Atomic Theory: Spectroscopy and Flame Tests
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1 Atomic Theory: Spectroscopy and Flame Tests Pre-Lab Demonstrations: Gas Discharge Demo Your instructor will show samples of gas collected in thin glass tubes known as gas discharge tubes. The ends of the tubes have electrodes that allow a current to pass through the gas and light up. When inserted into a voltage source, the samples will glow a characteristic color. When the light is diffracted (e.g., with a prism), you can see the separate spectral lines that make up each sample. In the 2 boxes at the top of your Lab Report, color in the spectral lines that appear for the two gas samples your instructor will show. Diffraction of Light Through Stained Glass Now choose two colored panes of glass in the classroom. Predict what colors you expect to be present in each piece of glass. Now look at the panes of glass through the diffraction glasses. In the boxes on your Lab Report, draw the colors of light that are in the panes of glass. Were the panes of glass made up of the colors you predicted? Procedure: PART I. SPECTROPHOTOMETRIC ANALYSIS OF LIGHT Turn on the Spec-20 Genesys spectrophotometer, using the switch on the back side of the instrument; it will take about 15 minutes to warm up before use. On the top right of the instrument is a sample holder that holds the sample cells, called cuvettes. Never insert chemicals directly into the Spec-20, or you will contaminate and damage it. To the left of the sample holder is a panel of buttons that control the wavelength (in nanometers) and output (percent transmittance or absorbance) settings. The up/down arrows by wavelength turn the prism-like device in the Spec-20, so light of only one wavelength strikes the sample in the cuvette. Use the up/down arrows to adjust the wavelength to 400 nm. Your instructor will demonstrate how to place a piece of filter paper adjacent to the sample holder facing the light source. After you properly place the filter paper, stand where each student can see the side of the filter paper that faces the light source. You should see a tiny dot of color on the paper. Since the paper blocks the light rays from reaching the sample, the color observed is the color of light with a wavelength of 400 nm. Use the up/down arrows to scroll through wavelength values from 400 nm to 750 nm in 25 nm increments to find the colors corresponding to the different wavelengths of the visible spectrum. Use crayons or colored pencils to color in the spectrum in the box on your report sheet to show the correlation between color and wavelength. PART II: FLAME TESTS You will conduct flame tests to observe the flame emission colors for the following solutions: LiNO 3, Cu(NO 3 ) 2, Sr(NO 3 ) 2, Ba(NO 3 ) 2, KNO 3, and NaNO 3. Since nitrates do not emit color, you will be observing the color of the emission of the metallic cations. Safety precaution: Do not touch the chemicals on the splints with your fingers! Wash your hands immediately if you accidentally touch the chemicals on any of the splints. GCC CHM 151LL: Atomic Theory: Spectroscopy and Flame Tests GCC, 2008 page 1 of 6
2 1. Label your 6 test tubes with a pencil one for each solution listed above. From the reagent station, put about drops of each solution into the appropriate test tube. Place one splint into each test tube. These should soak for about 5 10 minutes in order to absorb enough solution. 2. At this time, each group should obtain one unknown from your instructor. Get a splint, and place it in the test tube of the unknown to allow sufficient time to soak. Be sure to record your unknown number on your Report Sheet. 3. Prepare your 150 ml beaker with about ml of water to dispose of the used splints. 4. Light your Bunsen burner with a striker. Your instructor will check to make sure that your flame is adjusted properly for the activity. You should see two blue cones of flame. 5. Grasp the LiNO 3 wood splint by the tip and place the damp end of the splint in the middle of the flame (in the tip of the inner cone) for a short time (about 2 3 seconds). You should see the color of the metal ion burning in the first few seconds. 6. Avoid burning the wood splint itself. If you start to notice the splint burning, immediately remove it from the flame. If it does start to burn, blow the flame out. You may immediately dip the non-burning splint back into the correct test tube and test the flame color again. You can repeat this several times if you have difficulty seeing the color. 7. When you are done testing a splint and its solution, dispose of the burned splint in the 150 ml beaker. 8. You can test the solutions in any order but we recommend the order above based on difficulty in seeing the colors of barium (Ba) and sodium (Na) ions. 9. Observe and carefully describe the color of the flame on the data table. For example, describe the color as pinkish red or violet red instead of just red. Use crayons to color in the corresponding flames in the data table. 10. Continue with the other solutions, recording the flame color for each solution. Be as descriptive, and accurate, as possible. 11. Observe and record the color given off by your unknown in the same manner. Identify the metal in your unknown solution. 12. When you have finished with all splints and solutions, dispose of all materials in the proper solid or liquid waste container in the hood. PART III. ABSORPTION AND EMISSION SPECTRA: A WEB TUTORIAL The third activity of this lab involves an interactive tutorial to help explain the process electrons go through when absorption and emission spectra are obtained from pure substances. Go to the Website below (a link is provided on the webpage for this week s experiment): Click on view tutorial for the second option, Section 3.3 Light Emission and Absorption (p ). Note the section numbers (e.g. Section 2 of 4 ) indicated at the bottom of each page during the tutorial. Be sure to click on the links for Real-World Connections, Science Connections, and Concept Questions during the tutorial. Answer the questions on Part III of your report sheet. You may refer back to each specific section in the tutorial as often as needed. GCC CHM 151LL: Atomic Theory: Spectroscopy and Flame Tests GCC, 2008 page 2 of 6
3 Atomic Theory: Spectroscopy and Flame Tests: Lab Report Name: Partner(s): Section Number: *Turn in all pages of the lab report!* Lab Notebook: Use the Section heading Pre-lab Gas Discharge Demo. Label Sample 1 on one line and Color of gas on the next line. These will be determined in lab. Below these lines draw a long rectangular box like the sample shown below. Label this box Spectral Lines (above or to the left of the box). Repeat for Sample 2. Spectral Lines Lab Notebook: Use the Section heading Light Through Stained Glass. List Glass 1 color on one 1 line and Predicted colors on the next. Below the Predicted colors line, draw a long rectangular box similar to the spectral lines, but label this box Stained Glass Colors. Pre-lab: Gas Discharge Demo Before putting the diffraction glasses on, identify the element being shown in the discharge tube and its color (be descriptive with the color). Now put the glasses on, and draw the appropriate colored diffraction lines with their correct placement. Pre-lab: Diffraction of Light Through Stained Glass Identify the two colored panes of glass in the classroom you want to analyze. Predict what colors you expect to be present in each piece of glass. Now look at the panes of glass through the diffraction glasses. Part I: Spectrophotometric Analysis of Light complete this on the handout (colors do not show up well in the notebooks). Visible light spectrum (shade in all colors between the numbers listed below): A spare table in case you mess up the first one. Visible light spectrum (shade in all colors between the numbers listed below): GCC CHM 151LL: Atomic Theory: Spectroscopy and Flame Tests GCC, 2008 page 3 of 6
4 Part II: Flame Tests Lab Notebook: Create a 4 column, 8 row table in your notebook. In the top row, label each column Sample, Substance, Color, Emission Color. Under Sample number each row 1, 2, through 6. Label the last row under Sample as Unknown. Under Substance, label each row LiNO 3, Cu(NO 3 ) 2, Sr(NO 3 ) 2, Ba(NO 3 ) 2, KNO 3, NaNO 3. Under Color, draw a circle in each row. You will color these in based on the colors of flames you observe. Under Emission Color, you will describe the color emitted in the flame test of each substance. Unknown #: Unknown substance: Part III: Online interactive tutorial: Select the Light Emission and Absorption link (2/3 of the way down the page). 1. (Section 1) Why do atoms of an element give off unique atomic spectra? 2. (Section 1) How are different colors of fireworks produced? 3. (Section 2) Describe the difference between the ground state and the excited state of an atom. 4. (Section 2) Describe what happens to an electron when it absorbs energy. Describe the movement of the electron when this happens. GCC CHM 151LL: Atomic Theory: Spectroscopy and Flame Tests GCC, 2008 page 4 of 6
5 5. (Section 2) Explain why hydrogen and sodium have different emission spectra. Questions and Calculations: (You must show all of your work on calculations to receive full credit.) 1. Determine if energy is absorbed or emitted for each electron transition in a hydrogen atom: a. from n = 4 to n = 2 emitted absorbed b. from n = 2 to n = 6 emitted absorbed c. from n = 6 to n = 9 emitted absorbed 2. In your data for Part I of the experiment, why did the color eventually disappear as you increased the wavelength? Does it mean there are no wavelengths past that point? Explain. 3. You are the student representative for Glendale Christmas fireworks display. Propose what chemicals should be used in the fireworks to have the fireworks match the theme. (Hint: Consider your flame test results.) 4. KSLX FM radio rocks out at MHz (radio station frequencies are in megahertz). Calculate the wavelength in meters for this Phoenix station. 5. Given that the sun is 93 million miles from Earth, how many minutes does it take light from the sun to reach Earth? (1 mile = km) 6. Which travels faster X rays or microwaves? Explain your answer. GCC CHM 151LL: Atomic Theory: Spectroscopy and Flame Tests GCC, 2008 page 5 of 6
6 7. Several Predators (aliens that see IR light or heat) have landed near your house one dark night, and Arnold is nowhere to be found. You need to gather supplies but do not want to turn on any light bulbs since they emit heat. Luckily, you find a black light that emits 5.45 x J/photon of energy. If Predators can only sense IR radiation, will the black light cause the Predators to be attracted to your house? Show all calculations and explain. 8. Fill in the blanks in this table of electromagnetic spectrum waves. Show your work below the table and on the back of the page. Watch units! Type of Frequency (Hz) Wavelength (nm) Energy (J) Wave Gamma ray 7.2 x 10-8 X ray 2.0 x TV wave 4.59 x Circle the correct answer for the following: a. Which has the higher frequency? Gamma ray or X ray b. Which has the shorter wavelength? TV wave or Gamma ray c. Which has the greater energy? TV wave or X ray 10. Use your calculations in the previous problem to fill in the following blanks: a. Frequency and energy are related. directly or inversely b. Wavelength and energy are related. directly or inversely c. Wavelength and frequency are related. directly or inversely GCC CHM 151LL: Atomic Theory: Spectroscopy and Flame Tests GCC, 2008 page 6 of 6
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