Theory module: 11(MCQ) CENTRIFUGATION
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1 Theory module: 11(MCQ) 1. A primary objective of cell fractionation is A. to crack the cell wall so the cytoplasmic contents can be released. B. to identify the enzymes outside the organelles. C. to view the structure of cell membranes. D. to separate the major organelles so their particular functions can be determined. E. to determine the size of various organelles. 2. It is widely used to separate and purify biological particles in a liquid medium under applied centrifugal force. A. Centrifugation B. Microscope C. ph meter 3. In this type of rotors, the sample tubes are loaded into individual buckets that hang vertically while the rotor is at rest. When the rotor begins to rotate the buckets swing out to a horizontal position A. swinging-bucket B. fixed-angle C. vertical 4. In this type of rotors, the sample tubes are held fixed at the angle of the rotor cavity. When the rotor begins to rotate, the solution in the tubes reorients A. swinging-bucket B. fixed-angle C. vertical 5. This type of rotor is not suitable for pelleting applications but is most efficient for isopycnic (density) separations due to the short path length. A. swinging-bucket B. fixed-angle C. vertical CHRIST COLLEGE, RAJKOT, GUJARAT. Page 1
2 6. When designing a centrifugation protocol, following factors could play important role. A. The more massive a biological particle is, the faster it moves in a centrifugal field B. The denser the biological buffer system is, the slower the particle will move in a centrifugal field; C. The greater the frictional coefficient is, the slower a particle will move; D. All of the above 7. RPM stands for A. Round per minutes B. Right per minutes C. Revolution per minutes 8. In equation, G= 2 r where equals to A. Angular velocity B. radial distance from the axis of rotation C. Centrifugal force 9. Separation of analyte is based on A. size, B. shape C. density. D. All of the above 10. This technique is used to separate molecule with same size of particles (M) but different shapes A. Zones of equal density centrifugation B. preparative ultracentrifuges C. None of the above D. All of the above CHRIST COLLEGE, RAJKOT, GUJARAT. Page 2
3 Theory module: 11(Assignment) Answer brifely 1. Write principle of sedimentation and centrifugation? 2. Derive the equation to establish relationship between RCF and RPM? 3. How frictional force influences centrifugation process? 4. How to safeguard centrifuge? 5. Write the applications of centrifugation? 6. Define following terms : Centrifugation, Sedimentation velocity, fricitional coefficient, RFC, RPM CHRIST COLLEGE, RAJKOT, GUJARAT. Page 3
4 Theory module: 11 (References) Outlines of Biochemistry by E.E Conn and P.K.Stump Practical of Biochemistry by Wilson & Walker Instrumental Methods of Analysis 7th Edition by M.H. Willard CHRIST COLLEGE, RAJKOT, GUJARAT. Page 4
5 Theory module: 11 (Edutainment) CHRIST COLLEGE, RAJKOT, GUJARAT. Page 5
6 Across 58. centrifugation, is used to separate particles from a liquid medium or to separate particles of different masses into separate fractions of the supernatant. 39. G=w x w x r where w= 81. Type of Centrifuge Rotors : Angle Rotor 94. Unit of angular velocity of rotor ( /sec) 24. In gradient centrifugation, a centrifuge tube is filled with a CsCl (cesium chloride) or sucrose solution. The solution is at different concentrations, denser towards the bottom. 7. rpm = per minute Down 14. Device used in a variety of scientific and technical applications which spin carrier vessels (centrifuge tubes) at high rotation speeds. 64. Each centrifuge has a special graph called a, or a table which relates rotation rate (rpm) to centrifugal force (xg) for each size of rotor it accepts. 18. centrifugal force (RCF) 62. In centrifuge, the rotating unit is called 76. In centrifugation the separation of analyte is based on, shape and density. 86. G=w x w x r where r= distance from the axis of rotation (in cm) 4. density centrifugation uses the concept of a buoyant force to separate the molecules 3. It is widely used to separate and purify biological particles in a liquid medium under applied centrifugal force. 1. matter that settles to the bottom of a liquid CHRIST COLLEGE, RAJKOT, GUJARAT. Page 6
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