Potential of metatranscriptomics as indicator for ecosystem level diversity and function. diversitv Prof. Dr. Jens Boenigk. Department.

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1 Department Bio diversitv Prof. Dr. Jens Boenigk

2 From traditional bioindication......via metabarcoding & molecular diversity......to monitoring functional genes

3 From traditional bioindication......via metabarcoding & molecular diversity......to monitoring functional genes

4 From traditional bioindication......via metabarcoding & molecular diversity......to monitoring functional genes

5 Amplicon diversity more than just barcoding indicator species

6 Amplicon diversity more than just barcoding indicator species Grossmann et al. (2016) ISME Journal

7 Amplicon diversity more than just barcoding indicator species Error reduction: Separating true biological diversity from sequence artifatcs Grossmann et al. (2016) ISME Journal

8 Amplicon diversity more than just barcoding indicator species Lange et al. (2016) PLOS One

9 No of OTUs Amplicon diversity more than just barcoding indicator species Read abundance Lange et al. (2016) PLOS One

10 No of OTUs Amplicon diversity more than just barcoding indicator species Read abundance Without filter Lange et al. (2016) PLOS One

11 No of OTUs Amplicon diversity more than just barcoding indicator species Read abundance Without filter AmpliconDuo Sample split protocols allow for separating true biological diversity from artifacts Still limited to indirect approaches to ecosystem function and quality Lange et al. (2016) PLOS One

12 Metatranscriptomis & Metagenomics: Bioindication by using the full potential of molecular diversity

13 Relative difference Grossmann et al. (2016) Mol Ecol

14 Relative difference Grossmann et al. (2016) Mol Ecol

15 Relative difference Grossmann et al. (2016) Mol Ecol

16 High level of functional redundancy Relative difference Grossmann et al. (2016) Mol Ecol

17 High level of functional redundancy Relative difference What are the potential benefits of functional diversity in biomonitoring? Grossmann et al. (2016) Mol Ecol

18 Nuy et al. submitted

19 P-limiting conditions are indicated by an Up-regulation of transporter genes Metatranscriptomic & Metagenommic data allow for addressing environmental status in more detail Nuy et al. submitted

20 Concerted analyses of - amplicon diversity - transcriptome / genome analyses of key taxa - metatranscriptomic / metagenomic field surveys have the potential to indicate ecosystem properties in more detail and precisely Fish Metazooplankton Production Cyanobacteria Heterotrophic protists Phototrophic protists Viruses Het. bacteria Jens Boenigk, aus Boenigk J, Wodniok S (2014) Biodiversität und Erdgeschichte. Springer, Berlin Heidelberg. Dissolved organic carbon (DOC)

21 Department Biodiversity, Faculty of Biology, University Duisburg-Essen Daniela Beisser Genome Bioinformatics Christina Bock Phylogeny, Systematics, Ecophysiology Sabina Wodniok Phylogenomics, Transcriptome analysis Open PhD positions: Post-glacial distribution pattern & biogeography of protists in Europe (University Oldenburg) Taxonomic & molecular diversity of golden algae (Chrysophyceae) (University Duisburg-Essen) Manfred Jensen Physiology, Statistics Lars Großmann, Nadine Nerat, Julia Nuy, Stephan Majda; many Bachelor & Master students Department Bioinformatics (Daniel Hoffmann) Department Genome Bioinformatics (Sven Rahmann) Department Aquatic Ecology (Bernd Sures, Daniel Hering)