Bulk Silica Gels. SiliaFlash Irregular Silica Gels SiliaSphere PC Spherical Silica Gels

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1 SiliaFlash Irregular Silica Gels SiliaSphere PC Spherical Silica Gels

2 4 Chromatography at SiliCycle SiliCycle is your partner of choice for your purification and chromatography needs. Recognized as a leader with an outstanding quality silica gel, SiliCycle offers one of the largest selection on the market. They are available in two different shapes: SiliaFlash Irregular silicas SiliaSphere PC Spherical silicas Get unbeatable performance with our products. SiliCycle: Silica Expert With pore diameters ranging from 30 to 1,000 Ångström (Å) and particle sizes up to 1,000 microns (µm), SiliCycle offers products to meet all your requirements. We offer one of the most reliable portfolios for flash and gravity grades for low to medium-high pressure. Our silica gels are ideal for preparative chromatography, from laboratory to pilot-plant processes and production scale. Features and Benefits of SiliaFlash & SiliaSphere PC Features High purity silica gels Lowest level of fines on the market Exceptional narrow particle and pore size distributions Batch-to-batch, year-to-year consistency Neutral ph Low metal content & controlled water content High mechanical stability High surface area and density Availability in bulk quantities Benefits No contamination, consistency, reliability, reproducibility No contamination, lower back-pressure, superior separation Optimal separation and resolution Reliable chromatography Wide range of products can be purified, even acid sensitive ones Symmetrical peaks without tailing Can be used under high pressures without surface abrasion Greater loading capacity, enabling more silica for the same volume Solvent economy (smaller dead volume) Always in stock with on-time delivery Together, all these benefits mean optimal and reproducible separation power, saving you time and money. SiliCycle, the Silica Supplier for Every Need Each year, SiliCycle manufactures hundreds of tons of silica for a broad range of chromatography applications. All our products are manufactured under tightly controlled manufacturing processes and a stringent quality control ensures the highest quality. Be confident in scaling-up your processes with our silica gels. With SiliCycle, No Scale-up Limitations Scaling-up from laboratory to production scale

3 5 Two Shapes Available From SiliCycle: Irregular & Spherical The quality of a silica gel is extremely important when you are using it for chromatography purposes, particularly when dealing with difficult separations of valuable compounds. You need to be confident about your recoveries. In chromatography, there are at least three physical properties that will influence your separation and that you need to consider when choosing your silica gel: Particle shape (irregular or spherical) Particle size distribution (tight or large) Pore diameter (surface area) These caracteristics will directly influence crucial parameters involved in a successful chromatography: Resolution (efficiency of separation & final purity) Retention (which allows separation) Capacity (maximal sample quantity and final recovery / yield) Back-pressure (speed and pumps related issues) At SiliCycle, we ensure consistency, reliability & reproducibility. Our expertise and strong knowledge has been developed over many years of helping our customers find the best solutions to their particular needs. How to Choose Between SiliaFlash Irregular or SiliaSphere PC Spherical Gels? Irregular silica gels are traditional in flash or gravity chromatography and have always been a spontaneous choice for preparative chromatography. Nowadays, spherical particles are now used increasingly. Cost is very important in preparative and process chromatography, and the use of monodisperse spherical particles with narrow particle size distribution is more expensive. It is possible in this case to use irregular silica but the separation may not provide the desired results. For these situations, SiliCycle has developed a more affordable class of spherical particles for preparative chromatography: SiliaSphere PC. The advantage of using SiliaSphere PC materials over standard silica gels includes the following: Increased efficiency of the eluent s flow characteristics Higher resolution Ease of packing / better packing reproducibility Higher mechanical stability SiliaSphere PC: Truly Spherical Silica gel quality varies greatly between manufacturers. Even when advertised as being spherical this may not be the case. Please discover on next page a quick comparison of Scanning Electron Microscopy (SEM) pictures between SiliCycle SiliaSphere PC and the competition. TEL.: FAX: TOLL-FREE: SILICYCLE (NORTH AMERICA ONLY) INFO@SILICYCLE.COM

4 6 SiliaFlash & SiliaSphere PC Exceptional Characteristics Tight Particle and Pore Size Distributions The importance of the particle and pore size distributions varies depending on the type of chromatography being done. For instance, it is very important when using HPLC that the particle size distribution of the spherical particles being used remain very narrow. Importance of Tight Distributions in Chromatography Tight Particle Size Distribution Greater column performance and separation Tighter peaks and better peak shape Tight Pore Size Distribution Surface area (Presence of bigger pore size leads to lower surface availability) Optimal peak shape (Presence of smaller pore size leads to peak tailing) Better column packing, easier to pack No preferential pathways (channeling) Faster flow rate with lower back-pressure No molecule sequestration due to fluid diffusion inside pores Time and solvent savings Scanning Electron Microscopy (SEM) comparison of two IRREGULAR silica gels µm, 60 Å Scanning Electron Microscopy (SEM) comparison of two SPHERICAL silica gels 50 µm, 60 Å SiliCycle Competitor SiliCycle Competitor Effects of Homogeneous vs Uneven Packing The connection between particle size distribution and column performance is very simple. When the distribution is broad, the packing is uneven. Some parts are composed of only large particles where the solvent will flow fast and meet little resistance, and there are sections composed of small particles where the solvent flows slowly and meets great resistance. As a result, the solvent will take the path of least resistance through the column and flow around the pockets of small particles instead of straight through the column. This uneven flow greatly affects the separation because the compounds will have different retention times depending on their flow path. As they exit the column, the compounds will give broad and poorly separated peaks. The figure on the right illustrates the effect of a wide particle size distribution versus a narrow one. Narrower distribution gives a more homogenous packing and thus more concentrated fractions. And, by reducing solvent consumption, the process will be more cost-efficient. Preferential path chosen Wide Particle Size Distribution Narrow Particle Size Distribution SiliaFlash SiliaSphere PC Path straight through the column

5 7 SiliaFlash, one of the Tightest Particle Size Distribution on the Market Of particle size distributions disparity When selecting a silica gel, chemists need to take into account that not all μm gels are the same. In this example, the figure on the right shows the distribution curves of SiliCycle s SiliaFlash gel (PN: R10030B) compared to other manufacturers of flash silica gels of same particle sizes. All products were sold as μm 60 Å gels. As you can observe, SiliCycle s gel has a mean of 90 % of the particles in the nominal range compared to maximum 80 % for the competitor gels. The higher the curve, the tighter the particle size distribution. Of the importance of the absence of fines Volume (%) Particle size (µm) In chromatography, fine particles (small particles under 10 microns) increase back-pressure and can result in clogging, which is particularly dangerous when using glass columns. Fines can also pass through filters and contaminate final products. The lack of fines gives a more regular, stable and reproducible chromatography bed and a faster and more even flow rate for better separation. The zoomed part of the figure shows that our most popular silica gel, SiliaFlash microns 60 Å, has total absence of fines unlike the six competitor gels analyzed. Volume (%) SiliaFlash µm 10 A B C 5 D E F Particle size (µm) SiliCycle has the lowest level of fines on the market for both SiliaFlash & SiliaSphere PC. Particle Size Analysis Methods Laser Diffraction (Malvern Analysis) Typically used for particle sizes below 40 microns. Particle size distribution is reported in term of D10, D50 (average, mean) and D90. Some manufacturers also mention the ratio of D90/D10. Sieving Usually for particle sizes over 40 microns. Particle size distribution is reported in percentage of undersized and oversized. TEL.: FAX: TOLL-FREE: SILICYCLE (NORTH AMERICA ONLY) INFO@SILICYCLE.COM

6 8 High Purity Silica Gels You can be sure of the outstanding quality of SiliCycle s silica gels because of the closely controlled manufacturing conditions. Our tight control of every manufacturing process step allows reproducible results (chemical, physical and structural) as well as ensuring the same chromatographic selectivity. Hence, SiliaFlash and SiliaSphere PC are suitable for validated chromatographic processes. Our stringent Quality Control and Quality Assurance ensures high performance with no scale-up limitations. Every product meets our quality specifications and is shipped with a Certificate of Analysis (CofA). Individual data sheets are also available directly from our website. Every day, SiliCycle s silica gels are being used by thousands of satisfied scientists for their purifications. They know that SiliaFlash and SiliaSphere PC are synonymous of quality and that they can expect reproducible results every time. Stable Water Level Content Water level of silica gel affects the selectivity of the silica. SiliaFlash and SiliaSphere PC have generally a water content between 2 to 6 %. This is advantageous for you since other products have a water variation from 2 to 15 % depending on the manufacturer. SiliCycle can also adjust the water level upon request. Neutral ph Our silicas are ph-adjusted between 6 and 8 to be safely used in the separation of a wide range of products (a neutral ph is needed to separate ph-sensitive compounds). Once again, this is advantageous when compared to many gels on the market that are much more acidic. Low Trace Metal Content Silica, depending on its method of manufacturing, contains a certain amount of various metals. This can, in turn, affect the quality of the separation. Aluminum, iron and lead are particularly problematic because they cause peak tailing. SiliCycle s proprietary technology generates a silica gel with the lowest trace metal content on the market. This ensures you will get optimal performance from your chromatography. Tight control of metals in every batch also improves your reproducibility and reduces risks of interaction between metals and desired compounds. Typical Metal Content Comparison for µm, 60 Å Silica Gels Metals SiliCycle F60 R10030B Manufacturer A Manufacturer B Metal (mg/kg) Aluminum Al Barium Ba Calcium Ca 336 1, Iron Fe Magnesium Mg Sodium Na Titanium Ti Zirconium Zr

7 9 SiliaFlash Irregular Silica Gels Two Different Grades for Different Needs Over the years, SiliCycle has developed two different grades for the two most popular irregular gels used in the industry: 1) μm, 60 Å 2) μm, 60 Å Those two grades of each gel are available to address all our customers requirements, depending on their applications, areas of research, budgets and so on μm, 60 Å Gels: SiliaFlash F60 (R10030B) VS SiliaFlash P60 (R12030B) Both compare favorably with the overall industry average of a μm 16 distribution, and each grade offers its own particle size distribution profile. SiliaFlash F60 R10030B % size up to grade Two Different Grades of μm, 60 Å Gels 12 SiliaFlash P60 R12030B Characteristics F60 (R10030B) P60 (R12030B) 75 % size up to grade 10 Particle Size (μm) Pore Diameter (Å) Extra step to reduce metal content to minimum level Particularities Tighter particle size distribution Fines have been removed Lower price Fines have been removed Particle Size (µm) The figure on the right shows F60 tighter particle size distribution and the absence of fines for both gels. Volume (%) Acid washed silica gel for extra purity (R10530B) SiliCycle also manufactures an acid washed SiliaFlash μm, 60 Å Silica Gel. SiliCycle s acid washed gel has been developed to ensure a ph-controlled media with even lower levels of trace metal contaminants for maximal purity. Please refer to the table next page for metal content details μm, 60 Å Gels: SiliaFlash G60 (R10040B) VS SiliaFlash GE60 (R10140B) Each grade offers its own particle size distribution profile. Two Different Grades of μm, 60 Å Gels Characteristics G60 (R10040B) GE60 (R10140B) Particle Size (μm) Pore Diameter (Å) Particularities Extra step to reduce metal content to minimum level Tighter particle size distribution Fines have been reduced to minimal level Fines have been reduced to minimal level Lower price The figure on the right shows G60 tighter particle size distribution. TEL.: FAX: TOLL-FREE: SILICYCLE (NORTH AMERICA ONLY) INFO@SILICYCLE.COM Volume (%) SiliaFlash G60 R10040B 90 % size up to grade SiliaFlash GE60 R10140B 80 % size up to grade Particle Size (µm)

8 10 Typical metal content comparison between SiliCycle s five most popular gels Typical Metal Content of Most Popular Irregular Silicas Product Number F60 (R10030B) P60 (R12030B) Acid Washed (R10530B) G60 (R10040B) GE60 (R10140B) Particle Size µm Pore Diameter Å Metal (mg/kg) Aluminum Al < 200 < 1,000 < 70 < 350 < 900 Antimony Sb < 0.2 < 0.2 Arsenic Ar < 1 < 1 Barium Ba < 40 < 40 < 5 < 40 Beryllium Be < 0.1 < 0.1 Bismuth Bi < 1 < 1 Cadmium Cd < 0.01 < 0.01 Calcium Ca < 200 < 500 < 10 < 250 < 500 Chromium Cr < 1 < 1 Cobalt Co < 0.1 < 0.1 Copper Cu < 1 < 1 Iron Fe < 75 < 350 < 10 < 75 < 350 Lead Pb < 1 < 1 Lithium Li < 0.1 < 0.1 Magnesium Mg < 150 < 250 < 10 < 100 < 150 Manganese Mn < 1 < 2 < 1 < 1 Molybdenum Mo < 0.1 < 0.1 Nickel Ni < 1 < 1 Potassium K < 500 < 30 < 2 < 750 < 30 Rubidium Rb < 0.2 < 0.2 Selenium Se < 1 < 1 Silver Ag < 0.1 < 0.1 Sodium Na < 150 < 1,500 < 15 < 150 < 1,500 Strontium Sr < 4 < 15 < 1 < 4 < 15 Tellurium Te < 0.1 < 0.1 Thallium Tl < 0.1 < 0.1 Tin Sn < 0.4 < 0.4 < 0.2 < 0.4 Titanium Ti < 200 < 250 < 90 < 250 Uranium U < 0.1 < 0.1 Vanadium V < 1 < 1 Zinc Zn < 1 < 1

9 11 Silica Selection Guide Selecting the most appropriate sorbent for any given application can be difficult. To help you choose the right pore diameter and particle size, simply follow the two pathways to select the most suitable sorbent. Selecting Pore Diameter Sample Molecular Weight (MW in Dalton) Organic Molecules Peptides Proteins (Polypeptides) Bio-polymers MW < 1,000 Dalton 1,000 < MW < 10,000 Dalton MW > 10,000 Dalton 30, 40 Å (< 400 Dalton) 60, 70 Å (400-1,000 Dalton) 90, 100 Å 120, 150, 300 Å 500, 800 Å 1,000 Å Selecting Particle Size Generated Pressure (GP) or Selectivity (α) GP ~950 psi (65 bar) α < 1.2 (very difficult separation) GP ~220 psi (15 bar) 1.2 < α < 1.5 (difficult separation) GP ~73 psi (5 bar) 1.5 < α < 2.0 (possible separation) GP ~29 psi (2 bar) α > 2.0 (easy separation) From 0 to 30 µm From 15 to 40 µm From 40 to 100 µm From 40 to 1,000 µm Selectivity (α) is measured by the retention factor ratio between two similar compounds: α = Tr 1 - T 0 Tr 2 - T 0 TEL.: FAX: TOLL-FREE: SILICYCLE (NORTH AMERICA ONLY) INFO@SILICYCLE.COM

10 12 A Particle Size for Each Application Most Popular Particle Size Applications Particle Size Distribution Irregular Particles Spherical Particles Applications Particles for Preparative TLC Plates From 0 to 20 µm - Contains neither binder (organic or inorganic) nor UV indicator (F 254 ) Can also be used in flash chromatography if higher resolution is required (higher back-pressure) Particles for Difficult Separations From 10 to 45 µm From 15 to 45 µm High-resolution silica for difficult separations (similar polarities) Particles for Flash Chromatography µm From 40 to 75 µm Chromatography types: high-resolution flash chromatography & low to medium-pressure preparative chromatography Narrow particle size distribution Easier to pack and more uniform packing Superior resolution Suitable for use with complex matrices MOST POPULAR PARTICLE SIZE DISTRIBUTION µm From 60 to 150 µm Alternative to µm silica for faster flow rate with lower pressure Particles for Column (or Gravity) Chromatography From 60 to 200 µm From 75 to 250 µm From 120 to 200 µm From 100 to 200 µm Most economical silica for open column chromatography (gravity) Suitable for very dirty purification Easier to handle Silica for standard open column chromatography Narrow particle size distribution enables uniform packing Suitable for mass overload purification Other Application From 200 to 1,000 µm From 200 to 500 µm Silica for plugs

11 13 SiliaFlash & SiliaSphere PC Ordering Information This is only an overview of gels we can provide. Please contact us if you are looking for a different product. Product Number SiliaFlash Ordering Information (µm) Particle Size (mesh) Pore Diameter (Å) R10037L R10130A R10150A R10140A R10160A R10170A R10180A 500-1, R10110B 0-20 * R10019B * R10017B R10023B * R10030B (F60) R12030B (P60) R10530B (acid washed) R10150B R10040B (G60) R10140B (GE60) R10137B R10057B R10160B R10160B R10160B R10170B R10180B 500-1, R10130D R10140D R10157D R10170D R10180D 500-1, R10181D 800-1, R10130H R10150H R10140H R10157H R10160H R10170H R10072H R10180H 500-1, R10181H 800-1, R10130M R10140M R10170M Product Number SiliaSphere PC Ordering Information Formats: 1 kg, 5 kg, 10 kg, 25 kg, etc. Up to multi-ton scale. * Mesh equivalent too small to exist as real screen size. TEL.: FAX: TOLL-FREE: SILICYCLE (NORTH AMERICA ONLY) INFO@SILICYCLE.COM (µm) Particle Size (mesh) S10030B-A S10027B-A S10034B-A S10040B-A S10063B-A S10066B-A S10068B-A S10020C S10040C S10030C S10070C Pore Diameter (Å) S10095D-A S10009E-A 20 - S10020E S10030E S10040E S10065E S10070E S10009G-A 20 * S10030G-A S10034G-A S10040G-A S10063G-A S10020M * S10030M S10040M S10064M S10070M S10020P * S10030P S10040P S10070P S10020S * S10030S S10040S S10070S S10020T * S10030T S10040T S10070T ,000

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