INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES
|
|
- Melanie Powers
- 5 years ago
- Views:
Transcription
1 International Journal of Universal Pharmacy and Bio Sciences 2(4): July-August 2013 ITERATIAL JURAL F UIVERSAL PHARMACY AD BI SCIECES IMPACT FACTR 1.89*** ICV 3.00*** Pharmaceutical Sciences Research Article!!! Received: ; Accepted: METHD DEVEPMET AD VALIDATI F PIPERAQUIE PHSPHATE AD ARTERLAE MALEATE I CMBIED DSAGE FRM BY RP-HPLC TECHIQUE Swathi Induru * 1, aira ayeem 2, Sandeep kolluru 1 1 Dept of Pharmaceutical Analysis, 2 Dept of Pharmaceutical Chemistry Krupanidhi College of Pharmacy, Bangalore KEYWRDS: Piperaquine Phosphate, Arterolane Maleate, RP- HPLC, Combined Dosage Form. For Correspondence: Swathi Induru * Address: Department of Pharmaceutical Analysis, krupanidhi college of Pharmacy, #12/1,Chikkabellandur village, Carmalaram post, Varthur, Hobli, Bangalore swathi.induru@gmail.co m ABSTRACT A reverse phase HPLC method has been developed for the simultaneous determination of Piperaquine phosphate (PQP) and Arterolane maleate (AM) in combined dosage form by using C 18 Thermo Hypersil BDS, (10 X 4.6 mm X 5.0µ) column and the mobile phase was prepared by mixing methanol: potassium dihydrogen phosphate buffer (ph- 2.3) in the ratio of (50:50). Retention times of PQP and AM were 1.13 min and 1.83 min respectively. This method shows to be linear (r 2 >0.99), precise (RSD<2%), accurate recovery of (98-102)% of PQP and (98-102)% of AM. specific and robust. 424
2 ITRDUCTI: Piperaquine phosphate is chemically known as 7-chloro-4-[4-[3-[4-(7-chloroquinolin-4- yl)piperazin-1-yl]propyl] piperazin-1-yl]quinoline; phosphoric acid [1]. Arterolane maleate is chemically known as [(-(2-amino-2-methylpropyl)-2-cis-dispiro(adamantane-2,3'- [1,2,4]trioxolane-5',1"- cyclohexan)-4"-yl]acetamide: maleate [2]. PQP and AM acts as Anti- Malarial drugs. PQP interferes with the degradation of haemoglobin by parasitic lysosomes, helps in damaging of plasmodial membranes. AM causes lipid peroxidation, damages endoplasmic reticulum, inhibits protein synthesis and ultimately results in lysis of the parasite [3]. Highly Sensitive, Selective, HPLC method will be very useful for the estimation of PQP and AM in combined dosage formulations. Literature survey reveals need for simultaneous estimation of drugs. Few methods were reported by HPLC [4-7], LC-MS [8-11], Capillary Zone electro phoresis [12]. The purpose of this study was to develop sensitive, simple, precise, accurate method for Simultaneous estimation of PQP and AM in bulk and combined dosage form. Fig o.1 Chemical Structure of Piperaquine phosphate and Arterolane maleate Cl CH 3 H 2 H 3 C H H P H H H H MATERIALS AD METHDS Apparatus: Cl Separation and estimation was carried out using HPLC (Waters-2469 with PDA detector), column used in experiment was C 18 Thermo Hypersil DS, 4.5µ (250 X 5.4 mm) analytical balance used was LABIDIA, Digital ph meter LABIDIA-PHA,. The mobile phase was prepared by mixing Methanol: potassium dihydrogen phosphate Buffer (ph-2.3) in the ratio of (50:50) was CH 4 filtered and degassed. Injection volume is 10µL and the detection was at 240nm. Reagents and solutions: Pure sample of piperaquine phosphate and arterolane maleate and other reagents such as methanol, double distilled water of HPLC grade and potassium dihydrogen phosphate. 425
3 Preparation of standard drug solutions Weighed accurately about 15.0 mg of the PQP and 75.0 mg AM pure drug sample and transferred into 100ml dry standard volumetric flask to this added 20ml of water and sonicated 10 minutes and make up the volume with water. From the above solution transferred 5 ml to 5o ml volumetric flask and diluted with water. Preparation of sample drug solution Randomly selected 10 tablets and determined the average weight of tablets and crushed into fine powder. Weigh and transferred tablet powder equivalent to 750 mg of PQP into a 1000 ml of volumetric flask, to this added 250ml of water and sonicated for 10 minutes and make up the volume with water. Further diluted 5ml of this solution to 50 ml with water and mix well. Filtered the solution. Marketed Formulation: Table o.1 Details of marketed Formulation Brand name Content Mfg. Company Synriam Preparation of mobile phase PQP & AM (750mg & 150mg Respectively) Ranbaxy The mobile phase was prepared by mixing methanol: potassium dihydrogen phosphate (ph-2.3) in the ratio of (50:50) and was filtered and degassed. Selection of mobile phase for method ptimization and experimental condition: Several trial had been taken for the proper optimization of RP HPLC method by changing different mobile phase with different ratio. And finally the mobile phase for optimised condition methanol: potassium dihydrogen phosphate (ph-2.3) in the ratio of (50:50)was selected and chromatogram was shown in Fig no.2. Fig : 2 Chromatogram of PQP and AM for ptimised method 426
4 Table: 2 Chromatographic conditions for the optimized method Parameters Description Column C 18 Thermo Hypersil BDS, (10 X 4.6 mm X 5.0µ) Mobile phase Methanol: Buffer (50:50) Injection volume 10 µl Flow rate 1.2 ml/min Detector Wavelength 240 nm Column Temperature 40 C Sampler Temperature 25 C Run Time 4 min Assay: Assay of marketed tablet formulation containing PQP (750mg) and AM (150mg) was performed by preparing the sample solutions as described earlier in the preparation of the sample. The assay of the commercial sample was calculated by comparing the areas of standard and sample peaks. The assay of marketed formulation synriam was found to be within the limit; the chromatogram is shown in Fig 3. Fig:3 Chromatogram for the Assay of marketed Formulation This optimized method was validated in terms of linearity, accuracy, precision, specificity, as per ICH guidelines [13]. Linearity: The linearity of calibration curves (peak area ratio V s concentration) in pure solution was checked over the concentration ranges of about % (Assay concentration in mcg/ml) for PQP and AM. The total eluting time was less than 4.0 min. The calibration curves were linear in the studied range. and equations of the regression analysis were obtained: y = 80651x, R 2 =0.999 for PQP and y = 14057x, R 2 =1.0 for AM. Accuracy: Accuracy of the method was determined by recovery experiments at spiked levels of 50%, 100%, 150%. The recovery studies were carried out three times, the percentage recovery and percentage relative standard deviation were calculated. 427
5 Precision: The precision of the analytical method was studied by analysis of multiple sampling of homogeneous sample. Specificity: A solution containing a mixture of tablet was prepared using sample preparation procedure and injected into the system, to evaluate possible interfering peaks. RESULTS AD DISCUSSI Validation: Method Precision Table 3. Method Precision Results for Piperaquine phosphate Test no. Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Avg. wt (mg) Wt. taken (mg) Area (Inj.1) Area (Inj. 2) Avg. Area % RSD Assay (mg/tab) Assay (%) Average Assay In mg = In % = Table 4. Method Precision Results for Arterolane maleate Test no. Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Avg. wt. taken (mg) Wt. taken (mg) Area (Inj.1) Area (Inj. 2) Avg. Area % RSD Assay (mg/tab) Assay (%) Average Assay In mg = In % = System suitability tests were carried out on freshly prepared standard solution and all the parameters are within limit. Results were shown in table o.5. TABLE : 5 System suitability Data Parameters PQP (± %RSD) AM (± %RSD) Retention Time 1.13± ±0.14 Theoretical plate 9348± ±0.34 Tailing Factor 1.14± ±0.23 ± %RSD = Percentage Relative Standard Deviation. 428
6 The method was linear in the concentration range of µg/ml for PQP and µg/ml for AM, with a correlation coefficient of for PQP and 1.0 for AM. Fig: 4 Linearity plot of PQP and AM The study of accuracy of the developed method has been done. The recovery was found in the range of 99.3%for PQP and 99.7%for AM shown in Table.6, indicating the accuracy of method and the % RSD of PQP and AM is 0.14 and 0.23 respectively. Concentration of Spiked level % TABLE: 6 ACCURACY DATA F THE AALYSIS F PQP AD AM Amount Std added µg/ml Total amount found µg/ml % Recovery µg/ml Mean PQP AM PQP AM PQP AM PQP AM
7 CCLUSI The Proposed RP-HPLC method is suitable for simultaneous determination of PQP and AM in Combined dosage form without any interferences form each other. The accuracy of the methods was assessed by recovery studies at three different levels. The method was found to be precise as indicated by the repeatability analysis, showing % RSD less than 2. All the parameters for both the drugs met the criteria of ICH guidelines for method validation. The developed method may be recommended for routine and QC analysis of the investigated drugs to provide simple, accurate and reproducible quantitative analysis for the determination of determination of PQP and AM in combined formulation. ACKWLEDGEMETS The authors are grateful to Management, Krupanidhi College of Pharmacy for their continuous support and encouragement and for providing the necessary facilities. REFERECES: 1. PubChem Compound [Cited 2013 july 31]; Available from: 2. PubChem Compound [Cited 2013 july 31]; Available from: 3. Tripathi KD Essentials of Medical Pharmacology. 6 th ed. Jaypee Brothers Medical Publishers ;2008.p Debrus B, Lebrun P, Kindenge JM, Lecomte F, Ceccato A, Caliaro G, MbayJMT, Boulanger B, Marini RD, Rozet E, Hubert Ph, (2011), Innovative high-performance liquid chromatography method development for the screening of 19 antimalarial drugs based on a generic approach, using design of experiments, independent component analysis and design space, J. of Chromatogr A, 1218, 31, Tarning J, Singtoroj T, Annerberg Ashton AM, Bergqvist Y, White J, Day PJ,. Lindegardh,(2006), Development and validation of an automated solid phase extraction and liquid chromatographic method for the determination of in urine, J of Pharm Biomed Ana, 41, 1(11), Te-Yu Hung, Davis TEM, Ilett KF,(2003), Measurement of piperaquine in plasma by liquid chromatography with ultraviolet absorbance detection, J. of Chromatogr B, 791, 1(2), Wahajuddin, Raju KSR, Taneja I, (2013), Bioanalysis of antimalarials using liquid chromatography, TrAC Trends in Ana Chem, 42,
8 8. Lindegardh, Annerberg A, White J, Day JP, (2008),Development and validation of a liquid chromatographic-tandem mass spectrometric method for determination of Piperaquine in plasma: Stable isotope labeled internal standard does not always compensate for matrix effects, J. of Chromatogr B, 862, 1(2), Hodel EM, Zanolari B, Mercier T, Biollaz J, Keiser J, lliaro P, Genton B, Decosterd LA, (2009), A single LC tandem mass spectrometry method for the simultaneous determination of 14 antimalarial drugs and their metabolites in human plasma, J. of Chromatogr B, 877, 10(1), Lindegardh, Tarning J, Toi PV, Hien TT, Farrar J, Singhasivanon P, White J, Ashton M, Day PJ, (2009), Quantification of artemisinin in human plasma using liquid chromatography coupled to tandem mass spectrometry, J. of Pharm Biomed Ana, 49(3), Kirchhofer C, Keiser J, Huwyler J, (2010), Development and validation of a liquid chromatography/mass spectrometry method for pharmacokinetic studies of Z78, a fasciocidal drug candidate, J. Chromatogr B, 878, (28), Amin CC, Blanchin MD, Ake M, Fabre H, (2013), Capillary electrophoresis methods for the analysis of antimalarials. Part II. Achiral separative methods, J. Chromatogr A, 1276, International Conference on Harmonisation (ICH) of technical requirements for registration of pharmaceuticals for human use: Validation of analytical procedures: Text and methodology Q2(R1),
INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES
International Journal of Universal Pharmacy and Bio Sciences 2(4): July-August 2013 INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES IMPACT FACTOR 1.89*** ICV 3.00*** Pharmaceutical Sciences
More informationJ Pharm Sci Bioscientific Res (4): ISSN NO
Development and Validation of Stability Indicating Analytical Method for Simultaneous Estimation of Perindopril and Potassium in Their Combined Marketed Dosage Form ABSTRACT: Gurjeet Kaur*, Nikhil Patel
More informationImpact factor: 3.958/ICV: 4.10 ISSN:
Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 99 Pharma Science Monitor 9(4), Oct-Dec 2018 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com
More informationValidated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form
International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.3, pp 1228-1232, July-Sept 2012 Validated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form Manzoor
More informationStability-indicating HPLC determination of tolterodine tartrate in pharmaceutical dosage form
Indian Journal of Chemical Technology Vol. 13, May 2006, pp. 242-246 Stability-indicating HPLC determination of tolterodine tartrate in pharmaceutical dosage form Vinay Saxena a *, Zahid Zaheer b & Mazhar
More informationResearch Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC
ISSN 2395-3411 Available online at www.ijpacr.com 248 Research Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC Mantha Vebkatesh* and A. Yasodha
More informationInternational Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010
International Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010 RP HPLC ESTIMATION OF EZETIMIBE IN TABLET DOSAGE FORMS NAGARAJU. P *, KRISHNACHAITHANYA. K, CHANDRABABU. D, SRINIVAS.
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD TO DETERMINE CINITAPRIDE HYDROGEN TARTARATE IN BULK AND PHARMACEUTICAL FORMULATION
Research Article ISSN:2230-7346 Journal of Global Trends in Pharmaceutical Sciences Vol.3, Issue 2, pp -619-627, April June 2012 DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD TO DETERMINE CINITAPRIDE HYDROGEN
More informationAnalytical Method Development and Validation of Lafutidine in Tablet dosage form by RP-HPLC
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3, No.3, pp 1403-1407, July-Sept 2011 Analytical Method Development and Validation of Lafutidine in Tablet dosage form
More informationJournal of Pharmaceutical and Biomedical Analysis Letters. Analysis Letters
Naga Jyothi. C et al, JPBMAL, 2015, 3(1): 242 246 ISSN: 2347-4742 Journal of Pharmaceutical and Biomedical Analysis Letters Journal Home Page: www.pharmaresearchlibrary.com/jpbmal Research Article Open
More informationRP-HPLC Method Development and Validation of Dapagliflozin in Bulk and Tablet formulation
221 IJPAR Volume 2 Issue 4 Oct - Dec -2013 ISSN: 2320-2831 Available Online at: [Research article] RP-HPLC Method Development and Validation of Dapagliflozin in Bulk and Tablet formulation Jeyabaskaran.M
More informationVolume 6, Issue 2, January February 2011; Article-015
Research Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE DETERMINATION OF DAPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION USING AN EXPERIMENTAL DESIGN Pratik Mehta*, Ujjwal Sahoo,
More informationStability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage form
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Stability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage form
More information7. Stability indicating analytical method development and validation of Ramipril and Amlodipine in capsule dosage form by HPLC.
7. Stability indicating analytical method development and validation of and in capsule dosage form by HPLC. 7.1 INSTRUMENTS AND MATERIALS USED 7.1.1 INSTRUMENTS 1. Shimadzu LC-2010 CHT with liquid chromatograph
More informationPharmacophore 2011, Vol. 2 (4), ISSN Pharmacophore. (An International Research Journal)
Pharmacophore 2011, Vol. 2 (4), 232-238 ISSN 2229 5402 Pharmacophore (An International Research Journal) Available online at http://www.pharmacophorejournal.com/ Original Research Paper SIMULTANEOUS ANALYSIS
More informationMethod Development and Validation Of Prasugrel Tablets By RP- HPLC
Method Development and Validation Of Prasugrel Tablets By RP- HPLC K.Sonia*, Ndwabe Hamunyare, K.Manikandan Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur,
More informationDevelopment and Validation of Stability-Indicating RP-HPLC Method for Estimation of Atovaquone
Available online at www.ijpcr.com International Journal of Pharmaceutical and Clinical Research 2012; 4(4): 68-72 Research Article ISSN 0975 1556 Development and Validation of Stability-Indicating RP-HPLC
More informationDepartment of Quality Assurance, Luqman College of Pharmacy, GULBARGA (K.S.) INDIA ABSTRACT
Int. J. Chem. Sci.: 12(3), 2014, 871-879 ISSN 0972-768X www.sadgurupublications.com DEVELPMENT AND VALIDATIN F A RAPID RP HPLC METHD FR THE DETERMINATIN F CINITAPRIDE HYDRGEN TARTARATE IN PURE AND ITS
More informationINTERNATIONAL RESEARCH JOURNAL OF PHARMACY
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 8407 Research Article STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF CEFIXIME
More informationANALYTICAL METHOD PROCEDURES
HPLC ASSAY AND RELATED SUBSTANCE Column Eurospher 100, C18, 25 x 0.40 cm 5µ Mobile Phase Buffer ph 2.0*: Acetonitrile (88:12 v/v) * Buffer ph 2 Potassium dihydrogen phosphate (KH 2 PO 4 ) - 0.68g Hepatane
More informationAvailable online at Scholars Research Library
Available online at www.derpharmachemica.com Scholars Research Library Der Pharma Chemica, 2012, 4 (3):1003-1015 (http://derpharmachemica.com/archive.html) ISSN 0975-413X CODEN (USA): PCHHAX Development
More informationA RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF PARA- PHENYLENEDIAMINE IN PURE FORM AND IN MARKETED PRODUCTS
A RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF PARA- PHENYLENEDIAMINE IN PURE FORM AND IN MARKETED PRODUCTS CH.MOUNIKA*, M.KINNERA Research Article SIR.C.R.REDDY COLLEGE OF PHARMACEUTICAL SCIENCES, ELURU.
More informationResearch Article. Figure 1. Chemical structure of doxofylline. Indonesian J. Pharm. Vol. 24 No. 1 : ISSN-p :
Research Article Indonesian J. Pharm. Vol. 24 No. 1 : 14 21 ISSN-p : 0126-1037 DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL
More informationReverse Phase High Performance Liquid Chromatography method for determination of Lercanidipine hydrochloride in bulk and tablet dosage form
Research Article ISSN: 0974-6943 M.V.Kumudhavalli et al. / Journal of Pharmacy Research 2014,8(11), Available online through http://jprsolutions.info Reverse Phase High Performance Liquid Chromatography
More informationChapter 4: Verification of compendial methods
Chapter 4: Verification of compendial methods Introduction In order to ensure accurate and reliable test results, the quality control laboratory (QCL) needs to use analytical methods (and accompanying
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2010, 2(5):399-417 Method develpopment and validation of Hydrochloride
More information*Author for Correspondence
DEVELOPMENT AND VALIDATION OF A SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF URAPIDIL HYDROCHLORIDE BOTH IN BULK AND PHARMACEUTICAL FORMULATION *S. Navgire 1, A. Ghadge 2, S. Gurav 2, S.
More informationSimultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(4), 1620-1625 Simultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium DESHMUKH HAFSA, S. CHANDA
More informationJournal of Drug Delivery and Therapeutics
Available online on 15.01.2018 at http://jddtonline.info Journal of Drug Delivery and Therapeutics Open Access to Pharmaceutical and Medical Research 2011-17, publisher and licensee JDDT, This is an Open
More informationInternational Journal of Pharma Research and Health Sciences. Available online at
CODEN (USA)-IJPRUR, e-issn: 2348-6465 International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Original Article Method Development and Validation for
More informationDETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
CHAPTER 9 DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 9 Determination of drug release during
More informationDissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form
Research Journal of Recent Sciences ISSN 2277-252 Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form Abstract Rele Rajan
More informationDevelopment and Validation of Stability Indicating RP-HPLC Method for the Determination of Anagrelide HCl in Pharmaceutical Formulation
ISSN 0976 3333 Available Online at www.ijpba.info International Journal of Pharmaceutical & Biological Archives 2013; 4(2): 342-346 ORIGINAL RESEARCH ARTICLE Development and Validation of Stability Indicating
More informationNovus International Journal of Analytical Innovations 2012, Vol. 1, No. 3
Novus International Journal of Analytical Innovations 2012, Vol. 1, No. 3 ISSN 2278-6953 www.novusscientia.org Accepted on October 22, 2012 RP-HPLC method for simultaneous estimation of Avitriptan and
More informationINTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND LIFE SCIENCES
A. Chenthilnathan et al IJRPLS, 2014, 2(2): 185-190 Research Article Available online at www.pharmaresearchlibrary.com/ijrpls ISSN: 2321-5038 INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND LIFE SCIENCES
More informationDevelopment and validation a RP-HPLC method: Application for the quantitative determination of quetiapine fumarate from marketed bulk tablets
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(1):142-146 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and validation a RP-HPLC method: Application
More informationScholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2011, 3 (6):49-57 (http://scholarsresearchlibrary.com/archive.html) ISSN 0974-248X USA CODEN: DPLEB4
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com HPLC AND UV-SPECTROPHOTOMETRIC ESTIMATION OF TENELIGLIPTIN
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
75 CHAPTER 3 DEVELOPMENT AND APPLICATION OF STABILITY-INDICATING HPLC METHOD FOR THE DETERMINATION OF NEVIRAPINE AND ITS IMPURITIES IN COMBINATION DRUG PRODUCT 3.1 INTRODUCTION OF DOSAGE FORM AND LITERATURE
More informationSaudi Journal of Medical and Pharmaceutical Sciences
Saudi Journal of Medical and Pharmaceutical Sciences Scholars Middle East Publishers Dubai, United Arab Emirates Website: http://scholarsmepub.com/ ISSN 2413-4929 (Print) ISSN 2413-4910 (Online) Stability
More informationDEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR IN-VIVO STUDY OF DICLOFENAC POTASSIUM
IJPSR (2013), Vol. 4, Issue 2 (Research Article) Received on 28 September, 2012; received in revised form, 24 November, 2012; accepted, 23 January, 2013 DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR
More informationInternational Journal of Current Trends in Pharmaceutical Research. International Journal of Current Trends in Pharmaceutical Research
International Journal of Current Trends in Pharmaceutical Research Journal Home Page: www.pharmaresearchlibrary.com/ijctpr Research Article Open Access Development and Validation Levofloxacin Andambroxol
More informationDEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR THE DETERMINATION OF AFLOQUALONE IN HUMAN PLASMA AND FORMULATION
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR THE DETERMINATION OF AFLOQUALONE IN HUMAN
More informationDevelopment and validation of RP-LC method for lisinopril dihydrate in bulk and its pharmaceutical formulations
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (7):340-344 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CDEN: DPLEB4
More informationA Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation
Pharmaceutical Research A Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation Manisha Puranik* a, Sailesh Wadher b and Kritika
More informationInternational Journal of Pharmaceutical Research & Analysis
149 International Journal of Pharmaceutical Research & Analysis e-issn: 2249 7781 Print ISSN: 2249 779X www.ijpra.com RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMBROXOL HYDROCHLORIDE
More informationR. Revathi et al. IRJP 1 (1)
ITERATIAL RESEARCH JURAL F PHARMACY Available online http://www.irjponline.com Research Article SPECTRPHTMETRIC ESTIMATI F GLICLAZIDE I BULK AD PHARMACEUTICAL DSAGE FRMS R Revathi*, V S Saravanan, P Mohan
More informationScholars Research Library. Rapid and sensitive RP-HPLC analytical method development and validation of Pioglitazone hydrochloride
Available online at www.scholarsresearchlibrary.com Der Pharmacia Lettre, 2011, 3(3): 128-132 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4 Rapid and sensitive RP-HPLC
More informationLiquid Chromatography and Spectroscopic Method for Estimation of Sulphadoxine in Marketed Formulation
Human Journals Research Article August 2018 Vol.:13, Issue:1 All rights are reserved by A. Yasodha et al. Liquid Chromatography and Spectroscopic Method for Estimation of Sulphadoxine in Marketed Formulation
More informationMETHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS
CHAPTER 6 METHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS School of Pharmaceutical Sciences, Vels University 106 METHOD DEVELOPMENT AND VALIDATION
More informationHPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ARTEROLANE MALEATE AND PIPERAQUINE PHOSPHATE IN BULK AND TABLET DOSAGE FORM
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ARTEROLANE MALEATE AND
More informationDevelopment and validation of stability indicating reverse phase high performance liquid chromatography method for Timolol Maleate
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.6, No.5, pp 1429-1435, Sept-Oct 2014 Development and validation of stability indicating reverse phase high performance
More informationDevelopment and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(2), 794-797. Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form D.SATHIS KUMAR
More informationInternational Journal of Pharma and Bio Sciences V1(1)2010. HPLC method for analysis of Lercanidipine Hydrochloride in Tablets
G. MUBEEN,, MAMTA PAL, AND M.N. VIMALA* Department of, Al-Ameen College of Pharmacy, Bangalore, India. * Corresponding author vimalamn_325@yahoo.co.in ABSTRACT A reverse phase HPLC method was developed
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF GABAPENTIN IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 10(4), 2012, 2209-2217 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF GABAPENTIN IN PURE AND PHARMACEUTICAL FORMULATIONS
More informationMethod development and validation for the estimation of metronidazole in tablet dosage form by UV spectroscopy and derivative spectroscopy
IJPAR Volume 3 Issue 2 April-June-2014 ISSN: 2320-2831 Available Online at: www.ijpar.com [Research article] Method development and validation for the estimation of metronidazole in tablet dosage form
More informationRP-HPLC Method Development and Validation for Simultaneous Estimation of Nadifloxacin and Adapalene in Bulk and Dosage Form
RP-HPLC Method Development and Validation for Simultaneous Estimation of and in Bulk and Dosage Form ABSTRACT: Khyati K. Patel 1, Atul Bendale 2, Shailesh V. Luhar 3, Sachin B. Narkhede 4 1. M.PHARM Student,
More informationChapter-4 EXPERIMENTAL WORK BY RP-HPLC
Chapter-4 EXPERIMENTAL WORK BY RP-HPLC 4.0 EXPERIMENTAL WORK BY RP-HPLC 4.1. DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR THE DETERMINATION OF NIFLUMIC ACID 4.1.1. MATERIALS AND METHODS OF NIFLUMIC
More informationAsian Journal of Research in Chemistry and Pharmaceutical Sciences Journal home page:
Research Article ISSN: 2349 7106 Asian Journal of Research in Chemistry and Pharmaceutical Sciences Journal home page: www.ajrcps.com ESTIMATION OF RAMELTEON IN TABLET DOSAGE FORM BY HPLC M. Jyothsna*
More informationTEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD
APPENDIX V TEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD Validating Chromatographic Methods. By David M. Bliesner Copyright 2006 John Wiley & Sons, Inc. 159 160 APPENDIX V Title: Effective: Document No:
More informationSimultaneous estimation and validation of bromhexine and cephalexin in bulk and pharmaceutical dosage form by RP-HPLC method
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2014, 6 (6):315-321 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationRP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.ejchem.net 2012, 9(3), 1407-1411 RP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms M. VIJAYA LAKSHMI 1, J.V.L.N.S. RAO 2 AND A. LAKSHMANA
More informationResearch Article Available online at
Research Article Available online at www.jgtps.com ISSN: 2230-7346 Volume- 5, Issue -2, pp-1522-1527- April June (2014) DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CITICOLINE
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(10):483-494 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Validated RP-HPLC method for the simultaneous determination
More informationValidated First Order Derivative Spectroscopic Method for the determination of Stavudine in Bulk and Pharmaceutical Dosage Forms
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.3, No.1, pp 18-22, Jan-Mar 2011 Validated First Order Derivative Spectroscopic Method for the determination of Stavudine
More informationDevelopment and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method
21 Article Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method Mali Audumbar Digambar*, Jadhav Santosh, Mane Pandurang,
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(1):34-39 ISSN: 0976-8688 CODEN (USA): PSHIBD Advance simultaneous determination of paracetamol, thiocolchicoside and aceclofenac
More information462 1 & (2&3 ( 4 5 6" 6 7 ' ("0 / L1 1 % FG &
. 2009 *!" % & ' #$. %* () 1 () -6-"/ (0 42. / & ' :', 462 1 & (2&3 ( 4 5 6" 6 7 ' ("0 / () 0?
More informationSravani and Haritha Indian Journal of Research in Pharmacy and Biotechnology ISSN: (Print) ISSN: (Online)
A new development and validated RP-HPLC method for the assay and related substances of Itraconazole in capsule dosage form Sarvani Paruchuri*, Haritha Pavani K Nimra College of Pharmacy, Vijayawada, Andhra
More informationDepartment of Chemistry, JNTUACE, Kalikiri
Method Development and Validation of Aegle marmeleous M. Swetha 1*, N. Saritha 1, N. Devanna 2 1 Department of Chemistry, JNTUACE, Kalikiri.-517234 2 Department of Chemistry, JNTUA, Anthapuramu -515002
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(1): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(1):118-122 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of High Performance Liquid
More informationHPLC Method Development and Validation for the estimation of Esomeprazole in Bulk and Pharmaceutical Dosage Form
International Journal of Drug Development & Research October-December 2012 Vol. 4 Issue 4 ISSN 0975-9344 Available online http://www.ijddr.in Covered in Official Product of Elsevier, The Netherlands SJR
More informationImpact factor: 3.958/ICV: 4.10 ISSN:
Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 164 Pharma Science Monitor 9(2), Apr-Jun 2018 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com
More informationInternational Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:
Research Article CODEX: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE
More informationCHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC. Diclofenac (gift sample from M/s Micro Labs Ltd., Pondicherry)
CHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC A UV spectrophotometric method based on the measurement of absorbance at 276nm in phosphate buffer of p H 7.4 was used in the present study of the
More informationDevelopment of Validated Analytical Method of Mefenamic Acid in an Emulgel (Topical Formulation)
Development of Validated Analytical Method of Mefenamic Acid in an Emulgel (Topical Formulation) TEENA OSWAL*, DR.SURYAKANT BHOSALE, DR. SONALI NAIK MET Institute Of Pharmacy Met Complex, Bandra Reclamation,
More informationIntercontinental journal of pharmaceutical Investigations and Research
Kranthi K K et al, ICJPIR 2017, 4(1), XXX-XXX Available online at ISSN: 2349-5448 Intercontinental journal of pharmaceutical Investigations and Research ICJPIR Volume 4 Issue 1 Jan Mar- 2017 Research Article
More informationInternational Journal of Research and Reviews in Pharmacy and Applied science.
International Journal of Research and Reviews in Pharmacy and Applied science www.ijrrpas.com Corresponding Author NARENDRA DEVANABOYINA* Ch.DURGAKUMARI, L.NAGARAJU, R.SRIDHARANI PRIYA, S.BHAVITHA Department
More informationReceived: ; Accepted:
International Journal of Universal Pharmacy and Bio Sciences 1(2): November-December2012 INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES Pharmaceutical Sciences Research Article!!! Received:
More informationDEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR DETERMINATION OF LOPINAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR DETERMINATION OF LOPINAVIR IN BULK AND
More informationValidation of Stability-Indicating RP-HPLC Method for the Assay of Ibrutinib in Pharmaceutical Dosage form
Validation of Stability-Indicating RP-HPLC Method for the Assay of Ibrutinib in Pharmaceutical Dosage form 8.1 Introduction Ibrutinib (IBR) is an anticancer drug targeting B-cell malignancies (blood cancer
More informationAnalytical method development and method validation for the estimation of pantoprazole in tablet dosage form by RP-HPLC
Available online at www.derpharmachemica.com Scholars Research Library Der Pharma Chemica, 2013, 5(4):99-104 (http://derpharmachemica.com/archive.html) ISSN 0975-413X CODEN (USA): PCHHAX Analytical method
More informationRevision Bulletin 27 Jan Feb 2017 Non-Botanical Dietary Supplements Compliance
Niacin Extended-Release Tablets Type of Posting Posting Date Official Date Expert Committee Reason for Revision Revision Bulletin 27 Jan 2017 01 Feb 2017 Non-Botanical Dietary Supplements Compliance In
More informationDevelopment And Validation Of Rp-Hplc Method For Determination Of Velpatasvir In Bulk
International Journal of Engineering Science Invention (IJESI) ISSN (Online): 2319 6734, ISSN (Print): 2319 6726 www.ijesi.org PP. 36-41 Development And Validation Of Rp-Hplc Method For Determination Of
More informationSTABILITY INDICATING METHOD OF RELATED IMPURITIES IN VENLAFAXINE HYDROCHLORIDE SUSTAINED RELEASE TABLETS
Issn No: 976-39 RESEARCH ARTICLE STABILITY INDICATING METHOD OF RELATED IMPURITIES IN VENLAFAXINE HYDROCHLORIDE SUSTAINED RELEASE TABLETS CHETLAPALLI SATYA SRINIVAS 1, P.RENUKA DEVI 2 and GAMPA VIJAYAKUMAR*
More informationInternational Journal of Medicine and Pharmaceutical Research
International Journal of Medicine and Pharmaceutical Research Journal Home Page: www.pharmaresearchlibrary.com/ijmpr Research Article Open Access Development of Validated Stability Indicating Assay Method
More informationB. Anupama et al. IRJP 2 (1)
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY ISSN 223 847 Available online http://www.irjponline.com Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ANALYSIS OF RACECADOTRIL IN PURE AND
More informationAppendix II- Bioanalytical Method Development and Validation
A2. Bioanalytical method development 1. Optimization of chromatographic conditions Method development and optimization of chromatographic parameters is of utmost important for validating a method in biological
More informationRevision Bulletin 29 Dec Jan 2018 Non-Botanical Dietary Supplements Compliance
Niacin Extended-Release Tablets Type of Posting Posting Date Official Date Expert Committee Reason for Revision Revision Bulletin 29 Dec 2017 01 Jan 2018 Non-Botanical Dietary Supplements Compliance In
More informationJournal of Advanced Scientific Research DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-HPLC METHOD FOR ESTIMATION OF DABIGATRAN ETEXILATE
Damle et al, J Adv Sci Res, 2014, 5(3): 39-44 39 Journal of Advanced Scientific Research Available online through http://www.sciensage.info/jasr ISSN 0976-9595 Research Article DEVELOPMENT AND VALIDATION
More informationAnalytical method development and validation of gabapentin in bulk and tablet dosage form by using UV spectroscopic method
IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: www.ijpar.com ISSN:2320-2831 Research article Open Access Analytical method development and validation of gabapentin in bulk and tablet dosage
More informationUSP Method Transfer and Routine Use Analysis of Irbesartan Tablets from HPLC to UPLC
USP Method Transfer and Routine Use Analysis of Tablets from HPLC to UPLC Aparna Chavali, Tanya Jenkins and Patricia McConville Waters Corporation, 34 Maple Street, Milford, MA USA APPLICATION BENEFITS
More informationInt. J. Pharm. Sci. Rev. Res., 30(2), January February 2015; Article No. 09, Pages: 63-68
Research Article Stability indicating RP-HPLC Method for Determination of FexoFenadine Hydrochloride and its Related Substances in Active Pharmaceutical Substance Abhay Gupta* 1, Dr. Birendra Srivastava,
More informationA NEW HPLC METHOD FOR THE QUANTIFICATION OF PANTOPRAZOLE IN PHARMACEUTICALS
Int. J. Chem. Sci.: 6(2), 2008, 579-586 A NEW HPLC METHOD FOR THE QUANTIFICATION OF PANTOPRAZOLE IN PHARMACEUTICALS K. BASAVAIAH, U. R. ANIL KUMAR and K. THARAPA Department of Chemistry, University of
More informationInternational Research Journal of Pharmaceutical and Applied Sciences Available online at Int. Res J Pharm. App Sci.
International Research Journal of Pharmaceutical and Applied Sciences Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(4):49-55 Research Article Method development and validation
More informationReceived: ; Accepted:
International Journal of Institutional Pharmacy and Life Sciences 2(1): January-February 2012 INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES Pharmaceutical Sciences Research Article!!!
More informationKEYWORDS: Acetaminophen, Doxylamine succinate, Dextromethorphan hydrobromide.
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 6718, ISSN (Print): 2319 670X Volume 3 Issue 7 July 2014 PP.08-12 Analytical method development and validation of acetaminophen,
More informationJournal of Chemical and Pharmaceutical Research, 2012, 4(6): Research Article. Estimation of zaleplon by a new RP-HPLC method
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(6):3010-3014 Research Article ISS : 0975-7384 CODE(USA) : JCPRC5 Estimation of zaleplon by a new RP-HPLC method Tentu.
More informationSTABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG AND IT S PHARMACEUTICAL DOSAGE FORM
International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 3, Issue 3, 2011 Research Article STABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG
More informationANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF IVABRADINE HCL IN BULK AND FORMULATION
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF IVABRADINE HCL IN BULK AND FORMULATION Gauri Korgaonkar *, Vaishali B. Jadhav, Ashish Jain Shri d.d. vispute college of pharmacy & research center, Panvel,
More information