PLGA Nanoparticles Formulation

Size: px
Start display at page:

Download "PLGA Nanoparticles Formulation"

Transcription

1 PLGA Nanoparticles Formulation Using NanoAssemblr TM Benchtop Introduction Advancements in chemistry have led to the discovery of many promising new drug candidates, however, nearly 9% of these molecules are poorly water soluble, leading to their inability to transition from discovery to clinic. With this in mind, the focus of research has shifted towards the development of various drug delivery technologies to enable new drug candidates to reach the market. One of these drug delivery technologies is nanomedicine [1-3]. Nanotechnology has enabled the manipulation of materials at the atomic scale. Nanoparticles can improve the solubility of poorly soluble drugs and can interact with biological systems at the sub-cellular level maximizing their therapeutic benefits over conventional forms of delivery [1, 4]. Research on synthetic biodegradable polymers has gained momentum in drug delivery applications due to their biocompatibility and biodegradability. Amongst these polymers, poly(lactic-co-glycolic acid) (PLGA) is one of the most attractive polymers for drug delivery as it has been approved by the US FDA and the European Medical Agency (EMA) in parenteral drug delivery systems [5]. However, even after approval there is a lack of PLGA nanoparticle delivery systems in the clinic. Challenges in the manufacturing of PLGA nanoparticles is one reason leading to their inability to transition from discovery to clinic. Challenges with conventional methods of manufacturing PLGA nanoparticles Various factors during the manufacturing process can influence the performance of the nanoparticles. Size is one key factor that influences the delivery of PLGA nanoparticles to specific tissues. For example, nanoparticles of sizes below nm exhibited higher uptake into tumor cells when compared to nanoparticles having sizes greater than nm [6]. However, barring a few reports, most conventional methods of manufacturing PLGA nanoparticles are unable to achieve stable sizes below nm [7]. Size is another parameter which can be used by researchers to influence the drug release kinetics and biodistribution of nanoparticles depending on the applications [8]. Although most conventional methods like emulsion solvent diffusion (ESD), Emulsion Solvent Evaporation (ESE), and nanoprecipitation can manufacture PLGA nanoparticles in the range of - nm, they lack the precise control in tuning the size of the nanoparticles [9]. Uniformity in size is another key factor in the manufacture of nanoparticles. Narrow size distributions lead to more consistent results amongst different batches. Most current methods for manufacturing PLGA nanoparticles typically yield broad size distributions and require additional purification steps to obtain narrow distributions. However, these methods lower the yield leading to product losses during manufacturing. Lack of uniformity leads to batch-to-batch variability which is another cause of concern as variability amongst different batches can lead to PLGA nanoparticles showing significantly different efficacies from batch-tobatch, suggesting the need for reproducible manufacturing processes [9]. Although the manufacture of nanoparticles on the lab-scale has been studied extensively, scaling up the manufacturing to larger quantities in many cases is not possible leading to the production of smaller batches and maintaining reproducibility amongst batches. As PLGA nanoparticle size depends on various process parameters, changes in the manufacturing processes during scale-up leads to loss of desired characteristics seen on the lab-scale or heavy optimizations which slows down the transition of the drug to the market. During scale-up of nanoparticle manufacturing, time taken by different manufacturing procedures is important since longer processes on the lab-scale can lead to slow throughput during large scale manufacturing.[1-13]. Can microfluidics be the solution? Microfluidic methods for manufacturing nanoparticles provide precise control over nanoparticle characteristics such as size and distribution at the nanolitre scale through the use of microfluidic mixing cartridges. The NanoAssemblr microfluidic platform is an automated system, which in addition to precise control, removes user variability leading to a high degree of reproducibility between batches. The rapid mixing on the small scale enables high throughput manufacturing of nanoparticles with narrow size distributions. Continuous flow methodology and parallelization of mixers ensure consistent reaction conditions from volumes as low as 1 ml to tens of litres. In this white paper, we report the rapid and controlled manufacturing of PLGA nanoparticles using the NanoAssemblr microfluidic platform. We further describe how the NanoAssemblr technology can address the challenges associated with conventional methods of manufacturing PLGA nanoparticles such as achieving sub- nm size, uniformity, rational design, high throughput, reproducibility, and scalability. We further describe the encapsulation of a hydrophobic model drug Coumarin-6 in PLGA nanoparticles. 1

2 Microfluidic synthesis of PLGA nanoparticles PLGA polymer in acetonitrile and PVA (Polyvinyl alcohol) in water were pumped through two separate inlets of the NanoAssemblr microfluidic cartridge. As the two fluids passed through the staggered herringbone mixing (SHM) structures, they wrapped over each other causing rapid mixing of the two phases. The rapid increase in the polarity of the PLGA polymer solution led to precipitation of the polymer to form PLGA nanoparticles with PVA coating the surface of the nanoparticles as a surfactant. The resulting solution containing PLGA nanoparticles was collected from the outlet and subsequently dialyzed over night against water. The influence of instrument parameters such as total flow rate (TFR) and aqueous:organic flow rate ratio (FRR), and formulation parameters such as polymer and surfactant concentration on nanoparticle size and polydispersity were investigated (Table 1). Use of microfluidics for the encapsulation of hydrophobic small molecule drug Coumarin-6 in PLGA nanoparticles was further investigated. Table 1. Instrument and formulation parameters for the manufacture of PLGA nanoparticles using the NanoAssemblr microfluidic platform. the biodistribution of nanoparticles, small changes in size between batches can alter the efficacy of the nanoparticle formulations from batch-to-batch. Heterogeneous mixing environments in conventional methods of manufacturing often lead to inconsistency amongst batches and higher polydispersity in particle size. Microfluidics, on the other hand, creates a homogenous mixing environment favoring reproducibility and lower polydispersity in particle size [16]. Figure 1 shows the ability of the NanoAssemblr platform to manufacture sub- nm particle reproducibly when manufactured at different times by different users following a set protocol. A B C D E Formulation Sample Polymer Organic phase Stabilizer Aqueous phase PLGA (5:5) ester-terminated (45-55K) PLGA polymer in acetonitrile Poly(vinyl alcohol) mol% hydrolyzed (~31K) PVA in water Instrument Parameters 2-12 ml/min 12 ml/min Figure 1. PLGA nanoparticles of size 75- nm and ~ were reproducibly manufactured using the NanoAssemblr platform at different times and by different users. Aqueous:organic flow rate ratio (FRR) Polymer (PLGA) concentration Stabilizer (PVA) concentration 1:1-9:1 Formulation Parameters 5 - mg/ml.5-4. % w/v Microfluidics enables reproducible manufacture of sub- nm PLGA nanoparticles Two key challenges of many conventional methods of manufacturing PLGA nanoparticles is the inability to obtain particle sizes below nm and maintaining batch-to-batch consistency. Literature reports suggest that while larger size particles tend to be cleared rapidly from the body, smaller size particles (< nm) exhibit reduced clearance and have a greater ability to reach their intended target [14, 15]. Since size is of critical importance in determining Exploiting Instrument parameters to tune the size of PLGA nanoparticles For formulation scientists, the ability to reproducibly tune the size of the nanoparticles is extremely important in the design of a successful nanocarrier. This would help in optimizing the particle size and find the right candidate for scale-up. The NanoAssemblr platform provides two instrument parameters that can be exploited to tune particle size: the total flow rate (TFR) and the aqueous:organic flow rate ratio (FRR). The TFR in ml/min dictates the mixing time and is the total combined speed at which the two fluids are being pumped into the two inlets of the microfluidic device. It is equal to the flow rate of the solution coming out through the outlet channel. Increasing the TFR ensures faster mixing and decreases the mixing time. Figure 2 shows the effect of TFR on the particle size of PLGA nanoparticles. Nanopar- 2

3 ticles at concentration of PLGA showed a minimal decrease in size up to 7 nm indicating that this was the lowest possible size attained for this polymer system under these conditions. Higher concentrations were able to show a larger decrease in the particle size as TFR increased from 2 to 12 ml/min. The higher flow rates ensures faster mixing times of the two phases as compared to the polymer precipitation time which leads to the formation of smaller nanoparticles [17]. A Size (nm) 7 5 or mg/ml 2-12 ml/min Figure 2. Effect of total flow rate (TFR) on the size and of PLGA nanoparticles at polymer concentrations of (A) 5 mg/ml and (B) mg/ml using the NanoAssemblr Benchtop instrument. The aqueous: organic flow rate ratio (FRR) is the ratio of the two phases that are interacting with each other as they get pumped through the microfluidic device. A ratio of 3 or 3:1 means that 3 parts of aqueous solution (PVA in water) is mixied with 1 part of organic solution (PLGA in Acetonitrile). Figure 3 shows the effect of FRR on the size and of PLGA nanoparticles. As the FRR increases from 1:1 to 9:1, the size of the nanoparticles increases from 135 nm to 1 nm. FRR increase leads to a more rapid shift in the polarity and faster precipitation of the polymer. In the case of Figure 2, increased flow rate led to faster mixing time as compared to precipitation time for the polymer which led to smaller nanoparticles. However, at constant TFR, if the FRR increases, the precipitation time of the polymer decreases leading to larger sizes at higher FRRs Size (nm) FRR (Aqueous:organic) B.6 mg/ml 12 ml/min - 9:1 Figure 3. Effect of aqueous:organic flow rate ratio (FRR) on the size and of PLGA nanoparticles at polymer concentrations of mg/ml using the NanoAssemblr Benchtop. Exploiting formulation parameters to tune the size of PLGA nanoparticles Another approach to tune the size of PLGA nanoparticles using the NanoAssemblr is by modifying the formulation parameters such as the concentration of both the polymer (PLGA) as well as the stabilizer (PVA). This gives the formulation scientist additional options to optimize the size of the nanoparticles without changing the molecular weight or composition of lactic and glycolic acid for PLGA, or the type of stabilizer used. Figure 4 shows the effect of PLGA polymer concentration on the size and of PLGA nanoparticles. Increasing the concentration of the PLGA polymer from 5 to mg/ml increased the size of the nanoparticles from 7 nm up to nm. The increase in size of the nanoparticles is similar to results reported in the literature and may be due to the fact that the increased concentration leads to higher viscosity of the solutions that decreases the diffusion rate of the organic phase into the aqueous phase thereby increasing the mixing time and hence increasing particle size [18] PLGA Concentration (mg/ml) mg/ml 8 ml/min Figure 4. Effect of PLGA polymer concentration on the size and of PLGA nanoparticles using the NanoAssemblr Benchtop. 3

4 However, concentrations of may sometimes be too low for use in certain studies with the need to be able to formulate nanoparticles at higher concentrations but at sizes below nm. This was achieved by manufacturing a larger batch of PLGA nanoparticles at using the NanoAssemblr, and then concentrating it to higher concentrations via centrifugal filtration. Figure 5 shows how concentrating a batch of PLGA nanoparticles from to 2 led to the nanoparticles having a size of ~9 nm as opposed to formulating directly at 2, which would exhibit a size of ~17 nm. 5 mg/ml 2 concentrated 8 ml/min Figure 5. Change in particle size by concentrating PLGA nanoparticles prepared using the NanoAssemblr Bench top at to 2. The role of the stabilizer is to stabilize the particles by reducing the interfacial tension between the organic phase containing the PLGA polymer and the aqueous phase. Increasing or decreasing the concentration of the stabilizer can thus have an impact on the size and of PLGA nanoparticles. As seen in Figure 6, increasing the concentration of PVA from.5 to 2% w/v led to a decrease in the size of the nanoparticles. These results are similar to that reported in the literature and is due to the reduction in interfacial tension as concentration increases [18]. No further reduction in particle size was reported at PVA concentrations of 4% w/v which may be because 2% w/v PVA seems enough to efficiently stabilize these nanoparticles. Using several parameters, the NanoAssemblr Benchtop was able to formulate PLGA nanoparticles from as low as 7 nm which is usually not easily attainable by most conventional methods of manufacture up to nm. Concentration of the PLGA polymer can be used to increase or decrease the size of the particles and then the flow rate and flow rate ratio can be further adjusted to fine-tune the nanoparticle size. mg/ml.5-8 ml/min Figure 6. Effect of PVA stabilizer concentration on the size and of PLGA nanoparticles at polymer concentrations of mg/ml using the NanoAssemblr Bench top. Effect of post-processing steps on the size and of PLGA nanoparticles The post-processing steps for removal of acetonitrile can also influence the size and of PLGA nanoparticles. Figure 7 shows the effect of dialysis or centrifugal filtration on the size and of PLGA nanoparticles PVA Concentration (% w/v).5 Dialysis Size CF Size Dialysis CF 2-12 ml/min Figure 7. Effect of post-processing method on the size and of PLGA nanoparticles at polymer concentrations of 5 mg/ml using the NanoAssemblr Benchtop (CF centrifugal filtration). 4

5 Centrifugal filtration leads to a slight reduction in the of the PLGA nanoparticles. One reason can be the speed at which the solvent removal takes place during centrifugal filtration versus dialysis. It is possible that the slow removal of solvent facilitates an uneven amount of particle growth and increase in during the acetonitrile removal process. Encapsulation of hydrophobic drug in PLGA Nanoparticles PLGA nanoparticle are known for the encapsulation and delivery of hydrophobic drugs. However, again, the size of nanoparticles usually seen after encapsulation of hydrophobic drugs in PLGA nanoparticles is above nm. Herein, we have used the NanoAssemblr Benchtop to manufacture PLGA nanoparticles -loaded with a hydrophobic model drug, Coumarin-6. Coumarin-6 is a low molecular weight (MW: 353) fluorescent probe which is insoluble in water but soluble in organic solvents such as ethanol, methanol, N,N-Dimethylformamide, and acetonitrile. The excitation and emission maximum for Coumarin-6 is 45 nm and 55 nm, respectively. Coumarin-6 was successfully encapsulated into PLGA nanoparticles by dissolving Coumarin-6 into the organic phase along with the PLGA polymer and formulating nanoparticles using the NanoAssemblr Bench top with 2 % w/v PVA in water. Figure 8 shows the effect of encapsulation of Coumarin-6 on the size of PLGA nanoparticles. Loading of Coumarin-6 into the PLGA nanoparticle did not seem to significantly change the particle size of the nanoparticles when using either methods of post-processing via dialysis or centrifugal filtration. A B Empty PLGA Nanoparticles Loaded PLGA Nanoparticles 2-12 ml/min Figure 8. Effect of Coumarin-6 encapsulation on the size and of PLGA nanoparticles at polymer concentrations of prepared using the NanoAssemblr Benchtop and post-processed with either (A) dialysis or (B) centrifugal filtration. Empty PLGA Nanoparticles Loaded PLGA Nanoparticles Coumarin-6 showed a maximum encapsulation of 75 % w/w when formulated using the NanoAssemblr Benchtop with an initial drug loading of 5 % w/w of the polymer. This was considerably higher than that reported in the literature for similar PLGA polymer and stabilizer loaded with a similar model drug using a single-emulsion method (~ % w/w).[19]. The size of PLGA nanoparticles prepared using the NanoAssemblr Benchtop was ~ nm as opposed to ~177 nm for that reported in literature [19] indicating the ability of the NanoAssemblr platform to encapsulate more drug at smaller sizes. Both the post-processing method and the total flow rate influenced the final encapsulation efficiency of PLGA nanoparticles prepared using the NanoAssemblr Benchtop. Figure 9 shows the effect of TFR and post-processing method on the encapsulation efficiency of Coumarin-6. Centrifugal filtration led to lower encapsulation efficiencies as compared to dialysis. One possible reason for the lower encapsulation efficiency can be the increased stress placed on PLGA nanoparticles during centrifugal filtration as opposed to dialysis which could lead to lower encapsulations. Increasing the TFR from 4 to 12 ml/min showed an increase in the average encapsulation efficiency from 55 to 73 % w/w. % Encapsulation efficiency 2-12 ml/min Figure 9. Encapsulation of Coumarin-6 (5 % w/w of the polymer) in PLGA nanoparticles at polymer concentrations of prepared using the NanoAssemblr Benchtop and post-processed with either Dialysis or Centrifugal filtration (CF Centrifugal filtration). Conclusion Loaded PLGA NP/Dialysis Loaded PLGA NP/CF TFR (m L/m in) The NanoAssemblr Benchtop can reproducibly achieve sub- nm size PLGA nanoparticles and also rapidly tune 5

6 their size using various instrument and formulation parameters, depending on the required application. The NanoAssemblr also enabled the successful encapsulation of Coumarin-6 in PLGA nanoparticles at amounts higher than that reported in the literature while still maintaining a size below nm. In conclusion, the NanoAssemblr Benchtop forms an important tool in the efficient manufacture of PLGA nanoparticles for the encapsulation and delivery of hydrophobic small-molecule therapeutics. Shyam Garg Formulation Scientist at Precision NanoSystems Inc. Shyam has a degree in Pharmacy from the University of Mumbai in India and a Ph.D. in Pharmaceutical Sciences from the University of Alberta. Prior to working at PNI, Shyam was a researcher at the University of Alberta where he developed polymer based nanocarriers for improving the delivery of cancer therapeutics to their targets. References 1. Jain KK. Nanopharmaceuticals. In: The Handbook of Nanomedicine, (Ed.^(Eds).Humana Press Totowa, NJ (8). 2. Kalepu S, Nekkanti V. Insoluble drug delivery strategies: review of recent advances and business prospects. Acta Pharm Sin B 5(5), (15). 3. Tiwari G, Tiwari R, Sriwastawa B et al. Drug delivery systems: An updated review. Int J Pharm Investig 2(1), 2-11 (12). 4. Farokhzad OC, Langer R. Impact of nanotechnology on drug delivery. ACS Nano 3(1), 16- (9). 5. Lu JM, Wang X, Marin-Muller C et al. Current advances in research and clinical applications of PLGA-based nanotechnology. Expert Rev Mol Diagn 9(4), (9). 6. Choi JS, Cao J, Naeem M et al. Size-controlled biodegradable nanoparticles: preparation and size-dependent cellular uptake and tumor cell growth inhibition. Colloids Surf B Biointerfaces (14). 7. Astete CE, Sabliov CM. Synthesis and characterization of PLGA nanoparticles. J Biomater Sci Polym Ed 17(3), (6). 8. Mahapatro A, Singh DK. Biodegradable nanoparticles are excellent vehicle for site directed in-vivo delivery of drugs and vaccines. J Nanobiotechnology 9 55 (11). 9. Xie H, Smith JW. Fabrication of PLGA nanoparticles with a fluidic nanoprecipitation system. J Nanobiotechnology 8 18 (1). 1. Paliwal R, Babu RJ, Palakurthi S. Nanomedicine scale-up technologies: feasibilities and challenges. AAPS PharmSciTech 15(6), (14). 11. Desai N. Challenges in development of nanoparticle-based therapeutics. AAPS J 14(2), (12). 12. Vauthier C, Bouchemal K. Processing and Scale-up of Polymeric Nanoparticles. In: Intracellular Delivery: Fundamentals and Applications, Prokop A (Ed.^(Eds).Springer Netherlands Dordrecht (11). 13. Anselmo AC, Mitragotri S. Nanoparticles in the clinic. Bioengineering & Translational Medicine n/a-n/a (16). 14. Gaumet M, Vargas A, Gurny R, Delie F. Nanoparticles for drug delivery: the need for precision in reporting particle size parameters. Eur J Pharm Biopharm 69(1), 1-9 (8). 15. Alexis F, Pridgen E, Molnar LK, Farokhzad OC. Factors affecting the clearance and biodistribution of polymeric nanoparticles. Mol Pharm 5(4), (8). 16. Garg SM, Heuck G, Ip S, Ramsay E. Microfluidics: a transformational tool for nanomedicine development and production. Journal of Drug Targeting In Press 17. Johnson BK, Prud, Homme RK. Flash NanoPrecipitation of Organic Actives and Block Copolymers using a Confined Impinging Jets Mixer. Australian Journal of Chemistry 56(1), (3). 18. Halayqa M, Domanska U. PLGA biodegradable nanoparticles containing perphenazine or chlorpromazine hydrochloride: effect of formulation and release. Int J Mol Sci 15(12), (14). 19. Kulkarni SA, Feng SS. Effects of surface modification on delivery efficiency of biodegradable nanoparticles across the blood-brain barrier. Nanomedicine (Lond) 6(2), (11). 6

Continuous Microfluidic Synthesis of PLGA Nanoparticles by Micromixing

Continuous Microfluidic Synthesis of PLGA Nanoparticles by Micromixing Continuous Microfluidic Synthesis of PLGA Nanoparticles by Micromixing Dolomite s Nanoparticle Generation System Application Note Page Summary 2 Polymer Nanoparticles 3 Mechanism Micromixing Solvent Diffusion

More information

Continuous Microfluidic Synthesis of PLGA Microparticles by Droplet Method

Continuous Microfluidic Synthesis of PLGA Microparticles by Droplet Method Microfluidic Synthesis PLGA Microparticles by Droplet Method - App. Note Continuous Microfluidic Synthesis of PLGA Microparticles by Droplet Method Dolomite s API encapsulation system for PLGA 20 µm to

More information

Continuous Synthesis of Monodisperse PLGA Particles using Droplets

Continuous Synthesis of Monodisperse PLGA Particles using Droplets Continuous Synthesis of Monodisperse PLGA Particles using Droplets Droplet system for controlled and reproducible encapsulation of API in uniform polymer beads Version 1.2 21/04/2017 Pavel Abdulkin and

More information

NANO 243/CENG 207 Course Use Only

NANO 243/CENG 207 Course Use Only L12: Drug Loading & Quantification May 15, 2018 1. Drug loading techniques 1.1 Physical approaches Nanprecipitation Single emulsion Double emulsion Encapsulation Remote loading 1.2 Chemical approaches

More information

EXPERIMENTAL CONTRIBUTIONS IN THE SYNTHESIS OF PLGA NANOPARTICLES WITH EXCELLENT PROPERTIES FOR DRUG DELIVERY: INVESTIGATION OF KEY PARAMETERS

EXPERIMENTAL CONTRIBUTIONS IN THE SYNTHESIS OF PLGA NANOPARTICLES WITH EXCELLENT PROPERTIES FOR DRUG DELIVERY: INVESTIGATION OF KEY PARAMETERS U.P.B. Sci. Bull., Series B, Vol. 79, Iss. 2, 2017 ISSN 1454-2331 EXPERIMENTAL CONTRIBUTIONS IN THE SYNTHESIS OF PLGA NANOPARTICLES WITH EXCELLENT PROPERTIES FOR DRUG DELIVERY: INVESTIGATION OF KEY PARAMETERS

More information

ELECTROSPRAY: NOVEL FABRICATION METHOD FOR BIODEGRADABLE POLYMERIC NANOPARTICLES FOR FURTHER APPLICATIONS IN DRUG DELIVERY SYSTEMS

ELECTROSPRAY: NOVEL FABRICATION METHOD FOR BIODEGRADABLE POLYMERIC NANOPARTICLES FOR FURTHER APPLICATIONS IN DRUG DELIVERY SYSTEMS ELECTROSPRAY: NOVEL FABRICATION METHOD FOR BIODEGRADABLE POLYMERIC NANOPARTICLES FOR FURTHER APPLICATIONS IN DRUG DELIVERY SYSTEMS Ali Zarrabi a, Manouchehr Vossoughi b a Institute for Nanscience & Nanotechnology,

More information

PRODUCTION OF DRUG NANOPARTICLES OF CONTROLLABLE SIZE USING SUPERCRITICAL FLUID ANTISOLVENT TECHNIQUE WITH ENHANCED MASS TRANSFER

PRODUCTION OF DRUG NANOPARTICLES OF CONTROLLABLE SIZE USING SUPERCRITICAL FLUID ANTISOLVENT TECHNIQUE WITH ENHANCED MASS TRANSFER PRODUCTION OF DRUG NANOPARTICLES OF CONTROLLABLE SIZE USING SUPERCRITICAL FLUID ANTISOLVENT TECHNIQUE WITH ENHANCED MASS TRANSFER Gupta R.B 1, and Chattopadhyay P.* 2 1-Auburn University, 2-Ferro Corporation.

More information

Simulation of mixing and precipitation of nanoparticles for pharmaceutical applications with CFD and MD

Simulation of mixing and precipitation of nanoparticles for pharmaceutical applications with CFD and MD 14th European Conference on Mixing Warszawa, 10-13 September 2012 Simulation of mixing and precipitation of nanoparticles for pharmaceutical applications with CFD and MD N. Di Pasquale 1, P. Carbone 2,

More information

Novel Drug Delivery Systems II Preparation of Nanoparticles

Novel Drug Delivery Systems II Preparation of Nanoparticles Paper No. : 08 Novel Drug Delivery Systems Module No 15: Development Team Principal Investigator Prof. Farhan J Ahmad Jamia Hamdard, New Delhi Paper Coordinator Dr. Sushama Talegaonkar Jamia Hamdard, New

More information

Encapsulation. Battelle Technology. Introduction

Encapsulation. Battelle Technology. Introduction Encapsulation Introduction The encapsulation methods reported in the literature 1-7 for the production of microcapsules are generally achieved using one of the following techniques: 1. Phase separation

More information

MACRO RESEARCH: FABRICATION OF

MACRO RESEARCH: FABRICATION OF MACRO RESEARCH: FABRICATION OF NANOPARTICLES FOR DRUG DELIVERY Cristina Sabliov, Associate Professor Biological and Agricultural Engineering November 19, 29 NANOPARTICLES A. H. FARAJI, P. WIPF. 29. NANOPARTICLES

More information

Preparation of PLGA nanoparticles using TPGS in the spontaneous emulsification solvent diffusion method

Preparation of PLGA nanoparticles using TPGS in the spontaneous emulsification solvent diffusion method Journal of Experimental Nanoscience ISSN: 1745-8080 (Print) 1745-8099 (Online) Journal homepage: http://www.tandfonline.com/loi/tjen20 Preparation of PLGA nanoparticles using TPGS in the spontaneous emulsification

More information

L Department of Mechanical Engineering in Partial Fulfillment of the Requirements for the Degree of

L Department of Mechanical Engineering in Partial Fulfillment of the Requirements for the Degree of Characterization of Mixing in a Coaxial Jet Mixer for Nanoparticle Fabrication by Laura Gilson ARCHNES M.I.T. LIBRARIES OCT 2 2013 Submitted to the L Department of Mechanical Engineering in Partial Fulfillment

More information

D'Addio, SM; Chan, JG; Kwok, P; Prud'homme, R; Chan, HK

D'Addio, SM; Chan, JG; Kwok, P; Prud'homme, R; Chan, HK Title Spray freeze dried large porous particles for nano drug delivery by inhalation Author(s) D'Addio, SM; Chan, JG; Kwok, P; Prud'homme, R; Chan, HK Citation The 2012 Respiratory Drug Delivery (RDD)

More information

Poly (Lactic Co-Glycolic) Acid Nanoparticles: Synthesis Using Millifluidic Chip and Interaction with Red Blood Cells

Poly (Lactic Co-Glycolic) Acid Nanoparticles: Synthesis Using Millifluidic Chip and Interaction with Red Blood Cells Louisiana State University LSU Digital Commons LSU Master's Theses Graduate School 2015 Poly (Lactic Co-Glycolic) Acid Nanoparticles: Synthesis Using Millifluidic Chip and Interaction with Red Blood Cells

More information

Capsule Construction Investigations Using Polyvinyl alcohol and Vegetable oil

Capsule Construction Investigations Using Polyvinyl alcohol and Vegetable oil Capsule Construction Investigations Using Polyvinyl alcohol and Vegetable oil Figure 1 Images taken Figure 2 Image Figure 3 Image with through a light with Red Oil O Red Oil O and microscope ocular 400x.

More information

COMPUTATIONAL APPROACH FOR PARTICLE SIZE MEASUREMENT OF SILVER NANOPARTICLE FROM ELECTRON MICROSCOPIC IMAGE

COMPUTATIONAL APPROACH FOR PARTICLE SIZE MEASUREMENT OF SILVER NANOPARTICLE FROM ELECTRON MICROSCOPIC IMAGE Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 09-9 Vol, Suppl, 0 COMPUTATIONAL APPROACH FOR PARTICLE SIZE MEASUREMENT OF SILVER NANOPARTICLE FROM ELECTRON MICROSCOPIC

More information

Simulation of a 3D Flow-Focusing Capillary-Based Droplet Generator

Simulation of a 3D Flow-Focusing Capillary-Based Droplet Generator Simulation of a 3D Flow-Focusing Capillary-Based Droplet Generator D. Conchouso*, E. Rawashdeh, A. Arevalo, D. Castro, I. G. Foulds King Abdullah University of Science and Technology 4700 KAUST 23955,

More information

Meet Stunner: The one-shot protein concentration and sizing combo

Meet Stunner: The one-shot protein concentration and sizing combo TECH NOTE Meet Stunner: The one-shot protein concentration and sizing combo Introduction What if you could get a better read on the quality of your biologics and use less sample at the same time? Stunner

More information

Research strategy for Micro and complex fluids

Research strategy for Micro and complex fluids Research strategy for Micro and complex fluids Introduction Presently there is a strong trend towards miniaturizing equipment for chemical analysis and synthesis. This is made possible by development of

More information

Supplementary Information

Supplementary Information Supplementary Information A Microfluidic Platform to design crosslinked Hyaluronic Acid Nanoparticles (chanps) for enhanced MRI Maria Russo a,b, Paolo Bevilacqua a,c, Paolo Antonio Netti a,b,d and Enza

More information

Preparation of Cefuroxime Loaded PVP Particles by Supercritical Anti-Solvent Process

Preparation of Cefuroxime Loaded PVP Particles by Supercritical Anti-Solvent Process Preparation of Cefuroxime Loaded PVP Particles by Supercritical Anti-Solvent Process I. N. Uzun 1, N. Baran Acaralı 1, S. Deniz 1, O. Sipahigil 2, S. Dinçer 1*. 1 Yildiz Technical University, Department

More information

SURFACE-MODIFIED BIODEGRADABLE NANOPARTICLES FOR TARGETED DRUG DELIVERY

SURFACE-MODIFIED BIODEGRADABLE NANOPARTICLES FOR TARGETED DRUG DELIVERY SURFACE-MODIFIED BIODEGRADABLE NANOPARTICLES FOR TARGETED DRUG DELIVERY SEDYAKINA Nataliya E. 1, GORSHKOVA Marina Y. 2, MAKSIMENKO Olga O. 1, GELPERINA Svetlana E. 1 1 Nanosystem LTD., Moscow, Russian

More information

CDER Risk Assessment to Evaluate Potential Risks from the Use of Nanomaterials in Drug Products

CDER Risk Assessment to Evaluate Potential Risks from the Use of Nanomaterials in Drug Products CDER Risk Assessment to Evaluate Potential Risks from the Use of Nanomaterials in Drug Products Celia N. Cruz, Ph.D. CDER Nanotechnology Working Group Office of Pharmaceutical Science 1 Disclaimer The

More information

Data requirements for reactor selection. Professor John H Atherton

Data requirements for reactor selection. Professor John H Atherton Data requirements for reactor selection Professor John H Atherton What is my best option for manufacturing scale? Microreactor Mesoscale tubular reactor Tubular reactor OBR (oscillatory baffled flow reactor)

More information

Detonation Nanodiamond Suspensions

Detonation Nanodiamond Suspensions PRODUCT SHEET Rev. 10/17, v3.0 Detonation Nanodiamond Suspensions Colloidal suspensions of detonation nanodiamond (DND) in both and a variety of organic solvents have a wide range of uses, including: (1)

More information

Potassium ion-recognizable responsive smart materials

Potassium ion-recognizable responsive smart materials Potassium ion-recognizable responsive smart materials *Xiao-Jie Ju 1), Zhuang Liu 2), Hai-Rong Yu 2), Rui Xie 1), Wei Wang 3) and Liang-Yin Chu 4) 1), 2), 3), 4) School of Chemical Engineering, Sichuan

More information

Preparation of poly(dl-lactide-co-glycolide) nanoparticles by modified spontaneous emulsification solvent diffusion method

Preparation of poly(dl-lactide-co-glycolide) nanoparticles by modified spontaneous emulsification solvent diffusion method International Journal of Pharmaceutics 187 (1999) 143 152 www.elsevier.com/locate/promis Preparation of poly(dl-lactide-co-glycolide) nanoparticles by modified spontaneous emulsification solvent diffusion

More information

The Better Way to Characterize Nanoparticles MANTA s ViewSizer 3000

The Better Way to Characterize Nanoparticles MANTA s ViewSizer 3000 info@mantainc.com www.mantainc.com The Better Way to Characterize Nanoparticles MANTA s ViewSizer 3000 MANTA s Most Advanced Nanoparticle Tracking Analysis technology performs individual particle analysis

More information

10/18/2006. Biotage A Life Science Technology Company

10/18/2006. Biotage A Life Science Technology Company 10/18/2006 Biotage A Life Science Technology Company About Biotage Headquartered in Uppsala, Sweden Three Centers of Excellence Uppsala, Sweden Research & Development Charlottesville VA, U.S.A. Instrument

More information

Gold Nanoparticle Conjugated PLGA-PEG-SA-PEG-PLGA Multiblock Copolymer Nanoparticles: Synthesis, Characterization, In-vivo Release of Rifampicin

Gold Nanoparticle Conjugated PLGA-PEG-SA-PEG-PLGA Multiblock Copolymer Nanoparticles: Synthesis, Characterization, In-vivo Release of Rifampicin Gold Nanoparticle Conjugated PLGA-PEG-SA-PEG-PLGA Multiblock Copolymer Nanoparticles: Synthesis, Characterization, In-vivo Release of Rifampicin Mani Gajendiran, a Sheik Mohammed Jainuddin Yousuf, b Vellaichamy

More information

Model based design of controlled release drug delivery systems

Model based design of controlled release drug delivery systems Model based design of controlled release drug delivery systems Aditya Pareek, Praduman Arora, Venkataramana Runkana Copyright 2012 Tata Consultancy Services Limited 1 Why we need controlled drug delivery?

More information

Emulsion Processing - Homogenization -

Emulsion Processing - Homogenization - Emulsion Processing - Homogenization - Jochen Weiss *Food Structure and Functionality Laboratories Department of Food Science & Biotechnology University of Hohenheim Garbenstrasse 21, 70599 Stuttgart,

More information

Chapter 6 Magnetic nanoparticles

Chapter 6 Magnetic nanoparticles Chapter 6 Magnetic nanoparticles Magnetic nanoparticles (MNPs) are a class of nanoparticle which can be manipulated using magnetic field gradients. Such particles commonly consist of magnetic elements

More information

Services in Chemistry for a Sustainable World

Services in Chemistry for a Sustainable World Table of Content 2 Services in Chemistry for a Sustainable World COMPANY PROFILE TECHNICAL PROFILE General Information... 3 Our Services... 4 Project Types & Pricing Options... 5 Reaction & Catalyst Portfolio...

More information

Visualize and Measure Nanoparticle Size and Concentration

Visualize and Measure Nanoparticle Size and Concentration NTA : Nanoparticle Tracking Analysis Visualize and Measure Nanoparticle Size and Concentration 30 Apr 2015 NanoSight product range LM 10 series NS300 series NS500 series Dec 13 34 www.nanosight.com NanoSight

More information

Preparation of Polycaprolactone Nanoparticles via Supercritical Carbon-dioxide Extraction Of Emulsions.

Preparation of Polycaprolactone Nanoparticles via Supercritical Carbon-dioxide Extraction Of Emulsions. Preparation of Polycaprolactone Nanoparticles via Supercritical Carbon-dioxide Extraction Of Emulsions. Adejumoke Lara Ajiboye* a, Vivek Trivedi a, *, and John Mitchell a a University of Greenwich, Central

More information

PRODUCTION OF PEG SUBMICRON PARTICLES BY THE SOLUTION ENHANCED DISPERSION WITH ENHANCED MASS TRANSFER BY ULTRASOUND IN SUPERCRITICAL CO 2 (SEDS-EM)

PRODUCTION OF PEG SUBMICRON PARTICLES BY THE SOLUTION ENHANCED DISPERSION WITH ENHANCED MASS TRANSFER BY ULTRASOUND IN SUPERCRITICAL CO 2 (SEDS-EM) PRODUCTION OF PEG SUBMICRON PARTICLES BY THE SOLUTION ENHANCED DISPERSION WITH ENHANCED MASS TRANSFER BY ULTRASOUND IN SUPERCRITICAL CO 2 (SEDS-EM) Heyang Jin, Sining Li, Daode Hu and Yaping Zhao* Email

More information

Rapid Preparation of Polymersomes by a Water Addition/Solvent Evaporation Method. Supporting Information

Rapid Preparation of Polymersomes by a Water Addition/Solvent Evaporation Method. Supporting Information Rapid Preparation of Polymersomes by a Water Addition/Solvent Evaporation Method Supporting Information Hana Robson Marsden, Luca Gabrielli, Alexander Kros* Department of Soft Matter Chemistry, Leiden

More information

Physicochemical Characterization of Acyclovir Topical Semisolid Dosage Forms Towards TCS Validation Flavian Ștefan Rădulescu, Dalia Simona Miron

Physicochemical Characterization of Acyclovir Topical Semisolid Dosage Forms Towards TCS Validation Flavian Ștefan Rădulescu, Dalia Simona Miron Physicochemical Characterization of Acyclovir Topical Semisolid Dosage Forms Towards TCS Validation Flavian Ștefan Rădulescu, Dalia Simona Miron University of Medicine and Pharmacy Carol Davila, Bucharest,

More information

Combinatorial Heterogeneous Catalysis

Combinatorial Heterogeneous Catalysis Combinatorial Heterogeneous Catalysis 650 μm by 650 μm, spaced 100 μm apart Identification of a new blue photoluminescent (PL) composite material, Gd 3 Ga 5 O 12 /SiO 2 Science 13 March 1998: Vol. 279

More information

Quick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE

Quick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE Quick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE Introduction Gel permeation chromatography (GPC) and size exclusion chromatography

More information

Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX

Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX Technical Note Agilent s SampliQ WAX provides Applications for strongly acidic, acidic and neutral compounds Excellent

More information

8. FORMULATION OF LANSOPRAZOLE NANOPARTICLES

8. FORMULATION OF LANSOPRAZOLE NANOPARTICLES 8. FORMULATION OF LANSOPRAZOLE NANOPARTICLES FORMULATION OF LANSOPRAZOLE NANOPARTICLES Preparation of capsule of modified solubility to protect the drug from degradation To protect the drug from degradation

More information

Lack of correlation of polymer-drug dispersion stability with Hildebrand or Hansen Solubility Parameters

Lack of correlation of polymer-drug dispersion stability with Hildebrand or Hansen Solubility Parameters HSP50 York, 6 th April 2017 Lack of correlation of polymer-drug dispersion stability with Hildebrand or Hansen Solubility Parameters M.C.Garnett 1, E. Turpin 1, V. Taresco 1, C.A.Laughton 1, J. Burley

More information

Modulation of Poly(β-amino ester) ph-sensitive Polymers by Molecular Weight Control

Modulation of Poly(β-amino ester) ph-sensitive Polymers by Molecular Weight Control Macromolecular Research, Vol. 13, No. 2, pp 147-151 (2005) Modulation of Poly(β-amino ester) ph-sensitive Polymers by Molecular Weight Control Min Sang Kim and Doo Sung Lee* Department of Polymer Science

More information

Formation of Alginate-Membrane Capsules by using Co-Extrusion Dripping Technique

Formation of Alginate-Membrane Capsules by using Co-Extrusion Dripping Technique Formation of Alginate-Membrane Capsules by using Co-Extrusion Dripping Technique E.S. Chan*, W.O. Hong, B.B. Lee, Z.H. Yim, P. Ravindra Centre of Materials & Minerals, School of Engineering and Information

More information

Applying the Taguchi Method to Optimise the Size of Silica Nanoparticles Entrapped with Rifampicin for a Drug Delivery System

Applying the Taguchi Method to Optimise the Size of Silica Nanoparticles Entrapped with Rifampicin for a Drug Delivery System Journal of Engineering Science, Vol. 11, 9 16, 2015 Applying the Taguchi Method to Optimise the Size of Silica Nanoparticles Entrapped with Rifampicin for a Drug Delivery System Nor Ain Zainal, 1 Syamsul

More information

Polymeric nanoparticles containing diazepam: preparation, optimization, characterization, in-vitro drug release and release kinetic study

Polymeric nanoparticles containing diazepam: preparation, optimization, characterization, in-vitro drug release and release kinetic study Bohrey et al. Nano Convergence (2016) 3:3 DOI 10.1186/s40580-016-0061-2 Open Access RESEARCH Polymeric nanoparticles containing diazepam: preparation, optimization, characterization, in-vitro drug release

More information

Stable Encapsulation of Quantum Dot Barcodes with Silica Shells

Stable Encapsulation of Quantum Dot Barcodes with Silica Shells Stable Encapsulation of Quantum Dot Barcodes with Silica Shells Shang-Hsiu Hu and Xiaohu Gao Department of Bioengineering, University of Washington Seattle, WA 98195 (USA) Adv. Funct. Mater. 2010. ASAP

More information

Flow Focusing Droplet Generation Using Linear Vibration

Flow Focusing Droplet Generation Using Linear Vibration Flow Focusing Droplet Generation Using Linear Vibration A. Salari, C. Dalton Department of Electrical & Computer Engineering, University of Calgary, Calgary, AB, Canada Abstract: Flow focusing microchannels

More information

Gel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC)

Gel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC) Gel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC) Size Exclusion Chromatography (SEC) is a non-interaction based separation mechanism in which compounds are retained for different

More information

Hydrogel Biomaterials: Structure and thermodynamics

Hydrogel Biomaterials: Structure and thermodynamics Hydrogel Biomaterials: Structure and thermodynamics Last Day: programmed/regulated/multifactor controlled release for drug delivery and tissue eng ineering Announcements: Today: Reading: Finish discussion

More information

The microparticle system has become an indispensable part of the controlled drug delivery fields for the past few decades since it can

The microparticle system has become an indispensable part of the controlled drug delivery fields for the past few decades since it can Microencapsulation Microencapsulation is a process or technique by which thin coatings can be applied reproducibly to small particles of solids, droplets of liquids, or dispersions, thus, forming microcapsules.

More information

The solution for all of your

The solution for all of your The solution for all of your nanoparticle sizing and zeta potential needs. DelsaNano Series Blood Banking Capillary Electrophoresis Cell Analysis Centrifugation Genomics Lab Automation Lab Tools Particle

More information

European Journal of Pharmaceutical Sciences

European Journal of Pharmaceutical Sciences European Journal of Pharmaceutical Sciences 41 (2010) 244 253 Contents lists available at ScienceDirect European Journal of Pharmaceutical Sciences journal homepage: www.elsevier.com/locate/ejps Preparation

More information

CHEMICAL ENGINEERING (CHE)

CHEMICAL ENGINEERING (CHE) Chemical Engineering (CHE) 1 CHEMICAL ENGINEERING (CHE) CHE 2033 Introduction to Chemical Process Engineering Prerequisites: CHEM 1515 and ENSC 2213 Description: Concurrent enrollment in MATH 2233 or 3263,

More information

Supercritical Antisolvent Precipitation of Sotalol Hydrochloride: Influence of Solvent and of Apparatus Design

Supercritical Antisolvent Precipitation of Sotalol Hydrochloride: Influence of Solvent and of Apparatus Design Supercritical Antisolvent Precipitation of Sotalol Hydrochloride: Influence of Solvent and of Apparatus Design P.Alessi*, I.Kikic, F. Vecchione T.Gamse (1) Dipartimento di Ingegneria Chimica, dell Ambiente

More information

How proteins separate on reverse-phase HPLC

How proteins separate on reverse-phase HPLC 1 Reverse Phase How proteins separate on reverse-phase HPLC RP chromatography separates proteins through the interaction of the hydrophobic foot of the protein with a nonpolar surface of the particle RP

More information

Opportunities and Implications for. the 21st Century

Opportunities and Implications for. the 21st Century Opportunities and Implications for Green Nanotechnology in Industry for the 21st Century Reducing Principles to Practice June 16, 2010 John M. Miller, Ph.D. Dune Sciences, Inc. Nanotechnology CLEAN WATER

More information

Scholars Research Library. Formulation, Optimization and characterization of Simvastatin Nanosuspension prepared by nanoprecipitation technique

Scholars Research Library. Formulation, Optimization and characterization of Simvastatin Nanosuspension prepared by nanoprecipitation technique Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2011, 3(2): 129-140 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Current developments and applications of microfluidic. technology toward clinical translation of nanomedicines

Current developments and applications of microfluidic. technology toward clinical translation of nanomedicines Current developments and applications of microfluidic technology toward clinical translation of nanomedicines Dongfei Liu a,b,c *, Hongbo Zhang c,d,e, Flavia Fontana a, Jouni T. Hirvonen a, and Hélder

More information

Sustainable Formation of Curcumin Nanoparticle: Stirred Tank and Confined Impinging Jet Reactor

Sustainable Formation of Curcumin Nanoparticle: Stirred Tank and Confined Impinging Jet Reactor Sustainable Formation of Curcumin Nanoparticle: Stirred Tank and Confined Impinging Jet Reactor Yue Yang and Kunn Hadinoto Ong Nanyang Technological University, Singapore Email: yyang3@e.ntu.edu.sg, kunnong@ntu.edu.sg

More information

Understanding Surfactants and New Methods of Dispersing

Understanding Surfactants and New Methods of Dispersing Understanding Surfactants and New Methods of Dispersing Chemists and process engineers far and wide find that their job is commonly a neverending rush to what could be made better. Ideas on how to control

More information

Dicyclomine-loaded Eudragit -based Microsponge with Potential for Colonic Delivery: Preparation and Characterization

Dicyclomine-loaded Eudragit -based Microsponge with Potential for Colonic Delivery: Preparation and Characterization Tropical Journal of Pharmaceutical esearch, February 2010; 9 (1): 67-72 Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. esearch Article

More information

INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES

INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES Research Article An In-Vitro Evaluation for the Effect of Β-Cyclodextrin and PVP-K 3 on Drug Release Pattern of Sertraline Hydrochloride Deepa Warrier 1, Aanna Zagade 1, Amir Shaikh 2*, Yogesh Pawar 2

More information

Sample Preparation. Approaches to Automation for SPE

Sample Preparation. Approaches to Automation for SPE Sample Preparation Approaches to Automation for SPE i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this reference

More information

Biomedical Approach of Nanomaterials for Drug Delivery

Biomedical Approach of Nanomaterials for Drug Delivery International Journal of Chemistry and Chemical Engineering. ISSN 2248-9924 Volume 3, Number 2 (2013), pp. 95-100 Research India Publications http://www.ripublication.com Biomedical Approach of Nanomaterials

More information

Supramolecular DNA nanotechnology. Faisal A. Aldaye

Supramolecular DNA nanotechnology. Faisal A. Aldaye Supramolecular DA nanotechnology Faisal A. Aldaye Department of Chemistry, McGill University Current address: Department of Systems Biology, Harvard University 200 Longwood Avenue, Boston, MA 02115, USA

More information

IRON OXIDE NANOPARTICLES FOR BIOMEDICAL APPLICATIONS

IRON OXIDE NANOPARTICLES FOR BIOMEDICAL APPLICATIONS Research Paper ISSN 2278 0149 www.ijmerr.com Vol. 3, No. 2, April, 2014 2014 IJMERR. All Rights Reserved IRON OXIDE NANOPARTICLES FOR BIOMEDICAL APPLICATIONS Aadarsh Mishra* *Corresponding Author: Aadarsh

More information

Chemical Engineering Seminar Series

Chemical Engineering Seminar Series Effect of Reaction Conditions on Copolymer Properties Loretta Idowu Keywords: copolymer composition distribution; radical polymerization kinetics; semi-batch starved feed; hydroxyl-functionality Non-functional

More information

Peptide Isolation Using the Prep 150 LC System

Peptide Isolation Using the Prep 150 LC System Jo-Ann M. Jablonski and Andrew J. Aubin Waters Corporation, Milford, MA, USA APPLICATION BENEFITS The Prep 150 LC System, an affordable, highly reliable system for preparative chromatography, is suitable

More information

Basic Principles for Purification Using Supercritical Fluid Chromatography

Basic Principles for Purification Using Supercritical Fluid Chromatography Basic Principles for Purification Using Supercritical Fluid Chromatography Jo-Ann M. Jablonski, Christopher J. Hudalla, Kenneth J. Fountain, Steven M. Collier, and Damian Morrison Waters Corporation, Milford,

More information

Particle Analysis at the Touch of a Button. Litesizer series

Particle Analysis at the Touch of a Button. Litesizer series Particle Analysis at the Touch of a Button Litesizer series Particle systems can be complex The size and stability of nanoparticles and microparticles are crucial to their function, as well as to their

More information

Model-based optimization of polystyrene properties by Nitroxide Mediated Polymerization (NMP) in homogeneous and dispersed media

Model-based optimization of polystyrene properties by Nitroxide Mediated Polymerization (NMP) in homogeneous and dispersed media 1 Model-based optimization of polystyrene properties by Nitroxide Mediated Polymerization (NMP) in homogeneous and dispersed media Lien Bentein NMP: principle and objective Nitroxide mediated polymerization

More information

next generation Welcome to the Dispersion options Smarter particle sizing Your sample, expertly processed and prepared Particle size

next generation Welcome to the Dispersion options Smarter particle sizing Your sample, expertly processed and prepared Particle size Particle size Welcome to the next generation Your sample, expertly processed and prepared Dispersion options 3000 detailed specification sheets from Getting your sample preparation right The US Pharmacopeia

More information

Determination of Solubility Parameters using Inverse Gas Chromatography

Determination of Solubility Parameters using Inverse Gas Chromatography Determination of Solubility Parameters using Inverse Gas Chromatography Anett Kondor PhD IGC-SEA Product Manager and igc Product Specialist 7 th April 017 Overview Introduction Inverse Gas Chromatography

More information

295 J App Pharm 03(03): (2011) Nayak et al., 2011 COMPARATIVE STABILITY STUDY OF METRONIDAZOLE IN AQUEOUS AND NON AQUEOUS VEHICLE

295 J App Pharm 03(03): (2011) Nayak et al., 2011 COMPARATIVE STABILITY STUDY OF METRONIDAZOLE IN AQUEOUS AND NON AQUEOUS VEHICLE 295 J App Pharm 03(03): 295-300 (2011) Nayak et al., 2011 Research Article COMPARATIVE STABILITY STUDY OF METRONIDAZOLE IN AQUEOUS AND NON AQUEOUS VEHICLE Satish Nayak, D. C. Goupale*, Atul Dubey and Vipin

More information

Co-precipitation of Curcumin-PVP via the Supercritical Antisolvent Process

Co-precipitation of Curcumin-PVP via the Supercritical Antisolvent Process OD08 Co-precipitation of Curcumin-PVP via the Supercritical Antisolvent Process Ravenna Lessa Matos a, *, Tiejun Lu a, Valentina Prosapio a, Christopher McConville b, Gary Leeke c, Andrew Ingram a a Centre

More information

CHEM 429 / 529 Chemical Separation Techniques

CHEM 429 / 529 Chemical Separation Techniques CHEM 429 / 529 Chemical Separation Techniques Robert E. Synovec, Professor Department of Chemistry University of Washington Lecture 1 Course Introduction Goal Chromatography and Related Techniques Obtain

More information

Multi-Channel SFC System for Fast Chiral Method Development and Optimization

Multi-Channel SFC System for Fast Chiral Method Development and Optimization Multi-Channel SFC System for Fast Chiral Method Development and ptimization Lakshmi Subbarao, Ziqiang Wang, Ph.D., and Rui Chen, Ph.D. Waters Corporation, Milford, USA APPLICATIN BENEFITS The Method Station

More information

Chemistry 3200 High Performance Liquid Chromatography: Quantitative Determination of Headache Tablets

Chemistry 3200 High Performance Liquid Chromatography: Quantitative Determination of Headache Tablets Chemistry 3200 High Performance Liquid Chromatography: Quantitative Determination of Headache Tablets Liquid chromatography was developed by Tswett in early 1900 s and was shown to be a powerful separation

More information

Phase Behaviour of Microemulsion Systems Containing Tween-80 and Brij-35 as Surfactant

Phase Behaviour of Microemulsion Systems Containing Tween-80 and Brij-35 as Surfactant Received on 20/04/2012; Revised on 29/04/2012; Accepted on 09/06/2012 Phase Behaviour of Microemulsion Systems Containing Tween-80 and Brij-35 as Surfactant Chetan Singh Chauhan *, Navneet singh Chouhan,

More information

CHARACTERISATION OF NANOPARTICLE THROUGH SEM, FTIR, XRD & DSC

CHARACTERISATION OF NANOPARTICLE THROUGH SEM, FTIR, XRD & DSC Indian Institute of Technology Kharagpur From the SelectedWorks of Ajit Behera 2011 CHARACTERISATION OF NANOPARTICLE THROUGH SEM, FTIR, XRD & DSC Ajit Behera, Indian Institute of Technology - Kharagpur

More information

Luke Chimuka. School of Chemistry, University of Witwatersrand

Luke Chimuka. School of Chemistry, University of Witwatersrand Preparation, characterization and application of NaHCO 3 leached bulk U(VI) imprinted polymers endowed with γ-mps coated magnetite in contaminated water Luke Chimuka School of Chemistry, University of

More information

Setting Attainable and Practical Particle Size Specifications

Setting Attainable and Practical Particle Size Specifications Setting Attainable and Practical Particle Size Specifications Mark Bumiller HORIBA Scientific mark.bumiller@horiba.com Why Set Specifications? Product release (quality) Communicate grade to buyers Internal

More information

Impact factor: /ICV: PROCESS VALIDATION OF PARACETAMOL SUSPENSION Sisal Shah*, Dinesh G. Desai, A. K.

Impact factor: /ICV: PROCESS VALIDATION OF PARACETAMOL SUSPENSION Sisal Shah*, Dinesh G. Desai, A. K. Impact factor:.3397/icv: 4. 299 Pharma Science Monitor 6(2), Apr-Jun 25 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com PROCESS VALIDATION

More information

Using Design of Experiments to Optimize Chiral Separation

Using Design of Experiments to Optimize Chiral Separation 1 of 7 10/5/2010 1:15 PM Home About us Guidance e-newsletter Expert Insights Readers' Choice Resources Audio & Video» QbD News Categorized Expert Insights, Resources Using Design of Experiments to Optimize

More information

Modelling of drug release from ensembles of aspirin microcapsules of certain particle size distribution

Modelling of drug release from ensembles of aspirin microcapsules of certain particle size distribution Eichie & Okor, 23 Tropical Journal of Pharmaceutical Research, June 23; 2 (1): 137-145 Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria. All rights reserved. Available

More information

Continuous, efficient multistage extraction

Continuous, efficient multistage extraction Continuous, efficient multistage extraction Andrea Adamo Zaiput Flow Technologies Department of Chemical Engineering, MIT Munich 01 February 2017 Back ground - MIT s efforts in continuous manufacturing

More information

Mesoporous Silica Nanoparticles as Complex Bioactive Delivery Vehicles

Mesoporous Silica Nanoparticles as Complex Bioactive Delivery Vehicles Mesoporous Silica Nanoparticles as Complex Bioactive Delivery Vehicles Kathleen Eggleson, Ph.D. Center for Nano Science and Technology University of Notre Dame Characteristics of mesoporous materials Material

More information

PRODUCTION OF L-PLA MICROPARTICLES BELOW AND ABOVE THE MIXTURE CRITICAL PRESSURE OF THE SYSTEM DCM-CO 2

PRODUCTION OF L-PLA MICROPARTICLES BELOW AND ABOVE THE MIXTURE CRITICAL PRESSURE OF THE SYSTEM DCM-CO 2 PRODUCTION OF L-PLA MICROPARTICLES BELOW AND ABOVE THE MIXTURE CRITICAL PRESSURE OF THE SYSTEM DCM-CO 2 Y. Pérez * (a), H. Pellikaan (b), F. E. Wubbolts (a), G. J. Witkamp (a), P. J. Jansens (a) (a) Laboratory

More information

Inkjet Printed Reaction Arrays on Pigment Coated Substrates

Inkjet Printed Reaction Arrays on Pigment Coated Substrates Inkjet Printed Reaction Arrays on Pigment Coated Substrates 42 nd iarigai research conference, Helsinki, 07.09.2015 Risto Koivunen 1, Eveliina Jutila 1, Roger Bollström 2, Patrick Gane 1,2 1 Aalto University,

More information

Carbon nanotube coated snowman-like particles and their electro-responsive characteristics. Ke Zhang, Ying Dan Liu and Hyoung Jin Choi

Carbon nanotube coated snowman-like particles and their electro-responsive characteristics. Ke Zhang, Ying Dan Liu and Hyoung Jin Choi Supporting Information: Carbon nanotube coated snowman-like particles and their electro-responsive characteristics Ke Zhang, Ying Dan Liu and Hyoung Jin Choi Experimental Section 1.1 Materials The MWNT

More information

Formulation and Characterization of Asenapine Maleate Nanoparticles

Formulation and Characterization of Asenapine Maleate Nanoparticles Research Article Formulation and Characterization of Asenapine Maleate Nanoparticles Appanna Chowdary K*, Navya Lakshmi Raju Suravarapu, Swathi Meddala St. Ann s College of Pharmacy, Andhra University,

More information

Preparation and characterization of antidiabetic drug loaded polymeric nanoparticles

Preparation and characterization of antidiabetic drug loaded polymeric nanoparticles Available online at www.derpharmachemica.com Scholars Research Library Der Pharma Chemica, 2015, 7(12):398-404 (http://derpharmachemica.com/archive.html) ISSN 09-413X CODEN (USA): PCHHAX Preparation and

More information

MICROSTRUCTURE-BASED PROCESS ENGINEERING AND CATALYSIS

MICROSTRUCTURE-BASED PROCESS ENGINEERING AND CATALYSIS MICROSTRUCTURE-BASED PROCESS ENGINEERING AND CATALYSIS 1 2 APPLICATION PORTFOLIO Fine chemistry We design chemical processes from lab to pilot scale in a safe, efficient and flexible way: using micro-

More information

Monodisperse Microspheres for Parenteral Drug Delivery

Monodisperse Microspheres for Parenteral Drug Delivery PARENTERAL D E L I V E R Y Monodisperse Microspheres for Parenteral Drug Delivery By: Gert Veldhuis, PhD, Míriam Gironès, PhD, MSc and Debra Bingham INTRODUCTION Throughout the past few years, several

More information

POLYMER STABILIZED NANOSUPENSIONS VIA FLASH NANOPRECIPITATION: PARTICLE FORMULATION, STRUCTURE AND FREEZE DRYING

POLYMER STABILIZED NANOSUPENSIONS VIA FLASH NANOPRECIPITATION: PARTICLE FORMULATION, STRUCTURE AND FREEZE DRYING POLYMER STABILIZED NANOSUPENSIONS VIA FLASH NANOPRECIPITATION: PARTICLE FORMULATION, STRUCTURE AND FREEZE DRYING A DISSERTATION SUBMITTED TO THE FACULTY OF THE GRADUATE SCHOOL OF THE UNIVERSITY OF MINNESOTA

More information

Supplementary Information

Supplementary Information Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2015 Supplementary Information Visualization of equilibrium position of colloidal particles at fluid-water

More information