Comparing Several Means: ANOVA. Group Means and Grand Mean
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1 STAT 511 ANOVA and Regressin 1 Cmparing Several Means: ANOVA Slide 1 Blue Lake snap beans were grwn in 12 pen-tp chambers which are subject t 4 treatments 3 each with O 3 and SO 2 present/absent. The ttal yield was measured fr each chamber. Sulfur Dixide Ozne Absent resent Absent resent T cmpare the means f several say I grups (ppulatins) ne ften uses an analysis f variance mdel r ANOVA. Fr the I ppulatins we use µ 1 µ 2... µ I and σ 1 σ 2... σ I t dente their respective means and standard deviatins. Similarly the sample mean sample standard deviatin and sample size f the ith ppulatin are dented by x i s i and J i. Of mst interest are the cmparisns between the µ i s. Grup Means and Grand Mean Slide 2 Fr the bean grwth data trt J i j x ij x i The grand ttal f n = 12 bservatins is i j x ij = s the grand mean is x = = The J i s here are all equal s x is the mean f x i s. This wuld nt be the case fr J i s unequal. Fr J i s large by CLT X i N(µ i σ2 i J i ) and s 2 i are reliable estimates f σ 2 i. Fr J i s small ne assumes nrmality and σ 2 1 = = σ 2 I = σ 2. The individual sample means are x i = 1 J i Ji j=1 x ij where x ij is the jth bservatin in the ith grup. The grand mean is x = 1 n I i=1 Ji j=1 x ij where n = I i=1 J i is the ttal number f bservatins in the I grups.
2 STAT 511 ANOVA and Regressin 2 Variatin Within Grups Slide 3 Fr the bean grwth data trt j (x ij x i ) 2 s 2 i SSE is i j (x ij x i ) 2 = and MSE is s 2 p = = Fr J i s all equal s 2 p = i s2 i/i. In general s 2 p is a weighted mean f s 2 i with weights (J i 1). Under the assumptin σ 2 1 = = σ 2 I = σ 2 ne wuld like t estimate the cmmn variance σ 2 using all available infrmatin. Such infrmatin is cntained in the sum f squared errrs SSE = I Ji i=1 j=1 (x ij x i ) 2 = I i=1 (J i 1)s 2 i. The pled variance estimate is given by s 2 p = MSE = SSE n I where n I = I i=1 (J i 1). Variatin Between Grups Slide 4 Fr the bean grwth data SSTr is given by i 3( x i x ) 2 = and SST is given by i j (x ij x ) 2 = It is easy t verify that SST = SSTr + SSE If ne ignres the gruping then the sample variance f the n bservatins is s 2 = 1 n 1 SST. T measure the variability between grups ne calculates the sum f squares fr treatments SSTr = I Ji i=1 j=1 ( x i x ) 2 = I i=1 J i( x i x ) 2. It can be shwn that i j (x ij x ) 2 = i j (x ij x i ) 2 + i j ( x i x ) 2 where SST = i j (x ij x ) 2. Fr I = 2 it can be shwn that SSTr = ( x 1 x 2 ) 2 1 J J 2
3 STAT 511 ANOVA and Regressin 3 ANOVA Table F-Test Slide 5 Assciated with SSE and SST are degrees f freedm n I and n 1. Similarly SSTr has df I 1. Nte that n 1 = (n I) + (I 1). Dividing SS by the crrespnding df ne gets a mean square (MS). An ANOVA table summarizes all the infrmatin. Src SS df MS Trt SSTr I 1 Errr SSE n I Ttal SST n 1 SSTr I 1 SSE n I MSE is an unbiased estimate f σ 2. Fr µ i s all equal MSTr is als an unbiased estimate f σ 2. When µ i s are nt all equal MSTr tends t be larger. T test the hyptheses H 0 : µ 1 = = µ I Calculate vs. H a :.w. f = MSTr MSE and reject H 0 when f > F αν1 ν 2 where ν 1 = I 1 and ν 2 = n I. F-Distributin Slide 6 Let Y i N(0 1) i = 1... m and Z j N(0 1) j = 1... n independent. The distributin f m i=1 Y i 2 /m n j=1 Z2 j /n is called a F-distributin with degrees f freedm ν n = m and ν d = n. F(38) and F(83) Fr the bean grwth data the ANOVA table is given by Src SS df MS Trt Errr Ttal It is easy t calculate f = = which is larger than F.0538 = 4.07 s we reject H 0 at the 5% significance level. T btain F.0538 qf(.9538). in R use
4 STAT 511 ANOVA and Regressin 4 F- and t-tests Cmputing Frmulas Slide 7 Fr I = 2 ne has f = MSTr MSE = ( x 1 x 2 ) 2 s 2 p( 1 J J 2 ). Reject H 0 when f > F α1n 2. Cmpare this with the t-test fr H 0 : µ 1 = µ 2 versus H a : µ 1 µ 2 t = x 1 x 2 q 1 s p J J 2 with a rejectin regin t > t α/2n 2. We ntice that f = t 2. Actually ne als has F α1ν = t 2 α/2ν s the F-test is equivalent t the t-test we learned earlier. Since SST = SSTr+SSE ne nly needs t calculate tw f the three terms. SST = i j (x ij x ) 2 j x2 ij ( i j x ij) 2 n = i SSTr = i j ( x i x ) 2 ( j x ij) 2 J i ( = i SSE = i j (x ij x i ) 2 = i j x2 ij i i j x ij) 2 n ( j x ij) 2 J i. Cmputing ANOVA: Example Slide 8 Cnsider the fllwing data Sample J i j x ij j x2 ij x i n = 9 i j x ij = 54 x = 6. 4 Using the cmputing frmulas SSE = ( ) = 24 SSTr = ( ) = 66. Since f = 66/2 24/6 = 8.25 and F.0526 = 5.14 we reject H 0 : µ 1 = µ 2 = µ 3 at the 5% significance level.
5 STAT 511 ANOVA and Regressin 5 arameter Estimatin and Testing Slide 9 Fr the bean grwth data x 1 = x 2 = s 2 p =.0344 = J 1 = J 2 = 3 ν = 8. A 95% CI fr µ 1 is q ± r ( ) where t.0258 = The inferences cncerning means are derived frm the fact that X i N(µ i σ2 J i ). A (1 α)100% CI fr µ i is s s x i ± t 2 p α/2ν J i where ν = n I. A (1 α)100% CI fr µ 1 µ 2 is A 95% CI fr µ 1 µ 2 is q.17 ± 2.306(.1855) 2 3 r ( ). One wuld accept H 0 : µ 1 = µ 2 at the 5% level. ( x 1 x 2 ) ± t α/2ν rs 2 p( 1 J J 2 ) Tests fr hyptheses cncerning these parameters can be similarly cnstructed. Estimating and Testing Cntrasts Slide 10 Fr the bean grwth data a cntrast f interest is θ = (µ 1 µ 2 ) (µ 3 µ 4 ). θ = 0 implies n interactin between O 3 and SO 2. The estimate is given by ˆθ = x 1 x 2 x 3 + x 4 =.47 with a standard errr ˆσˆθ =.1855 p 4/3 = A 95% CI fr θ is.47 ± 2.306(.2142) r ( ). One wuld cnclude θ = 0 at the 5% level. A linear cmbinatin f means θ = c 1 µ c I µ I is t be estimated by ˆθ = c 1 x c k x I with a standard errr ˆσˆθ = s p s c 2 1 J c2 I J I. When c 1... c I add t zer i c i = 0 such a θ is called a cntrast. Fr example µ 1 µ 2 is a cntrast. In applicatins cntrasts are ften f the mst interest.
6 STAT 511 ANOVA and Regressin 6 Slide 11 Relatins Between Variables Functinal relatins: y = f(x) deterministic such as (i) A = πr 2 fr the area A and radius r f a circle; r (ii) y = 5 9 (x 32) fr thermmeter readings x F and y C. Statistical relatins: Variables tend t vary tgether but there is n deterministic cupling. Amng examples are (i) ages f married cuples; and (ii) lengths and weights f snakes. radius area length (cm) weight (gm) Slide 12 Simple Linear Regressin When studying the heights f father-sn pairs Galtn fund in late 19th century that fr fathers taller than average the average height f their sns is between their height and the average. Ditt fr fathers shrter than average. A simple linear regressin is f the frm Y = β 0 + β 1 x + ǫ Y respnse r dependent var. x predictr r indep. var. ǫ nise r randm errr Y varies randmly given x. The distributin f Y varies systematically with x thrugh the regressin functin µ Y x = β 0 + β 1 x. The mdel has a systematic part β 0 + β 1 x and a randm part ǫ. A causal structure is usually implied.
7 STAT 511 ANOVA and Regressin 7 Mdel Assumptins in SLR Data cme in as pairs (x i y i ) and the mdel is written as Y i = β 0 + β 1 x i + ǫ i It is usually assumed that ǫ i N(0σ 2 ). Slide 13 Cnsider Y = x + ǫ where ǫ N(0 9). Since Y x=1 N(20 9) ne has In practice ne bserves pairs (x i y i ) and estimates mdel parameters β 0 β 1 and σ 2. µ Y x = β 0 + β 1 x is a strng assumptin. (Y <17 x= 1) = (Z < ) = The nrmality assumptin can smetimes be weakened t µ ǫi = 0 and σ 2 ǫ i = σ 2. Example: Length and Weight f Snakes Slide 14 Length Weight Nine adult females f the snake Vipera berus were caught and measured. The lengths and weights are listed n the left and pltted belw. weight (gm) length (cm)
8 STAT 511 ANOVA and Regressin 8 Least Squares Estimates f β 0 β 1 Slide 15 The lengths and weights f female snakes. The LS estimate f regressin functin is Y = X. weight (gm) Y= X Y=-227+6X length (cm) Q= Q=1347 Minimizing w.r.t. β 0 β 1 Q = nx (y i (β 0 + β 1 x i )) 2 i=1 ne btains the least squares (LS) estimates f (β 0 β 1 ) where b 1 = ˆβ 1 = S xy b 0 = ˆβ 0 = ȳ b 1 x. S xy = i (x i x)(y i ȳ) = i (x i x) 2. Fitted Values and Residuals Slide 16 The lengths and weights f female snakes. x y ŷ e The mean respnse µ Y x at x is (unbiasedly) estimated by the fitted regressin functin ˆµ Y x = Ŷ = b 0 + b 1 x. At the data pints ne has the fitted values (y-hat) and the residuals ŷ i = b 0 + b 1 x i e i = y i ŷ i = y i (b 0 + b 1 x i ). The fitted values and residuals satisfy n i=1 ŷi = n i=1 y i n i=1 e i = n i=1 x ie i = 0.
9 STAT 511 ANOVA and Regressin 9 Estimatin f σ 2 Slide 17 Cnsider a mdel Y i = µ + ǫ i where µ ǫi = 0 and σ 2 ǫ i = σ 2. The estimate ŷ i = ˆµ = ȳ actually minimizes Q = n i=1 (y i µ) 2. An unbiased estimate f σ 2 is n s 2 i=1 = (y i ŷ i ) 2 n 1 n i=1 = e2 i n 1 where ŷ i cntains ne parameter. T estimate σ 2 calculate the residual sum f squares nx nx SSE = (y i ŷ i ) 2 = e 2 i and use i=1 s 2 = SSE n 2 = i=1 i (y i ŷ i ) 2. n 2 Unbiasedness: µ s 2 = σ 2. T calculate s 2 use where SSE = S yy S2 xy S yy = i (y i ȳ) 2. Details f Calculatin We use the lengths and weights f snakes t illustrate. Nte that S xy = X xi yi x i y i = X x 2 i ( x i ) 2. n n Slide 18 First summarize the data. xi = 567 x 2 i = yi = 1368 y 2 i = xi y i = Then calculate x = = 63 ȳ = = 152 = = 172 S yy = = 9990 S xy = (1368) 9 = Nw we have b 1 = = 7.19 b 0 = (63) = 301 SSE is given by = s σ 2 is estimated by s 2 = =
10 STAT 511 ANOVA and Regressin 10 Inferences Cncerning β 1 Lengths and weights f snakes. Assume ǫ i N(0 σ 2 ). Slide 19 We have b 1 = 7.19 and q s b1 = = A 95% CI fr β 1 is given by 7.19 ± 2.365(.953) where t.0257 = T test the hyptheses b 1 N(β 1 σ 2 b 1 ) where σ 2 b 1 = σ 2 / is t be estimated by s 2 b 1 = s2. The inferences are based n H 0 : β 1 = 0 vs. H a : β 1 0 we calculate t = = and reject H 0 even at the 1%- level as t > = t b 1 β 1 s b1 t n 2. Fr example a (1 α)100% CI fr β 1 is given by b 1 ± t α/2n 2 s b1. Analysis f Variance Slide 20 The lengths and weights f female snakes. Surce SS df MS F Mdel Resid Ttal Decmpse the deviatin f y i frm ȳ y i ȳ = (ŷ i ȳ) + (y i ŷ i ) where (ŷ i ȳ) is systematic and (y i ŷ i ) is randm. It can be shwn that i (y i ȳ) 2 = i (ŷ i ȳ) 2 + i (y i ŷ i ) 2 SST : (n 1) = SSR : 1 + SSE : (n 2) The ANOVA table summarizes related infrmatin. Surce SS df MS f Mdel SSR 1 SSR 1 Resid SSE n 2 s 2 = SSE n 2 Ttal SST n 1 MSR MSE
11 STAT 511 ANOVA and Regressin 11 F-Test fr β 1 = 0 Slide 21 The lengths and weights f female snakes. Since f = = F.0117 = we reject H 0 : β 1 = 0 at the 1% level. This is equivalent t the t-test n Slide 19. Nte that It can be shwn that µ MSR = σ 2 + β 2 1 µ MSE = σ 2. When β 1 = 0 ne has f = MSR MSE F 1n 2. These lead t the F-test fr H 0 : β 1 = 0 vs. H a : β 1 0 which rejects H 0 when F s > F α1n 2. The F- and t-tests are equivalent: f = = = t 2 F.0117 = = = t MSR MSE = f = t2 = ( b 1 s b1 ) 2 F α1n 2 = t 2 α/2n 2. Inferences Cncerning β 0 Slide 22 Fr the lengths and weights f snakes β 0 has n meaning. Cnsider Y = 15+5X +ǫ where ǫ N(0 4). Given x i = 8(.1)10 simulate Y i and estimate the regressin functin. Assume ǫ i N(0 σ 2 ). where b 0 N(β 0 σ 2 b 0 ) σ 2 b 0 = σ 2 { 1 n + x2 } is t be estimated by s 2 b 0 = s 2 { 1 n + x2 } The inferences are based n b 0 β 0 s b0 t n 2. Fr x large β 0 is hard t estimate r t interpret.
12 STAT 511 ANOVA and Regressin 12 Inferences Cncerning µ Y x = β 0 + β 1 x Slide 23 The lengths and weights f female snakes. We are t estimate the average weight f snakes f length 60 cm. Ŷ = (60) = s 2 Ŷ = {1 9 + (60 63)2 172 } = = Assume ǫ i N(0 σ 2 ). Ŷ N(β 0 + β 1 x σ 2 Ŷ ) where Ŷ = b 0 + b 1 X and σ 2 Ŷ = σ2 { 1 n + (x x)2 } is t be estimated by s 2 Ŷ = s2 { 1 n + (x x)2 }. The inferences are based n s a 95% CI fr β 0 + β 1 60 is ± 2.365(5.053) r ( ). Ŷ (β 0 + β 1 x) sŷ t n 2. Fr x x large β 0 +β 1 x is hard t estimate. redictin f New Observatin Slide 24 The lengths and weights f female snakes. We are t predict the weight f a snake f length 60 cm. Ŷ = s 2 = s 2 Ŷ = T predict a new respnse at x Y = β 0 + β 1 x + ǫ ne has t allw fr the variability f ǫ. With β 0 β 1 and σ 2 knwn the predictin interval (β 0 + β 1 x) ± z α/2 σ cvers Y with prbability 1 α. s a 95% I fr Y at X = 60 is ± r ( ). This is wider than the CI fr β 0 + β With β 0 + β 1 x estimated by Ŷ = b 0 + b 1 x we use Ŷ ± t α/2n 2 qs 2 + s 2 Ŷ where the variances f Ŷ and ǫ are estimated by s 2 and Ŷ s2.
13 STAT 511 ANOVA and Regressin 13 Slide 25 R 2 Crrelatin Lengths and weights f snakes. R 2 = =.891 r = (9990) =.944 The cefficient f determinatin r R 2 R 2 = SSR SST = 1 SSE SST measures the amunt f variatin explained by the mdel. The cefficient f crrelatin r = S xy p Sxx S yy measures the linear assciatin between X and Y. 0 R r 1. R 2 = r 2.
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