Finnigan TSQ Quantum Discovery MAX

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1 m a s s s p e c t r o m e t r y Finnigan TSQ Quantum Discovery MAX Round-the-clock productivity Analyze Detect Measure Control

2 Lit Finnigan Sheet TSQ White Quantum Head Discovery MAX Extraordinary Value Finnigan TSQ Quantum Discovery MAX The high-performance, compact benchtop Finnigan TSQ Quantum Discovery MAX incorporates innovative technology for increased sensitivity, precision, ruggedness, and reliability. It is principally designed for high-throughput conditions such as environmental, clinical, and drug discovery workflow. With the Ion Max source, the Finnigan TSQ Quantum Discovery MAX addresses the need of these laboratories for more rugged and dependable LC-MS/MS to enable round-the-clock productivity. The combination of our patented HyperQuad quadrupole mass analyzer system, high efficiency ion transfer optics, and outstanding ion source robustness provides precise accurate quantitation even at the lowest levels. round-the-clock productivity, with precision,

3 ruggedness, and reliability

4 Finnigan TSQ Quantum Discovery MAX H-SRM High Transmission at High Resolution Selective Reaction Monitoring (SRM) is a widely used technique for performing quantitative assays using a triple quadrupole mass spectrometer. Highly-Selective Reaction Monitoring (H-SRM) allows the user to have more stringent tolerance for precursor ion selection, which leads to higher analyte selectivity. This can lead to lower LOQs, better precision and accuracy at the limits of assay detection. Using H-SRM, the precursor ion is selected with a mass tolerance of ±.1 m/z or less, which dramatically increases analyte selectivity and reduces method development time when chemical noise is an issue. Consequently, this reduces the likelihood of false positives. Thermo s hyperbolic triple quadrupoles are the only triple quadrupoles currently on the market capable of such mass selectivity without a significant loss of sensitivity. H-SRM is a standard feature on all Finnigan TSQ Quantum instruments. Relative Abundance Relative Abundance RT: 1.21 MA: SN: 8 TRADITIONAL SRM RT: 1.53 AA: NL: 4.31E4 Compound A SRM NL: 1.94E5 Internal Standard SRM Time (min) LC/ESI-SRM chromatograms of compound A (25 ng/ml) and internal standard spiked into the plasma matrix, under unit mass-resolution conditions H- SRM 2.91 Nominal Mean Conc. % Rel. Number of (ng/ml) (ng/ml) Error % CV Replicates (4) Relative Abundance RT: 1.23 AA: SN: 31 NL: 1.21E4 Compound A SRM Statistical data for the calibration curve of acrylamide Relative Abundance RT: 1.5 AA: NL: 6.13E4 Internal Standard SRM Time (min) LC/ESI-H-SRM chromatograms of compound A (25 ng/ml) and internal standard spiked into the plasma matrix precision

5 Robust Ionization Sources ESI, APPI, APCI, and Nanospray Ionization The Ion Max source has a revolutionary design, imparting superior performance and sensitivity. It is readily interchangeable between the Finnigan LTQ, the Finnigan LTQ FT, and the Finnigan LCQ ion traps. An aviation-grade titanium metal skimmer, newly contoured ion transfer tube for high ion transmission, and x, y, and z probe positioning elements make the Ion Max the most robust commercially available ion source. Patented Ion Max source ,, Area Ratio ,, %RSD = 2.9% Area Ratio ng/ml 2 1 1, ESI Robustness study on the Finnigan TSQ Quantum Ultra Ion Max. 3 injections of 2 pg Cabergoline in pig plasma 4 µl/min, MeOH-Water (.1% formic), C-18, 5 x 2.1 mm ng/ml Calibration curve for acrylamide using column-switching LC method with APCI-MS/MS detection ruggedness

6 Reliable High Throughput for Bioanalytical, Environmental, and Food Analyses The complex nature of the matrices associated with these samples demands selectivity, sensitivity, and robustness. The Finnigan TSQ Quantum Discovery MAX reliably delivers unmatched selectivity with H-SRM, and is unparalleled in sensitivity and robustness as a result of the Ion Max source. The benefits of the collision cell design, along with the Finnigan TSQ Quantum Discovery MAX s ability to quantify a wide range of samples with a high degree of accuracy and precision, make it unbeatable for demanding drug discovery/clinical, food and environmental analyses. For today s routine laboratory environment, the Finnigan TSQ Quantum Discovery MAX triple quadrupole mass spectrometer provides the perfect solution for these applications. Collision Cell The unique 9 collision cell design further reduces chemical noise by removing line-of-sight from the ion source to the detector. The 9 collision cell design also serves to reduce instrument foot-print, creating a conveniently sized instrument. reliability Elimination of Cross-Talk When performing LC-MS/MS analysis of multi-component mixtures by SRM, it is often necessary to use very short dwell times of 1 2 millisecond. In certain circumstances, for example in the environmental monitoring of pesticides, it is possible to get a large number of closely eluting compounds of the same chemical class, giving rise to the same product ions (see Table 1). In such assays it is possible that storage of the product ions can take place in the collision cell. In other words, fragment ions from one transition may still reside in the collision cell when the next SRM transition is monitored, resulting in cross-talk, and therefore incorrect quantification. The advanced design of the Finnigan TSQ Quantum collision cell results in the total absence of any cross-talk problems, as exemplified by the three closely eluting pesticides: triasulfuron, metasulfuron-methyl and chlorosulfuron (see Figure 1). The unique 9 collision cell

7 PESTICIDE RETENTION TIME (MIN) SRM TRANSITION (m/z) Triasulfuron > 167 Metasulfuron-methyl > 167 Chlorosulfuron > 167 Table 1: Characteristic of the three pesticides: triasulfuron, metasulfuron-methyl, and chlorosulfuron used to demonstrate zero cross-talk on the TSQ Quantum Discovery MAX Triasulfuron 42 > Metsulfuron-methyl 382 > Chlorosulfuron 358 > 167 time Metsulfuron-methyl 382 > time Chlorosulfuron 358 > 167 time Figure 1: Baseline magnified 6 =.1%. No cross-talk on the TSQ Quantum Discovery MAX Table 2: Easy development of an automated, generic, high-throughput LC/MS/MS screening method to simultaneously detect and quantify sample. As illustrated in Application Note No. 323, nearly 1 pesticides were detected with EPA tolerance

8 Finnigan TSQ Quantum Discovery MAX Column Innovations Your Perfect Partner for Quantitative LC/MS using the TSQ Quantum Discovery MAX Based on highly pure silica, Hypersil GOLD columns from Thermo provide outstanding symmetrical peaks, even when analyzing compounds that give notoriously poor peak shape on traditional silica-based chemistries. Hypersil GOLD media provides a stationary phase with C18 selectivity and a predictable elution order, but can provide new capabilities such as improved peak shape, increased peak capacity, and greater sensitivity, especially for trace compound analysis. This provides ultimate chromatographic confidence in the accuracy and quality of your LC/MS data. DASH high-throughput columns for fast separations from Thermo are designed for LC/MS applications. These short columns are ideal for ballistic gradients, providing enough retention and sensitivity for very fast LC/MS assays. The patented low dead volume design minimizes bandspreading, providing efficiencies as high as 1, plates in a 2 mm length column. Cycle times can often be two minutes or less using mm DASH columns at flow rates of µl/min. DASH columns are available in many Thermo phases, including Hypersil GOLD. Data courtesy of Leslie Romanyshyn et al; Rapid Commun Mass Spectrom. 15: (21). Copyright 21, John Wiley & Sons, Ltd. Reproduced by permission. Mobile Phase: Gradient: Detector: A: 95% H 2 O / 5% ACN B: 5% H 2 O / 95% ACN 1% B in 1.5 minutes 1% B in 1.5 minutes MS-MS Sample: 1. Glucuronide Metabolite 2. Glucuronide Metabolite 3. Parent Drug 4. Internal Standard 5. Metabolite Column: DASH BETASIL Phenyl/Hexyl, 5 µm, mm Two minute separation of drug metabolites Omeprazole MeO Lansoprazole H N N H N N O S O N CH 3 S OMe CH 3 N O CF 3 CH Mobile Phase A: 5mM NH 4 Ac at ph 9. B: ACN Gradient: 5% 1% B in 1 minutes Flow: 2 µl/min Temperature: 3º C Injection Volume: 5 µl Detection: UV 254nm Time (min) Column: Hypersil GOLD, 5 µm, mm. Sample 1: Omeprazole, Prilosec by AstraZeneca. Sample 2: Lansoprazole, Prevacid by Tap Pharma

9 Application-Specific Software Xcalibur Data System Xcalibur is the most powerful and flexible MS data handling system available for Microsoft Windows. The software provides fully-automated control of the Finnigan TSQ Quantum products and Finnigan Surveyor LC, accelerating method development with intelligent optimization tools. The functional workspace-based instrument control view creates a simplified tune, calibrate, and compound optimization environment for analytical method development. Xcalibur controls LC gradient conditions, autosampler parameters, and PDA detection data as well as the Finnigan TSQ Quantum products. All complementary and confirmatory data are acquired and displayed in a single file. Automated Data Dependent experiments are routinely performed in which the instrument repetitively records MS spectra in a chromatographic run and switches to MS/MS mode only when a component actually elutes into the mass spectrometer. Thus, the maximum amount of information is generated in a single experiment. Xcalibur s homepage start-up screen offers easy navigation through the process of Instrument Setup, Sequence Setup, and Data Acquisition. Review of results is simple with three interactive, applicationspecific browsers: Qual, Quan, and Library Browsers. Additional application-specific tools provide advanced processing functions for quantitative assays, data mining complex mixtures, and proteomic analysis. LCQUAN Software Quantitation Software for Regulated Laboratories LCQUAN has been expanded into a secure data system for quantitative analysis. The enhanced LCQUAN 2. provides the necessary resources for importing sequence information from external systems, developing methods, reviewing data, processing data, reporting data, and exporting results to external systems; all within a 21 CFR Part 11 compliant environment. LCQUAN 2. also provides multilevel security access, giving system administrators the ability to set user privileges from full system access to data review only. In addition, the software offers the ability to safely process data stored on network-based computing systems. AutoQuan Software Increased Throughput for the Drug Discovery and Quantitation Laboratory To eliminate drugs with unsuitable properties as early as possible in the drug development process, pharmacokinetic (PK) screening is getting more and more attention in early drug discovery. Due to the large number of compounds and samples that have to be analyzed at this stage, it becomes a necessity to automate the optimization of the mass spectrometer and data acquisition for each compound. AutoQuan is a software solution for the automated optimization, method development and quantitation using a triple quadrupole mass spectrometer. AutoQuan passes a sequence list to LCQUAN 2. (21 CFR Part 11 compliant software) for the acquisition and processing of bioanalytical data. Metabolite ID Metabolite Structural Identification Metabolite ID streamlines the review of drug metabolism LC-MS/MS samples. It simplifies the process of generating reconstructed ion chromatograms, mass spectra, and summary reports from hundreds of MS/MS scans isolated in a typical metabolic run. Metabolite ID harnesses the comprehensive power and flexibility within the Xcalibur software package, rapidly increasing your compound searches with an intuitive, easy-to-use interface suitable for both novice and experienced analysts. 21 CFR PART 11 LCquan 2. C O M PLIANCE

10 Laboratory Solutions Backed by Worldwide Service and Support Tap our expertise throughout the life of your instrument. As an industry leader in analytical instruments, Thermo extends its support throughout our worldwide network of Thermo-trained and certified engineers who are experts in laboratory technologies and applications. Put our team of experts to work for you in a range of disciplines, from system installation, training and technical support, to complete asset management and regulatory compliance consulting. Improve your productivity and lower the cost of instrument ownership through our product support services. Maximize uptime while eliminating the uncontrollable cost of unplanned maintenance and repairs. When it s time to enhance your systems, Thermo also offers certified parts and a range of accessories and consumables suited to your application. To learn more about our products and comprehensive service offerings, visit our Web site at In addition to these offices,thermo Electron Corporation maintains a network of representative organizations throughout the world. Australia analyze.au@thermo.com Austria analyze.at@thermo.com Belgium analyze.be@thermo.com Canada analyze.ca@thermo.com China analyze.cn@thermo.com France analyze.fr@thermo.com Germany analyze.de@thermo.com India analyze.in@thermo.com Italy analyze.it@thermo.com Japan analyze.jp@thermo.com Latin America analyze.la@thermo.com Netherlands analyze.nl@thermo.com Nordic analyze.se@thermo.com South Africa analyze.sa@thermo.com Spain analyze.es@thermo.com Switzerland analyze.ch@thermo.com UK analyze.uk@thermo.com USA analyze.us@thermo.com Thermo Finnigan LLC, San Jose, CA USA is ISO Certified. 25 Thermo Electron Corporation. All rights reserved. Prilosec a registered trademark of AstraZeneca. Prevacid are registered trademark of Tap Pharma. Microsoft and Windows are registered trademarks of Microsoft Corporation. All other trademarks are the property of Thermo Electron Corporation and its subsidiaries. Printed in the U.S.A. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. BR61677_E 1/5 S