Life of the Cell. Learning Objectives

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1 Life of the Cell Society on a micro-scale 1 Learning Objectives 1. What are the characteristics that distinguish prokaryotic and eukaryotic cells? Which type of cell is believed to be older (more primitive)? 2. Describe the four characteristics shared by all cells. 3. Describe the structure and function of the various eukaryotic cell organelles. What adaptive advantage(s) is/are associated with having organelles? 4. Describe the theories that describe the origin of membranous organelles in eukaryotic cells. 1

2 Cellular Diversity in the Human Body A huge population of trillion cells in the adult 200 different types of cells What does cell shape tell you about its function? 3 The Typical Animal Cell Not all cells contain all of these organelles. 4 2

3 Identify the 3 types of lipid molecules found in cell membranes: glycolipids, cholesterol and phospholipids 5 Membrane Proteins Integral versus Peripheral Proteins 6 3

4 Functions of Membrane Proteins Formation of Channel passageway to allow specific substance to pass through Transporter Proteins bind a specific substance, change their shape & move it across membrane Receptor Proteins cellular recognition site -- bind to substance 7 Functions of Membrane Proteins Cell Identity Marker allow cell to recognize other similar cells Cell Adhesion (Linker) anchor proteins in cell membrane or to other cells allow cell movement cell shape & structure Act as Enzyme speed up reactions 8 4

5 The Transmembrane Electrochemical Potential Concentration gradient What other solutes are distributed differently across the cell membrane? (review the slide from the intro. presentation) Electrical gradient Which solute(s) contribute to the net negative charge inside cells? 9 Transport Across the Plasma Membrane Differentiate between active and passive transport. Do all transport proteins require ATP to move substances? Why do substances cross membranes passively, i.e. what is the force that drives passive transport? 10 5

6 Diffusion Crystal of dye placed in a cylinder of water Describe the net direction of the movement of dye molecules. Explain Describe the movement of dye molecules in the third cylinder 11 Factors That Affect the Rate of Diffusion Across Cell Membranes Describe the properties of materials that will diffuse through a cell membrane. What kinds of materials do not diffuse through cell membranes? 12 6

7 Osmosis of Water Through a Membrane Pure water in the left arm & a membrane impermeable to the solute found in the right arm Net movement of water is down the concentration gradient for water, until equilibrium is reached Osmotic pressure is equal to the hydrostatic pressure necessary to stop the net movement of water molecules 13 Effects of Tonicity on Cell Membranes Isotonic solution water concentration the same inside & outside of cell results in no net movement of water across cell membrane Hypotonic solution higher concentration of water outside of cell results in hemolysis Hypertonic solution lower concentration of water outside of cell causes crenation 14 7

8 Diffusion Through Membrane Channels Each membrane channel is specific for particular ion (K +, Cl-, Na + or Ca +2 ) Slower than diffusion through membrane, but still 1million K + through a channel in one second Channels may be open all the time, or gated (closed randomly or as directed ) How might channels be regulated? Name one stimulus to open. 15 Facilitated Diffusion of Glucose Glucose binds to transport protein Transport protein changes shape Glucose moves across cell membrane (but only down the concentration gradient) Kinase enzyme reduces glucose concentration inside the cell by transforming glucose into glucose-6- phosphate Are these GluT transporters always present in cell membranes? 16 8

9 Glut 4 Transporter The receptor for the hormone insulin is a complex protein that acts as a catalyst, causing fusion of Glut 4 transporter vesicles with the surface membrane (a process similar to exocytosis). 17 Primary Active Transport Transporter protein called a pump works against concentration gradient requires 40% of cellular ATP Na + /K + ATPase pump most common example all cells have 1000s of them maintains low concentration of Na + and a high concentration of K + in the cytosol operates continually Maintenance of osmotic pressure across membrane cells do not normally shrink or swell due to osmosis & osmotic pressure sodium continually pumped out as if sodium could not enter the cell (factor in osmotic pressure of extracellular fluid) K + inside the cell contributes to osmotic pressure of cytosol What other solutes contribute to osmotic pressure inside cells? 18 9

10 Na+/K+ Pump & ATP As Its Energy Source 1. Na+ binding 2. ATP split 3. Na+pushed out 4. K+ binding 5. Phosphate release 6. K+ is pushed in 3 Na+ ions removed from cell as 2 K+ brought into cell. 19 Antiporters and Symporters (one in & one out) (both going in) 20 10

11 Pinocytosis and Phagocytosis No pseudopods form Nonselective drinking of extracellular fluid Pseudopods extend to form phagosome Lysosome joins it for intracellular digestion 21 Cell Organelles Nonmembranous organelles lack membranes & are indirect contact with cytoplasm Membranous organelles surrounded by one or two lipid bilayer membranes 22 11

12 Cytoskeleton Network of protein filaments throughout the cytosol Functions: cell support and shape organization of chemical reactions cell & organelle movement Examples? Continually reorganized 23 The Cytoskeletonal Filaments Microfilaments thinnest filaments (actin) locomotion & division support microvilli Intermediate filaments several different proteins anchor organelles Microtubules large cylindrical structures (composed of tubulin) flagella, cilia and centrosomes 24 12

13 Ribosomal RNA Large + small subunits made in the nucleolus assembled in the cytoplasm small subunits hold mrna during protein synthesis 25 Smooth & Rough Endoplasmic Reticulum Rough ER is covered with fixed ribosomes. What are the possible destinations for proteins made here? Smooth ER contains enzymes, and may store some cell chemicals

14 Packaging by Golgi Complex Proteins pass from rough ER to golgi complex in transport vesicles Finished proteins exit golgi complex as component of: secretory (for exocytosis), membrane, or storage (lysosome) vesicles Proteins in vesicle membranes fuse with plasma membrane (example?) 27 Lysosomes Membranous vesicles formed in Golgi complex filled with digestive enzymes pumps in H+ ions until internal ph reaches 5.0 Functions digest foreign substances autophagy recycles own organelles autolysis lysosomal damage after death 28 14

15 Mitochondria Double membrane organelle central cavity filled with matrix inner membrane folds known as crista large surface area for chemical reactions of cellular respiration Function generation of ATP powerhouse of cell Mitochondria self-replicate increases with need for ATP circular DNA with 37 genes only inherited from mother 29 Nucleus Large organelle with double membrane nuclear envelope outer membrane continuous with rough ER perforated by cytosol-filled nuclear pores (10X channel pore size) Nucleolus spherical, dark bodies within the nucleus (no membrane) site of ribosomal RNA synthesis 30 15

16 Organization of DNA in Chromosomes 31 Protein Synthesis Instructions for making specific proteins is found in the DNA (your genes) transcribe that information onto a messenger RNA molecule each sequence of 3 nucleotides in DNA is called base triplet each base triplet is transcribed as 3 RNA nucleotides (codon) translate the message into a sequence of amino acids in order to build a protein molecule each codon must be matched by an anticodon found on the trna carrying a specific amino acid 32 16

17 1. Transcription takes place in the nucleus. escribe the roles of he 3 types of RNA nvolved in protein ynthesis. 2. Translation take place at ribosomes, either fixed or free. 33 Normal Cell Division Mitosis (somatic cell division) one parent cell gives rise to 2 identical daughter cells mitosis is nuclear division cytokinesis is cytoplasmic division occurs in billions of cells each day needed for tissue repair and growth Meiosis (reproductive cell division) egg and sperm cell production in testes and ovary only 34 17

18 The Cell Cycle in Somatic Cells Process where cell duplicates its contents & divides in two 23 homologous pairs of chromosomes must be duplicated genes must be passed on correctly to the next generation of cells Mitosis = Nuclear division continuous process divided into 4 stages prophase, metaphase, anaphase & telophase Cytokinesis = division of the cytoplasm 35 Interphase Stage of Cell Cycle Doubling of DNA and centrosome Phases of interphase stage -- G1, S, and G2 G1 = cytoplasmic increase (G0 if never divides again) S = replication of chromosomes G2 = cytoplasmic growth 36 18

19 Replication of Chromosomes Doubling of genetic material during interphase. (S phase) DNA molecules unzip Mirror copy is formed along each old strand. Nitrogenous bases pick up complementary base 2 complete identical DNA molecules formed 37 Stages of Nuclear Division:Mitosis Prophase Metaphase Anaphase Telophase 38 19

20 Control of Cell Destiny Cell destiny is either to remain alive & functioning, to grow & divide or to die Homeostasis must maintain balance between cell multiplication & cell death The protein cyclin builds up during interphase and triggers mitosis Programmed cell death (apoptosis) occurs if a triggering agent turns on suicide enzymes that kills the cell Necrosis is cell death caused by injury or infection 39 20

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