SCES2211/ SCES2241 : KIMIA ANALISIS I/ KIMIA ANALISIS ASAS ANALYTICAL CHEMISTRY I/ BASIC ANALYTICAL CHEMISTRY

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1 UNIVERSITI MALAYA UNIVERSITY OF MALAYA PEPERIKSAAN IJAZAH SARJANA MUDA SAINS EXAMINATION FOR THE DEGREE OF BACHELOR OF SCIENCE PEPERIKSAAN IJAZAH SARJANA MUDA SAINS DENGAN PENDIDIKAN EXAMINATION FOR THE DEGREE OF BACHELOR OF SCIENCE WITH EDUCATION SESI AKADEMIK 2014/2015 : SEMESTER 1 ACADEMIC SESSION 2014/2015 : SEMESTER 1 SCES2211/ SCES2241 : KIMIA ANALISIS I/ KIMIA ANALISIS ASAS ANALYTICAL CHEMISTRY I/ BASIC ANALYTICAL CHEMISTRY Disember 2014/Januari 2015 December 2014/January 2015 MASA : 2 jam TIME : 2 hours ARAHAN KEPADA CALON : INSTRUCTIONS TO CANDIDATES : Kertas soalan ini mengandungi Bahagian A dan B. This paper consists of Section A and B. Jawab soalan mengikut arahan yang diberikan dalam setiap bahagian. Answer the questions according to the instructions given in each section. (Kertas soalan ini mengandungi 9 soalan dalam 6 halaman yang dicetak) (This question paper consists of 9 questions on 6 printed pages)

2 BAHAGIAN A (50 MARKAH) SECTION A (50 MARKS) Jawab semua Lima (5) soalan daripada bahagian ini Answer all Five (5) questions from this section 1. (a) Bagaimanakah untuk meningkatkan kecekapan pengekstrakan dalam pemisahan fasa cecair-cecair? How to improve the efficiency of extraction in liquid-liquid phase separation? D = 3.5; V aq = V org = 10 ; p = D D+( v aq Vorg ) (2 markah/ marks) Jika kita perlu untuk mencapai pemulihan 99%, kira berapa banyak pengekstrakan diperlukan. If we need to achieve 99% recovery, calculate how many extractions are required. (c) Berdasarkan keputusan yang diperolehi dari, bagaimanakah anda boleh menyesuaikan kaedah untuk mencapai pemulihan 99% pada tepat 3 pengekstrakan? Based on the result obtained from, how could you adjust the method to achieve 99% recovery in exactly 3 extractions? 2/6

3 2. (a) Rajah 1 menunjukkan instrumen HPLC. Isikan tempat kosong. Figure 1 is HPLC instrument set-up. Fill in the blanks. SCES2211/SCES Rajah 1 Figure 1. (6 markah/ marks) What are the importance of HPLC technique? Apakah kepentingan teknik HPLC? 3. (a) Apakah ph yang ideal untuk pentitratan ion logam dengan EDTA dalam pentitratan kompleksometri? Mengapa? What is the ideal ph for the titration of a metal ion with EDTA in complexometric titration? Why? (2 markah/ marks) Mengapakah larutan ammonia tidak sesuai untuk digunakan untuk menyesuaikan ph dalam pentitratan kompleksometri? Why ammonia solution is not suitable to be used to adjust ph in complexometric titration? (3 markah/ marks) 3/6

4 (c) Bagaimanakah meningkatkan pemilihan pengesanan ion logam dalam pentitratan kompleksometri? How to improve the selectivity of metal ion detection in complexometric titration? 4. (a) Bagaimanakah sebuah ion elektrod selektif (ISE) berfungsi untuk mengesan ion tertentu dalam larutan sampel? How does an ion selective electrode (ISE) function in order to detect a specific ion in a sample solution? (2 markah/ marks) Namakan empat jenis membran ISE. Name four types of ISE membrane. (c) Namakan empat contoh komersial ISE. Name four examples of commercial ISE. 5. (a) Apakah ciri-ciri gelombang voltametri siklik (CV) berbalik? What are the characteristics of a reversible cyclic voltametry (CV) wave? Dalam sistem berbalik, apakah kesan dari kadar menyapu ( ) dan kinetik, terhadap CV? In a reversible system, what is the effect of sweep rate ( ) and kinetics, respectively to CV? 4/6

5 BAHAGIAN B (50 MARKAH) SECTION B (50 MARKS) Jawab SEMUA soalan. Answer ALL questions. 6. (a) Nyatakan Hukum Beer. Terangkan hubungan di antara transmitan dan keserapan. State Beer s Law. Explain the relationship between transmittance and absorbance (7 markah/ marks) Satu sebatian tak diketahui X mempunyai berat molekul dilarutkan dalam satu kelalang volumetrik berukuran 5-mL. Sebanyak 1.00 ml alikuot dimasukkan ke dalam kelalang volumetrik 10-mL, dicairkan hingga ke tanda senggatan. Keserapan yang diukur menggunakan sel 1-cm pada 340 nm ialah Keterserapan molar bagi X ialah 6,130 M -1 cm -1. Tentukan kepekatan sebatian X. An unknown compound of X with a molecular weight of was dissolved in a 5-mL volumetric flask ml of the aliquot was added into a 10-mL volumetric flask, was diluted to the mark. The absorbance measured using a 1-cm cell at 340 nm was The molar absorptivity of X was 6,130 M -1 cm 1. Determine the concentration of compound X. 7. (a) Apakah jenis molekul yang teruja dengan radiasi UV? Jelaskan. What types of molecules are excited by UV radiation? Explain. (8 markah/ marks) Apakah kengkangan spektroskopi UV sebagai alat bagi analisis quantitative? What are the limitations of UV spectroscopy as a tool for a quantitative analysis? 8. Lukis dan labelkan gambarajah blok bagi alat UV-Vis spektrofotometer. Terangkan kepentingan setiap komponen utama dalam peralatan ini. Draw and label a block diagram of UV-Vis spectrophotometer. Describe the purpose of each major component of this instrument. (10 markah/ marks) 5/6

6 9. (a) Nyatakan perbezaan antara istilah berikut: State the difference between the following terms: (i) (ii) (iii) Ketepatan dan kejituan Accuracy and precision Ralat tentu dan ralat tak tentu Determinate error and indeterminate error Ujian-t dan Ujian-Q t-test and Q-test (9 markah/ marks) Kandungan karbohidrat bagi glikoprotein diperolehi sebanyak 11.5, 12.7, 13.0, 11.9 dan 12.6 g karbohidrat bagi setiap 100 g protein dalam analisis replikat. Hitung sela keyakinan bagi kandungan karbohidrat pada aras keyakinan 95%. Carbohydrate content for glycoprotein obtained were 11.5, 12.7, 13.0, 11.9 and 12.6 g carbohydrate for each 100 g of protein in replicate measurements. Calculate confidence interval for carbohydrate content at 95% confidence level. TAMAT END (6 markah/ marks) 6/6

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