OER implementation at UNS. Prof. Dr Ivana Ivančev-Tumbas University of Novi Sad, Faculty of Sciences

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1 OER implementation at UNS Prof. Dr Ivana Ivančev-Tumbas University of Novi Sad, Faculty of Sciences

2 Overview 1. Planned development of OER 2. What do we need from EU partners? 3. Draft OER example

3 Overview 1. Planned development of OER 2. What do we need from EU partners? 3. Draft OER example

4 Faculty of Science Topic/level Matrix interferences in the flame atomic absorption spectrofotometry/phd (lab exercise) Method optimization for analysis anion by ion chromatography/phd (lab excercise) GC/MSmethod validation/phd (theoreticalexcercise) Controlcharts preparation for the method of analysis of total organic carbon /MSc(lab exercise) Validation of HPLC/DAD method/phd(lecture) Qualitative analysis using LC-DAD-MS/MS/PhD Topic/level LC-DAD-MS/MS operation /MSc (lab exercise) Removal of organic pollutants from water by means of advanced oxidation processes/msc(lecture) Photocatalyticdegradation of a biologically actives substance/phd (lecture) Isolation of selected natural product from plant material by semi-preparative HPLC Determination of preservatives and other additives in soft beverages by LC-DAD /MSc(lab exercise) Identification and quantification of secondary biomolecules in plant material by LC-DAD-MS/MS/PhD

5 Topic/level Engineered adsorption processes. Green and 'low-cost' technologies used in waste stream treatment/msc Instrumental analysis as a tool required for the quantification of residues of pollutants before and after the treatment of waste streams. Chromatography. Spectrophotometry/MSc Combined techniques (GC-MS, GC-MS/MS, HPLC-MS, HPLC-MS/MS, HPLC-TOF-MS/MS)/PhD, lecture and lab exercise Faculty of Technical Science Topic/level Groundwater monitoring: groundwater sampling technique, measuringofgroundwater level in wells (piezometers), instrumentation in groundwater monitoring, field measurements. Leachatemonitoring: leachatesampling technique, instrumentation in leachatemonitoring, field measurements/msc Sensors techniques in environmental monitoring programmes/msc

6 We have learned that the choices for OER delivery are wide Moodle Quiz based on bank of questions Lectures PPT and/or PREZI Cmaps

7 We still need to learn about sharing and copyrights!

8 Overview 1. Planned development of OER 2. What do we need from EU partners? 3. Draft OER example

9 MATCH of offer from EU partners and PC needs Topic EU Type Reminders in chemistry, kinetics and thermochemistryuseful for understanding the formation and dissociations of ions. Examples of applications in iii) environment Validation issues in bioanalysis Metabolomics principle and application Analysis of Protein post-translational modifications by mass spectrometry NMR video Methodsfor isolation of target analytes(spe, PSE, SPME, UAE, MAE etc.); methods of extracts clean-up and group fractionation (column chromatography, gel permeation chromatography); derivatizationfor GC analysis; GC-FID, GC-ECD, liquid chromatography with UV-VIS, Interpretation of EI and soft ionization mass spectra, comprehensive two-dimensional gas chromatography with mass spectrometric detection J.C-T. J.C-T. E.E/F.F. M.A J.C Real ex. Real ex. Videoof procedures Courses in professional insertion A.B. CPD? Proposal for joint CPD with UNS?

10 Overview 1. Planned development of OER 2. What do we need from EU partners? 3. Draft OER example

11 Environmental Quality Control (Advanced Course), PhD 1. GC/MS method validation, theoretical excercise, by I. Ivančev- Tumbas 2. Matrix interferences in the flame atomic absorption spectrofotometry by S. Maletić and I. Ivančev-Tumbas 3. Method optimization for analysis of anions by ion chromatography by S. Maletić, M. Watson and I. Ivančev-Tumbas

12 Example of GC/MS method validation (OER 1) Consists of several education elements: Entrance test with references and answers Concept map for Development of own Method validation plan Pictures Video-clips Word documents Real example test Are those parts separate elements?

13 Entrance test Before student can think of own method he/she has to have knowledge gained at previous levels of studies. To chek, repeat and prepare oneself he/she will do: Quiz with answers and direction to literature references To check understanding, application, analysis of the process in instrument This has to be done before start of the method development plan Will be on Moodle platform

14 Question data base has to be made for topics, appropriate for the highest level of education: 1. How GC works Parts Principle Retention times Columns Injectors (liners, septa, peak focusing) Detectors 2. How MS works EI, CI Data aquisition SIM, SCAN Data analysis- qualitative and quantitative analysis (IS, external standars, standard addition) Check is it working in a proper way 3. Sample prep Extraction Clean up

15 Examples of questions Instead of the question: What is the retention time? they will be asked to read and compare RT from real chromatograms. How is RT influenced by the temperature and or gas flow? they will be asked to explain why some changes in real chromatograms happen due to temperature and flow changes. To explain CI and EI they will be asked to gues which one type of ionisation is used to get certain spectra and how they can increase sensitivity of their instrument.... Thus we will encourage both analytical and synthetical way of thinking as well as evaluation before they get main task.

18 Concept map-synthesis of the knowledge

19 Concept map-doc, jpeg and clips will be added

20 12/8/2017 Meeting in Belgrade, 5th-8th December 2017

21 Real, problem based example-test on their own substance Evaluation of the developed method for benzophenone analysis in water Internal standard method, benzophenone d-5 Extraction by SPE procedure, OASIS HLB 60mg Evaporation to dryness and derivatisation with PFBHA The results of the testing method for benzophenone analysis are given in Test excell file with four sheets: Calibration, Blank LOD LOQ, Precision, Recovery.

22 Calibration Worksheet Calibration contains the surfaces of target ions peaks for benzophenone (BP), m/z 377 and IS, (BP-d 5 ), m/z 382. Calibration has been done 3 times for concentration levels of 20, 100, 200, 400, 750 and 1100 ng/l. Concentration of IS was 500 ng/l. Task: present your plan of making solutions if you want to do SPE with 200 ml of water. Task: Calculate average linear equation for calibration curve and coresponding coefficient of correlation.

24 LOD and LOQ In worksheet named Blank LODLOQ data for 7 measurements of blank samples (water matrix spiked with internal standard in same concentration as calibration standards) are given. Task: Calculate LOD and LOQ

25 Precision Instrument precision was checked by injecting calibration standard at 6th concentration level (1100 ng/l) three times from the same vial. Task: report on precision of those injections by analysing responses of the detector for target ion of BP Method precision was checked by analysing injections in triplicate for two different concentrations within calibration curve (e.g. At 200 and 750 ng/l level). Task: report on precision of those injections by analysing ratio of responses of the detector for target ion if BP and target ion of IS for triplicate measruerments at each concentration level

27 Recovery test flow Spiked clean water matrix and addition of IS SPE Collection into clean vial Evaporation to dryness Derivatisation Injectio n Clean water matrix SPE Collection into vial with added IS and compound in same quantity and dried before collection of extract Evaporation to dryness Derivatisation Injection Results of testing are presented in worksheet recovery. Task: calculate the values for recovery and elaborate are they satisfactory or not and why.

28 Example at low concentration ion RT RLW1 A/Ais RLW2 A/Ais RLW3 A/Ais RSPEL1 A/Ais RSPEL2 A/Ais RSPEL3 A/Ais BP Dbp ng/l RL1 RL2 RL3 RL4 RL5 RL6 RL7 RL8 RL9 av sd RSD % % RLW1/RSPEL1

29 By calculation of R value student will obtain erroneous results of 300% and 800% He or she has to conclude why is it so and to design the action to propose the action to solve the issue.

30 Recovery test flow Spiked clean water matrix and addition of IS SPE Collection into clean vial Evaporation to dryness Derivatisation Injectio n Clean water matrix SPE Collection into vial with added IS and compound in same quantity and dried before collection of extract Evaporation to dryness Derivatisation Injection

31 Thank you for the cooperation

Application Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors

Application Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors Trace-Level Analysis of Melamine in Milk Products on Agilent 789A/5975C GC/MSD Using a ew Agilent J&W DB-5ms Ultra Inert Column and SampliQ SCX Cartridges Application ote Gas Chromatography/Mass Spectrometry/Food