Ultrasonic Studies On Interionic Interactions Of Potassium Chloride In Aqueous Lactose Solution At Varying Molalities And Temperatures
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1 International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : Vol.5, No.4, pp , April-June 2013 Ultrasonic Studies On Interionic Interactions Of Potassium Chloride In Aqueous Lactose Solution At Varying Molalities And Temperatures Shashi Kant*, Parul, Kamini Sharma Department of Chemistry, Himachal Pradesh University, Summer Hill, Shimla , INDIA. Corres.author: sunshine.kamini@gmail.com Abstract: The present experimental investigation was carried out in order to explore the possible molecular interionic interactions of potassium chloride in aqueous lactose solution at , K, and K. Experimental values of density (d), viscosity (η) and ultrasonic velocities (U) were carried out on the liquid ternary mixtures of water +lactose + potassium chloride. The binary solvent mixture of water + lactose was prepared under molality(m) basis. Potassium chloride was added under different molalities with these binary solvent mixtures. The related and relevant parameters correlated to our present study such as adiabatic compressibility, change and relative change in adiabatic compressibility, acoustic impedance (Z), intermolecular free length (L f ), Wada s constant (W), relative association (R.A.), relaxation time (τ) were determined. The present investigation has exploited the possible molecular associations such as ion-ion, ionsolvent, solute-solvent, solute-solute etc., which are identified and eventually discussed about the behavior of solute (potassium chloride) in the solvent mixture. Keywords: Ultrasonic velocity, lactose, potassium chloride, adiabatic compressibility, free length, relaxation time. Introduction: Saccharides are very important for some physiological processes. They are not only the basic material for energy metabolism in organisms, but also play a significant role in the configuration of biological molecules [1, 2]. The study ofcarbohydrates / saccharides has become a subject of increasing interest because of the multidimensional physical, biochemical and industrially useful properties of these compounds [3-9]. In addition to their importance in the food, pharmaceutical and chemical industries, saccharides have received considerable attention for their ability to protect biological macromolecules [10, 11]. Sugars and polyols are well known stabilizing agents of proteins/enzymes [12, 13] in their native state owing to their ability to enhance the structure of water. Saccharides and their derivatives as the most abundant class of biomolecules are known to exist in wide range of forms, which is a reflection of their biological versatility and the great diversity of their biological functions such as structural, protective metabolic and recognition. The saccharide components of cell membranes are the receptors of biologically active components (enzymes, drugs etc.). Saccharides are able to stabilize the native state of proteins/enzymes [14-16]. Interactions of electrolytes with saccharides are very important in exploring the stability of polysaccharides in biological systems as well as in the chemical industry of saccharides. It is an essential component for maintaining cell viability, a natural cell-protecting agent, as well as an energy reservoir in many organisms [17]. It has been found that thedecomposition, synthesis, metabolism and transmembrane transport of saccharides have relationships with the concentration of metal ions in body-
2 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) 1949 fluid. So the study of the interaction between alkali metal halides and aqueous carbohydrates is valuable to examine the influence of electrolytes on some physiological behavior and helpful to understand the essence of some biological phenomena. The present study investigates the behavior of potassium chloride in different composition of lactose by using acoustic measurements. In this paper, we report the densities and ultrasonic studies at different temperatures (i.e K) over a wide concentration range. These data were used to calculate various acoustic parameters like adiabatic compressibility, change and relative change in adiabatic compressibility, acoustic impedance (Z), intermolecular free length (L f ), Wada s constant (W), relative association (R.A.), relaxation time (τ). These parameters are discussed in terms of various solute cosolute interactions in aqueous solutions; thus the study contributes to a better understanding of the interactions taking place between solute and solvent. Experimental: Water used for solutions had specific conductance in the range x cm -1. Potassium chloride and lactose (Anala R) were dried over anhydrous calcium chloride for more than 48h and used as such. All the solutions were prepared by weight and conversion of molality to molarity was done by using the standard expression [18]. The concentration range of potassium chloride in 2, 4 and 6 wt. % of lactose was 0.01 to 0.12 m. The density and ultrasonic velocity was measured with the help of DSA (Density and Sound A nalyser) 5000, Anton Paar, GmbH, Garz, Austria. Theory And Calculations: Using the measured data, some acoustical parameters such as adiabatic compressibility (), change () and relative change in adiabatic compressibility ( / o ), acoustic impedance (Z), intermolecular free length (L f ), Wada s constant (W), relative association (R.A.), relaxation time (τ) were studied and evaluated by using the standard equations: 1 Adiabatic compressibility () = 2 U Where U is the ultrasonic velocity and is the density. Change in adiabatic compressibility () = (- o ) (2) Where and o are the adiabatic compressibility of solution and solvent respectively. Relative change in adiabatic compressibility = (/ o ) (3) Acoustic impedance (Z) = U (4) Intermolecular free length (L f ) = K 1/2 (5) Wada s constant (W) = M 1 / 7 (1) (6) Relative association (R.A.) = 4 Relaxation time (τ) = 2 3U o U U o 1/ 3 (7) (8)
3 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) 1950 Results And Discussion: The experimental values of density (), ultrasonic velocity (U), adiabatic compressibility, change and relative change in adiabatic compressibility for different molal compositions of potassium chloride in 2, 4 and 6 wt. % lactose at different temperatures (i.e k k) are shown in Table-1. The results show that densities and ultrasonic velocities increases with increase in the concentration of lactose as well as with the concentration of potassium chloride. Moreover, the density decreases with increasing temperature in all the systems while the opposite trends are observed for ultrasonic velocity. The increase of ultrasonic velocity with temperature in all systems indicates a weakening of solute solvent interactions at higher temperatures. As the temperature increases, hydrogen bonds between water and lactose molecules break. It is clear from the Table-1 that the values of adiabatic compressibility () seems to decrease with increase of the solute content as well as with the rise in temperature. Such a decrease in adiabatic compressibility observed in the present system, generally, confirms the conclusions drawn from the velocity data. The increasing electrostrictive compression of water around the molecules may result in a large decrease in the compressibility of the solutions. The decrease in is due to the increase in electrostriction compression of solvent around the molecules which results in a large decrease in the compressibility of solutions [19]. The compressibility appears to be decreasing with decrease in hydrogen bond strength formed by solute and solvent molecules. The behavior of compressibility depicts the existence of interaction between solute and solvent molecules in which the structural arrangement in the neighbourhood of consistent solutes is considerably affected. The sample plots of β Vs m for KCl in 2% lactose at different temperatures is shown in fig (1). The negative values of change in adiabatic compressibility () and relative change in adiabatic compressibility (/ o ) are due to solute-solvent interactions [20-21].Such an increase in and / o values with increase in concentration may be attributed to an overall increase in the cohesive forces in the solution[22]. These cohesive forces may be due to the interactions in the solution. The acoustic impedance (Z) of all the systems is found to increase with increase in the concentration of potassium chloride in 2, 4 and 6 wt. % lactose. The sample plots of Z vs m for KCl in 2% lactose at different temperatures is shown in fig (2). This is in agreement with the theoretical requirements as U and both increases with increase in the concentration of the solute. This increase of Z values with solute concentration can be attributed to the effective solute-solvent interactions. A similar type of behavior has been obtained for tetra alkyl ammonium and alkali metal salts in methanol + chloro benzene mixtures [23]. Intermolecular free length (L f ) was obtained from adiabatic compressibility () using equation[24]: Intermolecular free length (L f ) = K 1/2 Where K is the temperature dependent constant (= ( T) 10-8 ))[25]. It is clear from table that ultrasonic velocity (U) increases and intermolecular free length (L f ) decreases with increase in concentration of potassium chloride in 2, 4 and 6 wt.% lactose at different temperatures (i.e k k). In general U and L f have been reported to vary as the inverse of each other as in the present systems [26-27].The decrease in the value of L f with the increase in molality indicates the presence of significant ion-solvent interaction between solute and solvent molecules due to which the structural arrangement in the neighbourhood of constituent ions is considerably affected [28]. The sample plots of L f vs m for KCl in 2% lactose at different temperatures is shown in fig (3). Wada s constant (W) has been calculated by standard equation [29]: Wada s constant (W) = M 1/ 7 It has been found that the values of Wada s constant (W) decreases with increase in concentration of solute and increases with increase in temperature. To obtain a firm impact of interactions in solutions, relative association (R.A.) was calculated by following relation[30]: Relative association (R.A.) = o U U o 1/ 3 where o and Uo are the density and ultrasonic velocity of solvent respectively. Relative association is influenced by two factors (i) breaking up of the associated solvent molecules on addition of solute in it and (ii) the solvation of solute molecules. The former leads to decrease and later to increase of relative association. In
4 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) 1951 the present study, the values of (R.A.) increase with increase in solute concentration showing significant ionsolvent interactions which increase with increase in solute concentration [23]. The sample plots of τ Vs m for KCl in 2% lactose at different temperatures is shown in fig (4). Table-1: Density (), ultrasonic velocity (U), adiabatic compressibility (), change () and relative change in adiabatic compressibility (/ o ) for potassium chloride in 2, 4 and 6 wt. % Lactose at different temperatures (i.e K, K, K, K) Molality (m) x 10-3 (Kg m -3 ) U (ms -1 ) x10 10 (Pa -1 ) -x (Pa -1 ) -/ o x10 3 Potassium chloride in 2% aqueous Lactose Potassium chloride in 4% aqueous Lactose
5 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) Potassium chloride in 6% aqueous Lactose
6 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) Table-2: Acoustic impedance (Z), intermolecular free length (L f ), Wada s constant (W), relative association (R.A.), relaxation time (τ) for Sodium chloride in 2, 4 and 6 wt. % Lactose at different temperatures (i.e K, K, K, K) Molality (m) Z x 10-6 (Kg m -2 s -1 ) L f x (m) W x 10 4 (m 3 mol -1 Pa 1/7 ) R.A τ X (s) Potassium chloride in 2% aqueous Lactose
7 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) Potassium chloride in 4% aqueous Lactose Potassium chloride in 6% aqueous Lactose
8 X1007(Pa-1Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) K K K K )molality (mol kg-1) Fig 1: Plots of β vs m for KCl in 2% lactose at different temperatures.
9 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) 1956 Z X 10 6 (kg m -2 s -1 ) K K K K molality (mol kg -1 ) Fig 2: Plots of Z vs m for KCl in 2% lactose at different temperatures. L f X (m) K K K K molality (mol kg -1 ) Fig 3: Plots of L f vs m for KCl in 2% lactose at different temperatures.
10 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) 1957 x (s) K K K K molality (mol kg -1 ) Fig 4: Plots of τ vs m for KCl in 2% lactose at different temperatures. Conclusion: In the light of the above discussion, it may be concluded that there are existence of powerful molecular interactions in the systems studied. Both the solute-solute interactions and solute-solvent interactions are possible in the systems. There is uniform increase in density and decrease in intermolecular free length with increase in concentration indicating the loosening of intermolecular forces due to thermal agitation of the molecules in aqueous lactose at different temperatures (i.e k k). References: [1] Barone G.,Physical chemistry of aqueous solutions of oligosaccharides, Thermochim. Acta., 1990, 162, [2] ZhuoK., Wang J., Cao Y. and Lu J., Thermodynamics of the Interaction of HCl with D-Fructose in Water, J. Phys. Chem. B., 1998, 102, [3] Goldberg R. N. and Tewari Y. B., Thermodynamic and transport properties of carbohydrates and their monophosphates: the pentoses and hexoses, J. Phys. Chem. Ref. Data., 1989, 18, [4] Boerio-Goates J.,Heat-capacity measurements and thermodynamic functions of crystalline α-[d]-glucose, J. Chem. Thermodyn., 1991, 23, [5] PutnamR. L. and Boerio-Goates J.,Heat capacity measurements and thermodynamic functions of crystalline [D]-sucrose, J. Chem. Thermodyn., 1993, 25, [6] Goldberg R. N. and Tewari Y. B.,A calorimetric and equilibrium investigatino of the hydrolysis of lactose,j. Biol. Chem., 1989, 264, [7] Goldberg R. N., Tewari Y. B., and Ahluwalia J. C., Thermodynamics of the hydrolysis of sucrose, J. Biol. Chem., 1989, 264, [8] Goldberg R. N. and Tewari Y. B., Thermodynamics of hydrolysis of disaccharides. Lactulose, alpha-dmelibiose, palatinose, D-trehalose, D-turanose and 3-o-beta-D-galactopyranosyl-D-arabinose, Biophys. Chem., 1991, 40, [9] Birch G. G. and Shamil S.,Structure sweetness and solution properties of small carbohydrate molecules,j. Chem. Soc., Faraday Trans., 1988, 84, [10] Arakawa T., Kita Y. and Carpenter J. F.,Protein-solvent interactions in pharmaceutical formulations, Pharm. Res., 1991, 8,
11 Shashi Kant et al /Int.J.ChemTech Res.2013,5(4) 1958 [11] Miller D. P. and De Pablo J. J.,Calorimetric solution properties of simple saccharides and their significance for the stabilization of biological structure and function, J. Phys. Chem. B., 2000, 104, [12] Gupta M. N.,Thermostabilisation of proteins, Biotechnol. Appl. Biochem., 1991, 14, [13] Timasheff S. N. and Arakawa T., Stabilization of protein structure by solvents, IRL Press, Oxford, 1989, [14] Zhuo K., Liu G., Wang Y., Qiuhe R. and Wang J.,Activity coefficients and conductivities of calcium nitrate in glucose/galactose water mixtures,fluid Phase Equilibria, 2007, 258, [15] Ernst B., Hart G. W. and Sinay P., Carbohydrates in Chemistry and Biology, Eds.; Wiley-VCH: Weinheim, New York, 2000, [16] MillerD. P., De Pablo J. J., and Corti H. R., Viscosity and Glass Transition Temperature of Aqueous Mixtures of Trehalose with Borax and Sodium Chloride, J. Phys. Chem. B., 1999, 103, [17] ZhuoR., Liu H., Zhang H. and Wang Y., Activity Coefficients and Volumetric Properties for the NaI + Maltose + Water System, J. Chem. Eng. Data, 2008, 53, [18] Ward G.K. and Millero F.J., The effect of pressure on the ionization of boric acid in aqueous solutions from molal-volume data, J. Soln. Chem., 1974, 3, 417. [19] Riyazudden and Khan Imran, Interactions in L-alanine /L-proline /L-valine /L-leucine aqueous KCl/KNO 3 systems at different temperatures: An isentropic compressibility study, J. ThermochimicaActa, 2009, 483, 45. [20] Aswar A. S., Interaction studies on biomolecules in aqueous medium, J. Pure Appl. Ultrasonics, 1998, 20, 82. [21] Ali A., Nain A.K., Kumar N. and Ibrahim M., Study of molecular interactions in binary mixtures of acetonitrile with amides through ultrasonic speed measurements, J. Pure Appl. Ultrasonics, 2002, 24, [22] PandeyD. and Akhtar Y., Ultrasonic studies of aqueous concentrated electrolytic solutions at K, J. Pure Appl. Ultrasonics, 1996, 18, [23] Syal V.K., Lal G., Bisht P., Chauhan S., Ultrasonic measurements of some 1:1 electrolytes in chlorobenzene + methanol mixtures, J. Mol. Liquids, 1995, 63, [24] Mehta N.M., Karia F.D., Parsania P.H., Effect of temperature on ultrasonic velocity and thermodynamic parameters of bisphenol-c-formaldehyde-acrylate resin solutionsfluid Phase Equilibria, 2007, 262, [25] Ali A., Nain A. K., Kamil M.,Physico-chemical studies of non-aqueous binary liquid mixtures at various temperatures, ThermochimicaActa, 1996, 274, [26] Endo Harumi, NomotoOtohiko, Sound Velocity in Aqueous Non-Electrolyte Solutions, Bulletin of the Chemical Society of Japan, 1976, 49(10), [27] Azhagiri S., Jayakumar S., Padmanaban R., Gunasekaran S. andsrinivasan S., Acoustic and Thermodynamic Properties of Binary Liquid Mixtures of Benzaldehyde in Hexane and Cyclohexane, J. Solution Chem., 2009, 38, [28] Reddy R.R., Rama Gopal K., Narasimhulu K., Siva Sankara Reddy L., Kumar K. Raghavendra, Venkatesulu A., Krishna Reddy C.V., Correlations between Moelwyn Hughes parameter, available volume and intermolecular free-lengths in liquid systems, J. Mol. Liq., 2008, 140, [29] Yadava S.S., YadavAniruddh, Ultrasonic study on binary liquid mixtures between some bromoalkanes and hydrocarbons, Ultrasonics, 2005, 43, [30] SinhaAnuradha, Roy M.N., Densities, Viscosities, and Sound Speeds of Some Acetate Salts in Binary Mixtures of Tetrahydrofuran and Methanol, J. Chem. Eng. Data, 2006, 51, *****
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