INTRODUCTION OVERVIEW
|
|
- Annabella Russell
- 5 years ago
- Views:
Transcription
1 COMPARISON OF CCS(N 2 ) MEASUREMENTS OBTAINED FROM TWO DIFFERENT T-WAVE ION S WITH DIRECT MEASUREMENTS USING A DRIFT TUBE ION Kevin Giles, Martin Palmer, Keith Richardson, Nick Tomczyk Waters MS Technologies Centre, Wilmslow, UK OVERVIEW Favourable comparison of two alternative geometry T-Wave ion mobility systems (IMS QTof and Q IMS Tof) with a drift tube ion mobility system and literature values Best correlation between measured CCS(N 2 ) values from drift tube and calibrated T-Wave IM devices achieved when CCS calibrant and analyte charge state are matched INTRODUCTION With recent developments in ion mobility (IM) separation instrumentation, interest has increased in the determination of collision cross-sections (CCS) of various classes of compounds. These CCS measurements can be used to augment screening of complex samples both by reducing interfering effects of matrix ions and as an additional identification criterion 1. Additionally they assist in structural confirmation of isoforms of protein complexes 2 and structural isomers of small molecules. The widely used T-Wave IM system relies on calibration to provide CCS values as there is no direct analytical solution for the complex motion of ions through the device. The efficacy of T- wave CCS calibration is investigated by comparison of CCS values obtained from a linear-field drift tube IM system, theoretical calculations and available literature values. TO DOWNLOAD A COPY OF THIS POSTER NOTE, VISIT 1
2 METHODS Instrumentation All CCS data were measured in nitrogen drift gas (CCS(N 2 )). The instruments used for this study were a SYNAPT G2-Si, a modified SYNAPT G2 -Si where the T-wave IM cell was replaced with a linear field drift tube and a novel geometry T-Wave IM instrument, Vion IMS QTof, a schematic of which is shown in Figure 1. Figure 1. Schematic of Vion IMS QTof. SYNAPT G2-Si and Vion IMS QTof All data were acquired chromatographically with an ACQUITY I-Class and electrospray ionisation. A simple linear gradient of acidified water and acetonitrile was used to separate analytes of interest. The T-wave IM systems were calibrated using a mixture of acetaminophen and poly-d,l-alanine (Sigma- Aldrich) using the automated calibration routine in the acquisition control software. The Vion IMS QTof calibration also corrects for mass dependant transmission time post IM separation. IM T-wave parameters: SYNAPT G2-Si Vion IMS QTof velocity ramp 1000 to 350ms -1 at 40V velocity ramp 850 to 350ms -1 at 60V Linear field drift tube (DT) SYNAPT G2-Si All CCS data were acquired by direct infusion at 3μL/min into the electrospray source. Each sample was analysed at three different N 2 IM gas pressures (circa 1.8, 2.0 and 2.4 mbar). At each gas pressure the drift time was measured at eight individual linear fields to remove the constant offset from the drift time and the CCS was calculated using the Mason-Schamp equation. The CCS value is reported as the mean obtained from the three gas pressures. Typical linear fields were between 2 and 12 Vcm -1 with a drift tube length of 25.5cm. Theoretical Calculation of CCS(N 2 ) Values In order to obtain energy minimised structures, an initial structural and protomer search was carried out. This was followed by a full density functional theory structural optimisation and charge distribution calculation 3. CCS(N 2 ) values were calculated using a nitrogen optimised version of MOBCAL 4. ION S WITH DIRECT MEASUREMENTS USING A DRIFT TUBE ION 2
3 Table 1. List of compound classes analysed. All samples for chromatographic analysis were prepared in mobile phase A (0.1% aqueous formic acid), samples for direct infusion were prepared in 1:1 water:acetonitrile + 0.1% formic acid. RESULTS Comparison of T-Wave systems and Linear Field Drift Tube The data obtained from the DT SYNAPT G2-Si compares favourably with those values available in the literature 5,6, see Figure 2. Representative CCS(N 2 ) values for a selection of drug-like small molecules are shown in Table 2. Good correlation can be observed in Table 2 between the two calibrated T-Wave systems and the linear field drift tube. This can also be observed in Figure 3 for all compounds. Figure 3. Differences between DT SYNAPT G2- Si measured CCS(N 2 ) values and those obtained on Vion IMS QTof and a standard SYNAPT G2-Si. Furthermore when data from the two T-Wave IM systems and the DT SYNAPT G2-Si are compared to the literature values good correlation is observed, see Figure 4. This further demonstrates that the CCS(N 2 ) values determined by a calibrated T-Wave system (of either geometry) are equivalent to those obtained by a linear field drift tube IM system. Table 2. CCS(N 2 ) values for selected drug-like small molecules. Figure 4. Absolute (upper trace) and difference (lower trace) of measured CCS(N 2 ) values from DT SYNAPT G2-Si, standard SYNAPT G2-Si, Vion IMS QTof and those obtained by Bush and McLean. Figure 2. Differences between DT SYNAPT G2-Si measured CCS(N 2 ) values and those obtained by Bush and McLean. As demonstrated above, good CCS(N 2 ) reproducibility can be achieved across multiple IM MS system platforms, however, it should be noted that within all of these values there will be a small degree of experimental variance for all techniques and as such no one value is necessarily correct and as such, terms like error and accuracy should be used with caution when comparing experimentally derived CCS values. ION S WITH DIRECT MEASUREMENTS USING A DRIFT TUBE ION 3
4 Effect of Charge State on Calibrated CCS(N 2 ) Measurements It has been demonstrated by Dodds et al 7 that on a calibrated T-Wave IM system to achieve the most comparable CCS(N 2 ) values with those obtained with a linear field drift tube system the charge state of the calibrant and analyte should ideally be matched. This effect can be observed in Figure 5. This shows the measured CCS(N 2 ) values (from Vion IMS QTof) for a series of a singly and doubly charged ions when a calibration created from a singly charged series (blue trace) or doubly charged series (red trace) is applied to the acquired data. The T-Wave IM CCS(N 2 ) values for those components whose charge state matches the charge state of the calibrant exhibit the smallest deviation with respect to the linear field drift tube measured CCS(N 2 ) values. Matching charge is advised to obtain the best absolute CCS (N 2 ) values, however for targeted screening purposes, the calibration method just needs to be consistent between method development and analysis. In order to prevent this pressure effect occurring in Vion IMS QTof a real time feedback loop has been implemented where the trap and IM pressure are continuously measured and any changes in the pressure are compensated for by adjusting the nitrogen flow rate delivered by the trap and IM mass flow controllers. Once active the real time pressure monitor corrects for changes in source pressure conditions every 100ms. This phenomenon can be observed in Figure 6, where measured drift time values of a small molecule mix are compared. The sample mix was infused into a t-piece along with either 250μL/ min 8/2 acetonitrile/water or 800μL/min 1/9 acetonitrile/water delivered by an LC system to mimic a significant change in LC gradient and hence source pressure conditions. A shift in the measured drift time is observed when the post solvent composition is changed and the dynamic pressure control (DPC) is disabled (blue in Figure 6) whereas no shift is observed when the DPC is enabled (pink in Figure 6). Figure 5. Effect of calibration charge state on a mix of singly and doubly charged ions Measured CCS(N 2 ) Reproducibility of Vion IMS QTof system In order to maintain drift time (and hence CCS) reproducibility it is important to have constant IM cell parameters, such as fields, pressures etc.. Any external factors affecting these conditions may have a detrimental effect on experimental precision. An aspect of the geometry of an IM QTof is that any changes in pressure in the source region of the instrument (e.g. due to different LC flow rates or composition) will cause a corresponding change in the trap and IM pressure. This change in pressure will manifest itself as a change in overall drift time and hence measured CCS(N 2 ). This can also result in different measured CCS(N 2 ) values if data are compared between an infusion and chromatographic acquisition or using when different LC conditions to those used during calibration or generation of library CCS(N 2 ) values. These effects are not observed on the SYNAPT platform (geometry Q IM Tof) owing to the high pressure ion mobility cell is isolated from the source by a high vacuum quadrupole region. Figure 6. Dynamic pressure control minimising drift time shift for changes in chromatographic conditions. A further benefit of dynamic pressure control is illustrated in Figures 7 and 8, which demonstrate long term stability of measured CCS(N2) values of 400 replicate injections of a drug-like small molecule mix. A high degree of precision is obtained over the 33 hours taken to acquire these data, which would not be achieved without the dynamic pressure control preventing pressure drift. The measured CCS(N 2 ) %RSD are summarised in Table 3. ION S WITH DIRECT MEASUREMENTS USING A DRIFT TUBE ION 4
5 CONCLUSION Figure 7. Measured CCS(N 2 ) reproducibility on Vion IMS QTof of various drug-like small molecules. Table 3. CCS(N 2 ) reproducibility on Vion IMS QTof for 400 replicate injections. Figure 8 shows the % deviation from the mean for each analyte. Excellent correlation has been observed between in house and literature drift tube CCS(N 2 ) values. It has been demonstrated that the CCS (N 2 ) values determined by calibrated T- Wave systems (of either geometry) are equivalent to those obtained by a linear field drift tube IM systems. The term deviation should be used to describe observed variation between experimentally measured CCS(N 2 ) values not error or accuracy as these terms imply that a given experimentally observed value is correct and free from measurement error with respect to another experimentally observed value. The charge state of a calibration should ideally be matched to intended analytes, however for targeted screening purposes, the calibration method just needs to be consistent between method development and analysis. A novel dynamic IM pressure adjustment results in improved robustness of observed CCS(N 2 ) values. Good correlation observed between theoretically derived and experimentally determined CCS(N 2 ) values. Comparison of Measured and Theoretically Derived CCS(N 2 ) Values Excellent correlation has been observed for experimentally measured and theoretically derived CCS (N 2 ) values 3, see Table 4 for acetaminophen. References 1. Goscinny S. et al, 61st ASMS Conference, Minneapolis, MN, 9th-13th June Robinson C et al, Annu. Rev. Phys. Chem. 2015, 66, Paizs B, Bangor University, personal communication 4. Campuzano I et al, Anal. Chem. 2012, 84, McLean J et al, Anal. Chem. 2014, 86, Bush M, ccsdatabase/ 7. Dodds, E. et al Anal. Chem. 2014, 86, Table 4. Experimental and theoretical CCS(N 2 ) values for acetaminophen Waters Corporation 5
The Use of the ACQUITY QDa Detector for a Selective, Sensitive, and Robust Quantitative Method for a Potential Genotoxic Impurity
The Use of the ACQUITY QDa Detector for a Selective, Sensitive, and Robust Quantitative Method for a Potential Genotoxic Impurity Janet Hammond Waters Corporation, Wilmslow, UK APPLICATION BENEFITS High
More informationYun W. Alelyunas, Mark D. Wrona, Russell J. Mortishire-Smith, Nick Tomczyk, and Paul D. Rainville Waters Corporation, Milford, MA, USA INTRODUCTION
Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma Yun W. Alelyunas, Mark
More informationTraditional Herbal Medicine Structural Elucidation using SYNAPT HDMS
Traditional Herbal Medicine Structural Elucidation using SYNAPT HDMS with Time-Aligned Parallel (TAP) Fragmentation Kate Yu, Jose Castro-Perez, and John Shockcor Waters Corporation, Milford, MA, U.S. INTRODUCTION
More informationQuantitation of High Resolution MS Data Using UNIFI: Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays
: Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays Mark Wrona, Jayne Kirk, and Yun Alelyunas Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Ability
More information[ instrument specifications ]
Designed for leading researchers working at the limits of conventional mass spectrometry capabilities who need to further characterize and define their samples the Waters SYNAPT High Definition MS (HDMS
More informationAnalysis of Labeled and Non-Labeled Proteomic Data Using Progenesis QI for Proteomics
Analysis of Labeled and Non-Labeled Proteomic Data Using Progenesis QI for Proteomics Lee Gethings, Gushinder Atwal, Martin Palmer, Chris Hughes, Hans Vissers, and James Langridge Waters Corporation, Wilmslow,
More informationHigh-Throughput Protein Quantitation Using Multiple Reaction Monitoring
High-Throughput Protein Quantitation Using Multiple Reaction Monitoring Application Note Authors Ning Tang, Christine Miller, Joe Roark, Norton Kitagawa and Keith Waddell Agilent Technologies, Inc. Santa
More informationLive Webinar : How to be more Successful with your ACQUITY QDa Detector?
Live Webinar : How to be more Successful with your ACQUITY QDa Detector? Q&A Transcript ---------------- Q - How do you generate multiple charges reproductively? A - If you use the same settings on the
More informationAnalytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS
Application Note Clinical Research Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Authors Yanan Yang 1, Victor Mandragon 2, and Peter Stone 1 1
More information[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS
Building a Collision Cross Section Library of Pharmaceutical Drugs Using the Vion IMS QTof Platform: Verification of System Performance, Precision and Deviation of CCS Measurements Yun Wang Alelyunas,
More informationAB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching Scores by Removal of Isobaric Interferences
AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching s by Removal of Isobaric Interferences Differential Mobility Used as a Tool to Address Selectivity Challenges Adrian M. Taylor
More informationIdentification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry
Identification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry Jonathan Williams Waters Corporation, Milford, MA, USA A P P L I C AT
More informationA Workflow Approach for the Identification and Structural Elucidation of Impurities of Quetiapine Hemifumarate Drug Substance
A Workflow Approach for the Identification and Structural Elucidation of Impurities of Quetiapine Hemifumarate Drug Substance Michael D. Jones, Marian Twohig, Karen Haas, and Robert S. Plumb Waters Corporation,
More informationTHE IMPLEMENTATION OF A SCREENING WORKFLOW FOR ION MOBILITY QUADRUOPOLE TIME-OF-FLIGHT MASS SPECTROMETRIC ANALYSIS OF PFOS ISOMERS
THE IMPLEMENTATION O A SCREENING WORKLOW OR ION MOBILITY QUADRUOPOLE TIME-O-LIGHT MASS SPECTROMETRIC ANALYSIS O POS ISOMERS Ken Rosnack ken_rosnack@waters.com Ingrid Ericson Jogsten and Bert van Bavel
More informationFast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma
Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Jon Bardsley 1, James Goldberg 2 1 Thermo Fisher Scientific, Runcorn, UK; 2 Thermo Fisher Scientific, West Palm Beach,
More informationAchieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer
APPLICATION NOTE 72385 Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer Authors Stephan Meding, Katherine Lovejoy, Martin Ruehl Thermo Fisher Scientific,
More informationEstimating Collision Cross Sections from Travelling Wave IM-MS Data: How it is done and what are the Problems?
Estimating Collision Cross Sections from Travelling Wave IM-MS Data: How it is done and what are the Problems? Johanna Hofmann and Kevin Pagel Fritz Haber Institute of the Max Planck Society, Department
More informationExtraction of Methylmalonic Acid from Serum Using ISOLUTE. SAX Prior to LC-MS/MS Analysis
Application Note AN89.V.1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Page 1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Prior to LC-MS/MS Analysis Sample Preparation
More informationRapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS
Rapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS Application Note Forensic Toxicology Authors Martin Josefsson, and Markus Roman National Board of Forensic Medicine Linköping,
More informationSimplified Approaches to Impurity Identification using Accurate Mass UPLC/MS
Simplified Approaches to Impurity Identification using Accurate Mass UPLC/MS Marian Twohig, Michael D. Jones, Dominic Moore, Peter Lee, and Robert Plumb Waters Corporation, Milford, MA, USA APPLICATION
More informationDesigned for Accuracy. Innovation with Integrity. High resolution quantitative proteomics LC-MS
Designed for Accuracy High resolution quantitative proteomics Innovation with Integrity LC-MS Setting New Standards in Accuracy The development of mass spectrometry based proteomics approaches has dramatically
More informationBioanalytical Chem: 4590: LC-MSMS of analgesics LC-MS Experiment Liquid Chromatography Mass Spectrometry (LC/MS)
Liquid Chromatography Mass Spectrometry (LC/MS) Prelab Questions: Questions to be answered before doing the experiment. The answers are due at the beginning of each experiment without exception (the questions
More informationOverview. Introduction. André Schreiber AB SCIEX Concord, Ontario (Canada)
Quantitation and Identification of Pharmaceuticals and Personal Care Products (PPCP) in Environmental Samples using Advanced TripleTOF MS/MS Technology André Schreiber AB SCIEX Concord, Ontario (Canada)
More informationIon Mobility Mass Spectrometry of Complex Carbohydrates Collision Cross Sections of Sodiated N- linked Glycans. - Supporting Information -
Ion Mobility Mass Spectrometry of Complex Carbohydrates Collision Cross Sections of Sodiated N- linked Glycans Kevin Pagel, 1 *, David J. Harvey 2, 3 - Supporting Information - 1 Fritz Haber Institute
More informationApplying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis
PO-CON1768E Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide ASMS 2017 TP-194 David Baker 1, Christopher Titman 1, Neil Loftus 1, Jonathan Horner
More informationAssay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer
38 Current Trends in Mass Spectrometry November 6 Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer This article demonstrates the improved
More informationQuantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 1260 Infinity Binary LC system with UV detection
Quantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 126 Infinity Binary LC system with UV detection Application Note Food Author Srividya Kailasam Agilent Technologies,
More informationToxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative. Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish.
1 Supporting Information 2 3 Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish. 4 5 John Moreman, Okhyun
More informationIdentification and Characterization of an Isolated Impurity Fraction: Analysis of an Unknown Degradant Found in Quetiapine Fumarate
Identification and Characterization of an Isolated Impurity Fraction: Analysis of an Unknown Degradant Found in Quetiapine Fumarate Michael D. Jones, Xiang Jin Song, Robert S. Plumb, Peter J. Lee, and
More informationProven robustness for large-scale metabolomics studies using the Phenomics Workhorse
Proven robustness for large-scale metabolomics studies using the Phenomics Workhorse HRAM LC-MS profiling of > 1 urine samples demonstrates the outstanding analytical skills of the impact II Introduction
More informationUse of High Speed/High Resolution Size-Based Chromatographic Separation of Polymeric Mixtures with Offline Infrared Detection
Use of High Speed/High Resolution Size-Based Chromatographic Separation of Polymeric Mixtures with Michael O Leary 1, Jennifer Gough 1, Damian Morrison 1, Alain Creissen 2 1 Waters Corporation, Milford,
More informationOVERVIEW INTRODUCTION. Michael O Leary, Jennifer Gough, Tanya Tollifson Waters Corporation, Milford, MA USA
Use of High Speed/High Resolution Size-Based Chromatographic Separation of Surfactants and Oligomeric Materials with Single Quadrupole Mass Spectrometry Michael O Leary, Jennifer Gough, Tanya Tollifson
More informationConformational Characterization of Calmodulin by Hydrogen Deuterium Exchange Mass Spectrometry
Conformational Characterization of Calmodulin by Hydrogen Deuterium Exchange Mass Spectrometry Joomi Ahn, Martha Stapels, Michael Eggertson, Keith Fadgen, and Ying Qing Yu Waters Corporation, Milford,
More informationMaximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System
Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Application Note Authors Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon, and
More informationRoutine MS Detection for USP Chromatographic Methods
Daniel S. Root, Thomas E. Wheat, and Patricia McConville Waters Corporation, Milford, MA, USA APPLICATION BENEFITS This approach enables mass spectral analysis of peaks directly from unmodified USP HPLC
More informationAnalysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research
Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Heather A Brown, 1 Claudia Rossi, 2 and Lisa J Calton 1 1 Waters Corporation, Wilmslow, UK 2
More information- Supporting Information -
Protomers of Benzocaine: Solvent and Permittivity Dependence Stephan Warnke, Jongcheol Seo, Jasper Boschmans, Frank Sobott, James H. Scrivens, Christian Bleiholder,,? Michael T. Bowers, Sandy Gewinner,
More informationMultiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap Orbitrap Hybrid Mass Spectrometer
Application ote: 54 Multiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap rbitrap Hybrid Mass Spectrometer Kate Comstock, Yingying Huang; Thermo Fisher Scientific,
More informationFORENSIC TOXICOLOGY SCREENING APPLICATION SOLUTION
FORENSIC TOXICOLOGY SCREENING APPLICATION SOLUTION A purpose-built collection of the best-inclass components for forensic toxicology Whether you re challenged to present reliable and secure forensic sample
More informationIon-mobility mass spectrometry of polyoxometalate Keplerate clusters and their supramolecular assemblies
SUPPLEMENTARY INFORMATION Table of Contents Summary 1. General materials and methods 2. High-resolution mass spectra 3. Ion-mobility mass spectra 4. Fragmentation of clusters in the TWIMS drift tube 5.
More informationAnalysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF
application Note Liquid Chromatography/ Mass Spectrometry Author Sharanya Reddy PerkinElmer, Inc. Shelton, CT USA Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF
More informationA Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies
A Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies APPLICATION BENEFITS Regulatory guidelines and recommendations place a greater emphasis on the detection
More informationDEMONSTRATING SUPERIOR LINEARITY: THE ACQUITY UPLC PHOTODIODE ARRAY DETECTOR
DEMONSTRATING SUPERIOR LINEARITY: THE ACQUITY UPLC PHOTODIODE ARRAY DETECTOR Tanya Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION ACQUITY UPLC PDA LINEARITY There are two characteristics that
More informationTHE IMPLEMENTATION OF A SCREENING WORKFLOW FOR ION MOBILITY QUADRUOPOLE TIME-OF-FLIGHT MASS SPECTROMETRIC ANALYSIS OF PFOS ISOMERS
THE IMPLEMENTATION OF A SCREENING WORKFLOW FOR ION MOBILITY QUADRUOPOLE TIME-OF-FLIGHT MASS SPECTROMETRIC ANALYSIS OF PFOS ISOMERS Ericson Jogsten I 1 *, Mullin L 2, Dillon L 3, Burgess J 2, McCullagh
More informationMulti-stage Mass Spectrometry up to MS 4 on a QTof System
Valerie Koppen, 1 Russell Mortishire-Smith, 2 Filip Cuyckens 1 1 Janssen R&D, Beerse, Belgium 2 Waters Corporation, Wilmslow, United Kingdom APPLICATION BENEFITS This application note illustrates how multi-stage
More informationPosterREPRINT COMPARISON OF PEAK PARKING VERSUS AUTOMATED FRACTION ANALYSIS OF A COMPLEX PROTEIN MIXTURE. Introduction
Introduction The study of protein expression allows a greater understanding of biological function and can now routinely be performed using mass spectrometry. However, analysis of the post-translational
More informationFinnigan LCQ Advantage MAX
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Finnigan LCQ Advantage MAX The Finnigan LCQ Advantage MAX ion trap mass spectrometer
More informationQuantitative Analysis and Identification of Migrants in Food Packaging Using LC-MS/MS
Quantitative Analysis and Identification of Migrants in Food Packaging Using LC-MS/MS Cécile Busset 1 and Stephen J. Lock 2 1 AB SCIEX, Paris (France); 2 AB SCIEX, Warrington, Cheshire (U.K.) Introduction
More informationLC-MS/MS in the Clinical Laboratory. Jo Adaway
LC-MS/MS in the Clinical Laboratory Jo Adaway LC-MS/MS in the clinical laboratory LC-MS/MS is mainly used for quantification of analytes used in the diagnosis and monitoring of disease Semi-quantitative
More informationImproved Ion Mobility Separation of Protein Conformations in the Gas Phase with SYNAPT G2 HDMS
Improved Ion Mobility Separation of Protein Conformations in the Gas Phase with SYNAPT G2 HDMS St John Skilton, Iain Campuzano, Kevin Giles, Hilary Major, and Weibin Chen Waters Corporation, Milford, MA,
More informationAnalysis of Low-Calorie Sweeteners by Liquid Chromatography-Tandem Mass Spectrometry
Analysis of Low-Calorie Sweeteners by Liquid Chromatography-Tandem Mass Spectrometry Application Note Food safety Authors Ismael Flores and Carlos Sepulveda Agrolab México Km 7 Carretera Pachuca-Actopan
More informationAgilent 385-ELSD Evaporative Light Scattering Detector
Agilent 8-ELSD Evaporative Light Scattering Detector For Low Molecular Weight Compounds Data Sheet Introduction Evaporative light scattering detectors are ideal for detecting analytes with no UV chromophore
More informationFast and Flexible Optimization of Modifier Concentrations Using an Agilent 1290 Infinity LC System with Blend Assist
Fast and Flexible Optimization of Modifier Concentrations Using an Agilent 129 Infinity LC System with Technical Overview Author A.G.Huesgen Agilent Technologies, Inc. Waldbronn, Germany Abstract During
More informationElectron Transfer Dissociation of N-linked Glycopeptides from a Recombinant mab Using SYNAPT G2-S HDMS
Electron Transfer Dissociation of N-linked Glycopeptides from a Recombinant mab Using SYNAPT G2-S HDMS Jonathan P. Williams, Jeffery M. Brown, Stephane Houel, Ying Qing Yu, and Weibin Chen Waters Corporation,
More informationThermo Finnigan LTQ. Specifications
IET International Equipment Trading Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Thermo Finnigan LTQ Specifications
More informationTomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories
APPLICATION NOTE 65200 Tomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories Authors Neloni Wijeratne, Claudia Martins, Mary
More informationFereshteh Zandkarimi, Samanthi Wickramasekara, Jeff Morre, Jan F. Stevens and Claudia S. Maier
Chapter 2 Electrospray Ionization Traveling Wave Ion Mobility Spectrometry Mass Spectrometry for the Analysis of Plant Phenolics: An Approach for Separation of Regioisomers Fereshteh Zandkarimi, Samanthi
More informationGUIDELINES FOR THE DESIGN OF CHROMATOGRAPHIC ANALYTICAL METHODS INTENDED FOR CIPAC COLLABORATIVE STUDY
Page 1 of 13 CIPAC/4105/R GUIDELINES FOR THE DESIGN OF CHROMATOGRAPHIC ANALYTICAL METHODS INTENDED FOR CIPAC COLLABORATIVE STUDY Prepared for CIPAC by Dr M J Tandy*, P M Clarke and B White (UK) The rapid
More informationFUTURE CONFIRMATORY CRITERIA
FUTURE CONFIRMATORY CRITERIA Revision CD 2002/657 Marco Blokland, Bjorn Berendsen, Hans Mol, Leen van Ginkel, Saskia Sterk REVISION 2 Revision 2002/657 EU Commission has explicitly asked for a revision
More informationLC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode
LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode Application Note Authors Siji Joseph Agilent Technologies India Pvt. Ltd.
More informationClinical Toxicology. Biomass Component Extraction: The uneaten cooked plant specimen was prepared for
Clinical Toxicology Page of 0 Materials and Methods Biomass Component Extraction: The uneaten cooked plant specimen was prepared for chemical analysis as follows. The sample was frozen, diced, pulverized
More informationAccurate Mass Analysis of Hydraulic Fracturing Waters: Identification of Polyethylene Glycol Surfactants by LC/Q-TOF-MS
Accurate Mass Analysis of Hydraulic Fracturing Waters: Identification of Polyethylene Glycol Surfactants by LC/Q-TOF-MS Application Note Authors E. Michael Thurman and Imma Ferrer Center for Environmental
More informationConfirmation of In Vitro Nefazodone Metabolites using the Superior Fragmentation of the QTRAP 5500 LC/MS/MS System
Confirmation of In Vitro Nefazodone Metabolites using the Superior Fragmentation of the QTRAP 5500 LC/MS/MS System Claire Bramwell-German, Elliott Jones and Daniel Lebre AB SCIEX, Foster City, California
More informationA Case of Pesticide Poisoning: The Use of a Broad-Scope Tof Screening Approach in Wildlife Protection
A Case of Pesticide Poisoning: The Use of a Broad-Scope Tof Screening Approach in Wildlife Protection George Keenan 1, Anna Giela 1, Michael Taylor 1, Jennifer Watson 1, Eleanor Riches 2, James Morphet
More informationLirui Qiao, 1 Rob Lewis, 2 Alex Hooper, 2 James Morphet, 2 Xiaojie Tan, 1 Kate Yu 3
Using Natural Products Application Solution with UNIFI for the Comparison of the Chemical Ingredients of Shuanghuanglian Oral Drink from Two Different Manufacturers Lirui Qiao, 1 Rob Lewis, 2 Alex Hooper,
More informationHigh-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes
High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes Joe DiBussolo, Marta Kozak Thermo Fisher Scientific, San Jose, CA Application
More informationUSP Method Transfer and Routine Use Analysis of Irbesartan Tablets from HPLC to UPLC
USP Method Transfer and Routine Use Analysis of Tablets from HPLC to UPLC Aparna Chavali, Tanya Jenkins and Patricia McConville Waters Corporation, 34 Maple Street, Milford, MA USA APPLICATION BENEFITS
More informationImproved Screening for 250 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer
Improved Screening for 2 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer Mary lackburn, Jia Wang, Jonathan eck, Charles Yang, Dipankar Ghosh, Thermo Fisher Scientific, San Jose, C,
More informationAgilent 1200 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity
Agilent 12 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity A case study with degraded omeprazole drug product Application Note Small Molecule
More informationApplication Note. Edgar Naegele. Abstract
Fast identification of main drug metabolites by quadrupole time-of-flight LC/MS Measuring accurate MS and MS/MS data with the Agilent 651 Q-TOF LC/MS and identification of main meta-bolites by comparison
More informationVOC measurements in ambient air using Selected Ion Flow Tube Mass Spectrometry-automation and calibration considerations
VOC measurements in ambient air using Selected Ion Flow Tube Mass Spectrometry-automation and calibration considerations Environmental Chemistry group, water Science Forum and the Separation Science Group
More informationAgilent All Ions MS/MS
Agilent All Ions MS/MS Workflow Overview A Determine fragment ions for LC/MS Quant method B Develop final Quant method Develop LC/MS Qualitative Analysis method Process data with Find by Formula Build
More informationQuadrupole Time-of-Flight Liquid Chromatograph Mass Spectrometer LCMS-9030 C146-E365
Quadrupole Time-of-Flight Liquid Chromatograph Mass Spectrometer LCMS-9030 C146-E365 Effortless Performance The LCMS-9030 quadrupole time-of-flight (Q-TOF) mass spectrometer integrates the world s fastest
More informationSEAMLESS INTEGRATION OF MASS DETECTION INTO THE UV CHROMATOGRAPHIC WORKFLOW
SEAMLESS INTEGRATION OF MASS DETECTION INTO THE UV CHROMATOGRAPHIC WORKFLOW Paula Hong, John Van Antwerp, and Patricia McConville Waters Corporation, Milford, MA, USA Historically UV detection has been
More informationTargetScreener. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS
TargetScreener A Comprehensive Screening Solution for Forensic Toxicology Innovation with Integrity UHR-TOF MS TargetScreener Get the Complete Picture Forensic laboratories are frequently required to perform
More informationLC-HRMS: Challenges for Routine Implementation
Joint EURL pesticides meeting October 23 rd -25th, 2013 Almeria Spain LC-HRMS: Challenges for Routine Implementation Richard J. Fussell Food and Environment Research Agency (York, UK) www.fera.defra.gov.uk
More informationAgilent 385-ELSD Evaporative Light Scattering Detector
Agilent 8-ELSD Evaporative Light Scattering Detector For Low Molecular Weight Compounds Data Sheet Introduction Evaporative light scattering detectors are ideal for detecting analytes with no UV chromophore
More informationAnalyst Software. Automatic Optimization Tutorial
This document is provided to customers who have purchased AB Sciex equipment to use in the operation of such AB Sciex equipment. This document is copyright protected and any reproduction of this document
More informationSupporting information Online HPLC-ESI-HRMS Method for the Analysis and Comparison of Different Dissolved Organic Matter Samples
1 2 3 4 5 6 Supporting information Online HPLC-ESI-HRMS Method for the Analysis and Comparison of Different Dissolved Organic Matter Samples Claudia Patriarca a, *, Jonas Bergquist a, Per J. R. Sjöberg
More informationQuality control analytical methods- Switch from HPLC to UPLC
Quality control analytical methods- Switch from HPLC to UPLC Dr. Y. Padmavathi M.pharm,Ph.D. Outline of Talk Analytical techniques in QC Introduction to HPLC UPLC - Principles - Advantages of UPLC - Considerations
More informationAPPLICATION SOLUTIONS FOR STRUCTURAL PROTEOMICS
APPLICATION SOLUTIONS FOR STRUCTURAL PROTEOMICS TABLE OF CONTENTS The Application of SYNAPT High Defintion Mass Spectrometry for the Conformational Studies of Protein Complexes... 1 High Definition Mass
More informationHplc Lc Ms And Gc Method Development And Validation Guideline For Academic And Industrial Scientists Involved In Method Development And Validation
Hplc Lc Ms And Gc Method Development And Validation Guideline For Academic And Industrial Scientists Involved We have made it easy for you to find a PDF Ebooks without any digging. And by having access
More informationVALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION
VALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION Andrew J. Aubin and Tanya L. Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION Benzocaine (4-Aminobenzoic
More informationNatural Product Application Solution with UNIFI (NPAS)
Natural Product Application Solution with UNIFI (NPAS) Application Presentation Warren B Potts III Senior Director Americas Marketing warren_potts@waters.com 2017 Waters Corporation 1 Diverse World of
More informationLC-MS Based Metabolomics
LC-MS Based Metabolomics Analysing the METABOLOME 1. Metabolite Extraction 2. Metabolite detection (with or without separation) 3. Data analysis Metabolite Detection GC-MS: Naturally volatile or made volatile
More informationHplc Lc Ms And Gc Method Development And Validation Guideline For Academic And Industrial Scientists Involved In Method Development And Validation
Hplc Lc Ms And Gc Method Development And Validation Guideline For Academic And Industrial Scientists Involved We have made it easy for you to find a PDF Ebooks without any digging. And by having access
More informationProudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment LCQ Deca XP Plus
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment LCQ Deca XP Plus Improved Ion Optics for Greater Sensitivity and Precision
More information4.1 MATERIALS 4.2 EQUIPMENTS
4.1 MATERIALS 1. Aceclofenac Moraceae Pharma Ltd., Uttarakhand, India 2. Diclofenac Arti Drugs Ltd., Mumbai, India 3. Paracetamol Moraceae Pharma Ltd., Uttarakhand, India 4. Para-aminophenol Hema Pharmaceuticals
More informationEvaluation of a New HPLC, a New Tandem MS and a New Data Processing Software for General Clinical Use
Evaluation of a New HPLC, a New Tandem MS and a New Data Processing Software for General Clinical Use Shih-Tse Jason Lai, Jia Wang, Brad Hart, Kristine Van Natta, Marta Kozak, Jorge Valdivia, Haibo Wang,
More informationTypes of Analyzers: Quadrupole: mass filter -part1
16 Types of Analyzers: Sector or double focusing: magnetic and electric Time-of-flight (TOF) Quadrupole (mass filter) Linear ion trap Quadrupole Ion Trap (3D trap) FTICR fourier transform ion cyclotron
More informationApplication Note. Abstract. Authors. Environmental
Determination of Acidic Herbicides using an Agilent 6460 Triple Quadrupole LC/MS Equipped with Agilent Jet Stream Technology and Direct Aqueous Injection, for Potable and Environmental Samples Application
More informationThe Analysis of Residual Solvents in Pharmaceutical Products Using GC-VUV and Static Headspace
The Analysis of Residual Solvents in Pharmaceutical Products Using GC-VUV and Static Headspace The Analysis of Residual Solvents in Pharmaceutical Products Using GC-VUV and Static Headspace_Rev3 Introducing
More informationOnline Reaction Monitoring of In-Process Manufacturing Samples by UPLC
Online Reaction Monitoring of In- Manufacturing Samples by UPLC Tanya Tollifson Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Better throughput, yield, and process understanding are possible
More informationOptimization of the sample preparation and extraction methodology
Supporting information Results and discussion Optimization of the sample preparation and extraction methodology Visual comparison of the slopes (curve in standard mixture vs MMC curve with identical concentration
More informationMulti-residue analysis of pesticides by GC-HRMS
An Executive Summary Multi-residue analysis of pesticides by GC-HRMS Dr. Hans Mol is senior scientist at RIKILT- Wageningen UR Introduction Regulatory authorities throughout the world set and enforce strict
More informationPlasma-free Metanephrines Quantitation with Automated Online Sample Preparation and a Liquid Chromatography-Tandem Mass Spectrometry Method
Plasma-free Metanephrines Quantitation with Automated Online Sample Preparation and a Liquid Chromatography-Tandem Mass Spectrometry Method Xiang He and Marta Kozak ThermoFisher Scientific, San Jose, CA,
More informationSupporting Information
1 Supporting Information 2 3 Discovery and implications of C 2 and C 3 perfluoroalkyl sulfonates in aqueous film forming foams (AFFF) and groundwater 4 Krista A. Barzen-Hanson a and Jennifer A. Field b*
More informationLC-MS/MS Method for the Determination of Diclofenac in Human Plasma
LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core
More informationEPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS
Application Note EPA Method 535 EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS API 3200 LC/MS/MS System Overview Described here is the
More informationVarian 385-LC. ELSD for Low Molecular Weight Compounds
D ATA S H E E T Varian 8-LC EVAPORATIVE LIGHT SCATTERING DETECTOR ELSD for Low Molecular Weight Compounds Evaporative light scattering detectors are ideal for detecting analytes with no UV chromophore
More information