Cell Differentiation and Meiosis

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1 Cell Differentiation and Meiosis Study guide Compare the processes and products of meiosis I and meiosis II. Compare the overall processes and products of meiosis and mitosis. Explain how non disjunction can result in Down Syndrome Explain what makes the many types of adult human cells different. Explain how every cell has the potential to act like every other cell. Explain the applications of stem cell research Compare the properties of embryonic and adult stem cells. Explain the advantages and disadvantages of using adult stem cells to produce replacement tissues. Explain the advantages and disadvantages of ips cells Human Life Cycle: mitosis for development and meiosis for reproduction 1

2 A pancreas cell, eye cell and nerve cell in your body: Are these cells duplicates of the original cell (zygote)? Are these cells genetically identically to each other (same number and type of chromosomes)? Does a eye cell have the gene to make insulin? Why are they different in form and function? Most cells in an adult body are differentiated Differentiated cells are specialized in form and function Differentiated cells have a particular pattern of genes that are expressed and not expressed How do cells become differentiated? 2

3 Cell differentiation occurs during the embryo development Early cells are not differentiated (Stem Cells) during development cells become more specialized (differentiated) by getting some genes activated and some others inactivated NOTE: Not all stem cells have the same level of stemness Stem cells are found in the embryo and adult but vary in their stemness 3

4 Why is studying stem cells useful? Stem Cell Therapy: embryonic stem cells vs. adults stem cells How does their potential as therapy vary? What are the ethical considerations? Special considerations of both? 4

5 ips cells: Induced Pluripotent Stem cells ips cells are much less expensive to create than ES cells generated through therapeutic cloning However, because the "reprogramming" process introduces genetic modifications, the safety of using ips cells in patients is uncertain. Human Life Cycle: mitosis for development and meiosis for reproduction 5

6 Our somatic cells have a total of chromosomes in each cell These cells have two chromosomes of each type Two chromosomes that carry the same genes are called homologous chromosomes In a homologous pair : One of the chromosomes was inherited from father One of the chromosomes was inherited from mother In a homologous pair: both chromosomes have the same genes, but are not necessarily identical (they might be different versions, different sequence of bases) 6

7 Pretend Cell How many chromosomes does this cell have? How many types of chromosomes does this cell have? (note size of chromosomes) Does this cell have homologous pairs of chromosomes? How many pairs? Has this cell been through the S phase? Diploid cells and Haploid cells Diploid cell: symbol (2n) has two sets of chromosomes has PAIRS of HOMOLOGOUS chromosomes all somatic cells are diploid HAPLOID cells: symbol (n) has one set of chromosomes has only one of each kind Gametes are haploid 7

8 Is this cell haploid or diploid? What about this cell? Chromosome Homologous pair of chromosomes 8

9 Gametes are produced by a different type of cell division called meiosis # of cells produced # of chromosomes in daughter cells vs. parent cell One (diploid) mother cell 4 (haploid) daughter cells Table comparing the stages of meiosis STAGES OF MEIOSIS HOW ARE THE CHROMOSOMES ARRANGED DURING METAPHASE? WHAT SEPARATES DURING ANAPHASE? ARE CELLs HAPLOID OR DIPLOID ATH THE END OF THIS STAGE? Meiosis I Meiosis II 9

10 Meiosis 1: homologous chromosomes separate Prophase 1: homologous chromosomes pair up (crossing over can occur) Metaphase 1: homologous pairs line up in the middle (randomly) note: double file Anaphase 1: homologous chromosomes separate Result of meiosis 1: two haploid cells, each chromosome has two chromatids Meiosis II: sister chromatids separate Metaphase 2: chromosomes line up in the middle Anaphase 2: Chromatids separate (note: single file) Result of Meiosis II: four haploid daughter cells 10

11 Accidents during meiosis can alter chromosome number in gametes Cause: Non disjunction the chromosomes in a pair of chromosomes or sister chromatids fail to move apart properly Result: gametes have an extra or a one less chromosome An extra copy of chromosome 21 causes Down Syndrome 11

12 Mitosis vs Meiosis Mitosis Meiosis Number of DNA replication events Number of cytokinesis events Number of daughter cell produced Number of sets of chromosomes in daughter cells Genetic relationship of daughter cells to the parent cell (identical or non identical) Purpose in human body 12

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