MS-MS. The results were satisfactory for nearly all pesticide residues in lettuce and orange. Leandro et al. [6] applied the QuEChERS method to the determination of 12 priority pesticides in baby food at a concentration level below 10 lg/kg using GC-MS-MS instrumentation. Connection of advantages of both modern approaches in sample preparation and fast GC techniques promises unquestionable benefits [7], such as high throughput, low GC operating cost, simplicity, higher sensitivity, and more reliable analytical answer. Our recent studies proved that the connection of fast GC utilizing microbore column (with id of 0.15 mm) with a common bench top quadrupole mass spectrometer provides efficient separation of 18 studied pesticides from the apple matrix components [2], sufficient number of data-points acquired for the proper peak shape reconstruction and good repeatability of peak areas in SIM mode, and the good overall system ruggedness after repeated matrix injections [8, 9]. The purpose of this study is to evaluate different sample preparation methods combined with fast GC-MS with a quadrupole bench top mass selective detector (MSD) for the determination of pesticide residues in baby food. Four sample preparation methods were compared: ( modified Schenck s method based on ACN extraction, followed by liquid liquid partitioning (LLP) of the crude extract and clean-up with SPE; (b) QuEChERS method based on ACN extraction and dispersive SPE; (c) modified QuEChERS method which utilizes SPE on columns instead of dispersive SPE; and (d) MSPD method, which involves cell disruption, homogenization, extraction into ethyl acetate, and a clean-up step in one step. Results of validation at the concentration levels of 5, 10, and 100 lg/kg; reached LOQs; as well as laboriousness, speed, and cleaning effectiveness are taken as criteria for method evaluation. The used chromatographic method in this paper was optimized with respect to the speed/ separation efficiency trade-off [10]. Analytical columns of inner diameter of 0.15 mm id were utilized due to a compromise between the time of analysis, transport phenomena, and column capacity concerning complex analytical samples. The 0.15 mm id columns allow more flexibility in loadability, sample introduction (flow rate), and operation when compared to 0.10 mm id columns [11]. Moreover, the ruggedness of 0.15 mm id columns was shown to be very good in the analysis of pesticide residues in plant matrix [9]. Several ways to reduction of matrix effects on the injector and column site were practiced [2, 9, 12, 13] (Document N SANCO/10476/2003, REV.4, food/plant/protection/evaluation/guidance/wrkdoc12_ en.pdf). 2 Experimental 2.1 Reagents and materials Pesticide reference standards of purity A 95% were obtained from various sources (Table 1). Stock solution of 0.5 mg/ml was prepared in toluene (Suprasolv, Merck, Darmstadt, Germany) and stored at 188C. Working standard pesticide mixtures prepared in acetone (Suprasolv, Merck) were used for preparation of spiked samples and matrix-matched standards. Standards were weighed on Sartorius Analytic MCI balances (Sartorius, Göttingen, Germany) with a precision of l10 lg. ACN and ethyl acetate used were of GC grade (Suprasolv, Merck). Magnesium sulfate (anhydrous powder) and NaCl p.a. was from Lachema (Neratovice, Czech Republic). The sorbent Florisil ( mesh) was from Rotichrom, Roth, Karlsruhe, Germany. Bulk primary secondary amine (PSA) sorbent was obtained from Varian (Varian Incorporated, Harbor City, USA), the SPE columns used were 500 mg Bond-Elut- NH 2 (IST, Mid Glamorgan, UK). Quartz glass wool was Table 1. List of pesticides, chemical classes, and monitored ions utilizing GC-MS in SIM mode Pesticide Chemical class Retention time (min) Monitored ions in SIM mode, Target ion Dimethoate Organophosphorus , 125 Terbuthylazine Triazine , 229 Diazinon Organophosphorus , 304 Pyrimethanil Anilinopyrimidine , 199 Chlorpyrifos-methyl Organophosphorus , 288 Fenitrothion Organophosphorus , 277 Chlorpyrifos Organophosphorus , 314 Cyprodinil Anilinopyrimidine , 225 Penconazole Triazole , 250 Methidathion Organophosphorus , 302 Kresoxim-methyl Oximinoacetate , 132 Myclobutanil Triazole , 245 Tebuconazole Triazole , 252 Phosalone Organophosphorus , 367 Bitertanol 1 Triazole , 170 Bitertanol Cypermethrin 1 Pyrethroid , 181 Cypermethrin Cypermethrin Etofenprox Nonester pyrethroid , 376 Pesticides are arranged according to retention times. Target ion used for quantification.
3 1104 A. Hercegovµ et al. J. Sep. Sci. 2006, 29, from Agilent Technologies (Avondale, PA, USA). For filtration purposes glass fiber paper (Papírna PerÐtejn, Czech Republic) was used. For preconcentration purposes, nitrogen of purity A 99.99% (Linde, Technoplyn, Bratislava, Slovak Republic) was used. 2.2 Sample preparation The apples with peel were homogenously mixed with blender Braun MX 2050 (Kronberg, Germany). The apples used for validation purposes were checked by GC-MS for the studied pesticide residues and none of the selected ions were found at the corresponding retention times of the selected pesticides Modified Schenck s method Twenty-five grams of apple sample was extracted with 50 ml of ACN using sonication sonda of the pulsed ultrasonic cell disrupter VibraCell (Sonics and Materials, Danbury, CT, USA, CVX 400, frequency 20 khz). The ultrasonic pulses at 80% amplitude with duration of 3 s paused for 3 s were applied for 1 5 min. The extract was filtered through glass fiber paper (Papírna PerÐtejn), and the filtrate was transferred into an Erlenmayer flask with a tap. NaCl (2.5 g) was added and the mixture was shaken for 1 min. Phases were allowed to separate for 15 min. The upper ACN phase was transferred into an Erlenmayer flask, anhydrous magnesium sulfate (2 g) was added, and the mixture was shaken for 1 min. Twenty-five microliters of the dried extract was evaporated to less than 1 ml in a vacuum evaporator and transferred into an SPE-NH 2 column. Magnesium sulfate (1 cm layer) was always added to the top of the SPE column; the column was previously conditioned with acetone. The eluates were collected into 20 ml vials. Analytes were eluted with 15 ml of acetone and eluates were evaporated to dryness under a stream of nitrogen. The final volumes of the extracts were adjusted with toluene to 5 ml and analyzed by GC- MS. Preconcentration factor of the method is MSPD Five grams of a sample and 8 g of Florisil were blended in a glass mortar, transferred into a 25 cm615 mm id glass column plugged with glass wool and containing a layer of 2.5 g anhydrous magnesium sulfate. The column head was covered with a second 2 mm layer of anhydrous magnesium sulfate. The column was eluted with 60 ml of ethyl acetate by gravitational flow. The eluate was collected, concentrated to dryness using a rotary vacuum evaporator, then the dry rest was dissolved in 1 ml of toluene (preconcentration factor 5) QuEChERS Ten grams of apple sample weighed into the 40 ml centrifuge tube was extracted with 10 ml of ACN using Ultra-Turrax (IKA, Germany) homogenizer at rpm for 3 min. Then LLP followed: 1 g NaCl and 4 g MgSO 4 were added and the mixture was shaken by hand for 1 min. The mixture was then centrifuged at 3000 rpm for 5 min. Portion of the upper layer was transferred into a 10 ml centrifuge tube containing 25 mg PSA sorbent and 125 mg MgSO 4 per 1 ml of the cleaned extract. The mixture was shaken by hand for 1 min, and then centrifuged for 5 min at 3000 rpm to separate solids from solution. Extract of minimum 1 ml (Document N SANCO/ 10476/2003, REV.4, plant/protection/evaluation/guidance/wrkdoc12_en.pdf) was transferred into the vial and evaporated under N 2 to dryness to perform solvent exchange to toluene at preconcentration factor Modified QuEChERS After the first centrifugation of the original QuEChERS method, 5 ml of the upper layer was transferred onto an SPE column filled with acetone-conditioned 0.5 g of NH 2 sorbent covered with 1 cm layer of MgSO 4. The SPE column was eluted with 10 ml of acetone. The cleaned extract was evaporated under N 2 to dryness, and the solvent exchange to 2 ml of toluene was performed (preconcentration factor 2.5). 2.3 GC-MS GC MS measurements were performed on an Agilent 6890N GC coupled to 5973 MSD (Agilent Technologies, Avondale) equipped with a programmed temperature vaporizer (PTV) and autoinjector Agilent MS with electron impact ionization (EI) mode (70 ev) was operated in SIM mode; for each pesticide two specific ions were selected and sorted into groups (maximal number of ions in one group was eight); the used dwell time was 10 ms. PTV was operated in cold splitless mode. The injection volume was 2 ll. Helium with purity 5.0 (Linde Technoplyn) was used as a carrier gas. Microbore chromatographic column CP-Sil 8 CB (Varian, Middelburg, The Netherlands) with 5% diphenyl 95% dimethylsiloxane stationary phase 15 m60.15 mm id60.15 lm was utilized. It was connected to a nonpolar deactivated precolumn (1 m60.32 mm id) for focusation purposes and better ruggedness of the chromatographic system [9, 13]. Constant pressure mode kpa was used until the elution of the last analyte (etofenprox, 7.90 min); additional pressure ramp (1000 kpa/min, 685 kp was used to speed up elution of higher boiling matrix coextractives. Chromatographic separation was performed under a temperature program, 1308C (1.13 min), C/min, 2908C (8 min). PTV conditions: temperature program, 1508C,
4 J. Sep. Sci. 2006, 29, Sample preparation methods fast GC-MS for pesticide residues analysis C/min, 3008C (2 min), 4008C/min, 3508C (5 min); split vent open time 1.13 min. (The modified Schenck s method was validated at slightly different temperature and pressure conditions [2]: concerning oven temperature programme 1208C, 308C/min, 2908C (5 min), and constant carrier gas flow 0.5 ml/min.) 2.4 Real samples Apples were obtained from the suppliers of raw material to baby food production. Apples of cultivar Topaz were obtained from locality NovØ Zµmky. Apples of cultivar Jonagold were of origin from Dvory nad Žitavou and were used in the technological process of baby food production-apple pureø (Novofruct s.r.o., NovØ Zµmky, Slovak Republic). Sampling apple pureø after each step of the technological process was carried out by the following steps: (i) warming up to 608C for ca. 15 min; (ii) heating to 808C for 30 min; (iii) heating the mixture to 908C and (iv) sterilization at 988C for 40 min. 3 Results and discussion The studied pesticides were chosen according to the statistical evaluation of the consumption of pesticides applied to the apple trees in the south-west part of Slovakia. Farmers from this region have delivered the raw material to the producer of baby food. Pesticides also represent a wide range of polarity and other physicochemical properties. 3.1 Development of sample preparation methods Method development concerning the modified Schenck s method and MSPD are published in our recent publications [2, 3]. In the original QuEChERS procedure certain changes were made according to our needs and possibilities: Comminution with a chopping device with dry ice was replaced by mixing in a blender, and therefore the homogenization with Ultra Turrax was used at the extraction step instead of shaking to ensure good extraction efficiencies. Various extraction conditions were tested [14]. Five microliters of the extract after the first centrifugation was taken instead of 1 ml for the reason that more final extract was obtained. Solvent exchange (2 ml of ACN extract to 2 ml of final toluene extract) was included to permit better comparison of results and for the convenience to use the same GC method developed for the solvent toluene. Also the effectiveness of different amounts (25 75 mg) of PSA sorbent was assessed with regard to the quantity of coextractives remaining in the final extract. This was determined by the comparison of chromatograms of apple matrix obtained in the full scan mode. Twenty-five milligrams PSA sorbent/1 ml extract was found sufficient [14]. The main disadvantage of the original QuEChERS method compared to the other common methods is that the 1 g/ml final extract concentration is lower than the 2 5 g/ml concentrated extracts of the most traditional methods. If matrix is not the limiting source of noise in the analysis, this leads to higher LOQs for the same injection volume in the QuEChERS method. We investigated also the option of concentrating the final extracts to yield 2.5 g/ml (equally to modified Schenck s method). In order to reach cleaner extracts in the modified QuE- ChERS method, the dispersive SPE was replaced by cartridge-based SPE using NH 2 sorbent. Some interfering peaks were removed when moving from dispersive to column-based SPE Cleaning efficiency comparison A critical aspect of pesticide residue analysis is the purification, which is required to isolate the residues from matrix components to reduce undesirable matrix effects and is essential for the sufficient column capacity and the satisfactory long-term chromatographic performance during the analysis of a range of samples. The effectiveness of clean-up of the final extract (expressed by those coextractants which elute from the column under the given chromatographic conditions) was compared for all sample preparation methods. Similar chromatograms of unspiked apple samples in the full scan mode were obtained for the modified Schenck s and the modified QuEChERS method (both with concentration 2.5 g/ ml of the final extract). Two times concentrated MSPD final extract (5 g/ml) shows different coextractants, which, with their intensity, represent the worst burden on the chromatographic system. Figure 1 shows extracted ion chromatograms of selected pesticides in apple matrix in SIM mode. In all cases no significant interfering peaks were observed at the higher ion masses m/z. For lower, nonspecific m/z ions (pesticides methidathion m/z 145, kresoxim-methyl 131, and etofenprox 163) more impurities were detected in the areas of interest; however, the 15 m microbore column with 0.15 mm id provides satisfactory resolution in the case of original and modified QuEChERS and MSPD. Modified Schenck s method provides satisfactory cleaning also from interferences giving lower m/z ions. These observations relate to LOQs (Section 3.2.2) Comparison on laboriousness and speed The differences among methods as well as the demands on chemicals, material, and the time were considered. The most time consuming step of the single sample preparation procedure is evaporation under N 2. If there is a possibility to evaporate more extracts at the same time (by simple arrangement of the device or using of a Turbovap), the time limiting factor of the preparation of more
5 1106 A. Hercegovµ et al. J. Sep. Sci. 2006, 29, Figure 1. Extracted chromatograms of target ions of pesticides in the apple matrix-matched standard solution from fast GC-MS measurements in SIM mode. (A) Modified Schenck's method; (B) QuEChERS method; (C) modified QuEChERS method; (D) MSPD, concentration of pesticides 5 lg/kg. (1) Dimethoate; (2) terbuthylazine; (3) diazinon; (4) pyrimethanil; (5) chlorpyrifosmethyl; (6) fenitrothion; (7) chlorpyrifos; (8) cyprodinil; (9) penconazole; (10) methidathion; (11) kresoxim-methyl; (12) myclobutanil; (13) tebuconazole; (14) phosalone; (15) bitertanol; (16) cypermethrin; and (17) etofenprox.
6 J. Sep. Sci. 2006, 29, Sample preparation methods fast GC-MS for pesticide residues analysis 1107 Figure 2. Results of the R experiments of pesticide residues from apples at spiking levels of 5, 10, and 100 lg/kg. samples becomes evaporation in a rotary evaporator. From this point of view the most time-consuming methods are the modified Schenck s method and MSPD. As in the modified Schenck s method it is necessary to carry out evaporation under N2 and the cleaning step on SPE columns, this method is unambiguously the most time consuming and the most laboriousness approach. The original QuEChERS method does not involve the solvent exchange step, and therefore it is the fastest and less laboriousness method. In terms of time, space, and financial demands, laboriousness, material, and solvents consumption we offer the following order: QuEChERS a modified QuEChERS a MSPD a Schenck s method. i 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim 3.2 Validation results Experience tells us that if the method works well for the selected representative pesticides, then it should work equally well for nearly all of the others monitored routinely in multiclass, multiresidue methods [15] Recovery (R) studies R studies were carried out by spiking untreated apple samples with known volumes of the appropriate working mixtures of pesticides. The mixture was left to stand for 30 min. Blank samples were spiked with the pesticides at three concentration levels 5, 10, and 100 lg/kg.
7 1108 A. Hercegovµ et al. J. Sep. Sci. 2006, 29, Except dimethoate in the case of QuEChERS and modified QuEChERS method and phosalone in the case of modified Schenck s method all pesticides analyzed by each sample preparation method tested met commonly accepted validation requirements of % recoveries [16] (Fig. 2). Vast majority of R results exceed 80%. Differences due to analyte concentration were minimal in terms of R for all methods and nearly all analytes. Recoveries obtained for the modified QuEChERS method were lower than those for the original QuEChERS for all pesticides except for pesticide cypermethrin at the level of 5 lg/kg. In terms of repeatability (expressed as RSD) the vast majority of pesticides gave a 10% RSD. Except four cases (three for MSPD and one for modified Schenck s method) all met the criterion of f20% RSD [16]. RSD increased to some extent as concentration decreased. Table 3. Pesticide etofenprox content in apple samples obtained from a supplier to baby food production analyzed by modified Schenck s, QuEhERS and modified QuEChERS sample preparation methods connected with fast GC-MS Method Average concentration (lg/kg) RSD PA (%) RSD GC (%) Modified Schenck s QuEChERS Modified QuEChERS RSD PA RSD of analysis of two parallel sample extractions (two test portions), calculated by Eckschlager et al. [17]. RSD GC RSD of GC-MS measurements (the highest value is given), n = 2, calculated by Eckschlager et al. [17]. In all methods compared Ultra turrax was used in the first extraction step; 10 m mm60.1 lm column was used instead of 15 m60.15 mm60.15 lm LOQs Calculation of LOQs was also performed to validate the method. The values are summarized in Table 2 for all tested pesticides and sample preparation methods. LOQ was determined as the concentration of a compound that gives a response which relates to the S/N = 10 (calculated by MS software). In general the best LOQs were obtained for the modified Schenck s method. Notably for nonspecific masses a 170 m/z (see Fig. 1) LOQs obtained for the modified Schenck s method were lower than those for the other three methods (except dimethoate with m/z 87 in the case of modified QuEChERS). Modified QuEChERS method provides 21 72% better LOQs than the original QuEChERS for all pesticides analyzed (except myclobutanil). LOQs of MSPD in some cases exceeds the LOQs obtained for the modified Schenck s method in more than one order. On the contrary, especially at high Table 2. LOQs (lg/kg) Pesticide Modified Schenck s method Original QuEChERS Modified QuEChERS method MSPD Dimethoate Terbuthylazine Diazinon Pyrimethanil Chlorpyrifos-methyl Fenitrothion Chlorpyrifos Cyprodinyl Penconazole Methidathion Kresoxim-methyl Myclobutanil Tebuconazole Phosalone Bitertanol Bitertanol Cypermethrin Cypermethrin Cypermethrin Etofenprox masses even lower LOQs compared with Schenck s method are obtained. 3.3 Application to real-life samples Apple samples (cultivar Topaz) obtained from suppliers from south-west Slovakia (raw material to the producer of baby food) were analyzed by three methods combined with fast GC-MS (Table 3). Pesticide etofenprox was found at the level exceeding MRL. The result obtained using the modified Schenck s method was lower compared to QuE- ChERS methods, but the maximal difference did not exceed 20%. Two test portions were taken and analyzed in three GC measurements to check the repeatability. Excellent repeatability (RSDs f 2.2%) was obtained. As another application the modified QuEChERS procedure was used for tracing the fate of pesticides during the technological process of baby food production. Also the raw apples (cultivar Jonatan) from a relevant consignment were sampled and analyzed for the comparison. Two pesticides, fenitrothion and pyrimethanil were detected and quantified after each step and also in raw apples. The results are presented in Table 4. The concentration of both pesticide residues decreases after the first steps. A probable explanation of this could be the degradation of the residues after the first heating step. Differences in the next steps are not significant, although a slight decrease of concentration was observed in general. 4 Concluding remarks Four tested sample preparation methods combined with fast GC-MS analysis reached the requirements of EU legislation on R, repeatability, [16] and LOQs for baby food [1]. Modified QuEChERS method utilizing column-based SPE clean-up instead of dispersive SPE provides lower recoveries, but slightly better LOQs. Modified Schenck s
8 J. Sep. Sci. 2006, 29, Sample preparation methods fast GC-MS for pesticide residues analysis 1109 Table 4. Pyrimethanil and fenitrothion content determined by the modified QuEChERS method connected with fast GC-MS in the samples collected after each step of the technological process of baby food production and in raw apples (step 0) Traced pesticide Step of the technological process Average concentration (lg/kg) Pyrimethanil RSD PA % b) RSD GC % Average concentration (lg/kg) Fenitrothion RSD PA % RSD GC b) % n =2. b) n =3. method with the best cleaning efficiency provides the lowest LOQs, while MSPD provides the worst cleaning related to the highest LOQs. QuEChERS method without any evaporation step offers extreme improvement in rapidity and simplicity in comparison with the other methods tested. It makes the QuEChERS method most attractive and useful in the ultratrace analysis of pesticides. The fast GC-MS technique using a quadrupole analyzer has been shown to be a valuable technique in pesticide residue analysis of complex mixtures and complex matrix, providing faster analytical answers with higher sensitivity compared to conventional capillary GC-MS. The authors gratefully acknowledge the support of a part of this research within the framework of the Slovak Grant Agency (VEGA, project no. 1/2463/05) and NATO project no. SfP References [1] Commission Directive 1999/39/EC amending Directive 95/5/EC, Off. J. Eur. Com. 1999, L 124/8. [2] Hercegovµ, A., Dömötörovµ, M., Matisovµ, E., Kirchner, M., et al., J. Chromatogr. A 2005, 1084, [3] Dömötörovµ, M., Matisovµ, E., Kirchner, M., de Zeeuw, J., Acta Chim. Slov. 2005, 52, [4] Anastassiades, M., Lehotay, S. J., ƒtajnbaher, D., Schenck, F. J., J. AOAC Int. 2003, 86, [5] Lehotay, S. J., de Kok, A. D., Hiemstra, M., van Bodegraven, P., J. AOAC Int. 2005, 88, [6] Leandro, C. C., Fussell, R. J., Keely, B. J., J. Chromatogr. A 2005, 1085, [7] Matisovµ, E., Dömötörovµ, M., J. Chromatogr. A 2003, 1000, [8] Kirchner, M., Matisovµ, E., Hrouzkovµ, S., de Zeeuw, J., J. Chromatogr. A 2005, 1090, [9] Kirchner, M., Matisovµ, E., Otrekal, R., Hercegovµ, A., de Zeeuw, J., J. Chromatogr. A 2004, 1084, [10] Klee, M. S., Blumberg, L. M., J. Chromatogr. Sci. 2002, 40, [11] Dömötörovµ, M., Kirchner, M., Matisovµ, E., de Zeeuw, J., J. Sep. Sci., DOI: /jssc [12] HajÐlovµ, J., Zrostlíkovµ, J., J. Chromatogr. A 2003, 1000, [13] Kirchner, M., Matisovµ, E., Dömötörovµ, M., de Zeeuw, J., J. Chromatogr. A 2004, 1055, [14] Kružlicovµ, D., Thesis, Slovak University of Technology, Bratislava [15] Lehotay, S. J., MaÐtovskµ, K., Lightfield, A. R., J. AOAC Int. 2005, 88, [16] Council Directives 94/43/EC, Off. J. Eur. Com., 1994, L [17] Eckschlager, K., Horsµk, I., KodejÐ, Z., in: Hugovµ, E. (Eds.), Evaluation of Analytical Results and Methods, SNTL, Praha 1980, p. 25.
Highly Sensitive and Rugged GC/MS/MS Tool
Highly Sensitive and Rugged GC/MS/MS Tool For Pesticide Multiresidue Analysis in Food Samples Agilent 7 Series Triple Quadrupole GC/MS. The world s first MS/MS designed specifically for GC Analysis Introduction
More informationMSPD Combined With Fast GC for Ultratrace Analysis of Pesticide Residues in Non-Fatty Food
4 Acta Chim. Slov.,, 4 48 Scientific Paper MSPD Combined With Fast GC for Ultratrace Analysis of Pesticide Residues in Non-Fatty Food Milena Dömötörová, a Eva Matisová, a * Michal Kirchner, a and Jaap
More informationAnaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection
Anaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection Application Note Food Safety Author Andy Zhai Agilent technologies Co., Ltd. 412 Yinglun Road Shanghai,
More informationThermo Scientific HyperSep Dispersive SPE Products. Efficient sample preparation and clean-up using the QuEChERS Method
Thermo Scientific HyperSep Dispersive SPE Products Efficient sample preparation and clean-up using the QuEChERS Method The QuEChERS method overcomes the problems associated with time consuming, expensive
More informationMulti-residue GC-MS analysis. Richard Fussell CSL York, UK
Multi-residue GC-MS analysis Richard Fussell CSL York, UK e-mail: r.fussell@csl.gov.uk Outline of Presentation Background Gas Chromatography Mass Spectrometry - Options Capabilities and Applications Summary
More informationInstitute of Plant Protection Belarusian State University
Institute of Plant Protection Belarusian State University ovel method of determination of pesticides in rapeseed oil based on dissociation extraction in water-free medium followed by gas chromatography/mass
More informationPesticides Analysis Using the Agilent 5977A Series GC/MSD
Pesticides Analysis Using the Agilent 5977A Series GC/MSD Application Note Food Testing and Agriculture Authors Jia-jia Wu and Wen-wen Wang Agilent Technologies Co. Ltd (China) Beijing, People s Republic
More informationValidation Report 18
EURL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Validation Report 18 Determination of pesticide residues in maize for livestock feed by GC-MS/MS and LC-MS/MS
More informationApplication Note No Introduction. DMI Instrumentation. DMI Method. DMI for Lettuce and Pea Extracts. Lettuce results
Application Note No. 082 Multi-Residue Analysis of Pesticides in Samples of Lettuce and Peas Using Large Volume-Difficult Matrix Introduction-Gas Chromatography-Mass Spectrometry (LV-DMI-GC-MS) Richard
More informationLC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges
LC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges Application ote Food Testing & Agriculture Author Andy Zhai Agilent Technologies Shanghai Co. Ltd. Abstract This application
More informationIMPROVING PESTICIDE RESIDUE DETECTION PROTOCOL FOR FRUIT
IMPROVING PESTICIDE RESIDUE DETECTION PROTOCOL FOR FRUIT Su Myeong Hong National Academy of Agricultural Science, Rural Development Administration (RDA), Wanju, Korea e-mail: wideyun@korea.kr ABSTRACT
More informationValidation Report Determination of isoprothiolane residues in rice by GC-MS/MS (QuEChERS method)
NRL for Cereals DTU National Food Institute Validation Report Determination of isoprothiolane residues in rice by GC-MS/MS (QuEChERS method) Mette Erecius Poulsen & Hanne Bjerre Christensen 16 July 21
More informationMulti-residue analysis of pesticides by GC-HRMS
An Executive Summary Multi-residue analysis of pesticides by GC-HRMS Dr. Hans Mol is senior scientist at RIKILT- Wageningen UR Introduction Regulatory authorities throughout the world set and enforce strict
More informationAnalysis of pesticide residues using various GC/MS systems. Radim Štěpán Czech Agriculture and Food Inspection Authority Prague, Czech Republic
Analysis of pesticide residues using various GC/MS systems Radim Štěpán Czech Agriculture and Food Inspection Authority Prague, Czech Republic OUTLINE Czech Agriculture and Food Inspection Authority (CAFIA)
More informationDetermination of Pesticide Residues in Oats by Automated. QuEChERS and LC/QQQ. Application Note. Abstract. Introduction
Determination of Pesticide Residues in Oats by Automated QuEChERS and LC/QQQ Application Note Abstract The QuEChERS (Quick-Easy-Cheap-Effective-Rugged-Safe) sample extraction method was developed for the
More informationFully automated QuEChERS clean-up and LC/MS-QQQ analysis of pesticides in fruits and vegetables.
Chromatography Technical Note No AS90 Fully automated QuEChERS clean-up and LC/MS-QQQ analysis of pesticides in fruits and vegetables. Paul H. Roberts, Anatune Ltd. Hardwick, Cambridgeshire, UK. Introduction
More informationApplication Note. Abstract. Introduction. Determination of Polycyclic Aromatic Hydrocarbons in Seafood by an Automated QuEChERS Solution
Application Note Abstract Determination of Polycyclic Aromatic Hydrocarbons in Seafood by an Automated QuEChERS Solution Page 1 Polycyclic Aromatic Hydrocarbons (PAHs) are a large group of organic compounds
More informationAvailable online Journal of Chemical and Pharmaceutical Research, 2012, 4(6): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(6):3275-3279 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and validation of a matrix solid-phase
More informationSulfotepp impurities in Chlorpyrifos EC formulations
Page 1 of 16 Method DAS-AM-01-058 Sulfotepp impurities in Chlorpyrifos EC formulations A. ABSTRACT Method DAS-AM-01-058 has been validated for the analysis of the relevant impurity sulfotepp in chlorpyrifos
More informationDETERMINATION OF OCs, PCBs AND SYNTHETIC PYRETHROIDS IN ANIMAL FAT
DETERMINATION OF OCs, PCBs AND SYNTHETIC PYRETHROIDS IN ANIMAL FAT Peter Hancock 1, Mike Hetmanski 2 and Richard J. Fussell 2 1 Waters Corporation, Atlas Park, Simonsway, Manchester M22 5PP, UK 2 Central
More informationMacrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS
Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS Application Note Food Testing and Agriculture Author Chen-Hao (Andy) Zhai and Rong-jie Fu Agilent Technologies (Shanghai)
More informationApplication Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors
Trace-Level Analysis of Melamine in Milk Products on Agilent 789A/5975C GC/MSD Using a ew Agilent J&W DB-5ms Ultra Inert Column and SampliQ SCX Cartridges Application ote Gas Chromatography/Mass Spectrometry/Food
More informationRapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry
Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Application Note Food Authors Jianqiu Mi, Zhengxiang Zhang, Zhixu Zhang,
More informationDETERMINATION OF PESTICIDES IN FOOD USING UPLC WITH POLARITY SWITCHING TANDEM QUADRUPOLE LC/MS/MS
DETERMINATION OF PESTICIDES IN FOOD USING UPLC WITH POLARITY SWITCHING TANDEM QUADRUPOLE LC/MS/MS Peter Hancock 1, Cristiana C. Leandro 2,3, Brendan J. Keely 2 and Richard J. Fussell 3 1 Waters Corporation,
More informationMethod for the determination of dimethyl sulfate
German Social Accident Insurance Deutsche Gesetzliche Unfallversicherung Analytical Subcommittee of the Chemistry Board of Experts* Carcinogenic substances Order number: BGI 505-7-05 Established methods:
More informationAminoglycosides in Milk Using Agilent Bond Elut Plexa SPE, Agilent Poroshell 120, and LC/Tandem MS
Aminoglycosides in Milk Using Agilent Bond Elut Plexa SPE, Agilent Poroshell 120, and LC/Tandem MS Application ote Food Testing & Agriculture Author Andy Zhai Agilent Technologies, Inc. Shanghai Co. Ltd.
More informationYokogawa Analytical Systems Inc., Nakacho, Musashino-shi, Tokyo , Japan.
Determination of organochlorine pesticides in river water by gas chromatography-negative-ion chemical-ionization mass spectrometry using large volume injection Sadao Nakamura,* Takashi Yamagami and Shigeki
More informationSimultaneous Estimation of Residual Solvents (Isopropyl Alcohol and Dichloromethane) in Dosage Form by GC-HS-FID
Asian Journal of Chemistry Vol. 21, No. 3 (2009), 1739-1746 Simultaneous Estimation of Residual Solvents (Isopropyl Alcohol and Dichloromethane) in Dosage Form by GC-HS-FID PRAVEEN KUMAR BALIYAN*, R.P.
More informationTyler Trent, Applications Sales Specialist, Teledyne Tekmar P a g e 1
Application Note AutoMate-Q40 Automated QuEChERS Extraction for Pesticide Residues in Botanicals Tyler Trent, Applications Sales Specialist, Teledyne Tekmar P a g e 1 Abstract QuEChERS is a Quick-Easy-Cheap-
More informationThe Quantitation and Identification of Coccidiostats in Food by LC-MS/MS using the AB SCIEX 4000 Q TRAP System
The Quantitation and Identification of Coccidiostats in Food by LC-MS/MS using the AB SCIEX 4000 Q TRAP System Bertram ieland 1 and Stephen Lock 2 1 AB SCIEX ieuwerkerk aan den Ijssel, The etherlands;
More informationSTANDARD OPERATING PROCEDURES SOP: 1828 PAGE: 1 of 14 REV: 0.0 DATE: 05/12/95 ANALYSIS OF METHYL PARATHION IN CARPET SAMPLES BY GC/MS
PAGE: 1 of 14 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY CONTENTS 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS
More informationValidation Report 8 Determination of pesticide residues in rice and wheat by GC-MS/MS and LC-MS/MS (QuEChERS method) Appendix 2
EURL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Validation Report 8 Determination of pesticide residues in rice and wheat by GC-MS/MS and LC-MS/MS (QuEChERS method)
More informationSample Prep Options for GC users
Sample Prep Options for GC users Tina Chambers Technical Specialist Sample Preparation tina.chambers@agilent.com 949-842-0493 Why Bother? Removal of interferences which would otherwise affect detection
More informationIdentification and Quantitation of Pesticides in Chamomile and Ginger Extracts Using an Agilent 6460 Triple Quadrupole LC/MS system with Triggered MRM
Identification and Quantitation of Pesticides in Chamomile and Ginger Extracts Using an Agilent 646 Triple Quadrupole LC/MS system with Triggered MRM Application Note Authors Thomas Glauner Agilent Technologies,
More informationApplication Note. Abstract. Authors. Introduction. Food Safety
Ultra Inert (UI) Wool Liner Performance Using an Agilent J&W DB-35ms UI Column with and without an Analyte Protectant for Organophosphorus (OP) Pesticides Application Note Food Safety Authors Ken Lynam
More informationDetermination of Pesticides in Aqueous Samples by On-Line Coupling Solid-Phase Extraction to Gas Chromatography with At-Column Concentrating Interface
Application Note No. 033 Determination of Pesticides in Aqueous Samples by On-Line Coupling Solid-Phase Extraction to Gas Chromatography with At-Column Concentrating Interface Ryoichi Sasano*, Takayuki
More informationTrace analysis of mesityl oxide and diacetone alcohol in pharmaceuticals by capillary gas chromatography with flame ionization detection
Trade Science Inc. September 2009 Volume 8 Issue 3 ACAIJ, 8(3) 2009 [346-349] Trace analysis of mesityl oxide and diacetone alcohol in pharmaceuticals by capillary gas chromatography with flame ionization
More informationValidation Report 3 Determination of pesticide residues in cereals by GC-MS/MS (QuEChERS method)
CRL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Validation Report 3 Determination of pesticide residues in cereals by GC-MS/MS (QuEChERS method) Susan Strange
More informationAnalysis of Trace (mg/kg) Thiophene in Benzene Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application
Analysis of Trace (mg/kg) Thiophene in Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application Petrochemical Authors James D. McCurry and Bruce D. Quimby Agilent Technologies
More informationTHE NEW QUANTITATIVE ANALYTICAL METHOD FOR ULTRATRACE SULFUR COMPOUNDS IN NATURAL GAS
International Gas Union Research Conference 14 THE NEW QUANTITATIVE ANALYTICAL METHOD FOR ULTRATRACE SULFUR COMPOUNDS IN NATURAL GAS Main author Hironori IMANISHI Tokyo Gas Co., Ltd. JAPAN himanishi@tokyo-.co.jp
More informationApplication Note GCMS-01 Comparison of Ionization Techniques for the Analysis of Trace-Level Pyrethroid Insecticides by GC-MS/MS
Application Note GCMS-01 Comparison of Ionization Techniques for the Analysis of Trace-Level Pyrethroid Insecticides by GC-MS/MS Abstract A study was carried out using the Bruker EVOQ GC-TQ to screen a
More informationDetermination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS
Determination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS Application Note Food Authors Gerd Vanhoenacker, Frank David,
More informationInternational Journal of Research in Pure and Applied Chemistry
Available online at http://www.urpjournals.com International Journal of Research in Pure and Applied Chemistry Universal Research Publications. All rights reserved Original Article Simultaneous determination
More informationPerfluorinated Alkyl Acids (PFAA) in Water by LC/MS/MS - PBM
Organics Revision Date: July 19, 2017 Perfluorinated Alkyl Acids (PFAA) in Water by LC/MS/MS - PBM Parameter Perfluorinated Alkyl Acids (Perfluorobutane Sulphonate (PFBS), Perflourooctane Sulphonate (PFOS),
More informationAnalysis of Illegal Dyes in Food Matrices using Automated Online Sample Preparation with LC/MS
Application Note: 56 Analysis of Illegal Dyes in Food Matrices using Automated Online Sample Preparation with LC/MS Yang Shi, Catherine Lafontaine, Matthew Berube, John Fink, François Espourteille Thermo
More informationSTANDARD OPERATING PROCEDURES
PAGE: 1 of 12 CONTENTS 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING, AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS
More informationAnalysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ8030. No. GCMS No. SSI-GCMS-1404
Gas Chromatograph Mass Spectrometer No. GCMS-1404 Analysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ8030 Introduction Contamination of consumer products with pesticides is a growing concern
More informationQuantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS
Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1
More informationHICHROM. Chromatography Columns and Supplies. SAMPLE PREPARATION Solid Phase Extraction - SOLA & HyperSep. Catalogue 9.
HICHROM Chromatography Columns and Supplies SAMPLE PREPARATION Solid Extraction - SOLA & HyperSep Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)8 930 3660
More information[ Care and Use Manual ]
Oasis HLB DISKS I. Introduction Oasis HLB is a Hydrophilic-Lipophilic-Balanced, water-wettable, reversed-phase sorbent for all your SPE needs. It is made from a specific ratio of two monomers, the hydrophilic
More informationScreening of Pesticide Residues in Water by Sequential Stir Bar Sorptive Extraction-Thermal Desorption with GC/MS
Screening of Pesticide Residues in Water by Sequential Stir Bar Sorptive Extraction-Thermal Desorption with GC/MS Application Note Food Authors Nobuo Ochiai and Kikuo Sasamoto GERSTEL K.K. 2-13-18 Nakane
More informationDETERMINATION OF CHLORAMPHENICOL RESIDUES IN HONEY BY MONOLITHIC COLUMN LIQUID CHROMATOGRAPHY MASS SPECTROMETRY AFTER USE OF QUECHERS CLEAN-UP
ACTA CHROMATOGRAPHICA, NO. 17, 2006 DETERMINATION OF CHLORAMPHENICOL RESIDUES IN HONEY BY MONOLITHIC COLUMN LIQUID CHROMATOGRAPHY MASS SPECTROMETRY AFTER USE OF QUECHERS CLEAN-UP C. Pan 1,*, H. Zhang 2,
More informationValidation of a Simple and Rapid Multiresidue Method (QuEChERS) and its Implementation in Routine Pesticide Analysis
Validation of a Simple and Rapid Multiresidue Method (QuEChERS) and its Implementation in Routine Pesticide Analysis Michelangelo Anastassiades, Ellen Scherbaum and Dorothea Bertsch Chemisches und Veterinäruntersuchungsamt
More informationAnalysis of Phthalates in Body Wash using Solid-Supported Liquid-Liquid Extraction
Analysis of Phthalates in Body Wash using Solid-Supported Liquid-Liquid Extraction Application Note Consumer Products Authors Devon C. Zimmerman, Henry F. Rossi III, Jacqueline Rizzo, Daniel W. Keating,
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE
Translated English of Chinese Standard: GB5009.28-2016 www.chinesestandard.net Buy True-PDF Auto-delivery. Sales@ChineseStandard.net NATIONAL STANDARD OF THE GB PEOPLE S REPUBLIC OF CHINA GB 5009.28-2016
More informationHPLC Winter Webinars Part 2: Sample Preparation for HPLC
HPLC Winter Webinars Part 2: Sample Preparation for HPLC Jon Bardsley, Application Chemist Thermo Fisher Scientific, Runcorn/UK The world leader in serving science What am I Going to Talk About? What do
More informationLimit-test of NDMA and NDEA in Sartans. by GC-MS (Liquid-direct-injection)
Limit-test of NDMA and NDEA in Sartans 1 Purpose and scope by GC-MS (Liquid-direct-injection) The method is used to test the permissible limits of N-nitrosodimethylamine and N- nitrosodiethylamine in various
More informationDEVELOPMENT OF HPLC METHOD FOR ANALYSIS OF NITRITE AND NITRATE IN VEGETABLE
Journal of Agricultural, Food and Environmental Sciences UDC 635.546.173/.175]:543.544.5.068.7 Original scientific paper DEVELOPMENT OF HPLC METHOD FOR ANALYSIS OF NITRITE AND NITRATE IN VEGETABLE A. Najdenkoska*
More information[application note] INTRODUCTION EXPERIMENTAL. Specimens. Extraction
Testing for GHB in hair by GC tandem quadrupole MS Marie Bresson, Vincent Cirimele, Pascal Kintz, Marion Villain; Laboratoire Chemtox, Illkirch, France Timothy Jenkins, Waters Corporation, Manchester,
More informationENVIRONMENTAL analysis
ENVIRONMENTAL analysis Analyzing Wastewater Effluents for PAH s and PBDE s Using the Agilent 7000 Triple Quadrupole GC/MS Solutions for Your Analytical Business Markets and Applications Programs Authors
More informationMethod - Determination of aromatic amines in mainstream cigarette smoke
British American Tobacco Group Research & Development Method - Determination of aromatic amines in mainstream cigarette smoke 1 SCOPE OF APPLICATION The method is applicaple to quantitative determination
More informationAssessment of Pesticide Residues in Some Fruits Using Gas Chromatography Coupled with Micro Electron Capture Detector
ISSN-1996-918X Pak. J. Anal. Environ. Chem. Vol. 12, No. 1 & 2 (2011) 76-87 Assessment of Pesticide Residues in Some Fruits Using Gas Chromatography Coupled with Micro Electron Capture Detector Yawar Latif,
More informationDetermination of Off-Odor Compounds in Drinking Water Using an SPME Device with Gas Chromatography and Mass Spectrometry
Application Note Environmental Determination of Off-Odor Compounds in Drinking Water Using an SPME Device with Gas Chromatography and Mass Spectrometry Authors Ye Kong and Zhe Cao Agilent Technologies,
More informationH 3 CO H 3 CO S CH 3
FENITROTHION 35 H 3 CO P H 3 CO S O CH 3 NO 2 ISO common name Chemical name Fenitrothion O,O-Dimethyl O-4-nitro-m-tolyl phosphorothioate (IUPAC) O,O-Dimethyl O-(3-methyl-4-nitrophenyl)- phosphorothioate
More informationOrganophosphorus Pesticides in Apple Matrix by GC/MS/FPD using an Agilent J&W DB-35ms Ultra Inert GC Column
Organophosphorus Pesticides in Apple Matrix by GC/MS/FPD using an Agilent J&W DB-5ms Ultra Inert GC Column Application Note Environmental and Food Safety Author Doris Smith and Ken Lynam Agilent Technologies,
More informationHigh Efficiency, Broad Scope Screening of Pesticides Using Gas Chromatography High Resolution Orbitrap Mass Spectrometry
High Efficiency, Broad Scope Screening of Pesticides Using Gas Chromatography High Resolution Orbitrap Mass Spectrometry Dominic Roberts,1 Hans Mol,2 Marc Tienstra,2 Cristian Cojocariu,1 and Paul Silcock1
More informationQuantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS
Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1
More informationDetermination of Elemental Sulfur in Explosives and. Explosive Residues by Gas Chromatography-Mass Spectrometry. Kimberly S.
Determination of Elemental Sulfur in Explosives and Explosive Residues by Gas Chromatography-Mass Spectrometry Kimberly S. Bradley Illinois State Police, Springfield Forensic Science Laboratory 24 Hill
More informationUCT. Selectively removing Chlorophyll without Compromising your Planar Pesticides INNOVATION THROUGH CHEMISTRY E N V I R O
E N V I R O Selectively removing Chlorophyll without Compromising your Planar Pesticides INNOVATION THROUGH CHEMISTRY ChloroFiltr is a polymeric sorbent available exclusively from. It is designed to replace
More informationAnalysis of Pesticide Residues in Apple using Agilent Bond Elut QuEChERS European Standard EN Kits by LC/MS/MS Detection
Analysis of Pesticide Residues in Apple using Agilent Bond Elut QuEChERS European Standard E Kits by LC/MS/MS Detection Application ote Food Safety Authors Limian Zhao, David Schultz, and Joan Stevens
More informationISSN: ; CODEN ECJHAO E-Journal of Chemistry , 7(2),
ISSN: 0973-4945; CODEN ECJHAO E-Journal of Chemistry http://www.e-journals.net 2010, 7(2), 629-635 Low-level Determination of Residual Methyl Methane Sulfonate and Ethyl Methane Sulfonate in Pharmaceuticals
More informationApplication Note. Author. Abstract. Food Safety. Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India
Analysis of fumonisin, FB1 and FB2 mycotoxins in corn food and feed samples using the Agilent 1120 Compact LC System coupled to the Agilent 6140 Single Quadrupole LC/MS System Application Note Food Safety
More informationMethod of determination of phtalates in spirituous beverages by gaschromatography/mass
OIV-MA-BS-33 Method of determination of phtalates in spirituous beverages by gas-chromatography/mass Type IV method 1. SCOPE OF APPLICATION This method applies to the detection and assay of some phthalates
More informationMETHOD 8032A ACRYLAMIDE BY GAS CHROMATOGRAPHY
METHOD 8032A ACRYLAMIDE BY GAS CHROMATOGRAPHY 1.0 SCOPE AND APPLICATION 1.1 Method 8032 is used to determine trace amounts of acrylamide monomer (CAS No. 79-06-1) in aqueous matrices. This method may be
More informationDetermination of trace anions in concentrated hydrofluoric acid
APPLICATION NOTE 78 Determination of trace anions in concentrated hydrofluoric acid Authors Archava Siriraks Thermo Fisher Scientific, Sunnyvale, CA Keywords HF, ICS-5000 +, IonPac AS10, IonPac AC10, ion
More informationChemical Analysis. Low Level Oxygenates Analyzer. Trace Analysis of Oxygenates in Hydrocarbon Matrices. Gas Chromatography.
Chemical Analysis Low Level Oxygenates Analyzer Trace Analysis of Oxygenates in Hydrocarbon Matrices think forward Gas Chromatography The determination of sub to high ppm levels of ethers, alcohols, aldehydes
More informationAdvantages of Reversed Sandwich Injection for Pesticide Residue Analysis
Advantages of Reversed Sandwich Injection for Pesticide Residue Analysis Application Note Author Jessica Westland Agilent Technologies, Inc. Abstract This application note demonstrates the use of the Agilent
More informationBritish American Tobacco Group Research & Development. Method - Determination of phenols in mainstream cigarette smoke
British American Tobacco Group Research & Development Method - Determination of phenols in mainstream cigarette smoke 1 SCOPE OF APPLICATION The method is applicable to the quantitative determination of
More informationAgilent Bond Elut QuEChERS Food Safety Applications Notebook: Volume 2 INNOVATIVE APPROACHES FOR TODAY S FOOD ANALYSIS CHALLENGES
Agilent Bond Elut QuEChERS Food Safety Applications Notebook: Volume 2 INNOVATIVE APPROACHES FOR TODAY S FOOD ANALYSIS CHALLENGES Table of Contents QuEChERS Page Food Safety Overview...3 What is QuEChERS?...4
More informationBRIEFING. Pharmacopeial Discussion Group Sign Off Document Attributes EP JP USP Definition Loss on drying Readily carbonizable substances
BRIEFING Saccharin, NF 22 page 2825 and page 1711 of PF 29(5) [Sept. Oct. 2003]. The United States Pharmacopeia is the coordinating pharmacopeia for the international harmonization of the compendial standards
More informationCH3 SO2NHCONH N N N. Bulletin of Environment, Pharmacology and Life Sciences. Original Article
Original Article Bulletin of Environment, Pharmacology and Life Sciences Online ISSN 2277 1808 Bull. Environ. Pharmacol. Life Sci.; Volume 1 [7] June 2012: 67-72 All Rights Reserved Academy for Environment
More informationPRINCIPLES AND APPLICATION OF CHROMATOGRAPHY. Dr. P. Jayachandra Reddy Mpharm PhD Principal & professor KTPC
PRINCIPLES AND APPLICATION OF CHROMATOGRAPHY Dr. P. Jayachandra Reddy Mpharm PhD Principal & professor KTPC CHROMATOGRAPHY Laboratory technique for the Separation of mixtures Chroma -"color" and graphein
More informationKatherine K. Stenerson, Michael Ye, Michael Halpenny, Olga Shimelis, and Leonard M. Sidisky. Supelco, Div. of Sigma-Aldrich Bellefonte, PA USA
New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis Katherine K.
More informationLC-MS/MS Method for the Determination of Diclofenac in Human Plasma
LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core
More informationDETERMINATION OF SOME SELECTED PESTICIDE RESIDUES IN APPLE JUICE BY SOLID - PHASE MICROEXTRACTION COUPLED TO GAS CHROMATOGRAPHY MASS SPECTROMETRY
ACTA UNIVERSITATIS AGRICULTURAE ET SILVICULTURAE MENDELIANAE BRUNENSIS Volume LIX 13 Number 1, 2011 DETERMINATION OF SOME SELECTED PESTICIDE RESIDUES IN APPLE JUICE BY SOLID - PHASE MICROEXTRACTION COUPLED
More informationValidation Report 5 Determination of pesticide residues in cereals by GC-MS/MS (QuEChERS method)
CRL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Validation Report 5 Determination of pesticide residues in cereals by GC-MS/MS (QuEChERS method) Susan Strange
More informationCHAPTER CHROMATOGRAPHIC METHODS OF SEPARATIONS
Islamic University in Madinah Department of Chemistry CHAPTER - ----- CHROMATOGRAPHIC METHODS OF SEPARATIONS Prepared By Dr. Khalid Ahmad Shadid Chemistry Department Islamic University in Madinah TRADITIONAL
More informationMETHOD 3600B CLEANUP
METHOD 3600B CLEANUP 1.0 SCOPE AND APPLICATION 1.1 Method 3600 provides general guidance on selection of cleanup methods that are appropriate for the target analytes of interest. Cleanup methods are applied
More informationSimultaneous determination of 74 kinds of pesticides in Chinese herbal medicine listed in Chinese pharmacopoeia (2015) by GPC-GC-MS/MS
PO-CON1555E Simultaneous determination of 74 kinds of pesticides in Chinese herbal medicine listed in Chinese pharmacopoeia (215) ASMS 215 ThP 34 Huiyan Yang, Jun Fan, Taohong Huang, Shin-ichi Kawano,
More informationComprehensive Pesticide Analysis in Juice Using a Combination of GC/MS and LC/MS Methods
Comprehensive Pesticide Analysis in Juice Using a Combination of GC/MS and LC/MS Methods Application Note Food Safety Authors Fadwa Al-Taher, Rima Juskelis, Yang Chen, Jack Cappozzo Institute for Food
More informationDetermination of Hormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell HPH Column (EPA 539)
Determination of ormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell P Column (EPA 539) Application Note Environmental Authors Rong-jie Fu and Chen-ao (Andy) Zhai Agilent Technologies
More information7. Stability indicating analytical method development and validation of Ramipril and Amlodipine in capsule dosage form by HPLC.
7. Stability indicating analytical method development and validation of and in capsule dosage form by HPLC. 7.1 INSTRUMENTS AND MATERIALS USED 7.1.1 INSTRUMENTS 1. Shimadzu LC-2010 CHT with liquid chromatograph
More informationMethod validation for determination of commonly used fungicides in rice and husk by gas liquid chromatography: Tandem mass spectrometry
2018; 6(3): 1264-1270 E-ISSN: 2320-7078 P-ISSN: 2349-6800 JEZS 2018; 6(3): 1264-1270 2018 JEZS Received: 21-03-2018 Accepted: 22-04-2018 Reena Chauhan Sushil Savita Rani MK Rana Nisha Kumari Department
More informationExtraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE. Myco Prior to LC-MS/MS Analysis
Application Note AN785 Extraction of Aflatoxins and chratoxin From Dried Chili Using ISLUTE Myco prior to LC-MS/MS Analysis Page Extraction of Aflatoxins and chratoxin from Dried Chili Using ISLUTE Myco
More informationDetermination of Volatile Substances Proof of Food Adulteration
ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of Volatile Substances Proof of Food Adulteration (method: gas chromatography with mass spectrometric detection) Exercise guarantor:
More informationApplication Note. Abstract. Introduction. Experimental-Instrument Conditions. By: Anne Jurek
Automated Handling Techniques for the Analysis of Elevated Volatile Organic Compound (VOC) Concentrations in Soils Utilizing the Atomx Concentrator/Multimatrix Autosampler. Application Note By: Anne Jurek
More informationAppendix II- Bioanalytical Method Development and Validation
A2. Bioanalytical method development 1. Optimization of chromatographic conditions Method development and optimization of chromatographic parameters is of utmost important for validating a method in biological
More informationDetermination of Aminoglycosides in animal tissue by means of LC/MS-MS
Austrian Agency for Health and Food Safety (AGES) Determination of Aminoglycosides in animal tissue by means of LC/MS-MS Created / revised Technical check QM check Release Name Thomas Aichinger Martin
More informationAuthor. Abstract. Introduction
Improved Performance for the Analysis of Aromatics in Gasoline by ASTM Method D5769 Using the Agilent 5973 inert Gas Chromatography/Mass Spectrometry System Application Author James D. McCurry Agilent
More informationFood & Environmental. Overview. Introduction. Method Details. André Schreiber and Yun Yun Zou SCIEX, Concord, Ontario, Canada
Food & Environmental Comprehensive Quantitation and Identification of Pesticides in Food Samples using LC-MS/MS with Scheduled MRM, Fast Polarity Switching, and MS/MS Library Searching André Schreiber
More information