ProteomTech, Inc. ProteomTech Pt-Fold Technology. Types of Proteins Refolded. (more than 200 proteins successfully refolded)
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1 ProteomTech, Inc Horton Street Suite 405 Emeryville, California Phone: Fax: ProteomTech Pt-Fold Technology Types of Proteins Refolded (more than 200 proteins successfully refolded) Cell Receptors Proteinases Proteinase Inhibitors Signal Transduction Related Kinases Phosphatases Extracellular Matrix Transcription Factors Oncogenes Cytokines Splicing Factors Adaptor Proteins Angiogenesis Related G protein Regulational Proteins G Protein Family Cellular Enzymes Calcium Binding Proteins Mitochondrial Proteins Hypothetical Proteins CD Protein Extracellular Domain
2 Expression, refolding, and purification of proteins from E. coli Name Form Organism Ref. 1. Pepsinogen Full-length Porcine (1) 2. Pepsinogen N and C domain Porcine (2, 3) 3. Rhizopus-PP Full-length Fungal (4, 5) 4. Thermopsin Fusion Archae (6) 5. HIV protease Full-length HIV (7, 8) 6. SAP Full-length Yeast (9, 10) 7. BAL Catalytic dom. Human (11) 8. C-II Full-length Human (12) 9. SK Full-length Bacteria (13) 10. Plasminogen Cat-domain Human (14) 11. Cadosin A Full-length Plant (15) 12. Memapsin 2 Full-length Human (16-18) 13. Memapsin 1 Full-length Human 14. SAP1 Full-length C. albicans (19) 15. SAP2 Full-length C. albicans (19) 16. SAP3 Full-length C. albicans (19) 17. SAP6 Full-length C. albicans (19) 18. PreS Partial HBV 19. Cyclin A/CDK2-associated Full-length C. elegans protein P19 like 20. Sm protein motif Full-length C. elegans 21. Ubiquitin conjugating Full-length C. elegans protein 22. RNA binding region RNP-1 Full-length C. elegans 23. Sm protein motif Full length C. elegans SL1 trans-spliced S acidic Full-length C. elegans ribosomal protein P2 like 25. ARD/ARD family protein Full-length C. elegans
3 26. Mitochondrial precursor Full-length C. elegans receptor 27. Ubiquitin conjugating Full-length C. elegans enzyme 28. rho GDP dissociation Full-length C. elegans inhibitor 29. MIR domain motif Full-length C. elegans 30. GTP-binding Full-length C. elegans ADP-ribosylation factor 31. SM protein motif Full-length C. elegans yeast J0714 like 32. AF-9 protein like Full-length C. elegans 33. H+-transporting ATPase Full-length C. elegans 34. Archael histone like Full-length C. elegans 35. GCN3-transcription Full-length C. elegans activator 36. ALR Full-length Human 37. Fez1 Full-length Human 38. SMP30 Full-length Human 39. HOXC-8 Full-length Human 40. PRP24 Full-length Human 41. Rab5 Full-length Human 42. POMC Full-length Human 43. TCCR Full-length Human 44. CDC42 Full-length Human 45. ZNF230 Full-length Human 46. Pro-Insulin Full-length Human 47. VEGI Full-length Human 48. CSNK2B Full-length Human 49. GRAP2 Full-length Human 50. LCK Full-length Human 51. GRB2 Full-length Human 52. TGFBR2 Soluble domain Human 53. MMP14 Partial Human 54. MAPK11 Full-length Human 55. PPM1B Full-length Human
4 Description of the table: The refolded/purified proteins in #1-17 have been carefully studied and published. The purified proteins from are under structural and functional studies. The C. elegans proteins from are part of a structural genomic project aimed at purifying all C. elegans proteins, estimated to total about 19,000. These genes were randomly selected from C. elegans genome. References : 1. Lin, X., Wong, R. N. S., Tang, J. (1989) "Synthesis, purification, and active site mutagenesis of recombinant porcine pepsinogen". J. Biol. Chem. 264: Lin, X., Lin, Y., Koelsch, G., Gustchinas, A., Wlodawer, A., Tang, J. (1992) "Enzymic activities of tow-chair pepsinogen, two-chain pepsin, and the amino-terminal lobe of pepsinogen". J. Biol. Chem. 267: Lin, X., Loy, J. A., Sussman, F., Tang, J. (1993) "Conformational instability of the N- and C-terminal lobes of porcine pepsin in neutral and alkaline solutions". Prot. Sci. 2: Chen, Z., Koelsch, G., Han, H., Wang, X., Lin, X., Hartsuck, J. A., Tang, J. (1991) "Recombinant rhizopuspepsinogen Expression, Purification and activation properties of recombinant rhizopuspepsinogens". J. Biol. Chem. 266: Lin, Y., Fusek, M., Lin, X., Hartsuck, J. A., Kezdy, F. J., Tang, J. (1992) "ph dependence of kenetic parameters of pepsin, rhizopuspepsin, and their active-site hydrogen bond mutants". J. Biol. Chem. 267: Lin, X., Liu, M., Tang, J. (1992) "Heterologous expression of thermopsin, a heat-stable acid proteinase". Enzyme Microb. 14: Lin, Y., Lin, X., Hong,L., Foundling, S., Heinrikson, R. L., Thaisrivongs, S., Leelamanit, W., Raterman, D., Shaw, M., Dunn, B. M., Tang, J. (1995) "Effect of point mutations on the kinetics and inhibition of human immunodeficiency virus type 1 protease: relationship to drug resistance". Biochemistry 34: Ermolieff, J., Lin, X. Tang, J. (1997) "Kinetic properties of saquinavir-resistant mutants of human immunodeficiency virus type 1 protease and their implications in drug resistance in vivo". Biochem. 36: Lin, X., Tang, J., Koelsch, G., Monod, M., Foundling, S. (1993) "Recombinant canditropsin, and extracellular aspartic protease from yeast candida tropicalis". J. Biol. Chem. 268: Koelsch, G., Tang, J., Monod, M., Foundling, S. I., Lin, X in Aspartic Proteinases, "Primary substrate specificities of secreted aspartic proteases of candida albicans" James (Plenum Press, New York), pp Wang, C. S., Dashti, A. & Downs, D. (1999) " Bile salt-activated lipase Publication Types: Review, Review, tutorial". Methods Mol. Biol. 109:
5 12. Wang, C. S., Downs, D., Dashti, A. & Jackson, K. W. (1996) "Isolation and characterization of recombinant human apolipoprotein C-II expressed in Escherichia coli". Biochim Biophys Acta 1302: Wang, X., Lin, X., Loy, J. A., Tang, J., Zhang, X. C. (1998) "Crystal structure of the catalytic domain of human plasmin complexed with streptokinase". Science 281: Wang, X., Terzyan, S., Tang, J., Loy, J. A., Lin, X. & Zhang, X. C. (2000) "Human plasminogen catalytic domain undergoes an unusual conformational change upon activation". J Mol Biol 295: Faro, C., Ramal-Santos, M., Vieira, M., Mendes, A., Simoes, I., Andrade, R., Verissimo, P., Lin, X., Tang, J. & Peres, E. (1999) "Cloning and characterization of cdna encoding cardosin A, and RGD containing plant aspartic proteinase". J. Biol. Chem. 274: Lin, X., Koelsch, G., Wu, S., Downs, D., Dashti, A. & Tang, J. (2000) "Human aspartic protease memapsin 2 cleaves the secretase site of -amyloid precursor protein". Proc. Natl. Acad. Sci. USA 97: Ghosh, A. K., Shin, D., Downs, D., Koelsch, G., Lin, X., Ermolieff, J. & Tang, J. (2000) "Design of potent inhibitors for human brain memapsin 2 (beta-secretase)". J. Am. Chem. Soc. 122: Hong, L., Koelsch, G., Lin, L., Wu, S., Terzyan, S., Ghosh, A., Zhang, X. & Tang, J. (2000) "Structure of Memapsin 2 (beta-secretase) complexed with inhibitor: a template to design drugs for Alzheimer's Disease". Science 290: Koelsch, G., Tang, J., Loy, J. A., Monod, M., Jackson, K., Foundling, S. I. & Lin, X. (2000) "Enzymic characteristics of secreted aspartic proteases of Candida albicans". Biochim Biophys Acta 1480: Chew LJ, Pan H, Yu J, Tian S, Huang WQ, Zhang JY, Pang S, Li LY. A novel secreted splice variant of vascular endothelial cell growth inhibitor. FASEB J Mar Yu J, Tian S, Metheny-Barlow L, Chew LJ, Hayes AJ, Pan H, Yu GL, Li LY. Modulation of endothelial cell growth arrest and apoptosis by vascular endothelial growth inhibitor. Circ Res Dec 7;89(12): Zhai Y, Yu J, Iruela-Arispe L, Huang WQ, Wang Z, Hayes AJ, Lu J, Jiang G, Rojas L, Lippman ME, Ni J, Yu GL, Li LY. Inhibition of angiogenesis and breast cancer xenograft tumor growth by VEGI, a novel cytokine of the TNF superfamily. Int J Cancer Jul 2;82(1): Zhai Y, Ni J, Jiang GW, Lu J, Xing L, Lincoln C, Carter KC, Janat F, Kozak D, Xu S, Rojas L, Aggarwal BB, Ruben S, Li LY, Gentz R, Yu GL. VEGI, a novel cytokine of the tumor necrosis factor family, is an angiogenesis inhibitor that suppresses the growth of colon carcinomas in vivo. FASEB J Jan;13(1):181-9.
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