Introduction to Bioinformatics

Size: px

Start display at page:

Download "Introduction to Bioinformatics"

Arron Russell
5 years ago
Views:

1 Introduction to Bioinformatics Lecture : p he biological problem p lobal alignment p Local alignment p Multiple alignment 6 Background: comparative genomics p Basic question in biology: what properties are shared among organisms? p enome sequencing allows comparison of organisms at DN and protein levels p Comparisons can be used to 64 Find evolutionary relationships between organisms Identify functionally conserved sequences Identify corresponding genes in human and model organisms: develop models for human diseases omologs 65 p wo genes (sequences in general) and evolved from the same ancestor gene g are called homologs p omologs usually exhibit conserved functions p Close evolutionary relationship => expect a high number of homologs g = agtgtccgttaagtgcgttc = agtgccgttaaagttgtacgtc = ctgactgtttgtggttc Sequence similarity p e expect homologs to be similar to each other p Intuitively, similarity of two sequences refers to the degree of match between corresponding positions in sequence p hat about sequences that differ in length? 66 agtgccgttaaagttgtacgtc ctgactgtttgtggttc Similarity vs homology p Sequence similarity is not sequence homology If the two sequences and have accumulated enough mutations, the similarity between them is likely to be low #mutations agtgtccgttaagtgcgttc agtgtccgttatagtgcgttc agtgtccgcttatagtgcgttc 4 agtgtccgcttaagggcgttc 8 agtgtccgcttcaaggggcgt 6 gggccgttcatgggggt gcagggcgtcactgagggct 67 #mutations 64 acagtccgttcgggctattg 8 cagagcactaccgc 56 cacgagtaagatatagct 5 taatcgtgata 4 acccttatctacttcctggagtt 48 agcgacctgcccaa 496 caaac omology is more difficult to detect over greater evolutionary distances.

Similarity vs homology () p Sequence similarity can occur by chance Similarity does not imply homology p Consider comparing two short sequences against each other Orthologs and paralogs p e

ene duplication event g Organism g g Organism B Orthologs OrganismC Paralogs 68 69 Orthologs and paralogs () p Orthologs typically retain the original function p In paralogs, one copy is free to

2 Similarity vs homology () p Sequence similarity can occur by chance Similarity does not imply homology p Consider comparing two short sequences against each other Orthologs and paralogs p e distinguish between two types of homology Orthologs: homologs from two different species, separated by a speciation event Paralogs: homologs within a species, separated by a gene duplication event g ene duplication event g Organism g g Organism B Orthologs OrganismC Paralogs Orthologs and paralogs () p Orthologs typically retain the original function p In paralogs, one copy is free to mutate and acquire new function (no selective pressure) g g g g Organism Paralogy example: hemoglobin p emoglobin is a protein complex which transports oxygen p In humans, hemoglobin consists of four protein subunits and four nonprotein heme groups Organism B OrganismC Sickle cell diseases are caused by mutations in hemoglobin genes emoglobin, Paralogy example: hemoglobin p In adults, three types are normally present emoglobin : alpha and beta subunits emoglobin : alpha and delta subunits emoglobin F: alpha and gamma subunits p Each type of subunit (alpha, beta, gamma, delta) is encoded by a separate gene emoglobin, Paralogy example: hemoglobin p he subunit genes are paralogs of each other, i.e., they have a common ancestor gene p Exercise: hemoglobin human paralogs in NCBI sequence databases z?db=nucleotide Find human hemoglobin alpha, beta, gamma and delta Compare sequences emoglobin, 7 7

3 Orthology example: insulin p he genes coding for insulin in human (omo sapiens) and mouse (Mus musculus) are orthologs: hey have a common ancestor gene in the ancestor species of human and mouse Exercise: find insulin orthologs from human and mouse in NCBI sequence databases p lignment specifies which positions in two sequences match actctag matches 5 mismatches not aligned actctag 5 matches mismatches not aligned actctag 7 matches mismatches not aligned Mutations: Insertions, deletions and substitutions p Maximum alignment length is the total length of the two sequences Indel: insertion or deletion of a base with respect to the ancestor sequence actctag Mismatch: substitution (point mutation) of a single base actctag matches mismatches 5 not aligned actctag matches mismatches 5 not aligned p Insertions and/or deletions are called indels e can t tell whether the ancestor sequence had a base or not at indel position! Problems Sequence lignment (chapter 6) p hat sorts of alignments should be considered? p ow to score alignments? p ow to find optimal or good scoring alignments? p ow to evaluate the statistical significance of scores? p he biological problem p lobal alignment p Local alignment p Multiple alignment In this course, we discuss each of these problems briefly

4 lobal alignment p Problem: find optimal scoring alignment between two sequences (Needleman & unsch 97) p Every position in both sequences is included in the alignment p e give score for each position in alignment Identity (match) + Substitution (mismatch) µ Indel p otal score: sum of position scores Scoring: oy example p Consider two sequences with characters drawn from the English language alphabet:, S(/) = + µ S(/) = µ µ µ 8 8 Dynamic programming p ow to find the optimal alignment? p e use previous solutions for optimal alignments of smaller subsequences p his general approach is known as dynamic programming Introduction to dynamic programming: the money change problem p Suppose you buy a pen for 4. and pay for with a 5 note p ou get 77 cents in change what coins is the cashier going to give you if he or she tries to minimise the number of coins? p he usual algorithm: start with largest coin (denominator), proceed to smaller coins until no change is left: 5,, 5 and cents p his greedy algorithm is incorrect, in the sense that it does not always give you the correct answer 8 8 he money change problem he money change problem p ow else to compute the change? p e could consider all possible ways to reduce the amount of change p Suppose we have 77 cents change, and the following coins: 5,, 5 cents p e can compute the change with recursion C(n) = min { C(n 5) +, C(n ) +, C(n 5) + } p Figure shows the recursion tree for the example p Many values are computed more than once! p his leads to a correct but very inefficient algorithm p e can speed the computation up by solving the change problem for all i n Example: solve the problem for 9 cents with available coins being, and 5 cents p Solve the problem in steps, first for cent, then cents, and so on p In each step, utilise the solutions from the previous steps

5 he money change problem mount of change left Number of coins used p lgorithm runs in time proportional to Md, where M is the amount of change and d is the number of coin types p he same technique of storing solutions of subproblems can be utilised in aligning sequences Representing alignments and scores lignments can be represented in the following tabular form. Each alignment corresponds to a path through the table Representing alignments and scores Representing alignments and scores lobal alignment score S,4 = µ µ Filling the alignment matrix Filling the alignment matrix () Case Case Case Consider the alignment process at shaded square. Case. lign against (match) Case. lign in against (indel) in Case. lign in against (indel) in Case Case Case Scoring the alternatives. Case. S, = S, + s(, ) Case. S, = S, Case. S, = S, s(i, j) = for matching positions, s(i, j) = µ for substitutions. Choose the case (path) that yields the maximum score. Keep track of path choices

6 lobal alignment: formal development Scoring partial alignments = a a a a n, B = b b b b m b b b b 4 a a a ny alignment can be written as a unique path through the matrix Score for aligning and B up to positions i and j: S i,j = S(a a a a i, b b b b j ) a a a b b b 4 b 4 p lignment of = a a a a i with B = b b b b j can be end in three possible ways Case : (a a a i ) a i (b b b j ) b j Case : (a a a i ) a i (b b b j ) Case : (a a a i ) (b b b j ) b j 9 9 Scoring alignments Scoring alignments () p Scores for each case: Case : (a a a i ) a i (b b b j ) b j Case : (a a a i ) a i (b b b j ) Case : (a a a i ) (b b b j ) b j + ifa i = b j s(a i, b j ) = { µ otherwise s(a i, ) = s(, b j ) = p First row and first column correspond to initial alignment against indels: S(i, ) = i S(, j) = j p Optimal global alignment score S(, B) = S n,m a a b b b 4 b 4 4 a lgorithm for global alignment lobal alignment: example Input sequences, B, n =, m = B Set S i, := i for all i Set S,j := j for all j for i := to n for j := to m S i,j := max{s i,j, S i,j + s(a i,b j ), S i,j } end end µ = = C ? lgorithm takes O(nm) time

7 lobal alignment: example lobal alignment: example () µ = µ = = = C 6 8 C C ?

Background: comparative genomics. Sequence similarity. Homologs. Similarity vs homology (2) Similarity vs homology. Sequence Alignment (chapter 6)

Sequence lignment (chapter ) he biological problem lobal alignment Local alignment Multiple alignment Background: comparative genomics Basic question in biology: what properties are shared among organisms?