Lung-residing myeloid-derived suppressors display dual functionality in murine. pulmonary tuberculosis
|
|
- Amie Norris
- 6 years ago
- Views:
Transcription
1 Lung-residing myeloid-derived suppressors display dual functionality in murine pulmonary tuberculosis Julia K. Knaul, Sabine Jörg, Dagmar Oberbeck-Mueller, Ellen Heinemann, Lisa Scheuermann, Volker Brinkmann, Hans-Joachim Mollenkopf, Vladimir Yeremeev, Stefan H.E. Kaufmann, nca Dorhoi This Online Data Supplement includes: Supplementary Methods Supplementary Figure legends E1 E8
2 Supplementary Methods Reagents used in flow cytometry studies For phenotypic characterizations the following antibodies were used: anti-cd16/cd3 (4G, TCC) anti-cd19 (1D3, TCC), anti-cd3 (145-C11, TCC), anti-nk1.1 (PK136, TCC), anti-nkp46 (91.4, ebioscience, Frankfurt, Germany), anti-ly6g (18, BD), anti-ly6c (L-1, BD), anti-cd14 (mil4r-m1, BD), anti-cd11b (M1/7, BD), anti-cd11c (N418, TCC or BD), anti-gr1 (RB6-8C5, BD), CD86 (GL-1, BioLegend), anti-cd31 (MEC 13.3, BD), anti-mhcii (Tib1, TCC), anti-mhci (B.49, TCC) anti-cd49d (R1-, ebioscience), anti-cd115 (FS98, ebioscience) and anti-f4/8 (BM8, ebioscience), anti-cd3 (UCHT1, BD), anti-cd4 (191, TCC), anti- CD8 (53-6.7, BD). For intracellular cytokine staining the following antibodies were used: anti-tnfα(xt-,tcc) anti-ifnγ (XMG1., BD) and anti-il17(n49-653, BD). To guarantee consistency between experiments, gates were set using fluorescence minus one controls on whole lung (i.e. not fractionated) before being applied to fractionated samples. t least cells were acquired on a FCS-CantoII or LSRII (BD) and analyzed by FlowJo software (Tree Star Inc., shland, OR, US). Cytological analysis Cytospins of BM-MDSCs were generated using a CytoSpin III centrifuge (Thermo Scientific Shandon, Hudson, NH, US). Samples were stained by modified Giemsa (Differential Quick Stain Kit, Polyscience Inc., Warrington, P, US). t least cells from five microscopic fields were quantified to assess cell morphology. E
3 nalysis of co-culture supernatants Chemokine and cytokine concentrations were analyzed using immunoassay (Bio-Rad, Munich, Germany), commercial TNFα or IL-1 ELIS (R&D Systems, Minneapolis, MN, US) or in-house IL-6 ELIS. Cell death was assessed by detecting lactate dehydrogenase levels in co-culture supernatants (Roche, Basel, Switzerland). E3
4 Supplementary Figures E1-E4 Figure E1. Gene expression and cytokine abundance in lung homogenates/sera from TB-resistant and -susceptible inbred mouse strains Expression of MDSC-associated genes in lungs of infected C57BL/6 and 19S mice at day 7 p.i. () and day 14 p.i. (B). Fold expression + indicates upregulation in 19S mice; expression denotes elevated expression in C57BL/6 mice. Fold change calculated as: fold change in C57BL/6 over fold change in 19S lungs. (C) Cytokine levels in lung and serum at day 5 p.i. in C57BL/6 and 19S mice. (D) Gene expression of selected molecules allowing distinction between MDSCs and tumorassociated neutrophils in Mtb-infected C57BL/6 and 19S animals (MDSC profile: fold expression < to ; tumor-associated neutrophil profile: fold change < or >+). ( D) Values from three independent experiments, each with five specimens per mouse strain, median±iqr, Mann Whitney test. **** P.1 Figure E. Gating strategy applied for flow cytometry analysis Single cell suspensions from whole lung or MCS-purified cells were analyzed by 8- color flow cytometry. Leukocytes were selected before all dump + B cells (CD19 + ), T cells (CD3 + ), NK/NKT cells (NK1.1 + and/or Nkp46 + ) were excluded from the analysis. Subsequently, dendritic cells and alveolar macrophages (CD11c + ) were eliminated from the analysis. CD11b + Ly6G + cells and CD11b + Ly6G cell subsets were selected and the latter was then further gated on Ly6C expression. Finally, both CD11b + Ly6G + and CD11b + Ly6G Ly6C + cells were analyzed for marker expression. E4
5 (B) Representative plots for CD11b and Gr1 expression on unfractionated lung cells after exclusion of Dump + and CD11c + cells. (C) Representative plots for CD14 expression throughout the course of Mtb infection. Due to the heightened TBsusceptibilty of the 19S strain, analysis for days 8 and 6 p.i. could not be performed. ( C) Data from one representative of two independent experiments; n=1. Figure E3. Gr1 + cell analysis of unsorted lung cells during TB Single cell suspensions from lungs were analyzed by flow cytometry without magnetic cell isolation at day 7, 14 or 1 p.i. with Mtb. () Ly6G + Gr1 int cell number and CD14- expression. (B) nalysis of Ly6G Gr1 high cell number and marker CD14 expression. Data from two independent experiments (n=1, mean±sem, two-way NOV with Bonferroni s post-test) **** P.1, ** P.1. Figure E4. Lung Gr1 + cells do not supress TNFα and IL-17 responses ex vivo. () TNFα production of splenocytes in the presence of Ly6G + Gr1 int (left) and Ly6G Gr1 high (right) cells isolated from lungs at day 14 p.i. (top) and day 1 p.i. (bottom). (B) IL17F (top) and IL-17 (bottom) production of splenocytes in the presence or absence of Ly6G + Gr1 int (left) and Ly6G Gr1 high (right) cells isolated from lungs at day 1 p.i.. (, B) Results are from two independent experiments (n=1, mean±sem), one-way NOV with Bonferroni s post-test. (C) TNFα production (left) and cell death (right) of splenocytes in the presence or absence of either MDSCs subset or PMNs isolated from lungs of 19S animals at day 1 p.i. Results from two independent experiments (n=4 7, median±iqr Mann-Whitney t-test). (D) TNFα production of splenocytes in the E5
6 presence or absence of BM-MDSCs or BM-PMNs from C57BL/6 mice infected with Mtb. Results from three independent experiments, which three replicas each (median±iqr Mann-Whitney t-test). ** P.1, ** P.1, * P.5. Figure E5. BCG-induced response in MDSCs is comparable to Mtb-induced effects. () Frequency of BCG + macrophages and Gr1 + cells subsets. (B) Concentration of cytokines produced by BCG-infected BM-MDSCs or BM-macrophages 4 h p.i. (, B) Data from two independent experiments, with three replicates each (median±iqr, Mann-Whitney t-test). ** P.1, * P.5. Figure E6. Cytologic and phenotypic characterization of in vitro-generated MDSCs BM-derived MDSCs were generated as previously described (3). () Giemsa stain of BM-derived MDSCs (n=5 mean±sem). (B D) Phenotypic analysis of BM-derived MDSC subsets. Data from four to seven independent experiments. Figure E7. BM-MDSCs do not supress TNFα responses. () TNFα production of polyclonally stimulated splenocytes in the absence or presence of naïve or infected BM-derived MDSCs. Data from one representative of three to five independent experiments with three replicates each; mean±sem, one-way NOV with Bonferroni s post-test. (B) TNFα production (top) and cell death (bottom) of polyclonally stimulated splenocytes in the absence or presence of infected BM-derived MDSCs from WT, Nos /, rg1 / and Nos / rg1 / animals. Data from three independent E6
7 experiments with duplicates each (mean±sem). **** P.1, *** P.1, ** P.1, ** P.5. Figure E8. blation of MDSCs ameliorates pathology in TB 19S mice received TR or Placebo during Mtb-infection and were analyzed at day 1 p.i. () Frequencies and numbers of lung CD11c + cells (n=6, mean±sem,). (B) Cell numbers of lung CD49d +, CD86 +, MHCII +, CD115 + and CD14 + CD11b + Gr1 + (n=6, mean±sem). (C) Immunostaining for MPO (red) and nuclei (blue) and (D) for NOS (green), rg1 (red) and nuclei (blue) of lung parenchyma (n=1). (C) Scale bar 1 µm (top) and 1 µm (bottom), n=1. (D) Scale bar 1 µm (top/bottom), n=1. (E) IFNγ (left) and TNFα (right) production and (F) cell death of polyclonally stimulated splenocytes in co-cultures with mycobacteria-infected BM-derived MDSCs treated with TR/DMSO for 5 days. (G) Immunostaining for CD3 (red) and Ki67 (green) and nuclei (blue) (n=6, scale bar 1 µm). (-D, G) Data from two independent experiments. (E-G) Data from two independent experiments with duplicates each (mean±sem). **** P.1, *** P.1, ** P.1, * P.5. E7
8 fo ld c h a n g e C 5 7 B L /6 v s. 1 9 S lu n g c o n c e n tra tio n (p g /m l) s e ru m c o n c e n tra tio n (p g /m l) fo ld c h a n g e B 6 v s. 1 9 S fo ld c h a n g e B 6 v s. 1 9 S S u p d a y 7 lu n g m ic ro a rra y B C C 5 7 B L /6 u p S ta t3 S ta t5 b S ta t5 a Irf8 P u S u p C 5 7 B L /6 u p S ta t3 S ta t5 b S ta t5 a C 5 7 B L /6 1 9 S I L -6 P lc y S ta t6 C /e b p C /e b p Il-6 Il-6 ra G -c s f d a y 1 4 lu n g m ic ro a rra y Irf8 P u.1 P lc y S ta t6 C /e b p C /e b p Il-6 Il-6 ra G -c s f G -C S F G m -c s f M -c s f P ro k C /e b p S 1 a 8 S 1 a 9 IL -1 Id o G m -c s f M -c s f P ro k C /e b p S 1 a 8 S 1 a 9 Il-1 Id o Il-4 Il-4 ra P ro k 1 P ro k r1 P ro k r T d o C d 4 4 M m p 9 C 5 7 B L /6 1 9 S I L -6 Il-4 Il-4 ra P ro k 1 P ro k r1 P ro k r T d o C d 4 4 M m p 9 G -C S F S u p d a y 7 d a y 1 4 d a y 5 D C 5 7 B L /6 u p L R ik C c l8 C c l1 rg 1 C c l7 D a b C h 5 h M m p 1 3 S p p 1 C x c l4 E a r C 8 R ik C x c l1 6 Figure E1
9 19S B6 Gr1 19S 19S C57BL/6 Leukocytes Non-dump CD11c- Ly6G+ Ly6C+ nalysis of marker expression B Day 14 Day 1 14% 37% 13% 16% CD11b C FMO control Day Day 7 Day 14 Day 1 Day 8 Day 6,%,7% 7% 13% 47,5% ND ND C57BL/6 Ly6G,4% 1,3% 4,8% 9,5% 14% 6,5% 14% CD14 Figure E
10 L y 6 G - G r1 h ig h c e lls (x 1 5 ) L y 6 G - G r1 h ig h C D c e lls (% ) L y 6 G + G r1 in t c e lls (x 1 6 ) L y 6 G + G r1 in t C D c e lls (% ) L y 6 G + G r1 in t % C D C 5 7 B L /6 1 9 S 1 8 C 5 7 B L /6 1 9 S * * D a y s p o s t in fe c tio n D a y s p o s t in fe c tio n B L y 6 G - G r1 h ig h % C D C 5 7 B L /6 1 9 S 1 C 5 7 B L /6 1 9 S D a y s p o s t in fe c tio n D a y s p o s t in fe c tio n Figure E3
11 T N F (% re le a s e ) L a c ta te d e h y d ro g e n a s e (O D 4 9 ) T N F (% re le a s e ) IL -1 7 (% re le a s e ) IL -1 7 (% re le a s e ) IL -1 7 F (% re le a s e ) IL -1 7 F (% re le a s e ) T N F (% re le a s e ) T N F (% re le a s e ) T N F (% re le a s e ) T N F (% re le a s e ) Ly6G + Gr1 int cells Ly6G - Gr1 high cells C 5 7 B L /6 1 9 S C 5 7 B L /6 1 9 S : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G + G r1 in t : S p le n o c y te s 1 : 1 : :1 1 : 1 : :1 L y 6 G - G r1 h ig h : S p le n o c y te s C 5 7 B L /6 1 9 S C 5 7 B L /6 1 9 S : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G + G r1 in t : S p le n o c y te s 1 : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G - G r1 h ig h :S p le n o c y te s B Ly6G + Gr1 int cells Ly6G - Gr1 high cells 6 C 5 7 B L /6 1 9 S 1 5 C 5 7 B L /6 1 9 S * 4 1 * * 5 1 : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G + G r1 in t : S p le n o c y te s 1 : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G - G r1 h ig h : S p le n o c y te s 6 4 C 5 7 B L /6 1 9 S * * C 5 7 B L /6 1 9 S * 6 4 * * C * * 1 : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G + G r1 in t : S p le n o c y te s M D S C s P M N s S p le n o c y te s L y 6 G + G r1 in t M D S C L y 6 G - G r1 h ig h M D S C L y 6 G + P M N S p le n o c y te s 1 : 1 : 1 :4 1 :8 :1 1 : 1 : 1 :4 1 :8 :1 L y 6 G - G r1 h ig h : S p le n o c y te s D 3 1 L y 6 G + G r1 in t Figure E4 L y 6 G - G r1 h ig h L y 6 G + S p le n o c y te s. 1 : 1 :1 1 : 1 :1 1 : 1 :1 :1 C e ll s u b s e t : S p le n o c y te M D S C s P M N s S plenocytes
12 CD31 CD14 CD49d % o f c o u n te d c e lls CD115 MHCII MHCI F4/8 CD11c Day Day 4 B ll cells C Gr1 high CD11b high Gr1 low CD11b high Gr1 dim CD11b int ll singlets M o n o c y tic P o ly m o rp h o n u c le a r CD11b+ Gr1+ Subsets 1- Gr1 high CD11b high - Gr1 low CD11b high 3- Gr1 int CD11b int D a y D a y 4 D G r 1 h i g h C D 1 1 b h i g h G r 1 lo w C D 1 1 b h i g h G r 1 in t C D 1 1 b lo w C D 1 1 c F 4 /8 M H C I M H C II C D 4 9 d C D 1 4 C D 3 1 C D Figure E5
13 G r1 h ig h C D 1 1 b h ig h G r1 d im C D 1 1 b h ig h G r1 lo w C D 1 1 b in t M a c ro p h a g e s B C G + c e lls (% ) S e c re te d C y to k in e (n g /m l) B M D S C s, B C G M a c ro p h a g e s, B C G * * * * * IL -1 (O D 4 5 ) T N F IL - 6 IL - 1. Figure E6
14 L a c ta te d e h y d ro g e n a s e (O D 4 9 ) T N F (% re le a s e ) T N F (p g /m l) 4 S p le n o c y te s o n ly C o -c u ltu re -M tb C o -c u ltu re + M tb M D S C o n ly 3 * * * 1 * * B 6 4 :1 1 : 1 :4 1 :8 1 :1 6 1 : 1 :4 1 :8 1 :1 6 1 : 1 : - M tb + M tb G r1 + C D 1 1 b + c e lls : S p le n o c y te s S p le n o c y te s o n ly W T M D S C s N o s - /- M D S C s rg 1 - /- M D S C s N o s - /- rg 1 - /- M D S C s :1 1 : 1 :1 1 : 1 :4 1 : 1 :1 1 : 1 :4 1 : 1 :1 1 : 1 :4 1 : 1 :1 1 : 1 :4 G r1 + C D 1 1 b + c e lls : S p le n o c y te s 3 S p le n o c y te s o n ly W T M D S C s N o s - /- M D S C s rg 1 - /- M D S C s N o s - /- rg 1 - /- M D S C s 1 :1 1 : 1 :1 1 : 1 :4 1 : 1 :1 1 : 1 :4 1 : 1 :1 1 : 1 :4 1 : 1 :1 1 : 1 :4 G r1 + C D 1 1 b + c e lls : S p le n o c y te s Figure E7
15 L a c ta te d e h y d ro g e n a s e (O D 4 9 ) Placebo T N F (% re le a s e ) TR IF N (% re le a s e ) C D 1 1 c + L e u k o c y te s (% ) C D 1 1 b + G r1 + ce lls (x 1 6 ) T R P la c e b o T R P la c e b o C D 1 1 c + c e lls (x1 6 ) B 4 T R P la c e b o 3 1 C D TR Placebo TR Placebo C D 4 9 d C D 8 6 M H C II C D C D 1 4 E D M S O M T R S p le n o c y te s G * * * 5 n.d. n.d. 1 : 1 : 1 :4 1 : 1 : 1 :4 :1 M D S C : S p le n o c y te 6 D M S O M T R S p le n o c y te s 4 * * * * * 1 : 1 : 1 :4 1 : 1 : 1 :4 :1 F 1. M D S C : S p le n o c y te D M S O S p le n o c y te s M T R : 1 : 1 :4 1 : 1 : 1 :4 :1 Figure E8 M D S C : S p le n o c y te
Actinobacteria Relative abundance (%) Co-housed CD300f WT. CD300f KO. Colon length (cm) Day 9. Microscopic inflammation score
y groups y individuals 9 Actinobacteria Relative abundance (%) acteroidetes Cyanobacteria Deferribacteres Firmicutes Proteobacteria TM Tenericutes Unclassified CDf CDf Co-housed CDf Co-housed CDf CDf CDf
More informationStandardized and flexible eight colour flow cytometry panels harmonized between different. laboratories to study human NK cell phenotype and function
Standardized and flexible eight colour flow cytometry panels harmonized between different laboratories to study human NK cell phenotype and function John P Veluchamy 1,2, María Delso-Vallejo 3, Nina Kok
More informationFSC-W FSC-H CD4 CD62-L
Supplementary Fig. 1 a SSC-A FSC-A FSC-W FSC-H SSC-W SSC-H CD4 CD62-L b SSC-A FSC-A FSC-W FSC-A FSC-A 7-AAD FSC-A CD4 IL-9 CD4 c SSC-A FSC-A FSC-W FSC-H SSC-W SSC-H 7-AAD KI67 Annexin-V 7-AAD d I L -5
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature11419 Supplementary Figure 1 Schematic representation of innate immune signaling pathways induced by intracellular Salmonella in cultured macrophages. a, During the infection Salmonella
More informationM e a n. F l u o r e s c e n c e I n t e n s i t y P r o t e i n ( p g / m L )
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0. 0. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 P r o t e i n ( p g / m L ) 0. 0. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 P r o t e i n ( p g / m L ) Figure. Representative
More informationClass Diagrams. CSC 440/540: Software Engineering Slide #1
Class Diagrams CSC 440/540: Software Engineering Slide # Topics. Design class diagrams (DCDs) 2. DCD development process 3. Associations and Attributes 4. Dependencies 5. Composition and Constraints 6.
More informationCJ LI LRRK2 MOUSE COMPARISON STUDY. Phenotyping Data Results
CJ LI MOUSE COMPARISON STUDY Phenotyping Data Results NOTE FOR USE This comparison study was run as an opportunistic look at the phenotype of multiple CJ Li-generated mouse lines. The primary purpose of
More informationc. What is the average rate of change of f on the interval [, ]? Answer: d. What is a local minimum value of f? Answer: 5 e. On what interval(s) is f
Essential Skills Chapter f ( x + h) f ( x ). Simplifying the difference quotient Section. h f ( x + h) f ( x ) Example: For f ( x) = 4x 4 x, find and simplify completely. h Answer: 4 8x 4 h. Finding the
More informationSTEEL PIPE NIPPLE BLACK AND GALVANIZED
Price Sheet Effective August 09, 2018 Supersedes CWN-218 A Member of The Phoenix Forge Group CapProducts LTD. Phone: 519-482-5000 Fax: 519-482-7728 Toll Free: 800-265-5586 www.capproducts.com www.capitolcamco.com
More informationARC 202L. Not e s : I n s t r u c t o r s : D e J a r n e t t, L i n, O r t e n b e r g, P a n g, P r i t c h a r d - S c h m i t z b e r g e r
ARC 202L C A L I F O R N I A S T A T E P O L Y T E C H N I C U N I V E R S I T Y D E P A R T M E N T O F A R C H I T E C T U R E A R C 2 0 2 L - A R C H I T E C T U R A L S T U D I O W I N T E R Q U A
More informationA L A BA M A L A W R E V IE W
A L A BA M A L A W R E V IE W Volume 52 Fall 2000 Number 1 B E F O R E D I S A B I L I T Y C I V I L R I G HT S : C I V I L W A R P E N S I O N S A N D TH E P O L I T I C S O F D I S A B I L I T Y I N
More informationgender mains treaming in Polis h practice
gender mains treaming in Polis h practice B E R L IN, 1 9-2 1 T H A P R IL, 2 O O 7 Gender mains treaming at national level Parliament 25 % of women in S ejm (Lower Chamber) 16 % of women in S enat (Upper
More informationSUPPLEMENTARY INFORMATION
GP2 Type I-piliated bacteria FAE M cell M cell pocket idc T cell mdc Generation of antigenspecific T cells Induction of antigen-specific mucosal immune response Supplementary Figure 1 Schematic diagram
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature09414 Supplementary Figure 1 FACS-isolated 8c hepatocytes are highly pure. a, Gating strategy for identifying hepatocyte populations based on DNA content. b, Detection of mchry and mchr9
More informationM a n a g e m e n t o f H y d ra u lic F ra c tu rin g D a ta
M a n a g e m e n t o f H y d ra u lic F ra c tu rin g D a ta M a rc h 2 0 1 5, A n n a F ilip p o v a a n d J e re m y E a d e 1 W h a t is H y d ra u lic F ra c tu rin g? Im a g e : h ttp ://w w w.h
More informationSupplementary material
Supplementary material Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins Vladimir V. Rogov 1,*, Hironori Suzuki 2,3,*, Mija Marinković 4, Verena Lang
More informationForm and content. Iowa Research Online. University of Iowa. Ann A Rahim Khan University of Iowa. Theses and Dissertations
University of Iowa Iowa Research Online Theses and Dissertations 1979 Form and content Ann A Rahim Khan University of Iowa Posted with permission of the author. This thesis is available at Iowa Research
More informationHemolysis and Immune Activation. Karina Yazdanbakhsh, PhD Executive Director, Research Institute New York Blood Center
Hemolysis and Immune Activation Karina Yazdanbakhsh, PhD Executive Director, Research Institute New York Blood Center Hemolysis in Sickle Cell Disease (SCD) Hemolysis activates the underlying endothelium:
More informationSupplementary Figure 1-10 with Figure legends
332 coordinates mechanotransduction and growth cone bifurcation in sensory neurons Li Yang Chiang 1, Kate Poole, Beatriz E. Oliveira, Neuza Duarte,Yinth Andrea Bernal Sierra, Leena Bruckner Tuderman, Manuel
More informationC o r p o r a t e l i f e i n A n c i e n t I n d i a e x p r e s s e d i t s e l f
C H A P T E R I G E N E S I S A N D GROWTH OF G U IL D S C o r p o r a t e l i f e i n A n c i e n t I n d i a e x p r e s s e d i t s e l f i n a v a r i e t y o f f o r m s - s o c i a l, r e l i g i
More informationSupplementary Figure 1 Analysis of beige fat and cells and characteristics of exosome release, related to Figure 1
Supplementary Figure 1 Analysis of beige fat and cells and characteristics of exosome release, related to Figure 1 (a) Fold-change in UCP-1 mrna abundance in white adipocytes upon β-adrenergic stimulation
More informationCELL REPLICATION. Fluorescent light microscopy showing mitosis, especially immunolabelled cytoskeleton and tubulin
CELL REPLICATION Fluorescent light microscopy showing mitosis, especially immunolabelled cytoskeleton and tubulin Cell REPLICATION PROLIFERATION MUTIPLICATION DIVISION CELL REPLICATION Fluorescent light
More informationA homo-dimer of annexin V protects against ischemia reperfusion injury in lung transplantation
A homo-dimer of annexin V protects against ischemia reperfusion injury in lung transplantation K Hashimoto, H Kim, H Oishi, M Chen, I Iskender, J Sakamoto, A Ohsumi, Z Guan, DM Hwang, TK Waddell, M Cypel,
More informationPIERIS PHARMACEUTICALS, INC. (Exact Name of Registrant as Specified in its Charter)
UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of Report (Date of earliest event
More informationEKOLOGIE EN SYSTEMATIEK. T h is p a p e r n o t to be c i t e d w ith o u t p r i o r r e f e r e n c e to th e a u th o r. PRIMARY PRODUCTIVITY.
EKOLOGIE EN SYSTEMATIEK Ç.I.P.S. MATHEMATICAL MODEL OF THE POLLUTION IN NORT H SEA. TECHNICAL REPORT 1971/O : B i o l. I T h is p a p e r n o t to be c i t e d w ith o u t p r i o r r e f e r e n c e to
More informationMitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization
Cell Metabolism Supplemental Information Mitochondrial Dynamics Is a Distinguishing Feature of Skeletal Muscle Fiber Types and Regulates Organellar Compartmentalization Prashant Mishra, Grigor Varuzhanyan,
More informationAlita Miller, PhD. Head of BioScience. April 24, 2017 ECCMID 2017 Vienna, Austria
ETX5 restoration of sulbactam activity against multidrug resistant Acinetobacter baumannii correlates with β-lactamase inhibition in vitro and in vivo Alita Miller, PhD Head of BioScience April, 07 ECCMID
More informationBeechwood Music Department Staff
Beechwood Music Department Staff MRS SARAH KERSHAW - HEAD OF MUSIC S a ra h K e rs h a w t r a i n e d a t t h e R oy a l We ls h C o l le g e of M u s i c a n d D ra m a w h e re s h e ob t a i n e d
More informationMeasuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer
Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer Nexcelom Bioscience LLC. 360 Merrimack Street, Building 9 Lawrence, MA 01843 T: 978.327.5340 F: 978.327.5341
More informationLU N C H IN C LU D E D
Week 1 M o n d a y J a n u a ry 7 - C o lo u rs o f th e R a in b o w W e w ill b e k ic k in g o ff th e h o lid a y s w ith a d a y fu ll o f c o lo u r! J o in u s fo r a ra n g e o f a rt, s p o rt
More informationby the detector. However, the ion
Guidelines for Masss Cytometry Panel Design 1 Mass Cytometry combines the advantages of single cell high speed analysis common to conventional flow cytometry with the ability to resolve over 90 metal probes
More informationS U E K E AY S S H A R O N T IM B E R W IN D M A R T Z -PA U L L IN. Carlisle Franklin Springboro. Clearcreek TWP. Middletown. Turtlecreek TWP.
F R A N K L IN M A D IS O N S U E R O B E R T LE IC H T Y A LY C E C H A M B E R L A IN T W IN C R E E K M A R T Z -PA U L L IN C O R A O W E N M E A D O W L A R K W R E N N LA N T IS R E D R O B IN F
More informationheliozoan Zoo flagellated holotrichs peritrichs hypotrichs Euplots, Aspidisca Amoeba Thecamoeba Pleuromonas Bodo, Monosiga
Figures 7 to 16 : brief phenetic classification of microfauna in activated sludge The considered taxonomic hierarchy is : Kingdom: animal Sub kingdom Branch Class Sub class Order Family Genus Sub kingdom
More information600 Billy Smith Road, Athens, VT
600 Billy Smith Road, Athens, VT Curtis Trousdale, Owner, Broker, Realtor Cell: 802-233-5589 curtis@preferredpropertiesvt.com 2004 Williston Road, South Burlington VT 05403 www.preferredpropertiesvt.com
More informationUNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C Form 8-K/A (Amendment No. 2)
UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K/A (Amendment No. 2) Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of Report
More informationPhoto. EPRI s Power System and Railroad Electromagnetic Compatibility Handbook
Photo EPRI s Power System and Railroad Electromagnetic Compatibility Handbook Brian Cramer Project Manager Transmission and Substations bcramer@epri.com 815/478-5344 Problem Periodic false activation of
More informationThe Ability C ongress held at the Shoreham Hotel Decem ber 29 to 31, was a reco rd breaker for winter C ongresses.
The Ability C ongress held at the Shoreham Hotel Decem ber 29 to 31, was a reco rd breaker for winter C ongresses. Attended by m ore than 3 00 people, all seem ed delighted, with the lectu res and sem
More informationInfluence of Bacterial Growth Rate on Dose Optimization of Linezolid for Treatment of Tuberculosis
Influence of Bacterial Growth Rate on Dose Optimization of Linezolid for Treatment of Tuberculosis Kristina Bigelow Johns Hopkins University School of Medicine 1/15/217 Linezolid Recently added to WHOs
More informationSupplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice Supplementary Figure 2.
Supplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice. Percentage of marginal zone B cells in the spleen of wild-type mice (+/+), mice homozygous for cpm or pri
More informationLife in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae
Life in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae Frederik Schulz, Ilias Lagkouvardos, Florian Wascher, Karin Aistleitner, Rok Kostanjšek, Matthias Horn Supplementary
More informationDesign Principles for Maximizing the Drug Delivery Efficiency and Therapeutic Index of ADCs
Design Principles for Maximizing the Drug Delivery Efficiency and Therapeutic Index of ADCs World ADC 217 San Diego Robert Lyon Sr. Director, Chemistry Themes of the presentation This represents the desired
More informationSupplementary Figure 1. SDS-PAGE analysis of GFP oligomer variants with different linkers. Oligomer mixtures were applied to a PAGE gel containing
Supplementary Figure 1. SDS-PAGE analysis of GFP oligomer variants with different linkers. Oligomer mixtures were applied to a PAGE gel containing 0.1% SDS without boiling. The gel was analyzed by a fluorescent
More informationHuman C-reactive protein ELISA using Sword Peroxidase Reagents
Human C-reactive protein ELISA using Sword Peroxidase Reagents Enhanced performance using the Tecan Infinite M200 multimode reader Introduction Human C-reactive protein C-reactive protein (CRP) is a homopentameric
More informationCharacterization of the Murine T-Lymphocyte Response to Salmonella enterica Serovar Typhimurium Infection
INFECTION AND IMMUNITY, Jan. 2002, p. 199 203 Vol. 70, No. 1 0019-9567/02/$04.00 0 DOI: 10.1128/IAI.70.1.199 203.2002 Copyright 2002, American Society for Microbiology. All Rights Reserved. Characterization
More informationTable of contents Appendix Figure S1 Appendix Figure S2 Appendix Figure S3 Appendix Table S1 Appendix Table S2
Table of contents Appendix Figure S1 Appendix Figure S2 Appendix Figure S3 Appendix Table S1 Appendix Table S2 1 Appendix Figure S1 Fig. S1. Proteome analysis of the CPE-derived EVs and the choroid plexus
More informationNucView TM 488 Caspase-3 Assay Kit for Live Cells
NucView TM 488 Caspase-3 Assay Kit for Live Cells Catalog Number: 30029 (100-500 assays) Contact Information Address: Biotium, Inc. 3423 Investment Blvd. Suite 8 Hayward, CA 94545 USA Telephone: (510)
More informationValidation of the GastroPlus TM Software Tool and Applications
Validation of the GastroPlus TM Software Tool and Applications Fagen Zhang and Leah Luna The Dow Chemical Company FZ/MB 01.11.11 Acknowledgements Michael Bartels Barun Bhhatarai (Novartis) Tyler Auernhammer
More informationSupplementary Methods
Supplementary Methods Modeling of magnetic field In this study, the magnetic field was generated with N52 grade nickel-plated neodymium block magnets (K&J Magnetics). The residual flux density of the magnets
More informationAGRICULTURE SYLLABUS
Agriculture Forms 1-4.qxp_Layout 1 26/10/2016 12:29 PM Page 1 ZIMBABWE MInISTRY OF PRIMARY AnD SECOnDARY EDUCATIOn AGRICULTURE SYLLABUS FORM 1-4 2015-2022 Curriculum Development and Technical Services,
More informationTHE BANK OF NEW YORK MELLON CORPORATION (Exact name of registrant as specified in its charter)
UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of Report (Date of earliest event
More informationX-ray Absorption at the Near-edge and Its Applications
X-ray Absorption at the Near-edge and Its Applications Faisal M Alamgir faisal@msegatechedu School of Materials Science and Engineering, Georgia Institute of Technology Cartoon of XAS ln(i 0 /I t ) or
More informationSUPPLEMENTARY FIGURES AND TABLES AND THEIR LEGENDS. Transient infection of the zebrafish notochord triggers chronic inflammation
SUPPLEMENTARY FIGURES AND TABLES AND THEIR LEGENDS Transient infection of the zebrafish notochord triggers chronic inflammation Mai Nguyen-Chi 1,2,5, Quang Tien Phan 1,2,5, Catherine Gonzalez 1,2, Jean-François
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/7/345/ra91/dc1 Supplementary Materials for TGF-β induced epithelial-to-mesenchymal transition proceeds through stepwise activation of multiple feedback loops Jingyu
More informationPreparing Colloidal Gold for Electron Microscopy
Corporate Headquarters 400 Valley Road Warrington, PA 18976 1-800-523-2575 FAX 1-800-343-3291 Email: info@polysciences.com www.polysciences.com Europe - Germany Polysciences Europe GmbH Handelsstr. 3 D-69214
More information1Q ,836 2Q ,738 3Q ,758 4Q ,147
A N A LY S T M E E T I N G 2 Q 2 0 1 1 1 9 A u g u s t 2 0 1 1 S e m i n a r R o o m, 1 6 / F S a n s i r i P u b l i c C o m p a n y L i m i t e d A g e n d a P r o j e c t L a u n c h P r e s a l e s
More informationAPPH 4200 Physics of Fluids
APPH 42 Physics of Fluids Problem Solving and Vorticity (Ch. 5) 1.!! Quick Review 2.! Vorticity 3.! Kelvin s Theorem 4.! Examples 1 How to solve fluid problems? (Like those in textbook) Ç"Tt=l I $T1P#(
More informationLSU Historical Dissertations and Theses
Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1976 Infestation of Root Nodules of Soybean by Larvae of the Bean Leaf Beetle, Cerotoma Trifurcata
More informationUse precise language and domain-specific vocabulary to inform about or explain the topic. CCSS.ELA-LITERACY.WHST D
Lesson seven What is a chemical reaction? Science Constructing Explanations, Engaging in Argument and Obtaining, Evaluating, and Communicating Information ENGLISH LANGUAGE ARTS Reading Informational Text,
More information5 questions, 3 points each, 15 points total possible. 26 Fe Cu Ni Co Pd Ag Ru 101.
Physical Chemistry II Lab CHEM 4644 spring 2017 final exam KEY 5 questions, 3 points each, 15 points total possible h = 6.626 10-34 J s c = 3.00 10 8 m/s 1 GHz = 10 9 s -1. B= h 8π 2 I ν= 1 2 π k μ 6 P
More informationInverse Problem for Dynamical Systems with Uncertain and Scarce Data DAVID SWIGON UNIVERSITY OF PITTSBURGH
Inverse Problem for Dynamical Systems with Uncertain and Scarce Data DAVID SWIGON UNIVERSITY OF PITTSBURGH Immune response to IAV infection: Experimental design Intranasal infection of Balb/c mice by Influenza
More informationBrain Neurosecretory Cytokines. Immune Response and Neuronal Survival
Brain Neurosecretory Cytokines Immune Response and Neuronal Survival Library ofcongress Cataloging-in-Publication Data Brain neurosecretory cytokines : immune response and neuronal survival / edited by
More informationNature Neuroscience: doi: /nn.2717
Supplementary Fig. 1. Dendrite length is not secondary to body length. Dendrite growth proceeds independently of the rate of body growth and decreases in rate in adults. n 20 on dendrite measurement, n
More informationA new ThermicSol product
A new ThermicSol product Double-Faced Thermo-Electric Solar-Panel TD/PV & Solar Tracker & Rotation Device An EU-patent protected product TP4-referens.pdf D o y o u w a n t to c o n v e rt it i n to G re
More informationNK cells are part of the innate immune response. Early response to injury and infection
NK cells are part of the innate immune response Early response to injury and infection Functions: Natural Killer (NK) Cells. Cytolysis: killing infected or damaged cells 2. Cytokine production: IFNγ, GM-CSF,
More informationGrain Reserves, Volatility and the WTO
Grain Reserves, Volatility and the WTO Sophia Murphy Institute for Agriculture and Trade Policy www.iatp.org Is v o la tility a b a d th in g? De pe n d s o n w h e re yo u s it (pro d uc e r, tra d e
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Expression of GCaMP6s in LGN and their axons in V1.
Supplementary Figure 1 Expression of GCaMP6s in LGN and their axons in V1. (a, b) Coronal section of LGN and V1 expressing GCaMP6s. a Coronal slice (bregma -2.3 mm) including thalamus confirmed that GCaMP6s
More informationOrgan-on-a-chip: practical applications & challenges. Remko van Vught
Organ-on-a-chip: practical applications & challenges Remko van Vught 10-4-2018 A leap forward in physiological relevance The challenge of reductionism: Make things as simple as possible, but not simpler,
More informationRayBio Human CD86/B7-2 ELISA Kit
RayBio Human CD86/B7-2 ELISA Kit Catalog #: ELH-CD86 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationImmunoassay Kit (Colorimetric)
RayBio cgmp Direct Immunoassay Kit (Colorimetric) User Manual Version 1.0 May 25, 2014 RayBio cgmp Direct Immunoassay Kit (Colorimetric) Protocol (Cat#: 68AT-cGMP-S100) RayBiotech, Inc. We Provide You
More informationSoftware Architecture. CSC 440: Software Engineering Slide #1
Software Architecture CSC 440: Software Engineering Slide #1 Topics 1. What is software architecture? 2. Why do we need software architecture? 3. Architectural principles 4. UML package diagrams 5. Software
More informationLactate Dehydrogenase Assay Kit
Lactate Dehydrogenase Assay Kit Catalog Number KA0878 500 assays Version: 10 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationSupplementary Figure 1: To test the role of mir-17~92 in orthologous genetic model of ADPKD, we generated Ksp/Cre;Pkd1 F/F (Pkd1-KO) and Ksp/Cre;Pkd1
Supplementary Figure 1: To test the role of mir-17~92 in orthologous genetic model of ADPKD, we generated Ksp/Cre;Pkd1 F/F (Pkd1-KO) and Ksp/Cre;Pkd1 F/F ;mir-17~92 F/F (Pkd1-miR-17~92KO) mice. (A) Q-PCR
More informationSupplementary Figure 1. AnnexinV FITC and Sytox orange staining in wild type, Nlrp3 /, ASC / and casp1/11 / TEC treated with TNF /CHX.
Supplementary Figure 1. AnnexinV FITC and Sytox orange staining in wild type, Nlrp3 /, ASC / and casp1/11 / TEC treated with TNF /CHX. Phase contrast and widefield fluorescence microscopy (20x magnification).
More informationCertificate Sound reduction of building elements
Certificate Sound reduction of building elements Test report 040311.Z01b * ) Applicant GLAVERBEL GROUP Chaussée de la Hulpe 166 B- 1170 Brussels Specimen Monolithic glazing Foundation EN 20140-3 : 1995-01
More informationFunctional expression of PEPT2 and its regulation in alveolar epithelial cells
Workshop on Drug Transporters in the Lungs TCD, Dublin, IRL, Sept. 22-23, 216 Functional expression of PEPT2 and its regulation in alveolar epithelial cells Mikihisa Takano, Ph.D. Graduate School of Biomedical
More informationMySQL 5.1. Past, Present and Future. Jan Kneschke MySQL AB
MySQL 5.1 Past, Present and Future Jan Kneschke MySQL AB Agenda Past S Q L T re e s m e e ts D y n a m ic S Q L P re s e n t E v e n ts P a rtitio n in g F u tu re V e rtic a l P a rtitio n in g About
More informationRayBio Human Semaphorin 6D ELISA Kit
RayBio Human Semaphorin 6D ELISA Kit Catalog #: ELH-SEMA6D User Manual Last revised February 9, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100
More informationCharacterizing degradation kinetics and cellular mechanisms of PROTAC compounds. Kristin M. Riching, Ph.D. Protein Degradation Therapeutics 2018
Characterizing degradation kinetics and cellular mechanisms of PROTAC compounds Kristin M. Riching, Ph.D. Protein Degradation Therapeutics 28 Understanding the Dynamics and Mechanisms of Protein Degradation
More informationHuman anti-ige receptor antibody ELISA Kit
Human anti-ige receptor antibody ELISA Kit Catalog No. MBS702743 (96 T) This immunoassay kit allows for the in vitro semi-quantitative determination of human anti-ige receptor antibody concentrations in
More informationActivation of STING with Synthetic Cyclic Dinucleotides and Synergy with Checkpoint Inhibition
Activation of STING with Synthetic Cyclic Dinucleotides and Synergy with Checkpoint Inhibition Sarah McWhirter, PhD Director, STING Program Aduro Biotech ICI Boston 2017 Presentation 1 Disclosures Sarah
More informationMOLINA HEALTHCARE, INC. (Exact name of registrant as specified in its charter)
UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 Date of Report (Date of earliest event
More informationExecutive Committee and Officers ( )
Gifted and Talented International V o l u m e 2 4, N u m b e r 2, D e c e m b e r, 2 0 0 9. G i f t e d a n d T a l e n t e d I n t e r n a t i o n a2 l 4 ( 2), D e c e m b e r, 2 0 0 9. 1 T h e W o r
More informationTECHNICAL MANUAL OPTIMA PT/ST/VS
TECHNICAL MANUAL OPTIMA PT/ST/VS Page 2 NT1789 Rév.A0 TABLE OF CHANGES The information contained in this document only concerns : OPTIMA PT/ST/VS type, MCM 440 PT/OT type, MCM550 ST type. Technical Manual
More informationMatrix-assisted laser desorption ionization/time of flight (MALDI/TOF) mass spectra were obtained on
MALDI-TOF Mass Spectroscopy Matrix-assisted laser desorption ionization/time of flight (MALDI/TOF) mass spectra were obtained on Ultraflextreme (Bruker Daltonics, Germany) mass spectrometer using sinapinic
More informationVlaamse Overheid Departement Mobiliteit en Openbare Werken
Vlaamse Overheid Departement Mobiliteit en Openbare Werken Waterbouwkundig Laboratorium Langdurige metingen Deurganckdok: Opvolging en analyse aanslibbing Bestek 16EB/05/04 Colofon Ph o to c o ve r s h
More informationFor the rapid, sensitive and accurate detection of active Caspase 9 in living cells
ab65615 Caspase 9 (active) FITC Staining Kit Instructions for Use For the rapid, sensitive and accurate detection of active Caspase 9 in living cells This product is for research use only and is not intended
More informationSpeed of light c = m/s. x n e a x d x = 1. 2 n+1 a n π a. He Li Ne Na Ar K Ni 58.
Physical Chemistry II Test Name: KEY CHEM 464 Spring 18 Chapters 7-11 Average = 1. / 16 6 questions worth a total of 16 points Planck's constant h = 6.63 1-34 J s Speed of light c = 3. 1 8 m/s ħ = h π
More informationMonitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening
APPLICATION NOTE ArrayScan High Content Platform Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening Suk J. Hong and Richik N. Ghosh
More informationAdditional Materials Required (NOT PROVIDED): 200 µl; anti-ifn-ω monoclonal (murine) antibody Streptavidin-HRP* 150 µl
Protocol VeriKine TM Human Interferon Omega ELISA Kit Catalog No: 41395 Lot No: Expiration: Assay Range: 4.7-300 pg/ml Store all components at 2 8 C Please review the protocol in its entirety prior to
More informationRANK. Alternative names. Discovery. Structure. William J. Boyle* SUMMARY BACKGROUND
RANK William J. Boyle* Department of Cell Biology, Amgen, Inc., One Amgen Center Drive, Thousand Oaks, CA 91320-1799, USA * corresponding author tel: 805-447-4304, fax: 805-447-1982, e-mail: bboyle@amgen.com
More informationB ooks Expans ion on S ciencedirect: 2007:
B ooks Expans ion on S ciencedirect: 2007: 1 INFORUM, 22-24 May, Prague Piotr Golkiewicz Account Manager Elsevier B.V. Email: p.golkiewicz@elsevier.com Mobile: +48 695 30 60 17 2 Pres entation Overview
More informationMillifluidic culture improves human midbrain organoid vitality and differentiation
Millifluidic culture improves human midbrain organoid vitality and differentiation Emanuel Berger University of Luxembourg 3D midbrain organoids for modelling Parkinson s disease Healthy control Midbrain
More informationAssessment of toxicological properties and establishment of risk profiles - genotoxic properties of selected spice compounds
Assessment of toxicological properties and establishment of risk profiles - genotoxic properties of selected spice compounds Frankfurt/Main, September 25 th 212 V.J. Koller 1, V. Auwärter 2, G. Zlabinger
More informationSupplementary Tables and Figures
Supplementary Tables Supplementary Tables and Figures Supplementary Table 1: Tumor types and samples analyzed. Supplementary Table 2: Genes analyzed here. Supplementary Table 3: Statistically significant
More informationTexas Student Assessment Program. Student Data File Format for Student Registration and Precoding
Texas Student Assessment Program 2018 Student Data File Format for Student Registration and Precoding 2 2018 Student Data File Format for Student Registration and Precoding Submission Schedule of Student
More informationDesign of Microarray Experiments. Xiangqin Cui
Design of Microarray Experiments Xiangqin Cui Experimental design Experimental design: is a term used about efficient methods for planning the collection of data, in order to obtain the maximum amount
More informationInformation System Desig
n IT60105 Lecture 7 Unified Modeling Language Lecture #07 Unified Modeling Language Introduction to UML Applications of UML UML Definition Learning UML Things in UML Structural Things Behavioral Things
More informationUse precise language and domain-specific vocabulary to inform about or explain the topic. CCSS.ELA-LITERACY.WHST D
Lesson eight What are characteristics of chemical reactions? Science Constructing Explanations, Engaging in Argument and Obtaining, Evaluating, and Communicating Information ENGLISH LANGUAGE ARTS Reading
More informationUNITED STATES SECURITIES AND EXCHANGE COMMISSION FORM 8-K. Farmer Bros. Co.
UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 8-K CURRENT REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 Date of Report (Date of earliest event
More informationLactate Dehydrogenase Assay Kit
Lactate Dehydrogenase Assay Kit Catalog Number KA0878 500 assays Version: 08 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More information