HPLC. Sorbtech Purity HPLC Columns. Macherey-Nagel HPLC Columns NUCLEODUR NUCLEOSHELL NUCLEOSIL. Mitsubishi MCI Gel HPLC Columns

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1 Sorbtech offers all types of metal jacketed columns made in-house with the highest quality materials available as well as columns manufactured by Macherey-Nagel, MCI Gel, and Jordi Labs. Sorbtech Purity Columns Macherey-Nagel Columns NUCLEODUR NUCLEOSHELL NUCLEOSIL Mitsubishi MCI Gel Columns Jordi Labs Columns Column Packing System 124

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3 Columns Sorbtech Purity Columns Sorbent Technologies introduces Purity the next generation of analytical and prep columns for small and large molecules (proteins & peptides) to maximize purification and purity of compounds. Purity is packed with Sorbtech s Premium Pure Silica Gels and uses a unique packing method to guarantee the best performance: resolution, reproducibility, and lifetime. Custom bonding and packing Purity is available in analytical, semi-prep, prep and custom sizes. Bonded Phases: C4, C8, C18, C18AQ, C18DE, Amino, Phenyl, Cyano, Diol, & Custom Particle Sizes: 2, 3, 5, & 10 μm Pore Sizes: 100, 150, 200, 300 & 500 Å Analytical: 2.0 to 4.6 mm ID, 50 to 250 mm L Prep: 10 to 100 mm ID, 50 to 250 mm L Let Purity optimize your method development and scale up from analytical to prep to process. Selectivity, Speed, Optimization, Loadability, Lifetime, Resolution, Reproducibility, Purity Custom Packing. We offer custom packing of Purity columns with Sorbtech adsorbents from analytical to preparative. Additionally, we can pack other manufacturer s resins from analytical to preparative. C18 AQ, 300 Å, 10 μm Mobile Phase: 60/40 Acetonitrile/Water Flow Rate: 1.0 ml/min Pressure: 375 psig Column Dimensions: 250 x 4.6 mm Detection: 254 nm Sample Size: 10 μl x250 Premium Pure C8 Silica Gel 5 μm 100 Å 4.6 x 250 mm x250 Premium Pure C18 Silica Gel 5 μm 100 Å 4.6 x 250 mm x250 Premium Pure C18 AQ Silica Gel 5 μm 100 Å 4.6 x 250 mm x250 Premium Pure C4 Silica Gel 5 μm 300 Å 4.6 x 250 mm x250 Premium Pure C8 Silica Gel 5 μm 300 Å 4.6 x 250 mm x250 Premium Pure C18 Silica Gel 5 μm 300 Å 4.6 x 250 mm Available in a wide selection of phases and sizes: analytical and preparative. For more selections and sizes, visit our website or contact your personal Product Specialist. 126

4 Macherey-Nagel Columns Macherey-Nagel TM is a world leader in chromatography products. Macherey-Nagel TM, known for industry standard NUCLEOSIL, introduces their new ultra-high purity NUCLEODUR products for. Nulceodur offers increased separation, reproducibility, reliability, and scalability. Now available in 1.8 μm for ultra-fast. 1.8 µm Particle Size for Ultra-Fast Macherey-Nagel NUCLEODUR NUCLEODUR is the new generation of totally spherical, high purity silica gels. These gels offer the best in separation performance and are now available in 1.8 µm for ultra-fast. Gravity, Isis, Pyramid, and Sphinx have unique bonding characteristics providing solutions for most separation applications. Ultra-High Purity High pressure stability Maximum Bed & ph Stability Maximize Efficiency High Separation Efficiency High Mechanical and chemical stability Increased load capacity Ideal particle shape and surface symmetry Reproducibility from lot to lot and column to column NUCLEODUR is now available in 1.8 µm for Ultra-Fast to allow extraordinary improvement in plate numbers, column efficiencies, and resolution compared to 3 µm. Decrease of analysis time (ultra fast ) Shorter columns with high separation efficiency Significant improvement of resolution Increased detection sensitivity All NUCLEODUR premium phases are available in 1.8 μm: C18 Gravity, C8 Gravity, C18 Isis, C18 Pyramid, Sphinx RP NUCLEODUR 1.8 μm particles are fractionated to limit the increase in back pressure. of 1.8 µm NUCLEODUR silica particles Increase of separation efficiency by higher number of theoretical plates (N): 50 x 4.6 mm NUCLEODUR C18 Gravity 3 µm: N 100,000 plates/m (h value 10) 1.8 µm: N 166,667 plates/m (h value 6) Increase of the plate number by app. 67% offers the possibility of using shorter columns with equal plate numbers resulting in a decrease of analysis time. Column back pressure Because of the high sphericity of the NUCLEODUR particles and the very narrow particle size distribution we are able to keep the back pressure on a moderate level. Nevertheless the use of columns packed with sub 2 µm particles generally makes special demands on the equipment. Pumps should be designer for pressures of bar and the entire system should feature the lowest possible dead volume. Higher flow rates and shorter run times Optimal flow rate for 1.8 µm particles is higher than for 3 µm and 5 μm particles (the flow rate should be at the van-deemter minimum). See van-deemter plot. Significant improvement in resolution Use of 1.8 μm instead of 3 µm particles leads to an increase of resolution by a factor 1.29 (29%) since the resolution is inversely proportional to the square root of the particle size: Van-Deemter plot column 50 x 4.6 mm, acetonitrile water (50:50, v/v), analyte toluene Resolution as a function of particle size Column: 50 x 4 mm NUCLEODUR C18 Gravity A) 3 µm, B) 1.8 µm Eluent: acetonitrile water (80:20, v/v) Flow rate: 2 ml/min Pressure: A) 80 bar, B) 160 bar Detection: UV, 254 nm 1. Naphthalene 2. Ethylbenzene α -1 k i ' R s = N ( ( 4 α k i '+1 ) ) R s = resolution α = selectivity (separation factor) k i ' = retention N = plate number with N 1/d P d P = particle size 127

5 NUCLEODUR C18 Gravity C8 Gravity NUCLEODUR Gravity columns are designed to handle high ph (up to 11), and can replace Waters TM Xterra TM, Phenomenex TM Luna TM, Zorbax TM Extend TM and other high end columns for pharmaceutical applications such as analgesics, anti-inflammatories, anti-depressants, herbacides, phytopharmaceuticals, immunosuppressants, etc. Preparative columns available by contacting your personal Product Specialist. Available as octadecyl (C18 USP L1) and octyl (C8 USP L7) modification Polymeric bonded phases have exceptionally long column lifetime and negligible phase leaching Pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm for C18, 1.8 and 5 μm for C8 7, 10, 12 and 16 μm particles for preparative purposes are available on request Ideal for method development Allows at ph extremes (ph 1 11) Suitable for LC/MS due to low bleeding characteristics Recommended for overall sophisticated analytical separations Compound classes separated so far: pharmaceuticals, e. g. analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosuppressants Surface Silanols at different ph values Influence of the ph value on selectivity Column: 125 x 4 mm NUCLEODUR C18 Gravity 5 µm Eluents: A) acetonitrile - 10 mm ammonium formate, ph 3.0 (50:50, v/v) B) acetonitrile - 10 mm ammonium bicarbonate ph 10.0 (50:50, v/v) Flow rate: 1.0 ml/min Temperature: 30 ºC Detection: UV, 230 nm Injection volume: 2 µl 1. Lidocaine 2. Benzamide 3. Ketoprofen The figure above shows the extent of protonation of surface silanols and of two exemplary analytes at acidic and alkaline ph. Separation of basic alkaloids Column: 125 x 4 mm NUCLEODUR C18 Gravity 5 µm Eluents: A) acetonitrile B) 20 mm (NH 4 ) 2 HPO 4, ph 2.5 / 10.0 Gradient: 10% A (1 min) -- 75% A in 10 min Flow rate: 1.0 ml/min Temperature: 25 ºC Detection: UV, 254 nm Injection volume: 2 µl 1. Lidocaine 2. Papaverine 3. Noscapine 4. Diphenydramine NUCLEODUR C18 Gravity 1.8 μm 110 Å 4.6 x 50 mm NUCLEODUR C18 Gravity 5 μm 110 Å 4.6 x 250 mm NUCLEODUR C8 Gravity 5 μm 110 Å 4.6 x 250 mm For more selections and sizes, visit our website or contact your personal Product Specialist. 128

6 NUCLEODUR C18 Isis NUCLEODUR C18 Isis offers high steric selectivity and unique surface modifications for steroids, aromatics and fat soluble vitamins. Isis replaces Inertsil TM ODS-P & YMC Pro C18RS. C18 phase with special polymeric, crosslinked surface modification USP L Pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm; 20% C Exceptional steric selectivity Outstanding surface deactivation Suitable for LC/MS due to low bleeding characteristics ph stability 1 10 Broad range of applications: steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins Tanaka plot of NUCLEODUR C18 Isis Steric selectivity of NUCLEODUR C18 Isis Column: 125 x 4 mm; 1 NUCLEODUR C18 Isis, 2 = mono merically coated C18 phase, 3 = polar endcapped phase Eluents: methonal - water (90:10, v/v) Flow rate: 1.0 ml/min, temperature: 35ºC Detection: UV, 254 nm Injection volume: 5 µl 1. o-terphenyl 2. m-terphenyl 3. p-terphenyl 4. Triphenylene NUCLEODUR C18 Isis 1.8 μm 110 Å 4.6 x 50 mm NUCLEODUR C18 Isis 5 μm 110 Å 4.6 x 250 mm NUCLEODUR C18 Pyramid NUCLEODUR C18 Pyramid is stable in 100% aqueous and designed for analgesics, penicillin antibiotics, nucleic acid bases, and more. Pyramid replaces Phenomenex TM Aqua TM, YMC AQ TM, & Waters TM Atlantis TM dc18. Separation of very polar compounds Column: NUCLEODUR C 18 Pyramid, 5 μm, 125 x 4 mm Eluent: 0.2% H 3 PO 4 Flow rate: 1.0 ml/min Temperature: 22 C Detection: UV, 202 nm Stable in 100 % aqueous mobile phase systems USP L1 Pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm; 14% C 7 and 10 μm particles for preparative purposes are available on request Interesting polar selectivity features Excellent base deactivation; suitable for LC/MS due to low bleeding characteristics ph stability 1 9 Classes of compounds separated so far: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agents, organic acids (injection volume 2 μl) 1. Formic acid 2. Acetic acid Tanaka plot of NUCLEODUR C18 Pyramid NUCLEODUR C18 Pyramid 1.8 μm 110 Å 4.6 x 50 mm NUCLEODUR C18 Pyramid 5 μm 110 Å 4.6 x 250 mm 129

7 NUCLEODUR - Sphinx RP Alternative RP selectivity NUCLEODUR Sphinx RP is characterized by exception- al selectivity features generated by a well-balanced ra- tio of covalently bonded octadecyl and phenyl groups. The combination of classical hydrophobic with π-π interactions (aromatic ring system) expands the scope of selectivity in comparison with conventional reversed phase packings. NUCLEODUR Sphinx RP is particularly suited for the separation of molecules containing aro- matic and multiple bonds. For the separation of polar compounds NUCLEODUR Sphinx RP can be especially recommended and can also outperform many custom- ary C18 phases. In addition, exhaustive endcapping steps minimize unwanted surface silanol activity and guarantee excellent peak shapes even for strong basic analytes. Stability of NUCLEODUR Sphinx RP at ph 10 Column: 50 x 4.6 mm NUCLEODUR Sphinx RP, 5 µm Eluent: methanol dil. NH 3, ph 10 (20:80, v/v) Flow rate: 1.0 ml/min, temperature 30 C Detection: UV, 275 nm Injection volume: 3 µl 1. Theophylline 2. Caffeine Different from standard phenyl phases, NUCLEODUR Sphinx RP is far more stable towards hydroly- sis and is also suggested for LC/MS applications. Due to the additional intermolecular interactions NUCLEODUR Sphinx RP is an interesting replenish- ment to the high density bonded phases NUCLEODUR C8/C18 Gravity and the polar endcapped NUCLEODUR C18 Pyramid. Distinct selectivity based on bifunctional surface coverage USP L1 and USP L11 Pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm; carbon content 15 % High density of covalently bonded silanes for tailing-free peaks Widens the scope for method development ph stability 1 10 Suitable for LC/MS due to low bleeding characteristics High reproducibility and consistent quality due to tight QC procedures Range of applications: quinolone antibiotics, sulfonamides, xanthines, substituted aromatics NUCLEODUR Sphinx RP Bifunctional 1.8 μm 110 Å 2 x 30 mm NUCLEODUR Sphinx RP Bifunctional 1.8 μm 110 Å 4.6 x 50 mm NUCLEODUR Sphinx RP Bifunctional 3 μm 110 Å 3 x 125 mm NUCLEODUR Sphinx RP Bifunctional 3 μm 110 Å 4.6 x 250 mm NUCLEODUR Sphinx RP Bifunctional 5 μm 110 Å 4.6 x 50 mm NUCLEODUR Sphinx RP Bifunctional 5 μm 110 Å 4 x 125 mm NUCLEODUR Sphinx RP Bifunctional 5 μm 110 Å 4.6 x 150 mm NUCLEODUR Sphinx RP Bifunctional 5 μm 110 Å 4.6 x 250 mm For more selections and sizes, visit our website or contact your personal Product Specialist. 130

8 NUCLEODUR - C18 ec / C8 ec NUCLEODUR C18 ec and C8 ec is ideal for daily routine reverse phase analysis and scale up to Prep. Exceptional silane bonding and endcapping allows for tailing-free elution of polar compounds, optimal chemical stability and ph stable from 1-9. These NUCLEODUR products replace Spherisorb, Hypersil, Waters Symmetry, Kromasil, & LiChrospher Medium density octadecyl (USP L1) and octyl phases (USP L7) Pore size 110 Å; particle sizes 3 μm and 5 μm; 7 μm, 10 μm, 12μm, 16 μm, 20 μm, 30 μm and 50 μm for preparative separations For daily routine analysis and up-scaling for preparative ph stability 1 9 High reproducibility from lot to lot For standard routine reversed phase applications ph stability of NUCLEODUR C18 ec Separation of theophylline and caffeine at ph 10 Column: 30 x 3 mm NUCLEODUR C18 ec Mobile phase: methanol aq. NH3 (20:80, v/v), ph 10 Flow rate: 0.5 ml/min Temperature: 25 C Detection: UV254 nm NUCLEODUR C18 ec 3 μm 110 Å 4.6 x 150 mm NUCLEODUR C18 ec 5 μm 110 Å 4.6 x 250 mm NUCLEODUR C8 ec 3 μm 110 Å 4.6 x 150 mm NUCLEODUR C8 ec 5 μm 110 Å 4.6 x 250 mm NUCLEODUR - HILIC High purity silica phase for Hydrophilic Interaction Liquid Chromatography Separation of Organic Acids on NUCLEODUR HILIC Ideal for reproducible and stable chromatography of highly polar analytes Suitable for analytical and preparative applications as well as LC-MS Very short column conditioning period Technical Characteristics: Ammonium sulphonic acid modified silica: pore size 110 Å, available particle sizes 1.8 µm, 3 µm and 5 µm; carbon content 7 %; ph stability Recommended Application: Hydrophilic compounds such as organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins. Separation of Organic Acids on NUCLEODUR HILIC Substances: fumaric acid; oxalic acid; citric acid Column: NUCLEODUR HILIC, 110 Å, 3 µm, 4 x 125 mm Phase: NUCLEODUR HILIC Cat. #: Conditions: Eluent: acetonitrile / 200 mm ammonium acetate, ph 6.8 (70:30, v/v) Flow rate: 1.0 ml/min Temperature: 25 C Injection volume: 0.5 µl Detection: UV, 220 nm NUCLEODUR Hilic 1.8 μm 110 Å 2 x 50 mm NUCLEODUR Hilic 1.8 μm 110 Å 2 x 100 mm NUCLEODUR Hilic 3 μm 110 Å 4.6 x 125 mm NUCLEODUR Hilic 3 μm 110 Å 4.6 x 250 mm NUCLEODUR Hilic 5 μm 110 Å 4.6 x 125 mm NUCLEODUR Hilic 5 μm 110 Å 4.6 x 250 mm 131

9 NUCLEODUR - CN / CN-RP NUCLEODUR is available in CN for normal phase and reverse phase CN-RP, widening the scope of selectivity. The cyano phase is stable against hydrolysis at low ph with a working range of ph 1-8. CN-RP is designed for polar organic compounds such as basic drugs, tricyclic anti-depressants, steroids, organic acids, and molecules with π electron systems. Pore size 110 Å; particle sizes 3 μm and 5 μm; 7% C USP L10 Multi-mode columns (RP and NP) Widens the scope in selectivity Different retention characteristics in comparison to C8 and C18 Stable against hydrolysis at low ph, working range ph 1 8 High reproducibility from lot to lot Classes of compounds separated so far: tricyclic antidepressants, steroids, organic acids Separation of cold medicine ingredients on two different NUCLEODUR phases Column: A) 250 x 4 mm NUCLEODUR C18ec B) 250 x 4 mm NUCLEODUR CN-RP Eluent: acetonitrile - 100mM sodium citrate ph 2.5 (15:85, v/v) Flow rate: 1.0 ml/min Temp.: 25 C Detection: UV270 nm, injection Volume: 10 μl 1. Maleic acid 2. Norephedrine 3. Ephedrine 4. Acetaminophen 5. Chlorpheniramine 6. Brompheniramine NUCLEODUR CN 5 μm 110 Å 4.6 x 125 mm NUCLEODUR CN 5 μm 110 Å 4.6 x 250 mm NUCLEODUR CN-RP 3 μm 110 Å 4.6 x 150 mm NUCLEODUR CN-RP 5 μm 110 Å 4.6 x 125 mm NUCLEODUR CN-RP 5 μm 110 Å 4.6 x 250 mm For more selections and sizes, visit our website or contact your personal Product Specialist. NUCLEODUR - NH 2 / NH 2 - RP High purity silica phase for Multi-mode columns (for RP, NP and IC) Stable against hydrolysis at low ph (working range ph 2 8), 100 % stable in water; suitable for LC/MS Widens scope of analytical into the polar range Technical characteristics: Aminopropyl-modified high purity silica; pore size 110 Å, particles sizes 3, 5 and 7 µm; 2.5 % C; not end-capped Reverse phase separation of sugars Column: NUCLEODUR NH 2 - RP 250 x 4 mm Eluent: acetonitrile-water (79:21, v/v) Flow rate: 2.0 ml/min Detection: RI 1. Fructose 2. Glucose 3. Saccharose 4. Maltose 5. Lactose Recommended application: Polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions Normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides Reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems Ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers NUCLEODUR NH 2 5 μm 110 Å 4.6 x 250 mm NUCLEODUR NH 2 -RP 3 μm 110 Å 4.6 x 150 mm NUCLEODUR NH 2- RP 5 μm 110 Å 4.6 x 250 mm For more selections and sizes, visit our website or contact your personal Product Specialist. 132

10 NUCLEOSHELL Core - shell silica for Demands on separations are constantly increasing with respect to separation efficiency and detection limits as well as concerning the time requirements for each analysis. Core-shell technology sets new standards for increasing efficiency in research and quality control. Solid core of silicon dioxide, homogeneous shell of porous silica Highest efficiency compared to traditional totally porous materials Pore size 90 Å; particle size 2.7 µm (core 1.7 µm), specific surface 130 m2/g RP 18 phases are also available with particle size 5 µm (core 3.8 µm), specific surface 90 m2/g Lower back pressure also allows use on conventional LC systems Pressure stability up to 600 bar Phase Modification % C Particle size [µm] ph range USP Structure RP 18 Octadecyl, multi-endcapping 97 % L1 RP 18plus Octadecyl (monomeric), multiendcapping L1 Phenyl- Hexyl Phenyl-Hexyl, multiendcapping 4.5 2, L11 PFP Pentafluorophenyl, multiendcapping ~ 3 2,7 1 9 L43 HILIC Ammonium sulfonic acid 1.3 2, Benefits of Core-Shell Technology Short diffusion paths Rapid mass transfer (C term of the Van Deemeter equation) High flow velocity without peak broadening for fast LC Narrow particle size distribution (d90/d10~1.1) Stable packing High heat transfer minimized influenced of frictional heat column efficiency of NUCLEOSHELL ~250000/m (HETP ~ 4µm) 133

11 NUCLEOSHELL Core - shell silica for Utilizing a proprietary process of synthesis, NUCLEOSHELL particles exhibit a distinct narrow particle size distribution (d90/d10 ~ 1.1). Columns packed with NUCLEOSHELL core shell particles feature exceptional separation efficiencies with theoretical plate numbers easily comparable to totally porous sub 2 micron particles. In direct comparison with the con- ventional sub 2 micron phases, NUCLEOSHELL columns only generate about 60 % of the back pressure and can be operated with the majority of conventional systems. In order to develop the maximum performance of NUCLEOSHELL columns, we recommend reducing extra column voids by using suitable capillaries (< 0.15 mm inner diameter) and specially adapted detector cells. Moreover detector settings should be optimized by increasing the measuring rate or by decrease of the time constant. Resolution R s as a function of particle size Column: 50 x 4 mm each NUCLEOSHELL RP 18, 2.7 µm NUCLEODUR C18 Gravity, 3 µm NUCLEODUR C18 Gravity, 1.8 µm Eluent: acetonitrile - water (60:40, v/v) Flow rate: 1 ml/min Temperature: 25 ºC Detection: UV, 254 nm 1. Naphthalene 2. Ethylbenzene Better resolution at lower back pressure and shorter retention time Van - Deemter plots The Van Deemter plots demonstrate how efficiency is affected by flow rate. In comparison with fully porous silicas, core-shell particles from various manufacturers maintain the efficiency optimum (max. plates/m) over a long range of increasing linear mobile phase velocity. Van - Deemter plots 134

12 NUCLEOSHELL Core - shell silica for Pressure Drop Pressure Drop In direct comparison with conventional sub 2 micron phases, NUCLEOSHELL columns only generate about 60% of the back pressure and can be operated with the majority of conventional systems. In order to develop the maximum performance of NUCLEOSHELL columns, we recommend reducing extra column voids by using suitable capillaries (<0.15 mm inner diameter) and specially adapted detector cells. Moreover detector settings should be optimized by increasing the measuring rate or by decrease of the time constant. of core-shell silica particles A criterion for the long-term stability of the column at ph extremes is the percentage decrease of initial retention and initial plates, respectively. The column can also be operated at elecated temperatures without loss in retention behavior, efficiency or peak symmetry. The Sability under acidic and basic conditions graph shows a column stability test of NUCLEOSHELL RP 18 at mobile phase levels ph 1 and ph 10 compared with three competing phases. Stability under acidic and basic conditions Column: 50 x 4.6 mm NUCLEOSHELL RP 18, 2.7µm 50 x 4.6 mm Kinetex 2.6 µm C18 Eluent: acetonitrile - 1% TFA in H2O, ph 1 (50:50, v/v) Flow rate: 1.3 ml/min; temperature 80 ºC Detection: UV, 254 nm Sample: anthracene Uniformly shaped NUCLEOSHELL particles combined with optimized bonding technology safeguards tightly packed columns for 100% reproducible results. Temperature stability Column: 50 x 4.6 mm NUCLEOSHELL RP 18, 2.7µm Eluent: A) 10 mmol/l ammonium formate - methanol (9:1, v/v) µl formic acid, ~ ph 4 B) 10 mmol/l ammonium formate - methanol (1:9, v/v) µl formic acid, ~ ph % B in 7 min. Flow rate: 0.5 ml/min Temperature: 100 ºC Detection: UV, 220 nm 1. Phenol 2. Naphthalene Column: 50 x 4.6 mm NUCLEOSHELL RP 18, 2.7µm 50 x 4.6 mm Ascentis Express C18, 2.7 µm 50 x 4.6 mm Poroshell 120 EC-C18 50 x 4.6 mm Kinetex 2.6 µm C18 Eluent: 20 mmol/l Na borate - 10 mmol/l NaOH - methanol, ph 10 (21:49:30, v/v)) Flow rate: 1.5 ml/min; temperature 40 ºC Detection: UV, 220 nm Sample: toluidine Efficiency test: Eluent: acetonitrile - water (60:40, v/v) Flow rate: 0.33 ml/min Temperature: 25 ºC Detection: UV, 254nm Sample: anthracene HETP[µm] Asymmetry Start (t = 0) End (t = 40 h)

13 NUCLEOSHELL Core - shell silica for Loading capacity Columns: 50 x 3 mm NUCLEOSHELL RP 18, 2.7 µm Eluent: acetonitrile - 25 mmol/l KH2PO4, ph 3 (70:30, v/v) Flow rate: 0.66 ml/min, temperature 30 ºC Detection: UV, 285 nm 1. Valerophenone Batch-to-batch reproducibility Column: 50 x 4 mm NUCLEOSHELL RP 18, 2.7 µm Eluent: methanol - 25 mmol/l KH2PO4 ph 7 (70:30, v/v) Flow rate: 1mL/min Temperature: 40 ºC Detection: UV, 254 nm 1. Uracil 2. Toluene 3. Ethylbenzene 4. Acenapthene 5. Amitriptyline 6. o-terphenyl 7. Triphenylene Peak capacity Columns: 100 x 4.6 mm each NUCLEOSHELL RP 18, 2.7 µm NUCLEODUR C18 Gravity, 1.8 µm NUCLEODUR C18 Gravity, 3 µm NUCLEODUR C18 Gravity, 5 µm A) acetonitrile, B) water, % A in 4 min. Flow rate: 1.5 ml/min; temperature 25 ºC Detection: UV, 230 nm 1. Acetophenone 3. Propiophenone 5.Benzophenone 2. Benzoin 4. Butyrophenone 6. Valerophenone Cephalosporin antibiotics Comparison of 5 µm core-shell and totally porous phases Columns: 100 x 4.6 mm each A) NUCLEOSHELL RP 18plus, 5 µm B) NUCLEODUR Gravity C18, 5 µm Eluent: Methanol - water + 0.1% formic acid (35:65, v/v) Flow rate: 1.3 ml/min Pressure: 182 bar, 219 bar Temperature: 25 ºC Detection: UV, 254 nm Injection: 4.0 µl Max. Pressure Resolution [bar] (4, 5) NUCLEOSHELL, 2.7 µm NUCLEODUR, 1.8 µm NUCLEODUR, 2.7 µm NUCLEODUR, 2.7 µm Ret. time Asymmetry Plates [min] (EP) (EP) A B A B A B 1. Cefotaxime Cefoxitin Cefamandole Cefalotine

14 NUCLEOSHELL RP 18 - Core-shell technology silica phase for NUCLEOSHELL RP 18 combines innovative silica technology and excellent surface deactivation, that outperforms conventional C18 silicas in terms of efficiency, resolution and speed. Due to the applied core-shell particle design the back pressure at elevated flow rates remains at a moderate level and permits the use of existing equipment in many cases. NUCLEOSHELL RP 18 with extended ph stability, low bleed characteristics in LC/MS applications and overall robustness is an ideal tool for method development and routine analysis in modern. Based on core-shell particle technologyfor fast and efficient Suitable for LC/MS and at ph extremes (ph 1-11) Superior base deactivation, ideal for method development 13 ß-lactam antibiotics in less than 3 min Parameters of the Tanaka diagram Capacity = k (pentylbenzene) Hydrophobicity = a(pentylbenzene, butylbenzene) Steric selectivity = a(triphenylene, o -terphenyl) Hydrogen bonding capacity (silanol capacity) =a(caffeine, phenol) Ion exchange capacity at 2 different ph values (2.7 and 7.6) = a(benzylamine, phenol) The separation of 13 ß-lactam antibiotics illustrates how time of analysis can be shortened to a fractional part by using core-shell particles without loss of resolution at moderate back pressure. Columns: 50 x 4 mm NUCLEOSHELL RP 18, 2.7 µm 150 x 4 mm NUCLEODUR C18 Gravity 5 µm Eluent: A) acetonitrile; B) 20 mmol/l KH2PO4 ph % A (0.5 min) -> 50% A in 1.5 min. (0.5 min 50% A) 10% A (3 min) -> 50% A in 9 min (3 min 50% A) Flow rate: 2 ml/min, 1 ml/min Pressure: 270 bar, 110 bar Temperature: 25 ºC Detection: UV, 220 nm 1. Amoxicillin 6. Cafemandole 11. Cloxacillin 2. Ampicillin 7. Cephalothin 12. Nafcillin 3. Cephalexin 8. Piperacillin 13. Dicloxacillin 4. Cefotaxime 9. Penicillin V 5. Cefoxitin 10. Oxacillin NUCLEOSHELL RP µm 90 Å 50 x 4 mm NUCLEOSHELL RP µm 90 Å 100 x 4 mm NUCLEOSHELL RP µm 90 Å 150 x 4 mm 137

15 NUCLEOSHELL RP 18plus - Core-shell technology silica phase for NUCLEOSHELL PR 18plus is a C18 modified core- shell silica. Due to a monomeric bonding chemistry this phase offers hydrophobic characteristics with distinct polar selectivity. A special derivatization process generates a medium density of bonded silanes (carbon content 5.7 % for 2.7 μm, 4.4 % for 5 μm) with reduced steric selectivity compared to NUCLEOSHELL RP 18. Due to its low bleeding characteristics NUCLEOSHELL RP 18plus is suitable for LC/MS applications. In addition NUCEOSHELL RP 18plus provides a good stability under highly aqueous conditions. Even by long term usage or storage of the phase hardly phase collapse and loss of retention are observed. The original performance can be regained after a short regeneration procedure. Monomeric octadecyl modification, multi-endcapped; pore size 90 Å; particle size 2.7 μm with carbon content 5.7 %, particle size 5 µm with 4.4 % C; ph stability 2 9; suitable for LC/MS. Based on core-shell particle technology for fast and efficient Hydrophobic C18 phase with distinct polar selectivity, ideal for method development Excellent performance under highly aqueous conditions Bleeding Characteristics Columns: 50 x 2 mm NUCLEOSHELL RP 18plus 2.7 µm Eluent: A) water + 0.1% formic acid, B) acetonitrile + 0.1% formic acid; 95% A -> 5% A in 4.5 min (0.5 min) -> 95% A in 0.5 min (4.5 min) Flow rate: 0.5 ml/min Temperature: 25 ºC Detection: MS Tanaka plot of NUCLEOSHELL RP 18plus The more polar characteristics of NUCLEOSHELL RP 18plus compared to NUCLEOSHELL RP 18 are demonstrated by the lower capacity (retention of pentylbenzene). Also a comparison of retention of the glycopeptide antibiotic vancomycin on several octadecyl modified core-shell phases underlines the polar selectivity of NUCLEOSHELL RP 18plus. The Extremely reduced silanol activity of the phase can be demonstrated by the low ion exchange capacity at ph 7.6 which indicates excellent endapping. The radar diagram also shows that the monomeric bonding chemistry results in a thinner carbon coating and less steric selectivity. NUCLEOSHELL RP 18plus combines superbly hydrophobic and polar selectivity - so it is a useful tool for method development in RP chromatography. Good ph stability and low bleeding characteristics make it ideal especially for LC/MS applications NUCLEOSHELL RP18plus 5 µm 90 Å 4 x 100 mm NUCLEOSHELL RP18plus 5 µm 90 Å 4.6 x 100 mm NUCLEOSHELL RP18plus 5 µm 90 Å 4.6 x 250 mm 138

16 NUCLEOSHELL Phenyl-Hexyl - Core-shell technology silica phase for Phenyl-Hexyl modified phases offer an excellent separation efficiency especially for aromatic and unsaturated compounds with electronwithdrawing groups. The combination of hydrophobic and π-π interactions results in an alternative and interesting selectivity profile compared the C18 or C8 modifications. NUCLEOSHELL Phenyl- Hexyl is based on an unique surface bonding chemistry - therefore it is suitable for LC/MS due to low bleeding characteristicsnand offers high temperature stability and ph stability from 1 to 10. The separation of sulfonamides proves the scalability from fully porous NUCLEODUR to NUCLEOSHELL Phenyl-Hexyl. Hereby the core-shell silica exhibits under same conditions identical selectivity, narrower peaks and slightly shorter retention. Thus, method transferability between NUCLEODUR and NUCLEOSHELL is guaranteed, either for speeding up your methods or scaling up for preparative requirements. The pyridine-phenol test shows that NUCLEOSHELL Phenyl-Hexyl provides a symmetrical peak for pyridine and higher resolution in comparison to other core-shell based Phenyl-Hexyl phases, which underlines the excellent base deactivation. Phenyl-Hexyl modification, multi-endcapped; pore size 90 Å, particle size 2.7 µm; carbon content 4.5%; ph stability 1-10; suitable for LC/MS Based on core-shell particle technology for fast and efficient Hydrophobic phase with alternative selectivity compared to classical C18 modifications Separation principle based on 2 retention mechanisms: Ï -Ï interactions and hydrophobic interactions Stability of NUCLEOSHELL Phenyl-Hexyl at ph 10 Columns: 50 x 4 mm NUCLEOSHELL Phenyl-Hexyl 2.7µm Eluent: acetonitrile - 50 mmol/l TEA ph 10 (60:40, v/v); ph of the mixture 10.4 Flow rate: 1 ml/min Temperature: 40 ºC Detection: UV, 254 nm Injection: 1µL 1. Phenol 2. Naphthalene 3. Anthracene NUCLEOSHELL Phenyl-Hexyl is a robust phase with an alternative RP selectivity for aromatic and unsaturated analytes compared to classical C18 / C8 phases it is an additional and useful tool for all chromatographers. Bleeding Characteristics of NUCLEOSHELL Phenyl-Hexyl Columns: 50 x 2 mm each NUCLEOSHELL Phenyl-Hexyl, 2.7 µm Kinetex Phenyl-Hexyl Eluent: A) acetonitrile, B) water; 5-95% A in 25 min Flow rate: 0.2 ml/min Temperature: 25ºC Detection: MS NUCLEOSHELL Phenyl-Hexyl 2.7 µm 90 Å 3 x 150 mm NUCLEOSHELL Phenyl-Hexyl 2.7 µm 90 Å 4 x 150 mm NUCLEOSHELL Phenyl-Hexyl 2.7 µm 90 Å 4.6 x 150 mm 139

17 NUCLEOSHELL PFP - Core-shell technology silica phase for Fluorinated stationary phases in have gained increasing interest over the last years. Most common representative of fluorinated silica phases is the pentafluorophenyl modification (PFP or F5). Especially the orthogonal selectivity compared to traditional alkyl phases widens the scope in analytical. Thus NUCLEOSHELL PFP offers an excellent selectivity especially for highly polar analytes, aromatic and unsaturated compounds, phenols or halogenated hydrocarbons. Halogen substitutes in molecules result often in an increase of their polarity accompanied by a decrease of typical retention characteristics in RP. While a typical C18 phase just provides hydrophobic interactions between stationary phase and analyte NUCLEOSHELL PFP offers four different retention mechanisms: polar interactions (H bonds), dipoledipole interactions, π-π interactions and hydrophobic interactions. Especially the pronounced ion exchange capacity and distinct steric selectivity are typical for the character of fluorinated phases. Based on core-shell particle technology for fast and efficient Hydrophobic phase with alternative selectivity in comparison to classical C18 modifications Separation principle based on 4 retention mechanisms: polar interactions (H bonds) dipole-dipole interactions π-π interactions hydrophobic interactions NUCLEOSHELL PFP combines the benefits of core-shell technology, high stability and orthogonal selectivity. So it is a useful complementary tool for highly efficient separations especially of isomers, halogenated, aromatic and/or polar compounds. Stability of PHP at ph1 Columns: 100 x 4,6 mm NUCLEOSHELL PFP, 2.7 µm 100 x 4.6 mm Kinetex 2.6 µm Eluent: acetonitrile - 0.5% TFA ph 1 (50:50, v/v) Flow rate: 1.3 ml/min Temperature: 60 ºC Detection: UV, 254 nm Sample: Ethylbenzene Methylacetophenones Columns: 100 x 4,6 mm NUCLEOSHELL PFP, 2.7 µm 100 x 4.6 mm NUCLEODUR PFP, 5 µm Eluent: methanol - water (35:65, v/v) Flow rate: 1.5mL/min, 3 ml/min, 1 ml/min, 1.5 ml/min Temperature: 35 ºC Detection: UV, 254 nm 1. o-methylacetophenone 2. p-methylacetophenone 3. m-methylacetophenone NUCLEOSHELL PFP 2.7 µm 90 Å 2 x 100 mm NUCLEOSHELL PFP 2.7 µm 90 Å 3 x 100 mm NUCLEOSHELL PFP 2.7 µm 90 Å 4 x 100 mm 140

18 NUCLEOSHELL HILIC - Core-shell technology silica phase for Hydrophilic interaction chromatography (HILIC) is a separation technique using polar stationary phases and organic-aqueous mobile phases. A minimum water content of at least 2% is indispensable to provide a permanent water layer between the adsorbent surface and the organic fraction of the mobile phase. The sample molecules become separated in a partition chromatography, in which polar analytes are more strongly retained than neutral, less hydrophilic compounds. Consequently, increasing the aqueous part in the mobile phase will diminish retention of the polar sample constituents. In this way HILIC behaves inverse to classical RP chromatography. The particular retention profile of HILIC enables the chromatography of very polar and often small molecules, which won't show any retention on C8 or C18 reversed phases. Ultra-fast separations at moderate back pressure NUCLEOSHELL HILIC is a core-shell technology based stationary phase with a covalently bonded 3-N,N-dimethylaminopropane sulfonic acid ligand (pat. pend.). The betaine character of the strong ion-exchanger results in full charge balancing and facilitates fast equili- bration times. Good separation of polar compounds like the physiologically important substances creatine and creatinine can be achieved on NUCLEOSHELL HILIC as well as on NUCLEODUR HILIC, 1.8 μm at similar retention, but much lower back pressure. Based on core-shell particle technology for fast and efficient Ideal for reproducible and stable chromatography of highly polar analytes Very short column equilibration times Separation of creatine and creatinine Columns: 50 x 4 mm NUCLEOSHELL HILIC, 2.7 µm 50 x 4 mm NUCLEODUR HILIC, 1.8 µm Eluent: acetonitrile - 10 mmol/l ammonium acetate ph 4.0 (90:10, v/v) Flow rate: 1.7 ml/min Pressure: 129 bar 180 bar Teperature: 25 ºC Detections: UV, 210 nm 1. Creatinine 2. Creatine Separation of catecholamine Columns:100 x 4 mm NUCLEOSHELL HILIC, 2.7 µm 100 x 4 mm NUCLEOSHELL HILIC, 2.7 µm 250 x 4 mm NUCLEODUR HILIC, 3 µm Eluent: acetonitrile mmol/l ammonium formate ph 3.2 (80:20, v/v) Flow rate: 4 ml/min, 1 ml/min, 1 ml/min Pressure: 395 bar, 95 bar, 116 bar Teperature: 25 ºC Detections: UV, 280 nm 1. DOPAC 2. Serotonin 3. Dopamine 4. Epinephrine 5. Norepinephrine 6. DOPA 7. DOPS NUCLEOSHELL HILIC 2.7 µm 90 Å 3 x 50 mm NUCLEOSHELL HILIC 2.7 µm 90 Å 4 x 50 mm NUCLEOSHELL HILIC 2.7 µm 90 Å 4 x 50 mm 141

19 NUCLEOSIL The standard in is a family of completely porous spherical silicas that offers optimized bonding phases for every application. An incredible selection of RP phases that include Nautilus for 100% aqueous applications, as well as, C18, C8, C4, C2, Amino, Phenyl, Cyano, Nitro, Diol, Dimethylamino, Sulphonic Acid, Quaternary Ammonium, SAX, SCX, Chiral, and more. Packing sizes from 3 μm to 10 μm and pore sizes from 100 Å High bed stability due to spherical particles High efficiency due to narrow particle size distribution High separation performance due to optimized binding techniques Reproducibility from lot to lot and column to column to 4000 Å High load capacity and recovery rates High mechanical and chemical stability Available in analytical, semi-prep, and prep columns Custom columns are also available Sorbent Technologies, Inc.

20 Analysis of nitroaromatic compounds (explosives) Determination of Polyamines in Waste Water Column: ChromCart, NUCLEOSIL C18, 120 Å, 3 μm, 4 x 125 mm Phase: NUCLEOSIL C18, 120 Å, 3 μm Catalog #: Substances: Diamino-4-nitrotoluene-26 diamino-4-nitrotoluene-24 hexogene hexahydro-135-trinitro-135-triazine trinitrobenzene-135 methyl-3-nitroaniline-2 methyl-5-nitroaniline-2 methyl-3- nitroaniline-4 dinitrobenzene-13 trinitrotoluene-246 amino-26- dinitrotoluene-4 am. Conditions: Eluents: A) Methanol B) Water Gradient: 35% A in 12 min. to 55% A, isocratic till 52 min., then 100 % A Temperature: 30 C Injection vol.: 40 μl Flow rate: 0.35 ml/min. Column: NUCLEOSIL C18, 100 Å, 5 μm, 4 x 250 mm Phase: NUCLEOSIL C18, 100 Å, 5 μm Catalog #: Substances: hydroxyputrescine diaminopropane putrescine cadaverine diaminooctane spermidine sym-homospermidine homospermidine Information: Derivatisation with dansylchlorid Conditions: Eluent: A: acetonitrile B: water Gradient: 60 to 15% B in 35 min. Temperature: 40 C Flow rate: 1.0 ml/min Detection: Fluorescence NUCLEOSIL C18 5 μm 100 Å 4 x 250 mm NUCLEOSIL C18 5 μm 120 Å 4.6 x 250 mm NUCLEOSIL C8 5 μm 120 Å 4.6 x 250 mm NUCLEOSIL C4 5 μm 300 Å 4.6 x 250 mm NUCLEOSIL NH 2 (Amino) 5 μm 100 Å 4.6 x 250 mm NUCLEOSIL CN-RP (Cyano) 5 μm 100 Å 4.6 x 250 mm 143

21 / Polymer MCI GEL - Highly Stable Polymeric Analytical Columns We offer MCI GEL columns with reversed phase, size exclusion, ion exchange and hydrophobic polymer based packings for proteins, oligosaccarides, peptides, amino acids and other applications. The use of polymeric based columns has become more widespread thanks to unique selectivity of the polymer matrix that has no specific adsorption common with silica based packings. These columns can be operated with a wide ph range both basic and acidic eluents due to the chemical stability of the inert polymeric materials. Columns are available in analytical, semi-prep and prep sizes. MCI GEL reversed phase columns are based on a polystyrenic and polymethacrylate porous polymers. These columns are normally applied to the separation of aromatic and aliphatic based compounds in the isocratic and gradient elution modes. The applications include pharmaceuticals, steroids, small peptides, amphoteric molecules such as sulfonamides and cephalosporin antibiotics, plus basic drugs, simple amines, antihistamines and carbamate pesticides. Separation of proteins mixture Column: Eluent: Flow rate: Col. Temp.: Detection: Sample: Conditions MCI GEL CMG20/C mm I.D. x 250 mm A) 0.05% TFA/CH 3 CN=80/20 B) 0.05% TFA/CH 3 CN=30/70 A -> B 45 min linear gradient 0.5 ml/min 25 C 280 nm 1. Ribonuclease A 2. Cytochrome C 3. Transderrin 4. α-chymotrypshinogen A 5. β-lactoglobulin Excellent separation performance Chemically stable Wide ph range (very strong acids & bases) Long Lifetime, easily cleaned No irreversible binding associated with silica based columns Accurate results Reversed phase resins CHP20/C04 and CHP20/C10 are recommended for analysis of more hydrophobic substances. CMG20/C04 and CMG20/C10 are recommended for hydrophilic samples. Cat. # Description Particle Size ID x L ph MCI GEL CHP20/C04 Polystyrene RP 4 μm 4.6 x 150 mm MCI GEL CHP20/C10 Polystyrene RP 10 μm 4.6 x 250 mm MCI GEL CHP07/C04 Modified Polystyrene RP 4 μm 4.6 x 150 mm MCI GEL CHP07/C10 Modified Polystyrene RP 10 μm 4.6 x 250 mm MCI GEL CMG20/C04 Polymethacrylate RP 4 μm 4.6 x 150 mm MCI GEL CMG20/C10 Polymethacrylate RP 10 μm 4.6 x 250 mm 2-12 CK & CA are cation & anion exchange columns for food applications for sugars and oligosaccarides. Cat. # Description Particle Size ID x L Counter Ion MCI GEL CK08S Cation 11 μm 8 x 500 mm Na MCI GEL CK08E Cation 9 μm 8 x 300 mm Na MCI GEL CA08F Anion 7 μm 4.6 x 250 mm Cl- SCK & SA are surface functionalized resins for non-suppressed ion chromatography applications. Cat. # Description Particle Size ID x L Counter Ion MCI GEL SCK01 Cation 9 μm 4.6 x 150 mm H MCI GEL SCA04 Anion 5 μm 4.6 x 150 mm Cl- CQP are designed for bioseparation for size exclusion chromatography. Cat. # Description Particle Size ID x L Pore Size MCI GEL CQP10 10 μm 7.5 x 600 mm 200 Å For more selections and sizes, visit our website or contact your personal Product Specialist. 144

22 MCI GEL Separation of Phthalic Acid Esters Amines Column: Eluent: Flow rate: Col. Temp.: Detection: Sample: Conditions MCI GEL CHP20/C mm I.D. x 150 mm H 2 O/CH 3 CN=50/ ml/min 60 C 254 nm 1. Dimethyl phthalate 2. Diethyl phthalate 3. Dipropyl phthalate Conditions Column: MCI GEL SCK01 6 mm I.D. x 50 mm Eluent: 5mM HNO 3 Flow rate: 1.0 ml/min Col. Temp.: 40 C Detection: Conductivity 254 nm Separation of Proteins Mixture Standard Anions Conditions Column: MCI GEL ProtEx-DEAE 4.6 mm I.D. x 50 mm Eluent: A) 20mM Tris-HCl ph 8.0 B) A + 0.5M NaCl A -> B 30 min linear gradient Flow rate: 0.5 ml/min Col. Temp.: ambient Detection: 280 nm Sample: 1. Myoglobin 25 μg 2. Conalbumin 25 μg 3. Trypsin inhibitor 50 μg Column: Eluent: Flow rate: Col. Temp.: Detection: Sample: Conditions MCI GEL SCA mm I.D. x 50 mm 3mM VA 2.8mM MDEA ph ml/min 40 C Conductivity 1. F - 2. H 2 PO 4-3. Cl - 4. NO 2-5. Br - 6. NO3-7. SO 4 2- ProtEx Series MCI GEL ProtEx series packed columns are for ion exchange chromatography which separate sample proteins mainly via ionic interaction between packing material and sample molecules. The packing materials for ProtEx series columns are based on 5 μm, mono disperse, porous type, methacrylate polymer, and are specifically designed for separation of proteins. On a conventional protein separation column, non-specific adsorption of sample proteins sometimes occurs resulting in loss of valuable sample. But on the ProtEx columns, non-specific adsorption is eliminated because the surface of the packing material is surrounded by a hydrophilic layer which is chemically bonded to the base material, and therefore, ion exchange of functional groups is increased. ProtEx series are for ion exchange for sample proteins. Cat. # Description Particle Size ID x L ph MCI GEL ProtEx-DEAE 5 μm 7.5 x 100 mm MCI GEL ProtEx-SP 5 μm 7.5 x 100 mm

23 Mitsubishi MCI GEL - Chiral Separation Columns Mitsubishi MCI GEL CRS10W and its companion product MCI GEL CRS15W (an optical isomer of CRS10W) are based on a 3 μm with 100 Å mean pore diameter of silica gel coated with N, N-Dioctyl -L(or D)-alanine which is a novel optical activated ligand. The chiral resolution mechanism is a combination of ligand exchange and hydrophobic interaction. A copper sulfate aqueous solution is used as an eluent. Elution samples are directly detected at a wave length of 254 nm because the complex compound, composed of the sample molecule and copper in the eulent, are the objects of detection. With the CRS10W, D-isomers generally elute in front of L-isomers while L-isomers elute ahead of D-isomers on the CRS15W. The hydrophobic interaction mechanism allows hydrophilic samples to elute faster than hydrophobic molecules. Long alkyl chain or aromatic compounds will elute late or require an organic solvent (CH 3 CN or CH 3 OH, max. of 15 v/v%) to prevent absorption onto the stationary phase. Advantages: The CRS series columns separate over 20 D,L-α-Amino acids by only single column. The columns separate not only α-amino acids but also α-hydroxy carboxylic acids and derivative amino acids such as Acetylated amino acids. The columns provide excellent resolution operated at room temperature. The columns show high durability. Separation mechanisms and characteristics Application of CRS10W DL-Isoleucine Separation of amino acids mixture Separation of DL-tryptophan Durability test CRS10W (DLAA) 3 μm 100 Å 4.6 x 50 mm CRS15W (LDAA) 3 μm 100 Å 4.6 x 50 mm 146

24 / GPC Jordi Gel - Permeation Chromatography of Oligomers and Polymers Jordi FLP manufactures a complete line of polymer-based high-pressure liquid chromatography columns specializing in size exclusion chromatography. All Jordi columns are made from 100% divinylbenzene providing maximum column life, improved resolution and more linear calibration curves due to the high pore volume of the Jordi columns. Additional benefits of choosing Jordi columns: Rugged - Jordi s divinylbenzene packing can be run at high temperatures in nearly all solvents. Change solvents without damaging the Jordi column. Most Jordi packings can be used at temperatures of up to 150 C and pressures up to 30,000 psi. ph Resistant - Jordi columns are usable over the entire ph range allowing the Jordi columns to support mobile phase & ph values Most competitive silica-based columns typically operate in the ph 2-7 range. Highly efficient - High plate counts for sharp, symmetrical peaks. Powerful separation tool - High pore-volume for greater resolution with fewer columns, as well as porosities to cover the complete molecular weight range. Jordi columns use a larger standard column dimension of 10 mm (industry norm columns are 7.8 mm) at low cost. Versatile - Largest selection of surface chemistries available, providing the greatest opportunity for a successful separation. Jordi offers a complete line of GPC columns in analytical, semi-prep, and prep sizes. For more information about the Jordi line of products and their separation capabilities, please visit our website or contact your Product Specialist. Cat. # Description Pore Size Part. Size ID x L Jordi Gel DVB Column (MW range = ,000,000 g/mol) Mixed-Bed 5 μm 10 x 250 mm Jordi Gel DVB Flourinated Column (MW range = ,000,000 g/mol) Mixed-Bed 5 μm 10 x 250 mm Jordi Gel DVB Glucose Column (MW range = ,000,000 g/mol) Aqueous GPC Mixed-Bed 5 μm 10 x 250 mm Jordi Gel DVB Reverse Phase Column 300 Å 5 μm 4.6 x 250 mm Jordi Gel DVB Reverse Phase Column 1000 Å 5 μm 4.6 x 250 mm Jordi Gel DVB Reverse Phase C18 Column 500 Å 5 μm 4.6 x 250 mm Jordi Gel NP DVB Glucose Column (MW range = ,000,000 g/mol) 500 Å 5 μm 4.6 x 250 mm For more selections and sizes, visit our website or contact your personal Product Specialist. In addition to Jordi s complete line of polymer-based gel permeation chromatography (GPC) columns, we offer a full line of SPE Cartridges (see SPE Cartridges - Jordi) and Well Plates as well as Bulk Packing Media. All Jordi GPC products are made using their high quality 100% divinylbenzene packing materials which have been in continuous production for over 25 years. Jordi uses only the highest quality resins in all of their GPC products. These products are thoroughly tested to ensure consistency from batch to batch including verification of particle shape, size and purity as well as chromatographic performance. 147

25 Jordi xstream Column Use a single Jordi xstream H 2 O in 100% aqueous, 100% organic or any mobile phase by simply changing solvents. Jordi xstream H 2 O columns represent a breakthrough in GPC column technology. This revolutionary column allows for the broadest range of separations currently obtainable with any GPC column. xstream columns were designed for aqueous GPC separations including the analysis of dextrans and polysaccharides. It performs equally well for the analysis of organic soluble polymers including the analysis of polystyrene or other common polymers in THF or chloroform. It is also an excellent choice for the analysis of nylons and poly(ethyleneterepthalate) in hexafluoroisopropanol (HFIP). A single xstream column can be used for aqueous or organic GPC analysis by simply changing solvents. xstream H 2 O GPC columns are an excellent column for aqueous GPC analyses. This includes the analysis of a wide range of aqueous soluble polymers including polysaccharides such as dextrans and starches. Other polymers which can be analyzed include butyl methacrylate / isobutyl methacrylate copolymer, phenoxy resin, poly(n-butyl methacrylate), polycaprolactone and poly(isobutyl methacrylate). In addition, the presence of salts in the mobile phase can be used for the analysis of cationic polymers such as quaternary amine polymers. The broad solvent compatibility of xstream H 2 O GPC columns allows for their application in all common organic solvents. This includes analyses in polar organic solvents such as DMSO, DMF and DMAC as well as more hydrophobic solvents such as THF and chloroform. Examples of polymer systems which have been analyzed include poly(methyl methacrylate), poly(vinyl chloride) and copolymers of acrylonitrile, styrene, butadiene, butyl methacrylate. Advantages of Jordi xstream H 2 O GPC columns: Separation - Broadest applicability of any currently available GPC column Mw Range - up to 700K molecular weight in H 2 O, up to 10 million molecular weight in organic solvents Solvent Compatibility - water and all common organic solvents Wide Range of Porosities Å to 105 Å, Mixed Bed and SB Rugged - long column life - 3 month warranty standard Quality - subjected to rigorous quality checks Poly(methyl methacrylate) in HFIP Polysaccharides in H2O Color Brown - Methyl Vinyl Ether-Malecacid Copolymer - Pure H 2 O Blue - Polysufone - DMAC Red - Sodium Alginate - H 2 O/MEOH/0.01M EDTA Green - Polyvinyl Chloride - THF Jordi xstream Cat. # Description Pore Size Particle Size ID x L Jordi xstream H 2 O Column 1000 Å 5 μm 10 x 250 mm Jordi xstream H 2 O Column Å 5 μm 10 x 250 mm Jordi xstream H 2 O Column Mixed Bed 5 μm 10 x 250 mm 148

26 Column Packing System Pack your own columns and Save Money SSI Lab Alliance - Pack in a Box The Portable Column Packing System Pack-in-a-Box includes the following: 1. Model CP Constant Pressure Pump, non-flush, ml/min., 10,000 psig, with transducer and RS-232 control, SS, 110/220V 2. Two empty column assemblies, 4.6 x 150 mm (column blanks, column plugs, end fittings, 2 μm frits) 3. Column Packer assembly (20 ml reservoir, nuts, caps, ferrules, column adapter 5/ mm, gasket, tubing) 4. Packer Bracket Assembly (bracket, rods, bolts, knobs, nuts) 5. C18 Spherical Silica Gel, 10 g 6. Tubing, 1/16 OD x ID, Stainless steel 7. Quick-Set pump control software 8. Instructions Complete Pump Control Fast or Pressure limited ramps Ramp speed adjustments Packing pressure Packing Flow Upper and lower pressure limits Cat. # Product Pack-in-a-Box kit Column packer reservoir, 20 ml, 5-3/4 in Column packer reservoir, 60 ml, 15 in Column packer reservoir, 500 ml Column assembly, 2.1 x 150 mm x 2 μm Column assembly, 4.6 x 150 mm x 2 μm Column assembly, 4.6 x 250 mm x 2 μm Affordable and perfect for universities and research labs. Prep Column Packer also available online at NOTE: Pack-in-a-Box has many optional parts to fit a variety of Columns. For more selections and sizes, visit our website or contact your personal Product Specialist. 149