CSH Technology: ACQUITY CSH Columns Applications Notebook

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1 CS Technology: ACQUITY CS Columns Applications otebook

2 List of compounds analyzed using csh columns Lit Code Title Page WA Acetaminophen Impurities WA Acids, Bases, and eutrals WA Aromatic Acids WA Basic Drugs WA Beta Blockers WA Buproprion ydrochloride (Wellbutrin) in Extended Release Tablets 7 WA ozapine Impurities 8 WA Food Additives 9 WA Formic and Acetic Acid 0 WA8 Mirtazapine Impurities WA7 onsteroidal Anti-Inflammatory Drugs (SAIDS) WA9 rganic Acids WA8 Paroxetine Impurities WA9 Paroxetine Isomers WA0 Pharmaceutical Test Mix WA7 Statin Drugs 7 WA Sulfa Drugs 8 WA Tricyclic Antidepressants 9 WA0 Veterinary Drugs 0

3 ACQUITY CS WA Analysis of Acetaminophen Impurities Using ACQUITY CS Columns LC CDITIS ACQUITY CS Fluoro-Phenyl,. x 0 mm,.7 μm Part umber: 800 Mobile Phase A: 0.% acetic acid in Mobile Phase B: acetonitrile Gradien: Time Flow (min) (ml/min) %A %B Curve Initial Initial μl Column Temperature: 0 C Weak eedle Wash: Strong eedle Wash: Filter Time Constant: 9/ /acetonitrile 9/ acetonitrile/ 70 nm 0 points/sec 0. sec ACQUITY UPLC System with -Aminophenol -itrophenol Acetaminophen -Chloroacetanilide Aminophenol ( mg/ml). Acetaminophen (0. mg/ml). -itrophenol ( mg/ml). -Chloroacetanilide ( mg/ml) min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

4 LC CDITIS Columns: ACQUITY CS C 8,. x 0 mm,.7 μm Part umber: 8009 ACQUITY CS Phenyl-exyl,. x 0 mm,.7 μm Part umber: 8000 ACQUITY CS Fluoro-Phenyl,. x 0 mm,.7 μm Part umber: 800 Mobile Phase A: 0.% formic acid in (p ~.7) Mobile Phase B: Gradient Table: acetonitrile Time (min) Flow (ml/min) Analysis of Acids, Bases, and eutrals Using ACQUITY CS Columns %A %B Curve Imipramine S Amitriptyline ACQUITY CS WA Initial 0. 9 Initial Pyrenesulfonic acid Flavone μl (PL) /7 methanol/ C Column Temperature: 0 C Fenoprofen Weak eedle Wash: 9/ /acetonitrile Strong eedle Wash: 9/ acetonitrile/ nm 0 points/sec C Filter Time Constant: 0. sec ACQUITY UPLC System with column manager Diclofenac ctanophenone and detector ACQUITY CS C8 7. Imipramine (00 µg/ml). Amitriptyline (00 µg/ml). -Pyrenesulfonic acid (0 µg/ml) 0. ACQUITY CS Phenyl-exyl Flavone (0 µg/ml). Fenoprofen (00 µg/ml). Diclofenac (00 µg/ml) 7. ctanophenone (00 µg/ml) ACQUITY CS Fluoro-Phenyl min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

5 ACQUITY CS WA Analysis of Aromatic Acids Using ACQUITY CS Columns LC CDITIS ACQUITY CS C 8,. x 0 mm,.7 μm Part umber: 8009 Mobile Phase A: 0.% acetic acid in Mobile Phase B: acetonitrile Time Flow (min) (ml/min) %A %B Curve Sample Concentration: 0 µg/ml each Initial Initial μl Column Temperature: 0 C Weak eedle Wash: Strong eedle Wash: Filter Time Constant: 9/ /acetonitrile 9/ acetonitrile/ 80 nm 0 points/sec 0. sec ACQUITY UPLC System with C Protocatechuic acid C -ydroxybenzoic acid C C Vanillic acid C C C C Syringic acid Salicylic acid,-dihydroxyphenylacetic acid (DPAC) C C,-Dichlorophenoxyacetic acid (Weed B Gon) 0.0. Protocatechuic acid.,-dihydroxyphenylacetic acid (DPAC) 0.. -ydroxybenzoic acid. Vanillic acid. Syringic acid Salicylic acid 7.,-Dichlorophenoxyacetic acid (Weed B Gon) min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

6 LC CDITIS Part umber: 8000 ACQUITY CS Phenyl-exyl,. x 0 mm,.7 μm Mobile Phase A: 0 mm ammonium formate, p Mobile Phase B: acetonitrile μl Column Temperature: 0 C Weak eedle Wash: Strong eedle Wash: Filter Time Constant: Time (min) 9/ /acetonitrile 9/ acetonitrile/ 0 nm 0 points/sec 0. sec ACQUITY UPLC System with ACQUITY UPLC PDA Flow (ml/min) %A %B Curve Initial 0. 9 Initial Analysis of Basic Drugs Using ACQUITY CS Columns C C C -aminopyrazine Quinine Labetalol C Verapamil C C S Pindolol Diltiazem Amitriptyline C ACQUITY CS WA aminopyrazine ( μg/ml). Pindolol (0 μg/ml) 0.. Quinine (0 μg/ml) Quinine impurity. Labetalol (0 μg/ml). Diltiazem impurity 7. Diltiazem ( μg/ml) Verapamil (0 μg/ml) 9. Amitriptyline ( μg/ml) min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

7 ACQUITY CS WA Analysis of Beta Blockers Using ACQUITY CS Columns LC CDITIS ACQUITY CS Phenyl-exyl,. x 0 mm,.7 μm Part umber: 8000 Mobile Phase A: 0 mm ammonium formate, p Mobile Phase B: acetonitrile Time Flow (min) (ml/min) %A %B Curve Initial 0. 9 Initial μl Sample Concentration: 0 µg/ml each Column Temperature: 0 C Weak eedle Wash: Strong eedle Wash: Filter Time Constant: 9/ /acetonitrile 9/ acetonitrile/ 80 nm 0 points/sec 0. sec ACQUITY UPLC System with C Atenolol Metoprolol Pindolol Labetalol xprenolol Propranolol Atenolol. Pindolol. Metoprolol. xprenolol. Labetalol Propranolol min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

8 LC CDITIS Part umber: 8009 Mobile Phase A: Mobile Phase B: Mobile Phase C: Analysis of Bupropion ydrochloride (Wellbutrin) in Extended Release TabletsUsing ACQUITY CS Columns ACQUITY CS C 8,. x 0 mm,.7 μm acetonitrile % formic acid in Time (min) Flow (ml/min) %A %B %C Curve Sample Preparation ProcedurE Ten extended release tablets (each containing 00 mg bupropion hydrochloride) were homogenized in 0 ml of 0/0 methanol/ to give 0 mg/ml of bupropion hydrochloride. The sample was then centrifuged at 000 rpm for 0 minutes. The supernatant was then diluted to 0.0 mg/ml in and filtered through a 0. µm PVDF syringe filter prior to injection. ACQUITY CS WA Initial Initial μl Sample Concentration: 0 µg/ml Bupropion Column Temperature: 0 C Wash Solvent: 0/0 /acetonitrile Purge Solvent: 0/0 /acetonitrile ACQUITY UPLC -ass with Compound. Bupropion (pk a = 7.9) Peak width (.%) =. seconds USP tailing factor = min 7 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

9 ACQUITY CS WA Analaysis of ozapine Impurities Using ACQUITY CS Columns LC CDITIS ACQUITY CS Phenyl-exyl,. x 0 mm,.7 μm Part umber: 8000 Mobile Phase A: Mobile Phase B: acetonitrile Mobile Phase C: % formic acid in Time Flow (min) (ml/min) %A %B %C Curve μl 80/0 methanol/ Sample Concentration: 00 µg/ml (impurities present at ~0.%) Column Temperature: 0 C Wash Solvent: Purge Solvent: Initial Initial /0 /acetonitrile 0/0 /acetonitrile ACQUITY UPLC -ass with and SQD MS Conditions Capillary:.0 kv (ES + ) Cone: Extractor: 0 V V Desolvation Temperature: 0 C Source Temperature: 0 C Desolvation Gas: Scan Range: Scan Rate: 8-Chloro--(piperazin--yl)-dibenzo[b,e][,]-diazepine 800 L/hr 00 to 0 m/z 000 Da/sec ozapine -[-[(-Amino--chlorophenyl)amino] benzoyl]--methylpiperazine,'-(piperazine-,-diyl) bis(8-chloro--dibenzo [b,e][,]diazepine) 8-Chloro-,0-dihydro- -dibenzo[b,e][,] diazepin--one Intensity Intensity Intensity Intensity UV nm - 0 min.0 x 0.0 x x 0.0 x x 0.0 x x 0.0 x 0 Extracted Ion Chromatogram at m/z. Extracted Ion Chromatogram at m/z. Extracted Ion Chromatogram at m/z 9. Extracted Ion Chromatogram at m/z.0. 8-Chloro--(piperazin--yl)--dibenzo[b,e][,]-diazepine. ozapine. -[-[(-Amino--chlorophenyl)amino]benzoyl]- -methylpiperazine.,'-(piperazine-,-diyl)bis(8-chloro--dibenzo[b,e] [,]diazepine). 8-Chloro-,0-dihydro--dibenzo[b,e][,]diazepin--one 0 0 min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation. 8

10 LC CDITIS Part umber: 800 Mobile Phase A: Mobile Phase B: ACQUITY CS Fluoro-Phenyl,. x 0 mm,.7 μm 0.% phosphoric acid in acetonitrile Initial μl Column Temperature: 0 C Weak eedle Wash: Strong eedle Wash: Filter Time Constant: Time (min) 9/ /acetonitrile 9/ acetonitrile/ 0 nm 0 points/sec 0. sec Flow (ml/min) ACQUITY UPLC System with %A %B Curve Analysis of Food Additives Using ACQUITY CS Columns p-ydroxybenzoic acid Dehydroacetic acid S Saccharin Sorbic acid C Methyl paraben ACQUITY CS WA p-ydroxybenzoic acid (0 µg/ml). Sorbic acid (0 µg/ml). Dehydroacetic acid (00 µg/ml). Methyl paraben ( µg/ml). Saccharin (00 µg/ml) min 9 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

11 ACQUITY CS WA Analysis of Formic and Acetic Acids Using ACQUITY CS Columns LC CDITIS ACQUITY CS C 8,. x 00 mm,.7 μm Part umber: Isocratic Mobile Phase: 0.% phosphoric acid in Flow Rate: 0. ml/min μl Sample Concentration: 0.0% Column Temperature: 0 C Weak eedle Wash: 9/ /acetonitrile Strong eedle Wash: 9/ acetonitrile/ 0 nm 0 points/sec Filter Time Constant: 0. sec ACQUITY UPLC System with Formic acid Acetic acid Formic acid. Acetic acid min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation. 0

12 LC CDITIS ACQUITY CS C 8,. x 0 mm,.7 μm Part umber: 8009 Mobile Phase A: 0.% ammonium hydroxide in Mobile Phase B: acetonitrile Time Flow (min) (ml/min) %A %B Curve μl Sample Concentration: mg/ml of mirtazapine USP resolution standard Column Temperature C Weak eedle Wash: Strong eedle Wash: Filter Time Constant: Initial Initial /0 acetonitrile/ 0/0 /acetonitrile 0/0 acetonitrile/ 90 nm 0 points/sec 0. sec ACQUITY UPLC System with Analysis of Mirtazapine Impurities Using ACQUITY CS Columns Mirtazapine Impurity A Mirtazapine Impurity D Mirtazapine Impurity B Mirtazapine Impurity C Mirtazapine Impurity E ACQUITY CS WA8 Mirtazapine Impurity F Mirtazapine Mirtazapine Impurity A (0.%). Mirtazapine Impurity B (0.0%). Mirtazapine Impurity C or F (0.09%). Mirtazapine Impurity D (0.%). Mirtazapine (98.8%). Unknown impurity (0.08%) 7. Mirtazapine Impurity E (0.%) 8. Mirtazapine Impurity C or F (0.%) min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

13 ACQUITY CS WA7 Analysis of onsteroidal Anti-Inflammatory Drugs (SAIDS) Using ACQUITY CS Columns LC CDITIS ACQUITY CS Phenyl-exyl,. x 0 mm,.7 μm Part umber: 8000 Mobile Phase A: Mobile Phase B: acetonitrile Mobile Phase C: % formic acid in Time Flow (min) (ml/min) %A %B %C Curve Tolmetin C Initial Initial Fenoprofen C Ibuprofen μl Column Temperature: 0 C Wash Solvent: Purge Solvent: 0/0 /acetonitrile 0/0 /acetonitrile 70 nm 0 points/sec C aproxen C C Diclofenac Filter Time Constant: 0. sec ACQUITY UPLC -ass System with C C Indomethacin 0.. Tolmetin (0 µg/ml) 0.. aproxen (0 µg/ml). Fenoprofen (0 µg/ml) 0.. Ibuprofen (00 µg/ml) 0.0. Indomethacin (0 µg/ml). Diclofenac (0 µg/ml) min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

14 LC CDITIS ACQUITY CS C 8,. x 0 mm,.7 μm Part umber: Mobile Phase: 0.% phosphoric acid in Isocratic Flow Rate: 0. ml/min to μl Sample Concentration: µg/ml (0 µg/ml for lactic acid) (0 µg/ml for lactic acid) Column Temperature: C Weak eedle Wash: 9/ /acetonitrile Strong eedle Wash: 9/ acetonitrile/ 0 nm 0 points/sec Filter Time Constant: 0. sec ACQUITY UPLC System with Sample Preparation Procedure: Samples were filtered through a 0. μm PTFE syringe filter and injected directly. Analysis of rganic Acids Using ACQUITY CS Columns DL-Tartaric acid Lactic Acid Succinic Acid L-Malic acid Acetic Acid C C C Citric Acid ACQUITY CS WA Vitamin-fortified (grape flavor). DL-Tartaric acid. L-Malic acid. Lactic Acid. Acetic Acid. Succinic Acid. Citric Acid Lemon-lime sports drink Standard mix min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

15 ACQUITY CS WA8 Analysis of Paroxetine Impurities Using ACQUITY CS Columns LC CDITIS ACQUITY CS Fluoro-Phenyl,. x 00 mm,.7 μm Part umber: 800 Mobile Phase A: Mobile Phase B: methanol Mobile Phase C: % acetic acid in Time Flow (min) (ml/min) %A %B %C Curve Pre-Injector volume: 00 µl μl Sample Concentration: paroxetine at 0. mg/ml; impurities at Column Temperature: 0 C Wash Solvent: Purge solvent: Filter Time Constant: Initial Initial mg/ml each 0/0 /acetonitrile 0/0 /acetonitrile 9 nm 0 points/sec 0. sec ACQUITY UPLC -ass System with F Paroxetine trans--phenyl--[[,-(methylenedioxy) phenoxy]methyl]piperidine F F cis-paroxetine (S-trans)--Methyl--[(,-benzodioxol--yloxy) methyl]--(-fluorophenyl)piperidine. trans--phenyl--[(,-methylenedioxy) phenoxymethyl]-piperidine hydrochloride. cis-paroxetine. Paroxetine hydrochloride. (S-trans)--Methyl--[(,-benzodioxol-- yloxy)methyl]--(-fluorophenyl)piperidine min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

16 LC CDITIS Part umber: 800 Mobile Phase A: Mobile Phase B: Mobile Phase C: ACQUITY CS Fluoro-Phenyl,. x 00 mm,.7 μm methanol Pre-Injector volume: 00 µl % acetic acid in μl Sample Concentration: µg/ml each Column Temperature: 0 C Wash Solvent: Purge Solvent: Time (min) Flow (ml/min) 0/0 /acetonitrile 0/0 /acetonitrile ACQUITY UPLC -ass System with SQD %A %B %C Curve Initial Initial Analysis of Paroxetine Isomers Using ACQUITY CS Columns MS Conditions Capillary:.0 kv (ES + ) Cone: 0 V Extractor: V Desolvation Temperature: 0 C Source Temperature: 0 C Desolvation Gas: 800 L/hr Scan Range: 00 to 00 m/z Scan Rate: 000 Da/sec F cis-paroxetine F (+)-trans-paroxetine ACQUITY CS CS Columns WA9 8x0 7x0. cis-paroxetine. (+)-trans-paroxetine x0 x0 Intensity x0 x0 x0 x0 0 0 Extracted ion chromatogram at m/z min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

17 ACQUITY CS WA0 Analysis of Pharmaceutical Test Mix on ACQUITY CS Columns LC CDITIS Columns: ACQUITY CS C 8,. x 0 mm,.7 μm Mobile Phase A: Mobile Phase B: Mobile Phase C: Part umber: 8009 ACQUITY CS Phenyl-exyl,. x 0 mm,.7 μm Part umber: 8000 ACQUITY CS Fluoro-Phenyl,. x 0 mm,.7 μm Part umber: 800 acetonitrile % formic acid in Time (min) μl Flow (ml/min) %A %B %C Curve Initial Initial F Triamcinolone -Amino-7-chloro--oxo- -[]benzopyrano[,-b] pyridine--carbonitrile 8-Bromoguanosine Acetanilide Br ydrocortisone -Aminofluoranthene Column Temperature: 0 C Wash Solvent: Purge Solvent: 0/0 /acetonitrile 0/0 /acetonitrile nm Perylene Filter Time Constant: 0 points/sec 0. sec ACQUITY UPLC -ass System with α-methyl-7αhydroxyprogesterone Br -Bromofluorene aphtho[,-a]pyrene ACQUITY CS C Bromoguanosine (. µg/ml). Acetanilide (.7 µg/ml) ACQUITY CS Phenyl-exyl Triamcinolone (. µg/ml). ydrocortisone (. µg/ml). -Amino-7-chloro--oxo--[]benzopyrano[,-b]pyridine--carbonitrile (. µg/ml). -Aminofluoranthene (. µg/ml) 7. α-methyl-7α-hydroxyprogesterone ( µg/ml) ACQUITY CS Fluoro-Phenyl Bromofluorene ( µg/ml) 9. Perylene (. µg/ml) 0. aphtho[,-a]pyrene (. µg/ml) 0 min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

18 LC CDITIS Part umber: 800 Mobile Phase A: Mobile Phase B: ACQUITY CS Fluoro-Phenyl,. x 0 mm,.7 μm 0.% formic acid in acetonitrile Time (min) Flow (ml/min) %A %B Curve Analysis of Statin Drugs Using ACQUTY CS Columns Structures a+ C ACQUITY CS CS Columns WA7 Initial 0. 9 Initial Pravastatin sodium C μl F Sample Concentration: 0 µg/ml each Column Temperature: 0 C 80/0 methanol/ Atorvastatin Weak eedle Wash: 9/ /acetonitrile Strong eedle Wash: 9/ acetonitrile/ 8 nm 0 points/sec Lovastatin Filter Time Constant: 0. sec ACQUITY UPLC System with Simvastatin 0.. Pravastatin sodium (Pravachol). Atorvastatin (Lipitor) 0.. Lovastatin (Mevacor). Simvastatin (Zocor) min 7 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

19 ACQUITY CS WA Analysis of Sulfa Drugs Using ACQUITY CS Columns LC CDITIS ACQUITY CS Fluoro-Phenyl,. x 00 mm,.7 μm Part umber: 800 Mobile Phase A: 0.% acetic acid in Mobile Phase B: acetonitrile Time Flow (min) (ml/min) %A %B Curve S Sulfanilamide S Sulfathiazole S Initial 0. 9 Initial μl S Sulfamerazine S Sulfamethazine Sample Concentration: 0 µg/ml each Column Temperature: 0 C Weak eedle Wash: 9/ /acetonitrile S S C S S Strong eedle Wash: 9/ acetonitrile/ nm Sulfamethizole Succinylsulfathiazole Filter Time Constant: 0 points/sec 0. sec ACQUITY UPLC System with S S Sulfamethoxazole Sulfasoxazole 0.0. Sulfanilamide. Sulfathiazole 0.. Sulfamerazine. Sulfamethazine Sulfamethizole. Succinylsulfathiazole 7. Sulfamethoxazole 8. Sulfasoxazole min 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation. 8

20 LC CDITIS Part umber: 8009 Mobile Phase A: Mobile Phase B: Mobile Phase C: ACQUITY CS C 8,. x 0 mm,.7 μm acetonitrile % formic acid in μl Sample Concentration: µg/ml each Column Temperature: 0 C Wash Solvent: Purge Solvent: Filter Time Constant: Time (min) 0/0 /acetonitrile 0/0 /acetonitrile nm 0 points/sec 0. sec Flow (ml/min) ACQUITY UPLC -ass System with and SQD Analysis of Tricyclic Antidepressants Using ACQUITY CS Columns %A %B %C Curve Initial Initial MS Conditions Capillary:.0 kv (ES + ) Cone: 0 V Extractor: V Desolvation Temperature: 0 C Source Temperature: 0 C Desolvation Gas: 800 L/hr Scan Range: 00 to 00 m/z Scan Rate: 000 Da/sec Doxepin Desipramine ACQUITY CS CS Columns WA Imipramine ortriptyline Doxepin. Desipramine. Imipramine. ortriptyline Amitriptyline UV min. Amitriptyline. Trimipramine.0x0 7 Intensity.0x0 7.0x0 7 Trimipramine.0x0 7 MS min 9 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation.

21 ACQUITY CS WA0 Analysis of Veterinary Drugs Using ACQUITY CS Columns LC CDITIS Columns: ACQUITY CS C 8,. x 00 mm,.7 μm Part umber: Mobile Phase A: Mobile Phase B: acetonitrile Mobile Phase C:.0% formic acid in (p ~.7) μl 0/90 methanol/ Column Temperature: 0 C Wash: % formic acid in ACQUITY UPLC -ass with TQD (MassLynx v..) TQD Conditions Capillary:.00 kv Extractor:.00 V RF: 0.0 V Source Temperature: 0 C Desolvation Temperature: 0 C Cone Gas Flow: 0 L/r Desolvation Gas Flow: 800 L/r Collision Gas Flow: Time (min) Flow (ml/min) 0.0 ml/min %A %B %C Curve Initial Initial Compound MRM transition (m/z) Cone (V) CID (ev) Ciprofloxacin.00> >. 0 Enrofloxacin 0.0>.8 0.0>.9 Erythromycin 7.8> >7.9 0 xacillin 0.0>0. 0.0>. xytetracycline.0>..0>.7 0 Penicillin-G.7> >7.0 0 Sulfamerazine.8> >. 0 Sulfamethazine 79.00> >. 0.0>0. Tetracycline.0> % F 9 Ciprofloxacin xytetracycline S Sulfamerazine S 7 8 Sulfamethazine min F Enrofloxacin S xacillin. Ciprofloxacin ( µg/ml). Enrofloxacin ( µg/ml). Erythromycin ( µg/ml). xacillin ( µg/ml). xytetracycline ( µg/ml). Penicillin G ( µg/ml) 7. Sulfamerazine ( µg/ml) 8. Sulfamethazine (0. µg/ml) 9. Tetracycline ( µg/ml) C S Penicillin G C Erythromycin C Tetracycline 00 Waters Corporation. Waters, The Science of What s Possible, ACQUITY CS and ACQUITY UPLC are trademarks of Waters Corporation. 0

22 ACQUITY CS TES

23 ACQUITY CS TES

24 ACQUITY CS TES

25 Sales ffices Austria and European Export (Central South Eastern Europe, CIS and Middle East) Australia Belgium Brazil Canada x0 China CIS/Russia / Czech Republic Denmark Finland France Germany ong Kong The etherlands orway Poland Puerto Rico Singapore Spain Sweden 8 00 Switzerland Taiwan United Kingdom All other countries: Waters Corporation U.S.A ungary 0 08 India and India Subcontinent Ireland 8 00 Italy Japan Korea Mexico 7 00 Waters Corporation. Waters, CS, ACQUITY UPLC, ACQUITY CS, MassLynx and The Science of What s Possible are trademarks of Waters Corporation. June E KK-PDF

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