Determination of Hormones in Serum by LC/MS/MS Using Agilent Bond Elut Plexa SPE

Size: px
Start display at page:

Download "Determination of Hormones in Serum by LC/MS/MS Using Agilent Bond Elut Plexa SPE"

Transcription

1 Determination of ormones in Serum by L/MS/MS Using Agilent Bond Elut Plexa SPE Application Note Bio-analysis, Pharmaceutical, linical Research Authors Megan Juck and William Long Agilent Technologies, Inc. Abstract A method for the determination of 1 hormones and their respective internal standards in serum was developed. ormones were extracted from serum using Agilent Bond Elut Plexa Solid Phase Extraction (SPE) ( mg, 1 ml) cartridges. They were separated on an Agilent InfinityLab Poroshell P- column (.1 mm,. µm), and analyzed by liquid chromatography tandem mass spectrometry (L/MS/MS). ormones were analyzed using both positive and negative electrospray ionization (ESI) using a 1 mm ammonium fluoride mobile phase to improve hormone responses in both modes. verall recoveries ranged from to 1%, with relative standard deviations (RSDs) between. and.%. This application note demonstrates the ease-of-use and effectiveness of Bond Elut Plexa SPE cartridges for hormone determination in serum. Introduction A method using polymeric solid phase extraction (SPE) was developed for the determination of steroid hormones in serum. Table 1 shows the structures and chemical formulas for the 1 analyzed hormones. Agilent Bond Elut Plexa has a unique polymeric architecture with a hydroxylated, nonretentive, amide-free surface. Proteins and lipids bind minimally to the polymeric surface, resulting in cleaner samples and reduced matrix interferences. The nonpolar PS-DVB core is ideal for retaining small molecules such as hormones. This application note demonstrates the effectiveness and ease-of-use of Bond Elut Plexa SPE cartridges.

2 Table 1. Molecular formulas and structures for 1 analyzed hormones. Isobaric pair information is also included. ormone Molecular formula Structure ormone Molecular formula Structure Aldosterone 1 -Deoxycorticosterone 1 ortisol 1 Androstenedione 1 ortisone 1 Estrone 1 orticosterone 1 1α Progesterone 1 -Deoxycortisol 1 Dihydrotestosterone (DT) 1 β-estradiol 1 Progesterone 1 Testosterone 1 Isobaric pairs: Aldosterone and cortisone -Deoxycorticosterone and 1α progesterone -Deoxycortisol and corticosterone

3 Experimental Reagents and chemicals All reagents were PL grade or higher. Methanol was bought from oneywell (Muskegon, MI, USA.) Water was purified using an EMD Millipore Milli-Q Integral System (Darmstadt, Germany.) Reagent-grade formic acid (FA, p/n G-) was from Agilent Technologies. Ammonium fluoride was from Sigma-Aldrich (St. Louis, M, USA). -Deoxycorticosterone d was from Toronto Research hemicals (Toronto, ntario, anada). All other hormones and internal standards were from Sigma-Aldrich. Serum (D Mass Spect Gold, MSG) was bought from Golden West Biologicals, Inc. (Temecula, A, USA). Serum was stored at until day of use. Equipment and materials Eppendorf pipettes and repeater oncentration evaporation system Vortexer and multitube vortexers (VWR, Radnor, PA, USA) Agilent Vac Elut SPS manifold with collection rack for 1 1 mm test tubes (p/n 1) Agilent Bond Elut Plexa, mg, 1 ml straight barrel cartridges (p/n 1) Agilent MS analyzed amber screw-top glass vials with write-on spot (p/n 1-1) Agilent bonded screw cap, PTFE/red silicone septa (p/n 1 ) Agilent vial insert, µl, deactivated glass with polymer feet (p/n ) Sample preparation A hormone standard mix and hormone internal standard mix were each prepared in methanol. Table lists the concentrations for each analyte and its respective internal standard. Each internal standard was kept at the same concentration as its respective analyte. For example, aldosterone was at a concentration of ng/ml in the hormone standard mix, and aldosterone-d was ng/ml in the hormone internal standard mix. Table. ormone and internal standard concentrations are provided. Internal standards were spiked at the same concentration as parent analytes. Analyte/internal standard Aldosterone/aldosterone d ortisol/cortisol d ortisone/cortisone d orticosterone/corticosterone d -Deoxycortisol/-deoxycortisol d β-estradiol/β-estradiol d Testosterone/testosterone d -Deoxycorticosterone/-deoxycorticosterone d Androstenedione/androstenedione 1 Estrone/estrone d 1α Progesterone/1α progesterone d DT/DT d Progesterone/progesterone d Standard concentration, each (ng/ml) Instrumentation Analysis was performed on an Agilent 1 Infinity L system consisting of: Agilent 1 Infinity binary pump (GA) Agilent 1 Infinity high performance autosampler (GA) equipped with an Agilent 1 F/ALS thermostat (G1B) Agilent 1 Infinity thermostatted column compartment (G) The L system was coupled to an Agilent A triple quadrupole L/MS/MS system equipped with Agilent Jet Stream Electrospray ionization technology. Agilent Massunter workstation software was used for all data acquisition and analysis.

4 Sample pretreatment Prior to the SPE protocol, µl of.% formic acid (FA) in water was added to 1 µl of serum. Samples were spiked with µl of hormone standard mix and µl of hormone internal standard mix. The final concentration was 1 ng/ml for β-estradiol, β-estradiol d, estrone, estrone d, DT, and DT d. All other hormones and internal standards were at a final concentration of 1 ng/ml in serum. Samples were vortexed and centrifuged to remove any serum particulates. After SPE, the collected eluent was evaporated to dryness under nitrogen at, then reconstituted with 1 µl of / Me/water. Sample was vortexed, then transferred to a vial with a glass insert for L/MS/MS analysis. SPE Protocol ondition: 1 ml Me +.% FA Equilibrate: 1 ml water +.% FA Load: Pretreated serum sample Wash: 1 ml % Me in water Dry: minutes (minimum 1 inch g) Instrument conditions Parameter PL olumn: Guard: Mobile phase: Flow rate: Value olumn temperature: Autosampler temperature: Injection volume: 1 µl Needle wash: Agilent InfinityLab Poroshell P-,.1 mm mm,. µm (p/n ) Agilent InfinityLab Poroshell P-, UPL guard column,.1 mm mm,. µm (p/n 1 ) A) 1 mm ammonium fluoride in water B) acetonitrile (AN). ml/min Gradient: Time (min) % B Stop time: Post-time: MS Electrospray Ionization (ESI) 1:1:1:1 AN/Me/isopropanol/water with. % FA 1 minutes 1 minute Gas temperature: Gas flow: Nebulizer: L/min psi Sheath gas heater: Sheath gas flow: apillary: Nozzle voltage: Delta EMV (+): Delta EMV ( ): L/min Positive, V; Negative, V Positive V; Negative 1, V V V Elute*:. ml Me *Gravity elution was performed. After the eluent flow stopped, a low vacuum (< inch g, - seconds) was applied to fully collect all eluent. Figure 1. SPE protocol for hormone determination using an Agilent Bond Elut Plexa mg, 1 ml cartridge.

5 Table. L/MS/MS dmrm parameters and retention times for target analytes. Analyte RT (min) Precursor ion (m/z) Frag (V) Quant ion (m/z) Product ion E (V) Qual ion (m/z) Aldosterone Positive ortisol Positive ortisone Positive orticosterone Positive -Deoxycortisol Positive E (V) Ionization mode β-estradiol Negative Testosterone Positive -Deoxycorticosterone Positive Androstenedione Positive Estrone Negative 1α Progesterone Positive DT Positive Progesterone Positive Table. L/MS/MS dmrm parameters and retention times for internal standards. Analyte RT (min) Precursor ion (m/z) Frag (V) Product ion Quant ion (m/z) E (V) Aldosterone d Positive ortisol d Positive ortisone d Positive orticosterone d Positive -Deoxycortisol d Positive Ionization mode β-estradiol d Negative Testosterone d Positive -Deoxycorticosterone d Positive Androstenedione Positive Estrone d Negative 1α Progesterone d Positive DT d Positive Progesterone d Positive

6 ounts A Peak ID 1. Aldosterone (1 ng/ml). ortisol (1 ng/ml). ortisone (1 ng/ml). orticosterone (1 ng/ml). -Deoxycortisol (1 ng/ml). β-estradiol (1 ng/ml). Testosterone (1 ng/ml). -Deoxycorticosterone (1 ng/ml). Androstenedione (1 ng/ml) 1. Estrone (1 ng/ml). 1α Progesterone (1 ng/ml) 1. DT (1 ng/ml) 1. Progesterone (1 ng/ml) ounts B Figure. Final dmrm chromatogram of 1 analyzed hormones in serum (A), with a zoomed in chromatogram (B) showing β-estradiol and estrone. oncentrations are provided in the legend. 1 1

7 ounts 1 1. A Peak ID 1. Aldosterone d (1 ng/ml). ortisol d (1 ng/ml). ortisone d (1 ng/ml). orticosterone d (1 ng/ml). -Deoxycortisol d (1 ng/ml). β-estradiol d (1 ng/ml). Testosterone d (1 ng/ml). -Deoxycorticosterone d (1 ng/ml). Androstenedione 1 (1 ng/ml) 1. Estrone d (1 ng/ml). 1α Progesterone d (1 ng/ml) 1. DT d (1 ng/ml) 1. Progesterone d (1 ng/ml) ounts 1 B Figure. Final dmrm chromatogram of 1 analyzed hormone internal standards in serum (A) with a zoomed in chromatogram (B) showing β-estradiol d and estrone d. oncentrations are provided in the legend.

8 Results and Discussion SPE ptimization When developing an SPE method, it is important to use a strong enough wash solvent to remove matrix interferences, while ensuring your analytes of interest remain retained on the cartridge. By collecting and analyzing the wash eluent from each of the candidate wash solvents (1% water, 1% Me in water, % Me in water, and so forth), hormone loss can be determined. After evaluation, a % Me in water wash was chosen as the optimal wash for this protocol. Similar to the wash step, the elution solvent can be optimized as well. The goal of the elution step is to choose a solvent that is strong enough to elute all the target analytes, without eluting matrix interferences that may be retained on the sorbent during the SPE procedure. For this application note, % Me in water, % Me in water, 1% Me, and / Me/AN elution solvents were evaluated. The 1% Me elution solvent provided the highest hormone recoveries, and was therefore chosen for this application. L/MS/MS ptimization During initial L/MS/MS method development, hormones were analyzed: aldosterone, cortisol, cortisone, corticosterone, -deoxycortisol, androstenedione, -deoxycorticosterone, testosterone, 1α progesterone, DT (negative mode), and progesterone. Two negative mode analytes (β-estradiol and estrone) were later added to the method, and therefore were not included in method development chromatograms. An Agilent InfinityLab Poroshell 1 E- column (.1 mm,. µm) was compared to an Agilent InfinityLab Poroshell 1 E-1 column (.1 mm,. µm) (Figure ). The shorter alkyl chain of the E- delivered slightly shorter retention times for the hormones, while still separating all isobaric pairs. onditions Mobile phase A: Water +.1% formic acid Mobile phase B: Methanol +.1% formic acid Gradient program: minutes: % mobile phase B to % mobile phase B,.1 minutes: % mobile phase B, hold ounts ounts A Agilent InfinityLab Poroshell 1 E-,.1 mm x mm,. µm 1 B Agilent InfinityLab Poroshell 1 E-1,.1 mm x mm,. µm Figure. omparison of separation and retention for hormones (1 ng/ml) using Agilent InfinityLab Poroshell 1 E and E-1 columns. β-estradiol and estrone were added to the final method after this chromatogram was generated, and are not included in this chromatogram., Total run time: 1 minutes + 1 minute post-time Flow rate:. ml/min Injection volume: 1 µl Peak ID 1. Aldosterone. ortisol. ortisone. orticosterone. -Deoxycortisol. Androstenedione. -Deoxycorticosterone. Testosterone. 1α Progesterone 1. DT 1. Progesterone 1

9 Formic acid (.1%), ammonium hydroxide (.%, p 1.), and ammonium fluoride (p.) mobile phases were also evaluated (Figures -). igh p mobile phase with ammonium hydroxide (.%) has been shown to improve ionization of hormones analyzed in negative mode [1]. Ammonium fluoride has been shown to improve responses in both positive and negative modes [,]. Since most silica-based L columns are not recommended for mobile phases with a p higher than or, an Agilent InfinityLab Poroshell P- column was used for evaluation. This column is stable up to a p of, and provides very similar selectivity to an InfinityLab Poroshell 1 E- column. Therefore, the InfinityLab Poroshell P- column was used for method optimization with both ammonium hydroxide and ammonium fluoride mobile phases, and chosen for the final analytical method. Ammonium fluoride (1 mm) mobile phase yielded the highest analyte responses, and was used for the final chromatographic method. onditions Mobile phase A: Mobile phase B: ounts 1 Peak ID.% Ammonium hydroxide in water 1. Aldosterone.1% Formic acid in water DT % Formic acid in water or.% ammonium hydroxide in water Methanol Gradient program: minutes: % mobile phase B to % mobile phase B minutes: % mobile phase B to % mobile phase B Total run time: 1 minutes + 1 minute post-time Flow rate:. ml/min Injection volume: 1 µl ortisol ortisone orticosterone -Deoxycortisol Androstenedione -Deoxycorticosterone Testosterone 1α Progesterone. Progesterone Figure. hromatogram for a 1 ng/ml hormone sample with.1% formic acid in water (green trace) and.% ammonium hydroxide in water (red trace) using an Agilent InfinityLab Poroshell P-,.1 mm mm,. µm column. The L/MS/MS gradient was kept the same for comparison purposes. β-estradiol and estrone were added to the method after these chromatograms were generated, and are not included.

10 onditions Mobile phase A: Mobile phase B: ounts mm ammonium fluoride in water or. % ammonium hydroxide in water Methanol Gradient program: minutes: % mobile phase B to % mobile phase B minutes: % mobile phase B to % mobile phase B Total run time: 1 minutes + 1 minute post-time Flow rate:. ml/min Injection volume: 1 µl 1 mm Ammonium fluoride in water.% Ammonium hydroxide in water Peak ID 1. Aldosterone. ortisol. ortisone. orticosterone. -Deoxycortisol. Androstenedione. -Deoxycorticosterone. Testosterone. 1α Progesterone 1. DT. Progesterone Figure. Total ion chromatogram for a 1 ng/ml hormone sample with 1 mm ammonium fluoride in water (black trace), and.% ammonium hydroxide in water (red trace). An Agilent InfinityLab Poroshell P-,.1 mm mm,. µm was used. The L/MS/MS gradient was kept the same for comparison purposes. β-estradiol and estrone were added to the method after these chromatograms were generated, and are not included. 1

11 ounts DT, 1 ng/ml 1 mm Ammonium fluoride in water.% Ammonium hydroxide in water.1% Formic acid in water Figure. MRM chromatogram overlay for DT (1 ng/ml; negative mode) with three mobile phases: 1 mm ammonium fluoride in water (black),.% ammonium hydroxide in water (red), and.1% formic acid in water (green). An Agilent InfinityLab Poroshell P-,.1 mm mm,. µm was used. Gradient conditions are provided in the descriptions of Figures and. Recovery and reproducibility Recovery and reproducibility were evaluated by extracting prespiked and blank serum samples using the SPE protocol previously described. Serum samples were prespiked with a concentration of 1 ng/ml for β-estradiol, estrone, and DT. The remaining 1 hormones were prespiked at a concentration of 1 ng/ml. Blank and spiked serum samples were extracted using the SPE protocol. Blank samples were postspiked after final dry down at a concentration of 1 and 1 ng/ml, as described earlier. Percent recovery and RSD values were determined by comparing average peak areas for prespiked and postspiked samples (n = ). Table. Percent recovery and RSD values (shown in parentheses) are provided (n = ). Analyte Fortified concentration (ng/ml) % Recovery (RSD) Aldosterone 1 (.) ortisol 1 1 (.) ortisone 1 (.) orticosterone 1 (.1) -Deoxycortisol 1 (.) β-estradiol 1 1 (.) Testosterone 1 (.) -Deoxycorticosterone 1 1 (.) Androstenedione 1 (.) Estrone 1 (.) 1α Progesterone 1 1 (.) DT 1 (.) Progesterone 1 (.)

12 onclusions A method was developed for the determination of 1 hormones, and their respective internal standards in serum using Agilent Bond Elut Plexa SPE cartridges. Analyte response with an Agilent triple quadrupole L/MS/MS operating in both positive and negative mode was greatly enhanced with the addition of 1 mm ammonium fluoride. An Agilent InfinityLab Poroshell P- (.1 mm mm,. µm) column yielded fast run times and baseline separation of three isobaric pairs, allowing for more reproducible integration and quantification. The wide p range makes it an ideal column choice for method development. The method provided excellent recoveries (-1%) and low RSD values (.-.%). Agilent Bond Elut Plexa SPE products require little method development and are simple to use, making them the ideal choice for biological analysis. References 1. Fu, R.; Zhai, A. Determination of ormones in Drinking Water by L/MS/MS Using an Agilent InfinityLab Poroshell P olumn (EPA ); Application note, Agilent Technologies, Inc. Publication number 1 EN, 1.. indle, R. Improved Analysis of Trace ormones in Drinking Water by L/MS/MS (EPA ) using the Agilent Triple Quadrupole L/MS; Application note, Agilent Technologies, Inc. Publication number 1 EN, 1.. Fiers, T.; asetta, B.; Bernaert, B.; Vandersypt, E.; Debock, M.; Kaufman, J. Development of a highly sensitive method for the quantification of estrone and estradiol in serum by liquid chromatography tandem mass spectrometry without derivatization. Journal of hromatography B. 1,,. For More Information These data represent typical results. For more information on our products and services, visit our Web site at For Research Use nly. Not for use in diagnostic procedures. Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material. Information, descriptions, and specifications in this publication are subject to change without notice. Agilent Technologies, Inc., 1 Printed in the USA May, 1 1-EN

Determination of Hormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell HPH Column (EPA 539)

Determination of Hormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell HPH Column (EPA 539) Determination of ormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell P Column (EPA 539) Application Note Environmental Authors Rong-jie Fu and Chen-ao (Andy) Zhai Agilent Technologies

More information

Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS

Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS Application Note Food Testing and Agriculture Author Chen-Hao (Andy) Zhai and Rong-jie Fu Agilent Technologies (Shanghai)

More information

Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS

Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Application Note Clinical Research Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Authors Yanan Yang 1, Victor Mandragon 2, and Peter Stone 1 1

More information

Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS

Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 129 Infi nity LC, and 646 Triple Quadrupole LC/MS Application Note Clinical Research Authors Linda Côté and Christophe

More information

LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode

LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode Application Note Authors Siji Joseph Agilent Technologies India Pvt. Ltd.

More information

Aminoglycosides in Milk Using Agilent Bond Elut Plexa SPE, Agilent Poroshell 120, and LC/Tandem MS

Aminoglycosides in Milk Using Agilent Bond Elut Plexa SPE, Agilent Poroshell 120, and LC/Tandem MS Aminoglycosides in Milk Using Agilent Bond Elut Plexa SPE, Agilent Poroshell 120, and LC/Tandem MS Application ote Food Testing & Agriculture Author Andy Zhai Agilent Technologies, Inc. Shanghai Co. Ltd.

More information

Analysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography

Analysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography Application Note Inorganic Ions, Water Testing, Minerals, Metals, Basic Chemicals Analysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography Authors Anne Mack, Adam Bivens

More information

Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry

Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Application Note Food Authors Jianqiu Mi, Zhengxiang Zhang, Zhixu Zhang,

More information

SAMHSA-Compliant LC/MS/MS Analysis of Phencyclidine in Urine with Agilent Bond Elut Plexa PCX and Agilent Poroshell 120

SAMHSA-Compliant LC/MS/MS Analysis of Phencyclidine in Urine with Agilent Bond Elut Plexa PCX and Agilent Poroshell 120 SAMHSA-Compliant LC/MS/MS Analysis of Phencyclidine in Urine with Agilent Bond Elut Plexa PCX and Agilent Poroshell 120 Application Note Forensic Toxicology Authors Irina ioumaeva, John M. Hughes Agilent

More information

Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System

Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Application Note Authors Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon, and

More information

LC/MS/MS of Fungicides and Metabolites in Orange Juice with Agilent Bond Elut Plexa and Poroshell 120

LC/MS/MS of Fungicides and Metabolites in Orange Juice with Agilent Bond Elut Plexa and Poroshell 120 LC/MS/MS of Fungicides and Metabolites in Orange Juice with Agilent Bond Elut Plexa and Poroshell 10 Application Note Food Testing & Agriculture Author Irina Dioumaeva Agilent Technologies, Inc. Abstract

More information

Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System

Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System Application Note Authors Mohamed Youssef and Vaughn P. Miller Agilent

More information

LC/MS/MS of Fungicides and Metabolites in Apple Juice with Agilent Bond Elut Plexa and Poroshell 120

LC/MS/MS of Fungicides and Metabolites in Apple Juice with Agilent Bond Elut Plexa and Poroshell 120 LC/MS/MS of Fungicides and Metabolites in Apple Juice with Agilent Bond Elut Plexa and Poroshell 120 Application Note Food Testing & Agriculture Author Irina Dioumaeva Agilent Technologies, Inc. Abstract

More information

High Sensitivity HPLC Analysis of Perchlorate in Tap Water Using an Agilent 6460 Triple Quadrupole LC/MS System

High Sensitivity HPLC Analysis of Perchlorate in Tap Water Using an Agilent 6460 Triple Quadrupole LC/MS System High Sensitivity HPLC Analysis of Perchlorate in Tap Water Using an Agilent 66 Triple Quadrupole LC/MS System Application Note Environmental Authors Don Noot, Matthew Noestheden, and Ralph Hindle Vogon

More information

Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX

Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX Technical Note Agilent s SampliQ WAX provides Applications for strongly acidic, acidic and neutral compounds Excellent

More information

Determination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry

Determination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry Determination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry Application Note Doping Control Authors Prof. Maria Kristina Parr Freie Universität Berlin Institute

More information

Extraction of Methylmalonic Acid from Serum Using ISOLUTE. SAX Prior to LC-MS/MS Analysis

Extraction of Methylmalonic Acid from Serum Using ISOLUTE. SAX Prior to LC-MS/MS Analysis Application Note AN89.V.1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Page 1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Prior to LC-MS/MS Analysis Sample Preparation

More information

Analysis of Stachydrine in Leonurus japonicus Using an Agilent ZORBAX RRHD HILIC Plus Column with LC/ELSD and LC/MS/MS

Analysis of Stachydrine in Leonurus japonicus Using an Agilent ZORBAX RRHD HILIC Plus Column with LC/ELSD and LC/MS/MS Analysis of Stachydrine in Leonurus japonicus Using an Agilent ZORBAX RRHD HILIC Plus Column with LC/ELSD and LC/MS/MS Application Note Traditional Chinese Medicine Author Rongjie Fu Agilent Technologies

More information

Sensitive Femtogram Determination of Aflatoxins B 1 , B 2 , G 1. and G 2. in Food Matrices using Triple Quadrupole LC/MS. Authors.

Sensitive Femtogram Determination of Aflatoxins B 1 , B 2 , G 1. and G 2. in Food Matrices using Triple Quadrupole LC/MS. Authors. Sensitive Femtogram Determination of Aflatoxins B 1, B 2, G 1 and G 2 in Food Matrices using Triple Quadrupole LC/MS Application Note Food Safety Authors Yang Chen and Jack Cappozzo National Center for

More information

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS Christopher Borton AB SCIEX Golden, Colorado verview Described here is the analysis of

More information

Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS

Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 643 LC/MS/MS Application Note Forensic Toxicology Authors Jason Hudson, Ph.D., James Hutchings, Ph.D., and Rebecca Wagner,

More information

Using UHPLC-Triple Quadrupole MS/MS to Detect the Presence of Bark Extract and Yohimbine Adulteration in Dietary Supplements and Botanicals

Using UHPLC-Triple Quadrupole MS/MS to Detect the Presence of Bark Extract and Yohimbine Adulteration in Dietary Supplements and Botanicals Using UHPLC-Triple Quadrupole MS/MS to Detect the Presence of Bark Extract and Adulteration in Dietary Supplements and Botanicals Application Note Food Testing & Agriculture Authors James Neal-Kababick

More information

Analysis of Low-Calorie Sweeteners by Liquid Chromatography-Tandem Mass Spectrometry

Analysis of Low-Calorie Sweeteners by Liquid Chromatography-Tandem Mass Spectrometry Analysis of Low-Calorie Sweeteners by Liquid Chromatography-Tandem Mass Spectrometry Application Note Food safety Authors Ismael Flores and Carlos Sepulveda Agrolab México Km 7 Carretera Pachuca-Actopan

More information

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS Application Note EPA Method 535 EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS API 3200 LC/MS/MS System Overview Described here is the

More information

Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS. Application. Authors. Abstract.

Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS. Application. Authors. Abstract. Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS Application Food Analysis Authors Sheher Mohsin Agilent Technologies, Inc. Schaumburg, Il USA Michael Zumwalt

More information

Determination of Chlorinated Acid Herbicides in Soil by LC/MS/MS Application Note

Determination of Chlorinated Acid Herbicides in Soil by LC/MS/MS Application Note Determination of Chlorinated Acid Herbicides in Soil by LC/MS/MS Application Note Environmental Author Chin-Kai Meng Agilent Technologies 85 Centerville Road Wilmington, DE 988-6 USA Abstract Chlorinated

More information

Exceptional Selectivity of Agilent ZORBAX Eclipse Plus Phenyl-Hexyl Columns to Separate Estrogens

Exceptional Selectivity of Agilent ZORBAX Eclipse Plus Phenyl-Hexyl Columns to Separate Estrogens Exceptional Selectivity of Agilent ZORBAX Eclipse Plus Phenyl-exyl Columns to Separate Estrogens Application Note Pharmaceutical, Environmental Authors John W. enderson Jr. and William J. Long Agilent

More information

Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma

Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Jon Bardsley 1, James Goldberg 2 1 Thermo Fisher Scientific, Runcorn, UK; 2 Thermo Fisher Scientific, West Palm Beach,

More information

Detection of Trace Level Pharmaceuticals in Drinking Water by Online SPE Enrichment with the Agilent 1200 Infinity Series Online-SPE Solution

Detection of Trace Level Pharmaceuticals in Drinking Water by Online SPE Enrichment with the Agilent 1200 Infinity Series Online-SPE Solution Detection of Trace Level Pharmaceuticals in Drinking Water by nline SPE Enrichment with the Agilent Infinity Series nline-spe Solution Application Note Environmental Author Edgar Naegele Agilent Technologies,

More information

Application Note. Abstract. Authors. Environmental

Application Note. Abstract. Authors. Environmental Determination of Acidic Herbicides using an Agilent 6460 Triple Quadrupole LC/MS Equipped with Agilent Jet Stream Technology and Direct Aqueous Injection, for Potable and Environmental Samples Application

More information

Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS

Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS Application Note Drug Discovery Authors Srividya Kailasam Agilent

More information

Determination of 24 PAHs in Drinking Water

Determination of 24 PAHs in Drinking Water Determination of 24 PAHs in Drinking Water Application Note Food Testing and Agriculture Authors M.C. Díaz Ramos, A. Suárez, A. Rúbies, and R. Companyó Laboratori de l Agencia de Salut Publica de Barcelona

More information

LC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges

LC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges LC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges Application ote Food Testing & Agriculture Author Andy Zhai Agilent Technologies Shanghai Co. Ltd. Abstract This application

More information

The Agilent InfinityLab 2D-LC Solution with Active Solvent Modulation

The Agilent InfinityLab 2D-LC Solution with Active Solvent Modulation Technical Overview The Agilent InfinityLab D-LC Solution with Active Solvent Modulation Achieving Improved Resolution and Sensitivity for Challenging Combinations of Separation Conditions Author Sonja

More information

Application Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors

Application Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors Trace-Level Analysis of Melamine in Milk Products on Agilent 789A/5975C GC/MSD Using a ew Agilent J&W DB-5ms Ultra Inert Column and SampliQ SCX Cartridges Application ote Gas Chromatography/Mass Spectrometry/Food

More information

LC-MS/MS Method for the Determination of Diclofenac in Human Plasma

LC-MS/MS Method for the Determination of Diclofenac in Human Plasma LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core

More information

Analysis of Positional Isomers of Lapatinib with Agilent Poroshell 120 PFP Columns

Analysis of Positional Isomers of Lapatinib with Agilent Poroshell 120 PFP Columns Analysis of Positional Isomers of Lapatinib with Agilent Poroshell 2 PFP Columns Application ote Small Molecule Pharmaceuticals Authors Rongjie Fu Agilent Technologies (Shanghai) Co., Ltd Linhai Zhang

More information

Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research

Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Heather A Brown, 1 Claudia Rossi, 2 and Lisa J Calton 1 1 Waters Corporation, Wilmslow, UK 2

More information

Separation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers

Separation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers Application Note Forensic and Toxicology - Criminalistics Separation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers Using the Agilent 126 Infinity II SFC and Detection by Coupled

More information

Analysis of Trace (mg/kg) Thiophene in Benzene Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application

Analysis of Trace (mg/kg) Thiophene in Benzene Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application Analysis of Trace (mg/kg) Thiophene in Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application Petrochemical Authors James D. McCurry and Bruce D. Quimby Agilent Technologies

More information

High-Throughput Protein Quantitation Using Multiple Reaction Monitoring

High-Throughput Protein Quantitation Using Multiple Reaction Monitoring High-Throughput Protein Quantitation Using Multiple Reaction Monitoring Application Note Authors Ning Tang, Christine Miller, Joe Roark, Norton Kitagawa and Keith Waddell Agilent Technologies, Inc. Santa

More information

Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes

Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes Application Note Food Safety Author Sunil Kulkarni Agilent Technologies

More information

Fast Screening Methods for Steroids by HPLC with Agilent Poroshell 120 Columns

Fast Screening Methods for Steroids by HPLC with Agilent Poroshell 120 Columns Fast Screening Methods for Steroids by HPLC with Agilent Poroshell 2 Columns Application Note Pharma, BioPharma, and Clinical Research Author William Long Agilent Technologies, Inc. Introduction Steroids

More information

Analysis of Cannabinoids and Amphetamines in Serum by RRLC/Triple Quadrupole Mass Spectrometry Using a Multimode Ion Source. Application.

Analysis of Cannabinoids and Amphetamines in Serum by RRLC/Triple Quadrupole Mass Spectrometry Using a Multimode Ion Source. Application. Analysis of Cannabinoids and Amphetamines in Serum by RRLC/Triple Quadrupole Mass Spectrometry Using a Multimode Ion Source Application Toxicology Authors Jürgen Wendt Agilent Technologies Sales and Support

More information

Agilent 1200 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity

Agilent 1200 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity Agilent 12 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity A case study with degraded omeprazole drug product Application Note Small Molecule

More information

Determination of Pharmaceuticals in Water by SPE and LC/MS/MS in Both Positive and Negative Ion Modes Application

Determination of Pharmaceuticals in Water by SPE and LC/MS/MS in Both Positive and Negative Ion Modes Application Determination of Pharmaceuticals in Water by SPE and LC/MS/MS in Both Positive and Negative Ion Modes Application Environmental Authors Chin-Kai Meng Agilent Technologies, Inc. 85 Centerville Road Wilmington,

More information

High-Resolution Sampling 2D-LC for Pharmaceutical Impurity Analysis

High-Resolution Sampling 2D-LC for Pharmaceutical Impurity Analysis High-Resolution Sampling D-LC for Pharmaceutical Impurity Analysis Detection of Impurities Hidden Under the API Peak at Relevant Levels Application Note Small Molecule Pharmaceuticals and Generics Authors

More information

Analysis of Positional Isomers with Agilent Poroshell 120 PFP Columns

Analysis of Positional Isomers with Agilent Poroshell 120 PFP Columns Analysis of Positional Isomers with Agilent Poroshell PFP olumns Application ote Environmental Authors William Long and Jonathan Horton Agilent Technologies, Inc. Introduction The Agilent Poroshell PFP

More information

Accurate Mass Analysis of Hydraulic Fracturing Waters: Identification of Polyethylene Glycol Surfactants by LC/Q-TOF-MS

Accurate Mass Analysis of Hydraulic Fracturing Waters: Identification of Polyethylene Glycol Surfactants by LC/Q-TOF-MS Accurate Mass Analysis of Hydraulic Fracturing Waters: Identification of Polyethylene Glycol Surfactants by LC/Q-TOF-MS Application Note Authors E. Michael Thurman and Imma Ferrer Center for Environmental

More information

Anaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection

Anaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection Anaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection Application Note Food Safety Author Andy Zhai Agilent technologies Co., Ltd. 412 Yinglun Road Shanghai,

More information

Determination of Alkaloids in Goldenseal Using Agilent Bond Elut Plexa Solid Phase Extraction Sorbent for Cleanup and HPLC-DAD Analysis

Determination of Alkaloids in Goldenseal Using Agilent Bond Elut Plexa Solid Phase Extraction Sorbent for Cleanup and HPLC-DAD Analysis Determination of Alkaloids in Goldenseal Using Agilent Bond Elut Plexa Solid Phase Extraction Sorbent for Cleanup and HPLC-DAD Analysis Application Note Dietary Supplement Author Kediemetse Mothibedi,

More information

The Agilent 1260 Infinity Analytical SFC System with Time-of-Flight Mass Spectrometric Detection

The Agilent 1260 Infinity Analytical SFC System with Time-of-Flight Mass Spectrometric Detection Application Note Small Molecules The Agilent 26 Infinity Analytical SFC System with Time-of-Flight Mass Spectrometric Detection Method Development Using Method Scouting Wizard Authors Stefan Bieber Analytical

More information

A New Polymer-based SPE Sorbent to Reduce Matrix Effects in Bioanalytical LC-MS/MS

A New Polymer-based SPE Sorbent to Reduce Matrix Effects in Bioanalytical LC-MS/MS A ew Polymer-based SPE Sorbent to Reduce Matrix Effects in Bioanalytical LC-MS/MS M. Cleeve, L. Williams, A. owells, G. Davies, S. Merriman, S. Plant,. Gooding, J. Labadie, R. Johnston, M. Burke 1, R.

More information

APPLICATIONS TN Increased Capabilities for the Analysis of Hormones in Drinking and Waste Water Using Solid Phase Extraction (SPE) and LC/MS/MS

APPLICATIONS TN Increased Capabilities for the Analysis of Hormones in Drinking and Waste Water Using Solid Phase Extraction (SPE) and LC/MS/MS Increased Capabilities for the Analysis of Hormones in Drinking and Waste Water Using Solid Phase Extraction (SPE) and LC/MS/MS Xianrong (Jenny) Wei Senior Scientist Jenny is a Senior Scientist in Phenomenex

More information

Application Note. Author. Abstract. Pharmaceutical QA/QC. Siji Joseph Agilent Technologies, Inc. Bangalore, India

Application Note. Author. Abstract. Pharmaceutical QA/QC. Siji Joseph Agilent Technologies, Inc. Bangalore, India Effective use of pharmacopeia guidelines to reduce cost of chromatographic analysis Optimized, cost-effective HPLC analysis of atorvastatin by varying column dimensions within the USP allowed limts

More information

Accurate quantification of oleanolic acid and ursolic acid in traditional chinese medicine

Accurate quantification of oleanolic acid and ursolic acid in traditional chinese medicine pplication Note Small Molecule Pharmaceuticals, Generics ccurate quantification of oleanolic acid and ursolic acid in traditional chinese medicine igh-resolution sampling D-LC uthors Rongjie Fu and Xinlei

More information

Accurate Mass Measurement for Intact Proteins using ESI-oa-TOF. Application Note. Donghui Yi and Christine Miller Agilent Technologies

Accurate Mass Measurement for Intact Proteins using ESI-oa-TOF. Application Note. Donghui Yi and Christine Miller Agilent Technologies Accurate Mass Measurement for Intact Proteins using ESI-oa-TOF Application Note Donghui Yi and Christine Miller Jon D. Williams, GlaxoSmithKline Introduction Mass spectrometry (MS) has become a core technology

More information

Automated Switching Between 1D-LC and Comprehensive 2D-LC Analysis

Automated Switching Between 1D-LC and Comprehensive 2D-LC Analysis Automated Switching Between D-LC and Comprehensive D-LC Analysis The Agilent 90 Infinity D-LC Solution Technical Overview Author Sonja Krieger Agilent Technologies, Inc. Waldbronn, Germany Abstract This

More information

Analysis of Phthalates in Body Wash using Solid-Supported Liquid-Liquid Extraction

Analysis of Phthalates in Body Wash using Solid-Supported Liquid-Liquid Extraction Analysis of Phthalates in Body Wash using Solid-Supported Liquid-Liquid Extraction Application Note Consumer Products Authors Devon C. Zimmerman, Henry F. Rossi III, Jacqueline Rizzo, Daniel W. Keating,

More information

Fractionation of Acidic, Basic, and Neutral Drugs from Plasma with an SPE Mixed Mode Strong Cation Exchange Polymeric Resin (Agilent SampliQ SCX)

Fractionation of Acidic, Basic, and Neutral Drugs from Plasma with an SPE Mixed Mode Strong Cation Exchange Polymeric Resin (Agilent SampliQ SCX) Fractionation of Acidic, Basic, and Neutral Drugs from Plasma with an SPE Mixed Mode Strong Cation Exchange Polymeric Resin (Agilent SampliQ SCX) Application Note Forensic Toxicology Authors Bellah. Pule,

More information

Solid Phase Extraction Method Development Tips and Tricks

Solid Phase Extraction Method Development Tips and Tricks Solid Phase Extraction Method Development Tips and Tricks Carol Haney Ball, Ph.D. Application Scientist Agilent Technologies, Inc. February 3, 2009 Sources of Error Generated and Time Spent During a Typical

More information

Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method

Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method By Sharon Lupo and Frances Carroll Abstract The analysis of synthetic cannabinoids and their metabolites

More information

Quantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 1260 Infinity Binary LC system with UV detection

Quantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 1260 Infinity Binary LC system with UV detection Quantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 126 Infinity Binary LC system with UV detection Application Note Food Author Srividya Kailasam Agilent Technologies,

More information

Determination of Off-Odor Compounds in Drinking Water Using an SPME Device with Gas Chromatography and Mass Spectrometry

Determination of Off-Odor Compounds in Drinking Water Using an SPME Device with Gas Chromatography and Mass Spectrometry Application Note Environmental Determination of Off-Odor Compounds in Drinking Water Using an SPME Device with Gas Chromatography and Mass Spectrometry Authors Ye Kong and Zhe Cao Agilent Technologies,

More information

Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS

Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1

More information

Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative. Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish.

Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative. Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish. 1 Supporting Information 2 3 Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish. 4 5 John Moreman, Okhyun

More information

Automated Sample Preparation in Quality Control of Eye-Drop Formulation

Automated Sample Preparation in Quality Control of Eye-Drop Formulation Automated Sample Preparation in Quality Control of Eye-Drop Formulation Application Note Authors Bart Tienpont, Maria Rambla Alegre, Frank David, and Pat Sandra Research Institute for Chromatography Kennedypark

More information

Application Note. Author. Abstract. Food Testing & Agriculture. Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany

Application Note. Author. Abstract. Food Testing & Agriculture. Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany Analysis of Pesticides by Supercritical Fluid Chromatography/ Mass Spectrometry Optimizing the Agilent 126 Infi nity Analytical SFC System in Combination with the Agilent 646 Triple Quadrupole LC/MS Application

More information

Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer

Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer 38 Current Trends in Mass Spectrometry November 6 Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer This article demonstrates the improved

More information

Clinical Toxicology. Biomass Component Extraction: The uneaten cooked plant specimen was prepared for

Clinical Toxicology. Biomass Component Extraction: The uneaten cooked plant specimen was prepared for Clinical Toxicology Page of 0 Materials and Methods Biomass Component Extraction: The uneaten cooked plant specimen was prepared for chemical analysis as follows. The sample was frozen, diced, pulverized

More information

Application Note. Pharmaceutical QA/QC. Author. Abstract. Siji Joseph Agilent Technologies, Inc. Bangalore, India

Application Note. Pharmaceutical QA/QC. Author. Abstract. Siji Joseph Agilent Technologies, Inc. Bangalore, India Reducing analysis time and solvent consumption for isocratic USP assay methods with current and proposed USP guidelines using the Agilent 129 Infinity LC System An efficient way to reduce cost of analysis

More information

Simultaneous Determination of 26 Mycotoxins in Sesame Butter Using Modified QuEChERS Coupled with UHPLC-ESI/Triple Quadrupole Mass Spectrometry

Simultaneous Determination of 26 Mycotoxins in Sesame Butter Using Modified QuEChERS Coupled with UHPLC-ESI/Triple Quadrupole Mass Spectrometry Simultaneous Determination of 2 Mycotoxins in Sesame Butter Using Modified QuEChERS Coupled with UHPLC-ESI/Triple Quadrupole Mass Spectrometry Application Note Food Authors Ying Liu, Shen Han, Pei-Yue

More information

Pharmaceutical Impurity Identification and Profiling Using Agilent Q-TOF LC/MS Combined with Advanced MassHunter Data Processing Software

Pharmaceutical Impurity Identification and Profiling Using Agilent Q-TOF LC/MS Combined with Advanced MassHunter Data Processing Software Pharmaceutical Impurity Identification and Profiling Using Agilent Q-TF LC/MS Combined with Advanced Massunter Data Processing Software Application Note Authors Siji Joseph Agilent Technologies LSCI Bangalore,

More information

Measuring Drug-to-Antibody Ratio (DAR) for Antibody-Drug Conjugates (ADCs) with UHPLC/Q-TOF

Measuring Drug-to-Antibody Ratio (DAR) for Antibody-Drug Conjugates (ADCs) with UHPLC/Q-TOF Measuring Drug-to-Antibody Ratio (DAR) for Antibody-Drug Conjugates (ADCs) with UHPLC/Q-TOF Application Note Biopharma Author Shuai Zuo Tao Bo Suresh Babu C.V. Agilent Technologies, Inc. Abstract In this

More information

Separation of Explosives in EPA 8330: Column Choices Optimize Speed, Resolution, and Solvent Use. Application. Authors. Abstract.

Separation of Explosives in EPA 8330: Column Choices Optimize Speed, Resolution, and Solvent Use. Application. Authors. Abstract. Separation of Explosives in EPA 833: Column Choices Optimize Speed, Resolution, and Solvent Use Application Environmental Authors John W. Henderson Jr. and William J. Long Agilent Technologies, Inc. 28

More information

Speciation of Bromine Compounds in Ozonated Drinking Water using Ion Chromatography and Inductively Coupled Plasma Mass Spectrometry

Speciation of Bromine Compounds in Ozonated Drinking Water using Ion Chromatography and Inductively Coupled Plasma Mass Spectrometry APPLICATION NOTE Speciation of Bromine Compounds in Ozonated Drinking Water using Ion Chromatography and Inductively Coupled Plasma Mass Spectrometry AN43227 Antonella Guzzonato 1, Shona McSheehy Ducos

More information

Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS

Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1

More information

Extraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE. Myco Prior to LC-MS/MS Analysis

Extraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE. Myco Prior to LC-MS/MS Analysis Application Note AN785 Extraction of Aflatoxins and chratoxin From Dried Chili Using ISLUTE Myco prior to LC-MS/MS Analysis Page Extraction of Aflatoxins and chratoxin from Dried Chili Using ISLUTE Myco

More information

Mixed-Mode, Weak Anion-Exchange, Solid-Phase Extraction Method for the Extraction of Niflumic Acid from Human Plasma

Mixed-Mode, Weak Anion-Exchange, Solid-Phase Extraction Method for the Extraction of Niflumic Acid from Human Plasma Mixed-Mode, Weak Anion-Exchange, Solid-Phase Extraction Method for the Extraction of Niflumic Acid from Human Plasma Ken Meadows, Thermo Fisher Scientific, Runcorn, UK Application Note 20789 Key Words

More information

Agilent 1290 Infinity Quaternary LC Stepwise Transfer to Methods with MS-Compatible Mobile Phases

Agilent 1290 Infinity Quaternary LC Stepwise Transfer to Methods with MS-Compatible Mobile Phases Agilent 129 Infinity Quaternary LC Stepwise Transfer to Methods with MS-Compatible Mobile Phases Technical Overview Author A.G.Huesgen Agilent Technologies, Inc. Waldbronn, Germany Abstract The Agilent

More information

LC-MS/MS Analysis of Phytocannabinoids and their

LC-MS/MS Analysis of Phytocannabinoids and their POSTER NOTE 64922 LC-MS/MS Analysis of Phytocannabinoids and their LC-MS/MS Analysis of Phytocannabinoid Metabolites in Urine, Oral Fluid and Blood Rory M Doyle, Sherry Gregory*, Thermo Fisher Scientific,

More information

Development of analytical methods for Catecholamines, Metanephrines and related metabolites in urine and plasma matrices

Development of analytical methods for Catecholamines, Metanephrines and related metabolites in urine and plasma matrices Development of analytical methods for Catecholamines, Metanephrines and related metabolites in urine and plasma matrices From sample preparation to LC/MS/MS Linda Côté Christophe Deckers Agilent Technologies

More information

MassHunter METLIN Metabolite PCD/PCDL Quick Start Guide

MassHunter METLIN Metabolite PCD/PCDL Quick Start Guide MassHunter METLIN Metabolite PCD/PCDL Quick Start Guide What is the MassHunter METLIN Metabolite PCD/PCDL? 2 Where to find more information 2 Kit Content 3 Installation 5 Before you start 5 Install MassHunter

More information

Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF

Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF application Note Liquid Chromatography/ Mass Spectrometry Author Sharanya Reddy PerkinElmer, Inc. Shelton, CT USA Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF

More information

Performance evaluation of the Agilent 1290 Infinity 2D-LC Solution for comprehensive two-dimensional liquid chromatography

Performance evaluation of the Agilent 1290 Infinity 2D-LC Solution for comprehensive two-dimensional liquid chromatography Performance evaluation of the Agilent 1290 Infinity 2D-LC Solution for comprehensive two-dimensional liquid chromatography Technical Overview 2D-LC Conventional 1D-LC Abstract This Technical Overview presents

More information

Identification and Quantitation of Pesticides in Chamomile and Ginger Extracts Using an Agilent 6460 Triple Quadrupole LC/MS system with Triggered MRM

Identification and Quantitation of Pesticides in Chamomile and Ginger Extracts Using an Agilent 6460 Triple Quadrupole LC/MS system with Triggered MRM Identification and Quantitation of Pesticides in Chamomile and Ginger Extracts Using an Agilent 646 Triple Quadrupole LC/MS system with Triggered MRM Application Note Authors Thomas Glauner Agilent Technologies,

More information

Application. Introduction. Authors. Abstract. Hydrocarbon Processing

Application. Introduction. Authors. Abstract. Hydrocarbon Processing Fast Analysis of Phenolic Antioxidants and Erucamide Slip Additives in Polypropylene Homopolymer Formulations Using Rapid Resolution Liquid hromatography (RRL) with Rapid Resolution High Throughput (RRHT)

More information

Performance characteristics of the Agilent 1290 Infinity Quaternary Pump

Performance characteristics of the Agilent 1290 Infinity Quaternary Pump Performance characteristics of the Agilent 129 Infinity Quaternary Pump Technical Overview Author A.G.Huesgen Agilent Technologies, Inc. Waldbronn, Germany Abstract This Technical Overview presents Proof

More information

Application. Authors. Abstract. Introduction. Hydrocarbon Processing

Application. Authors. Abstract. Introduction. Hydrocarbon Processing Fast Analysis Method for Rubber Chemical Antidegradants Using Rapid Resolution Liquid Chromatography (RRLC) Systems with Rapid Resolution High Throughput (RRHT) Columns Application Hydrocarbon Processing

More information

High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes

High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes Joe DiBussolo, Marta Kozak Thermo Fisher Scientific, San Jose, CA Application

More information

Determination of Enantiomeric Excess of Metolachlor from Chiral Synthesis using the Agilent 1260 Infinity Analytical SFC System

Determination of Enantiomeric Excess of Metolachlor from Chiral Synthesis using the Agilent 1260 Infinity Analytical SFC System Determination of Enantiomeric Excess of Metolachlor from hiral Synthesis using the Agilent 126 Infinity Analytical SF System Application Note Specialty hemicals Author Edgar Naegele Agilent Technologies,

More information

Analysis of DNPH-derivatized Aldehydes and Ketones using the Agilent 1220 Infinity LC System with Diode Array Detector

Analysis of DNPH-derivatized Aldehydes and Ketones using the Agilent 1220 Infinity LC System with Diode Array Detector Analysis of DNPH-derivatized Aldehydes and Ketones using the Agilent Infinity LC System with Diode Array Detector Application Note Author Sonja Schneider Agilent Technologies, Inc. Waldbronn, Germany [mau]

More information

HPLC Preparative Scaleup of Calcium Channel Blocker Pharmaceuticals Application

HPLC Preparative Scaleup of Calcium Channel Blocker Pharmaceuticals Application HPLC Preparative Scaleup of Calcium Channel Blocker Pharmaceuticals Application Pharmaceuticals Author Cliff Woodward and Ronald Majors Agilent Technologies, Inc. 2850 Centerville Road Wilmington, DE 19808

More information

Determination of Total Volatile Organic Compounds in Indoor Air Using Agilent 7667A mini TD and 7820A GC

Determination of Total Volatile Organic Compounds in Indoor Air Using Agilent 7667A mini TD and 7820A GC Determination of Total Volatile Organic Compounds in Indoor Air Using Agilent 77A mini TD and 70A GC Application Note Environmental Authors Tingting Bu, Xiaohua Li Agilent Technologies (Shanghai) Co.,

More information

Pesticides Analysis Using the Agilent 5977A Series GC/MSD

Pesticides Analysis Using the Agilent 5977A Series GC/MSD Pesticides Analysis Using the Agilent 5977A Series GC/MSD Application Note Food Testing and Agriculture Authors Jia-jia Wu and Wen-wen Wang Agilent Technologies Co. Ltd (China) Beijing, People s Republic

More information

Ultrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 1290 Infinity LC system

Ultrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 1290 Infinity LC system Ultrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 129 Infinity LC system Application Note Food and Beverages Author Srividya Kailasam

More information

Determination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS

Determination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS Determination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS Application Note Food Authors Gerd Vanhoenacker, Frank David,

More information

Application Note. Edgar Naegele. Abstract

Application Note. Edgar Naegele. Abstract Fast identification of main drug metabolites by quadrupole time-of-flight LC/MS Measuring accurate MS and MS/MS data with the Agilent 651 Q-TOF LC/MS and identification of main meta-bolites by comparison

More information

HPLC Winter Webinars Part 2: Sample Preparation for HPLC

HPLC Winter Webinars Part 2: Sample Preparation for HPLC HPLC Winter Webinars Part 2: Sample Preparation for HPLC Jon Bardsley, Application Chemist Thermo Fisher Scientific, Runcorn/UK The world leader in serving science What am I Going to Talk About? What do

More information

Determination of iopromide in environmental waters by ion chromatography-icp-ms

Determination of iopromide in environmental waters by ion chromatography-icp-ms Determination of iopromide in environmental waters by ion chromatography-icp-ms Application note Environmental Authors Armando Durazo, Tarun Anumol, and Shane A. Snyder Department of Chemical & Environmental

More information