L Mott SALT WATER AQUACULTURE. Model AQ-4/AQ-5 Code / test kit instruction manual

Transcription

1 L Mott SALT WATER AQUACULTURE test kit instruction manual Model AQ-4/AQ-5 Code /

2 This booklet provides step-by-step detailed instructions for the Model AQ-4 (Code ) and AQ-5 (Code ) test kits. It is important to review these instructions thoroughly before attempting to perform the tests by the short-form instructions contained in the case lid. TABLE OF CONTENTS PAGE TESTING HINTS...3 ANALYTICAL TECHNIQUES...4 SAFETY INFORMATION...5 TEST METHODS...6 TEST PROCEDURES...8 Introduction...8 Alkalinity...9 Ammonia Nitrogen...11 Carbon Dioxide...16 Dissolved Oxygen Nitrate Nitrogen Nitrite Nitrogen...24 ph...26 Salinity...28 DIAGRAMS AQ AQ

3 TESTING HINTS REAGENT CARE 3

4 ANALYTICAL TECHNIQUES X 4

5 SAFETY INFORMATION 5

6 TEST METHODS This tesst kit uses two basic analtical procedures common to field test kits. A brief explanation of each follows: COLORIMETRIC: OCTA-SLIDE VIEWER In a visual colorimetric test, a sample is treated with reagent(s) to produce a color reaction, generally in proportion to the amount of test factor present. The sample color is then compared against color standards representing known concentrations of the factor being tested over a specific range. The all-plastic Octa-Slide Viewer allows for a unique one-on-one color matching system. USEOFTHEOCTA-SLIDEVIEWER DILUTIONS The calibrated test tubes (0106) included in this kit may be used to perform dilutions for the Ammonia Nitrogen and Nitrite Nitrogen tests. Distilled or deionized water is needed to perform dilutions. The following table provides a quick reference guide for dilutions of various proportions. Once the dilution is prepared, use this diluted sample to perform the test, and multiply the result by the dilution factor to obtain the actual concentration. Sample Size Distilled Water to Bring to 10 ml Dilution Factor 5.0 ml 5.0 ml ml 7.5 ml 4 6

7 TITRIMETRIC: DIRECT READING TITRATOR The Titrator consists of a plastic barrel, a plastic plunger, and a plastic adapter tip. The adapter tip reduces the size of the drops that are dispensed, increasing the precision of the test results. DO NOT the plunger or adapter tip from the Titrator. In a titrimetric method, titrating solution (or titrant) is added to a treated sample until a color change occurs. The volume of titrant required to reach this endpoint is proportional to the concentration of the factor being tested. Direct Reading Titrators provide results directly in the appropriate concentration for the test - no counting of drops, no calculations. USE OF THE DIRECT READING TITRATOR 7

8 TEST PROCEDURES INTRODUCTION Proper control of water quality is an essential part of a successful aquaculture operation. Immediate test results provided by on-site water analysis equipment can confirm a healthy environment, or give early warning signals for required treatment. Develop a routine testing schedule. Keep records! Historical data is extremely important if treatments are required. Note environmental conditions, fish activity, feeding habits, etc. Observe fish to note any peculiar behavior or feeding rates, as this may be a sign of stress. Stable characteristics, such as alkalinity and hardness, do not have to be tested as frequently as ones that fluctuate, such as ammonia nitrogen, nitrite nitrogen, ph, dissolved oxygen and temperature. Keep in mind that these factors fluctuate throughout the day and are in some cases interdependent. Be alert to sudden changes in one factor, as it may be a clue to perform further analysis. 8

9 ALKALINITY REAGENTS AND APPARATUS DESCRIPTION CODE *BCG/MR Indicator *2311-EG-E *Alkalinity Titration Reagent B *4493DR-H Titration Tube, 5, 10, 12.9, 15, 20 & 25 ml, glass w/cap 0608 Direct Reading Titrator 0382 *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. The Titrator is calibrated in terms of total alkalinity expressed as parts per million (ppm) Calcium Carbonate (CaCO 3 ). Each minor division on the Titrator scale equals 4 ppm CaCO 3. 9

10 ALKALINITY TEST PROCEDURE 10

11 AMMONIA NITROGEN REAGENTS AND APPARATUS DESCRIPTION CODE *Salicylate Ammonia #1 *3978LWT-G *Salicylate Ammonia #2 *3979WT-G Salicylate Ammonia #3 3982WT-G Test Tube, 2.5, 5.0 & 10.0 ml, plastic, w/cap 0106 Octa-Slide Viewer 1100 Octa-Slide, Ammonia Nitrogen, ppm 3441 *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. AMMONIA NITROGEN TEST PROCEDURE 11

12 AMMONIA NITROGEN TEST PROCEDURE 12

13 AMMONIA IN AQUARIUMS Ammonia in water occurs in two forms: toxic unionized ammonia (NH 3 ) and the relatively non-toxic ionized form, ammonium ion (NH4+). This test method measures both forms as ammonia-nitrogen (NH4 + -N) to give the total ammonia-nitrogen concentration in water. The actual proportion of each compound depends on temperature, salinity, and ph. A greater concentration of unionized ammonia is present when the ph value and salinity increase. 1. Consult the table below to find the percentage that corresponds to the temperature, ph and salinity of the sample. 2. To express the test result as ppm Unionized Ammonia Nitrogen (NH 3 -N), multiply the total ammonia-nitrogen test result by the percentage from the table. 3. To express the test result as ppm Ammonia Nitrogen (NH4 + -N), subtract the unionized ammonia-nitrogen determined in step 2 from the total-ammonia-nitrogen. 10 C 15 C 20 C 25 C ph FW 1 SW 2 FW SW FW SW FW SW Freshwater data from Trussel (1972). 2 Seawater values from Bower and Bidwell (1978). Salinity for Seawater values = 34% at an ionic strength of m. Conversions: To express results as Unionized Ammonia (NH 3 ): ppm Ammonia Nitrogen (NH 3 -N) x 1.2 = Ammonia (NH 3 ) To express results as Iodized Ammonium(NH 4 + ): ppm Ammonia Nitrogen (NH 3 -N) x 1.3= Ammonium (NH 4+ ) 13

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16 CARBON DIOXIDE REAGENTS AND APPARATUS DESCRIPTION CODE *Phenolphthalein Indicator, 1% *2246-E *Carbon Dioxide Reagent B *4253DR-H Direct Reading Titrator, Titration Tube, 5, 10, 12.9, 15, 20 & 25 ml, glass, w/cap 0608 *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. The Titrator is calibrated in terms of carbon dioxide expressed as ppm free CO 2. Each minor division on the Titrator scale equals 1.0 ppm CO 2. 16

17 CARBON DIOXIDE TEST PROCEDURE 17

18 DISSOLVED OXYGEN REAGENTS AND APPARATUS DESCRIPTION CODE *Manganous Sulfate Solution 4797-WT-G *Alkaline Potassium Iodide Azide Reagent *7166-G *Sulfuric Acid, 1:1 *6141WT-G *Sodium Thiosulfate Solution (0.025N). *4169-H Starch Indicator 4170PS-G Direct Reading Titrator, Titration Tube, 5, 10, 12.9, 15, 20 & 25 ml, glass, w/cap 0608 Pipet, plain, plastic, w/cap 0392 Water Sampling Bottle, 60 ml, glass 0688-DO *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. The Titrator is calibrated in terms of dissolved oxygen expressed as ppm dissolved oxygen. Each minor division on the Titrator scale equals 0.2 ppm dissolved oxygen. Included only in Model AQ-4, Code

19 SAMPLING AND PRESERVATION 19

20 DISSOLVED OXYGEN TEST PROCEDURE 20

21 DISSOLVED OXYGEN TEST PROCEDURE 21

22 NITRATE NITROGEN REAGENTS AND APPARATUS DESCRIPTION CODE *Mixed Acid Reagent *V-6278-J *Nitrate Reducing Reagent *V-6279-J Spoon, 0.1g, plastic 0699 Test Tube, 2.5, 5.0 & 10.0 ml, plastic, w/cap 0106 Octa-Slide Viewer 1100 Octa-Slide, Nitrate Nitrogen, ppm 3413 Dispenser Cap 0692 *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. NITRATE NITROGEN TEST PROCEDURE 22

23 NITRATE NITROGEN TEST PROCEDURE 23

24 NITRITE NITROGEN REAGENTS AND APPARATUS DESCRIPTION CODE *Mixed Acid Reagent *V-6278-H *Color Developing Reagent *V-6281-D Spoon, 0.1g, plastic 0699 Test Tube, 2.5, 5, & 10, plastic, w/cap 0106 Octa-Slide Viewer 1100 Octa-Slide, Nitrite Nitrogen, ppm 3437 *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. 24

25 NITRITE NITROGEN TEST PROCEDURE 25

26 ph REAGENTS AND APPARATUS DESCRIPTION CODE *Wide Range Indicator *2218-G Test Tube, plastic, 2.5, 5.0 & 10 ml, w/cap 0106 Octa-Slide Viewer 1100 Wide Range ph Octa-Slide Bar, *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. Included only in Model AQ-4, Code

27 ph TEST PROCEDURE 27

28 SALINITY REAGENTS AND APPARATUS DESCRIPTION CODE *Salinity Indicator Reagent A *7460-E *Salinity Titration Reagent B *7461-H Demineralizer Bottle, Model PWB Titration Tube, 5, 10, 12.9, 15, 20 & 25 ml, glass w/cap 0608 Direct Reading Titrator, Direct Reading Titrator, *WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for these reagents. For your safety, read label and accompanying MSDS before using. DEMINERALIZER BOTTLE The bottle is partially filled with an ion exchange resin containing an indicator to show when the exchange resin has been exhausted. The indicator will change from dark green to yellow amber at which point the resin should be replaced. INSTRUCTIONS FOR USE 1. Remove cap and fill bottle with water to be demineralized. 2. Recap, make sure spout is closed, and shake vigorously for 30 seconds. 3. Open spout, invert bottle, and gently squeeze to dispense the demineralized water. DEMINERALIZER CARE A. Do not heat water over 100 F. B. Keep the resin covered with water at all times. C. Leave the bottle fairly full when storing. 28

29 SALINITY TEST PROCEDURE 29

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