K Basavaiah * & U Chandrashekar
|
|
- Blaise Walters
- 6 years ago
- Views:
Transcription
1 Indian Journal of Chemical Technology Vol. 12, July 2005, pp Sensitive micro analysis of frusemide (furosemide) in bulk drug and formulations by visible spectrophotometry and high performance liquid chromatography (HPLC) K Basavaiah * & U Chandrashekar Department of Chemistry, University of Mysore, Manasagangotri, Mysore , India Received 25 August 2004; revised received 10 January 2005; accepted 3 March 2005 Two rapid and sensitive methods using visible spectrophotometry and HPLC are described for the determination of frusemide (FRU) in bulk drug and formulations. Spectrophotometry is based on a redox reaction involving FRU followed by complexation reaction and uses iron(iii) and ferricyanide(iii) as reagents. The resulting Prussian blue is measured at 760 nm. The HPLC determination was carried out on a reversed phase Accurasil ODS C 18 column ( mm, 5 μm) using a mobile phase consisting of acetonitrile 0.1% orthophosphoric acid (ph 3) ( ) at a flow rate of 1.0 ml min -1 with UV detection at 233 nm. Working conditions of both methods have been optimized and the methods validated as per the ICH guidelines. In spectrophotometry, a regression analysis of Beer s law plot showed a good correlation in the concentration range μg ml -1 with an apparent molar absorptivity of L mol -1 cm -1 and a Sandell sensitivity of 7.85 ng cm -2. The limits of detection and quantification were calculated to be 0.09 and 0.28 μg ml -1 respectively. The linear range of determination by HPLC was μg ml -1. The detection limit (S/N = 3) and quantification limit (S/N = 10) were found to be 0.3 and 0.6 μg ml -1, respectively. Within-day accuracies and precisions were 3% and between-days precisions were less than 5% for all the concentrations tested. The methods were applied to the assay of FRU in tablets and injections. The label claim percentages and relative standard deviations were in the and % range, respectively. The validity of the methods was further ascertained by parallel determination by a reference method and by recovery studies via standard addition technique. The results showed that the procedures are suitable for routine analysis of the diuretic. Keywords: Frusemide, assay, spectrophotometry, HPLC, prussian blue, formulations IPC Code:B01D15/00; A61K Frusemide (FRU), is chemically benzoic acid-5- (aminosulphonyl)-4-chloro-2-[2-furanylmethyl) amino)-4-cholro-n furfuryl-5-sulfamoyl anthranilic acid. In chemotherapy, it is extensively used for the treatment of edema associated with pulmonary, cardiac, hepatic, and renal disease 1, and hypertension accompanied by fluid retention or impaired renal failure 2. Numerous analytical methods are available for the assay of FRU in pharmaceutical samples. Spectrophotometric methods based on different reaction schemes have been reported for the assay of FRU. The method developed by Kustrin et al. 3 uses palladium(ii) as complexing agent, but lacks sensitivity (linear range, μg ml -1 ). Procedures based on binary complex formation reaction with iron(iii) 4 and ternary complex formation reaction 5 with iron(iii) and thiocyanate are least *For correspondence ( basavaiahk@yahoo.co.in; Fax: ) sensitive with the linear range of applicability being mg ml -1. Spectrophotometric methods utilizing redox 6, oxidative coupling 7,8 and diazo coupling 9 reactions also involve a heating step for a quantitative reaction. Ion-pair complex formation 10,11 and derivatisation 12 reactions based procedures involve an extraction step in addition to using organic solvents. Also, the procedures are poorly sensitive ( 10 3 ) and require a heating step 12. The determination of FRU based on charge transfer complex formation reaction, although sensitive, has to be performed in acetonitrile medium 13. Of the various techniques used in pharmaceutical analysis, HPLC is one of the most sensitive and rapid. However, the number of methods based on this technique for the say of FRU in pharmaceutical samples is limited. Rapaka et al. 14,15 were the first to report the HPLC determination of FRU in bulk material and pharmaceutical samples. The determination of FRU upto 75 μg ml -1 has been
2 402 INDIAN J. CHEM. TECHNOL., JULY 2005 achieved by Rao and Raghuveer 16 on a micro Bondapak NH 2 column with UV-detection at 254 nm and methanolic 0.01 M sodium acetate of ph 7.8 as mobile phase. The limit of detection was 0.5 μg ml -1. One of the most sensitive HPLC methods for FRU has been accomplished with an electrochemical detection 17. The method employed ODS material as the analytical column with methanol 0.04 M KH 2 PO 4 of ph 2.0 (1:1) as the mobile phase and detection at V versus Ag-AgCl electrode. The calibration graph was rectilinear from 1 to 10 μg ml -1 and the detection limit was 0.5 μg ml -1 which is a 10-fold improvement compared to UV-detection. This paper describes two methods based on visible spectrophotometry and reversed phase HPLC techniques for the assay of FRU in pharmaceutical formulations containing FRU alone. Spectrophotometry is based on the formation of Prussian blue when the drug is treated with iron(iii) chloride and ferricyanide (III) and measurement of the resulting blue colour at 760 nm. The HPLC analysis was carried out by injecting the drug solution onto an Accurasil ODS column with the elution being effected by a mobile phase consisting of acetonitrile and 0.1% orthophosphoric acid of ph 3 (60+40) and UV detection at 233 nm. The spectrophotometric method is the most sensitive ever reported and the HPLC procedure is the most sensitive with UV detection for FRU in single dosage forms. Both methods were found to be selective, accurate and precise. Experimental Procedure Apparatus A systronics model 106 digital spectrophotometer with 1 cm matched quartz cells was used for absorbance measurements. The chromatographic system consisted of an Agilent 1100 series chromatograph equipped with an inbuilt solvent degasser, quaternary pump, photodiode assay detector with variable injector and autoanalyzer. A steel column ( mm) of Accurasil ODS C 18 (5 μm) was used. Reagents and materials All reagents used were of analytical reagent grade and distilled water was used to prepare solutions for spectrophotometric work. For HPLC work, distilled water filtered through 0.45 μm filter was used to prepare solutions. A 0.2% aqueous solution of iron(iii) chloride was prepared afresh every time before use by dissolving 0.2 g of the chemical in 100 ml of water and stored in an amber coloured bottle. A 0.4% aqueous solution of potassium ferricyanide was prepared by dissolving 0.4 g of the reagent in 100 ml of water. Sulphuric acid (10 M) was prepared by adding 55 ml of concentrated acid to 45 ml of water with cooling. Orthophosphoric acid (0.1%) was prepared by diluting 1 ml of the acid to one litre with distilled water and filtered through 0.45 μm filter. The mobile phase used in HPLC consisted of HPLC grade acetonitril and 0.1% H 3 PO 4 of ph 3 in the ratio 60:40. The dilutent solution was prepared by mixing acetonitrile and water in the ratio 60:40. Pharmaceutical grade frusemide was received as gift from Geno Pharmaceuticals Ltd., Mumbai, India. Formulations containing FRU were purchased from local commercial sources. A stock standard solution equivalent to 200 μg ml -1 FRU was prepared by dissolving 20 mg of pure drug in 20 ml of methanol and diluting to the mark with water in a 100 ml calibrated flask. This was diluted 10-fold with water and used in spectrophotometric analysis. For assay by HPLC, a stock standard solution containing 203 μg ml -1 FRU was prepared by dissolving 20.3 mg of pure drug in diluent solution and diluting to the mark in a 100 ml calibrated flask. Methods Spectrophotometric method Varying aliquots ( ml) of 20 μg ml -1 FRU solution were transferred into a series of 10 ml calibrated flasks by means of a microburette and the volume was brought to 2 ml by adding water. To each flask was then added 2 ml of 0.2% iron(iii) chloride solution followed by 1 ml of 0.4% ferricyanide solution. The contents were mixed well and let stand for 20 min with occasional shaking. Finally, 1 ml of 10 mol L -1 H 2 SO 4 was added and diluted to the mark with water, and absorbance was measured at 760 nm against a reagent blank. A calibration graph was prepared by plotting the absorbance versus concentration of drug. The concentration of the unknown was read from the calibration graph or computed from the regression equation derived from the Beer s law data. HPLC Method Chromatographic conditions The chromatographic separation was achieved at ambient temperature on a reversed phase Accurasil ODS 5 μm C 18 column using a mobile phase consisting of acetonitrile-0.1% H 3 PO 4 of ph 3
3 BASAVAIAH & CHANDRASHEKAR: ANALYSIS OF FRUSEMIDE BY SPECTROPHOTOMETRY & HPLC 403 (60+40) at a flow rate of 1.0 ml min -1. The detector wavelength was set at 233 nm with a sensitivity of 0.2 a.u.f.s. Preparation of calibration graph Working standard solutions containing μg ml -1 FRU were prepared by appropriate dilution of the stock standard solution with the diluent solution. Twenty μl aliquot of each solution was injected automatically onto the column in duplicate and the chromatograms were recorded. Calibration graph was constructed by plotting the mean peak area versus concentration of FRU. The concentration of the unknown was read from the calibration graph or calculated with the help of regression equation derived from the peak areaconcentration data. Assay procedure for formulations Twenty tablets were weighed and ground into a fine powder with agate pestle and mortar. The contents of ten ampoules were emptied into a beaker and mixed. An accurately weighed amount of the tablet powder equivalent to 20 mg of FRU was transferred into a 100 ml calibrated flask, 20 ml of methanol was added and shaken for 20 min. The mixture was diluted to the mark with water and mixed well, and filtered using a Whatman No. 42 filter paper. First 10 ml portion of the filtrate was discarded and subsequent 10 ml portion was diluted to 100 ml with water in a calibrated flask. A suitable aliquot of this solution (20 μg ml -1 FRU) was subjected to analysis by spectrophotometry. An accurately measured aliquot of the injectable product equivalent to 20 mg of FRU was transferred into a 100 ml calibrated flask, 20 ml of methanol added and diluted to the mark with water. This solution was diluted 10-fold and the convenient aliquot of the diluted solution was then analysed spectrophotometrically as described previously. An amount of tablet powder equivalent to 20 mg of FRU was accurately transferred into a 100 ml calibrated flask, 60 ml diluent solution added and shaken for 20 min. The mixture was diluted to the mark with the diluent and mixed well. A small portion of this (~ 10 ml) was withdrawn and filtered through a 0.2 μm filter to ensure the absence of particulate matter. The filtered solution was appropriately diluted to a convenient working concentration and 20 μl aliquot was then injected to get the chromatogram. In the case of injection, a measured aliquot equivalent to 20 mg of FRU was diluted to 100 ml with the diluent and mixed well. A 10 ml aliquot of this solution was filtered through a 0.45 μm filter. A suitable aliquot of the filtrate was appropriately diluted before analysis as described for tablets. Results and Discussion Spectrophotometry Primary and secondary amines reduce ferric ferricyanide to Prussian blue 18 and this reaction has been successfully used for the spectrophotometric determination of several pharmaceuticals FRU which contains both primary and secondary amino groups reduces ferric ferricyanide to the blue colour which could be measured at 760 nm and related to the drug concentration. The optimum experimental conditions were established by varying one parameter at a time and observing its effect on the absorbance of the coloured species. Two ml of 0.2% iron(iii) chloride and 1 ml of 0.4% ferricyanide solutions in a total volume of 10 ml were found to produce maximum sensitivity in the concentration range investigated. The blue colour formed following the reduction of ferric ferricyanide was found to flocculate within min. However, addition of 1 ml of 10 mol L -1 H 2 SO 4 after full colour development but before diluting to the mark was found to delay flocculation and increase the stability of colour. The reduction reaction was found to be slow at room temperature (30 ± 2 C) but the absorbance increased with time and reached maximum in 20 min. The developed colour was stable for at least 90 min. After fixing all other parameters, experiments were performed to study the effect of order of addition reactants. The order, FRU: iron(iii): ferricyanide followed by sulphuric acid after full colour development gave maximum sensitivity and stability of coloured species and hence followed throughout the investigation. HPLC method FRU was determined by HPLC by injecting the solution onto an Accurasil ODS 5 μm column with UV detector set at 233 nm. No internal standard was used. The composition and ph of the mobile phase and its flow rate were varied to optimize the chromatographic conditions. A mobile phase consisting of acetonitrile and 0:1% H 3 PO 4 of ph 3 was selected after several preliminary experiments with acetonitrile-water and methanol-water. Acetonitrile and phosphoric acid increase the
4 404 INDIAN J. CHEM. TECHNOL., JULY 2005 solubility of FRU and prevent its adherence to the packing material in the column. At a flow rate of 1.0 ml min -1, the retention time for FRU was 3.05 min. Under the described experimental conditions the analyte peak was well defined and free from tailing. Method validation Spectrophotometric method The increasing absorbance values at 760 nm were plotted against the drug concentration to obtain the calibration graph. Beer s law is obeyed over the concentration range μg ml -1 the equation of the line being, A = C where A is the absorbance and C concentration in μg ml -1. The correlation coefficient of the calibration graph was calculated to be (n=8) confirming a linear increase in absorbance with increasing concentration of FRU. The calculated molar absorptivity was L mol -1 cm -1 and the Sandell sensitivity was 7.85 ng cm -2. The limits of detection (LOD) and quantification (LOQ) were calculated from the standard deviation of the absorbance measurement obtained from a series of seven blank solutions. The LOD and LOQ established according to ICH guidelines 26 were 0.09 and 0.28 μg ml -1, respectively. HPLC Method The concentration of the unknown was determined by measuring the peak area. A plot of mean peak area versus concentration gave a linear relationship (r= ) over the concentration range μg ml -1. Using the method of least squares, the linear regression equation obtained was Y = X where Y is the mean peak area and X concentration in μg ml -1. The LOD was established at a signal to noise ratio (S/N) of 3 and LOQ was established at a S/N ratio of 9. The LOD was calculated to be 0.3 μg ml -1 and the LOQ was calculated to be 0.6 μg ml -1. Precision and accuracy of the methods The within-day precision of the methods was determined by replicate analyses of the standard solution containing FRU at three different concentration levels and the results are presented in Tables 1 and 2. For HPLC method, the relative standard deviation (RSD) which is a measure of precision was evaluated for both retention time and peak area. The inter-day precision was established by performing analysis over a 5 days period with solutions prepared freshly each day. The RSD values were not more than 4 and 5% for spectrophotometric and HPLC methods, respectively. The peak area based and retention time based inter-day RSD values were 0.8 and 4.5%, respectively. In order to demonstrate the accuracy of the proposed methods, seven replicate analyses were performed on solutions containing FRU at three different levels. The results obtained are compiled in Tables 1 and 2 and the accuracy expressed as percent relative error was found satisfactory. Application Commercially available tablets and injections containing FRU were analysed by the described spectrophotometric and HPLC methods. The results obtained are summarized in Table 3. As can be seen, the results are in agreement with the labelled amounts. For comparison, a conventional UV-absorption method ( λ = 275 nm) due to Moustafa and Abdel Moety 27 was used to analyse the same batch tablets Table 1 Evaluation of accuracy and precision (spectrophotometry) FRU taken μg ml -1 FRU found* μg ml -1 Range μg ml -1 RE RSD, % ROE ± ± ±2.65 * Mean value of seven determinations, RE: Relative Error, RSD: Relative standard deviation, ROE: Range of error at 95% confidence level Table 2 Evaluation of accuracy and precision (HPLC) FRU taken μg ml -1 FRU found μg ml -1 Range, μg ml -1 RE, RSD % ROE % RSD** ROE** *Based on peak area, ** Based on retention time
5 BASAVAIAH & CHANDRASHEKAR: ANALYSIS OF FRUSEMIDE BY SPECTROPHOTOMETRY & HPLC 405 Formulation and Brand name * Frusenex a tablets Lari b Injections Table 3 Results of analysis of formulations containing frusemide Nominal amount mg/tablet or mg/ml Found ** (% of nominal amount ± SD) Reference method Spectrophotometric method HPLC method ± ±1.62 t= 0.86 F= ± ±2.05 t= 0.42 F= ± ±1.85 t= 1.00 F=5.92 * Marketed by : a. Geno Pharm Ltd, : b. Indian Drugs and Pharmaceuticals Ltd. ** Mean value of five determinations Tabulated t-value at 95% confidence level in 2.77 Tabulated t-value at 95% confidence level in 6.39 Table 4 Results of recovery experiments Formulation studied Frusenex tablets (40 mg) Drug in formulation μg ml -1 Spectrophotometric method Pure drug added, μg ml -1 Total found μg ml -1 Recovery* of pure drug added, % Drug in formulation μg ml -1 HPLC method Pure drug added, μg ml -1 Total found μgml ±0.44 t= 2.45 F= ±0.62 t= 2.00 F= ±0.36 t= 2.31 F=4.45 Recovery*of pure drug added, % Lasix injections (10 mg /ml) *Mean value of three determinations and injections. The results, compiled in Table 3 were statistically compared by Student s t-test and F-test. As shown, the calculated t - and F - values were less than the tabulated values inferring that the proposed methods have the same accuracy and precision as that of the reference method at the 95% confidence level. The accuracy and reliability of the methods were further established by performing recovery experiments. The pre-analysed tablets and injections were spiked with pure FRU at three different levels and the total was found by the proposed methods. Each experiment was repeated three times. The recoveries of the added pure drug were in the range % (Table 4) indicating that coformulated substances such as talc, starch, gumacacia, lactose, magnesium stearate, sodium alginate, calcium gluconate, and calcium dihydrogenorthophosphate did not interfere in the determination. Conclusions A simple and highly sensitive spectrophotometric method based on a well known reduction - complexation reaction is reported. The procedure does not involve either a heating or extraction step unlike many reported previously. The present HPLC method is superior to many reported earlier in terms of sensitivity, linear range of response, and analysis time (6 min). In terms of limit of detection, the proposed method is next only to the procedure employing electrochemical detector 17. Acknowledgements The authors gratefully acknowledge the receipt of pure frusemide from Geno Pharmaceuticals Ltd., Mumbai as gift. One of the authors (UC) thanks the authorities of the University of Mysore, Mysore for facilities.
6 406 INDIAN J. CHEM. TECHNOL., JULY 2005 References 1 Delgado J N & Remers W A (eds), in Wilson and Gisvold s Text Book of Organic Medicinal and Pharmaceutical Chemistry (J.B. Lippincott Co., Philadelphia, P.A), 1991, Foye W O (ed), Principles of Medicinal Chemistry (Lea and Febiger, Philadelphia, PA), 1989, Agatonovic Kurstin S, Zivanovic L, Radulovic D & Pecanac D, J Pharm Biomed Anal, 8 (1990) Zivanovic L, Agatonovic S & Radulovic D, Mikrochim Acta, I (1-2) (1990) Zivanovic L, Agatonovic Kurstrin S & Radulovic D, Pharmazie, 45 (1990) Issopoulos P B, Fresenius Z Anal Chem, 334 (1989) Sastry C S P, Suryanarayana M V & Tipirneni A S R P, Talanta, 36 (1989) Sastry C S P, Prasad T N V, Sastry B S & Rao E V, Analyst (London), 113 (1988) Rao N V, Murthy R V V S, Rao S N, Chandrashekharan K S & Mythili P, J Inst Chem (India), 59 (1987) Prasad T N V, Sastry B V, Rao E V & Sastry C S P, Pharmazie, 42 (1987) Sastry C S P, Suryanarayana M V, Tipirneni A S R P, Sastry B S, Indian Drugs, 26 (1989) Sevillano - Cabeza A, Campins-Falco P & Serrador-Garcia M C, Anal Lett, 30 (1997) Mohamed A M I, J Assoc Off Anal Chem, 72 (1989) Rapaka R S, Roth J & Prasad V K, Int J Pharm, 11 (1982) Roth J, Rapaka R S & Prasad V K, Anal Lett, 14 (1981) Rao G K & Raghuveer S, Indian Drugs, 22 (1985) Kamata K, Takahashi M, Uehara S, Hagiwara T, Nakayama K, Akiyama K & Nishigaki S, Iyakuhin Kenkyu, 19 (1988) Pesez M & Bartos J, Ann Pharm Fr, 23 (1965) Basavaiah K & Prameela H C, Indian Drugs, 39 (2002) Basavaiah K, Chandrashekar U & Charan V S, East Pharm, 44 (2001) Basavaiah K & Prameela H C, Science Asia, 29 (2003) Basavaiah K, Chandrashekar U & Prameela H C, IL Farmaco, 58 (2003) Murthy T K, Reddy M N, Srinivasa Rao Y, Srinivasan K & Sankar D G, Asian J Chem, 13 (2001) Reddy M N, Krishnamurthy T, Rajita K & Sankar D G, Indian J Pharm Sci, 63 (2001) Sheshagiri Rao J V L N, Srinivasa Rao Y, Murthy T K & Sankar D G, Asian J Chem, 14 (2002) ICH Steering Committee, Text on Validation of Analytical Procedures, International Conference on Harmonization of Technical Requirements For Registration of Pharmaceuticals For Human Use, Moustafa A A & Abdel Moety E M, Farmaco ed Prat, 42 (1987) 51.
A NEW HPLC METHOD FOR THE QUANTIFICATION OF PANTOPRAZOLE IN PHARMACEUTICALS
Int. J. Chem. Sci.: 6(2), 2008, 579-586 A NEW HPLC METHOD FOR THE QUANTIFICATION OF PANTOPRAZOLE IN PHARMACEUTICALS K. BASAVAIAH, U. R. ANIL KUMAR and K. THARAPA Department of Chemistry, University of
More informationZero And First Order Derivative Spectrophotometric Methods For Determination Of Dronedarone In Pharmaceutical Formulation
International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.1, pp 217-221, Jan-Mar 2013 Zero And First rder Derivative Spectrophotometric Methods For Determination f Dronedarone
More informationInternational Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010
International Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010 RP HPLC ESTIMATION OF EZETIMIBE IN TABLET DOSAGE FORMS NAGARAJU. P *, KRISHNACHAITHANYA. K, CHANDRABABU. D, SRINIVAS.
More informationStability-indicating HPLC determination of tolterodine tartrate in pharmaceutical dosage form
Indian Journal of Chemical Technology Vol. 13, May 2006, pp. 242-246 Stability-indicating HPLC determination of tolterodine tartrate in pharmaceutical dosage form Vinay Saxena a *, Zahid Zaheer b & Mazhar
More informationUV Spectrophotometric Estimation of Levoceterizine Dihydrochloride in Bulk and Dosage Form
http://www.e-journals.net ISSN: 0973-4945; CODEN ECJHAO E- Chemistry 2010, 7(S1), S414-S418 UV Spectrophotometric Estimation of Levoceterizine Dihydrochloride in Bulk and Dosage Form P. MAMATHA, P. V.ANANTHA
More informationValidated First Order Derivative Spectroscopic Method for the determination of Stavudine in Bulk and Pharmaceutical Dosage Forms
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.3, No.1, pp 18-22, Jan-Mar 2011 Validated First Order Derivative Spectroscopic Method for the determination of Stavudine
More informationA RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF PARA- PHENYLENEDIAMINE IN PURE FORM AND IN MARKETED PRODUCTS
A RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF PARA- PHENYLENEDIAMINE IN PURE FORM AND IN MARKETED PRODUCTS CH.MOUNIKA*, M.KINNERA Research Article SIR.C.R.REDDY COLLEGE OF PHARMACEUTICAL SCIENCES, ELURU.
More informationStability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage form
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Stability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage form
More informationDETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
CHAPTER 9 DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 9 Determination of drug release during
More informationChapter-4 EXPERIMENTAL WORK BY RP-HPLC
Chapter-4 EXPERIMENTAL WORK BY RP-HPLC 4.0 EXPERIMENTAL WORK BY RP-HPLC 4.1. DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR THE DETERMINATION OF NIFLUMIC ACID 4.1.1. MATERIALS AND METHODS OF NIFLUMIC
More informationVALIDATION STUDY OF THE HPLC ASSAY OF SOME ANTIAMOEBIC AGENTS
J. Curr. Chem. Pharm. Sc.: 2(3), 2012, 191-197 ISSN 2277-2871 VALIDATION STUDY OF THE HPLC ASSAY OF SOME ANTIAMOEBIC AGENTS N. D. DINESH * and K. S. RANGAPPA a Department of Chemistry, Adichunchanagiri
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(1): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(1):118-122 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of High Performance Liquid
More informationJournal of Pharmaceutical and Biomedical Analysis Letters. Analysis Letters
Naga Jyothi. C et al, JPBMAL, 2015, 3(1): 242 246 ISSN: 2347-4742 Journal of Pharmaceutical and Biomedical Analysis Letters Journal Home Page: www.pharmaresearchlibrary.com/jpbmal Research Article Open
More informationInternational Journal of Pharma and Bio Sciences
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACEUTICAL ANALYSIS DEVELOPMENT, ESTIMATION AND VALIDATION OF BOSENTAN IN BULK AND IN ITS PHARMACEUTICAL FORMULATION BY UV-VIS SPECTROSCOPIC
More informationInternational Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: , ISSN(Online): Vol.9, No.7, pp , 2016
International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 097-30, ISSN(Online): 255-9563 Vol.9, No.7, pp 399-06, 2016 Analytical Quality by Design Approach for Development of UV-Spectrophotometric
More informationCHAPTER 19 PARACETAMOL + IBUPROFEN
CHAPTER 19 PARACETAMOL + IBUPROFEN SUMMARY 246 A combination of paracetamol and ibuprofen is marketed in India. Literature survey indicated that one titrimetnc-1-** and one HPLC193 methods are reported
More informationNEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF OXOLAMINE IN FORMULATION
NEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF OXOLAMINE IN FORMULATION V.PhaniKumar 1 *, CH.Venkata Kishore 2 1 Department of Chemistry, Govt college, Tiruvuru, Krishna District Andhra
More informationA Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation
Pharmaceutical Research A Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation Manisha Puranik* a, Sailesh Wadher b and Kritika
More informationChapter 4: Verification of compendial methods
Chapter 4: Verification of compendial methods Introduction In order to ensure accurate and reliable test results, the quality control laboratory (QCL) needs to use analytical methods (and accompanying
More informationImpact factor: 3.958/ICV: 4.10 ISSN:
Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 99 Pharma Science Monitor 9(4), Oct-Dec 2018 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com
More informationEstimation of Dapagliflozin from its Tablet Formulation by UV-Spectrophotometry
Pharm Methods, 2017; 8(2): 102-107 A multifaceted peer reviewed journal in the field of Pharm Analysis and Pharmaceutics www.phmethods.net www.journalonweb.com/phm Original Article Estimation of Dapagliflozin
More informationValidated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form
International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.3, pp 1228-1232, July-Sept 2012 Validated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form Manzoor
More informationRP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.ejchem.net 2012, 9(3), 1407-1411 RP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms M. VIJAYA LAKSHMI 1, J.V.L.N.S. RAO 2 AND A. LAKSHMANA
More informationCHAPTER - 3 ANALYTICAL PROFILE. 3.1 Estimation of Drug in Pharmaceutical Formulation Estimation of Drugs
CHAPTER - 3 ANALYTICAL PROFILE 3.1 Estimation of Drug in Pharmaceutical Formulation 3.1.1 Estimation of Drugs ANALYTICAL PROFILE 84 3.1 ESTIMATION OF DRUG IN PHARMACEUTICAL FORMULATION. Agrawal A et al
More informationResearch Article Spectrophotometric Estimation of Didanosine in Bulk Drug and its Formulation
Research Article Spectrophotometric Estimation of Didanosine in Bulk Drug and its Formulation RN. Kane, PS. Bhokare*, CC. Nalawade, MS Sayyed and RD. Paliwal Department of Pharmaceutical Chemistry, Singhad
More informationSimultaneous Estimation of Metolazone and Spironolactone in Combined Tablet Dosage Form BY UV Spectroscopy.
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.3, No.4, pp 2068-2074, Oct-Dec 2011 Simultaneous Estimation of Metolazone and Spironolactone in Combined Tablet Dosage
More informationINTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES
International Journal of Institutional Pharmacy and Life Sciences 4(2): March-April 2014 INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES Pharmaceutical Sciences Original Article!!! Received:
More informationAnalytical method development and validation of gabapentin in bulk and tablet dosage form by using UV spectroscopic method
IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: www.ijpar.com ISSN:2320-2831 Research article Open Access Analytical method development and validation of gabapentin in bulk and tablet dosage
More informationMethod development and validation for the estimation of metronidazole in tablet dosage form by UV spectroscopy and derivative spectroscopy
IJPAR Volume 3 Issue 2 April-June-2014 ISSN: 2320-2831 Available Online at: www.ijpar.com [Research article] Method development and validation for the estimation of metronidazole in tablet dosage form
More informationA Simple, Sensitive Spectrophotometric Determination of Mosapride in Pharmaceutical Preparations Using Novel Reagent
ISS: 0973-4945; CODE ECJHAO E- Chemistry http://www.e-journal.net Vol. 1, o. 5, pp 267-271, October 2004 A Simple, Sensitive Spectrophotometric Determination of Mosapride in Pharmaceutical Preparations
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD TO DETERMINE CINITAPRIDE HYDROGEN TARTARATE IN BULK AND PHARMACEUTICAL FORMULATION
Research Article ISSN:2230-7346 Journal of Global Trends in Pharmaceutical Sciences Vol.3, Issue 2, pp -619-627, April June 2012 DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD TO DETERMINE CINITAPRIDE HYDROGEN
More informationDevelopment and validation a RP-HPLC method: Application for the quantitative determination of quetiapine fumarate from marketed bulk tablets
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(1):142-146 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and validation a RP-HPLC method: Application
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF GABAPENTIN IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 10(4), 2012, 2209-2217 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF GABAPENTIN IN PURE AND PHARMACEUTICAL FORMULATIONS
More informationRevision Bulletin 29 Dec Jan 2018 Non-Botanical Dietary Supplements Compliance
Niacin Extended-Release Tablets Type of Posting Posting Date Official Date Expert Committee Reason for Revision Revision Bulletin 29 Dec 2017 01 Jan 2018 Non-Botanical Dietary Supplements Compliance In
More informationINTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND LIFE SCIENCES
A. Chenthilnathan et al IJRPLS, 2014, 2(2): 185-190 Research Article Available online at www.pharmaresearchlibrary.com/ijrpls ISSN: 2321-5038 INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND LIFE SCIENCES
More informationReverse Phase High Performance Liquid Chromatography method for determination of Lercanidipine hydrochloride in bulk and tablet dosage form
Research Article ISSN: 0974-6943 M.V.Kumudhavalli et al. / Journal of Pharmacy Research 2014,8(11), Available online through http://jprsolutions.info Reverse Phase High Performance Liquid Chromatography
More informationINTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE
INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE DEVELOPMENT OF VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF BETAXOLOL HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM SIDHARTH
More informationNEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DESLORATADINE IN PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 10(4), 2012, 2067-2072 ISSN 0972-768X www.sadgurupublications.com NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DESLORATADINE IN PHARMACEUTICAL FORMULATIONS CH. CHANDRA SEKHAR
More informationINTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES
International Journal of Universal Pharmacy and Bio Sciences 2(4): July-August 2013 INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES IMPACT FACTOR 1.89*** ICV 3.00*** Pharmaceutical Sciences
More informationRevision Bulletin 27 Jan Feb 2017 Non-Botanical Dietary Supplements Compliance
Niacin Extended-Release Tablets Type of Posting Posting Date Official Date Expert Committee Reason for Revision Revision Bulletin 27 Jan 2017 01 Feb 2017 Non-Botanical Dietary Supplements Compliance In
More informationDevelopment and Statistical Validation of Spectrophotometric Methods for the Estimation of Nabumetone in Tablet Dosage Form
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2010, 7(4), 1463-1467 Development and Statistical Validation of Spectrophotometric Methods for the Estimation of Nabumetone in Tablet
More informationVolume 6, Issue 2, January February 2011; Article-015
Research Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE DETERMINATION OF DAPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION USING AN EXPERIMENTAL DESIGN Pratik Mehta*, Ujjwal Sahoo,
More informationJournal of Chemical and Pharmaceutical Research, 2012, 4(6): Research Article. Estimation of zaleplon by a new RP-HPLC method
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(6):3010-3014 Research Article ISS : 0975-7384 CODE(USA) : JCPRC5 Estimation of zaleplon by a new RP-HPLC method Tentu.
More informationDevelopment of Validated Analytical Method of Mefenamic Acid in an Emulgel (Topical Formulation)
Development of Validated Analytical Method of Mefenamic Acid in an Emulgel (Topical Formulation) TEENA OSWAL*, DR.SURYAKANT BHOSALE, DR. SONALI NAIK MET Institute Of Pharmacy Met Complex, Bandra Reclamation,
More informationAnalytical method development and validation of carvedilol in bulk and tablet dosage form by using uv spectroscopic method as per ich guidelines
IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: ISSN:2320-2831 Research article Open Access Analytical method development and validation of carvedilol in bulk and tablet dosage form by using
More informationDEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION
Page186 Research Article Pharmaceutical Sciences DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION Kishore Konam 1 &
More informationSensitive Spectrophotometric Method for the Determination of Prazosin
Available online at www.ijacskros.com Sensitive Spectrophotometric Method for the Determination of Prazosin G. Dilli Rani 1, C. Narasimha Rao 1, C. Narasimha Rao 2, A. Narayana 3, P. Venkateswarlu 1 *
More informationSpectrophotometric estimation and validation of hydrochlorothiazide in tablet dosage forms by using different solvents
Available online at www.derpharmachemica.com Scholars Research Library Der Pharma Chemica, 2012, 4 (1):10-14 (http://derpharmachemica.com/archive.html) ISSN 0975-413X CODEN (USA): PCHHAX Spectrophotometric
More information7. Stability indicating analytical method development and validation of Ramipril and Amlodipine in capsule dosage form by HPLC.
7. Stability indicating analytical method development and validation of and in capsule dosage form by HPLC. 7.1 INSTRUMENTS AND MATERIALS USED 7.1.1 INSTRUMENTS 1. Shimadzu LC-2010 CHT with liquid chromatograph
More informationReceived: ; Accepted:
International Journal of Universal Pharmacy and Bio Sciences 1(2): November-December2012 INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES Pharmaceutical Sciences Research Article!!! Received:
More informationCu-Creatinine- Metol system
Quantification of Creatinine in Human Serum using Metol as a Chromogenic Probe Materials and methods 6.1. Reagents 6.1.1. N-methyl-p-aminophenol sulfate N-methyl-p-aminophenol sulfate also denoted as Metol
More informationKetorolac tromethamine (KT)[1] is
RESEARCH ARTICLE Derivative and Simultaneous Equation Methods for the Determination of Fluorometholone and Ketorolac in Ophthalmic Preparations Chandaka Prasanna Kumar*, Bulusu Ravi Teja, Bhupathiraju
More informationSTABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG AND IT S PHARMACEUTICAL DOSAGE FORM
International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 3, Issue 3, 2011 Research Article STABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG
More informationRP-HPLC Method for Simultaneous Estimation of Atenolol, Hydrochlorothiazide and Losarton in Tablet Dosage form
Chem Sci Trans., 2013, 2(S1), S1-S6 Chemical Science Transactions DOI:10.7598/cst2013.296 ISS/E-ISS: 2278-3458/2278-3318 RESEARCH ARTICLE RP-HPLC Method for Simultaneous Estimation of Atenolol, Hydrochlorothiazide
More informationNEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF PARACETAMOL IN PURE FORM AND PHARMACEUTICAL FORMULATIONS
NEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF PARACETAMOL IN PURE FORM AND PHARMACEUTICAL FORMULATIONS Siva lokesh.b, Uma maheswari.t, Anusha.B, Ramya.B, Sri Sai Rohini.T, Sudheerbabu.I* Sir
More informationof nm throughout the experimental work.
Difference Spectrophotometric Methods for Pioglitazone Hydrochloride and Metformin Hydrochloride K.Sujana, K.Abbulu, O.Bala Souri, B.Archana, M.Sindu, G.Swathi Rani Department of Pharmaceutical Analysis,
More informationEstimation of Fenofibric Acid in Pharmaceutical Oral Solid Dosage Form by UV-Spectrophotometry
Human Journals Research Article March 2015 Vol.:2, Issue:4 All rights are reserved by V. NIRAIMATHI et al. Estimation of Fenofibric Acid in Pharmaceutical Oral Solid Dosage Form by UV-Spectrophotometry
More information462 1 & (2&3 ( 4 5 6" 6 7 ' ("0 / L1 1 % FG &
. 2009 *!" % & ' #$. %* () 1 () -6-"/ (0 42. / & ' :', 462 1 & (2&3 ( 4 5 6" 6 7 ' ("0 / () 0?
More informationRP-HPLC Method Development and Validation of Dapagliflozin in Bulk and Tablet formulation
221 IJPAR Volume 2 Issue 4 Oct - Dec -2013 ISSN: 2320-2831 Available Online at: [Research article] RP-HPLC Method Development and Validation of Dapagliflozin in Bulk and Tablet formulation Jeyabaskaran.M
More informationLiquid Chromatography and Spectroscopic Method for Estimation of Sulphadoxine in Marketed Formulation
Human Journals Research Article August 2018 Vol.:13, Issue:1 All rights are reserved by A. Yasodha et al. Liquid Chromatography and Spectroscopic Method for Estimation of Sulphadoxine in Marketed Formulation
More informationThe Nitrofurantoin Capsules Revision Bulletin supersedes the currently official monograph.
Nitrofurantoin Capsules Type of Posting Revision Bulletin Posting Date 25 May 2018 Official Date 01 Jun 2018 Expert Committee Chemical Medicines Monographs 1 Reason for Revision Compliance In accordance
More informationJ Pharm Sci Bioscientific Res (4): ISSN NO
Development and Validation of Stability Indicating Analytical Method for Simultaneous Estimation of Perindopril and Potassium in Their Combined Marketed Dosage Form ABSTRACT: Gurjeet Kaur*, Nikhil Patel
More informationUV Spectrophotometric Method Development and Validation of Ezetimibe and Simvastatin in Bulk and Pharmaceutical Dosage Form
ISSN 2395-3411 Available online at www.ijpacr.com 581 Research Article UV Spectrophotometric Method Development and Validation of Ezetimibe and Simvastatin in Bulk and Pharmaceutical Dosage Form Namratha
More informationInternational Journal of Pharma and Bio Sciences V1(1)2010 UV- SPECTROPHOTOMETRIC DETERMINATION OF TENATOPRAZOLE FROM ITS BULK AND TABLETS
M. SUGUMARAN*, R.NAGESWARA RAO AND D. JOTHIESWARI Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur- 603319,Tamilnadu,India. * Corresponding author murugesansugumaran@yahoo.com
More informationPelagia Research Library. Spectrophotometric determination of Ametoctradin and in its commercial formulations
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2016, 7(4):7-14 ISSN: 0976-8688 CODEN (USA): PSHIBD Spectrophotometric determination of Ametoctradin and in its commercial formulations
More informationAREA UNDER CURVE AND SECOND ORDER DERIVATIVE SPECTROSCOPY OF METAXALONE IN BULK DRUG AND TABLET FORMULATION
Int. J. Chem. Sci.: 8(2), 2010, 823-827 AREA UNDER CURVE AND SECND RDER DERIVATIVE SPECTRSCPY F METAXALNE IN BULK DRUG AND TABLET FRMULATIN J. PRIYADHARISINI, G. P. GIGI, V. NIRAIMATHI and A. JERAD SURESH
More informationJournal of Global Trends in Pharmaceutical Sciences
An Elsevier Indexed Journal ISSN-2230-7346 Journal of Global Trends in Pharmaceutical Sciences VALIDATED COLORIMETRIC METHOD FOR THE ESTIMATION OF SUCCINYL CHOLINE CHLORIDE IN FORMULATION K. Swaroopa Rani*,
More informationSimultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(4), 1620-1625 Simultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium DESHMUKH HAFSA, S. CHANDA
More informationDepartment of Quality Assurance, Luqman College of Pharmacy, GULBARGA (K.S.) INDIA ABSTRACT
Int. J. Chem. Sci.: 12(3), 2014, 871-879 ISSN 0972-768X www.sadgurupublications.com DEVELPMENT AND VALIDATIN F A RAPID RP HPLC METHD FR THE DETERMINATIN F CINITAPRIDE HYDRGEN TARTARATE IN PURE AND ITS
More informationDevelopment and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method
21 Article Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method Mali Audumbar Digambar*, Jadhav Santosh, Mane Pandurang,
More informationSpectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form
Spectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form Kailash P Prajapati *, A Bhandari INDO GLOBAL JOURNAL OF PHARMACEUTICAL SCIENCES ISSN 2249-1023 Faculty of Pharmaceutical
More informationResearch Article Available online at
Research Article Available online at www.jgtps.com ISSN: 2230-7346 Volume- 5, Issue -2, pp-1522-1527- April June (2014) DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CITICOLINE
More informationResearch Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC
ISSN 2395-3411 Available online at www.ijpacr.com 248 Research Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC Mantha Vebkatesh* and A. Yasodha
More informationSpectrophotometric Estimation of Paracetamol in Bulk and Pharmaceutical Formulations
ISSN: 0973-4945; CODEN ECJHAO E- http://www.e-journals.net 2011, 8(1), 149-152 Spectrophotometric Estimation of in Bulk and Pharmaceutical Formulations P. NAGENDRA Department of, B E T Academy of Higher
More informationSIMPLE AND SENSITIVE VALIDATED REVERSE PHASE HPLC-UV METHOD FOR THE DETERMINATION OF LYMECYCLINE IN PHARMACEUTICAL DOSAGE FORMS
IJPSR (2012), Vol. 3, Issue 07 (Research Article) Received on 26 March, 2012; received in revised form 25 June, 2012; accepted 28 June, 2012 SIMPLE AND SENSITIVE VALIDATED REVERSE PHASE HPLC-UV METHOD
More informationSpectrophotometric determination of ceterizine hydrochloride with Alizarin Red S
Talanta 50 (1999) 887 892 www.elsevier.com/locate/talanta Spectrophotometric determination of ceterizine hydrochloride with Alizarin Red S K. Basavaiah *, SriLatha, J. Manjunatha Swamy Department of Studies
More informationKeywords: Capecitabine, UV spectrophotometry, ICH guidelines ABSTRACT
Human Journals Research Article January 2015 Vol.:2, Issue:2 All rights are reserved by M. Subba Rao et al. Development and Validation of A Simple and Specific UV Spectrophotometric Method for Capecitabine
More informationResearch Article. Figure 1. Chemical structure of doxofylline. Indonesian J. Pharm. Vol. 24 No. 1 : ISSN-p :
Research Article Indonesian J. Pharm. Vol. 24 No. 1 : 14 21 ISSN-p : 0126-1037 DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL
More informationDevelopment and Validation of Stability Indicating RP-HPLC Method for the Determination of Anagrelide HCl in Pharmaceutical Formulation
ISSN 0976 3333 Available Online at www.ijpba.info International Journal of Pharmaceutical & Biological Archives 2013; 4(2): 342-346 ORIGINAL RESEARCH ARTICLE Development and Validation of Stability Indicating
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com HPLC AND UV-SPECTROPHOTOMETRIC ESTIMATION OF TENELIGLIPTIN
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Chemica Sinica, 2011, 2 (4):1-5 ISSN: 0976-8505 CODEN (USA) CSHIA5 Novel spectrophotometric method for the assay of Pitavastatin calcium in pharmaceutical
More informationDissolution Test 5 was validated using a Zodiac C18 brand of L1 column. The typical retention time for atorvastatin is about min.
Atorvastatin Calcium Tablets Type of Posting Posting Date 25 Jan 2019 Official Date 01 Feb 2019 Expert Committee Chemical Medicines Monographs 2 Reason for Revision Compliance In accordance with the Rules
More informationNew Simple UV Spectrophotometric Method for Determination of Mirtazapine in Bulk and pharmaceutical dosage forms
New Simple UV Spectrophotometric Method for Determination of Mirtazapine in Bulk and pharmaceutical dosage forms Sk. Benajeer Department of pharmaceutical chemistry, benajeershaik@gmail.com K. Venkata
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(1):34-39 ISSN: 0976-8688 CODEN (USA): PSHIBD Advance simultaneous determination of paracetamol, thiocolchicoside and aceclofenac
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
75 CHAPTER 3 DEVELOPMENT AND APPLICATION OF STABILITY-INDICATING HPLC METHOD FOR THE DETERMINATION OF NEVIRAPINE AND ITS IMPURITIES IN COMBINATION DRUG PRODUCT 3.1 INTRODUCTION OF DOSAGE FORM AND LITERATURE
More informationDevelopment and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage form
IJPAR Vol.5 Issue 4 Oct - Dec -2016 Journal Home page: ISSN:2320-2831 Research article Open Access Development and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage
More informationDissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form
Research Journal of Recent Sciences ISSN 2277-252 Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form Abstract Rele Rajan
More informationUV spectrophotometric determination of pimozide in bulk and tablet dosage forms
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Short
More informationMETHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS
CHAPTER 6 METHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS School of Pharmaceutical Sciences, Vels University 106 METHOD DEVELOPMENT AND VALIDATION
More informationResearch Article. Simultaneous spectrophotometric estimation of Paracetamol and Aceclofenac by second order derivative method in combined dosage form
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(6):512-517 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Simultaneous spectrophotometric estimation of Paracetamol
More informationValidated spectrophotometric determination of Fenofibrate in formulation
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2010, 1 (1): 173-178 Validated spectrophotometric determination of Fenofibrate in formulation Krishna R. Gupta*, Sonali S. Askarkar,
More informationInternational Journal of Pharma and Bio Sciences V1(1)2010. HPLC method for analysis of Lercanidipine Hydrochloride in Tablets
G. MUBEEN,, MAMTA PAL, AND M.N. VIMALA* Department of, Al-Ameen College of Pharmacy, Bangalore, India. * Corresponding author vimalamn_325@yahoo.co.in ABSTRACT A reverse phase HPLC method was developed
More informationNovus International Journal of Analytical Innovations 2012, Vol. 1, No. 3
Novus International Journal of Analytical Innovations 2012, Vol. 1, No. 3 ISSN 2278-6953 www.novusscientia.org Accepted on October 22, 2012 RP-HPLC method for simultaneous estimation of Avitriptan and
More informationTEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD
APPENDIX V TEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD Validating Chromatographic Methods. By David M. Bliesner Copyright 2006 John Wiley & Sons, Inc. 159 160 APPENDIX V Title: Effective: Document No:
More informationCHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC. Diclofenac (gift sample from M/s Micro Labs Ltd., Pondicherry)
CHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC A UV spectrophotometric method based on the measurement of absorbance at 276nm in phosphate buffer of p H 7.4 was used in the present study of the
More informationPharmacophore 2014, Vol. 5 (2), USA CODEN: PHARM7 ISSN Pharmacophore. (An International Research Journal)
Pharmacophore 2014, Vol. 5 (2), 252-257 USA CODEN: PHARM7 ISSN 2229-5402 Pharmacophore (An International Research Journal) Available online at http://www.pharmacophorejournal.com/ Original Research Paper
More informationSIMULTANEOUS RP HPLC DETERMINATION OF CAMYLOFIN DIHYDROCHLORIDE AND PARACETAMOL IN PHARMACEUTICAL PREPARATIONS.
Ind. J. Anal. Chem Vol. 7 11. 2008 SIMULTANEOUS RP HPLC DETERMINATION OF CAMYLOFIN DIHYDROCHLORIDE AND PARACETAMOL IN PHARMACEUTICAL PREPARATIONS. Authors for correspondence : R. R. Singh1*, M. V. Rathnam,
More informationPravin Kumar et al. / SGVU Journal of Pharmaceutical Research & Education, 2017, 2(1), Research Article
Research Article SGVU Journal of Pharmaceutical Research & Education Journal homepage: http://www.gyanvihar.org/researchjournals/ UV VISIBLE SPECTROSCOPIC METHOD DEVELOPMENT OF ETODOLAC FROM IT S TABLET
More informationValidated Spectrophotometric Method for the Assay of Diacerein in Bulk and Pharmaceutical Formulations B.M.Gurupadayya 1, B.M.
Validated Spectrophotometric Method for the Assay of Diacerein in Bulk and Pharmaceutical Formulations B.M.Gurupadayya 1, B.M.Bharatheesha 2 1 Department of Pharmaceutical Chemistry, J.S.S. College of
More informationDevelopment of Ultraviolet Spectrophotometric Method for Analysis of Lornoxicam in Solid Dosage Forms
Tropical Journal of Pharmaceutical Research April 2012; 11 (2): 269-273 Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. Research Article
More information