Browsing Genes and Genomes with Ensembl
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1 Training materials Ensembl training materials are protected by a CC BY license If you wish to re-use these materials, please credit Ensembl for their creation If you use Ensembl for your work, please cite our papers
2 Browsing Genes and Genomes with Ensembl Ben Moore Ensembl Outreach Officer EMBL-EBI
3 Objectives What is Ensembl? What type of data can you get in Ensembl? How to navigate the Ensembl browser website. How to use Ensembl tools Where to go for help and documentation.
4 This webinar course All webinars begin at 9am BST Date Webinar topic Instructor 6th April Introduction to Ensembl Helen Sparrow 13th April Ensembl genes Emily Perry 20th April Data export with BioMart Victoria Newman 27th April Variation data in Ensembl and the Ensembl VEP Victoria Newman 4th May Comparing genes and genomes with Ensembl Compara Ben Moore 11th May Finding features that regulate genes the Ensembl Regulatory Build Ben Moore 18th May Uploading your data to Ensembl and advanced ways to access Ensembl data Emily Perry
5 Structure Presentation: What the data/tool is How we produce/process the data Demo: Getting the data Using the tool Exercises: On the train online course
6 Questions? Ask questions in the Chat box in the webinar interface My Ensembl colleagues will respond during There s no threading so please respond Emily Perry Victoria Newman
7 Course exercises ebinar-series-2016 A link to exercises and their solutions are in the page hierarchy This text will be replaced by a YouTube (link to YouKu too) video of this webinar and a pdf of the slides The next page is the exercises for this module
8 Get help with the exercises Use the exercise solutions in the online course Join our Facebook group and discuss the exercises with everybody (see the online course for the link) us:
9 Comparative Genomics EBI is an Outstation of the European Molecular Biology Laboratory.
10 Overview of the talk - Comparative genomics: applications and species Gene trees Homology predictions Whole genome alignments - pairwise - multiple - Shared synteny
11 Applications of Comparative Genomics Comparative genomics allows us to understand: - vertebrate evolution - differences between species at the genome level - gene function based on homology - the distribution of highly conserved regions
12 Comparative analysis by taxa Ensembl Compara Ensembl Metazoa Compara
13 Pan-taxonomic compara genomes?pan_compara=1
14 Pan-taxonomic compara Ensembl Metazoa Compara
15 Gene trees - Based on protein alignments - Representative protein of each Ensembl gene - Clustering, Blast, multiple alignments - Reconciliation with species tree - Orthologue/Paralogue inference Orthologues and Paralogues ra/homology_method.html
16 Homology relationships Paralogues Orthologues Genes emerged through a duplication event eg c1 and c2 h1 and h2 Genes emerged through a speciation event eg c1 and h1 h2 and m c2 and m c1 h1 One-to-one c2 h2 m One-to-many Speciation Duplication
17 Hands on - We re going to look at a gene BRCA2 to find homologues.
18 Whole genome alignments - To identify highly conserved regions - sequences that evolve slowly - regions likely to be functional - both coding and non-coding sequences - To spot trouble gene predictions - To define syntenic regions - Types: pairwise versus multiple
19 Alignments - Pairwise alignments with BLASTZ (older) LASTZ-net (newer) - EPO (Enredo-Pecan-Ortheus) analysis - - For selected sets (11 fish, 7 sauropsids, 40 eutherian, 8 primates) Mercator-Pecan analysis - For 24 amniota vertebrates (mammals+birds)
20 Shared synteny
21 Hands on - We will look at a human genomic region 2: which contains the HoxD cluster to find alignments and conservation regions.
22 This webinar course Date Webinar topic Instructor 6th April Introduction to Ensembl Helen Sparrow 13th April Ensembl genes Emily Perry 20th April Data export with BioMart Victoria Newman 27th April Variation data in Ensembl and the Ensembl VEP Victoria Newman 4th May Comparing genes and genomes with Ensembl Compara Ben Moore 11th May Finding features that regulate genes the Ensembl Regulatory Build Ben Moore 18th May Uploading your data to Ensembl and advanced ways to access Ensembl data Emily Perry
23 Course exercises ser-webinar-series-2016 This text will be replaced by a YouTube (link to YouKu too) video of the webinar and a pdf of the slides. A link to exercises and their solutions will appear in the page hierarchy The next page will be the exercises
24 Get help with the exercises Use the exercise solutions in the online course Join our Facebook group and discuss the exercises with everybody (see the online course for the link) us:
25 Help and documentation Course online Tutorials Flash animations us Ensembl public mailing lists
26 Follow us
27 Publications Aken, BL. et al Ensembl 2017 Nucleic Acids Research Xosé M. Fernández-Suárez and Michael K. Schuster Using the Ensembl Genome Server to Browse Genomic Sequence Data. Current Protocols in Bioinformatics (2010) Giulietta M Spudich and Xosé M Fernández-Suárez Touring Ensembl: A practical guide to genome browsing BMC Genomics 11:295 (2010) Javier Herrero et al Ensembl Comparative Genomics Resources Database (Oxford) 2016: bav096
28 Ensembl 2017
29 Ensembl Acknowledgements
30 Training materials Ensembl training materials are protected by a CC BY license If you wish to re-use these materials, please credit Ensembl for their creation If you use Ensembl for your work, please cite our papers
Comparative Genomics. EBI is an Outstation of the European Molecular Biology Laboratory.
Training materials Ensembl training materials are protected by a CC BY license http://creativecommons.org/licenses/by/4.0/ If you wish to re-use these materials, please credit Ensembl for their creation
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