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1 uortig Iormtio: Pti moiitio oirmtio vi 1 MR: j 5 FEFEFKFK FEFEFKFK moii m g i o i o g m l g m l - - h k 3 h k 3 Figur 1: 1 -MR or th umoii moii tis 1 Grou ty Pk [m] Assigmt (ko rst 8.63 oly i umoii ti) 3 Aromti rigs 7.,, (ko) (xt to romti rigs) 3.3 g 6 (xt to si his) h 7 (xt to si his) i 8 DM (xt to rst oly i moii ti).35 j 1 (si his).-. k 11 (si his) 1.-. l, m,, o 1 (ko) Tl 1: 1 MR ks ssig to grous 15 All ks rst i th 1 MR str ( Mz Brukr) or oth th ur moii tis r ssig i Tl 1. Th most rlvt o ths r 8.63 m.35 m. Th k t 8.63 m orrsos to th r grou i th ur, umoii ti. As xt, this grou is rst oly i th origil ti ot i th moii ti. Th grou o th moii ti is th k t.35 m i th moii ti is ttriut to its ighourig grou. Ths rsults suggst th sussul moiitio o th ti. This jourl is Th Royl oity o hmistry [jourl], [yr], [vol], 1
2 Pti utiolistio oirmtio y MALDI-To: FEFEFKFK %It {r3} {r388} {r3} {r388} 11.9 {r3669} {r3669} Figur : MALDI-To o th umoii ti (FEFEFKFK) Mss/hrg FEFEFKFK moii %It {r88} {r51} 137.3{r88} 137.3{r51} {r357} 137.8{r79} {r357} {r85} {r79} Mss/hrg 3 Figur 3: MALDI-To o th moii ti (mo FEFEFKFK) 35 Th rsults giv i igurs 3 show sigiit ir i th molulr wight o th two tis (th moii ti is ~9 g mol -1 hvir tht th umoii ti) oirmig utiolistio. Ths rsults wr oti rom Krtos P Axim FR V...1 ort i rltio mo. [jourl], [yr], [vol], This jourl is Th Royl oity o hmistry [yr]
3 Ptis urity vlutio y PL: mau65 3 %D:. % %:. % Flow: 1. ml/mi 3 FEFEFKFK 8. UV_VI_ WVL:65 m 1 %B:. % Tim / mi Figur : PL o th umoii ti (FEFEFKFK) 5 o. Rt.Tim Ar ight Rl.Ar mi mau*mi mau % Totl Tl : PL rsults or th origil ti th urity rtg This jourl is Th Royl oity o hmistry [jourl], [yr], [vol], 3
4 mau65 3 %D:. % %:. % Flow: 1. ml/mi FEFEFKFK moii 8. UV_VI_ WVL:65 m %B:. % Tim / mi Figur 5: PL o th moii ti (mo FEFEFKFK) o. Rt.Tim Ar ight Rl.Ar mi mau*mi mau % Totl: Tl 3: PL rsults or th moii ti th urity rtg 6 Th PL rsults show high urity i oth th umoii moii tis; 81 % 76 % rstivly, oirmig th MALDI rsults. Ths rsults wr otiig usig rvrs hs PL whr ur A ws PL gr wtr with.1% triluoroti i (TFA) ur B ws PL gr toitril with.1% TFA. A grit o -8% o B ws us with sml ijtio o μl. Th ollowig st u ws mloy: Diox P68 PL um, with Diox AI utomt sml ijtor, Diox UVD17U ttor. Th olum is Mhry-gl E5/.6 ulosil olum. [jourl], [yr], [vol], This jourl is Th Royl oity o hmistry [yr]
5 65 oirmtio o ojugtio y soli stt MR: PiPAAm * * = romti FEFEFKFK φ ) = ) * * * 18 9 m rom TM ojugtdmf 18 9 m rom TM 1 1 ) m rom TM 7 Figur 6: oli stt MR: ) ur olymr, ) ur ti ) th ojugt. 75 Atr sythsis uriitio th ojugt ws lys y soli stt MR usig th oojugt olymr th ti s strs. tr r show i Figur 6 whr ks hv itii. For th ojugt (Figur 6), sulmtry ks wr ou i th rg o m, tht orrsos to th hyl rigs rst i th ti. Ths rsults oirm th rs o th ti i th ojugt strutur. MR str wr ror t 9. T usig Brukr DX- stromtr, i 1 to 13 ross-olristio mgi gl siig mo. This jourl is Th Royl oity o hmistry [jourl], [yr], [vol], 5
6 6 [jourl], [yr], [vol], This jourl is Th Royl oity o hmistry [yr] Qutiitio ti/ipaam rtio i ojugt y 1 MR UV: 8 Figur 7: 1 MR str or ur olymr or th ojugt 85 Figur 8: Mgii 1 MR str or ur olymr or th ojugt PIPAAm 5g/L PFEFEFKFEFK 5g/L m PIPAAm 5g/L PFEFEFKFEFK 5g/L m PFEFEFKFK 5g/L
7 9 As. Itsity /.u ti 1g L -1 ti.9g L -1 ti.7g L -1 ti.5g L -1 ti.3g L -1 As. / u lirtio ourv or ti otrtio / g L -1 ti.1g L -1 ojugt 1g L wvlgth / m Figur 9: UV str or umoii ti solutios with vryig otrtios (ott lis) or ojugt solutio t 1 g L 1. Isrt: lirtio urv (los imos) oti or 95 th umoii ti ojugt sor (o imo). 15 I orr to qutiy th mout o ti tth t th o th olymri hi, two mthos wr us: roto MR UV. Pk ssigmt i th 1 MR str is show i Figur 7 th rgio o itrst or qutiitio is mgii i Figur 8. Th ks t.1,.55.8 m orrso to th rom th ko o th ti r vry istitiv. Ths ks wr us or qutiitio tkig th 3 grous o olymri hi s rr oit. Th 1 MR str wr ror o Brukr, Mz stromtr, usig 16 ss or tim vrgig. Th sor t 58 m ws us to oirm th qutiitio o mout o ti y UV. A lirtio urv ws uil usig th moii ti ws th susqutly us to qutiy th mout o ti rst i th ojugt. tr wr ror o himzu 51 oul m stromtr. This jourl is Th Royl oity o hmistry [jourl], [yr], [vol], 7
8 Grhil ott Etry 3 3 olymr-ti ioojugt 3 3 Tm ~ 75 º Tim, RT Tm. < ~ 3 º < 8 [jourl], [yr], [vol], This jourl is Th Royl oity o hmistry [yr]
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