Liquid Chromatograph Mass Spectrometer LCMS-8040 C146-E188

Size: px
Start display at page:

Download "Liquid Chromatograph Mass Spectrometer LCMS-8040 C146-E188"

Transcription

1 Liquid Chromatograph Mass Spectrometer C146-E188

2

3 Ultra-High Sensitivity By incorporating newly improved ion optics and collision cell technology, the provides higher multiple reaction monitoring (MRM) sensitivity. A five-fold increase in sensitivity (reserpine, S/N ratio) has been achieved by improving ion focusing and minimizing ion losses between multi-pole lenses. These improvements also yield higher sensitivity for scan mode measurements. This higher sensitivity expands the potential range of LC/MS/MS applications. Ultra-High Speed The was designed to provide significantly higher sensitivity while maintaining the high speed offered by the LCMS-83. Ultrafast MRM transition speeds, up to 555 MRMs per second, are achieved by Shimadzu s UFsweeper collision cell technology, proprietary high-precision quadrupole machining capabilities, and unique high voltage power supply technology. In addition, the features the world s fastest* polarity switching at 15 msec. With this high-speed performance, the can dramatically improve analytical throughput. Ultra-High Reliability MRM optimization in Shimadzu s LCMS systems is based on a rapid series of automated flow injection analyses, requiring only minutes to perform. Multiple compounds can be optimized in an unattended sequence, freeing the analyst from tedious work. MRM parameters optimized for the LCMS-83 can be transferred to the, making it possible to transfer methods between systems. The offers the same ease of maintenance benefits as the LCMS-83, and all consumables, such as desolvation lines (DL) and ESI capillaries, are interchangeable as well. * Per survey result as of May 212 GCMS-QP21 Ultra GCMS-QP21 SE GCMS-TQ83 LCMS-83 LCMS-88 LCMS-22 LCMS-IT-TOF

4 Ultra-High Sensitivity with UFMS Technology Higher Sensitivity with Improved Ion Optics UF-Lens TM The UF-Lens is a lens system that offers both higher sensitivity and easier maintenance. The optical system integrates two multi-pole RF ion guides for higher sensitivity. In addition, the lens system can be removed without tools for easy cleaning. Sprayer UF-Lens Qarray Multi Pole 1 Q1 Pre-rod Q1 Post-rod Q3 Pre-rod Q1 Q3 Atmospheric Pressure Desolvation Line Skimmer Multi Pole 2 UFsweeper Detector Collision Cell 1 st Vacuum Chamber 2 nd 3 rd 4 th RP Triple Inlets Tmp 4 Stage Differential Pumping System Ion losses between segments are minimized by utilizing quadrupole ion guides. Previous Configuration UF-Lens TM LCMS-83 A comparison of auto-tune Q1 scan results for a standard sample (a mixture of PEG, PPG, and raffinose) obtained from the LCMS-83 and is shown to the right. Target ions used for ESI+ auto-tuning are plotted (m/z 65.5, 168.1, , 344.2, 652.4, 14.6, and ). A significant sensitivity increase across a broad molecular weight range for precursor ion transmission is demonstrated. Increase in Sensitivity (83 vs. 84) m/z Speed Beyond Comparison Enhanced Sensitivity

5 Higher CID Efficiency with Improved Collision Cell UFsweeper TM The UFsweeper II is a high-sensitivity, high-speed collision cell that features improved ion focusing by using high-speed ion transport technology. This yields better product ion transmission in the collision cell, maintaining signal intensity and suppressing crosstalk, even for high-speed or simultaneous multi-component analysis. The capability for high-throughput analysis is thus maintained at lower levels of detection. Sample Name Precursor m/z Product m/z LCMS-83 CID efficiency CID efficiency Proportional Increase Lidocaine Atropine Yohimbine Tetracaine Doxepin Imipramine Nortriptyline Isopropylantipyrine Diazepam Reserpine The offers higher sensitivity while maintaining the ultrafast performance of the LCMS-83. The figures below show simultaneous analysis of 167 pesticides in MRM positive/negative ion analysis mode. Even though positive and negative ions were LCMS ( 1,,) measured simultaneously, both the LCMS-83 and accurately identified all 167 components. In addition, the showed improved sensitivity an average of three times higher for all components. 1. ( 1,,) min min HPLC Analysis Column Mobile Phase A Mobile Phase B Gradient Program Flow Rate Column Temperature Shim-pack FC-ODS (2. mmi.d. 15 mml., 3µm) 5 mmol/l ammonium acetate - Water 5 mmol/l ammonium acetate - Methanol 15 %B ( min) 4 %B (1 3.5 min) 5 %B (6 min) 55 %B (8 min) 95 %B ( min) 15 %B (3.1 4 min).2 ml/min 4 C MS Probe Voltage Nebulizing Gas Flow Drying Gas Flow DL Temperature Heat Block Temperature +4.5 kv (ESI-Positive mode) / 3.5 kv (ESI-Negative mode) L/min 1 L/min 25 C 4 C Liquid Chromatograph Mass Spectrometer 5

6 Proven Ultra-High Performance now with Enhanced Sensitivity Higher Sensitivity for Q1 Full Scan Spectra The maintains the same high-speed scanning (UFscanning) and polarity switching technology (UFswitching) utilized in the LCMS-83. The not only maintains Shimadzu's proprietary high-speed technologies (UF Technologies, Patent pending), which minimize sensitivity losses even at faster scan speeds, it also features improved ion optics, which provide higher sensitivity for MRMs and full scans. A comparison of Q1 full scan spectra for pesticide samples (Methomyl, Carbaryl(NAC), Phoxim, Benfuracarb, and Abamectin B1a) is shown below. The upper spectra were acquired using the and the lower spectra were acquired using the LCMS-83. As shown, the offers significant sensitivity improvements for precursor ions or full scan data. Inten.( 1,,) Inten.( 1,,) Inten.( 1,,) m/z 15 u/sec LCMS-83 Inten.( 1,,) m/z 1 u/sec Inten.( 1,,) m/z 5 u/sec Inten.( 1,,) m/z 15 u/sec m/z 1 u/sec m/z 5 u/sec 1..5 Higher Sensitivity for MS/MS Acquisition Modes The increased scan sensitivity of the also applies to scan modes specific to triple quadrupole mass spectrometers, such as product ion scanning, precursor ion scanning, and neutral loss scanning. Historically, mass spectrometers have introduced mass deviation in linked scans, such as precursor ion scans or neutral loss scans, when measured at maximum scan speeds. Inten.( 1 5 ) Inten.( 1 7 ) u/sec Precursor ion of m/z m/z Inten.( 1 5 ) m/z Inten.( 1 7 ) u/sec Dipropyl phthalate CH 3 m/z CH min min Precursor Ion Scan of Standard Mixture Sample of 8 Phthalate Esters Mass error associated with a rise in scan speed Increase in Scan Speed m/z Typical triple quadrupole mass spectrometer However, Shimadzu's proprietary UFscanning technology allows performing precursor ion scans or neutral loss scans at high speeds without loss of mass accuracy. In addition, the offers higher sensitivity levels. Precursor scan results for eight kinds of phthalate esters are shown to the left. Scans were performed at two speeds, 2727 u/sec and 6 u/sec. No mass shift is observed at either scan speed, and a significant sensitivity improvement is observed for the. 6 Speed Beyond Comparison Enhanced Sensitivity

7 Proven Interface for Robust Performance Robust, User-Friendly Interface The LCMS-83 and use the same interface. In LCMS analysis, it is necessary to adjust interface conditions such as temperatures and gas flow rates for optimal desolvation. Spray needle adjustment and probe position are also factors to be evaluated. In both the LCMS-83 and, temperatures and flows are easily controlled through the software. ESI probe position is set by a single easily accessible knob, and spray needle protrusion is adjusted without the need for any special tools or disassembly of the probe. In addition, this capillary incorporates a tapered design, which reduces sample clogging. Signal-to-noise test results are shown right. Sensitivity specifications (S/N > 1 Central position Locking screw Position adjustment knob 2mm +3mm Attach/detach with a single flip of a lever Integrated ESI Capillary for the and S/N > 2 for the LCMS-83) were satisfactorily obtained at each of four probe positions (from 1mm to +2mm), demonstrating the interface s robustness. ( 1,) min S/N: 172 LCMS-83 ( 1,) min S/N: 191 ( 1,) min S/N: 148 ( 1,) min S/N: 895 ( 1,) min S/N: 147 ( 1,) min S/N: 286 ( 1,) min S/N: 217 ( 1,) min S/N: 1315 S/N test data for 1 pg reserpine FIA (upper: and lower: LCMS-83) measured using fixed parameters (ESI+, 69.3 > 195., CE: -37, dwell: 197 msec, and pause: 3 msec) after auto-tuning. Easy Method Transfer MRM parameters for the LCMS-83 can be used in systems. Optimization of MRM parameters is an early step in LCMS method development. Shimadzu has streamlined the MRM optimization process with a rapid and simplified approach based on automated flow injection analysis. Several method packages, which contain chromatographic and optimized MRM conditions for a variety of analytes, including residual pesticides, veterinary drugs, and forensic drugs of abuse, have also been released. Laboratories employing LCMS-83 MRM conditions will be able to transfer these MRMs directly to the. To demonstrate this, a method for the simultaneous analysis of 167 pesticides was transferred without modification from the LCMS-83 to the. Increased sensitivity was obtained for all compounds; three example chromatograms are shown below. 1 ppb: Cloquintocet-mexyl(9.5X) 1 ppb: Pyrazosulfuron-ethyl(7.4X) 1 ppb: Linuron(5.2X) ( 1,) ( 1) ( 1,) ESI > ESI >182.1 ESI > min min min Liquid Chromatograph Mass Spectrometer 7

8 Ultra-High Reliability to Withstand Wide Range of Complex Matrices Exceptional Durability Blood plasma samples were spiked with verapamil and warfarin, and then deproteinized according to the pretreatment process indicated below. The area values from 45 consecutive analyses were then plotted. Simultaneous analysis of verapamil by ESI+ and warfarin by ESI was performed. Chromatograms for the 1st, 25th, and 45th measurements are shown below. This resulted in 1 pg on-column area repeatability of 4.18 % for verapamil and 6.61 % for warfarin. Area Verapamil Warfarin Column: Shim-pack XR-ODS II (2. mmi.d. 5 mml, 2.2 µm) Mobile Phase A: 5 mmol/l ammonium acetate water Mobile Phase B: Acetonitrile Gradient Program: 6 %B ( min) 9 %B (1 3. min) 6 %B ( min) Flow Rate:.4 ml/min Injection 2 µl plasma sample Add 2 µl Acetonitrile, 5 µl 5 % methanol aqueous solution and 5 µl verapamil and warfarin standard solutions. Vortex and centrifuge (1 rpm, 3 min.) followed by freeze drying. Add 5 µl dilution solution. Vortex and centrifuge (12 rpm, 5 min.). ( 1,) ( 1,) ( 1,) 1 st injection th injection th injection min min min Ultra Fast Speed Combined with Lower Femtogram Detection Using the same chromatography parameters as described above, analysis of plasma samples spiked with 4 fg/µl verapamil and warfarin were injected into the for signal to noise determination. For 1 fg on-column, a S/N ratio of 146 (rms) was obtained for verapamil and 3 (rms) for warfarin. The resulting lower limits of detection for S/N = 3 (rms) were 2.5 fg for verapamil and 9.88 fg for warfarin Verapamil Warfarin >165.5(+) >16(-) Compounds Verapamil Warfarin S/N: 1 fg on column (rms) LOD(fg) Calculated at S/N = min min Chromatograms of 1 fg Verapamil and Warfarin On-Column 8 Speed Beyond Comparison Enhanced Sensitivity

9 Single Vendor Solution Provides Seamless Operation LabSolutions LCMS Version 5 LabSolutions LCMS Version 5 is integrated workstation software used to control LCMS-22/83/84 systems. Full HPLC control is included for LC-VP and Prominence modules, and for the Nexera UHPLC series. Standard data processing features include a Quantitation Browser, which is useful for quantitative processing of multiple analytes, and a Data Browser for overlaying chromatograms. The Data Browser allows the user to analyze and compare multiple sets of data, such as peak detection data or LCMS spectra in the same window. It also allows LC and MS data to be linked, which is especially useful when searching for or identifying compounds. In addition, the popular report function was expanded to enable printing results in a wider variety of formats. Automatic MRM Optimization Optimizing MRM parameters is an important step when creating quantitative methods for triple quadrupole mass spectrometers. LCMS-83/84 systems optimize MRM parameters using automated flow injection analysis, an optimization function included LabSolutions that allows anyone to optimize parameters easily. Entering precursor ions Selecting parameters to optimize Unknown product ions Optimization results Optimization choices include voltages for Q1 pre-rod, Q3 pre-rod, and Collision Energy. CE optimization finds the optimal value by first searching a wide range at 5 volt steps, then searching again within a ±5 V range around the highest intensity value at 1 volt steps. MRM transitions are determined by selecting the product ions with the highest abundance. This entire series of measurements is performed automatically and a number of analytes can be optimized in an unattended batch run. Optimization results are automatically reflected in method files, and shown in the window above. Values indicated as "Selected" are the selected product ions. The user can easily make edits to the final method. If a product ion is unknown, the software automatically searches for the ion. Liquid Chromatograph Mass Spectrometer 9

10 Powerful Workflow Solutions LC/MS/MS Method Packages When performing quantitation, system parameters must first be optimized. Shimadzu offers various method packages to eliminate the need for MRM determination and chromatographic method development. Residual Pesticides No. of compounds: 167 Veterinary Drugs No. of compounds: 42 Water Quality Analysis No. of compounds: 44 (Golf Course Pesticides), 32 (Pesticides Targeted for Water Quality Control) Ready-to-Use Methods Shimadzu s method packages contain LabSolutions method files, which include pre-registered MRM parameters with optimized quantitative and reference ions, LC separation parameters, and retention times and peak identification parameters for various compounds. Report templates for outputting quantitation results are also included. By using these method packages, a laboratory can save a great deal of method development time. Methods Easily Customized LabSolutions LCMS Version 5 now allows the transfer of individual MRMs from one method to another. Adding, deleting, and editing of compounds is easily accomplished in an intuitive format. Method packages also include a compound parameter list created in Excel, which enables the creation of new tables that list only the components needed. LC/MS/MS Method Package for Drugs of Abuse: 286 compounds The drugs of abuse method package was developed for both quantitative and qualitative drug screening analysis. The speed of Shimadzu s LCMS allows product ion scans for qualitative confirmation to be employed during MRM quantitation. Shimadzu refers to the use of an MRM response to trigger a product ion scan as Synchronized Survey Scanning (SSS). This method package includes SSS methods for 87 illicit drugs, 15 psychotropics, 7 hypnotics, and 24 other substances, based on input from researchers. In addition, it includes a library populated with compound information and product ion scan spectra for a total of 286 compounds. MRM parameters and product ion spectra measurement parameters have been optimized for each compound. Finally, because of the ease with which methods may be edited, new analytical methods for a shorter list of compounds can easily be generated. ( 1,) Injection Illicit drugs min MRM chromatograms Psychotropics Hypnotics 1 Compounds in Digest Inten.( 1,) Zolpidem CH3 N CH3 H3C N N O CH3 1. Other Method selection 221 Illicit drugs Psychotropics Hypnotics 1 digest Other m/z MS/MS spectrum 1 Speed Beyond Comparison Enhanced Sensitivity

11 Profiling Solution Version 1.1 Compatible with LCMS-IT-TOF, LCMS-22/83/84, and GC-MS Data Data File Profiling Solution is software for comparing and contrasting the vast amount of data obtained from multiple chromatographic sets. It extracts and tabulates all peaks found in multiple data files to create the peak tables required for multivariate analysis. Furthermore, Profiling Solution is compatible with both LC-MS and GC-MS instruments, which allows supporting multivariate analysis using a wide variety of analytical methods. Detected Peak Analytical Instrument Multiple Data Data Table Multivariate Analysis Software Optional Ion Source Changing between ESI, APCI and DUIS interface is a completely tool-free operation. APCI DUIS Sample injection ESI probe Corona needle Corona needle Liquid Chromatograph Mass Spectrometer 11

12 Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol TM or. Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own. For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you as is without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. Shimadzu Corporation, 212 Printed in Japan ANS

Liquid Chromatograph Mass Spectrometer LCMS-8040 C146-E188A

Liquid Chromatograph Mass Spectrometer LCMS-8040 C146-E188A Liquid Chromatograph Mass Spectrometer C146-E188A Ultra-High Sensitivity By incorporating newly improved ion optics and collision cell technology, the provides higher multiple reaction monitoring (MRM)

More information

Quadrupole Time-of-Flight Liquid Chromatograph Mass Spectrometer LCMS-9030 C146-E365

Quadrupole Time-of-Flight Liquid Chromatograph Mass Spectrometer LCMS-9030 C146-E365 Quadrupole Time-of-Flight Liquid Chromatograph Mass Spectrometer LCMS-9030 C146-E365 Effortless Performance The LCMS-9030 quadrupole time-of-flight (Q-TOF) mass spectrometer integrates the world s fastest

More information

Simultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS

Simultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS Simultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS TIAFT 204 Toshikazu Minohata, Keiko Kudo 2, Kiyotaka Usui 3, Noriaki Shima 4, Munehiro Katagi 4, Hitoshi

More information

High-sensitivity, high-throughput quantitation of catecholamine metabolites in urine by LC/MS/MS for clinical research

High-sensitivity, high-throughput quantitation of catecholamine metabolites in urine by LC/MS/MS for clinical research PO-CON1655E High-sensitivity, high-throughput quantitation of catecholamine metabolites in urine by LC/MS/MS for clinical research ASMS 2016 MP-079 Atsuhiko TOYAMA 1, Kumie SATOU 2, Yuki NAKAMURA 2, Ichiro

More information

Multi-residue analysis of pesticides in crude food extracts using a simple extraction technique and LC/MS/MS

Multi-residue analysis of pesticides in crude food extracts using a simple extraction technique and LC/MS/MS PO-CON1529E Multi-residue analysis of pesticides in crude food extracts using a simple extraction technique and LC/MS/MS ASMS 215 WP 77 Miho Kawashima 1, Yuka Fujito 2, Yayoi Ichiki 3, Miho Sakai 4, Takashi

More information

Screening of pesticides in water using SPE on line

Screening of pesticides in water using SPE on line PO-CON E Screening of pesticides in water using SPE on line HPLC 2013 SAMP-38 Stefano Zaza 1, Stefano Maria Lucini 1 1 Shimadzu Corporation, Milano, Italy 1. Introduction Pesticides are widely used in

More information

Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS

Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Application Note Clinical Research Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Authors Yanan Yang 1, Victor Mandragon 2, and Peter Stone 1 1

More information

Highly sensitive and rapid analysis of synthetic dyes in sea food by LC/MS/MS

Highly sensitive and rapid analysis of synthetic dyes in sea food by LC/MS/MS PO-CON1745E Highly sensitive and rapid analysis of synthetic dyes in sea food by LC/MS/MS ASMS 2017 MP 189 Shailendra Rane 1, Ashutosh Shelar 1, Shailesh Damale 1, Rashi Kochhar 1, Purshottam Sutar 1,

More information

Determination of N-Nitrosamines by USEPA Method 521 using Triple Quadrupole Gas Chromatography Mass Spectrometry

Determination of N-Nitrosamines by USEPA Method 521 using Triple Quadrupole Gas Chromatography Mass Spectrometry PO-CON111E Determination of N-Nitrosamines by USEPA Method 521 using Triple Quadrupole Gas Chromatography Mass Spectrometry Pittcon 201 1120-2 Brahm Prakash, William Lipps, Di Wang, Shilpi Chopra, Nicole

More information

Gas Chromatograph Mass Spectrometer GCMS-TQ8040 C146-E251

Gas Chromatograph Mass Spectrometer GCMS-TQ8040 C146-E251 Gas Chromatograph Mass Spectrometer GCMS-TQ8040 C146-E251 with Smart Technologies Smart Productivity, Smart Operation, Smart Performance Finally, a triple quadrupole GCMS Smart enough for everyday use

More information

Determination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry

Determination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry PO-CON1619E Determination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry Pittcon 2016 480-25 Yan Wang, Feifei Tian, Yueqi Li, Guixiang Yang, Taohong

More information

Gas Chromatograph Mass Spectrometer GCMS-TQ8050 C146-E326

Gas Chromatograph Mass Spectrometer GCMS-TQ8050 C146-E326 Gas Chromatograph Mass Spectrometer GCMS-TQ8050 C146-E326 with Smart Abilities Enhanced Sensitivity Leads to New GC-MS/MS Possibilities The GCMS-TQ8050 features a new highly efficient detector and three

More information

Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis

Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis PO-CON1768E Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide ASMS 2017 TP-194 David Baker 1, Christopher Titman 1, Neil Loftus 1, Jonathan Horner

More information

Highly sensitive quantitative analysis of Felodipine and Hydrochlorothiazide from plasma using LC/MS/MS

Highly sensitive quantitative analysis of Felodipine and Hydrochlorothiazide from plasma using LC/MS/MS PO-CON18E Highly sensitive quantitative analysis of Felodipine and Hydrochlorothiazide from plasma using LC/MS/MS ASMS 201 TP97 Shailendra Rane, Rashi Kochhar, Deepti Bhandarkar, Shruti Raju, Shailesh

More information

Highly sensitive quantitative estimation of genotoxic impurities from API and drug formulation using LC/MS/MS

Highly sensitive quantitative estimation of genotoxic impurities from API and drug formulation using LC/MS/MS P-CN467E Highly sensitive quantitative estimation of genotoxic impurities from API and drug formulation using LC/MS/MS ASMS 24 TP496 Shruti Raju, Deepti Bhandarkar, Rashi Kochhar, Shailesh Damale, Shailendra

More information

Ultra-fast screening of pesticides in foods and agricultural products with desorption corona beam ionization (DCBI) tandem mass spectrometry

Ultra-fast screening of pesticides in foods and agricultural products with desorption corona beam ionization (DCBI) tandem mass spectrometry PO-CON18E Ultra-fast screening of pesticides in foods and agricultural products with desorption corona beam ionization (DCBI) ASMS 21 TP 3 Jing Dong, Satoshi Yamaki, Yuki Hashi, Naoki Hamada Shimadzu China,

More information

Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System

Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Application Note Authors Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon, and

More information

Yun W. Alelyunas, Mark D. Wrona, Russell J. Mortishire-Smith, Nick Tomczyk, and Paul D. Rainville Waters Corporation, Milford, MA, USA INTRODUCTION

Yun W. Alelyunas, Mark D. Wrona, Russell J. Mortishire-Smith, Nick Tomczyk, and Paul D. Rainville Waters Corporation, Milford, MA, USA INTRODUCTION Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma Yun W. Alelyunas, Mark

More information

A novel approach to the analysis of multivitamin by online supercritical fluid extraction/supercritical fluid chromatography

A novel approach to the analysis of multivitamin by online supercritical fluid extraction/supercritical fluid chromatography PO-CON1618E A novel approach to the analysis of multivitamin by online supercritical fluid extraction/supercritical fluid chromatography Pittcon 216 83-12 Qiang Li, Hongyuan Hao, Taohong Huang, Shin-ichi

More information

Overview. Introduction. André Schreiber AB SCIEX Concord, Ontario (Canada)

Overview. Introduction. André Schreiber AB SCIEX Concord, Ontario (Canada) Quantitation and Identification of Pharmaceuticals and Personal Care Products (PPCP) in Environmental Samples using Advanced TripleTOF MS/MS Technology André Schreiber AB SCIEX Concord, Ontario (Canada)

More information

C146-E209. Headspace Samplers. HS-20 Series

C146-E209. Headspace Samplers. HS-20 Series C146-E209 Headspace Samplers HS-20 Series HS-20 Series of Headspace Samplers A Revolutionary System Aimed at Performance and Ease of Use The HS-20 Series is the optimal solution for volatile component

More information

All Ions MS/MS: Targeted Screening and Quantitation Using Agilent TOF and Q-TOF LC/MS Systems

All Ions MS/MS: Targeted Screening and Quantitation Using Agilent TOF and Q-TOF LC/MS Systems All Ions MS/MS: Targeted Screening and Quantitation Using Agilent TOF and Q-TOF LC/MS Systems Technical Overview Introduction All Ions MS/MS is a technique that is available for Agilent high resolution

More information

Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS

Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS Application Note Drug Discovery Authors Srividya Kailasam Agilent

More information

AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching Scores by Removal of Isobaric Interferences

AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching Scores by Removal of Isobaric Interferences AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching s by Removal of Isobaric Interferences Differential Mobility Used as a Tool to Address Selectivity Challenges Adrian M. Taylor

More information

Finnigan LCQ Advantage MAX

Finnigan LCQ Advantage MAX www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Finnigan LCQ Advantage MAX The Finnigan LCQ Advantage MAX ion trap mass spectrometer

More information

Determination of Organochlorine Pesticides and Polychlorinated Biphenyls Using GC/MS/MS Operated in the MRM Mode

Determination of Organochlorine Pesticides and Polychlorinated Biphenyls Using GC/MS/MS Operated in the MRM Mode PO-CON1610E Determination of Organochlorine Pesticides and Polychlorinated Biphenyls Using GC/MS/MS Operated in the MRM Mode Pittcon 2016 1110-2 Brahm Prakash, William Lipps, Di Wang, Shilpi Chopra, Nicole

More information

Translational Biomarker Core

Translational Biomarker Core Translational Biomarker Core Instrumentation Thermo Scientific TSQ Quantum Triple Quadrupole Mass Spectrometers. There are two TSQ Quantum Ultra AM instruments available in the TBC. The TSQ Quantum Ultra

More information

Research of Polychlorinated Biphenyls (PCBs) in Vegetables by GC-MS/MS

Research of Polychlorinated Biphenyls (PCBs) in Vegetables by GC-MS/MS PO-CONE Research of Polychlorinated Biphenyls (PCBs) Pittcon 0 90-8 Xizhi Wang, Shiheng Luo, Feifei Tian, Jun Fan, Guixiang Yang, Taohong Huang, Shin-ichi Kawano, Yuki Hashi Shimadzu Global COE, Shimadzu

More information

Determination of VOCs by USEPA Method 8260 with Extended Dynamic Range using Fast, Sensitive Capillary GC/MS

Determination of VOCs by USEPA Method 8260 with Extended Dynamic Range using Fast, Sensitive Capillary GC/MS PO-CON1609E Determination of VOCs by USEPA Method 8260 with Extended Dynamic Range using Fast, Sensitive Capillary GC/MS Pittcon 2016 1110-1 Brahm Prakash, William Lipps, Di Wang, Shilpi Chopra, Nicole

More information

Thermo Finnigan LTQ. Specifications

Thermo Finnigan LTQ. Specifications IET International Equipment Trading Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Thermo Finnigan LTQ Specifications

More information

Analysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS. No. GCMS No. SSI-GCMS-1401

Analysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS. No. GCMS No. SSI-GCMS-1401 Gas Chromatograph Mass Spectrometer No. GCMS-1401 Analysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS Introduction Development of methods for analysis of drugs of abuse has become a high

More information

New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses

New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses Technical Overview Authors Abstract Peter Stone, Thomas Glauner, Frank Kuhlmann, Tim Schlabach and Ken Miller

More information

Taking Full Advantage of UHPLC with Agilent 6460A Triple Quadrupole MS. Outline

Taking Full Advantage of UHPLC with Agilent 6460A Triple Quadrupole MS. Outline Taking Full Advantage of UHPLC with Agilent 0A Triple Quadrupole MS Dr. Anabel Fandino R&D Application Scientist LC/MS Division Agilent Technologies Santa Clara, CA utline Ultra High Definition LC/MS using

More information

MS n Analysis With Fast Polarity Switching in the Agilent 1100 Series LC/MSD Trap SL. Application Note. Christine Miller Agilent Technologies

MS n Analysis With Fast Polarity Switching in the Agilent 1100 Series LC/MSD Trap SL. Application Note. Christine Miller Agilent Technologies MS n Analysis With Fast Polarity Switching in the Agilent Series LC/MSD Trap SL Application Note Christine Miller Introduction During mass spectrometric analysis, many compounds respond better to a particular

More information

Application News. No. C154. Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis

Application News. No. C154. Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis LAAN-A-LM-E124 Application Liquid Chromatography Mass Spectrometry Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis David R. Baker

More information

SEAMLESS INTEGRATION OF MASS DETECTION INTO THE UV CHROMATOGRAPHIC WORKFLOW

SEAMLESS INTEGRATION OF MASS DETECTION INTO THE UV CHROMATOGRAPHIC WORKFLOW SEAMLESS INTEGRATION OF MASS DETECTION INTO THE UV CHROMATOGRAPHIC WORKFLOW Paula Hong, John Van Antwerp, and Patricia McConville Waters Corporation, Milford, MA, USA Historically UV detection has been

More information

Confirmation of In Vitro Nefazodone Metabolites using the Superior Fragmentation of the QTRAP 5500 LC/MS/MS System

Confirmation of In Vitro Nefazodone Metabolites using the Superior Fragmentation of the QTRAP 5500 LC/MS/MS System Confirmation of In Vitro Nefazodone Metabolites using the Superior Fragmentation of the QTRAP 5500 LC/MS/MS System Claire Bramwell-German, Elliott Jones and Daniel Lebre AB SCIEX, Foster City, California

More information

Agilent ESI and APCI sources: for polar to non-polar compounds

Agilent ESI and APCI sources: for polar to non-polar compounds 1 Agilent 6400 Series Triple Quadrupole Users Workshop 1 Agilent ESI and APCI sources: for polar to non-polar compounds Nebulizer Pressure Corona current Nebulizer Pressure Vaporizer Vcap Vcap Drying Gas

More information

Theory and Practical Consequences.

Theory and Practical Consequences. Theory and Practical Consequences. Number of hits depending on reachable mass accuracy 250 hits @ ±10 ppm 137 hits @ ±5 ppm 78 hits @ ±3 ppm 29 hits @ ±1 ppm 3 hits @ ±0.1 ppm 1 hit @ ± 0.05 ppm Intens.

More information

LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode

LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode Application Note Authors Siji Joseph Agilent Technologies India Pvt. Ltd.

More information

Introducing the Agilent 7000A QQQ-MS for GC Sunil Kulkarni Product Specialist Agilent Technologies

Introducing the Agilent 7000A QQQ-MS for GC Sunil Kulkarni Product Specialist Agilent Technologies Introducing the Agilent 7000A QQQ-MS for GC Sunil Kulkarni Product Specialist Agilent Technologies Page 1 Agilent GC/MS Portfolio Smallest footprint, single GC column 5975VL with 6850GC Industry leading

More information

Multi-residue analysis of pesticides by GC-HRMS

Multi-residue analysis of pesticides by GC-HRMS An Executive Summary Multi-residue analysis of pesticides by GC-HRMS Dr. Hans Mol is senior scientist at RIKILT- Wageningen UR Introduction Regulatory authorities throughout the world set and enforce strict

More information

Thermo Scientific LTQ Orbitrap Velos Hybrid FT Mass Spectrometer

Thermo Scientific LTQ Orbitrap Velos Hybrid FT Mass Spectrometer IET International Equipment Trading Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Thermo Scientific LTQ Orbitrap Velos

More information

Guide to Peptide Quantitation. Agilent clinical research

Guide to Peptide Quantitation. Agilent clinical research Guide to Peptide Quantitation Agilent clinical research Peptide Quantitation for the Clinical Research Laboratory Peptide quantitation is rapidly growing in clinical research as scientists are translating

More information

Agilent 6400 Series Triple Quadrupole LC/MS/MS Users Session

Agilent 6400 Series Triple Quadrupole LC/MS/MS Users Session Agilent 6400 Series Triple Quadrupole LC/MS/MS Users Session QQQ Method Development and Optimization MassHunter Quant: Method setup Peak detection optimization Quant troubleshooting David Presser Application

More information

Analysis of Stachydrine in Leonurus japonicus Using an Agilent ZORBAX RRHD HILIC Plus Column with LC/ELSD and LC/MS/MS

Analysis of Stachydrine in Leonurus japonicus Using an Agilent ZORBAX RRHD HILIC Plus Column with LC/ELSD and LC/MS/MS Analysis of Stachydrine in Leonurus japonicus Using an Agilent ZORBAX RRHD HILIC Plus Column with LC/ELSD and LC/MS/MS Application Note Traditional Chinese Medicine Author Rongjie Fu Agilent Technologies

More information

Improved Automated Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS

Improved Automated Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS Improved Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS Abstract In this work, we demonstrate an improved automated sample preparation for liquid chromatography-tandem mass spectrometry

More information

ABI 3200 Q TRAP LC/MS/MS System

ABI 3200 Q TRAP LC/MS/MS System www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL 001.847.913.0777 ABI 3200 Q TRAP LC/MS/MS System The advantages of an ion trap and the performance of a triple quad. All in one system.

More information

Application Note. Abstract. Authors. Environmental

Application Note. Abstract. Authors. Environmental Determination of Acidic Herbicides using an Agilent 6460 Triple Quadrupole LC/MS Equipped with Agilent Jet Stream Technology and Direct Aqueous Injection, for Potable and Environmental Samples Application

More information

Key Words Q Exactive, Accela, MetQuest, Mass Frontier, Drug Discovery

Key Words Q Exactive, Accela, MetQuest, Mass Frontier, Drug Discovery Metabolite Stability Screening and Hotspot Metabolite Identification by Combining High-Resolution, Accurate-Mass Nonselective and Selective Fragmentation Tim Stratton, Caroline Ding, Yingying Huang, Dan

More information

Protein Sequencer PPSQ-31A / 33A C297-E055B

Protein Sequencer PPSQ-31A / 33A C297-E055B Protein Sequencer PPSQ-31A / 33A C297-E055B Protein Sequencer PPSQ-31A / 33A Achieving Greater Simplicity and Reliability in the Determination of Amino Acid Sequences Analytical Stability Excellent baseline

More information

Rapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS

Rapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS Rapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS Application Note Forensic Toxicology Authors Martin Josefsson, and Markus Roman National Board of Forensic Medicine Linköping,

More information

Structural Analysis by In-Depth Impurity Search Using MetID Solution and High Accuracy MS/MS

Structural Analysis by In-Depth Impurity Search Using MetID Solution and High Accuracy MS/MS C146-E118 Structural Analysis by In-Depth Impurity Search Using MetID Solution and High Accuracy MS/MS Technical Report vol.16 1. Introduction MetID Solution is a software application that was developed

More information

Agilent G3212 GC-APCI Source

Agilent G3212 GC-APCI Source Agilent G3212 GC-APCI Source Quick Start Guide Where to find information 2 Getting Started 3 Step 1. Start the Data Acquisition program for the GC and the Q-TOF 3 Step 2. Prepare the GC and Q-TOF for data

More information

Agilent 6400 Series Triple Quadrupole Users Workshop. MH QQQ Users workshop 2/21/2014 1

Agilent 6400 Series Triple Quadrupole Users Workshop. MH QQQ Users workshop 2/21/2014 1 Agilent 6400 Series Triple Quadrupole Users Workshop 1 Agilent 6400 Series Triple Quadrupole Users Workshop QQQ Method Development and Optimization MassHunter Quantitation: Batch and Method setup QC s,

More information

High-Throughput Protein Quantitation Using Multiple Reaction Monitoring

High-Throughput Protein Quantitation Using Multiple Reaction Monitoring High-Throughput Protein Quantitation Using Multiple Reaction Monitoring Application Note Authors Ning Tang, Christine Miller, Joe Roark, Norton Kitagawa and Keith Waddell Agilent Technologies, Inc. Santa

More information

Chiral Separation Using SFC and HPLC

Chiral Separation Using SFC and HPLC PO-CON6E Chiral Separation Using and HPLC Pittcon 06 760-6 Satoru Watanabe, Hidetoshi Terada, Takato Uchikata, Yohei Arao, Kenichiro Tanaka, Yasuhiro Funada Shimadzu Corporation, Kyoto, Japan; Shimadzu

More information

A Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies

A Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies A Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies APPLICATION BENEFITS Regulatory guidelines and recommendations place a greater emphasis on the detection

More information

Analyst Software. Automatic Optimization Tutorial

Analyst Software. Automatic Optimization Tutorial This document is provided to customers who have purchased AB Sciex equipment to use in the operation of such AB Sciex equipment. This document is copyright protected and any reproduction of this document

More information

MassHunter METLIN Metabolite PCD/PCDL Quick Start Guide

MassHunter METLIN Metabolite PCD/PCDL Quick Start Guide MassHunter METLIN Metabolite PCD/PCDL Quick Start Guide What is the MassHunter METLIN Metabolite PCD/PCDL? 2 Where to find more information 2 Kit Content 3 Installation 5 Before you start 5 Install MassHunter

More information

Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry

Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Application Note Food Authors Jianqiu Mi, Zhengxiang Zhang, Zhixu Zhang,

More information

2010 Waters Corporation. Xevo TQ-S for Bioanalysis

2010 Waters Corporation. Xevo TQ-S for Bioanalysis Xevo TQ-S for Bioanalysis Key innovations High Performance Stepwave RADAR Scan Wave Versatility Ion Sources Simplicity of Operation Engineered Simplicity The Xevo TQ family Built on the same foundations

More information

Development of high speed CYP cocktail inhibition assay using UHPLC-MS/MS

Development of high speed CYP cocktail inhibition assay using UHPLC-MS/MS PO-CON E Development of high speed CYP cocktail inhibition assay using ASMS 213 W2-4 Ichiro Hirano 1, Miho Kawashima 1, Natsuyo Asano 1, Kiyomi Arakawa 1, Yoshihiro Hayakawa 1 1 Shimadzu Corporation. 1,

More information

Quantitation of High Resolution MS Data Using UNIFI: Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays

Quantitation of High Resolution MS Data Using UNIFI: Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays : Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays Mark Wrona, Jayne Kirk, and Yun Alelyunas Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Ability

More information

Quantitative Analysis of Opioids Using a Triple-Quadrupole GC/MS/MS

Quantitative Analysis of Opioids Using a Triple-Quadrupole GC/MS/MS PO-CON1408E Quantitative Analysis of Opioids Using a Pittcon 2014 1090-8P Laura Chambers, Richard Whitney, Ph.D., Nicole M. Lock, Zhuangzhi Max Wang, Ph.D., Clifford M. Taylor; Shimadzu Scientific Instruments,

More information

Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes

Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes Application Note Food Safety Author Sunil Kulkarni Agilent Technologies

More information

Utility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery

Utility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery Application Note: 3 Utility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery Yoko Yamagishi, Thermo Fisher Scientific, C-2F 3-9

More information

Forensic and toxicological drug screening using liquid chromatography-tandem mass spectrometry with MS/MS library based identification

Forensic and toxicological drug screening using liquid chromatography-tandem mass spectrometry with MS/MS library based identification POCON696E Forensic and toxicological drug screening using liquid chromatographytandem mass spectrometry with MS/MS library based identification MSACL 6 EU Alan Barnes, Tiphaine Robin, Neil Loftus, Pierre

More information

A Workflow Approach for the Identification and Structural Elucidation of Impurities of Quetiapine Hemifumarate Drug Substance

A Workflow Approach for the Identification and Structural Elucidation of Impurities of Quetiapine Hemifumarate Drug Substance A Workflow Approach for the Identification and Structural Elucidation of Impurities of Quetiapine Hemifumarate Drug Substance Michael D. Jones, Marian Twohig, Karen Haas, and Robert S. Plumb Waters Corporation,

More information

Sensitive Femtogram Determination of Aflatoxins B 1 , B 2 , G 1. and G 2. in Food Matrices using Triple Quadrupole LC/MS. Authors.

Sensitive Femtogram Determination of Aflatoxins B 1 , B 2 , G 1. and G 2. in Food Matrices using Triple Quadrupole LC/MS. Authors. Sensitive Femtogram Determination of Aflatoxins B 1, B 2, G 1 and G 2 in Food Matrices using Triple Quadrupole LC/MS Application Note Food Safety Authors Yang Chen and Jack Cappozzo National Center for

More information

TOXICOLOGY & FORENSIC APPLICATIONS

TOXICOLOGY & FORENSIC APPLICATIONS TOXICOLOGY & FORENSIC APPLICATIONS New Developments of Confirmation Instruments Applications in the Poison Control Centre's Thermo Scientific Instrumentation & their integrated solution s specialize in

More information

+ butanal + 2-picolineborane. + acetic acid. + butanal + 2-picolineborane. + acetic acid

+ butanal + 2-picolineborane. + acetic acid. + butanal + 2-picolineborane. + acetic acid PO-CON1751E Quantitation of plasma metanephrine and normetanephrine by derivatization using an integrated LC-MS/MS analyzer equipped with fully-automated sample preparation device ASMS 2017 ThP-099 Atsuhiko

More information

Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer

Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer APPLICATION NOTE 72385 Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer Authors Stephan Meding, Katherine Lovejoy, Martin Ruehl Thermo Fisher Scientific,

More information

C HA R AC T E RIZ AT IO N O F INK J E T P RINT E R C A RT RIDG E INK S USING A CHEMOMETRIC APPROACH

C HA R AC T E RIZ AT IO N O F INK J E T P RINT E R C A RT RIDG E INK S USING A CHEMOMETRIC APPROACH C HA R AC T E RIZ AT I N F INK J E T P RINT E R C A RT RIDG E INK S USING A CHEMMETRIC APPRACH Diana Uría and Paul Silcock Waters Corporation, Manchester, UK AIM To evaluate the potential of UPLC -ToF-MS

More information

High Performance Liquid Chromatography. Table 1: Allowed HPLC Adjustment of USP <621> and EP <2.2.46>

High Performance Liquid Chromatography. Table 1: Allowed HPLC Adjustment of USP <621> and EP <2.2.46> High Performance Liquid Chromatography HPLC-9 Ultra-high Speed Analysis of Ibuprofen within USP Allowed Limits by Nexera Method Scouting In recent years, high-throughput analytical techniques have

More information

Accurate Mass Analysis of Hydraulic Fracturing Waters

Accurate Mass Analysis of Hydraulic Fracturing Waters Application Note Environmental Accurate Mass Analysis of Hydraulic Fracturing Waters Using the Kendrick mass defect with the Agilent LC/Q-TOF MS Authors E. Michael Thurman and Imma Ferrer Department of

More information

Proudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment

Proudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment Applied Biosystems QStar Pulsar i Features of the API QSTAR Pulsar i The

More information

Evaluation of a novel glass vial overcoming adsorption effect for pharmaceutical drugs

Evaluation of a novel glass vial overcoming adsorption effect for pharmaceutical drugs PO-CON1577E Evaluation of a novel glass vial overcoming adsorption effect for pharmaceutical drugs HPLC 215 PSB-PHARM-17 Minori Nakashima*(1), Yuki Sato (2,3), Takeshi Kuwahara (3), Azusa Uchida (1), Takenao

More information

Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer

Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer 38 Current Trends in Mass Spectrometry November 6 Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer This article demonstrates the improved

More information

WADA Technical Document TD2003IDCR

WADA Technical Document TD2003IDCR IDENTIFICATION CRITERIA FOR QUALITATIVE ASSAYS INCORPORATING CHROMATOGRAPHY AND MASS SPECTROMETRY The appropriate analytical characteristics must be documented for a particular assay. The Laboratory must

More information

Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research

Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Heather A Brown, 1 Claudia Rossi, 2 and Lisa J Calton 1 1 Waters Corporation, Wilmslow, UK 2

More information

LC-MS/MS Method for the Determination of Diclofenac in Human Plasma

LC-MS/MS Method for the Determination of Diclofenac in Human Plasma LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core

More information

Liquid Chromatography Mass Spectrometry. Shimadzu Corporation, UK; 2 Phytocontrol, France; 3 Shimadzu France, France. Trimesium S + CH 3 ETU

Liquid Chromatography Mass Spectrometry. Shimadzu Corporation, UK; 2 Phytocontrol, France; 3 Shimadzu France, France. Trimesium S + CH 3 ETU LAA-A-LM-E89 Liquid Chromatography Mass Spectrometry LCMS C8 Highly Polar Pesticide Multi-Residue Analysis in Food Safety by LC-MS/MS David R. Baker, Eric Capodanno, Mikaël Levi Shimadzu Corporation, UK;

More information

Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS

Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 129 Infi nity LC, and 646 Triple Quadrupole LC/MS Application Note Clinical Research Authors Linda Côté and Christophe

More information

Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System

Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System Application Note Authors Mohamed Youssef and Vaughn P. Miller Agilent

More information

Q-TOF PREMIER DYNAMIC RANGE ENHANCEMENT AND ACCURATE MASS MEASUREMENT PERFORMANCE

Q-TOF PREMIER DYNAMIC RANGE ENHANCEMENT AND ACCURATE MASS MEASUREMENT PERFORMANCE Q-TOF PREMIER DYAMIC RAGE EHACEMET AD ACCURATE MASS MEASUREMET PERFORMACE Michael McCullagh, Jose Castro-Perez, and Lisa Calton Waters Corporation, Manchester, UK ITRODUCTIO The use of time-of-flight (TOF)

More information

MassHunter TOF/QTOF Users Meeting

MassHunter TOF/QTOF Users Meeting MassHunter TOF/QTOF Users Meeting 1 Qualitative Analysis Workflows Workflows in Qualitative Analysis allow the user to only see and work with the areas and dialog boxes they need for their specific tasks

More information

Bioanalytical Chem: 4590: LC-MSMS of analgesics LC-MS Experiment Liquid Chromatography Mass Spectrometry (LC/MS)

Bioanalytical Chem: 4590: LC-MSMS of analgesics LC-MS Experiment Liquid Chromatography Mass Spectrometry (LC/MS) Liquid Chromatography Mass Spectrometry (LC/MS) Prelab Questions: Questions to be answered before doing the experiment. The answers are due at the beginning of each experiment without exception (the questions

More information

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS Christopher Borton AB SCIEX Golden, Colorado verview Described here is the analysis of

More information

Agilent 6495 Triple Quadrupole LC/MS System EXPERIENCE A NEW LEVEL OF CONFIDENCE

Agilent 6495 Triple Quadrupole LC/MS System EXPERIENCE A NEW LEVEL OF CONFIDENCE Agilent 49 Triple Quadrupole LC/MS System EXPERIENCE A NEW LEVEL OF CONFIDENCE AGILENT 49 TRIPLE QUADRUPOLE LC/MS SYSTEM WORK SMARTER, DO MORE ifunnel new Q1 Optics new Ion Detector IMPROVED SENSITIVITY

More information

Proudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment LCQ Deca XP Plus

Proudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment LCQ Deca XP Plus www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment LCQ Deca XP Plus Improved Ion Optics for Greater Sensitivity and Precision

More information

Chemistry Instrumental Analysis Lecture 37. Chem 4631

Chemistry Instrumental Analysis Lecture 37. Chem 4631 Chemistry 4631 Instrumental Analysis Lecture 37 Most analytes separated by HPLC are thermally stable and non-volatile (liquids) (unlike in GC) so not ionized easily by EI or CI techniques. MS must be at

More information

Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS. Application. Authors. Abstract.

Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS. Application. Authors. Abstract. Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS Application Food Analysis Authors Sheher Mohsin Agilent Technologies, Inc. Schaumburg, Il USA Michael Zumwalt

More information

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS

EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS Application Note EPA Method 535 EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS API 3200 LC/MS/MS System Overview Described here is the

More information

Mass Spectrometry in MCAL

Mass Spectrometry in MCAL Mass Spectrometry in MCAL Two systems: GC-MS, LC-MS GC seperates small, volatile, non-polar material MS is detection devise (Agilent 320-MS TQ Mass Spectrometer) Full scan monitoring SIM single ion monitoring

More information

Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative. Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish.

Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative. Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish. 1 Supporting Information 2 3 Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish. 4 5 John Moreman, Okhyun

More information

MassHunter Software Overview

MassHunter Software Overview MassHunter Software Overview 1 Qualitative Analysis Workflows Workflows in Qualitative Analysis allow the user to only see and work with the areas and dialog boxes they need for their specific tasks A

More information

Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma

Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Jon Bardsley 1, James Goldberg 2 1 Thermo Fisher Scientific, Runcorn, UK; 2 Thermo Fisher Scientific, West Palm Beach,

More information

Agilent All Ions MS/MS

Agilent All Ions MS/MS Agilent All Ions MS/MS Workflow Overview A Determine fragment ions for LC/MS Quant method B Develop final Quant method Develop LC/MS Qualitative Analysis method Process data with Find by Formula Build

More information

Spectrophotometer for Life Science. BioSpec-nano C101-E112D

Spectrophotometer for Life Science. BioSpec-nano C101-E112D Spectrophotometer for Life Science BioSpec-nano C11-E112D BioSpec-nano Spectrophotometer for Life Science Drop-and-Start Analysis Automatic Optical Pathlength Setting & Automatic Wiping Low Carryover Achieved

More information