Differentiation of allergenic fungal spores by image analysis, with application to aerobiological counts
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1 15: , Kuw Puss. Pt t ts. 211 tt u ss y yss, wt t t uts.. By 1, S. s 2,EuR.Tvy 2 St 3 1 tt Ss, R 407 Bu (05), Uvsty Syy, SW, 2006, ust; 2 st ty, v 4 Bu u (6), sttut Rsty, Uvsty Syy, SW, 2006, ust; 3 SR tt t Ss, B 17, t Ry, SW, 1670, ust ( ut s: -: y@.usy.u.u; x: ) Rv 14 uy 1998; t 4 uy 1999 Ky ws: y st, u ss, yss, us, s y stt T ty yss ut t tt tw ss v u ws tst us yss s sv s u t 17 x tus, xtt ts s. u ss t, su, usu, sus, Pu, Btyts, Eu, Exsu, Ust, us Psy w x ss xts s t tt tw ss t t us ss v. ut st yss tu suts, 100 t 1600 ss tx, tt tw ss wt v uy. us ss us y sv s tus sut 98% uy t t t su, usu Eu. tt tw sus Pu ws t st, wt 56% sus ty t 41% st s Pu. t t ss v, su u, usu (),. xysu (),. s (), t t. ss w sstty t wt % uy. Ru vs uy t tt ss y yss t sts tw ss t s y. T t yss t ut ts s suss t t t suts t. 1. tut u ss sstty st t vt, tu vs vy t ss, t, u us, vtt t,.., vsus ut (E t., 1992; s, 1993; s, 1993; K, 1995, ; tz vt, 1996; tt t., 1997; ts t., 1997). T st vt u ss, y u t ut vts, ts t su (S Ry, 1979; B t., 1992, E t., 1992; K, 1995, ; tt t., 1997). w su v, ss t, sus, Pu, Eu, usu, Btyts, Exsu, Ust us st ts, vy s (s Ss, 1976; S Ry, 1979; Kz t., 1979; But t., 1984; B t., 1992; E t., 1992; Svstv Ww, 1992). t sss, us w t st ut stuy Tus,, wt ss s tu ty (vt, 1991). Sstst t u ss vs s t u t ty t u ts, u t sst t ss (ty) vs xsu t s. Ests t ts tw xsu ss us ut ts tt ut, y sssst. ut ts ssss sstst y s
2 212 tst wt u xtts, wst t tt ut u ss s ut ty us s vs. Rt s s uss t sssst sstst xsu, us s ss, ust yss, wt t v y ss (Tvy t., 1998; s t., 1998). tu tst s t tt ut u ss y yss. utt t u ss s vstt vusy t ut wtt u (s t., 1992). Ss suss w vw ytt u t t s ut us utt yss. By ts t u s s u ut tw uts, ty v t s uy t u t. T tt u ss t t v us ss t ss tus us yss s t ttt vusy. wv, ts wu xty usu t stus tt y u tt ut us t s, s t s uy tt u s t y xts wu u. ts stuy t ty yss tus t ty tt tw st u s sy t s y s vstt. T u st stuy w ts st y stus s s y u, wt vs s s, u t ss t, u ss su, t ss usu, Pu ysu T (sy. P. ttu Wst), sus utus, Eu u, Btyts Ps. x B., Exsu sttu (s) Sus (sy. tsu s s), Ust u (s) Rstu, us tus (u. x.) S.. y Psy uss (E) S. Ss Psy uss, v t tu y stus, ut xtts ts syt y ( t., 1995). 2. ts ts 2.1 u utus u utus ss w t s y w Sut Ws (T 1). u w utu u sts ts; 25 /20 12 tt, wt 12 t 75% tv uty t u wt. Ss t w utu V8 u, wst usu ss w utu t () t u w utu z Yst (Y). Ss w vst t u t 7 ys wt. Ss us tus, Psy uss Ust u w t ty t u ss. u ss w ut t ut u (50 t, 150 y st wt) s ss yss. usu w st 0.8% ty u, t ut t utt, t tt t tuy y. usu w t s t t s s t t ut t st, w y s 0.8% ty u wt x y t utt vs. t st ut ws w tw ts. t ss w ut t tu stt. 2.2 yss yss ws ut us yus B- 2 t s, sut v ( su xs t 256 y vs) WT ( Vs) ss stw. ut sty ts w us t su tt t t su t t ts t u ttu. Ss w t 50, , us t tv wt t ts s, u t s sz. suts w vt t ts y s vs ts. Ps tu xtt w v -us us t WT vt. tus xtt u ss y yss u 7 s tus; t, wt, wt:t t,, t, t uss; 17 x tus; uvt u t (E), tss, x, x t, us (. x.), t t, sty, vx u,
3 213 T 1. u ss, ss us utus us ts stuy, tt wt t u ss x y yss. Ss ss. S ty # Ss Ss t tt RR 2548 Su, u t ss R Ssyu t Puwu, SW u t t R Sw, T, SW u t t R tus sss, s, SW u t t R Suw,, SW u t t RR 5010 E. Ss, US u t s R tt, E, u t s R Ptt, tzy s, SW u sus utus RR 581 ty us, t, s u Btyts RR 2509 Ptt us, St, UK u su ss RR 3027 s, s u su u RR 2441 ts, y u su u RR 2459 tt s, ust u su u R 7 Sut, u, SW u su u RR 4198 s, ust u su ssu RR 4292, ust u us tus R Ptt, SW Bss Eu u R Su, Ptt, SW u Exsu sttu RR 3092 Su, ust u usu T, Vt u " " " u usu z s, ust u usu xysu 7396 t st, T u usu xysu 8455 S, w, T u usu s 7315 R st s, T u " " " u Pu ysu RR 821 yssus, wy u Psy uss R B, SW Bss Ust u R u, Sw Ts y ss sts: R = SW utu,, SW 2800; RR = vs S Ty, SR, t Ry, SW 2113; = usu Rs ty, Uvsty Syy, SW 2006; R = sttut Rsty, Uvsty Syy, SW # Ss s t u ss w suts w xtt y yss ss ss. vx u t, sty, vty, vxty, t wt (., v. x.) (T 2) (Russ, 1995). 2.3 s T s x ss t t u sts st T 1. Tus, t tt 1000 ss w x, su 1400 ss, usu 1600 ss, 200 ss t t w w st y s ss. y t sv s tu suts w us ts vstt t, s ss, w ys us t ut st yss. 2.4 Ss s Ss ss w ut t ss t, u ss su t ss usu, u st xts. sts x t u ss ys st T 1 s t tt (.) v Kss,. ss (B.) S.,. t wuy,. t Es P. Bss wtt,. t (s.) Tu s,. t Ss,. s Z.,. s (Es t) Su, su ss (s.) Vs,. u (Ps: s) x S.. y,. u Puss,. ssu Pz., usu S,. xysu St: s. s (tus) S.
4 214 T 2. tu suts xtt u ss y yss. tu u Uts t () xu t µ Wt (W) u t t t µ W Rt (WR) Wt t () u Pxs µ 2 t () 4π P 2 Pt (P) Pt t µ Russ (R) 4 π 2 Euvt u t (E) 4π µ tss () E Bx (BX) Bu t µ 2 Bx t (BXR) BX u us (R) u s us t t µ xu us (xr) xu us us t t µ t t (R) 2 R Sty (SP) R xr vx u () vx u µ 2 vx u t (P) Pt t vx u µ Sty (S) vty (v) µ 2 vxty (Vx) P P t () t xs µ v wt (W) Wt t xs µ wt (W) (wt t xs) µ x wt (xw) x (wt t xs) µ T st xt x ss su, usu t ss, s sust t stuy, us y t sv s tu suts. T u ss ys ts stuy sw T 1 s t u us ss s 1000 t, 1400 su 1600 usu ss. ut st yss ws t tu suts t. T s xt x ss su, usu t, wt 20 tus xtt. su usu ss w x us 200 ss (u usu), wst 100 ss w x t ss. T 20 tus xtt w; t, wt, wt:t t,, t, t, uss, E, tss, x, x t, us (. x.), t t, sty, vx u, vx u t, sty, vty vxty. y st yss ws t t suts t. t t xt y su usu ss w ys wt t s 20 tus xtt 200 ss ss, u usu ss. y st yss ws t t suts t. t ut xt t ss w x st, wt 100 ss ys 24 (7 + 17) tus xtt. T t u tus us ts vstt w t wt (., v. x.). y st yss ws t t suts t. 2.5 Sttst yss ut st yss w t t yss suts t t s, ss ss
5 215 T 3. Sv s tu suts (s) u ss t y yss. Ss ss # S t Wt W: Pt Russ ty µ µ t µ 2 µ t t tt RR t ss R t t R t t R t t R t t RR t s R t s R sus utus RR Btyts RR su ss RR su u RR su u RR su u R su u RR su ssu RR us tus R B Eu u R Exsu sttu RR usu " " usu usu xysu usu xysu usu s " " Pu ysu RR Psy uss R B Ust u R T y ss sts: R = SW utu,, SW 2800; RR = vs S Ty, SR, t Ry, SW 2113; = usu Rs ty, Uvsty Syy, SW 2006; R = sttut Rsty, Uvsty Syy, SW S Ty sts; = u, = u, = u, B = Bss, T = Ts. Ext 1. y st yss ws t t suts Exts 2, 3 4. st yss tts t tu s (.. t ut-s s w sut tu s tt s st xs s) t stt s tt st y t s ys, wst ut st yss s t s wt uv s ys. ut st s s ust t s t -v ut us tst t t ts x st uts. ts st stuy sut t ws v t ut yss t xt 1, ut y st yss ws t xts 2, Rsuts 3.1 suts y yss v suts t t sv s tus st T 3. stt sts w sv tw t ss t 23 ss t suts t, wt, t t t v wt:t t, t uss -
6 216 ts. t sts w sv tw t. tt,. t. t;. ssu. u;. utus P. ysu; Psy uss us tus; Ust u Btyts ;.. xysu.. s. Sts tw t ss t ss s t stt ts t t st tw st vs, tt y sw s v tw tx (us 1 2). st ts sts, sttst st vv st vs (.. t ust t st tw) sut vs st tw t vus tw t ss. 3.2 s ss t tus, sss tss t v t u us yss sv s tus ut st yss v v st tw (T 4). t su, Eu usu (t ) w stus t t ss wt 98% tt uy (T 4). Ss Psy, Exsu, Ust t w s t wt 92%, 89%, 86% 83% uy, stvy (T 4). Psy ss w sty t s us (4%) su (3%) ss, wst Ust ss w st t Btyts ss 12% ss. Exsu ss w st 11% ss s t ss, wst t ss w st s Exsu su ss 8% t t. tt w ut t ty u Btyts, Pu, us sus wt 69%, 67%, 63% 56% ss uty t, stvy. Btyts ss w st t st s Ust tss (27%) uty s su (4%). Pu sus w us y wt t, ut Pu w t wt t uy t sus (T 4). 3.3 Ss s t st xt x t ss t, u ss su t ss usu stuy T 4. s: suts ut st yss. us. t us ss us ss % (>2%) % t 8 83 su 8 Exsu 8 sus 1 56 Pu 41 Btyts 1 69 Ust 27 su 4 su 4 98 us 1 63 Psy 32 Eu 4 Eu 1 98 Exsu 1 89 t 11 usu 3 98 Pu 1 67 sus 32 Psy 1 92 us 4 su 3 Ust 1 86 Btyts 12 ss, wt y sv s tu suts, t sv ss w t wt vs s y ut st yss. t su u,. ssu, t t,. ss usu xysu. s.. xysu w t wt t t 90% uy (T 5). t t. u w t st y t ss y yss y sv tus, wt 97% 96% ss ty t, stvy. T st ut t tt, t ss y sv tus, w ts t. ss,. t. s wt 26%, 37% 47% ss t ty. t w t ty t 55% t t (T5). t s xt, w x su, usu t y, wt 20 tus xtt st yss tu suts, s w s s wt t st xt suts (T 5). T uy tt v t ss, ut t ss (T 5). T st t vts u t. ss,. t,. s,. t,.. s. tu t
7 217 B B B B B B B B B BB BB B K K K K E E E E E E EE EE E u 1. Stt t t st s sts -s uts ss t t s s y (sv s tus) ut st yss. t. sus. Btyts. su us. Eu. Exsu. usu. Pu. Psy. Ust t tts ( t ) st t tu us t w ss. w s ty s tts st ss tt v ty ss y yss. t tts st ss tt v sss y yss. T us t w sss ss v ss y yss s t t..
8 218 B B E E K K P P P P P R R R R R B B E E K K P P P P P R R R R R u 2. Stt t t st s sts -s uts ss t ss su, usu t s s y (20 tus) t Ext 2 st yss. t tt. t ss. t t. t t. t t. t t. t s. t s. su ss. su u. su u. su ssu. usu. usu. usu xysu. usu xysu. usu s. usu s t tts ( t ) st t tu ss t w ss. w s ty s tts st ss tt v ty ss y yss. t tts st ss tt v sss y yss. T ss t w sss ss v ss y yss s t t..
9 219 T 5. Ss ss: st su, usu t. Ss xt S yss t, su & t ss su usu ss usu ss y. tus st yss ut t tt %. ss u u ssu () () xysu () xysu () s () s () tt t t t ss t s s B u ts t st v tt uy v tt ss s ty. ws y 3% vt t tt. t, ts sut 100% uy tt ts ss, t st sut tt ts ss y t xts.. w s t wt t tst uy (85%) ts xt.. ss w uy w t (100%) ts xt wt 20 tus Ext 4 wt 24 tus y t ss. s t uy tt u t. t,. ssu,. tt,. u. xysu, Ext 1 t 2. T s uy t Ext Tw w y st y t v t ss t yss Ext T. T uy tt v ts yss st usu su ss. vts, Ext Tw, w st t t tt. ssu. ss. s. T st vs tt uy, wt t xts, w. ss,. u,. xysu t. s, ts xt wt 20 tus, wt t t tts 60, 99, 96, 95 94, stvy (T 5). t ut xt, w uss t ss y, wt t u st 24 tus, v t t ss v t st t ty t (T 5). t. ss w t wt 100% uy, wt t t ss. s (87%),. s,. t. t, wt 80% ty t y yss. uy tt t. tt,. t. t ws ss t ws v t t xts (T 5).
10 220 T 6. ssst ts y ut ts st yss. Ext ut s Ss Ext Ext Ext Ext Sttst yss ut st yss v t tst st tw ss Ext 1 t ss ss. T v ssst ts ss ss y t ut st yss st T 6. y 10.2% ss w sss y ut st yss, wt 15.9% sss y st yss. Sy, 25.9% ss su, usu t w sss y ut st yss, wt 33.8% y st yss Ext 1. ssst ts ss ss w wst t st su usu ss (Ext 3) wt 16.8% sss. T t ss ss u s ssst suts, wt 22.9% t sss Ext % su, usu t sss Ext 2. us 1 2 t t v st tw, ss t, su usu, stvy, s t st tw st vs y, s t s t ysy ss t t t st tw tx s vs, s 7 20 s s wu u ss, stvy. T sty s tw ss t tx s v y t xty t usts t tw-s s (us 1 2). u 1, t ust tts st usu (), Eu () su () ut stt ut v t t tx t Pu () sus (), t usts w s t s s t t t t. Sy, Ust () Btyts (), Psy () us (), t () Exsu () stut s tt, wt s v tw t. Ts us y ustt t suts st T 4, w usu, Eu su ss w t wt 98% uy, ut Pu sus ss w ut t tt, s w ss t Ust Btyts, Psy us, t Exsu. u 2, stt us vt, wt t usu ss (, ) ust tt t t t t. usu ss (, ) ust tt t tt t t, wst t s s v tw t s s t su ss;. ss (),. u (). ssu (), ust y (u 2). T ut su ss;. u () sssss ss w sy s sty wt ts t tt (),. t (). t (), w st- t ss. T vty s sz ss t. t s v y t s ts tx t. T - t ss;. ss (),. t (),. s (). s () v ust tt (t t), wt t sttv ss. t () s ut t t t ts u. Ts us y ustt t suts st Ext 2 T 5, w ss. t w t wt 100% uy, ut ss t. tt,. t,. t,. ss,. u. ssu w t wt ss t 63% uy. sutt t v sut t (T 3), tt wt xt suts (T 4 5) stt ts (us 1 2) w tu ust t sts s tw t ss t tx x. 4. suss T sut y sv s s ts tt st ss t sv t v vstt wt t t 82% uy. tt w -
11 221 ut t tt u sus, Pu, us Btyts wt t u ss sty t Pu, sus, Psy Ust, stvy. Ext t tu suts T 3 xs t ty t yss t tt w tw ss ts, t xsts t sty tw t s suts ts. R t tt tx ttss s t sty t s ts; t wt, tw t sttvs us ts stuy (Put Wtst, 1970; S Ttt, 1988; Ptt, 1991; K Ptt, 1994). wv, ss t Btyts Ust, t, su Exsu ssss sttv tsts w s y t u y tus, ss t ts wu v us wt t. tst, t s t sus tt sus Pu w us wt t y yss, s ts tus sttv t t u y. sv ss, t st vus t tt w t wt y sv tu suts, u. u,. ssu,. (),. xysu (),. tt. t. Ts s st t sts ts sv tw. ssu. u. tt. t. Ts ss. ss u v s, ts t s s sz, w uts t v tw ss (Es, 1971). T vty wt. u s t t suts T 3, w stt sty tw tw sts, wt t t ut t. Susy, xtt ut s ts sut v tt t ss, ut u uy t ss Ext 2. t s u tt uy, t u tu suts y v sut t vty t tst ss tus ss sty t. tst, v tt wu v sut t suts t xt tus vy ts t sv s tus, sut t sty w t tu suts. ut t u ss tv yss t y ss su usu Ext 3, sut v tt uy ss t sv ss wt t suts Ext 2. Ts s t t sut t ut t u ss t yss, u ss w t wt t tst uy ts xt. wv, vus t ss (. u,. ssu. xysu) w ty w t wt y sv s tu suts, s t ws t t xtt 20 tus ts ss st. T tt st t t ss ws ty v y t xtt 24 tus ss wt - ss. T u - s tys, t. ss,. t,. s. s w t wt tst s us 24 tu suts. t, t st- ss. t w s sy t y t 24 tu suts. wv, t s st- ss. tt. t w t wt tst uy y sv s tu suts. T uy yss uts v y t st tus t t yss. T t t us s ts w ys ut t ty t sst st tus t v st u ss s. (1995) t stuy usu suu ssu t sts y yss, u t u tus xtt sv s sz ts t ust t ts;, vxty uvtu. wv, st tw t t, t y s ss,. vtu,. tusu (B. ut.). suu u ssu stt (, 1995) ws y, wt u t 70%. tusu sss s. suu ssu stt. t. (1996), y uvtu s s ts (, vx, v ) t st tw t -t sus utus, uvu ut usu s utt syst. Ts stuy xts t w t. (1996), y y s yss ts t t tt st u -v ss s tx tt.
12 222 T t yss uts t t tt tt u ss ss wu u ut s us ty utus t ss w u t t tu, s t y t ts tt wu t v. Ts u t u ss st t vt, vty u t vt ts, t s ts tts t tts w ts ss tt ts u. ut yss ty ss u t x ss syts, yxyts Bsyts t sts t t t u vstt ts stuy. st, yss ut us s wu t t tt tw usu sss ts, s w s tt tw Ptys st- t. tt Sys Ttss ss y yss wu s t, s ts ss uty uty u t ut t ut. yss tus s y wu t. wv, t s y tt v wt t xtt t u tus, wt t wu sttst yss, ts ts w v 100 t ut t ts. Ts y vstt t t ty yss t tt tw st u u ss ty utus u ss s stt t tt ts tu, ut ut s s u tt t. wts T uts wu t t Pst, SW utu,, v Bus, usu Rs ty, Uvsty Syy, s Ptt s, SR, t Ry t suy u utus ss us ts stuy. Rs B., s., tt., S. u.: 1992, tv stuy ut ss Pu. 8, But., Ku.., Sut.. Vs K.: 1984, vut- stuy ss uus v s uts ws stt tts v tst ts.. y 53, tz V. vt E.: 1996, T s sut ss Tus,. 12, Es.B.: 1971, tus yyts. B tt: W, U.K E.R., swt K..: 1992, ut u s t w ttu vts. y. Rs. 2, tt.., B..,....: 1997, u ss 80 s t t Vy, ust: vs, ssty -ut ts. 13, B..: 1995, ut- t yss usu suu u ssu t sts. yt 129, s S..: 1993, u t ts t s. 32, s S.R. Ss..: 1976, -y suvy wt s twv s.. y 36, s.: 1993, Rts tw u s s wt vs. 32, W.E.,., Sv.E. S.B.: 1995, u s... Rv. 8, s.s., By..., s. Tvy E.R.: 1998, T R y ts st t. y y, st uy, 54t u t, Wst stt. s..,.t. w.e.: 1992, T us yss s uts wt-t u. Bt. T. 6(5), K.. Ptt..: 1994, ty u t sus Ss T Ts. SR, vs Pss Kz P.P., u., us.. S..: 1979, ts t t t v s.. y 43, vt E.: 1991, tt tt sss. 30, W. K B.: 1995, y-u s u ss ut. y 87(2), W. K B.: 1995, y-u ut y stuy Wt, t,. 34, ts T., E.K., Stvs., ust. Kx R.B.: 1997, su, t tt u ss t ts u (ust) v t ys. 13, : 1995, tt usu suu u ssu stt,. tusu (B. ut.). v.. vtu s. v. yt 129, K-B., Y., tsu., Yt. Kut.: 1996, t u s t y ut- yss ts t t tu tvty vut.. Bty 45, Ptt..: 1991, ty u t Pu Ss. SR, vs Pss Put E. Wtst..: 1970,... sts Pt u Bt. 280; Ust u. wt utu Buux, t Bt.
13 223 Russ..: 1995, T Pss, 2. R Pss: B Rt,. S.. Ttt..: 1988, uss Tsts ust. t Pss: u. S.R. Ry R.R.: 1979, u ss t t s tts utu uty.. 12-t stuy ut s.. y 43, Svstv.K. Ww K.: 1992, ss stts t s. 31, Tvy E.,., T., W., v U. s.: 1998, w vt ust t. y y, st uy, 54t u t, Wst stt.
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