QUIZ 4 REVIEW QUIZ (DEC 7)

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1 PHYSICAL CHEMISTRY LAB CHEM343 LECTURE 6 (11/30/12) Class Schedule/Grading Flash Presentation Nano diffraction SCHEDULE Final Exam Schedule Dec 13 (Thr) From 1 PM (2hours) at 130 SES; ~60 % is multiple choice questions Final PowerPoint Presentation The last day (W/R) Dec 5(W) or 6(R) at the PchemLab (8AM sec Prepare in advance & Make sure you have the data! Quiz 4 (Dec 7): All the 6 experiments + lecture Quiz 3 Today! (Flash + Nano diffraction) Please turn in your LAB Notebook at the time of Final Presentation for grading Please claim any corrections for Rot 4 before the final presentation (Talk to the TA who graded the report) For Rot 5, claim any corrections before the Final Exam (No corrections will be made after that) 1

2 QUIZ 4 REVIEW QUIZ (DEC 7) Mass specs & Flash plus all the experiments Format (a) Multiple Choice (b) Calculations (c) Both Number of Questions (a) About 10 (b) About 20 (c) About 30 We do not give you the answer because we plan to pick iksome questions for the Final lexam GRADING Lab Works: 100 points Experiments: 900 points (for each experiment: 10 for pre-lab discussion, 20 for pre-lab quiz, 20 for performance and 100 for report) Notebook: 150 (turn-in at the end of lab) Lecture: Up to 20 points for Attendance; 80 points for quiz from lectures Quiz: 80 points ex. Q1: 20 Q2: 12 Q3: 12 Q4: 36 (18x2) Total 80 Final: 200 points Total: 1450 points 2

3 FLASH PHOTOLYSIS Remember the figure! FLASH PHOTOLYSIS Q1 Q2 Q3 Q4 Q5 Q6. What is the difference between internal conversion & fluoresecence? Internal conversion accompanies no emission. 3

4 FLASH APPARATUS Q1 Q3 Q2 MANY SPECIES. WHAT IS THE ROLE? Q1. What is detected? (a) (b) (c) (d) Q2. What is formed by a reaction of T 1 and ROH? Choose one. Q3. What is the reaction responsible for the decay of T 1? (a) (b) (c) 4

5 REACTION Q4. Which reaction does the flash cause? (a) (b) Q5. Formation of a protonated ketyl radical from benzophenone requires the presence of (a) alcohol l group (b) ketone group (c) acid group REACTION SCHEME Flash Q1 Intersystem crossing 5

6 EXPERIMENT PROTOCOL QUESTIONS Why do we bubble N 2 gas? What is purged out? Why did we have to work in the dark for this lab? What kind of value do you read from the computer display? How did we convert it into absorbance? POWERPOINT PRESENTATION 13 min Presentation (15-20 slides) 3-5 min for Questions Graded based on Science (70 points) & Presentation (30 points) Do NOT copy other student s slides! 6

7 POWERPOINT PRESENTATION Items to be included? Title (Exp name & Your names) Intro + Objective of the exp (Experimental) Spectra & Data analysis Reasoning (think what is requested) Show examples (choose effective ex.) Answers to the Questions in the Report If questions are not clear, ask your TA Conclusion Style: Two students give one presentation Each student explains one slide in turn GRADING FOR THE PRESENTATION? Science 70 points Presentation 30 points Finished on-time ( /5) Font size, Good graphs & tables etc (# of significance -2, axis/units in graph -2, font too small -2) ( /5) Students take turns? ( /5) Effective slides? ( /5) Clear explanation & presentation style? ( /5) Answering questions from the Audience? ( /5) 7

8 SCORE GUIDELINES FOR 5 EXPERIMENTS Please ask questions to TA who is responsible for your experiment for the further details NMR Presentation Score Sheet Introduction [ /15] The objective of the experiment presented ( ) Background of the experiment well introduced? ( ) (Mention: Spin magnetic moment, FT NMR, 13 C/ 1 H NMR, Molecular diffusion) Spectra, Data Analysis, Signal Assignment [ /28] Analysis of 13 C NMR Analysis of 1 H NMR Analysis of DEPT 45, 90, 135 Analysis of 2D HETCOR Analysis of 2D COSY How you analyzed & how you used the data for assignment Showing data Molecular Diffusion by Field Gradient [ /12] Data analysis Interpretation Questions in the Report (answer at least 2) [ /10] What is the unknown sample? How 2D NMR changed the way of analysis? Was molecular diffusion useful for assignment? Conclusion [ /5] + Presentation 30 points 8

9 FIRST AS USUAL Analyze & organize the data Data (Scan data/obtain pdf, jpg, ps files) Scanners are available at SEL computer lab Graphs (Excel etc) You can embed Excel graphs into you presentation Table Insert Table Copy Excel Table & Edit Paste Special Write an Outline (Do not start from PP) Think about how to fit your data within 13 mins USE GRAPH & TABLES EFFECTIVELY 9

10 CONS & PROS OF POWERPOINT Pros More information in a slide Easy to modify your show Animation & background Cons A computer always freezes at your presentation Come early and check your slides using the computer Keep USB backup (pdf, pp) and prepare handouts Turn off screen saver Yes, useful!! Disaster! BACKGROUND FOR DUMMYS Dark background is often effective, but your audience will soon feel sleepy if you use only dark background Changing a background wakes your audience up Too colorful/decorative background may be distractive 10

11 BACKGROUND FOR DUMMYS Dark background is often effective, but your audience will soon feel sleepy if you use only dark background Changing a background sometimes wakes your audience up Too colorful/decorative background may be distractive BACKGROUND FOR DUMMYS Dark background is often effective, but your audience will soon feel sleepy if you use only dark background Changing a background sometimes wakes your audience up Too colorful/decorative background may be distractive 11

12 FIGURES How to prepare figures? Autoshape Line Arrow Picture/Graphs FIGURES 12

13 FIGURES You can draw reasonable figures with Shapes, Line, Arrows + Light In EFFECTIVE COMBINATION (PICTURE+TEXT) Incubation-time dependence of self-assembly of Alzheimer s amyloid peptide p 0 h (Monomer) 30 h (Intermediate) 50 h 72 h (Amyloid fibrils) 100 nm 13

14 EFFECTIVE COMBINATION (+ARROWS) Incubation-time dependence of self-assembly of Alzheimer s amyloid peptide 0 h (Monomer) 30 h (Intermediate) 50 h 72 h (Amyloid fibrils) 100 nm Amyloid intermediate: Neuro-toxin Diameter nm Amyloid fibril: Major component of Alzheimer s plaque Diameter 10 nm EFFECTIVE COMBINATION (+ ANIMATION) Incubation-time dependence of self-assembly of Alzheimer s amyloid peptide 0 h (Monomer) 30 h (Intermediate) 50 h 72 h (Amyloid fibrils) 100 nm Amyloid intermediate: Neuro-toxin Diameter nm Amyloid fibril: Major component of Alzheimer s plaque Diameter 10 nm 14

15 PRESENTATION Some Suggestions Remove unnecessary words & shorten sentences Summarize at the end Do not use Yellow Unless you use dark background Practice, practice Answer questions! (You need to take turns) NANO DIFFRACTION LAB SET-UP Q. What kind of laser do we use? (wave length etc) Q2. What is this? What is the function? 15

16 FRAUNHOFER DIFFRACTION: SINGLE & DOUBLE SLIT a D d ~ /d BRAGG S LAW OF DIFFRACTION If light reflects off different atoms in a crystal lattice, there exist certain intervals where the light will constructively interfere. These intervals are specified by the Bragg equation. Constructive interference is directly observable (they show up as bright spots on a screen) By looking at the pattern, it is possible to determine the structure of a crystal that caused it to form. For specific angles that satisfy the Bragg equation, a different d value is obtained. This d value is called the lattice spacing. For any periodic array of crystals, there are multiple d-spacings in which the light will diffract. For 3-D constructive interference: 2 d sin = n For a 2-D lattice: d sin = n Principal maxima of a diffraction grating sin m, m 1, 2, 3,... d d 16

17 MILLER PLANES Atoms form periodically arranged planes Parallel planes are equivalent Any set of planes is characterized by: (1) their orientation in the crystal (hkl) Miller indices (2) their d-spacing d (d hkl ) distance between the planes h, k, l correspond to the number of segments in which the a, b, c axes, respectively, are cut by the set of planes for cubic crystal (a h =a k =a l, 90 ): 1/d hkl2 = (h 2 + k 2 + l 2 )/a 2 1/d 2 =(h +k hk2 2 2 )/a 2 (for 2D) for hexagonal*: 1/d hk2 = 4/3 (h 2 + k 2 +hk)/a 2 2-D Examples (for rectangular) Q1. What is the index for the lattice below? (a)(1, 3) (b) (3, 1) (c) (1, 1) (d) (3, 3) Q2. Assume that the lattice is a cubic with a h = a k = a. How much is d? (a) a (b) a( ) 1/2 (c) a/(1 1/2 +3 1/2 ) 2 (d) a/( ) 1/2 d hk = a/(h 2 + k 2 ) 1/2 17

18 DIFFRACTION NOMENCLATURE: PATTERNS Single crystal vs. powder diffraction Powder diffraction (left) scatters incident radiation off of multiple 2-D domains. This causes a ring-like pattern. Diffraction from a single domain (right) shows dot patterns as only one domain orientation is studied (as single domain can only be oriented one way). More precise and specialized data can be obtained from this than a powder diffraction pattern. Q2. What is this? Q. What kind of pattern is expected from Multi domain? Beam size Q3. What kind of pattern from single domain? The experiment (2) The sample is placed between the laser with lens and a clipboard. Place a lens about six inches in front of the sample (between the sample and the laser); make sure the laser goes through the center of the lens. The laser beam focuses on the sample to allow diffraction off a single domain that forms a spot diffraction pattern. Consider about relationship between beam size, domain size and particles sizes lens laser 18

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