Encapsulation of biological materials

Size: px
Start display at page:

Download "Encapsulation of biological materials"

Transcription

1 Engineering Conferences International ECI Digital Archives Design and Manufacture of Functional Microcapsules and Engineered Products Proceedings Encapsulation of biological materials Alexander Routh University of Cambridge, Follow this and additional works at: Recommended Citation Alexander Routh, "Encapsulation of biological materials" in "Design and Manufacture of Functional Microcapsules and Engineered Products", Chair: Simon Biggs, University of Queensland (Aus) Co-Chairs: Olivier Cayre, University of Leeds, UK Orlin D. Velev, North Carolina State University, USA Eds, ECI Symposium Series, (2016). This Abstract and Presentation is brought to you for free and open access by the Proceedings at ECI Digital Archives. It has been accepted for inclusion in Design and Manufacture of Functional Microcapsules and Engineered Products by an authorized administrator of ECI Digital Archives. For more information, please contact

2 Encapsulation for Biological Material Alex Routh Dept of Chemical Engineering and Biotechnology BP Institute University of Cambridge 1

3 About the BP Institute Formed in 1999 with an endowment from BP 6 academics in 5 Cambridge departments Prof Andy Woods Earth Sciences Dr Stuart Clarke Chemistry Dr Jie Li Engineering Dr Colm Caulfield DAMTP Dr Jerome Neufeld Earth Sciences/DAMTP Dr Alex Routh Chemical Engineering 2

4 Outline Colloidosomes How to make them Release profiles Biological encapsulation Yeast Lactic acid bacteria Enzymes Release by Shear Dilution Acid Metal shells Targeted delivery 3

5 Encapsulation: Colloidosome Formation Continuous phase (organic) Emulsification 1 Shell stabilisation by sintering 2 Transfer & redispersion Dispersed phase (aqueous) Solvent miscible with interior fluid Bacteria Latex particles Surfactant 4

6 Optical Microscopy Water-in-oil microcapsules Dried colloidosome microcapsules - scale ~ 10µm - - scale ~ 10µm - 5

7 SEM Colloidosome microcapsules - scale ~ 10µm - Coagulated latex particles forming single particle layer shell - scale ~ 200nm - Langmuir 25(1):

8 Effect of Sintering Time 5 min 30 min 60 min - scale for all images ~ 500nm - These colloidosomes have been sintered as shown while maintaining a constant sintering temperature of 7 C above the glass transition temperature (Tg) of the polymer. Langmuir 25(1):

9 Colloidosomes dye release experiment Relese rate seems to be invariant to sintering time 100 Release Fraction (%) min 30 min 60 min Time (mins) Langmuir 25(1):

10 Colloidosomes for biological encapsulation Colloidosomes are poor for encapsulation of small molecules e.g perfumes in washing powders want an inorganic shell for this Colloidosomes are inherently porous with holes around the size of the base colloidal particles Like a string shopping bag Ideal for biological encapsulation Nutrients can freely diffuse in and out Simple biologically friendly production self assembly production 9

11 Yeast in colloidosomes Fluorescence & transmission confocal microscopy images of a colloidosome encapsulating viable Baker s Yeast cells stained with FUN 1 10

12 Yeast in colloidosomes Time series of optical microscope images showing yeast cells moving and dividing within a colloidosome Langmuir, 28(2):

13 Glucose trends Glucoseconcentration / mmol Time / hrs Colloidosomes encapsulating yeast (single latex stage) Water-core colloidosomes Unencapsulated yeast Unencapsulated yeast exposed to 0.25% Virkon for 10 min Colloidosomes encapsulating yeast (double latex stage) Langmuir, 28(2):

14 Lactic acid bacteria in colloidosomes Encapsulation of lactic acid bacteria Maintain viability through simulated gastric and intestinal conditions Probiotic yoghurts Health effects dependent on survival of probiotic bacteria through stomach 13

15 Avoiding killing or damaging the bacteria Ethanol causes cell membrane damage Need to alter method of aggregating colloidal latex particles Salt causes colloidal aggregation Lactic acid bacteria have a relatively high tolerance to salt Success with a combination of NaCl and Ethanol 14

16 Encapsulated viable Lactic Acid Bacteria Green fluorescence = viable undamaged bacteria Transmission and confocal microscopy images of colloidosomes encapsulating Lactic acid bacteria stained with fluorescent molecular probes 15

17 Encapsulated Lactic Acid Bacteria: Glucose and ph trends Langmuir 28(46):

18 Encapsulated Lactic Acid Bacteria: Protection at low ph Langmuir 28(46):

19 Enzymes in Colloidosomes We want Survival for months in washing detergent Ease of release on dilution and shear Added to washing liquids to break down fats Enzymes are denatured by the surfactants Does encapsulation provide protection? Release upon Acid Shear Dilution Coating with CaCO 3 to provide absolute barrier 18

20 Unsealed colloidosomes will leak Enzymes are smaller than bacteria they release from standard colloidosomes Unsealed colloidosomes will release amylase enzyme over 30 hours Langmuir 30: United Kingdom Patent Application No

21 Production of colloidosomes with inorganic shell Simple to make with inorganic shells add Na 2 CO 3 and CaCl 2 to the two aqueous phases Langmuir 30: United Kingdom Patent Application No

22 SEM images No CaCO3 25% CaCO3 40% CaCO3 40% CaCO3 21

23 Release with acid With a dye: release on application of acid 25% encapsulation yield Langmuir 30: United Kingdom Patent Application No

24 Final release upon application of shear With a dye: increase in CaCO 3 in shell needs larger shear to cause release Increasing shear Langmuir 30: United Kingdom Patent Application No

25 Triggered release on shear or dilution Enzyme can be released by shear Enzyme can be released by dilution Langmuir 30: United Kingdom Patent Application No

26 Sealed colloidosomes protect enzyme Release activity upon shear Unencapsulated enzymes and unsealed colloidosomes display reduced activity Langmuir 30: United Kingdom Patent Application No

27 Aqueous core colloidosomes with a metal shell Dye release % Silver Coated Colloidosomes: AgNO3 in wash solution, reducing agent in core a b 100 Blank sample Polymer shell 50 c d Release silver shell Silver shell Time/h Optical microscope image, SEM images and TEM image of silver shell colloidosomes: (a) optical microscope image; (b and c) SEM images; and (d) TEM image (1)dye release % (dye) (2)dye release % (dye+latex) (3)dye release % (dye+latex+silver) (4)dye release % (polymer shell colloidosomes) (5)dye release % (silver shell colloidosomes) (6)dye release % (triggered silver shell colloidosomes) Release of Allura Red dye from colloidosomes sealed with a silver shell, polymer shell and unsealed 26

28 Aqueous core colloidosomes with a metal shell SEM images of spherical silver shell colloidosomes SEM images of gold coated colloidosomes Thiol chemistry (including SH structure) Tumor Colloidosome One potential application of metal coated colloidosomes - targeted drug delivery 27

29 Conclusions Colloidosomes are a biologically benign method of encapsulation Range of biological material can be encapsulated shell locking step requires species specific method Continued biological activity is observed Metal shell can be placed around colloidosome Colloidosomes can be targeted to specific proteins 28

30 Acknowledgements Grace Yow Polly Keen Qian Sun 29

Controlled adsorption of metallic nanoparticles on polymeric microcapsules with a view to growing secondary continuous metal films

Controlled adsorption of metallic nanoparticles on polymeric microcapsules with a view to growing secondary continuous metal films Engineering Conferences International ECI Digital Archives Design and Manufacture of Functional Microcapsules and Engineered Products Proceedings 4-7-2016 Controlled adsorption of metallic nanoparticles

More information

Encapsulation. Loughborough University Institutional Repository

Encapsulation. Loughborough University Institutional Repository Loughborough University Institutional Repository Encapsulation This item was submitted to Loughborough University's Institutional Repository by the/an author. Citation: VLADISAVLJEVIC, G.T. and HOLDICH,

More information

Exploiting the Interaction of Pyranine-3 with Poly(L-Lysine) to Mediate Nanoparticle Assembly: Fabrication of Dynamic ph-responsive Nanocontainers

Exploiting the Interaction of Pyranine-3 with Poly(L-Lysine) to Mediate Nanoparticle Assembly: Fabrication of Dynamic ph-responsive Nanocontainers Exploiting the Interaction of Pyranine-3 with Poly(L-Lysine) to Mediate Nanoparticle Assembly: Fabrication of Dynamic ph-responsive Nanocontainers Arlin Jose Amali, a Shashi Singh, b Nandini Rangaraj,

More information

Encapsulation. Battelle Technology. Introduction

Encapsulation. Battelle Technology. Introduction Encapsulation Introduction The encapsulation methods reported in the literature 1-7 for the production of microcapsules are generally achieved using one of the following techniques: 1. Phase separation

More information

3 rd Food Emulsions Short Course November 13 th & 14 th, 2008 University of Massachusetts. David Julian McClements University of Massachusetts

3 rd Food Emulsions Short Course November 13 th & 14 th, 2008 University of Massachusetts. David Julian McClements University of Massachusetts 3 rd Food Emulsions Short Course November 13 th & 14 th, 2008 University of Massachusetts David Julian McClements University of Massachusetts Program Objectives Review Basic Principles of Emulsion Science

More information

Exploring how new formulation technologies could improve the properties of natural colours

Exploring how new formulation technologies could improve the properties of natural colours Natural Colour Summit 2013 Exploring how new formulation technologies could improve the properties of natural colours Dr Jim Bullock iformulate Ltd jim@iformulate.biz +44 7450 436515 A new company (2012)

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION In the format provided by the authors and unedited. Phagocytosis- inspired behaviour in synthetic protocell communities of compartmentalized colloidal objects Laura Rodríguez- Arco, Mei Li and Stephen

More information

Supplementary Information

Supplementary Information Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2015 Supplementary Information Visualization of equilibrium position of colloidal particles at fluid-water

More information

Food safety and nanotechnology: Results from LENA Project. Richard Winterhalter

Food safety and nanotechnology: Results from LENA Project. Richard Winterhalter Food safety and nanotechnology: Results from LENA Project Richard Winterhalter Outline Project LENA Nanoparticles in food and food supplements Colloidal silver Field-Flow-Fractionation Measurement of nanosilver

More information

NANO 243/CENG 207 Course Use Only

NANO 243/CENG 207 Course Use Only L12: Drug Loading & Quantification May 15, 2018 1. Drug loading techniques 1.1 Physical approaches Nanprecipitation Single emulsion Double emulsion Encapsulation Remote loading 1.2 Chemical approaches

More information

Highly Sensitive and Selective Colorimetric Visualization of Streptomycin in Raw Milk Using Au Nanoparticles Supramolecular Assembly

Highly Sensitive and Selective Colorimetric Visualization of Streptomycin in Raw Milk Using Au Nanoparticles Supramolecular Assembly SUPPORTING INFORMATION Highly Sensitive and Selective Colorimetric Visualization of Streptomycin in Raw Milk Using Au Nanoparticles Supramolecular Assembly Jiayu Sun, Jiechao Ge, Weimin Liu, Zhiyuan Fan,

More information

BIJEL CAPSULES. Institute for Condensed Matter and Complex Systems and The Edinburgh Complex Fluid Partnership

BIJEL CAPSULES. Institute for Condensed Matter and Complex Systems and The Edinburgh Complex Fluid Partnership BIJEL CAPSULES J.W. Tavacoli, E.M. Herzig, and P.S Clegg Institute for Condensed Matter and Complex Systems and The Edinburgh Complex Fluid Partnership School of Physics, University of Edinburgh Bijel

More information

A Simple and Green Method for Synthesis of Ag and Au Nanoparticles Using Biopolymers and Sugars as Reducing Agents.

A Simple and Green Method for Synthesis of Ag and Au Nanoparticles Using Biopolymers and Sugars as Reducing Agents. A Simple and Green Method for Synthesis of Ag and Au Nanoparticles Using Biopolymers and Sugars as Reducing Agents. German Ayala, Luci Cristina O. Vercik, Leticcia G. Ferreira, Andres Vercik and Thiago

More information

Size and Zeta Potential of Colloidal Gold Particles

Size and Zeta Potential of Colloidal Gold Particles Size and Zeta Potential of Colloidal Gold Particles Mark Bumiller mark.bumiller@horiba.com Colloid Definition Two phases: Dispersed phase (particles) Continuous phase (dispersion medium, solvent) May be

More information

2 Preparation of hollow spheres, microcapsules and microballoons by surfactant free emulsion templating

2 Preparation of hollow spheres, microcapsules and microballoons by surfactant free emulsion templating 2 Preparation of hollow spheres, microcapsules and microballoons by surfactant free emulsion templating We report on the synthesis of new types of monodisperse, micrometer-sized hollow particles obtained

More information

TEST BANK FOR PRESCOTTS MICROBIOLOGY 9TH EDITION BY WILLEY SHERWOOD WOOLVERTON

TEST BANK FOR PRESCOTTS MICROBIOLOGY 9TH EDITION BY WILLEY SHERWOOD WOOLVERTON TEST BANK FOR PRESCOTTS MICROBIOLOGY 9TH EDITION BY WILLEY SHERWOOD WOOLVERTON Link download full: https://testbankservice.com/download/test-bank-for-prescottsmicrobiology-9th-edition-by-willey-sherwood-woolverton/

More information

An introduction to particle size characterisation by DCS:

An introduction to particle size characterisation by DCS: An introduction to particle size characterisation by DCS: Do you know the real size of your nano particles? By Dr Hiran Vegad, Analytik Ltd Introduction Differential centrifugal sedimentation (DCS) is

More information

Monolayers. Factors affecting the adsorption from solution. Adsorption of amphiphilic molecules on solid support

Monolayers. Factors affecting the adsorption from solution. Adsorption of amphiphilic molecules on solid support Monolayers Adsorption as process Adsorption of gases on solids Adsorption of solutions on solids Factors affecting the adsorption from solution Adsorption of amphiphilic molecules on solid support Adsorption

More information

ph-triggered aggregation of responsive polymer-stabilized colloids and the reversible formation of porous scaffolds

ph-triggered aggregation of responsive polymer-stabilized colloids and the reversible formation of porous scaffolds Supporting Information: ph-triggered aggregation of responsive polymer-stabilized colloids and the reversible formation of porous scaffolds Robert T. Woodward, Christopher Hight, Ufuk Yildiz, Nicolas Schaeffer,

More information

Design of Pickering Emulsion Templated Colloidosomes and Fundamentals of Emulsification with Nanoparticles

Design of Pickering Emulsion Templated Colloidosomes and Fundamentals of Emulsification with Nanoparticles IPST Members Meeting Design of Pickering Emulsion Templated Colloidosomes and Fundamentals of Emulsification with Nanoparticles Hongzhi Wang Sven Research Group April 10,2012 Research Overview Dual research

More information

Protein separation and characterization

Protein separation and characterization Address:800 S Wineville Avenue, Ontario, CA 91761,USA Website:www.aladdin-e.com Email USA: tech@aladdin-e.com Email EU: eutech@aladdin-e.com Email Asia Pacific: cntech@aladdin-e.com Protein separation

More information

*blood and bones contain colloids. *milk is a good example of a colloidal dispersion.

*blood and bones contain colloids. *milk is a good example of a colloidal dispersion. Chap. 3. Colloids 3.1. Introduction - Simple definition of a colloid: a macroscopically heterogeneous system where one component has dimensions in between molecules and macroscopic particles like sand

More information

ELECTRONIC SUPPLMENTARY INFORMATION (ESI) Doubly crosslinked microgel-colloidosomes: a versatile

ELECTRONIC SUPPLMENTARY INFORMATION (ESI) Doubly crosslinked microgel-colloidosomes: a versatile Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 1 ELECTRONIC SUPPLMENTARY INFORMATION (ESI) Doubly crosslinked microgel-colloidosomes: a versatile

More information

Contents. Preface XIII

Contents. Preface XIII V Contents Preface XIII 1 General Introduction 1 1.1 Fundamental Knowledge Required for Successful Dispersion of Powders into Liquids 1 1.1.1 Wetting of Powder into Liquid 1 1.1.2 Breaking of Aggregates

More information

CHAPTER 7: Solutions & Colloids 7.2 SOLUBILITY. Degrees of Solution. Page PHYSICAL STATES of SOLUTIONS SOLUTION

CHAPTER 7: Solutions & Colloids 7.2 SOLUBILITY. Degrees of Solution. Page PHYSICAL STATES of SOLUTIONS SOLUTION CHAPTER 7: Solutions & Colloids Predict the relative solubility of materials on the basis of polarity Describe solution formation in terms of solutesolvent interactions Calculate solution concentrations

More information

Nanoparticle Technology. Dispersions in liquids: suspensions, emulsions, and foams ACS National Meeting April 9 10, 2008 New Orleans

Nanoparticle Technology. Dispersions in liquids: suspensions, emulsions, and foams ACS National Meeting April 9 10, 2008 New Orleans Nanoparticle Technology Dispersions in liquids: suspensions, emulsions, and foams ACS National Meeting April 9 10, 2008 New Orleans Wetting in nano ACS National Meeting April 9 10, 2008 New Orleans 10

More information

8. FORMULATION OF LANSOPRAZOLE NANOPARTICLES

8. FORMULATION OF LANSOPRAZOLE NANOPARTICLES 8. FORMULATION OF LANSOPRAZOLE NANOPARTICLES FORMULATION OF LANSOPRAZOLE NANOPARTICLES Preparation of capsule of modified solubility to protect the drug from degradation To protect the drug from degradation

More information

the universal solvent

the universal solvent Chapter 7: Acids, Bases, and Solutions Solution a homogeneous mixture Solutions have the same properties throughout, containing solute particles (molecules or ions) that are too small to see Solvent the

More information

Particle Size Analysis with Differential Centrifugal Sedimentation. Marc Steinmetz Sales & Support Manager CPS Instruments Europe

Particle Size Analysis with Differential Centrifugal Sedimentation. Marc Steinmetz Sales & Support Manager CPS Instruments Europe Particle Size Analysis with Differential Centrifugal Sedimentation Marc Steinmetz Sales & Support Manager CPS Instruments Europe What is important to you from a particle sizing technique? Many people talk

More information

Supporting Information

Supporting Information Supporting Information Dual nanocomposite multihollow polymer microspheres prepared by suspension polymerization based on a multiple Pickering emulsion Quanxing Gao, Chaoyang Wang,* Hongxia Liu, Xinxing

More information

Controlled Assembly of Organic Whispering Gallery Mode Microlasers as Highly Sensitive Chemical Sensors

Controlled Assembly of Organic Whispering Gallery Mode Microlasers as Highly Sensitive Chemical Sensors Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Electronic Supplementary Information Controlled Assembly of Organic Whispering Gallery Mode Microlasers

More information

SYNTHESIS AND PROCESSING OF METALLIC NANOMATERIALS USING CO 2 EXPANDED LIQUIDS AS A GREEN SOLVENT MEDIUM

SYNTHESIS AND PROCESSING OF METALLIC NANOMATERIALS USING CO 2 EXPANDED LIQUIDS AS A GREEN SOLVENT MEDIUM SYNTHESIS AND PROCESSING OF METALLIC NANOMATERIALS USING CO 2 EXPANDED LIQUIDS AS A GREEN SOLVENT MEDIUM Christopher Kitchens Dept. of Chemical and Biomolecular Engineering Clemson University, SC ENGINEERED

More information

COLLOID CHEMISTRY MD. KHAIRUL ISLAM

COLLOID CHEMISTRY MD. KHAIRUL ISLAM COLLOID CHEMISTRY MD. KHAIRUL ISLAM HISTORICAL BACKGROUND Thomas Graham (1861) observed that crystalline substances such as sugar, urea, and sodium chloride passed through the membrane, while others like

More information

Magnetic Silica Particles for Catalysis

Magnetic Silica Particles for Catalysis 4 Magnetic Silica Particles for atalysis Abstract Monodisperse magnetizable colloidal silica particles in a stable dispersion have been functionalized with a homogeneous catalyst: a PP-pincer Pd-complex.

More information

Interaction of Gold Nanoparticle with Proteins

Interaction of Gold Nanoparticle with Proteins Chapter 7 Interaction of Gold Nanoparticle with Proteins 7.1. Introduction The interfacing of nanoparticle with biomolecules such as protein is useful for applications ranging from nano-biotechnology (molecular

More information

Supplementary Information

Supplementary Information Supplementary Information Metal tips on pyramid-shaped PbSe/CdSe/CdS heterostructure nanocrystal photocatalysts: study of ripening and core/shell formation Whi Dong Kim, a Sooho Lee, a Chaewon Pak, a Ju

More information

A Two-step Synthesis for Preparing Metal Microcapsules with a Biodegradable Polymer Substrate

A Two-step Synthesis for Preparing Metal Microcapsules with a Biodegradable Polymer Substrate Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is The Royal Society of Chemistry 2018 A Two-step Synthesis for Preparing Metal Microcapsules with a Biodegradable

More information

Fabrication of ordered array at a nanoscopic level: context

Fabrication of ordered array at a nanoscopic level: context Fabrication of ordered array at a nanoscopic level: context Top-down method Bottom-up method Classical lithography techniques Fast processes Size limitations it ti E-beam techniques Small sizes Slow processes

More information

Pre-seeding -assisted synthesis of high performance polyamide-zeolite nanocomposie membrane for water purification

Pre-seeding -assisted synthesis of high performance polyamide-zeolite nanocomposie membrane for water purification Electronic Supporting Information: Pre-seeding -assisted synthesis of high performance polyamide-zeolite nanocomposie membrane for water purification Chunlong Kong, a Takuji Shintani b and Toshinori Tsuru*

More information

Self-assembled Peptides as Drug Delivery Molecules for transport across the Biological Barriers

Self-assembled Peptides as Drug Delivery Molecules for transport across the Biological Barriers Self-assembled Peptides as Drug Delivery Molecules for transport across the Biological Barriers Porter, S., McCarthy, H., & Laverty, G. (2017). Self-assembled Peptides as Drug Delivery Molecules for transport

More information

Encapsulation technologies:

Encapsulation technologies: Encapsulation technologies: A general overview Capsulae Presentation outline Introduction Definition of encapsulation Objectives and markets Encapsulation technologies Dripping Spray-drying Prilling Coating

More information

Core-shell 2 mesoporous nanocarriers for metal-enhanced fluorescence

Core-shell 2 mesoporous nanocarriers for metal-enhanced fluorescence Core-shell Ag@SiO 2 @msio 2 mesoporous nanocarriers for metal-enhanced fluorescence Jianping Yang a, Fan Zhang a *, Yiran Chen a, Sheng Qian a, Pan Hu a, Wei Li a, Yonghui Deng a, Yin Fang a, Lu Han a,

More information

Plasma functionalization of nano- and micro-sized particles

Plasma functionalization of nano- and micro-sized particles Engineering Conferences International ECI Digital Archives Design and Manufacture of Functional Microcapsules and Engineered Products Proceedings 4-4-2016 Plasma functionalization of nano- and micro-sized

More information

Microstructured Porous Silica Obtained via Colloidal Crystal Templates

Microstructured Porous Silica Obtained via Colloidal Crystal Templates Paper No. 203e Microstructured Porous Silica Obtained via Colloidal Crystal Templates O. D. Velev, T. A. Jede, R. F. Lobo and A. M. Lenhoff Department of Chemical Engineering, University of Delaware, Newark

More information

Microparticle Based Assays

Microparticle Based Assays Microparticle Based Assays Last Class: 1. Mass Transport : Advection Diffusion Equation 2. Boundary Phenomena 3. Physical Properties as a Function of Concentration 4. Mixing/Separation/Purification of

More information

Safety Services. Guidance on the Selection and Use of Disinfectants

Safety Services. Guidance on the Selection and Use of Disinfectants Safety Services Guidance on the Selection and Use of Disinfectants Guidance on the Selection and Use of Disinfectants Chemical disinfectants reduce the number of viable micro-organisms to a level below

More information

A soft-templated method to synthesize sintering-resistant Au/mesoporous-silica core-shell nanocatalysts with sub-5 nm single-core

A soft-templated method to synthesize sintering-resistant Au/mesoporous-silica core-shell nanocatalysts with sub-5 nm single-core A soft-templated method to synthesize sintering-resistant Au/mesoporous-silica core-shell nanocatalysts with sub-5 nm single-core Chunzheng Wu, ab Zi-Yian Lim, a Chen Zhou, a Wei Guo Wang, a Shenghu Zhou,

More information

Adsorption of Methylene Blue on Mesoporous SBA 15 in Ethanol water Solution with Different Proportions

Adsorption of Methylene Blue on Mesoporous SBA 15 in Ethanol water Solution with Different Proportions 2015 2 nd International Conference on Material Engineering and Application (ICMEA 2015) ISBN: 978-1-60595-323-6 Adsorption of Methylene Blue on Mesoporous SBA 15 in Ethanol water Solution with Different

More information

Pickering emulsion engineering: Fabrication of materials with multiple cavities

Pickering emulsion engineering: Fabrication of materials with multiple cavities Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 014 Electronic Supplementary Infomaton Pickering emulsion engineering: Fabrication of materials

More information

One-pot synthesis of micron partly hollow anisotropic dumbbell shaped silica core-shell particles

One-pot synthesis of micron partly hollow anisotropic dumbbell shaped silica core-shell particles Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2016 Supporting Information One-pot synthesis of micron partly hollow anisotropic dumbbell shaped silica

More information

Optimal molecular design of poly (ionic liquids) for CO2 capture from the atmosphere

Optimal molecular design of poly (ionic liquids) for CO2 capture from the atmosphere Engineering Conferences International ECI Digital Archives CO2 Summit II: Technologies and Opportunities Proceedings Spring 4-13-2016 Optimal molecular design of poly (ionic liquids) for CO2 capture from

More information

What is the role of the nucleus? What is the role of the cytoplasm? What is the role of the mitochondria? What is the role of the cell wall. membrane?

What is the role of the nucleus? What is the role of the cytoplasm? What is the role of the mitochondria? What is the role of the cell wall. membrane? Page 1 What is the role of the nucleus? What is the role of the cytoplasm? What is the role of the cell membrane? What is the role of the mitochondria? What is the role of ribosomes? What is the role of

More information

Supporting Information

Supporting Information Supporting Information Precisely Controllable Core-Shell Ag@Carbon Dots Nanoparticles: Application to in Situ Super-Sensitive Monitoring of Catalytic Reactions Jing Jin, Shoujun Zhu, Yubin Song, Hongyue

More information

Supporting Information

Supporting Information Supporting Information Fabrication of Cubic Nanocages and Nanoframes by Dealloying Au/Ag Alloy Nanoboxes with an Aqueous Etchant Based on Fe(NO 3 ) 3 or NH 4 OH Xianmao Lu, 1,2 Leslie Au, 1 Joseph McLellan,

More information

P8D137. Features. Applications. Power UV LED Series is designed for high current operation and high flux output applications. Super high Flux output

P8D137. Features. Applications. Power UV LED Series is designed for high current operation and high flux output applications. Super high Flux output P8D137 Power UV LED Series is designed for high current operation and high flux output applications. P8D137 Features Super high Flux output and high Luminance Furthermore, its thermal management characteristic

More information

P8D1 P8D1. Features. Applications. Power UV LED Series is designed for high current operation and high flux output applications.

P8D1 P8D1. Features. Applications. Power UV LED Series is designed for high current operation and high flux output applications. P8D1 Power UV LED Series is designed for high current operation and high flux output applications. P8D1 Features Super high Flux output and high Luminance Furthermore, its thermal management characteristic

More information

Introduction to Nanoparticle Tracking Analysis (NTA) Measurement Principle of ZetaView

Introduction to Nanoparticle Tracking Analysis (NTA) Measurement Principle of ZetaView Technical Note Nanoparticle Tracking Key words: Introduction to Nanoparticle Tracking Analysis (NTA) Measurement Principle of ZetaView Particle characterization, Nanoparticle Tracking Analysis (NTA), Brownian

More information

Different Biodegradable Silica Structures In Drug Delivery. Mika Jokinen

Different Biodegradable Silica Structures In Drug Delivery. Mika Jokinen Different Biodegradable Silica Structures In Drug Delivery Mika Jokinen & Different Morphologies of Biodegradable Silica - Several levels of morphology ; different forms & structures - monoliths, fibers,

More information

Enhanced Photonic Properties of Thin Opaline Films as a Consequence of Embedded Nanoparticles.

Enhanced Photonic Properties of Thin Opaline Films as a Consequence of Embedded Nanoparticles. Enhanced Photonic Properties of Thin Opaline Films as a Consequence of Embedded Nanoparticles. D E Whitehead, M Bardosova and M E Pemble Tyndall National Institute, University College Cork Ireland Introduction:

More information

Bacterial Morphology and Structure م.م رنا مشعل

Bacterial Morphology and Structure م.م رنا مشعل Bacterial Morphology and Structure م.م رنا مشعل SIZE OF BACTERIA Unit for measurement : Micron or micrometer, μm: 1μm=10-3 mm Size: Varies with kinds of bacteria, and also related to their age and external

More information

Center for Cell Imaging Department of Cell Biology

Center for Cell Imaging Department of Cell Biology Center for Cell Imaging Department of Cell Biology Contents Preparation of Colloidal Gold Conjugates Coupling the Protein A to the Gold Particles Purification of the protein A-gold. Storage Influence of

More information

CELL STRUCTURE & FUNCTION

CELL STRUCTURE & FUNCTION CELL STRUCTURE & FUNCTION CELL TYPES Living cells can be classified into 2 different types on the basis of their internal structure: 4. Prokaryotic Cells 5. Eukaryotic Cells 1. Prokaryotic Cells Are the

More information

Multifunctional polyphosphazene-coated multi-walled carbon. nanotubes for the synergistic treatment of redox-responsive

Multifunctional polyphosphazene-coated multi-walled carbon. nanotubes for the synergistic treatment of redox-responsive Electronic Supplementary Material (ESI) for Polymer Chemistry. This journal is The Royal Society of Chemistry 2017 Supporting information for Multifunctional polyphosphazene-coated multi-walled carbon

More information

SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question.

SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question. Exam Name SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question. Figure 2.1 Using Figure 2.1, match the following: 1) Lipid. 2) Functional protein. 3) Nucleotide.

More information

6 Assembly of microcellular structures from colloidal particles prepared by emulsion templating

6 Assembly of microcellular structures from colloidal particles prepared by emulsion templating 6 Assembly of microcellular structures from colloidal particles prepared by emulsion templating In this chapter we study the materials that can be fabricated by the assembly of hollow shells prepared by

More information

PARTICLE SIZE ANALYSIS OF GOLD NANOPARTICLES

PARTICLE SIZE ANALYSIS OF GOLD NANOPARTICLES PARTICLE SIZE ANALYSIS OF GOLD NANOPARTICLES Scientific interest in well dispersed suspensions of colloidal gold (or nanoparticles) can be traced back to ancient times. Original uses of colloidal gold

More information

26.542: COLLOIDAL NANOSCIENCE & NANOSCALE ENGINEERING Fall 2013

26.542: COLLOIDAL NANOSCIENCE & NANOSCALE ENGINEERING Fall 2013 26.542: COLLOIDAL NANOSCIENCE & NANOSCALE ENGINEERING Fall 2013 Classes: Thurs, 6-9 pm; Ball Hall Room 208 Professor: Dr. B. Budhlall Office: Ball Hall 203B, Phone: 978-934-3414 Email: Bridgette_Budhlall@uml.edu

More information

Stable Encapsulation of Quantum Dot Barcodes with Silica Shells

Stable Encapsulation of Quantum Dot Barcodes with Silica Shells Stable Encapsulation of Quantum Dot Barcodes with Silica Shells Shang-Hsiu Hu and Xiaohu Gao Department of Bioengineering, University of Washington Seattle, WA 98195 (USA) Adv. Funct. Mater. 2010. ASAP

More information

Technology White Papers nr. 10 Paul Holister Cristina Román Vas Tim Harper

Technology White Papers nr. 10 Paul Holister Cristina Román Vas Tim Harper Technology White Papers nr. 10 Paul Holister Cristina Román Vas Tim Harper NANOCAPSULES Technology White Papers nr. 10 Release Date: Published by Científica Científica, Ltd. www.cientifica.com Científica

More information

SUPPORTING INFORMATION

SUPPORTING INFORMATION Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2017 SUPPORTING INFORMATION Synthesis of Circular and Triangular Gold Nanorings with

More information

Preparation of Silver Nanoparticles and Their Characterization

Preparation of Silver Nanoparticles and Their Characterization Preparation of Silver Nanoparticles and Their Characterization Abstract The preparation of stable, uniform silver nanoparticles by reduction of silver ions by ethanol is reported in the present paper.

More information

Detonation Nanodiamond Suspensions

Detonation Nanodiamond Suspensions PRODUCT SHEET Rev. 10/17, v3.0 Detonation Nanodiamond Suspensions Colloidal suspensions of detonation nanodiamond (DND) in both and a variety of organic solvents have a wide range of uses, including: (1)

More information

Supporting Information

Supporting Information Supporting Information Highly Sensitive, Reproducible, and Stable SERS Sensors Based on Well-Controlled Silver Nanoparticles Decorated Silicon Nanowire Building Blocks Xue Mei Han, Hui Wang, Xue Mei Ou,

More information

Electronic supplementary information for:

Electronic supplementary information for: Electronic supplementary information for: Charge-transfer-induced suppression of galvanic replacement and synthesis of (Au@Ag)@Au double shell nanoparticles for highly uniform, robust and sensitive bioprobes

More information

ALGINATE MICRO-BIOREACTORS DELIVERING PROTEINS: AN EXAMPLE DRUG DELIVERY SYSTEM

ALGINATE MICRO-BIOREACTORS DELIVERING PROTEINS: AN EXAMPLE DRUG DELIVERY SYSTEM ALGINATE MICRO-BIOREACTORS DELIVERING PROTEINS: AN EXAMPLE DRUG DELIVERY SYSTEM Chen, Guisang Daphne and Wang, Chi-Hwa Chemical & Biomolecular Engineering National University of Singapore 1 Lower Kent

More information

Journal of Colloid and Interface Science

Journal of Colloid and Interface Science Journal of Colloid and Interface Science 35 (2011) 78 82 Contents lists available at ScienceDirect Journal of Colloid and Interface Science www.elsevier.com/locate/jcis Diffusion through colloidosome shells

More information

International Journal of Pure and Applied Sciences and Technology

International Journal of Pure and Applied Sciences and Technology Int. J. Pure Appl. Sci. Technol., 9(1) (2012), pp. 1-8 International Journal of Pure and Applied Sciences and Technology ISSN 2229-6107 Available online at www.ijopaasat.in Research Paper Preparation,

More information

Seeing the Nano-scale: Nanoparticle Tracking Analysis HVM MNT Expo 2006 Oxford. Jeremy Warren CEO, NanoSight Ltd

Seeing the Nano-scale: Nanoparticle Tracking Analysis HVM MNT Expo 2006 Oxford. Jeremy Warren CEO, NanoSight Ltd Seeing the Nano-scale: Nanoparticle Tracking Analysis HVM MNT Expo Oxford Jeremy Warren CEO, NanoSight Ltd 1 NanoSight Particle Characterisation at Nano-scale: Electron Microscopy PCS (Photon Correlation

More information

Pickup velocity of nanoparticles

Pickup velocity of nanoparticles Engineering Conferences International ECI Digital Archives Fluidization XV Proceedings 5-23-2016 Pickup velocity of nanoparticles Jia Wei Chew Singapore Membrane Technology Center, Nanyang Environment

More information

Methods for charge and size characterization colloidal systems

Methods for charge and size characterization colloidal systems Methods for charge and size characterization colloidal systems Content General Basics Stabino Measurement basics Applications NANO-flex Measurement basics Applications Nanoparticles Bulkphase of gold gold

More information

Part I: Short answer (25 points)

Part I: Short answer (25 points) Part I: Short answer (25 points) Part II: / 75 1. Recently, researchers published a five-year study involving 2,687 subjects with a disorder called sleep apnea. The subjects were between the ages of 45

More information

Solutions. Experiment 11. Various Types of Solutions. Solution: A homogenous mixture consisting of ions or molecules

Solutions. Experiment 11. Various Types of Solutions. Solution: A homogenous mixture consisting of ions or molecules Solutions Solution: A homogenous mixture consisting of ions or molecules -Assignment: Ch 15 Questions & Problems : 5, (15b,d), (17a, c), 19, 21, 23, 27, (33b,c), 39, (43c,d),45b, 47, (49b,d), (55a,b),

More information

Supporting Information. For. Preparation and Characterization of Highly Planar Flexible Silver

Supporting Information. For. Preparation and Characterization of Highly Planar Flexible Silver Supporting Information For Preparation and Characterization of Highly Planar Flexible Silver Crystal Belts Dharmesh Varade and Kazutoshi Haraguchi* [*] Dr. K. Haraguchi (Corresponding-Author), Dr. D. Varade

More information

Determination of redox reaction rates and orders by in-situ liquid cell. electron microscopy of Pd and Au solution growth

Determination of redox reaction rates and orders by in-situ liquid cell. electron microscopy of Pd and Au solution growth BNL-107596-2014-JA Determination of redox reaction rates and orders by in-situ liquid cell electron microscopy of Pd and Au solution growth Eli A. Sutter * and Peter W. Sutter Center for Functional Nanomaterials,

More information

Multiple Choice 2 POINTS EACH Select the choice that best answers the question. Mark it clearly on your answer sheet.

Multiple Choice 2 POINTS EACH Select the choice that best answers the question. Mark it clearly on your answer sheet. Chemistry 45.5 100 Points Take Home Exam 1 2009-10 Name: Student ID: Form A Multiple Choice 2 POINTS EACH Select the choice that best answers the question. Mark it clearly on your answer sheet. 1. Likes

More information

Technology, Techniques and Applications. Ric Allott Business Development Manager

Technology, Techniques and Applications. Ric Allott Business Development Manager Technology, Techniques and Applications Ric Allott Business Development Manager 1 Central Laser Facility ASTRA GEMINI VULCAN ARTEMIS ULTRA OCTOPUS High power, ultrashort pulse dual beams of 15 J, 30 fs

More information

Supplementary Information for. Blood-Clotting Inspired Reversible Polymer-Colloid. Composite Assembly in Flow

Supplementary Information for. Blood-Clotting Inspired Reversible Polymer-Colloid. Composite Assembly in Flow Supplementary Information for Blood-Clotting Inspired Reversible Polymer-Colloid Composite Assembly in Flow Hsieh Chen 1, Mohammad A. Fallah 2,3, Volker Huck 4, Jennifer I. Angerer 2,3, Armin J. Reininger

More information

Efficient charge storage in photoexcited TiO 2 nanorod-noble metal nanoparticle composite systems

Efficient charge storage in photoexcited TiO 2 nanorod-noble metal nanoparticle composite systems Electronic Supplementary Information Efficient charge storage in photoexcited TiO 2 nanorod-noble metal nanoparticle composite systems P. D. Cozzoli, M. L. Curri, and A. Agostiano 1. Synthesis of TiO 2

More information

Solvent does the dissolving (acetone) Solute the substance being dissolved (Styrofoam ) Soluble able to be dissolved

Solvent does the dissolving (acetone) Solute the substance being dissolved (Styrofoam ) Soluble able to be dissolved Solvent does the dissolving (acetone) Solute the substance being dissolved (Styrofoam ) Soluble able to be dissolved Like dissolves Like Ionic & polar compounds dissolve each other. Nonpolar dissolves

More information

Magnetic nanoparticles-induced anisotropic shrinkage of polymer emulsion droplets. Table of contents

Magnetic nanoparticles-induced anisotropic shrinkage of polymer emulsion droplets. Table of contents Electronic Supplemental Information Magnetic nanoparticles-induced anisotropic shrinkage of polymer emulsion droplets Bing Liu, * a Julius W.J. de Folter b and Helmuth Möhwald a a Department of Interfaces,

More information

Electronic Supporting Information. Microcapsule Buckling Triggered by Compression- Induced Interfacial Chase Change

Electronic Supporting Information. Microcapsule Buckling Triggered by Compression- Induced Interfacial Chase Change Electronic Supporting Information Microcapsule Buckling Triggered by Compression- Induced Interfacial Chase Change Andrew R Salmon, 1 Richard M Parker, 1 Alexander S Groombridge, 1 Armando Maestro, 1 Roger

More information

COLLIGATIVE PROPERTIES

COLLIGATIVE PROPERTIES COLLIGATIVE PROPERTIES Depend on the number of solute particles in solution but not on the identity of the solute Vapor pressure lowering Boiling point elevation Freezing point depression Osmotic pressure

More information

Nanocontainers with controlled permeability for feedback active coatings

Nanocontainers with controlled permeability for feedback active coatings Nanocontainers with controlled permeability for feedback active coatings Contents: 1. Self-healing coatings based on nanocontainers for corrosion protection: - with ph-triggered release, - triggered by

More information

Jacek Balcerzak, Maria Mucha

Jacek Balcerzak, Maria Mucha ANALYSIS OF MODEL DRUG RELEASE KINETICS FROM COMPLEX MATRICES OF POLYLACTIDE-CHITOSAN Jacek Balcerzak, Maria Mucha Technical University of Lodz, Faculty of Process and Environmental Engineering Wolczanska

More information

- Supporting Information - Controlled Assembly of Eccentrically Encapsulated Gold Nanoparticles

- Supporting Information - Controlled Assembly of Eccentrically Encapsulated Gold Nanoparticles - Supporting Information - S1 Controlled Assembly of Eccentrically Encapsulated Gold Nanoparticles Tao Chen, Miaoxin Yang, Xinjiao Wang, Li Huey Tan, Hongyu Chen* Division of Chemistry and Biological Chemistry,

More information

1. Which substance will conduct the current in the solid state? 1. Diamond 2.Graphite 3.Iodine 4.Sodium chloride.

1. Which substance will conduct the current in the solid state? 1. Diamond 2.Graphite 3.Iodine 4.Sodium chloride. CHAPTER :SOLIDS 1. Which substance will conduct the current in the solid state? 1. Diamond 2.Graphite 3.Iodine 4.Sodium chloride. 2. Molten sodium chloride conducts electricity due to the presence of ions:

More information

Chapter 3.1 Chemistry of Life

Chapter 3.1 Chemistry of Life Life Science Chapter 3: Cell Processes 1. Chemistry of Life 2. Moving Cellular Materials 3. Energy for Life http://www.connecticutvalleybiological.com/cell processes vhs p 14026.html Chapter 3.1 Chemistry

More information

Kelly Robinson 1 and Ravi Sharma 2

Kelly Robinson 1 and Ravi Sharma 2 Electrophoretic Mobility Estimated from the Transient Current in a Parallel Plate Cell Kelly Robinson 1 and Ravi Sharma 1 Electrostatic Answers LLC Rochester, NY 14450, USA email: Kelly.Robinson@ElectrostaticAnswers.com

More information

Deposition of Titania Nanoparticles on Spherical Silica

Deposition of Titania Nanoparticles on Spherical Silica Journal of Sol-Gel Science and Technology 26, 489 493, 2003 c 2003 Kluwer Academic Publishers. Manufactured in The Netherlands. Deposition of Titania Nanoparticles on Spherical Silica DONG HWAN RYU, SEONG

More information

Fabrication of SiO 2, Al 2 O 3, and TiO 2 Microcapsules with Hollow Core and Mesoporous Shell Structure

Fabrication of SiO 2, Al 2 O 3, and TiO 2 Microcapsules with Hollow Core and Mesoporous Shell Structure Fabrication of SiO 2, Al 2 O 3, and TiO 2 Microcapsules with Hollow Core and Mesoporous Shell Structure Xiao-Feng Guo, Yong-Suk Kim, and Geon-Joong Kim Department of Chemical Engineering, Inha UniVersity,

More information