High Throughput Screening of a Monoclonal Antibody: Practical Considerations. DDF Summit, August 2017
|
|
- William Stone
- 6 years ago
- Views:
Transcription
1 High Throughput Screening of a Monoclonal Antibody: Practical Considerations Summit, August 2017
2 Agenda 1. Background 2. Tecan liquid handler, description 3. Components (materials of construction) considerations 4. Excipient grades (compatibilities & impurities) 5. mab concentrations and solution handling 6. Lyophilized formulation capabilities 7. mab formulation screen Case study 8. Summary 9. Questions 2
3 Background What is/are the objective(s) of a high throughput screening approach to formulation development? 1) Generate a large data set 2) Derive formulation information based on the outputs from screening a relatively large number of variables 3) Make data-based decisions about formulations that will optimally stabilize a protein product. The generation of reliable data that is suitable for making a formulation decision is key. 3
4 Tecan Liquid Handler Waste/ wash 4
5 Liquids excipients, materials of construction 1. Plates, dispensing needle coatings, excipients Sugar Buffer 5
6 Plastics Component compatibilities Polystyrene Negatively charged Non-specifically binds protein Concentration dependence Polypropylene Hydrophobic Degradation, adherence Oxidation of Met, Trp groups Proteins Hydrophobic groups can adhere to plastics Problematic at low concentrations Plate Color/ Clear plastic Visual examination of contents vs light protection Plate coatings For reduction in non-specific binding Flexibility, temperature resistance, bottom geometry Polycarbonate Typically low binding 6
7 Impurity Detection Methods (in Excipients) 1. If testing is not performed for a specific heavy metal, the most common source of evidence nowadays come from a limit test being carried out. After treatment, the heavy metal is complexed with thioacetamide or precipitated as a sulphide. Then, one compares the resulting colouring of the sample solution against that from a reference lead solution. 2. These limit tests still form the majority of testing for heavy metals in the current national and international Pharmacopoeias (e.g. Ph. Eur or USP <231>). Thereby, it is possible however to make only a semiquantitative statement about the total contents of heavy metals in the sample, and in addition - and additionally, only for those heavy metals that actually form dark coloured complexes or sulphides. 3. Spectroscopic tests are to be found only in individual monographs and methods (e.g. nickel in polyols and oils, lead in sugar) to date. 4. There is no sign of any changes in the near future with regard to the methods in the Ph. Eur. and JP. This is in contrast to the US Pharmacopoeia
8 Excipients Grades and Potential issues Product Specifications TEST Meets ACS Reagent Chemical Requirements Sodium Phosphate Monobasic Monohydrate (Avantor) SPECIFICATION Trace quantities of Copper, Zinc, Nickel and Iron can cause IgG1 mab fragmentation. mabs, 2011 May-Jun; 3(3): For Laboratory,Research,or Manufacturing Use Assay (NaH₂PO₄ H₂O) ph of 5% Solution at 25 C Insoluble Matter Chloride (Cl) ACS - Sulfate (SO₄) Calcium (Ca) % <= 0.01 % <= 5 ppm <= % <= % Potassium (K) Heavy Metals (as Pb) <= 0.01 % <= % Limit Test Trace Impurities - Iron (Fe) <= % Meets Reagent Specifications for testing USP/NF monographs 8
9 Comparison of traced metal- induced fragmentation of an IgG molecule Size exclusion chromatography analysis of metal spiked samples. Specific hinge cleavage by CuII is significantly higher compared to other metals such as MgII, MnII, ZnII, FeIII and NiII under the same solvent conditions (phosphate-buffered saline at 37 C). 9
10 Other excipients Polysorbates (20 and 80) Contain peroxides and can oxidize AA side chains during heat/ light exposure. (Met, Trp Oxidation potential) Can adsorb to and react with active substance (some mabs, albumin conjugates, proteins with relatively high hydrophobicity indices). Sucrose Degradation to monomers fructose and glucose can result in glycation of proteins through the Maillard reaction. Reduction to monomer species usually occurs at acidic ph (~ph 4-5). 10
11 Tecan Lyophilization Block Uses of Lyo Block Screen protein (minimize quantity) Simultaneous screening of multiple cryo/lyo protectants Evaluate Cake characteristics Fill volumes Solids concentrations Stability Excipient: protein ratios 11
12 mab concentrations and solution handling Concentrations (10-100mg/mL) Lower concentration limit avoids surface adsorption, allows for efficient screening. Solution buffer exchanges by centrifugation methods. Higher concentration (depending on material availability) allows for evaluation of high concentration effects on stability. Solution buffer exchanges by dialysis. Tecan Filling needles Teflon coated maximize protein/ excipient transfer Ease of cleaning needle surfaces between and after dispensing Eliminates well-to-well carry over of solutions. 12
13 HTF ph and Buffer screen of a Monoclonal Antibody A case study 13
14 Study Objective Identify a target ph and buffer species that would best stabilize an IgG1 molecule against thermal degradation. Analytical tests used for evaluation Absorbance (360nm) evaluation of light scattering due to particle formation SE HPLC Purity assay - monitor monomer, aggregate and fragment cief Purity assay evaluate changes in the charge variant profile SPR Potency assay ligand binding Tests were chosen based on availability of Plate-based analytical methods 14
15 Study Design HTF screen - ph and buffer species Test article mab BDS at 100mg/mL, ph Buffer (50mM) ph test range of (0.5pH unit increments) Buffer species: Acetate, Citrate, Succinate, Histidine, Phosphate Statistical Designs (e.g. DoE) Not used (i.e. All combinations were tested.) Design expert Plate Randomization Data Analysis. 1, ) Acetate, 2) Citrate 3, 4, ) Acetate, 4) Citrate, 5)Succinate 6, 7, 8, ) Acetate, 7) Citrate, 8) Succinate, 9) Histidine 10, 11, 12, ) Citrate, 11) Succinate, 12) Histidine, 13) Phosphate 14, ) Histidine, 15) Phosphate ) Phosphate 15
16 Buffer and Sample preparation Tecan options Centrifugation (Pros and Cons) Similar to centriprep buffer exchanges but in 96-well plate format Relatively quick Buffer exchanges complete for low concentrations of proteins (~50mg/mL) Membrane fouling at high concentrations (100mg/mL) poor buffer exchange Dialysis Pros and Cons Similarities with buffer exchange processes relying on equilibration of charge species e.g TFUF Can be used for high concentration proteins (e.g. 100mg/mL) Duration (Days) 16
17 Plate design Order of addition programmed into the robotic liquid handler ph -> Std Order Random Buffer -> Acetate Citrate Acetate Citrate Succinate Acetate Citrate Succinate Histidine Citrate Succinate Histidine Phosphate Histidine Phosphate Phosphate Order Plate 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM 50mM
18 Plate prep Samples added to 96 wells (16 different buffer/ph combinations with 6 replicates) Plate sealers prevent evaporation Samples stored at 40 C for 2 weeks prior to analysis 18
19 Analytical method considerations Analytical testing was done at refrigerated conditions where possible Replicates were randomized across the plate to reduce analytical variability Samples were diluted in formulation buffer to minimize degradation during analysis Analytical methods: A320, SE-HPLC, cief, SPR 19
20 Star Plots Used for analysis of results Feature of Design Expert Star plots How to use Identify analytical methods to use If a degradant is measured the inverse of the result is used If a main peak is measured the result is used Individual results and inverse results are normalized to initial The area represented by the results are plotted in a star-design Looking for maximum area to indicate protein stability over time Advantages Allows rapid evaluation of multiple results Visual comparisons Facilitates decision-making Responses evaluated Inverse A320 SE-HPLC %monomer Inverse SE-HPLC %aggregate Inverse SE-HPLC %fragment cief %main SPR Maximum area in the star plot represented best condition for thermal stability 20
21 Star Plot results Feature of Design Expert Responses evaluated Inverse A360 SE-HPLC %monomer Inverse SE-HPLC %aggregate Inverse SE-HPLC %fragment cief %main SPR Buffer ph Combinations - Week 2 Responses AcepH5 AcepH5_5 AcepH6 CitpH5 CitpH5_5 CitpH6 CitpH6_5 HistpH6 Maximum area in the star plot represented best condition for thermal stability HistpH6_5 HistpH7 PhospH6_5 PhospH7 PhospH7_5 SucpH5_5 SucpH6 SucpH6_5 Legend A320 Biacore SEC Agg SEC Frag SEC Main cief Main 21
22 Gibbs Donnan effects At high concentrations, chemical potential and Gibbs- Donnan effect play a role in sample preparation. Acetate ph 5.0 was closer to ph 5.5 when measured Acetate ph 5.5 was closer to ph 6.0 when measured Additional studies were performed with direct buffer exchanges to confirm acetate results. Results confirmed. Message: At high protein concentrations, and depending on target ph, protein self-buffering effects modify the efficiency of buffer exchanges. Must be taken into account sample prep. 22
23 Summary 1. Tecan liquid handler, - described the layout, liquid handling and plate characteristics 2. Components (materials of construction) considerations adherence to plastics, compatibility 3. Excipient grades (compatibilities & impurities) Potential metal impurities and excipient degradation issues 4. mab concentrations and solution handling working concentration range of tecan 5. Lyophilized formulation capabilities Lyo block characteristics 6. mab formulation screen Case study 23
24 Acknowledgements Biopharmaceutical Product Sciences and BioAnalytical Sciences, Statistical Sciences, R&D GSK Melissa Shuman Jim Ludlow Nicole Lundberg Andrea Frangiosa Laurie Pompeo Jennifer Woods Harnath Doddapenani Becky Wood Jennifer Dally Tom Wrzosek Conference organizers Thank you for your attention 24
25 Questions
Highly automated protein formulation development: a case study
Application Note Highly automated protein formulation development: a case study Introduction and background Therapeutic proteins can be inherently prone to degradation and instability, and they often pose
More informationPhysisorption of Antibodies using BioReady Bare Nanoparticles
TECHNICAL RESOURCE Lateral Flow Immunoassays Physisorption of Antibodies using BioReady Bare Nanoparticles Introduction For more than 20 years, lateral flow immunoassay diagnostic tests have provided a
More informationMeet Stunner: The one-shot protein concentration and sizing combo
TECH NOTE Meet Stunner: The one-shot protein concentration and sizing combo Introduction What if you could get a better read on the quality of your biologics and use less sample at the same time? Stunner
More informationPEG / Ion. User Guide HR2-139 (pg 1) glycol 3,350. Refer to the enclosed PEG/Ion HT reagent formulation for additional information on all 96 reagents.
PEG / Ion TM User Guide HR2-139 (pg 1) Applications Crystallization screen for soluble biological macromolecules. Features Reagent formulation developed at Hampton Research Screens a profile of anions,
More informationPreview Slides for Introduction to Protein Formulation and Delivery
Preview Slides for Introduction to Protein Formulation and Delivery Tim Kelly, Ph.D. VP, Biopharmaceutical Development KBI Biopharma, Inc Durham, NC Pooja Arora, Ph.D. Senior Manufacturing Technical Specialist
More informationCation Exchange HPLC Columns
Cation Exchange HPLC Columns Hamilton offers seven polymeric packing materials for cation exchange separations. Type Recommended Application(s) PRP-X00 PRP-X00 PRP-X800 HC-0 HC-7 Ca + HC-7 H + HC-7 Pb
More informationHPLC Winter Webinars Part 2: Sample Preparation for HPLC
HPLC Winter Webinars Part 2: Sample Preparation for HPLC Jon Bardsley, Application Chemist Thermo Fisher Scientific, Runcorn/UK The world leader in serving science What am I Going to Talk About? What do
More informationPCBs Lower Chlorinated ELISA Kit
PCBs Lower Chlorinated ELISA Kit Cat. No.:DEIA6835 Pkg.Size:100T Intended use The PCBs Lower Chlorinated ELISA Kit is an immunoassay for the quantitative and sensitive detection of Biphenyls-Lower Chlorinated
More informationClemens Stilling Characterisation, Analytical Sciences for Biologicals, UCB Pharma S.A. Alun, living with Parkinson s disease
Clemens Stilling Characterisation, Analytical Sciences for Biologicals, UCB Pharma S.A. Alun, living with Parkinson s disease Outline Short intro Aggregate characterisation during candidate selection and
More informationIntroduction to Pharmaceutical Chemical Analysis
Introduction to Pharmaceutical Chemical Analysis Hansen, Steen ISBN-13: 9780470661222 Table of Contents Preface xv 1 Introduction to Pharmaceutical Analysis 1 1.1 Applications and Definitions 1 1.2 The
More informationProtein separation and characterization
Address:800 S Wineville Avenue, Ontario, CA 91761,USA Website:www.aladdin-e.com Email USA: tech@aladdin-e.com Email EU: eutech@aladdin-e.com Email Asia Pacific: cntech@aladdin-e.com Protein separation
More informationTaKaRa BCA Protein Assay Kit
Cat. # T9300A For Research Use TaKaRa BCA Protein Assay Kit Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Storage... 3 IV. Materials Required by not Provided... 3 V. Precautions
More informationBis sulfone Reagents. Figure 1.
Bis sulfone Reagents An intact IgG molecule has four accessible inter chain disulfide bonds that can be reduced to form eight free cysteine thiols, which can serve as sites for conjugation. The reaction
More informationHigh Sensitivity Polyethylene Glycol (PEG) ELISA Kit
High Sensitivity Polyethylene Glycol (PEG) ELISA Kit Cat. No.:DEIA6158 Pkg.Size:96T Intended use High Sensitivity Polyethylene Glycol (PEG) ELISA Kit is High Sensitivity ELISA for the Measurement of PEG
More informationCONFOCHECK. Innovation with Integrity. Infrared Protein Analysis FT-IR
CONFOCHECK Infrared Protein Analysis Innovation with Integrity FT-IR CONFOCHECK: FT-IR System for Protein Analytics FT-IR Protein Analysis Infrared spectroscopy measures molecular vibrations due to the
More informationChemistry 20 Final Review Solutions Checklist Knowledge Key Terms Solutions
Chemistry 20 Final Review Solutions Checklist Have you mastered the concepts, applications, and skills associated with the following items? Check them off when you are confident in your understanding.
More informationCrystal ScreenTM. User Guide HR2-110 (pg 1)
Crystal ScreenTM User Guide HR2-110 (pg 1) Crystal Screen is a complete reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules. Crystal Screen is
More informationBRIEFING. Pharmacopeial Discussion Group Sign Off Document Attributes EP JP USP Definition Loss on drying Readily carbonizable substances
BRIEFING Saccharin, NF 22 page 2825 and page 1711 of PF 29(5) [Sept. Oct. 2003]. The United States Pharmacopeia is the coordinating pharmacopeia for the international harmonization of the compendial standards
More informationAppendix: 1. Sodium bicarbonate 0.84 gm (10 mm/l) 50ml of 2% sodium carbonate in 0.10N sodium hydroxide
Appendix: 1 Chemicals, Reagents and Buffers 1. BUFFERS FOR WBC EXTRACTION WBC lysis buffer (for 1 liter) Ammonium chloride 8.3 gm (150 mm/l) Sodium bicarbonate 0.84 gm (10 mm/l) 1 X reagent EDTA 29 mg
More informationGyrolab Protein A Kit Quantification of residual Protein A ligands in the presence of excess amounts of IgG
Quantification of residual Protein A ligands in the presence of excess amounts of IgG Product number P0020456 P0020457 Product Name Gyrolab Protein A Kit for MabSelect SuRe Ligand Gyrolab Protein A Kit
More informationCrystal Screen CryoTM Solutions for Crystal Growth
User Guide HR2-122 (pg 1) Crystal Screen Cryo is a complete sparse matrix reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules in the presence of
More informationHuman Coagulation Factor X Total Antigen ELISA Kit
Human Coagulation Factor X Total Antigen ELISA Kit Catalog No: IHFXKT-TOT Lot No: SAMPLE INTENDED USE This human coagulation Factor X antigen assay is intended for the quantitative determination of total
More informationCu-Creatinine- Metol system
Quantification of Creatinine in Human Serum using Metol as a Chromogenic Probe Materials and methods 6.1. Reagents 6.1.1. N-methyl-p-aminophenol sulfate N-methyl-p-aminophenol sulfate also denoted as Metol
More informationMeet Our Uncle: 12 Stability Applications on One Platform
Tech Note Meet Our Uncle: 12 Stability Applications on One Platform Uncle is an all-in-one stability platform that enables twelve different applications with one instrument. Fluorescence, static light
More informationIsothermal experiments characterize time-dependent aggregation and unfolding
1 Energy Isothermal experiments characterize time-dependent aggregation and unfolding Technical ote Introduction Kinetic measurements have, for decades, given protein scientists insight into the mechanisms
More informationPEGRx. User Guide HR2-086 (pg 1)
User Guide HR2-086 (pg 1) Applications Crystallization screen for biological macromolecules. Features Reagent formulation developed at. Screens an array of polymers of varying molecular weight in a low
More informationChemical Reactions and Equations
Chemical Reactions and Equations Question 1: Why should a magnesium ribbon be cleaned before burning in air? Magnesium is very reactive metal. When stored it reacts with oxygen to form a layer magnesium
More informationAdsorption of Amino Acids, Aspartic Acid and Lysine onto Iron-Oxide Nanoparticles
Supporting Information Adsorption of Amino Acids, Aspartic Acid and Lysine onto Iron-Oxide Nanoparticles Klementina Pušnik, Mojca Peterlin, Irena Kralj Cigić, Gregor Marolt, Ksenija Kogej, Alenka Mertelj,
More informationIgG (Rabbit) ELISA Kit
IgG (Rabbit) ELISA Kit Catalog Number KA2017 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationMethods of purification
Methods of purification Question Paper 1 Level IGSE Subject hemistry (0620/0971) Exam oard ambridge International Examinations (IE) Topic Experimental techniques Sub-Topic Methods of purification ooklet
More information10.3 Types of Chemical Reactions
10.3 Types of Chemical Reactions Chemical equations follow certain patterns Combination (synthesis ) The number of possible chemical is very large. However, there are a limited number of structural patterns
More informationAdvantages of Agilent AdvanceBio SEC Columns for Biopharmaceutical Analysis
Advantages of Agilent AdvanceBio SEC Columns for Biopharmaceutical Analysis Comparing Columns from Different Vendors to Improve Data Quality Technical Overview Introduction Size exclusion chromatography
More informationPrealbumin (Mouse) ELISA KitI
Prealbumin (Mouse) ELISA KitI Catalog Number KA2070 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of
More informationFor the detection and quantitation of glyphosate in water (groundwater, surface water, well water).
Glyphosate ELISA Kit Cat.No: DEIA6865 Lot. No. (See product label) Size 96T Intended Use For the detection and quantitation of glyphosate in water (groundwater, surface water, well water). Principle Of
More informationPolyethylene Glycol (PEG), High Sensitive ELISA
K-ASSAY Polyethylene Glycol (PEG), High Sensitive ELISA For the high sensitive quantitative determination of PEG and PEGylated proteins in serum or plasma Cat. No. KT-657 For Research Use Only. 1 K-ASSAY
More informationGCE O' LEVEL PURE CHEMISTRY (5073/02) Suggested Answers for 2016 O Level Pure Chemistry Paper 2
Section A (50 M) Aa) trend The number of electron shell increases The number of valence electrons increases Proton number increases There is a change in character from metallic to non-metallic Only true
More informationThe Human IgD ELISA KIT is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgD in Human Biological Samples.
Human IgD ELISA KIT Cat. No.:DEIA7956 Pkg.Size:96T Intended use The Human IgD ELISA KIT is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgD in Human Biological Samples. General
More informationBisphenol A ELISA Kit
Bisphenol A ELISA Kit Catalog Number KA1495 96 assays Version: 11 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationIgA (Guinea Pig) ELISA Kit
IgA (Guinea Pig) ELISA Kit Catalog Number KA2032 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationUBI. SCoV EIA DIRECTION MANUAL FOR IN VITRO DIAGNOSTIC RESEARCH USE ONLY CONTENTS
UBI SCoV EIA DIRECTION MANUAL FOR IN VITRO DIAGNOSTIC RESEARCH USE ONLY CONTENTS TRADE NAME & INTENDED USE SUMMARY AND EXPLANATION OF THE TEST CHEMICAL AND BIOLOGICAL PRINCIPLES OF THE PROCEDURE REAGENT
More informationRat Advanced Glycation End Products(AGEs) ELISA Kit
Rat Advanced Glycation End Products(AGEs) ELISA Kit Catalog No. CSB-E09413r (96 T) This immunoassay kit allows for the in vitro quantitative determination of rat AGEs concentrations in serum, plasma and
More informationCANINE COMPLEMENT FACTOR 3 (C3) ELISA
CANINE COMPLEMENT FACTOR 3 (C3) ELISA For the quantitative determination of Complement Factor 3 in canine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number:
More informationBCA Protein Macro Assay Kit
INSTRUCTION MANUAL BCA Protein Macro Assay Kit Kit for Protein Quantification (Cat. No. 39228) Contents 1. BCA PROTEIN MACRO ASSAY KIT 2 1.1. General information 2 1.2. Kit components 2 1.3. Additionally
More informationA New On-line Cyanide Analyzer for Measurement of Cyanide in Hydrometallurgical Processing of Precious Metal Ores
Application Note 37890312 A New On-line Cyanide Analyzer for Measurement of Cyanide in Hydrometallurgical Processing of Precious Metal Ores Keywords CNSolution 9310 On-line Cyanide Analyzer Cyanidation
More informationNon-Interfering Protein Assay Kit Cat. No
Visit our interactive pathways at /pathways User Protocol 488250 Rev. 5 January 2006 RFH Page 1 of 1 Non-Interfering Protein Assay Kit Cat. No. 488250 Note that this user protocol is not lot-specific and
More informationIgE (Dog) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only.
IgE (Dog) ELISA Kit Catalog Number KA1937 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General Information...
More informationDual-Range TM BCA Protein Assay Kit
Manual DualRange TM BCA Protein Assay Kit BC31/BC325/BC35 V3.1 For Research Use Only Introduction DualRange TM BCA Protein Assay Kit is a perfect protein assay method. It based on bicinchoninic acid (BCA)
More informationExam I Answer Key: Summer 2006, Semester C
1. Which of the following tripeptides would migrate most rapidly towards the negative electrode if electrophoresis is carried out at ph 3.0? a. gly-gly-gly b. glu-glu-asp c. lys-glu-lys d. val-asn-lys
More informationDETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
CHAPTER 9 DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 9 Determination of drug release during
More informationHuman Coagulation Factor XII Total Antigen ELISA Kit
Human Coagulation Factor XII Total Antigen ELISA Kit Catalog No: IHFXIIKT-TOT Lot No: SAMPLE INTENDED USE This human coagulation Factor XII antigen assay is intended for the quantitative determination
More informationCA125 (Human) ELISA Kit
CA125 (Human) ELISA Kit Catalog Number KA0205 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationAssay procedure for. PeliKine compact TM ELISA kit (288 tests) Research Use Only. Sanquin Reagents
Assay procedure for PeliKine compact TM ELISA kit (288 tests) Research Use Only Sanquin Reagents Plesmanlaan 125 1066 CX Amsterdam The Netherlands reagents@sanquin.nl www.sanquinreagents.com For The Netherlands
More informationClearing the Confusion: GPC, SEC, GFC What, When, Why, and How?
Clearing the Confusion: GPC, SEC, GFC What, When, Why, and How? Jean Lane Applications Engineer LC Columns & Consumables Technical Support January 19, 2017 What we will cover What - Clarification of the
More informationMonkey Kidney injury molecule 1,Kim-1 ELISA Kit
Monkey Kidney injury molecule 1,Kim-1 ELISA Kit Catalog No: E0785Mo 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH
More informationMouse Cholecystokinin (CCK) ELISA Kit
Mouse Cholecystokinin (CCK) ELISA Kit Catalog No. CSB-E08115m (96T) This immunoassay kit allows for the in vitro quantitative determination of mouse CCK concentrations in serum, plasma and other biological
More informationC3 (Mouse) ELISA Kit. Catalog Number KA assays Version: 15. Intended for research use only.
C3 (Mouse) ELISA Kit Catalog Number KA1926 96 assays Version: 15 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationMicrocystin-LR ELISA Kit
Microcystin-LR ELISA Kit Catalog Number KA1496 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationProPac WCX-10 Columns
ProPac WCX-10 Columns Guidance for column use Tips to maximize column lifetime ProPac WCX-10 Column Tips and Tricks This guide provides essential information and invaluable guidelines for mobile phases,
More informationClass X. Exercises solution
Exercises solution Question 1: Which of the statements about the reaction below are incorrect? Lead is getting reduced. Carbon dioxide is getting oxidised. Carbon is getting oxidised. Lead oxide is getting
More informationIgG (Canine) ELISA. Please see Appendix A for Reference Serum information. For Research Use Only. Not For Use In Diagnostic Procedures.
IgG (Canine) ELISA For the quantitative determination of IgG in canine serum or plasma Please see Appendix A for Reference Serum information. For Research Use Only. Not For Use In Diagnostic Procedures.
More informationUnit B Analysis Questions
Unit B Analysis Questions ACTIVITY 12 1. What two types of information do you think are the most important in deciding which material to use to make drink containers? Explain. 2. What additional information
More informationIgG (Cat) ELISA Kit. Catalog Number KA assay Version: 01. Intend for research use only.
- IgG (Cat) ELISA Kit Catalog Number KA2048 96 assay Version: 01 Intend for research use only www.abnova.com Introduction and Background A. Test principle The principle of the double antibody sandwich
More informationHUMAN MYELOPEROXIDASE
HUMAN MYELOPEROXIDASE Immunoperoxidase Assay for Determination of Myeloperoxidase in Human Samples DIRECTIONS FOR USE Version3 GB0073 For Research Use Only, NOT for Diagnostic Purposes Please Read this
More informationSphere Scientific Corporation MonoPS Microspheres Our standard polystyrene microspheres are extremely uniform with an excellent lot-to-lot reproducibility. Those microspheres are mainly devoted to hydrophobic
More informationChapter 2. The Chemistry of Life
Chapter 2 The Chemistry of Life Introduction Cells, tissues and organs composed of chemicals Chemical reactions important for function Chemistry is the study of elements, compounds, chemical reactions,
More informationUNDECAGOLD PRODUCT INFORMATION MONOMALEIMIDO UNDECAGOLD LABELING REAGENT
UNDECAGOLD 95 Horse Block Road, Yaphank NY 11980-9710 Tel: (877) 447-6266 (Toll-Free in US) or (631) 205-9490 Fax: (631) 205-9493 Tech Support: (631) 205-9492 tech@nanoprobes.com www.nanoprobes.com PRODUCT
More informationProtein A ELISA Kit. For the detection of MabSelect SuRe ligand. User Guide P/N Part Number rev Customer Service (800)
Protein A ELISA Kit For the detection of MabSelect SuRe ligand User Guide P/N 9333-1 Part Number 9333-1 rev.100831 Customer Service (800) 622-2259 Disclaimer This ELISA kit is intended FOR RESEARCH USE
More informationDRG International, Inc., USA Fax: (908)
Please use only the valid version of the package insert provided with the kit. This kit is intended for Research Use Only. Not for use in diagnostic procedures. 1 INTENDED USE Competitive immunoenzymatic
More informationNanoliter Scale High-Throughput Protein Crystallography Screening with the Echo Liquid Handler
TM Application Note P100 Echo Liquid Handler Nanoliter Scale High-Throughput Protein Crystallography Screening with the Echo Liquid Handler Bonnie Edwards, David Harris Labcyte Inc. Abstract The Echo liquid
More informationCYCLOSERINE Final text for addition to The International Pharmacopoeia. (November 2008) CYCLOSERINUM CYCLOSERINE
December 2008 CYCLOSERINE Final text for addition to The International Pharmacopoeia (November 2008) This monograph was adopted at the Forty-third WHO Expert Committee on Specifications for Pharmaceutical
More informationHuman anti-ganglioside IgG antibody (GM1-IgG) ELISA Kit
Human anti-ganglioside IgG antibody (GM1-IgG) ELISA Kit Catalog No. CSB-E09694h (96 tests) This immunoassay kit allows for the in vitro semi-quantitative determination of human GM1-IgG concentrations in
More informationMole Conversions Worksheet
Mole Conversions Worksheet There are three mole equalities. They are: 1 mol = 6.02 x 10 particles 1 mol = g-formula-mass (periodic table) 1 mol = 22.4 L for a gas at STP Each equality can be written as
More informationRat Prolactin ELISA Kit
Rat Prolactin ELISA Kit Catalog No: IRPRLKT Lot No: SAMPLE INTENDED USE This rat prolactin antigen assay is intended for the quantitative determination of prolactin antigen in rat plasma. For research
More informationMouse Retinol Binding Protein 4 ELISA
Mouse Retinol Binding Protein 4 ELISA For the quantitative determination of RBP4 in mouse serum and plasma. Please see Appendix A for Reference Serum Information. For Research Use Only. Not For Use In
More informationMicrocystin-LR ELISA Kit
Microcystin-LR ELISA Kit Catalog Number KA1496 96 assays Version: 10 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationWebsite: Page 1. Page 14»Exercise» Page 15» Question 1:
Page 14»Exercise» Question 1: Which of the statements about the reaction below are incorrect? (a) Lead is getting reduced. (b) Carbon dioxide is getting oxidised. (c) Carbon is getting oxidised. (d) Lead
More informationMouse IgE ELISA. Cat. No. KT-401 K-ASSAY. For the quantitative determination of IgE in mouse biological samples. For Research Use Only.
K-ASSAY Mouse IgE ELISA For the quantitative determination of IgE in mouse biological samples Cat. No. KT-401 For Research Use Only. 1 Rev. 13328401 K-ASSAY PRODUCT INFORMATION Mouse IgE ELISA Cat. No.
More informationMixtures 1 of 38 Boardworks Ltd 2016
Mixtures 1 of 38 Boardworks Ltd 2016 Mixtures 2 of 38 Boardworks Ltd 2016 Pure and impure substances 3 of 38 Boardworks Ltd 2016 All materials can be classified as either a pure substance or an impure
More informationCasein (Bovine) ELISA Kit
Casein (Bovine) ELISA Kit Catalog Number KA1955 96 assays Version: 01 Intended for research use only www.abnova.com Introduction and Background A. Test principle The principle of the double antibody sandwich
More informationPorcine Factor-related Apoptosis(FAS) ELISA Kit
Porcine Factor-related Apoptosis(FAS) ELISA Kit Catalog No. CSB-E09369p (96T) This immunoassay kit allows for the in vitro quantitative determination of porcine FAS concentrations in serum, plasma and
More informationSelf-assembly of PEGylated Gold Nanoparticles. with Satellite Structures as Seeds
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 216 Electronic Supplementary Information for Self-assembly of PEGylated Gold Nanoparticles with Satellite
More informationHuman papillomavirus,hpv ELISA Kit
Human papillomavirus,hpv ELISA Kit Catalog No: E0787h 96 Tests Operating instructions www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE
More informationMouse Transferrin ELISA
K-ASSAY Mouse Transferrin ELISA For the quantitative determination of Transferrin in mouse serum or plasma Cat. No. KT-348 For Research Use Only. 1 Rev. 041907 K-ASSAY PRODUCT INFORMATION Mouse Transferrin
More informationGlossary of Common Laboratory Terms
Accuracy A measure of how close a measured value is to the true value. Assessed by means of percent recovery of spikes and standards. Aerobic Atmospheric or dissolved oxygen is available. Aliquot A measured
More informationIgE (Rat) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only.
IgE (Rat) ELISA Kit Catalog Number KA1951 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General Information...
More informationShedding a Light on Solubility Measurement and Prediction Solubility Sub Team's Story to Date
Shedding a Light on Solubility Measurement and Prediction Solubility Sub Team's Story to Date Slide 1 Outline Why is organic solubility important? Organisation of the group HTE working group Prediction
More informationIgG (Chicken) ELISA Kit
IgG (Chicken) ELISA Kit Catalog Number KA2426 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the
More informationDetermination of trace anions in concentrated hydrofluoric acid
APPLICATION NOTE 78 Determination of trace anions in concentrated hydrofluoric acid Authors Archava Siriraks Thermo Fisher Scientific, Sunnyvale, CA Keywords HF, ICS-5000 +, IonPac AS10, IonPac AC10, ion
More informationC hapter ATOMS. (c) (ii) and (iii) (d) (ii) and (iv)
C hapter 3 ATOMS AND MOLECULES 1. Which of the following correctly represents 360 g of water? (i) 2 moles of H 2 0 (ii) 20 moles of water (iii) 6.022 10 23 molecules of water (iv) 1.2044 10 25 molecules
More informationTF (Bovine) ELISA Kit
TF (Bovine) ELISA Kit Catalog Number KA2047 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationBRIEFING. (EXC: K. Moore.) RTS C Propylparaben C 10 H 12 O Benzoic acid, 4 hydroxy, propyl ester; Propyl p hydroxybenzoate [ ].
BRIEFING Propylparaben. The European Pharmacopoeia is the coordinating pharmacopeia for the international harmonization of the compendial standards for the Propylparaben monograph, as part of the process
More informationMETHOD 3010A ACID DIGESTION OF AQUEOUS SAMPLES AND EXTRACTS FOR TOTAL METALS FOR ANALYSIS BY FLAA OR ICP SPECTROSCOPY
METHOD 3010A ACID DIGESTION OF AQUEOUS SAMPLES AND EXTRACTS FOR TOTAL METALS FOR ANALYSIS BY FLAA OR ICP SPECTROSCOPY 1.0 SCOPE AND APPLICATION 1.1 This digestion procedure is used for the preparation
More informationHuman Anti-Ovary Antibody (IgG)ELISA Kit
Human Anti-Ovary Antibody (IgG)ELISA Kit Catalog No. MBS703636 (96 tests) This immunoassay kit allows for the in vitro semi-quantitative determination of human Anti-Ovary Antibody(IgG) concentrations in
More informationSpherotech, Inc Irma Lee Circle, Unit 101, Lake Forest, Illinois PARTICLE COATING PROCEDURES
SPHERO TM Technical Note STN-1 Rev C. 041106 Introduction Currently, there are several methods of attaching biological ligands to polystyrene particles. These methods include adsorption to plain polystyrene
More informationChapter 4. Aqueous Reactions and Solution Stoichiometry
Sample Exercise 4.1 (p. 127) The diagram below represents an aqueous solution of one of the following compounds: MgCl 2, KCl, or K 2 SO 4. Which solution does it best represent? Practice Exercise 1 (4.1)
More informationHuman placenta lactogen (HPL)ELISA Kit. MyBioSource.com. Catalog No. MBS (96T)
\ Human placenta lactogen (HPL)ELISA Kit Catalog No. MBS703864 (96T) This immunoassay kit allows for the in vitro quantitative determination of human HPL concentrations in serum, plasma. Expiration date
More informationExplain freezing-point depression and boiling-point elevation at the molecular level.
Solutions 1 UNIT4: SOLUTIONS All important vocabulary is in Italics and bold. Describe and give examples of various types of solutions. Include: suspension, emulsion, colloid, alloy, solute, solvent, soluble,
More informationab83360 Ammonia Assay Kit
Version 8 Last updated 18 December 2017 ab83360 Ammonia Assay Kit For the measurement of total ammonia and ammonium levels in various samples This product is for research use only and is not intended for
More informationRUBIC Buffer Screen For stable, happy proteins From purification all the way through to characterization by NMR, SAXS or Crystallography.
RUBIC Buffer Screen MD1-96 For stable, happy proteins From purification all the way through to characterization by NMR, SAXS or Crystallography. RUBIC Buffer Screen- designed at the EMBL Hamburg and optimized
More informationIgA ELISA. For the quantitative determination of IgA in human serum and plasma. For Research Use Only. Not for Use in Diagnostic Procedures.
IgA ELISA For the quantitative determination of IgA in human serum and plasma For Research Use Only. Not for Use in Diagnostic Procedures. Catalog Number: 41-IGAHU-E01 Size: 96 Wells Version: 3 L61-19-ALPCO
More information