The Most Comprehensive Source of Recombinant Human Proteins

Transcription

1 Th Ms Cmphsv Su f Rmb Hum Ps M ssys, b ssys 17,000 Ov-xpssd yss f hum ps 6,000 Pufd hum ps pdud HEK293 s 6,000 Hvy bd ps s Mss Sp sdds Rvs-phs p y (RPPA)

2 17,000 Hum Ps s Ov-xpss Lyss Fu gh hum ps f fu suds d bdy vd FEATURES 17,000 v-xpss yss f fu gh hum ps Expssd s C-m My-DDK* g f sy d d s I RIPA buff wh SDS bs psv p vy APPLICATIONS Psv s Ws, mmupp,. Sdds ELISA d h ssys P fu sudy Rvs phs p ys App d #1: Abdy vd Vd A ***** Vd B *** Vd C * Vd D **** Vd E kd g 45g 22g 4.5g 2.2g 221g 45g 22g 4.5g 2.2g 221g 45g 22g 4.5g 2.2g 221g 45g 22g 4.5g 2.2g 221g 45g 22g 4.5g 2.2g Fv mm bds gs hum P53 w vud Ws b xpms wh P53 v-xpss ys. P53 p v ys ws p-dmd usg pufd GST-My-DDK sdd. Lys ws sy dud bf SDS-PAGE d mmubg. Abdy quy d s g s bsd P53 p d v. Th Hum P As pj dpd OG v-xpss yss d sgfy sd u py bdy Ws b suss. W vy hppy wh h sus. Pf Mhs Uh, Ry Isu f Thgy (KTH), Skhm, Swd

3 App d #2: P vy ssy GATA4 (LY419558) DNA-Bdg Avy f GATA4 Ov-Expss Lys (LY419558) Fus Us 4,000 3,500 3,000 2,500 2,000 1,500 GATA4 Ov-xpss Lys C Lys 1, DNA-bdg vy f GATA4 ws msud OG's v-xpss ys LY d ys. Th ms f h ys ws sd wh sp f bdg ssy uzg GATA4-spf DNA squs. Th hgh v f vy bsvd h v-xpss ys mpd h ys dmss h h xpssd GATA4 s bgy v h ys. App d #3: P ys md fm v-xpss yss Egd mg E-Cdh1 A-DDK (Rbb) A-E-Cdh1 (mab) Usg OG g my hp hgy dd bds h w gd by wh mmuz OG s hgh dsy p y md wh m h 10,000 uqu v-xpss yss, pd dup, wh s. Suh p ys b usd bdy spfy vd, p-p, d f u-bdy pfg.

4 6,000 Pufd Hum Ps Expssd HEK293 Cs FEATURES Pdud wh TuORF DNA s 6,000 fu gh hum ps Expssd HEK293 s Opm psv f p suu, ps-s mdfs d fus Lg s pdu vb COMPARISON OF DIFFERENT EXPRESSION SYSTEMS Mmm Ys Is s E. P fdg d puf Opm P Lw P Ps-s pssg Ys Lw Lw N Auhy & Bvy Nv d v P P Vy p APPLICATIONS MRI1 GSTK1 SERPINA1 PCBD1 MAPK1 FCN1 MNE1 PRKCD EPHB4 TARDBP Nv gs f pmzd bdy pdu Psv s bdy bsd mmussys, suh s ELISA P-p I v bhm ssys d -bsd fu ssys Buff d Sg: 10% gy, 100 mm gy, 25mM Ts-HC, ph 7.3. S -80 C. Puf: Th v-xpssd p ws pufd usg -DDK ffy um Tgs: C-m My-DDK C: > 50 ug/m Puy: > 80% s dmd by SDS-PAGE Edx: < 0.1EU/ug f p DDK-g s h sm s FLAG g. Fg s gsd dmk f Sgm-Adh.

5 Ap p d #1 : P u f dp b v y( MT OR) Ap p d #2: Hu m p d u dp m p h E. d v dp

6 Hvy-bd Fu-gh P s MS Sdds Au quf f hum p bmks Quv mss spmy, spy MRM-MS d SRM-MS, pys sgf p bmk dsvy d vd. OG ffs h sv g hvy sp bd 6,000 ps s MS sdds. Spkg h y sg f smp pss f u quf Idfy h bs SRM d MRM ss hugh xpm d Auh ps-s mdfs by usg s Hgh d ssy h syh ppd sdd Hvy Isp Lbd Fu Lgh P Sdd A B Su f Quv Mss Spmy P Ex F Typs Dgs OG TuMS-Sd Smp C RP-HPLC AQUA Ppd Sd MRM Sg f g p Q1 Q2 Q3 Isy Tm MRM Sg f hvy ppd Ppd S Fgm Fgm S Isy Tm OG d h Isu f Sysms Bgy wk gh pyp PpdAs d SRM/MRM mss spmy sdd dbs f 5,000 hum ps, gy quv p bmk dsvy.

7 1, , , , , , C Tssu Lys Ays (Rvs-Phs P Ays) Lg s, quv s f p bmk sh PS C Lys Ays hgh-dsy vs-phs p ys (RPPA, RPA) ssmbd fm 432 d m ssu p yss. Wh p-spf bds, PS C Lys Ays b quv df d vd f p xpss pfs d ps-s mdfs ssd wh 11 yps f. UTILITIES Hgh dsy ys f quv yss f p xpss d ps-s mdfs Idfy ssd p xpss wh yss fm 432 ssus vg 11 yps f Esy fw ps f hd m fum ssys SIMPLE PROTOCOL If yu d Ws b, yu d RPPA sy. Sp 1: Pmy bdy Fgu 2 Smp yss f dff xpss dx (smp du dvdd by md f h ssu m smps) f fu dff ps psd h mp fm. Sp 2: Sdy bdy Sp 3: Sg mpf (.g., TSA sysm) Sp 4: Rd d yz sus Fgu 1 A TP53 vy wh RPPAs sd wh m TSA hm p. PS C Lys Ay Cum 1 ERBB2, Cum 2 BIRC5, Cum 3 TP53, Cum 4, Phsph My C Nm C C Bs Nm Bs C < > Kdy C Kdy Nm Lug C Lug Nm Lv C Lv Nm Lymphm C Lymphm Nm Mm C Mm Nm Ov C Ovy Nm Ps C Ps Nm Ps C P Nm Smh C Smh Nm

8 OG, Yu P G Rsh d Byd KEY TECHNOLOGIES AND PRODUCTS Fu-gh DNA s, ORF s xpss-dy vs G syhss: y g, y v, y v RNA sh gs: shrna, srna, d mrna fu d d SYBR G qpcr ssys f mrna d mrna d, pm ps Rmb hum ps d v-xpss yss TuMAB m bds Lumx mupx mmussys C ssu bpsy, TMA, RPPA, d TssuS qpcr ys UMAB vdd IHC bds OG USA 9620 Md C Dv, Su 200 Rkv, MD OG G Syhss Bu H Bh h Avu SE #100 Bh, WA USA OG CHINA HuLgS I S & Thgy Idusy Pk Bud 11, N.99, Khug Fuh S, D4 Bjg, CHINA