Vet Photometer DP 700 Operating Manual. Version V5.9
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1 1 Vet Photometer DP 700 Operating Manual Version V5.9
2 2 Dear customer, Thank you for selecting Diaglobal s Vet Photometer and for the confidence you have placed in us. The Vet Photometer belongs to a new generation of small mobile instruments which are being developed by Diaglobal GmbH and are specially designed for on-site analysis. An automatic operational device check has been additionally integrated with the actual software version V5.4. The Vet Photometer can be used to determine four clinical-chemical parameters from serum/plasma, blood: NEFA (free fatty acids), Lactate, Calcium and Magnesium. The kits and accessories which are required to carry out the tests and measurements are also available from Diaglobal GmbH. All the best for your work with the new Vet Photometer! Yours Diaglobal GmbH
3 3 Table of contents Page 1. General information regarding the Photometer 4 2. Installation 5 3. Description of the instrument Power supply Mains power operation Network-independent operation Measuring system 6 4. Service Calibration Maintenance Disturbances Disposal 7 5. Required reagents and laboratory accessories Reagents / parameter list Control materials Disposables and accessories 8 6. Measuring process Multipoint measurement and recognition of the end point End point measurement taking standard 8 7. Measurement Switching the instrument on Self-testing when switching on Test selection Exit the program / Switching the instrument off Integrated operational device checks 9 8. Technical data General guidelines and notes Appendix: Step-by-step measurement 12ff
4 4 1. General information regarding the Photometer Device name: Vet Photometer Model: DP 700 Features: In-vitro diagnostics, measuring instrument for the determination of NEFA (free fatty acids), Calcium and Magnesium from serum/plasma and Lactate from plasma/blood The Vet Photometer fulfils the basic requirements of Low Voltage Directive 2006/95/EG. The conformity of the instrument with Directive 2006/95/EG is confirmed by the use of the CE marking. The Vet Photometer was specially developed for analyses in veterinary medicine, however it is universally applicable. Manufacturer: Diaglobal GmbH Innovationspark Wuhlheide Köpenicker Str. 325 D Berlin Tel: Fax: info@diaglobal.de
5 5 2. Installation Please observe the following instructions for use: Insert the rechargeable battery if the instrument is to be used networkindependent or connect the photometer to the power supply unit. Press the key </> (Fig. 1) to activate the internal instrument check which is automatically carried out by the instrument. Following this, the instrument is immediately ready for measuring. 3. Description of the instrument Cuvette shaft Display Function keys Fig. 1
6 Power supply The Vet Photometer can be operated as desired using a power supply, a (9V block) battery or (model 6F22 or PP3) rechargeable battery Mains power operation The power supply connector plug is connected to the instrument s socket. The Vet Photometer is provided with a power supply unit (6-9V=) for operation at 230 V/50 Hz Network-independent operation Insertion of the rechargeable battery or the normal battery: Unscrew the knurled screws on the bottom side and remove the cap. Connect the rechargeable battery or the normal battery using the push-button contact and insert it. Place the cap back on top and fasten the knurled screws. The rechargeable battery cannot be charged whilst it is installed. A separate battery charger is required for this purpose. Please note: The Vet Photometer can be operated using a power supply and it is not necessary to remove the rechargeable battery or the normal battery for this purpose. 3.2 Measuring system The optical section is shown in Fig. 2. Focus Cuvette Fig. 2 The light emitted by an LED is first selected by an interference filter IF (HBW ~ 5 nm) in its spectral ranges (520 nm) and then guided concentrated to the cuvette in the shaft. After it has passed through the cuvette, a broadband photo sensor converts the light falling onto its sensor surface to current, proportional to its intensity.
7 4. Service Calibration The instrument is works calibrated when supplied - it is not necessary for the customer to calibrate it. 4.2 Maintenance After the expiration of warranty we recommend regular yearly maintenance of the instrument. 4.3 Disturbances If any disturbances or problems occur, simply call us. We can answer most questions on the telephone. Faulty instruments must be sent to our Berlin address. We can lend our customers an instrument whilst theirs is being repaired. 4.4 Disposal We take back instruments free of charge which are no longer needed or cannot be repaired and dispose of them. 5. Required reagents and laboratory accessories 5.1 Reagents / parameter list The following tests can be measured by the Vet Photometer: Parameter Sample material Blood Serum Plasma Tests/Pckg. Art. No. NEFA NEFA 013 Lactate LAC 142 Calcium CA 015 Magnesium MG Control materials Art. No. Description Contents LAC QS Lactate Control Set 2.00 / 4.00 / 10.0 mmol/l 3 x 4 ml CA QS Calcium-/Magnesium-Control 3 x 1.5 ml NEFA QS NEFA Control 3 x 1.0 ml
8 5.3 Disposables and accessories 8 Art. No. Description Contents LH 004 Capillaries 10 µl, with ringmark (Micropipettes) 250 LH 006 Cuvette rack 1 LH 007 Micropipettor (pipetting aid) 1 LH 009 Cellulose swabs 500 LH 010 Cellulose swab box 1 LH 011 Alcohol pads, non-sterile 100 LH 012 Latex gloves, size 6-7 small or 7-8 medium 100 LH 053 Pipette tips ml, clear 200 LH 054 Pipette tips µl, yellow 500 LH 055 Pipette tips µl, blue 500 LH 503 Pipette fix 1500 µl 1 LH 504 Pipette variabel ml 1 LH 550 Pipette fix 50 µl 1 All reagent kits, control materials and other materials can be stored and transported together with the Vet Photometer in a practical case. 6. Measuring process 6.1 Multipoint measurement, taking the sample s blank point and recognition of the end point into account Parameter: Lactate (LAC 142) After measurement of the sample blank value (= measurement 1), the colour reaction is started in the cuvette. The reaction process is monitored by the instrument (= measurement 2). The measuring procedure is stopped as soon as the end point is reached. The time needed to reach the end point is temperature-dependent. It is normally 2-6 minutes for the lactate test. If temperatures are close to freezing point, measuring times of up to 20 minutes may result, dependent on the parameters. Both single measurements and series measurements can be selected. If single measurements are made, the samples are measured one after another. For series measurements, all A1 values are measured first. Calculation of concentration = Absorbance Difference x Factor Calculation is made using a complex special program by the photometer 6.2 End point measurement taking standard Parameter: NEFA (free fatty acids), Calcium and Magnesium Measurement of absorbance after reaching the end point in relation to the reagent blank value and taking standard. Calculation: Concentration = Factor x (EAnalysis - EBlank) / (EStandard - EBlank)
9 7. Measurement Switching the instrument on Press the < / > key 7.2 Self-testing when switching on A self-testing of the digital and analogue circuit occurs when switching on the instrument. The operational device check proceeds automatically after the switching-on. It lasts ca. 5 seconds, after this the device is ready for measuring. Note: In case during the testing it should emerge that one of the device functions does not satisfy the required settings, the following display appears: <SERVICE> In this case please switch off the instrument. Please call the Service of Diaglobal GmbH (tel / 30 / ) or contact your speciality retailer. 7.3 Test selection Press the < / > key Select the desired method using from the menu using the right or left arrow key: NEFA - LAC - CA - MG - ABS520 Press the right arrow key to mark the respective next test and the left arrow key to return to the previous test. The test currently selected is shown in the upper right-hand corner of the display. Confirm test selection by pressing the < / > key 7.4 Switching the instrument off To switch the instrument off, press the keys together. 7.5 Integrated operational device checks Self-testing when switching on The testing of the device s digital and analogue circuit occurs automatically already when switching on the instrument. Please see chapter 8, point 2. Differential measurements All measurements are based on differential measurements, i.e. after having chosen the desired test the instrument requests a reference measurement with a blank value cuvette. Thus, a reference basis to the measured value is established so that minor deviations may be compensated.
10 10 Measurement range controls The measurement ranges of all measurement results indicated in the display become verified by an integrated measurement range control. An error display appears when exceeding the measurement range. All measurement ranges, which are separately defined for each parameter, are documented in the respective package inserts as well as in the operating manual, chapter 9, technical data. Plausibility controls When performing multiple point measurements the extinction, which was measured first, represents the reference basis. The programme tests the individual measured values for plausibility. In case particular requirements (e.g. E2 > E1 for rising reactions) are not met, an error message is displayed.
11 11 8. Technical data Storage temperature: Operating temperature: Dimensions: Weight: Measuring principle: Projection: Spectroscopic apparatus: Measuring wavelengths: Spectral half-width value: Extraneous light influence: - 20 C...70 C 0 C...40 C 200 x 100 x 50 mm 450 g Absorption metering with a single-beam photometer (fig. 2), choppered operation LED Interference filter 520 nm ~ 5 nm Negligible Interface: V24 (9600, 8, n, 2) Power supply: Warm-up time: 9V battery or rechargeable battery block 6F22 or PP3 optional plug-in power pack 0 min Interference suppression: According to DIN VDE 0871 and DIN VDE 0875 Electromagnetic Compatibility: According to EN Measuring ranges: NEFA mmol/l LAC CA MG mmol/l mmol/l mmol/l ABS 520nm E = Inaccuracy: < 0.5 % at E = Relative photometric short-time standard deviation: < 0.1 %
12 12 9. General Guidelines and Notes EC Directives 1. Low Voltage Directive 2006/95/EG EN / ISO standards 2. EN ISO 9001:1994, Qualitäty Management Systems, Model for quality assurance in design, development, production, installation and servicing 3. EN , Safety requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements 4. EN , Electrical equipment for measurement, control and laboratory use - EMC requirements - Part 1: General requirements Note on electromagnetical compatibility a) This photometer meets the requirements of the IEC series of standards regarding electromagnetic radiation and interference immunity. b) Do not use this unit near to sources of intense electromagnetic radiation as these may disturb its correct functioning. During the measurement, a distance of at least 1 m should be kept between an operative (turned on) mobile telephone and this photometer. Note on the unit's internal quality control This unit checks its functionality when it is turned on. Moreover, it performs electronically controlled individual tests during the measurement causing an error message when defined conditions are not met. 10. Appendix: Step-by-step measurement Please refer to the illustrations of the following pages.
13 Step by step instructions Device manual 1. Switch on: 2. Select test: Press / Press arrow key until required test appears Wait for device check and confirm with / 3. Confirm required test: 4. Switch on: Press / Press both arrow keys at the same time Note: If SERVICE appears after the device check, the device has a fault. In this case, please contact our customer service at +49 (0) Diaglobal GmbH Köpenicker Straße Berlin +49 (0) info@diaglobal.de
14 Step by step instructions NEFA (free fatty acids) Example: 2 samples Additionally required: Free fatty acids standard (NEFA ST), Diaglobal-round cuvettes (LH 075) 1. According to instruction prepare the reagent mixtures Thereafter, pipette 1000 µl of the bottle R1a in each cuvette 2. Pipette samples: cuvette 1: nothing (= blank) cuvette 2: 50 µl standard cuvette 3: 50 µl sample 1 cuvette 4: 50 µl sample 2 Close cuvettes, mix and leave for 10 minutes 3. After 10 minutes of waiting time pipette 500 µl R2a in each cuvette Close and mix again and leave again at room temperature for 10 minutes Insert NEFA Insert standard NEFA NEFA Standard Abs blank 4. Switch on the photometer with / Wait for device check, confirm with / Select NEFA, confirm with / 5. Blank adjustment: Insert cuvette 1 (= blank) into photometer, blank is stored in memory Remove after signal tone 6. Insert cuvette 2 with standard into photometer Photometer shows Absorption of cuvette with standard Hint: If necessary, note this value for documentation purposes. Sample 01 insert NEFA NEFA Sample mmol/l 7. Remove cuvette 2 with standard 8. Insert cuvette 3 (sample 1) and thereafter cuvette 4 (sample 2) into photometer Read results successively In regard to serial measurement After blank setting any number of additional samples can be measured Hint: To verify the measured values, we recommend carrying a control: NEFAcontrol (Art. No. NEFA QS) Diaglobal GmbH Köpenicker Straße Berlin +49 (0) info@diaglobal.de
15 Step by step instructions CA 015 / MG 013 Example: 2 samples Additionally Required: Calcium-Magnesium-Standard (CA ST), Diaglobal-round cuvettes (LH 075) CA Insert blank 1. According to the instructions, pipette the prescribed amounts buffer and color solution into cuvettes Thereafter, pipette the standard and the two samples (fig. 2) 2. Pipette into cuvettes, mix well: cuvette 1: nothing (= blank) cuvette 2: standard cuvette 3: sample 1 cuvette 4: sample 2 CA 015: 50 µl / without waiting time MG 013: 10 µl / wait 5 minutes 3. Switch on the photometer with / Wait for device check, confirm with / Select the required test, confirm with / Insert standard CA Standard Abs CA Sample 01 insert CA 4. Blank adjustment: Insert cuvette 1 (= blank) into photometer, blank is stored in memory Remove after signal tone 5. Insert cuvette 2 with standard into photometer Photometer shows Absorption of cuvette with standard Hint: If necessary, note this value for documentation purposes. 6. Remove cuvette 2 with standard CA Sample mmol/l 7. Insert cuvette 3 (sample 1) and thereafter cuvette 4 (sample 2) into photometer Read results successively In regard to serial measurement After blank setting any number of additional samples can be measured In regard to quality control To verify the measured values, we recommend carrying a control: Calcium Magnesium Control (Art. No. CA QS) Diaglobal GmbH Köpenicker Straße Berlin +49 (0) info@diaglobal.de
16 Step by step instructions LAC 142 / LAC 342 / GLU 142 / CHO 142 / TRI 142 Single measurement 1. Insert capillary with sample into cuvette 2. Wash out sample with micropipetter 3. Screw on cap Turn cuvette upside down several times M1 LAC M1 LAC Sample 01 insert Sample 02 insert or 4. Switch on photometer with / Wait for device check, confirm with / Select the required test, confirm with / 5. Blank adjustment: Insert cuvette with sample (fig. 3) into photometer, blank is stored in memory Remove after signal tone 6. Replace screw cap against starter cap M2 LAC Sample 01 00:30 Measuring 7. Turn cuvette upside down several times 8. First press / Thereafter insert cuvette in photometer 9. Timing appears Wait for result Diaglobal GmbH Köpenicker Straße Berlin +49 (0) info@diaglobal.de
17 Step by step instructions LAC 142 / CHO 142 / TRI 142 Serial measurement M1 Sample 01 insert LAC 1. Wash out samples from capillaries into cuvettes with micropipetter 2. Screw on cap Turn cuvette upside down several times 3. Switch on photometer with / Wait for device check, confirm with / Select the required test, confirm with / M1 Sample 19 insert or LAC 4. Blank adjustment: Insert cuvette with samples (fig. 2) into photometer, blank is stored in memory Ensure correct order of samples! 5. After blank adjustment of the last cuvette replace all screw caps against starter caps 6. Turn cuvettes upside down several times at the same time M2 LAC Sample 01 00:30 Measuring M2 LAC Sample mmol/l 7. First press / Thereafter insert cuvette 1 in photometer Timing appears Wait for result 8. Read the measured value of cuvette 1, remove cuvette Insert cuvette 2, read the measuring value, remove cuvette etc. 9. Repeat the process until the measured value of the last cuvette is displayed Pay attention to correct assignment and sequence of samples! Diaglobal GmbH Köpenicker Straße Berlin +49 (0) info@diaglobal.de
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