C18, C18-WP, HFC18-16, HFC18-30,RP-AQUA, C8, PFP,

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1 C8, C8-WP, HFC8-, HFC8-,RP-AQUA, C8, PFP, Phenyl, HILIC-Amide and -EP HPLC column SunShell Core Shell Particle ChromaNik Technologies Inc.

2 Back pressure, MPa HETP, mm SunShell is a core shell silica column made by ChromaNIk Technologies. The next generation to Core Shell particle Features of SunShell *. mm of core and. mm of superficially porous silica layer *Same efficiency and high throughput as a Sub mm particle *Same pressure as a mm particle (less than a half then a sub mm particle) *Same chemistry as Sunniest technology (reference figure ) *Good peak shape for all compounds such as basic, acidic and chelating compounds *High stability ( ph range for SunShell C8,. to ) * Low breeding. mm. mm Core. mm. mm Core Porous silica Schematic diagram of a core shell silica particle. mm Superficially porous silica H H Final TMS H Schematic diagram of bonding of SunShell C8 Van Deemter Equation A term : Eddy diffusion(dp is particle diameter) B term : Longitudinal diffusion (Dm is diffusion coefficient) C term : Mass transfer 8 Fully porous um Fully porous um Fully proous.8 um SunShell. um H B term A term Van Deemter plot u C term 8 Fully porous. um Column: C8, x. mm C8 Acetonitrile/water=(/) Temperature: o C Sample : Naphthalene Mobile phase velocity, mm/sec Comparison of plate height plots.. Flow rate, ml/min Sunniest C8-HT. um Brand A C8.9 um Brand B C8.8 um Brand C C8. um Brand D C8. um SunShell C8. um Column dimension: x. mm Acetonitrile/water=(/) Temperature: o C Comparison of back pressure for high throughput columns SunShell C8 shows same efficiency as a sub mm C8. In comparison between fully porous. mm and core shell. mm (SunShell), SunShell shows lower values for A term, B term and C term of Van Deemter equation. The core shell structure leads higher performance to compare with the fully porous structure. Furthermore back pressure of SunShell C8 is less than a half to compare with sub- mm C8s.

3 Plate height (μm) Why does a. mm core shell particle show the same performance as a sub mm particle? All terms in Van Deemter Equation reduce. A term Company F, mm D :. mm D :.9 mm D 9 :. mm D 9 /D =.9 Sunniest, mm D :. mm D :. mm D 9 :. mm D 9 /D =. SunShell,. mm D :. mm D :. mm D 9 :.8 mm D 9 /D =. Comparison of Particle ze Distribution Wide particle distribution (Conventional silica gel D 9 /D =.) Flow of mobile phase Narrow particle distribution (core shell silica D 9 /D =.) Packing state of core shell and fully porous silica The size distribution of a core shell (SunShell) particle is much narrower than that of a conventional totally porous particle, so that the space among particles in the column reduces and efficiency increases by reducing Eddy Diffusion (multi-path diffusion) as the A term in Van Deemter Equation. Diffusion of a solute is blocked by the existence of a core, so that a solute diffuses less in a core shell silica column than in a totally porous silica column. Consequently B term in Van Deemter Equation reduces in the core shell silica column. B term Totally porous silica Core shell silica A solute diffuses in a pore as well as outside of particles. A core without pores blocks diffusion of a solute. Difference of longitudinal diffusion 8 Column: SunShell C8,. mm x. mm Totally porous mm x. mm Acetonitrile/water=(/) Sample : Naphthalene degree C Core shell. um degree C Core shell. um degree C Totally porous um....8 Flow rate (ml/min) Plot of Flow rate and Plates height C term Comparison of diffusion path As shown in the left figure, a core shell particle has a core so that the diffusion path of samples shortens and mass transfer becomes fast. This means that the C term in Van Deemter Equation reduces. In other words, HETP (theoretical plate) is kept even if flow rate increases. A. mm core shell particle shows as same column efficiency as a totally porous sub- mm particle. The right figure shows that a diffusion width of a sample in a. mm core shell particle and a mm totally porous particle. Both diffusion widths are almost same. The. mm core shell particle is superficially porous, so that the diffusion width becomes narrower than particle size. Same diffusion means same efficiency. Diffusion Ca.. mm sample Core Shell. mm Sample emanates in a particle after it enters. Diffusion. mm sample Totally porous. mm. mm Diffusion of sample in core shell and totally porous silica Comparison of Performance by Plate/Pressure Plate Back press. (MPa) Plate/back press. Sunniest C8 HT. mm 9,9. 9 Brand A C8.9 mm,. Brand B C8.8 mm, 9. Brand C C8. mm,. 8 SunShell C8. mm 9, Sunniest C8 HT. mm Brand A C8.9 mm Brand B C8.8 mm Brand C C8. mm SunShell C8. mm,, Column: x. mm C8, Acetonitrile/water=(/), Temperature: o C Under a constant back pressure condition, SunShell C8 showed more than times higher performance to compare with totally sub-mm porous C8s.

4 Characteristics of SunShell C8 Particle size Core shell silica Pore diameter Specific surface area Carbon content Bonded phase USP L# C8 End-capping Maximum operating pressure Available ph range SunShell C8. mm 9 nm m /g % C8 L Sunniest End-capping MPa or 8, psi. - k=. k=. Company A C8. MPa k=9. k=9. SunShell C8 Comparison of standard samples between core shell C8s Company B C8. MPa Company C C8. MPa Company D C8. MPa Column: Company A C8, x. mm (. MPa) Company B C8, x. mm (. MPa) Company C C8, x. mm (. MPa) Company D C8, x. mm (. MPa) SunShell C8, x. mm (.8 MPa) CH H/H =/ Flow rate:. ml/min, Temperature: ºC Sample: = Uracil, = Caffeine, = Phenol, = Butylbenzene = o-terphenyl, = Amylbenzene, = Triphenylene Hydrogen bonding (Caffeine/Phenol) Hydrophobicity (Amylbenzene/Butylbenzene) Steric selectivity (Triphenylene/o-Terphenyl) Company A C8.8.. SunShell C8.8 MPa k=. Company B C8... Company C C8... Company D C SunShell C8.9.. Retention of standard samples and back pressure were compared for five kinds of core shell type C8s. Company A C8 showed only a half retention to compare with SunShell C8. Steric selectivity becomes large when ligand density on the surface is high. SunShell C8 has the largest steric selectivity so that it has the highest ligand density. This leads the longest retention time. Comparison of pyridine Comparison of xine Comparison of formic acid Company A C8 TF=. Company A C8 TF=. Company A C8 Company B C8 TF=.9 Company B C8 TF=.8 Company B C8 Company C C8 Company D C8 TF=. TF=. Company C C8 Company D C8 TF=. TF=. Company C C8 Company D C8 SunShell C8 TF=. SunShell C8 TF=. SunShell C8 Column dimension: x. mm CH H/H =/ Flow rate:. ml/min Temperature: ºC Detection: UV@nm Sample: = Uracil = Pyridine = Phenol 8 9 Column dimension: x. mm CH CN/mM H P =/9 Flow rate:. ml/min Temperature: ºC Detection: UV@nm Sample: = 8-Quinolinol (xine) = Caffeine 8 9 Column dimension: x. mm CH CN/.% H P =/98 Flow rate:. ml/min Temperature: ºC Detection: UV@nm Sample: = Formic acid = Acetic acid = Propionic Acid Residual silanol groups make pyridine be tailing under methanol/water mobile phase condition. SunShell C8 shows a sharp peak for pyridine. 8-Quinolinol (xine) is a metal chelating compound. Metal impurities in the core shell particle leads the tailing for oxine peak. Formic acid is used as an indicator for a acidic inertness. SunShell and Company A and C C8 show a sharp peak.

5 USP tailing factor Theoretical plate Theoretical plate Loading capacity of amitriptyline as a basic compound Amitriptyline overlords much more at acetonitrile/buffer mobile phase than methanol/buffer. Three kinds of core shell C8s were compared loading capacity of amitriptyline at three different mobile phases. Common condition: Column dimension, x. mm, flow rate;. ml/min, temperature; o C Acetonitrile/mM phosphate buffer ph.=(:) Acetonitrile/mM acetate ammonium ph.8=(:) 8 SunShell C8 Company C C8 Sunniest C8 um Company A C8 Company B C8 Company D C8 倍 8 Sunshell C8 Company A C8 Company P C8 Company T C8 Company S C8 倍... Sample weight/μg TF=.8 TF=. 8 9 TF=. TF=. TF=. SunShell C8 C C8 = Amitriptyline (.mg) Sunniest C8 μm (fully porous) A C8 B C8 TF=.8 Sample: D C8 = Uracil (.mg) = Propranolol (.mg) = Nortriptyline (.mg)... Sample weight/μg TF=. TF=.89 TF=. TF=. TF=. 8 9 SunShell C8 C C8 A C8 B C8 D C8 Theoretical plate was calculated by σ method using peak width at.% of peak height..% Acetonitrile/.% formic acid=(:)... Company D C8 Company B C8 Company A C8 Company C C8 SunShell C8 倍 USP Tailing factor Comparison column. Kinetex C8,. μm. Accucore C8,. μm. PoroShell C8 EC,. μm. Ascentis Express C8,. μm. SunShell C8,. μm.... Sample weight/μg Amitriptyline overloads at low weight when acetonitrile/.% formic acid mobile phase. A peak is shifted forward under overloading. All columns are core shell type. All columns sized x. mm show 8, to, plates for a neutral compound. However regarding a basic compound like amitriptyline, SunShell C8 and company C C8 showed a good peak, while Company A, B and D C8 showed a poor peak. Company A C8 overloaded at more than. mg of amitriptyline while SunShell C8 overloaded at more than from. to mg of amitriptyline. Surprisingly loading capacity of company A C8 was only one hundredth to compare with SunShell C8 under acetonitrile/mm phosphate buffer ph.=(:) mobile phase. Company D C8 always showed poor peak of amitriptyline.

6 Relative retention/% Relative plate of butylbenzene/% Evaluation of Stability Accelerated acidic test 8 SunShell C8 Company D C8 Company B C8 Company A C8 Compnay C C8 % TFA 8 ºC 8 Durable test condition Column size: x. mm Time/h CH CN/.% TFA, ph=/9 Flow rate:. ml/min Temperature: 8 ºC Measurement condition Column size: x. mm CH CN/H =/ Flow rate:. ml/min Temperature: ºC Sample: = Uracil (t ) = Butylbenzene Stability under acidic ph condition was evaluated at 8 ºC using acetonitrile/% trifluoroacetic acid solution (:9) as mobile phase. % aqueous mobile phase expels from the pore of packing materials by capillarity and packing materials doesn t deteriorate. % acetonitrile in a mobile phase allows an accurate evaluation. -) Sunshell C8 has kept 9% retention for hours under such a severe condition. SunShell C8 is to times more stable than the other core shell C8. ) N. Nagae, T. Enami and S. Doshi, LC/GC North America ctober. ) T. Enami and N. Nagae, American Laboratory ctober. ) T. Enami and N. Nagae, BUNSEKI KAGAKU, () 9. Comparison column. Kinetex C8,. μm. Accucore C8,. μm. PoroShell C8 EC,. μm. Ascentis Express C8,. μm. SunShell C8,. μm 8 SunShell C8 Company D C8 Company A C8 Company B C8 Company C C8 ph ºC,,,,,, Durable test condition Column ze: x. mm Elution volume/ml CH H/mM Sodium borate/mm NaH=//9 (ph) Flow rate:. ml/min Temperature: ºC Measurement condition Column ze: x. mm CH CN/H =/ Flow rate:. ml/min Temperature: ºC Sample: = Butylbenzene Alkaline test Stability under basic ph condition was evaluated at ºC using methanol/sodium borate buffer ph (:) as mobile phase. Sodium borate is used as a alkaline standard solution for ph meter, so that its buffer capacity is high. Elevated temperature of ºC makes column life be one third. The other company shows stability test at ambient (room temperature). If room temperature is ºC, column life at room temperature ( ºC) is sixteen times longer than that at ºC. SunShell C8 is enough stable even if it is used under ph condition. Regarding stability under basic ph condition, there is little C8 column like SunShell C8 except for hybrid type C8. It is considered that our end-capping technique leads high stability. SunShell C8 can be used at the ph range from. to.

7 Efficiency of SunShell C8 UHPLC Column: SunShell C8, x. mm Plate()=, Column: SunShell C8, x. mm Plate()=, Column: SunShell C8,. mm x. mm CH CN/H =/ Flow rate:. ml/min Pressure: MPa Temperature: ºC Sample: = Uracil = Toluene = Acenaphthene = Butylbenzene Column: SunShell C8,. mm x. mm CH CN/H =/ Flow rate:. ml/min Pressure:. MPa Temperature: ºC 8 Column: SunShell C8, x. mm Efficiency=, plate/m 8 Plate()=8, Column: SunShell C8, x. mm Plate()=, HPLC 8% efficiency to compare with UHPLC Column: SunShell C8,. mm x. mm SunShell C8,. mm x. mm CH CN/H =/ Flow rate:. ml/min Pressure:.MPa(UHPLC),. MPa(HPLC) for mm 9.MPa(HPLC) for mm Temperature: ºC Sample: = Uracil = Toluene = Acenaphthene = Butylbenzene Column: SunShell C8, x. mm Plate()=, 8 8 LaChrom ELITE The same efficiency as mm, x. mm Saving % for analytical time and consumption of solvent

8 Theoretical Plate Examples of transfer from a conventional mm column to SunShell column Isocratic separation HPLC 8 8 N()=,8 SunShell C8,. mm x. mm Brand F C8, mm x. mm / of analysis time N()=9, N()=,8 Column: Brand F C8, μm x. mm SunShell C8,. μm x. mm CH CN/mM Phosphoric acid = / Flow rate:. ml/min,.8 ml/min at the lowest chromatogram Temperature: ºC Pressure: 9. MPa for Brand F C8 mm. MPa for SunShell C8. mm Detection: UV@ nm Sample: = Benzydamine = Ketoprofen = Naproxen = Indomethacin = Ibuprofen UHPLC. ml/min N()=, HPLC: Hitachi LaChrom ELITE ( 内径.mm の配管仕様 ) UHPLC: Jasco X-LC.8 ml/min N()=, Analysis time decreases to / to compare with a mm column sized x. mm. Comparison between normal and semi-micro HPLC Flow cell Response Sampling Tubing ID Normal. sec. sec. mm Semi-micro. sec. sec. mm Semi-micro. sec. sec. mm,,99,,,9,,,,, 9,,8 Uracil Toluene Acenaphthene Semi-micro. sec Semi-micro. sec Normal. sec Butylbenzene Peak width/sec Relationship between Peak width and theoretical plate 8 9 Comparison of chromatograms Column: SunShell C8, μm x. mm CH CN/H= / Flow rate:. ml/min Temperature: ºC Pressure:. MPa Detection: UV@ nm Sample: = Uracil = Toluene = Acenaphthene = Butylbenzene HPLC: Hitachi LaChrom ELITE Semi-micro HPLC derives near % performance of a core shell column. Even if normal HPLC is used, it derives 8% performance except for a narrow peak whose width is less than second

9 SunShell C8-WP, RP-AQUA, C8, Phenyl, PFP (Pentafluoropheny) Characteristics of SunShell Particle size Core shell silica Pore diameter Specific surface area Carbon content Bonded phase USP L# End-capping Maximum operating pressure Available ph range SunShell C8. mm 9nm m /g % C8 L Sunniest endcapping MPa. - SunShell C8-WP. mm nm 9 m /g % C8 L Sunniest endcapping MPa. - SunShell RP-AQUA. mm nm 9 m /g % C8 L Sunniest endcapping MPa - 8 a) SunShell C8. mm 9nm m /g.% C8 L Sunniest endcapping MPa. - 9 SunShell Phenyl. mm 9nm m /g % Phenylhexyl L Sunniest endcapping MPa. - 9 SunShell PFP. mm 9nm m /g.% Pentafluorophenyl L TMS endcapping MPa - 8 Comparison of standard samples a) Under % aqueous condition,, SunShell C8 SunShell PFP, SunShell Phenyl SunShell C8 SunShell RP-AQUA SunShell C8-WP Column: SunShell C8, C8-WP, RP-AQUA, C8, Phenyl, PFP,. μm x. mm CH H/H =/ Flow rate:. ml/min Temperature: ºC Sample: = Uracil = Caffeine = Phenol = Butylbenzene = o-terphenyl = Amylbenzene = Triphenylene Hydrogen bonding (Caffeine/Phenol) Hydrophobicity (Amylbenzene/Butylbenzene) Steric selectivity (Triphenylene/o-Terphenyl) PFP...8 Phenyl..8. C8...8 RP-AQUA... C8-WP SunShell C8.9.. Separation of peptides Separation of amitriptyline using C8 Separation of basic compounds SunShell C8-WP SunShell C8,, SunShell C8 SunShell PFP Column: SunShell C8-WP,. μm ( nm) x. mm, A).% TFA in Acetonitrile/water(:9) B). % TFA in Acetonitrile Gradient program: %B % % in min Flow rate:. ml/min, Temperature: ºC, Detection: UV@ nm, Sample: Tryptic digest of BSA Column: SunShell C8,. μm x. mm CH CN/mM phosphate buffer H.=/ Flow rate:. ml/min Temperature: ºC Detection: UV@nm Sample: = Uracil, = Propranolol, = Nortriptyline, = Amitriptyline Column: SunShell C8,. μm x. mm SunShell PFP,. μm x. mm CH CN/mM phosphate buffer ph.=8/ Flow rate:.8 ml/min Temperature: ºC Sample: = Uracil, = Propranolol, = Nortriptyline, =Amitriptyline 8

10 9 Separation of xanthines Separation of cresol isomers Separation of nucleotides SunShell C8 SunShell PFP Water Water SunShell C8, SunShell RP-AQUA N()=,9 N()=, SunShell PFP SunShell PFP mm phosphate buffer ph mm phosphate buffer ph SunShell PFP Column: SunShell C8, PFP,. mm x. mm CH H/water or buffer=/ Flow rate:. ml/min Temperature: ºC Detection: Sample: = Theobromine = Theophyline = Caffeine = Phenol Column: SunShell C8, PFP,. mm x. mm CH H/H =/ Flow rate:. ml/min Temperature: ºC Detection: UV@nm Sample: = p-cresol = m-cresol = o-cresol Column: SunShell RP-AQUA,. mm x. mm mm Phosphate buffer ph. Flow rate:. ml/min Temperature: ºC Detection: UV@nm Sample: = -GDP = -ATP = -ADP = -AMP Separation of nucleic acid bases High-throughput separation Sunniest RP-AQUA SunShell C8 SunShell RP-AQUA Column: Sunniest RP-AQUA, mm x. mm SunShell RP-AQUA,. mm x. mm mm Phosphate buffer ph. Flow rate:. ml/min for Sunniest. ml/min for SunShell Temperature: ºC Sample: = Cytosine, = Uracil, = Thymidine, = Uridine, = Thymine 8 Column: SunShell C8, x. mm. A) Water, B) Acetonitrile; Gradient (Acetonitrile %),. min - %,. min - %,.8 min - %,.8 min - %, cycle;.8min, (High-pressure gradient). Flow rate:. ml/min. Temperature: ºC. Injection Volume: µl. Wavelength: - nm, CH-9, - nm (Max Abs.). Sample: Mixture of ultraviolet absorbers, =,,, -Tetrahydroxybenzophenone, = Ethyl p-aminobenzoate, =, -Dihydroxybenzophenone, =, -Dihydroxy--methoxybenzophenone, =, -Dihydroxy-, -dimethoxybenzophenone, = -Hydroxy--methoxybenzophenone, = -( -Hydroxy- -metylphenyl) benzotriazole, 8 = -tert-butylphenyl salicylate. Courtesy of Jasco. A peak width is just one second!!

11 Desorption Dv (log d) (cc/g) Relative retention (%) SunShell HFC8-, HFC8- Characteristics of SunShell HFC8 Particl e size Core shell silica C8 (USP L# ) Pore diameter Specific surface area Carbon content Ligand density For separation of peptides and proteins End-capping Maximum operating pressure Available ph range SunShell C8-WP. mm nm 9 m /g %. μmol/m Sunniest endcapping MPa or 8, psi. - SunShell HFC8-. mm nm 9 m /g.%. μmol/m Sunniest endcapping MPa or 8, psi. 9 SunShell HFC8-. mm nm m /g.%. μmol/m Sunniest endcapping MPa a or 8, psi a. - 9 What is HFC8? Hexa-Functional C8 has six functional groups. This HFC8 is much more stable under acidic condition. a: MPa, psi for. mm i.d. column Sunniest Bonding Technology Hexamethydichlorotrisiloxane + Trimethylchlorosilane (TMS) (X: Cl, CH, C H ) Schematic diagram of reagent H Schematic diagram of the state of bonding on silica surface.9.8 Core shell nm. Core shell nm Pore Diameter (nm) Pore distribution of core shell particle 8 9% line.% formic acid, ph. ºC 8 Time (h) Stability under LC/MS mobile phase condition Durable test condition Column : SunShell HFC8-.mm, x. mm CH CN/.% formic acid, ph.=/ Flow rate:. ml/min Temperature: ºC Measurement condition CH CN/H =/ Flow rate:. ml/min Temperature: ºC Sample: = Uracil = Butylbenzene Separation of peptides SunShell HFC8- SunShell C8-WP peaks Column: SunShell HFC8-,. μm ( nm) x. mm, SunShell C8-WP,. μm ( nm) x. mm A).% TFA in Acetonitrile/water(:9) B). % TFA in Acetonitrile Gradient program: Flow rate:. ml/min Temperature: ºC Detection: UV@ nm Sample: Tryptic digest of cytochrome C

12 SunShell -EP For Supercritical fluid Chromatography. μm core shell column shows only one third of back pressure to compare with. μm fully porous column although both show almost same efficiency. By such low back pressure, a difference of density of supercritical fluid between an inlet and an outlet of the column is reduced. Consequently,.. μm core shell column performs a superior separation for SFC. Characteristics of SunShell -EP Particle size Core shell silica Pore diameter Specific surface area Carbon content Bonded phase Endcapping Maximum operating pressure Available ph range SunShell -EP. mm 9 nm m /g.% -Ethylpyridine no MPa or 8, psi. Comparison between SunShell -EP and. μm fully porous -EP Figure Figure : Chromatogram of the separation for he - component mix using the Sun Shell -EP x. mm column. A methanol gradient of < minutes was used on the Agilent Infinity SFC system. SFC conditions: flow rate:.ml/min; outlet pressure bar; column temperature ⁰C. Gradient program:.-.% in. min, then.-% in. min and held at % for. min. Figure : Chromatogram of the separation for the - component mix using Acquity UPC Viridis -EP x. mm column. of the components were resolved. A methanol gradient of < minutes was used on the Agilent Infinity SFC system. SFC conditions: flow rate. ml/min; outlet pressure bar; and column temperature ⁰C. Gradient program:.-.% in. min,.% for. min, then.-% in. min. Figure Courtesy of Pfizer Inc.

13 SunShell HILIC-Amide Characteristics of SunShell HILIC-Amide Particle size Core shell silica Amide (USP L# 8) Pore diameter Specific surface area For Hydrophilic Interaction Chromatography Carbon content Bonded phase End-capping Maximum operating pressure Available ph range SunShell HILIC-Amide. mm 9 nm m /g % Amide no MPa or 8, psi - 8 Stationary phase of HILIC-Amide R: Hydrophilic group Stationary phase of SunShell HILIC-Amide consists of AMIDE and HYDRPHILIC GRUP, so that this stationary phase is more polar than an individual group. High speed separation is leaded by core shell structure that derives high efficiency and fast equilibration. Separation of Nucleic acid bases: Comparison of the other core shell hilic columns SunShell HILIC-Amide S Company Core shell Polyol Column: SunShell HILIC-Amide,. μm x. mm, Coreshell polyol,. μm x. mm, Core shell lica,. μm x. mm Acetonitrile/ mm ammonium acetate(ph.) = 8/ Flow rate:. ml/min Temperature: o C Detection: UV@ nm Sample: = Thymine, = Uracil, = Uridine, = Cytosine, = Cytidine A Company Core shell lica Regarding retention of cytidine, SunShell HILIC-Amide showed % higher retention factor than S core shell polyol. Separation of Cyanuric acid and Melamine Separation of water- soluble vitamins..... Column: SunShell HILIC-Amide,. mm x. mm Acetonitrile/ mm phosphate Buffer (ph.9) =/ Flow rate:. ml/min Temperature: o C Detection: UV@ nm, Sample: = Cyanuric acid, = Melamine Column: SunShell HILIC-Amide,. mm x. mm Acetonitrile/ mm phosphate buffer (ph.) =8/ Flow rate:. ml/min Temperature: o C Detection: UV@ nm, Sample: = Nicotinic acid, = Ascorbic acid, = Pyridoxine

14 SunShell RP Guard Filter <Cartridge Type, Bonded with C8 and End-Capped with TMS> Available as a guard column for reversed phase Tubing.mmID, mm length Holder Before Cartridge filter bonded with C8 After The filter is made of porous glass sized mm i.d. and mm thickness. Pore diameter is mm. Low dead volume structure Back pressure on glass filter is ca.. MPa at. ml/min of flow rate. Upper pressure limit is more than MPa Available for. mm i.d to. mm i.d. column Evaluation of SunShell RP Guard Filter SunShell C8,. mm x. mm SunShell C8,. mm x. mm Without Guard Filter With Guard Filter t R ()=. min N() = 9,9 t R () =. min N() = 8,8 % decrease of plate CH CN/H =/ for. mm i.d. CH CN/H =/ for. mm i.d. Flow rate:. ml/min for. mm i.d..8 ml/min for. mm i.d. Temperature: ºC Detection: UV@nm Sample: = Uracil = Toluene = Acenaphthene = Butylbenzene Without Guard Filter With Guard Filter t R ()=. min N() = 9, t R () =. min N() = 8,9 Little change of plate Price of SunShell RP Guard Filter Name quantity Part number Photo SunShell RP Guard Filter For exchange pieces CBGAAC SunShell RP Guard Filter Holder piece CBGAAH

15 Distributor Biotech AB dichrom Labquip Bujino Chemicals Watrex Praha, s.r.o. Beijing Tai-Ke-Mei Hi-Tech Co., Ltd. brnuta faza j.d.o.o. Kinesis LabService Analytica S.r.l. Moricon BGB Analytik AG BGB Analytik France S.A.S. Imptech Scientific Yair Technologies Ltd IT Technologies Pte Ltd Nacalai USA, Inc. licycle, Inc. Innovations United I-Tek Instruments Chromatopak Analytical Prochrome India

16 rdering information of SunShell. μm SunShell C8 SunShell C8 SunShell PFP SunShell C8-WP SunShell RP-AQUA SunShell Phenyl SunShell HILIC-Amide SunShell -EP SunShell HFC8- SunShell HFC8- Inner diameter (mm).... Length (mm) Catalog number Catalog number Catalog number Catalog number CB9 CB CB CB CB9 CB CB CB9 CB CB CB CB9 CB CB CB CB9 CB CB CC9 CC CC CC9 CC CC CC9 CC CC CC9 CC CC CC9 CC CC CF9 CF CF CF9 CF CF CF9 CF CF CF9 CF CF CF9 CF CF CW9 CW CW CW9 CW CW CW9 CW CW CW9 CW CW CW9 CW CW CR9 CR CR CR CR9 CR CR CR9 CR CR CR CR9 CR CR CR CR9 CR CR CP9 CP CP CP9 CP CP CP9 CP CP CP9 CP CP CP9 CP CP CH9 CH CH CH9 CH CH CH9 CH CH CH9 CH CH CH9 CH CH CE9 CE CE CE9 CE CE CE9 CE CE CE9 CE CE CE9 CE CE CG9 CG CG CG9 CG CG CG9 CG CG C9 C C C9 C C C9 C C USP category L L L L L L L8 L L Manufacturer ChromaNik Technologies Inc. -- Namiyoke, Minato-ku, saka, - Japan TEL: FAX: info@chromanik.co.jp URL: