Macromolecular Chemistry
|
|
- Junior Hensley
- 5 years ago
- Views:
Transcription
1 Macromolecular Chemistry Vacuum Degasser Pump and Pulse Controller Autosampler Solvent and Filter In-Line Filter Column Oven and Columns Injection Loop Sample Source Detector 1 Detector 2 Detector 3 Waste Lecture 9
2 Midterm Exam Where: Right here.in the lecture room WEL When: Next Tuesday 2/24/09 at 3:30 5PM What: Covers lectures through Thursday 2/18 Bring: Pencil, eraser, Calculator only closed book! Do: Study lecture notes, homework, reading assignments and graduate presentations Do not: Memorize the free radical kinetics equations but do know the principles! Go over homework problems. Please: Do a good job!
3 Measuring Molecular Weight Membrane Osmometry Alfredo Vapor Phase Osmometry Linda Viscometry GW Gel Permeation Chromatography Size exclusion Chromatography Light Scattering MALDI GW Others GW End group analysis You
4 MALDI Mass Spectrum of a Synthetic Polymer CH 3 -(CHO) n -OH+Na n
5 High Performance Liquid Chromatography Vacuum Degasser Pump and Pulse Controller Autosampler Solvent and Filter In-Line Filter Column Oven and Columns Injection Loop Sample Source Detector 1 Detector 2 Detector 3 Waste
6 Typical Instrument
7 Autosampler
8 Columns the heart of the tool
9 Reverse phase HPLC Partition Coefficient K = Solubility in mobile phase Solubility in stationary phase K > K
10 Column packing materials C-18 Silica gel
11 Refractive Index Detection Snell s Law
12 . HPLC
13
14 UV-Detector Absorbance time
15 Remarkable Separations! 2. O S NH 2 O
16 All of the amino acids resolved!
17 POLYMERS Size Exclusion Chromatography Gel Permeation Chromatograpy
18 Size Exclusion Chromatography
19
20
21
22 Calibration Run
23 Calculation of MW s Log (Molecular Weight) M i Number Average (Mn) (Weights Low Molecular Weights) Mn = ci c i / M i RI Height (Weight Fraction) c i Retention Volume Weight Average (Mw) (Average Molecular Weight) Mw Mi c = ci i
24 Setting the Standard for GPC. Advantages of Conventional Calibration 1) Simple Setup. (Only one Detector Required - RI or UV) 2) Solution Concentration Not a Variable. (Approximate Concentrations are Good Enough) 3) Excellent Precision (Repeatability) (Related to Column and Pump Performance) Disadvantages of Conventional Calibration 1) Mw Values Only Accurate for One Sample Type (Can Only Get True Molecular Weights of Polymers Which are the Same Type as the Calibrant.) 2) Ignores Structural Differences (Such as Branching, Aggregation and other structural modifications)
25 Setting the Standard for GPC. Overlay of Conventional Calibration Curves Each Polymer has a Unique Size to Weight Relationship. 6.5 Log (Molecular Weight) PS PAMS PMMA PIP PBD PPG Retention Volume (ml)
26 Setting the Standard for GPC. Classical Determination of MW Viscometry Common terms of of expressing viscosity Relative Viscosity η r =η/η 0 Reduced Viscosity η red =η sp /C Intrinsic Viscosity [η]=η sp /C 0 Specific Viscosity η sp = (η - η 0 ) /η 0 Inherent Viscosity η inh =lnη r /C
27 Setting the Standard for GPC. Intrinsic Viscosity (IV) is Determined By Extrapolating Ubbelohde Tube Measurements Down to Zero Concentration. η sp c GPC Concentration Range concentration GPC Measurements Are Made Two Orders of Magnitude Lower in Concentration Than Ubbelohde Measurements.
28 Setting the Standard for GPC. Interpretation of Intrinsic Viscosity Mark-Houwink Relationship The Mark-Houwink equation is an empirical equation relating intrinsic viscosity and molecular weight, and is generally valid for linear polymers, at least above 10,000 MW: [ η] = KM a
29 Setting the Standard for GPC. Universal Calibration and Differential Viscometry Developed by by W. W. Yau, Yau, Carrier Liquid Pump Bath Pulse Dampener Reference Capillary and Transducer Dilution Column Analytical Capillary and Transducer Sample Loop RI Detector DuPont PSM Columns Waste P R Differential Log Amplifier P A Dual Dual Capillary Viscometer log η rel
30 Setting the Standard for GPC. Detector Responses for Narrow Standards Detector Response (mv) Viscometer Polystyrene 850,000 Detector Responses VISC IV x C RI dn/dc x C Refractometer Polystyrene 30, Retention Volume (ml)
31
32
33 Grubisic, Rempp & Benoit, JPS Pt. B, 5, 753 (1967)
34 Setting the Standard for GPC. Universal Calibration All Polymers Fall on the Same IV x MW Curve. Log [η] M ELUTION VOLUME Universal Calibration was first demonstrated and proven by Benoit in 1967, who showed that a wide range of polymer structures eluted on the same calibration curve when the intrinsic viscosity is included. PS Comb PS PS Star Hetero-Graft Copolymer PolyMethylMethacrylate PolyVinylChloride Graft Copolymer: PS/PMMA PolyPhenylSiloxane Polybutadiene It does not matter whether the polymer is linear, branched, block copolymer, heterogeneous copolymer, or whatever...
35
36 Setting the Standard for GPC.
37 SEC Deficiencies 45 o C at 8 am and 50 o C at noon, etc? Non-size exclusion mechanisms: binding. Big, bulky and slow (typically 30 minutes/sample). Temperature/harsh solvents no fun. You learn nothing by calibrating. Columns are expensive, easily damaged. 37
Molecular weight of polymers. Molecular weight of polymers. Molecular weight of polymers. Molecular weight of polymers. H i
Gel Permeation Chromatography (GPC) : Size Exclusion Chromatography GPC : 1. Chromatogram (V R vs H) H i Detector response Baseline N i M i 130 135 140 145 150 155 160 165 Elution volume (V R ) (counts)
More information(3) A UNIVERSAL CALIBRATION FOR GEL PERMEATION CHROMATOGRAPHY. [TIM = 4 R3 gx POLYMER LETTERS VOL. 5, PP (1967)
POLYMER LETTERS VOL. 5, PP. 753-759 (1967) A UNIVERSAL CALIBRATION FOR GEL PERMEATION CHROMATOGRAPHY Gel permeation chromatography is one of the most powerful techniques for characterizing the polydispersity
More information[VIM = 4 R3 gx ( 3)
POLYMER LETTERS vol. 5, PP. 753-759 (1967) A UNIVERSAL CALIBRATION FOR GEL PERMEATION CHROMATOGRAPHY Gel permeation chromatography is one of the most powerful techniques for characterizing the polydispersity
More informationSem /2007. Fisika Polimer Ariadne L. Juwono
Chapter 8. Measurement of molecular weight and size 8.. End-group analysis 8.. Colligative property measurement 8.3. Osmometry 8.4. Gel-permeation chromatography 8.5. Ultracentrifugation 8.6. Light-scattering
More informationSetting the Standard for GPC. Complete Guide for GPC / SEC / GFC Instrumentation and Detection Technologies. The Right Instrument for Your Application
TM Setting the Standard for GPC Complete Guide for GPC / SEC / GFC Instrumentation and Detection Technologies The Right Instrument for Your Application Viscotek is the global leader in Gel Permeation /
More informationHow to use GPC/SEC for compositional analysis
How to use GPC/SEC for compositional analysis Determining the relative concentration of two components in a polymer sample MOLECULAR SIZE MOLECULAR STRUCTURE MOLECULAR WEIGHT Introduction Over the last
More informationMacromolecular Chemistry
Macromolecular Chemistry Lecture 8 Measuring Molecular Weight Membrane Osmometry Alfredo Vapor Phase Osmometry Linda Viscometry GW Gel Permeation Chromatography Size exclusion Chromatography Light Scattering
More informationPolymers Reactions and Polymers Production (3 rd cycle)
EQ, Q, DEQuim, DQuim nd semester 017/018, IST-UL Science and Technology of Polymers ( nd cycle) Polymers Reactions and Polymers Production (3 rd cycle) Lecture 5 Viscosity easurements of the viscosity
More informationPolymer analysis by GPC-SEC. Technical Note. Introduction
Polymer analysis by GPC-SEC Technical Note Introduction Gel Permeation Chromatography (GPC), also referred to as Size Exclusion Chromatography (SEC) is a mode of liquid chromatography in which the components
More informationAdvanced GPC. GPC On Tour, Barcelona, 28 th February The use of Advanced Detectors in GPC
Advanced GPC GPC On Tour, Barcelona, 28 th February 2012 The use of Advanced Detectors in GPC 1 What does Conventional GPC give? Molecular weight averages Relative to the standards used Mw Weight Average
More informationAn Introductions to Advanced GPC Solutions
An Introductions to Advanced GPC Solutions Alan Brookes Sales Manager GPC Instruments EMEAI 9 th April 2014 Agilent GPC/SEC Solutions 1 Introduction to Polymers Polymers are long chain molecules produced
More informationCharacterization of polyphenylene sulphide using the Agilent PL-GPC 220 High Temperature GPC System with triple detection
materials analysis Characterization of polyphenylene sulphide using the Agilent PL-GPC 220 High Temperature GPC System with triple detection Solutions for Your Analytical Business Markets and Applications
More informationGPC/SEC An essential tool for polymer analysis
GPC/SEC An essential tool for polymer analysis Ben MacCreath, PhD Product Manager GPC/SEC Instrumentation 26 th March 2013 Introduction to Polymers Where are they found? Polyolefins Engineering Polymers
More informationGPC / SEC Theory and Understanding
Dr. Jason S. Davies, Smithers Rapra, UK Gel permeation chromatography (GPC), also known as size exclusion chromatography (SEC) is a branch of liquid chromatography specifically concerned with characterisation
More informationChapter 14. Molar Mass Distribution.
Chapter 14. Molar Mass Distribution. Difficulty with M n and M w, etc. osome polymers are hard to describe from just M n, M w, etc. o Examples: Bimodal, multimodal, nonuniform, broad, etc. MWDs. oin early
More informationGel Permeation Chromatography
Gel Permeation Chromatography Polymers and Coatings Laboratory California Polytechnic State University Gel permeation chromatography (GPC) has become the most widely used technique for determination of
More informationProgress toward reliable NC molecular mass distribution by GPC
Progress toward reliable NC molecular mass distribution by GPC Dr E Stubbs Emma.Stubbs@awe.co.uk www.awe.co.uk British Crown Owned Copyright 2016/AWE Content Introduction to GPC New instrumentation Sample
More informationGel Permeation Chromatography
Gel Permeation Chromatography Polymers and Coatings Laboratory California Polytechnic State University San Luis Obispo, CA Gel permeation chromatography (GPC) has become the most widely used technique
More informationGel Permeation Chromatography Basics and Beyond eseminar March 13, Jean Lane Technical and Applications Support LSCA, Columns and Supplies
Gel Permeation Chromatography Basics and Beyond eseminar March 13, 2013 Jean Lane Technical and Applications Support LSCA, Columns and Supplies 1 Content Overview of GPC/SEC What is it? Why do we use it?
More informationCOURSE MATERIAL: Unit 3 (Part 1) Polymer Science LT8501 (Click the link Detail to download)
COURSE MATERIAL: Unit 3 (Part 1) Polymer Science LT8501 (Click the link Detail to download) Dr. Debasis Samanta Senior Scientist & AcSIR Assistant Professor Polymer Science & Technology Department., CSIR-CLRI,
More informationApplication compendium. Authors. Greg Saunders, Ben MacCreath Agilent Technologies, Inc. A guide to multi-detector gel permeation chromatography
Application compendium Authors Greg Saunders, Ben MacCreath Agilent Technologies, Inc. A guide to multi-detector gel permeation chromatography Contents Introduction...3 Why do multi-detector GPC/SEC?...4
More informationDifferentiation of polymer branching and composition using the Mark Houwink plot
Differentiation of polymer branching and composition using the Mark Houwink plot MOLECULAR SIZE MOLECULAR STRUCTURE MOLECULAR WEIGHT Introduction The manipulation of polymer properties through changes
More informationGPC/SEC Practical Tips and Tricks. Thomas Dent Applications Scientist Agilent Technologies. October, 2011 Gulf Coast Conference
GPC/SEC Practical Tips and Tricks Thomas Dent Applications Scientist Agilent Technologies October, 2011 Gulf Coast Conference 1 Section 1: Introduction Goals Brief introduction to GPC/SEC Highlight considerations
More informationQuick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE
Quick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE Introduction Gel permeation chromatography (GPC) and size exclusion chromatography
More informationGPC - Gel Permeation Chromatography. aka Size Exclusion Chromatography- SEC
GPC - Gel Permeation Chromatography aka Size Exclusion Chromatography- SEC Wendy Gavin Biomolecular Characterization Laboratory Version 1 May 2016 1 Table of Contents 1. GPC Introduction. Page 3 2. How
More informationInstruction for practical work No 2. The Determination of Viscosity-Average Molecular Weight of Polymers
Instruction for practical work No 2 The Determination of Viscosity-Average Molecular Weight of Polymers THEORETICAL PART Molecular weight of polymers Molecular weight is one of the most fundamental parameters
More informationMacromolecular Chemistry
Macromolecular Chemistry N N N Cu + BR - N Lecture 7 Decomposition of Thermal Initiator k d I 2 R Efficiency factor ( f ): CN N N CN di-tert-butylperoxide AIBN di-tert-butylperoxalate f = 0.65 f = 0.75
More informationLatest Developments in GPC Analysis of Adhesive and Sealant Polymers Mark Pothecary PhD Americas Product Manager Malvern Instruments
Latest Developments in GPC Analysis of Adhesive and Sealant Polymers Mark Pothecary PhD Americas Product Manager Malvern Instruments Molecular weight The most fundamental molecular property that controls
More informationAnalysis of Star Polymers Using the Agilent 1260 Infinity Multi-Detector GPC/SEC System
Analysis of Star Polymers Using the Agilent 1260 Infinity Multi-Detector GPC/SEC System Application Note Material testing Authors Kayleigh McEwan, Rajan K. Randev, and David M. Haddleton University of
More informationSetting the Standard for Advanced GPC/SEC Systems
Absolute molecular weight Molecular conformation Molecular size Solution viscosity Viscotek Setting the Standard for s ṙ ṙ Detailed specification sheets from Introducing the system - the leading solution
More informationLecture 4 : Gel Permeation or Size Exclusion Chromatography
Lecture 4 : Gel Permeation or Size Exclusion Chromatography Polymer Fractionation Sedimentation Centrifugation Evaporation of the solvent Gel permeation chromatography Gel Permeation Chromatography (GPC)
More informationThe Differential Viscometer. 11. On-Line Viscosity Detector for Size-Exclusion Chromatography
The Differential Viscometer. 11. On-Line Viscosity Detector for Size-Exclusion Chromatography MAX A. HANEY, Viscotek Corporation, 1030 Russell Dr., Porter, Texas 77365 Synopsis The new technique of differential
More informationTips & Tricks GPC/SEC: From a Chromatogram to the Molar Mass Distribution
Tips & Tricks GPC/SEC: From a Chromatogram to the Molar Mass Distribution Peter Kilz and Daniela Held, PSS Polymer Standards Service GmbH, Mainz, Germany. Molar masses cannot be measured directly by gel
More informationTips & Tricks GPC/SEC: Inter-Detector Delay
Tips & Tricks GPC/SEC: Inter-Detector Delay Daniela Held and Wolfgang Radke, PSS Polymer Standards Service GmbH, Mainz, Germany Combinations of detectors are often used in gel permeation chromatography/size-exclusion
More informationThe power of multi-detector SEC in the analysis of antibodies
The power of multi-detector SEC in the analysis of antibodies How OMNISEC can measure antibody absolute MW independent of size and structural changes MOLECULAR SIZE MOLECULAR STRUCTURE MOLECULAR WEIGHT
More informationEcoSEC - semi-micro GPC/SEC system for high throughput polymer analysis
EcoSEC - semi-micro GPC/SEC system for high throughput polymer analysis TOSOH BIOSCIENCE ABOUT TOSOH BIOSCIENCE GMBH Tosoh Bioscience is an acknowledged global leader in the field of liquid chromatography
More informationOptimization of PLA/PLGA molecular weight and structure to suit final application requirements
Optimization of PLA/PLGA molecular weight and structure to suit final application requirements MOLECULAR SIZE MOLECULAR STRUCTURE MOLECULAR WEIGHT Introduction The biodegradable and biocompatible polymers,
More informationDr. Christoph Johann Wyatt Technology Europe GmbH Copyright Wyatt Technology Europe GmbH All Rights reserved 1
Dr. Christoph Johann Wyatt Technology Europe GmbH 2010 Copyright Wyatt Technology Europe GmbH All Rights reserved 1 Introduction Overview The Nature of Scattered Light: Intensity of scattered light Angular
More informationPractical Steps in GPC Method Development
Practical Steps in GPC Method Development Jean Lane Applications Engineer LC Columns & Consumables Technical Support March 23,2016 1 Overview Solvent Criteria for Solvent Selection Polymer Standards -
More informationComparison of Polymer Separation by Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation
Comparison of Polymer Separation by Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation Stepan Podzimek, 1 Christoph Johann 2 1 SYNPO / University of Pardubice, Czech Republic, stepan.podzimek@synpo.cz
More informationMALVERN ADVANCED GPC/SEC DETECTORS SETTING THE STANDARD MOLECULAR WEIGHT MOLECULAR SIZE MOLECULAR STRUCTURE
MOLECULAR WEIGHT MOLECULAR SIZE MOLECULAR STRUCTURE MALVERN ADVANCED GPC/SEC DETECTORS SETTING THE STANDARD Setting the Standard WHAT CAN ADVANCED GPC/SEC DETECTION OFFER YOU? A modern research environment
More informationChap. 2. Molecular Weight and Polymer Solutions
Chap.. Molecular Weight and Polymer Solutions. Number Average and Weight Average Molecular Weight A) Importance of MW and MW Distribution M.W. physical properties As M.W., toughness, viscosity ) Optimum
More informationOpen Column Chromatography, GC, TLC, and HPLC
Open Column Chromatography, GC, TLC, and HPLC Murphy, B. (2017). Introduction to Chromatography: Lecture 1. Lecture presented at PHAR 423 Lecture in UIC College of Pharmacy, Chicago. USES OF CHROMATOGRAPHY
More informationSize-exclusion Chromatography as a Useful Tool for the Assessment of Polymer Quality and Determination of Macromolecular Properties
Size-exclusion Chromatography as a Useful Tool for the Assessment of Polymer Quality and Determination of Macromolecular Properties Peter Kilz, PSS Polymer Standards Service, 55023 Mainz, Germany 1. Introduction
More informationMalvern Macromolecular Solutions. Innovative Solutions in Material Characterization
Malvern Macromolecular Solutions Innovative Solutions in Material Characterization MALVERN HEADQUARTERS Malvern Instruments is a Spectris company. Innovative Solutions in Material Characterization Particle
More informationInvestigating the Relationship Between the Rheological Properties of Hyaluronic Acid and its Molecular Weight and Structure using Multidetector
Investigating the Relationship Between the Rheological Properties of Hyaluronic Acid and its Molecular Weight and Structure using Multidetector SEC and SEC-MALS Presented by Bassem Sabagh, PhD Technical
More informationGas Chromatography. Vaporization of sample Gas-solid Physical absorption Gas-liquid Liquid immobilized on inert solid
Gas Chromatography Vaporization of sample Gas-solid Physical absorption Gas-liquid Liquid immobilized on inert solid Principles Instrumentation Applications 18-1 Retention Volumes Volumes rather than times
More informationMaximizing Performance Through GPC Column Selection
Maximizing Performance Through GPC Column Selection What Are Polymers? Polymers are long chain molecules produced by linking small repeat units (monomers) together There are many ways to link different
More informationHow switching mobile phases can improve your OMNISEC results
How switching mobile phases can improve your OMNISEC results Analysis of ester- and acid-capped PLGA samples in mobile phases of DCM and THF MOLECULAR SIZE Introduction MOLECULAR Biodegradable, thermoplastic
More informationWaters GPC User Guide and Tutorial for Using the GPC in the Reynolds Research Group 2 nd Edition: April 2012
Waters GPC User Guide and Tutorial for Using the GPC in the Reynolds Research Group 2 nd Edition: April 2012 Georgia Institute of Technology School of Chemistry & Biochemistry School of Materials Science
More informationOptimizing GPC Separations
Optimizing GPC Separations Criteria for Solvent Selection True sample solubility (Polarity and Time dependant) Compatibility with columns Avoid non-size exclusion effects (eg adsorption by reverse phase
More informationGel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC)
Gel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC) Size Exclusion Chromatography (SEC) is a non-interaction based separation mechanism in which compounds are retained for different
More informationCHEM 429 / 529 Chemical Separation Techniques
CHEM 429 / 529 Chemical Separation Techniques Robert E. Synovec, Professor Department of Chemistry University of Washington Lecture 1 Course Introduction Goal Chromatography and Related Techniques Obtain
More informationPhysical Polymer Science Lecture notes by Prof. E. M. Woo adapting from: Textbook of Physical Polymer Science (Ed: L. H.
Caution: Contents of the lecture notes are copyrighted ( 有版權 Textbook of Physical Polymer Science). (Do not use outside the class room purposes without permission from the publisher.) Physical Polymer
More informationHPLC Background Chem 250 F 2008 Page 1 of 24
HPLC Background Chem 250 F 2008 Page 1 of 24 Outline: General and descriptive aspects of chromatographic retention and separation: phenomenological k, efficiency, selectivity. Quantitative description
More informationHigh Performance Liquid Chromatography
STANDARDBASE techniques: High Performance Liquid Chromatography Drenthe College, The Netherlands 1. Introduction HPLC. High Performance Liquid Chromatography High Performance Liquid Chromatography (HPLC)
More informationGPC/SEC standards. Product guide
GPC/SEC standards Product guide Contents Polymer standards for GPC/SEC 3 Agilent EasiVial 5 Agilent EasiCal 8 Polystyrene 9 Polymethylmethacrylate 11 Polyethylene glycol/oxide 12 Other polymer standards
More informationChem 230, Fall, 2014 Homework Set # 3 Short Answer SOLUTIONS
Chem 230, Fall, 2014 Homework Set # 3 Short Answer SOLUTIONS 1. List two advantages of temperature programming in GC. a) Allows separation of solutes with widely varying retention factors in a reasonable
More informationHigh Performance Liquid Chromatography
High Performance Liquid Chromatography What is HPLC? It is a separation technique that involves: Injection of small volume of liquid sample Into a tube packed with a tiny particles (stationary phase).
More informationChromatography- Separation of mixtures CHEM 212. What is solvent extraction and what is it commonly used for?
Chromatography- Separation of mixtures CHEM 212 What is solvent extraction and what is it commonly used for? How does solvent extraction work? Write the partitioning coefficient for the following reaction:
More informationMolecular Weights of Copolymers Obtained by Gel Permeation Chromatography Light Scattering
Chapter 1 olecular Weights of Copolymers Obtained by Gel Permeation Chromatography Light Scattering Downloaded via 148.251.232.83 on January 27, 2019 at 21:02:53 (UTC). See https://pubs.acs.org/sharingguidelines
More informationCh.28 HPLC. Basic types of Liquid Chromatography Partition (LLC) Adsorption (LSC) Ion Exchange (IC) Size Exclusion (SEC or Gel Chromatography)
Ch.28 HPLC 28.1 Basic types of Liquid Chromatography Partition (LLC) Adsorption (LSC) Ion Exchange (IC) Size Exclusion (SEC or Gel Chromatography) High Performance (Pressure) LC Glass column st.steel (high
More informationComprehensive Polymer Analysis Strategies
Comprehensive Polymer Analysis Strategies Mike Woodman, LC Applications Scientist 10 February, 2016 1 Review of GPC/SEC Polymer Analysis Portfolio The Agilent GPC/SEC portfolio is a complete GPC solution
More informationAbstract: An minimalist overview of chromatography for the person who would conduct chromatographic experiments, but not design experiments.
Chromatography Primer Abstract: An minimalist overview of chromatography for the person who would conduct chromatographic experiments, but not design experiments. At its heart, chromatography is a technique
More informationGPC/SEC standards. Product guide
GPC/SEC standards Product guide Contents Polymer standards for GPC/SEC 3 Agilent EasiVial 5 Agilent EasiCal 8 Polystyrene 9 Polymethylmethacrylate 11 Polyethylene glycol/oxide 12 Other polymer standards
More informationTips & Tricks GPC/SEC: Protein Analysis with Size-Exclusion Chromatography
Tips & Tricks GPC/SEC: Protein Analysis with Size-Exclusion Chromatography Daniela Held and Thorsten Hofe, PSS Polymer Standards Service GmbH, Mainz, Germany Gel permeation chromatography/size-exclusion
More informationIntroduction to Chromatographic Separations
Introduction to Chromatographic Separations Analysis of complex samples usually involves previous separation prior to compound determination. Two main separation methods instrumentation are available:
More informationHPLC. High Performance Liquid Chromatography (HPLC) Harris Chapter 25
High Performance Liquid Chromatography (HPLC) Harris Chapter 25 12/1/2005 Chem 253 - Chapter 25 1 HPLC Separation of nonvolatile or thermally unstable compounds. If the analyte/sample can be found to be
More informationLC III: HPLC. Originally referred to as High-Pressure Liquid Chromatography. Now more commonly called High Performance Liquid Chromatography
LC III: HPLC What is HPLC? Originally referred to as High-Pressure Liquid Chromatography Now more commonly called High Performance Liquid Chromatography In general: The instrument controlled version of
More informationBenefit of light scattering technologies (RALS/LALS/MALS) and multidetection characterization in life science research?
Benefit of light scattering technologies (RALS/LALS/MALS) and multidetection characterization in life science research? Bert Postma Business Support Separations and MicroCal Size Exclusion Chromatography
More informationPolymer Analysis by Gel Permeation Chromatography
Polymer Analysis by Gel Permeation Chromatography A Historical Perspective The development of polymer has had a profound effect on the modern world. These versatile materials are used in an extensive array
More informationDetermination of Polymer Modifier in Asphalt
Standard Method of Test for Determination of Polymer Modifier in Asphalt AASHTO Designation: T xxx-xx (2005) 1. SCOPE 1.1. This method of test is used to determine the polymer content of an asphalt sample.
More informationHPLC. GRATE Chromatography Lab Course. Dr. Johannes Ranke. September 2003
HPLC GRATE Chromatography Lab Course Dr. Johannes Ranke Organisation The groups Start at 9:00 am End at 18:00 pm at the latest Friday, 19th we will finish at 2:00 pm Thursday, 11th: Lecture at 08:15 am
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 1 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationMolecular Weight of Polymers *
OpenStax-CNX module: m43550 1 Molecular Weight of Polymers * Sehmus Ozden Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons Attribution License 3.0 1 Introduction
More informationCASE STUDY. Degradation of Polyethylene by FTIR and High Temperature GPC
Degradation of Polyethylene by FTIR and High Temperature GPC CASE STUDY Degradation of Polyethylene by FTIR and High Temperature GPC PROBLEM The objective of this analysis was to determine if thermal degradation
More informationChromatography. Gas Chromatography
Chromatography Chromatography is essentially the separation of a mixture into its component parts for qualitative and quantitative analysis. The basis of separation is the partitioning of the analyte mixture
More informationField-Flow Fractionation of Macromolecules and Structures That Cannot be Characterized by Conventional GPC/SEC Techniques
The Field-Flow Fractionation Platform Field-Flow Fractionation of Macromolecules and Structures That Cannot be Characterized by Conventional GPC/SEC Techniques Trevor Havard, Evelin Moldenhaur, Soheyl
More informationc) fitting of the NMR intensity in dependence of the recycle delays 4
Supporting Information Prediction of NMR magnetization for onflow experiments: According to Albert, the relaxation rate can be expressed under flow conditions as follows: T flow = T + τ (S-) with T as
More informationGPC/SEC Analysis of Polymer Degradation
MOLECULAR WEIGHT Introduction MOLECULAR Understanding the effects of processing a polymer on its molecular weight, STRUCTURE molecular weight distribution, and solution viscosity are important to MOLECULAR
More informationGPC/SEC Analysis of Polymer Solutions Used in Inkjet Printing
GPC/SEC Analysis of Polymer Solutions Used in Inkjet Printing Introduction Inkjet printing of polymer solutions is an area of interest for depositing precise, high-throughput patterns of functional polymer.
More informationTips & Tricks GPC/SEC: Quantify and Get More Than Molar Mass Averages
Tips & Tricks GPC/SEC: Quantify and Get More Than Molar Mass Averages Daniela Held, PSS Polymer Standards Service GmbH, Mainz, Germany Gel permeation chromatography/size-exclusion chromatography (GPC/SEC)
More informationChapter 3. Molecular Weight. 1. Thermodynamics of Polymer Solution 2. Mol Wt Determination
Chapter 3 Molecular Weight 1. Thermodynamics of Polymer Solution 2. Mol Wt Determination 1. Weight, shape, and size of polymers monomer oligomer polymer dimer, trimer, --- telomer ~ oligomer from telomerization
More informationGel Permeation Chromatography - GPC
Isolation and Separation Methods J. Poustka, VŠCHT Praha, ÚAPV 2014, http://web.vscht.cz/poustkaj Gel Permeation Chromatography - GPC Separation and clean-up method Group separation of compounds with similar
More informationLiquid Chromatography
Liquid Chromatography 1. Introduction and Column Packing Material 2. Retention Mechanisms in Liquid Chromatography 3. Method Development 4. Column Preparation 5. General Instrumental aspects 6. Detectors
More informationAnalytical Chemistry
Analytical Chemistry Chromatographic Separations KAM021 2016 Dr. A. Jesorka, 6112, aldo@chalmers.se Introduction to Chromatographic Separations Theory of Separations -Chromatography Terms Summary: Chromatography
More informationDetermination of Caffeine by HPLC
Determination of Caffeine by HPLC Introduction It was a long history before real high performance liquid chromatography (HPLC) had evolved. The very first indication of a chromatographic separation was
More informationChemistry 325 Instrumental Methods of Analysis March 13, Final Exam. Name
Final Exam Name Instructions: This exam is worth 100 points. Some questions allow a choice as to which parts are answered. Only answer the number of parts requested. 1. (32 points) Circle the best answer
More informationParticle Characterization Laboratories, Inc.
Analytical services Particle size analysis Dynamic Light Scattering Static Light Scattering Sedimentation Diffraction Zeta Potential Analysis Single Point Titration Isoelectric point determination Aqueous
More information1. Bulk polymerization of styrene at 60 C with four different initiator concentrations
Learning targets: ideal kinetics of chain growth, I rule, viscometry, characteristics of polymers (number-, weight- and viscosity-average) Assignment of tasks: 1. Bulk polymerization of styrene at 6 C
More informationChromatographic Analysis
Chromatographic Analysis Distribution of Analytes between Phases An analyte is in equilibrium between the two phases [S 1 ] [S 2 ] (in phase 1) (in phase 2) AS [S2 ] K 2 A S [S1 ] 1 AS, A 1 S Activity
More informationDendritic Star Polymer of Polyacrylamide Based on β-cyclodextrin Trimer: A. Flocculant and Drug Vehicle
Electronic Supplementary Material (ESI) for New Journal of Chemistry. This journal is The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2016 Electronic Supporting Information
More informationCHEMISTRY Unit 3, Area of Study 1: Chemical Analysis
Watch this lesson online: https://edrolo.com.au/vce/subjects/chemistry/vce-chemistry/aos-1-chemical-analysis/chromatography-hplc-glc/column-chromatography/#watch CHEMISTRY Unit 3, Area of Study 1: Chemical
More informationHow Molecular Weight and Branching of Polymers Influences Laser Sintering Techniques
How Molecular Weight and Branching of Polymers Influences Laser Sintering Techniques Dr. Bernd Tartsch Malvern Instruments GmbH Rigipsstr. 19, D-71083 Herrenberg Tel: +49-703-97 770, Fax: +49-703-97 854
More informationChemistry Instrumental Analysis Lecture 31. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 31 High Performance Liquid Chromatography (HPLC) High Performance Liquid Chromatography (HPLC) High Performance Liquid Chromatography (HPLC) Solvent Delivery
More informationPrinciples of Gas- Chromatography (GC)
Principles of Gas- Chromatography (GC) Mohammed N. Sabir January 2017 10-Jan-17 1 GC is a chromatographic technique utilizes gas as the mobile phase which is usually an inert gas (Hydrogen, Helium, Nitrogen
More informationCharacterisation of Viscosity and Molecular Weight of Fractionated NR
Characterisation of Viscosity and Molecular Weight of Fractionated NR ENG A.H. Science & Technology Innovation Centre, Ansell Shah Alam, Malaysia (e-mail: engah@ap.ansell.com) Abstract Viscosity of a rubber
More informationClearing the Confusion: GPC, SEC, GFC What, When, Why, and How?
Clearing the Confusion: GPC, SEC, GFC What, When, Why, and How? Jean Lane Applications Engineer LC Columns & Consumables Technical Support January 19, 2017 What we will cover What - Clarification of the
More informationHPLC Praktikum Skript
HPLC Praktikum Skript Assistants: Gianluca Bartolomeo HCI D330, 3 46 68, bartolomeo@org.chem.ethz.ch Sahar Ghiasikhou HCI E330, 2 29 29, ghiasikhou@org.chem.ethz.ch 1. Introduction In chromatographic techniques,
More information