Biotage 2012 Webinar series

Transcription

1 Biotage 2012 Webinar series Title: Isolera Spektra - A Revolution in Intelligent Flash Chromatography Date: 13 th June 2012 Summary: Automated flash has until today been based upon simple evolutionary improvements from traditional glass columns. The aim of this presentation is to show chemists how to fully access the performance of their flash cartridges, significantly increasing reproducibility, reducing laboratory running costs and time for purification.

2 Sunil Rana - Bio Academic Double Honours B.Sc. Chemistry and Biochemistry Ph.D. (organic, peptide and polymer chemistry) (Professor Mark Bradley) Industrial Industrial Post-Doc Argonaut Technologies, USA Argonaut Technologies, USA Discovery Chemistry Group, Biotage, UK Senior Scientist / Project Manager, Biotage GB Ltd 2009-present Product Manager, Chemistry Applications and Resins RSC and ACS member (10+ years) author/co-author of 10+ publications/ patents, established speaker and trainer, with interests in flash chromatography, organic and peptide chemistry, resins for use in pharmaceutical research, work-up techniques and metal scavenging slide 2

3 Intelligent Advances in Flash Chromatography Sunil Rana, MRSC, Ph.D June 2012

4 Background» Historical Bottlenecks in Drug Development Chemical Diversity: combinatorial methods, HTC, libraries Work-up: parallel work up tools, common scaffolds Purification: Flash purification slide 4

5 Flash Chromatography» Development has been two fold: Column Technology Instrumentation slide 5

6 A Brief History» s Earliest crude examples» 1901 Tsvet first true chromatography, plant pigments» More developments» 1952 Partition theory, (Nobel Prize) Martin and Synge» 1960 s Birth of HPLC» 1978 Clark Still / silica particles in flash» 1994 Biotage introduces pre-packed flash cartridges W. Clark Still, Michael Kahn, Abhijit Mitra J. Org. Chem., 1978, 43 (14), pp General information accessed 24 May 2012: slide 6

7 Why Pre-packed Cartridges? Many users look for improvements in: Speed: Need to run a faster separation Safety: Handling loose silica, pressurizing glass columns Reliability: Not reproducible, variations, relies on skill for excellent results Green Credentials: Reduction of solvent and waste, reduction of evaporation time of solvents Real-time: Manual analysis post column Flexibility: Fixed glassware size, not always efficient Process: Not easy to control Photograph information accessed 24 May 2012: slide 7

8 The Chromatographers Triangle Increasing yield: reduces purity and also can reduce throughput Increasing throughput: increases flow rate / pressure, reduces efficiency, reduces purity Increasing purity: reduces yield, and lowers throughput 3 conflicting dimensions slide 8

9 Column Technology» Increasing demand for higher-performance flash cartridges with spherical silica Sharper peaks Better loading capacity Faster throughput Purer compounds Reduced fraction volumes Reduced solvent use But these needs Contravene the basic rules of the triangle! Can we escape these rules? slide 9

10 Paradigm Shift: HP-Sphere Silica» Recent Developments 25 micron spherical particles: higher resolution Does not generate fines: lower backpressure, higher throughput 40% higher surface area: higher loading Std flash silica NOTE: HP-Sphere, the unique new silica is ONLY available in Biotage SNAP-Ultra flash cartridges slide 10

11 Biotage SNAP Ultra vs. Irregular Silica» Increased surface area Increase loading capacity Use a smaller cartridge Less CV, less volume to evaporate afterwards Biotage SNAP Ultra 25g, 25 ml/min 4% load 0.5 bar (7.25 psi)» Small, 25 µm particle size increases resolving power Closer to baseline separation (for the same load) Irregular silica cartridge, 25um, 25g, 25 ml/min 2% load 1 bar (14.5 psi)» Lower operating pressure compared to std silica slide 11

12 Pressure (bar) Pressure vs. Flow Rate SNAP Ultra ~1/2 operating pressure of std spherical silica particles 6 Pressure vs. Flow 5 Conditions Heptane/EtOAc 93: ml/min 40 ml/min 60 ml/min 80 ml/min 100 ml/min Flow Rate BIOTAGE SNAP ULTRA LOWEST OPERATING PRESSURE VS FLOW RATE slide 12

13 Low Backpressure: Better Plates / Higher Flow» Either maintain resolution» And / Or improve purification throughput» And / Or increase productivity» While maintaining low operating pressure Reduce purification costs Standard run Biotage SNAP Ultra 25g, 25 ml/min Maximum productivity Biotage SNAP Ultra 25g, 150 ml/min Runtime: min Runtime: 2.86 min FLOW RATE α PLATES AND EFFICIENCY HIGH EFFICIENCY CARTRDIGE slide 13

14 Instrumentation: Automated Flash Highlights 1990 s early instruments spawned an industry of automated flash Key Features» Pump control replacing bellows, providing gradients» Fraction Collection reducing labour» Post column UV analysis reducing fraction consolidation time The intelligent ones re-write the rule book...» Extract more value from pre-packed flash cartridges.» Save time, solvent and money without re-optimizing chemistry slide 14

15 Flash Chromatography Work Flow slide 15

16 Intelligent Features Unlock Potential» Step gradient Save 30-50% time, solvent and reduce costs» TLC STEP gradient From TLC plate straight to optimized flash method Paradigm shift, target product directly, control the run, don t just respond to it waiting for fractions» Advanced collect all with baseline correction Improve yields Reduce evaporation time Collect product not solvent» PDA review Is that single peak really pure? Adding new dimensions to flash slide 16

17 Step Gradients Save Time And Solvent» Normal Linear TLC 30% B Wasted volume 7% B for 1 CV 7-60% B in 10 CV 60% B for 2 CV» Isolera Spektra Step gradient 16% B 2CV 27% B 2 CV 41% B 3 CV» Run volume estimation Linear 195 ml (13 CV) Step 105 ml (7 CV)» STEP SOLVENT SAVINGS 7 CV (54% of run volume) slide 17

18 Optimized Scale-Up STEP 1: Run 50g cartridge 13 CV linear gradient = 858 ml 50g SNAP, Linear gradient, 13 CV STEP 2: Scale to 1500g cartridge 13 CV linear gradient = STEP 2: Target Peak Or And STEP 3: Isolera re-calculates STEP gradient CV step gradient = 9.99 L 21% B 1 33% B Savings = L Same sample Complete separation 50% less solvent 1500g SNAP, step gradient, peak 2 target, 5 CV slide 18

19 From TLC Straight to Optimized Step The video TLC to step4 (45 seconds) should be played here slide 19

20 Baseline Correction The video baseline correction (28 seconds) should be played here slide 20

21 Collect Product, Not Solvent» Spinach extract» λ-all and Baseline correction features Less fractions Higher concentration fractions Less solvent to evaporate No baseline correction λ-all 430 nm 459 nm Baseline correction slide 21

22 Baseline Drift - Eliminated» Acetone, Toluene are effective solvents for organic compounds» Not used in flash due to absorbance in UV regions of interest» Baseline correction now allows use of UV absorbing solvents slide 22

23 PDA Scan Review: Is That Single Peak Really Pure? The video lambda pure peak2 (23 seconds) should be played here slide 23

24 PDA Scan Review: Is That Single Peak Really Pure? The video lambdaimpurepeak (37 seconds) should be played here slide 24

25 Adding New Dimensions To Flash The video 2d to 3d view (44 seconds) should be played here slide 25

26 Summary Recent Developments allow purifications to be carried out faster, in smaller more efficient cartridges (SNAP Ultra), using even less solvent. Cutting-edge flash instruments (Isolera Spektra) allows chemists to realise even more performance from flash cartridges; resulting in more efficient methods, targeted products, saving more time, solvent and money. slide 26

27 For Further Information Biotage 1-Point Support Contacts North America Phone: Outside US: US-1-pointsupport@biotage.com Europe Phone: Fax: EU-1-pointsupport@biotage.com Japan Phone: Fax: JP-1-pointsupport@biotage.com Isolera Spektra China Phone: Fax: CN-1-pointsupport@biotage.com For more information on this webinar or to receive information about cutting edge concepts in flash chromatography...please contact your local Biotage representative slide