SUPPLEMENTARY INFORMATION

Transcription

1 DOI: /NCHEM.1644 Electrostatically gated membrane permeability in inorganic protocells Mei Li, Rachel L. Harbron, Jonathan V. M. Weaver, Bernard P. Binks, Stephen Mann Supplementary information: Methods Materials. Partially hydrophobic silica nanoparticles with spherical morphology and primary mean diameter of nm were prepared as follows. In brief, hydrophilic silica nanoparticles were silylated to various extents by reaction with dichlorodimethylsilane in the presence of water followed by drying at 300 C for 2 h. The particular particles used here were surface-functionalized with ca. 50% silanol ( O 3 SiOH) and 50% dimethylsilane ( O 2 Si(CH 3 ) 2 ) groups. Partially hydrophobic clay sheets ( nm in width) were prepared from synthetic hectorite that was partially coated with a di-hydrogenated tallow dimethylammonium salt (Laponite, Rockwood Additives Ltd, OML, OG (SR 4232)). Calcein (bis[n,n-bis(carboxymethyl)aminomethyl]fluorescein, C 30 H 26 N 2 O 13, λ ex = 470 nm; λ em = 509 nm), acriflavine hydrochloride (3,6-diamino-10-methylacridinium chloride hydrochloride, Euflavine, C 27 H 28 Cl 4 N 6, λ ex = 416 nm; λ em = 514 nm), glycine, alkaline phosphatase (ALP, 1000U/mg), phosphatase substrate (4-nitrophenyl phosphate (pnpp), disodium salt hexahydrate), anhydrous dodecane, 3-(trimethoxysilyl)propyl methacrylate (TMSPMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), 1-dodedanethiol (DDT), and anhydrous methanol were purchased from Sigma-Aldrich, and used as received. Tetramethoxysilane (TMOS) was purchased from Fluka, UK. Azobis(isobutyronitrile) (AIBN) was purchased from BDH and recrystallized from methanol prior to use. Preparation and characterization of ph-responsive copolymer. TMSPMA, DMAEMA, MAA, branching monomer (EGDMA), dodecanethiol and anhydrous methanol were added to a glass vessel equipped with a stirrer bar in pre-determined molar ratios and degassed by nitrogen purge for 30 mins. The solution was heated to 70 ºC under an inert atmosphere. Polymerization was started by addition of AIBN and the reaction was left stirring for 48 h. After this time monomer conversions in excess of 97 % were typically achieved and dry methanol was removed by evaporation at reduced pressure. No purification steps were required due to almost complete conversion. The resulting materials were stored in dry methanol under nitrogen and then characterized. Molecular weights, molecular weight distributions and Mark-Houwink α-values were measured in THF using a triple detection gel permeation chromatography (TD-GPC) system. The THF system used was a Viscotek TDA-302 TD-GPC equipped with two ViscoGel HHR- N columns and a guard column with a mobile phase of THF and triethylamine (2.5 %) at 35 ºC and a flow rate of 1 ml min -1. Samples for NMR spectroscopy were recorded in CDCl 3 using a Bruker 400 Ultrashield spectrometer operating at 400 MHz. Hydrodynamic copolymer size distributions and zeta potential values were measured by dynamic light scattering (DLS) using a Malvern Zetasizer (Nano series) Nano-ZS equipped with a 633 nm laser. The scattering angle used was 175, and all samples were measured in a disposable polystyrene cuvette. In general, 1 ml of the dilute copolymer NATURE CHEMISTRY 1

2 solution was added to the dispersion unit at ph values of 10, 9, 7, 5, 4, and 2. The volumeaverage diameters and zeta potential values were obtained from at least 3 repeated runs. The pka of the copolymer was determined by monitoring the change in ph of a copolymer solution initially at ph 10 after addition of ml increments of 0.1M hydrochloric acid to a final ph value of 2. The pka was determined from an average of 3 repeat runs. FTIR spectroscopy (Perkin-Elmer Spectrum I; KBr disks) was undertaken on air-dried TMOS-cross-linked colloidosomes or TMOS-cross-linked, copolymer functionalized colloidosomes. The samples were prepared as described above and investigated after transfer into a continuous water phase. Spectra were also recorded on samples of copolymer 1 after air drying from methanol solutions. Imaging studies. Optical and fluorescence microscopy images of the dye-entrapped colloidosomes dispersed in oil or in aqueous solutions at various ph values were recorded using a Leica DMI3000 B manual inverted fluorescence microscope at 10x or 20x magnification. Three band pass filters were used: excitation at nm with an emission cut off at 510 nm (I3), excitation at nm with an emission cut off at 455 nm (D), and excitation at nm with an emission cut off at 580 nm (N2.1). Line intensity profiles on individual colloidosomes were processed from optical micrographs using Gwyddion software. Fluorescence images were collected at different ph values on over fifty individual colloidosomes and no qualitative difference in permeability behaviour was observed in each case. Calibration curves were determined by fluorescence spectroscopy (Jasco FP-6500) measurements on 0.1 mm bulk aqueous solutions of calcein, acriflavine hydrochloride or rhodamine 6G at ph values between 2 and 10. Excitation at 495 nm (calcein), 416 nm (acriflavine hydrochloride) or 526 nm (rhodamine 6G), and excitation and emission band widths of 3 nm and 1 nm were used, respectively. GPC methods. Molecular weight, polydispersity, and Mark-Houwink α-values (MH-α) were determined using triple-detection (TD-) gel permeation chromatography (GPC). The mobile phase was tetrahydrofuran (THF). The copolymer was well solvated under these conditions. There was no evidence of column adsorption. MH-α were similar for the copolymer as judged in THF GPC runs. Polydispersity values indicated a distribution of molecular weights, consistent with free radical polymerisation that provides minimal true control over composition. The M w was higher than expected. Performing GPC measurements on a copolymer with this level of architectural and compositional complexity remains challenging. Although TD-GPC with the added support of multiple detectors is a valuable tool for polymer characterization, its reliable application to branched copolymer particles is yet to be proven. (J. V. M. Weaver, D. J. Adams, Soft Matter, 2012, 6, ) NATURE CHEMISTRY 2

3 Supplementary Information: Figures Figure S1: NMR spectroscopy and GPC results for the hybrid inorganic-organic branched ph-responsive copolymer, TMSPMA 23 -DMAEMA 40 -MAA 37 -EGDMA 8 -DDT 10. NATURE CHEMISTRY 3

4 Figure S2 (a) Zeta potential, (b) ph titration and (c) DLS data for copolymer, TMSPMA 23 - DMAEMA 40 -MAA 37 -EGDMA 8 -DDT 10 ([copolymer] = 4.790x10-4 mol L -1 in (a) and (c); sample volume = 600 µl in (b). NATURE CHEMISTRY 4

5 Figure S3. Optical image of individual water-filled cross-linked colloidosome prepared in the absence of a copolymer shell and dispersed in dodecane. Note the roughened surface texture. Scale bar = 200 µm polymer SiO2+TMOS SiO2+TMOS+polymer Wavenumber/cm -1 Figure S4 FTIR spectra for polymer 1 (black), TMOS cross-linked colloidosomes (blue) or TMOS cross-linked, copolymer functionalized colloidosomes (red). The colloidosome samples were transferred into water and then air-dried before the spectra were recorded. NATURE CHEMISTRY 5

6 calcein acriflavine hydrochloride rhodamine 6G Figure S5. Molecular structures of dyes (pk a values for calcein: carboxylic acids pka 1 = 2.1, pka 2 = 2.9, pka 3 = 4.2, pka 4 = 5.5; amino groups, pka 5 = 10.8, pka 6 = 11.7; rhodamine 6G pk a = 7.5). NATURE CHEMISTRY 6

7 ph2 ph10 Figure S6 (Top row), fluorescence microscopy images of individual acriflavine hydrochloride-containing TMOS-cross-linked colloidosomes dispersed in oil (left), or water at ph 2 (middle) and 10 (right) showing retention or ph-independent release of the dye in oil and water, respectively. (Bottom row), fluorescence microscopy images of individual rhodamine 6G-containing TMOS-cross-linked colloidosomes dispersed in oil (left), or water at ph 2 (middle) and 10 (right) showing retention or ph-independent release of the dye in oil and water, respectively. Scale bars = 50 µm. NATURE CHEMISTRY 7

8 ph = a Figure S? at end?. Calibration curves in bulk aqueous solution b c ph Figure S7. Fluorescence microscopy images of colloidosomes dispersed in oil and comprising (a,b) entrapped aqueous calcein at various ph values after 2 hours (a) and after 1 day (b), or (c) entrapped acriflavine hydrochloride. In each case, high levels of fluorescence intensity are associated with the colloidosomes. NATURE CHEMISTRY 8

9 Fluorescence inensity (a.u.) (a) calcein Fluorescence inensity (a.u.) (b) acriflavine hydrochloride ph ph (c) rhodamine 6G 30 Fluorescence inensity (a.u.) ph Figure S8 Plots of fluorescence intensity against ph for aqueous solutions of (a) calcein, (b) acriflavine HCl or (c) rhodamine 6G at a concentration of 0.1 mm in each case. The dye concentration was the same as that encapsulated inside the colloidosomes. The error bars were within the standard deviation. ph Figure S9. Fluorescence images of individual rhodamine 6G-containing cross-linked copolymer-functionalized colloidosomes dispersed in water at ph 2, 7 or 9 showing progressive leaching of the entrapped dye with increasing ph. NATURE CHEMISTRY 9

10 Figure S10. Optical images of sample tubes showing retention of coloration in the colloidosome phase when suspended in oil or water at phs between 2 and 10, indicating that proteins such as ferritin, myoglobin or cytochrome c remain encapsulated within the colloidosome interior. NATURE CHEMISTRY 10

11 ph4 2.0 C t - C 0 / µm Model Equation Exponential y = y0 + A*exp(R0*x) Reduced Chi Sqr Adj. R-Squar Value Standard Err B y B A B R Time / h Figure S11. Release profile between hr for calcein-containing cross-linked copolymerfunctionalized colloidosomes at ph 4. a b Figure S12. Uptake properties: Optical fluorescence images of (a) water-filled cross-linked copolymer functionalized colloidosomes or (b) water-filled cross-linked colloidosomes immersed in aqueous solutions of calcein at ph values of 10. Note the absence of dye uptake in the presence of the copolymer. Scale bars = 200 µm. NATURE CHEMISTRY 11

12 DOI: /NCHEM.1644 Figure S13: Plots showing time-dependent changes in 4-nitrophenolate concentrations for ALP-mediated pnpp dephosphorylation in bulk aqueous solution containing copolymermodified silica nanoparticles at ph 7.5 (black), 8.4 (red) and 9.8 (blue). a b Figure S14. Ferritin-containing TMOS cross-linked, copolymer-functionalized colloidosomes after heating at 80 C for 4 hours in oil (a) and in water (b). NATURE CHEMISTRY

13 Figure S15: UV-vis spectra and calibration plots for calcein at various ph values in bulk solution. NATURE CHEMISTRY 13

14 Figure S16: UV-vis spectra and calibration plots for rhodamine 6G at various ph values. The Uv-vis spectra of Rh6G were almost identical at all ph values. NATURE CHEMISTRY 14