Benzodiazepines and Barbiturates
|
|
- Holly Greer
- 6 years ago
- Views:
Transcription
1 Liquid Chromatography Mass Spectrometry SSI-LCMS-004 Benzodiazepines and Barbiturates LCMS-8030 Summary A rapid, accurate, and reliable LC-MS-MS method was developed for the determination of 21 controlled substances in urine. Background Controlled substances play an important role in the practice of medicine but have the potential to be abused. Urinary testing for these drugs is an important tool in preventing diversion and abuse of controlled substances while ensuring patients with legitimate illness receive the medicines they need. Method Authentic standards for 21 benzodiazepines and barbituates were obtained from a licensed chemical supplier. Dilutions were made for optimization of mass spectrometry parameters. Positive and negative ion electrospray with polarity switching was used in Multiple Reaction Monitoring (MRM) mode for analysis. ptimized parameters are shown in Table 1. Quantifier and qualifier ions (not shown) were used for each compound. A Shimadzu Shimpack XR-DS III column (1.6 um, 2 x 50 mm) was used with a binary gradient of 25% AC and water increasing to 85% AC over 36 seconds. The column was equilibrated for 30 seconds. The flow rate was 0.6 ml/min and the column temperature was 50 o C. The injection volume was 10 ml. The standard autosampler needle rinse was used after each injection to eliminate the possibility of carryover. Calibration curves for standards in solution were prepared between 4.12 ng/ml and 1 mcg/ml. Four selected standards (alprazolam, diazepam, amobarbital, and talbutal) were prepared in a matching matrix (synthetic urine)in the concentration range of 37 ng/ml up to 2 mcg/ml. A weighting factor of 1/X was used, and all calibration points were repeated in triplicate. Low and igh concentration QC samples containing each standard at 100 ng/ml and 1 mcg/ml respectively were prepared. Four test samples were prepared containing various amounts of the selected drugs along with an unspiked fifth test sample containing only synthetic urine (see Table 2). The urine spiked samples and the matrix matched calibration curve were diluted five-fold with mobile phase prior to analysis (dilute and shoot). Each test sample and QC sample was measured five times to determine intra-day repeatability. 1
2 - + Br F Figure 1: Structures of selected drugs of abuse Table 1: MRM parameters Type Event# +/- Compound ame (m/z) Dwell (msec) Q1 Pre Bias(V) CE MRM 1 + Lorazepam 320.7> MRM 2 + itrazepam 282.0> MRM 3 + xazepam 286.7> MRM 4 + Bromazepam 315.6> MRM > MRM 6 + Temazepam 300.6> MRM 7 + Prazepam 324.6> MRM > MRM 9 + Triazolam 342.7> MRM 10 + onazepam 316.0> MRM 11 + Flunitrazepam > MRM 12 - Barbital 183.1> MRM > MRM 14 - Pentobarbital 225.1> MRM 15 - exobarbital 235.1> MRM 16 - Butabarbital 211.1> MRM 17 - Phenobarbital 23> MRM 18 - Methylphenobarbital 245.1> MRM > MRM 20 - Secobarbital 237.1> MRM 21 - Aprobarbital 209.1> Measurement Time 2
3 2,602,753 Lorazepam 663,895 itrazepam 4,577,109 xazepam 1,380,717 Bromazepam 1,200,379 8,024,220 Temazepam 8,466,527 Prazepam 497, ,738 Triazolam 126,778 onazepam 910,480 Flunitrazepam 38,350 Barbital- 279,760 Pentobarbital- 12,535 exobarbital- 86,340 Butabarbital- 191,712 Phenobarbital- 32,368 Methylphenobarbital- 19,903 Secobarbital- 32,894 Aprobarbital min Figure 2: LC-MS-MS Chromatograms of the standard mix in solution 3
4 Lorazepam r 2 = itrazepam 2.0 xazepam r 2 = Bromazepam 2 Conc. 2.5 r 2 = Temazepam 2.5 Prazepam 2 Conc. Triazolam onazepam 2.0 r 2 = Flunitrazepam r 2 = r 2 = Conc. Pentobarbital Area(x1,000) exobarbital r 2 = Butabarbital r 2 = Phenobarbital 2.0 Methylphenobarbital r 2 = Secobarbital r 2 = Aprobarbital r 2 = Figure 3: Calibration curves for standards in solution 4
5 2.1 (x1,000) 1:284.60>193.05(+) (x1,000) 1:284.60>193.05(+) (x1,000) 2:308.70>280(+) (x1,000) 2:308.70>280(+) :223.10>42.00(-) 3:223.10>42.00(-) :225.10>42.00(-) 4:225.10>42.00(-) Figure 4: Blank injection immediately following matrix-matched high standard Figure 5: Matrix Blank Spiked Measured RSD% Spiked Measured RSD% Spiked Measured RSD% Spiked Measured RSD% Lower QC Upper QC Test Sample 1 0 D 0 D Test Sample D 0 D 0 D Test Sample D 0 D Test Sample D D Test Sample 5 0 D 0 D 0 D 0 D (Matrix Blank) Table 2: Quantitative results of high and low QC, four spiked test samples, and the matrix blank. Each sample was run five times and the RSD% is shown. Results and Discussion: All 21 compounds eluted within the 1.25 minute UPLC run time with excellent peak shape and retention time stability. o carryover was detected in the blank injection immediately folowing the upper level matrix-matched calibration curve sample. Calibration curves for all compounds are shown in Figure 3. As shown in Table 2, the accuracy for all matrix-matched QC and test samples was within ±20% and the repeatability was within ±15% RSD. igher precision and accuracy could be easily achieved by using an external or internal standard as appropriate. As expected, no analytes were detected in the unspiked matrix sample. Conclusion: A rapid, accurate, and reproducible UPLC-MS-MS method for drugs of abuse in urine was developed. Dilution was the only required sample treatment prior to analysis. 5
6 LCMS-8040 LCMS-8030 LCMS-8080 LCMS-2020 LCMS-IT-TF Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. Established in 1975, Shimadzu Scientific Instruments (SSI), the American subsidiary of Shimadzu Corporation, provides a comprehensive range of analytical solutions to laboratories throughout orth, Central, and parts of South America. SSI maintains a network of nine regional offices strategically located across the United States, with experienced technical specialists, service and sales engineers situated throughout the country, as well as applications laboratories on both coasts. For information about Shimadzu Scientific Instruments and to contact your local office, please visit our Web site at Shimadzu Corporation SIMADZU SCIETIFIC ISTRUMETS, IC. Applications Laboratory 7102 Riverwood Drive, Columbia, MD Phone: Fax: URL For Research Use nly. ot for use in diagnostic procedures. The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu. The information contained herein is provided to you as is without warranty of any kind including without limitation warranties as to is accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. This publications is based upon the information available to Shimadzu on or before the date of publication, and subject to change without notice. Shimadzu Scientific Instruments, 2012 First Edition: ctober, 2012
Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine using LC-MS/MS
Liquid Chromatography Mass Spectrometry SSI-LCMS-096 Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine using LC-MS/MS LCMS-8045 Summary: A short, robust quantitative method for the analysis
More informationSimultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS
Simultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS TIAFT 204 Toshikazu Minohata, Keiko Kudo 2, Kiyotaka Usui 3, Noriaki Shima 4, Munehiro Katagi 4, Hitoshi
More informationHigh-sensitivity, high-throughput quantitation of catecholamine metabolites in urine by LC/MS/MS for clinical research
PO-CON1655E High-sensitivity, high-throughput quantitation of catecholamine metabolites in urine by LC/MS/MS for clinical research ASMS 2016 MP-079 Atsuhiko TOYAMA 1, Kumie SATOU 2, Yuki NAKAMURA 2, Ichiro
More informationQuantitative Analysis of Opioids Using a Triple-Quadrupole GC/MS/MS
PO-CON1408E Quantitative Analysis of Opioids Using a Pittcon 2014 1090-8P Laura Chambers, Richard Whitney, Ph.D., Nicole M. Lock, Zhuangzhi Max Wang, Ph.D., Clifford M. Taylor; Shimadzu Scientific Instruments,
More informationScreening of pesticides in water using SPE on line
PO-CON E Screening of pesticides in water using SPE on line HPLC 2013 SAMP-38 Stefano Zaza 1, Stefano Maria Lucini 1 1 Shimadzu Corporation, Milano, Italy 1. Introduction Pesticides are widely used in
More informationHighly sensitive quantitative estimation of genotoxic impurities from API and drug formulation using LC/MS/MS
P-CN467E Highly sensitive quantitative estimation of genotoxic impurities from API and drug formulation using LC/MS/MS ASMS 24 TP496 Shruti Raju, Deepti Bhandarkar, Rashi Kochhar, Shailesh Damale, Shailendra
More informationAnalysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS. No. GCMS No. SSI-GCMS-1401
Gas Chromatograph Mass Spectrometer No. GCMS-1401 Analysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS Introduction Development of methods for analysis of drugs of abuse has become a high
More informationDevelopment of high speed CYP cocktail inhibition assay using UHPLC-MS/MS
PO-CON E Development of high speed CYP cocktail inhibition assay using ASMS 213 W2-4 Ichiro Hirano 1, Miho Kawashima 1, Natsuyo Asano 1, Kiyomi Arakawa 1, Yoshihiro Hayakawa 1 1 Shimadzu Corporation. 1,
More informationMulti-residue analysis of pesticides in crude food extracts using a simple extraction technique and LC/MS/MS
PO-CON1529E Multi-residue analysis of pesticides in crude food extracts using a simple extraction technique and LC/MS/MS ASMS 215 WP 77 Miho Kawashima 1, Yuka Fujito 2, Yayoi Ichiki 3, Miho Sakai 4, Takashi
More informationA Method for Simultaneous Analysis of 174 Pesticides in Grape Using GC-MS/MS
PO-CON1518E A Method for Simultaneous Analysis of 174 Pesticides Pittcon 2015 2220-12P Feifei Tian, Xizhi Wang, Shiheng Luo, Guixiang Yang, Taohong Huang, Shin-ichi Kawano, Yuki Hashi, Shimadzu Global
More informationPreparative Separation of Active Components from Natural Products using Low-pressure Gradient Preparative HPLC
PO-CON1405E Preparative Separation of Active Components from Natural Products using Low-pressure Gradient Preparative HPLC Pittcon 014 50-7P Kenichiro Tanaka 1, William Hedgepeth 1, Lincoln Grimes, Tsutomu
More informationAnalysis of Residual Solvents in Pharmaceuticals (USP<467>) with Shimadzu GC-2010 Plus and HS-10 Headspace Sampler
No. SSI-GC- Gas Chromatography No. GC- Analysis of Residual Solvents in Pharmaceuticals (USP) with Shimadzu GC- Plus and HS- Headspace Sampler Introduction Organic solvents are routinely used in manufacturing
More informationEPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS
EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS Christopher Borton AB SCIEX Golden, Colorado verview Described here is the analysis of
More informationDetermination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry
PO-CON1619E Determination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry Pittcon 2016 480-25 Yan Wang, Feifei Tian, Yueqi Li, Guixiang Yang, Taohong
More informationDetermination of VOCs by USEPA Method 8260 with Extended Dynamic Range using Fast, Sensitive Capillary GC/MS
PO-CON1609E Determination of VOCs by USEPA Method 8260 with Extended Dynamic Range using Fast, Sensitive Capillary GC/MS Pittcon 2016 1110-1 Brahm Prakash, William Lipps, Di Wang, Shilpi Chopra, Nicole
More informationisobaric Barbiturates in Serum using LDTD- MS/MS combined with differential mobility
Ultra-Fast Separation Ultra-Fast and Quantification Separation and of isobaric Barbiturates in Serum using LDTD- Quantification of isobaric MS/MS combined with differential mobility spectrometry Barbiturates
More informationEPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS
Application Note EPA Method 535 EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS API 3200 LC/MS/MS System Overview Described here is the
More informationNew Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses
New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses Technical Overview Authors Abstract Peter Stone, Thomas Glauner, Frank Kuhlmann, Tim Schlabach and Ken Miller
More informationForensic and toxicological drug screening using liquid chromatography-tandem mass spectrometry with MS/MS library based identification
POCON696E Forensic and toxicological drug screening using liquid chromatographytandem mass spectrometry with MS/MS library based identification MSACL 6 EU Alan Barnes, Tiphaine Robin, Neil Loftus, Pierre
More informationA novel approach to the analysis of multivitamin by online supercritical fluid extraction/supercritical fluid chromatography
PO-CON1618E A novel approach to the analysis of multivitamin by online supercritical fluid extraction/supercritical fluid chromatography Pittcon 216 83-12 Qiang Li, Hongyuan Hao, Taohong Huang, Shin-ichi
More informationAnalysis of Pesticides in Baby Foods Using a Triple-Quadrupole GC/MS/MS
PO-CONE Analysis of Pesticides in Baby Foods Using a Pittcon 01 -P Laura Chambers, Richard Whitney, Ph.D., Nicole M. Lock, Zhuangzhi Max Wang, Ph.D., Clifford M. Taylor; Shimadzu Scientific Instruments,
More informationRapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry
Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Application Note Food Authors Jianqiu Mi, Zhengxiang Zhang, Zhixu Zhang,
More informationAnalytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS
Application Note Clinical Research Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Authors Yanan Yang 1, Victor Mandragon 2, and Peter Stone 1 1
More informationResearch of Polychlorinated Biphenyls (PCBs) in Vegetables by GC-MS/MS
PO-CONE Research of Polychlorinated Biphenyls (PCBs) Pittcon 0 90-8 Xizhi Wang, Shiheng Luo, Feifei Tian, Jun Fan, Guixiang Yang, Taohong Huang, Shin-ichi Kawano, Yuki Hashi Shimadzu Global COE, Shimadzu
More informationEvaluation of a novel glass vial overcoming adsorption effect for pharmaceutical drugs
PO-CON1577E Evaluation of a novel glass vial overcoming adsorption effect for pharmaceutical drugs HPLC 215 PSB-PHARM-17 Minori Nakashima*(1), Yuki Sato (2,3), Takeshi Kuwahara (3), Azusa Uchida (1), Takenao
More informationDetermination of N-Nitrosamines by USEPA Method 521 using Triple Quadrupole Gas Chromatography Mass Spectrometry
PO-CON111E Determination of N-Nitrosamines by USEPA Method 521 using Triple Quadrupole Gas Chromatography Mass Spectrometry Pittcon 201 1120-2 Brahm Prakash, William Lipps, Di Wang, Shilpi Chopra, Nicole
More informationAnalysis of allergens found in cosmetics using MDGC-GCMS (Multi-Dimensional Gas Chromatograph Mass Spectrometer)
PO-CON9E Analysis of allergens found in cosmetics using MDGC-GCMS (Multi-Dimensional Gas Chromatograph Mass Spectrometer) ASMS 0 TP7 Sanket Chiplunkar, Prashant Hase, Dheeraj Handique, Ankush Bhone, Durvesh
More informationDefinitive EtG/EtS LC-MS/MS Analysis:
Definitive / LC- Analysis: A Rugged 4-Min Method for High-Throughput Labs By Justin Steimling and Frances Carroll Abstract Methods for monitoring alcohol consumption biomarkers and are generally limited
More informationAnalysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research
Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Heather A Brown, 1 Claudia Rossi, 2 and Lisa J Calton 1 1 Waters Corporation, Wilmslow, UK 2
More informationStructural Analysis by In-Depth Impurity Search Using MetID Solution and High Accuracy MS/MS
C146-E118 Structural Analysis by In-Depth Impurity Search Using MetID Solution and High Accuracy MS/MS Technical Report vol.16 1. Introduction MetID Solution is a software application that was developed
More informationHighly sensitive quantitative analysis of Felodipine and Hydrochlorothiazide from plasma using LC/MS/MS
PO-CON18E Highly sensitive quantitative analysis of Felodipine and Hydrochlorothiazide from plasma using LC/MS/MS ASMS 201 TP97 Shailendra Rane, Rashi Kochhar, Deepti Bhandarkar, Shruti Raju, Shailesh
More information+ butanal + 2-picolineborane. + acetic acid. + butanal + 2-picolineborane. + acetic acid
PO-CON1751E Quantitation of plasma metanephrine and normetanephrine by derivatization using an integrated LC-MS/MS analyzer equipped with fully-automated sample preparation device ASMS 2017 ThP-099 Atsuhiko
More informationChiral Separation Using SFC and HPLC
PO-CON6E Chiral Separation Using and HPLC Pittcon 06 760-6 Satoru Watanabe, Hidetoshi Terada, Takato Uchikata, Yohei Arao, Kenichiro Tanaka, Yasuhiro Funada Shimadzu Corporation, Kyoto, Japan; Shimadzu
More informationCOMPREHENSIVE LC X LC ANALYSIS OF FLAVORING INGREDIENTS IN DISTILLED SPIRITS
PO-CON1574E COMPREHENSIVE LC X LC ANALYSIS OF FLAVORING INGREDIENTS IN DISTILLED SPIRITS HPLC 2015 PSB-MULTI-01 Gesa J. Dr. Schad; Brigitte Dr. Bollig; Robert Ludwig; Björn T. Dr. Erxleben Shimadzu Europa
More informationQuantitative Determination of Dioxins in Drinking Water by Isotope Dilution using Triple Quadrupole GC-MS/MS
PO-CON1646E Quantitative Determination of Dioxins in Drinking Water by Isotope Dilution using Triple Quadrupole GC-MS/MS ASMS 2016 ThP 152 Jackie, Chiew Mei Chong, Hui Xian Crystal Yeong, Cynthia Melanie
More informationEvaluation of a New HPLC, a New Tandem MS and a New Data Processing Software for General Clinical Use
Evaluation of a New HPLC, a New Tandem MS and a New Data Processing Software for General Clinical Use Shih-Tse Jason Lai, Jia Wang, Brad Hart, Kristine Van Natta, Marta Kozak, Jorge Valdivia, Haibo Wang,
More informationPlasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS
Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 129 Infi nity LC, and 646 Triple Quadrupole LC/MS Application Note Clinical Research Authors Linda Côté and Christophe
More informationMaximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System
Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Application Note Authors Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon, and
More informationA Study of Stability, Robustness and Time Efficiency of a New HPLC and a New Tandem MS
A Study of Stability, Robustness and Time Efficiency of a New HPLC and a New Tandem MS Jason Lai, Jia Wang, Brad Hart, Pavel Aronov, Kristine Van Natta, Marta Kozak, Jorge Valdivia, Andy Jacobs, Haibo
More informationThe Use of the ACQUITY QDa Detector for a Selective, Sensitive, and Robust Quantitative Method for a Potential Genotoxic Impurity
The Use of the ACQUITY QDa Detector for a Selective, Sensitive, and Robust Quantitative Method for a Potential Genotoxic Impurity Janet Hammond Waters Corporation, Wilmslow, UK APPLICATION BENEFITS High
More informationSimultaneous determination of 74 kinds of pesticides in Chinese herbal medicine listed in Chinese pharmacopoeia (2015) by GPC-GC-MS/MS
PO-CON1555E Simultaneous determination of 74 kinds of pesticides in Chinese herbal medicine listed in Chinese pharmacopoeia (215) ASMS 215 ThP 34 Huiyan Yang, Jun Fan, Taohong Huang, Shin-ichi Kawano,
More informationHigh Sensitivity HPLC Analysis of Perchlorate in Tap Water Using an Agilent 6460 Triple Quadrupole LC/MS System
High Sensitivity HPLC Analysis of Perchlorate in Tap Water Using an Agilent 66 Triple Quadrupole LC/MS System Application Note Environmental Authors Don Noot, Matthew Noestheden, and Ralph Hindle Vogon
More informationQuantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS
Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1
More informationImproved Extraction of THC and its Metabolites from Oral Fluid Using Oasis PRiME HLB Solid Phase Extraction (SPE) and a UPLC CORTECS C 18
Using Oasis PRiME HLB Solid Phase Extraction (SPE) and a UPLC CORTECS C 18 Column Xin Zhang, Jonathan P. Danaceau, and Erin E. Chambers Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Semi-validated
More informationExtraction of Cocaine and Metabolites From Urine Using ISOLUTE SLE+ prior to LC-MS/MS Analysis
Extraction of Cocaine and Metabolites From Urine Using ISOLUTE SLE+ prior to LC-MS/MS Analysis Application Note AN772 Introduction This application note describes the extraction of cocaine and a full range
More informationQuantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS
Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1
More informationQuantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS. Application. Authors. Abstract.
Quantitative Analysis of Water-Soluble B-Vitamins in Cereal Using Rapid Resolution LC/MS/MS Application Food Analysis Authors Sheher Mohsin Agilent Technologies, Inc. Schaumburg, Il USA Michael Zumwalt
More informationAnalysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ8030. No. GCMS No. SSI-GCMS-1404
Gas Chromatograph Mass Spectrometer No. GCMS-1404 Analysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ8030 Introduction Contamination of consumer products with pesticides is a growing concern
More informationAnalysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method
Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method By Sharon Lupo and Frances Carroll Abstract The analysis of synthetic cannabinoids and their metabolites
More informationUltrafast Analysis of Methadone and Metabolite EDDP in Urine by the Agilent RapidFire High-Throughput Mass Spectrometry System
Ultrafast Analysis of and Metabolite in Urine by the Agilent RapidFire High-Throughput Mass Spectrometry System Application Note Forensic Toxicology Authors Mohamed Youssef and Vaughn P. Miller Agilent
More informationApplying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis
PO-CON1768E Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide ASMS 2017 TP-194 David Baker 1, Christopher Titman 1, Neil Loftus 1, Jonathan Horner
More informationMacrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS
Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS Application Note Food Testing and Agriculture Author Chen-Hao (Andy) Zhai and Rong-jie Fu Agilent Technologies (Shanghai)
More informationQuantitative analysis of small molecules in biological samples. Jeevan Prasain, Ph.D. Department of Pharmacology & Toxicology, UAB.
Quantitative analysis of small molecules in biological samples 100 Jeevan Prasain, Ph.D. Department of Pharmacology & Toxicology, UAB % 0 300 500 700 900 1100 1300 1500 1700 m/z Class Overview Introduction
More informationSupporting Information. Sweetened Swimming Pools and Hot Tubs
Supporting Information Sweetened Swimming Pools and Hot Tubs Lindsay K Jmaiff Blackstock, Wei Wang, Sai Vemula, Benjamin T Jaeger and Xing-Fang Li * Division of Analytical and Environmental Toxicology,
More informationPittcon P
PO-CON1701E 76% Increase in Throughput for Determination of Semi-Volatiles Using Narrow-Bore GC Columns and Rapid Data Acquisition with a Highly Sensitive Quadrupole GCMS System Pittcon 2017 1440-14P Brahm
More informationExtraction of Methylmalonic Acid from Serum Using ISOLUTE. SAX Prior to LC-MS/MS Analysis
Application Note AN89.V.1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Page 1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Prior to LC-MS/MS Analysis Sample Preparation
More informationTomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories
APPLICATION NOTE 65200 Tomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories Authors Neloni Wijeratne, Claudia Martins, Mary
More informationJonathan P. Danaceau, Erin E. Chambers, and Kenneth J. Fountain Waters Corporation, Milford, MA USA APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS
Rapid and Simultaneous Analysis of Urinary Catecholamines and Metanephrines Using Mixed-Mode SPE and Hydrophilic Interaction Chromatography (HILIC) for Clinical Research Jonathan P. Danaceau, Erin E. Chambers,
More informationExtraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE. Myco Prior to LC-MS/MS Analysis
Application Note AN785 Extraction of Aflatoxins and chratoxin From Dried Chili Using ISLUTE Myco prior to LC-MS/MS Analysis Page Extraction of Aflatoxins and chratoxin from Dried Chili Using ISLUTE Myco
More informationAssay Transfer from HPLC to UPLC for Higher Analysis Throughput
MAY 2005 SEPARATION SCIENCE REDEFINED 31 Assay Transfer from HPLC to UPLC for Higher Analysis Throughput A typical HPLC assay was transferred and optimized for a Waters ACQUITY UPLC system to achieve both
More informationLC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode
LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode Application Note Authors Siji Joseph Agilent Technologies India Pvt. Ltd.
More informationYun W. Alelyunas, Mark D. Wrona, Russell J. Mortishire-Smith, Nick Tomczyk, and Paul D. Rainville Waters Corporation, Milford, MA, USA INTRODUCTION
Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma Yun W. Alelyunas, Mark
More informationPsychoactive Drugs Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 18 Psychoactive Drugs Urine LC-MS/MS Analysis Kit User Manual ZV-2001-0200-20 200 2-8 C Page 2 / 18 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE...
More informationFast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma
Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Jon Bardsley 1, James Goldberg 2 1 Thermo Fisher Scientific, Runcorn, UK; 2 Thermo Fisher Scientific, West Palm Beach,
More informationLC-MS/MS Method for the Determination of Diclofenac in Human Plasma
LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core
More informationDevelopment of 2D-LC/MS/MS Method for Quantitative Analysis of 1α,25-Dihydroxylvitamin D3 in Human Serum
PO-CON147E Development of 2D-LC/MS/MS Method for Quantitative Analysis of 1α,25-Dihydroxylvitamin D3 in Human Serum ASMS 214 WP449 Daryl Kim Hor Hee 1, Lawrence Soon-U Lee 1, Zhi Wei Edwin Ting 2, Jie
More informationTecan AC Extraction Plate
Tecan AC Extraction Plate Simple sample preparation for the determination of vitamin D in serum by LC-MSMS Introduction A growing number of laboratories have moved from the use of immunoassay methods to
More informationQuantitative Analysis and Identification of Migrants in Food Packaging Using LC-MS/MS
Quantitative Analysis and Identification of Migrants in Food Packaging Using LC-MS/MS Cécile Busset 1 and Stephen J. Lock 2 1 AB SCIEX, Paris (France); 2 AB SCIEX, Warrington, Cheshire (U.K.) Introduction
More informationDetermination of Hormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell HPH Column (EPA 539)
Determination of ormones in Drinking Water by LC/MS/MS Using an Agilent InfinityLab Poroshell P Column (EPA 539) Application Note Environmental Authors Rong-jie Fu and Chen-ao (Andy) Zhai Agilent Technologies
More informationAnalyst Software. Peptide and Protein Quantitation Tutorial
This document is provided to customers who have purchased AB Sciex equipment to use in the operation of such AB Sciex equipment. This document is copyright protected and any reproduction of this document
More informationAgilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes
Agilent 6460 Triple Quadrupole LC/MS System with an Agilent 1290 Infinity LC For Multi-Plant Growth Regulator Analysis in Grapes Application Note Food Safety Author Sunil Kulkarni Agilent Technologies
More informationMetabolomic Profiling of Accurate Mass LC-MS/MS Data to Identify Unexpected Environmental Pollutants
Metabolomic Profiling of Accurate Mass LC- Data to Identify Unexpected Environmental Pollutants André Schreiber 1, David Cox 1, Nadia Pace 1, Christopher Borton 2 1 AB SCIEX, Concord, ntario, Canada; 2
More informationHigh Performance Liquid Chromatography. Table 1: Allowed HPLC Adjustment of USP <621> and EP <2.2.46>
High Performance Liquid Chromatography HPLC-9 Ultra-high Speed Analysis of Ibuprofen within USP Allowed Limits by Nexera Method Scouting In recent years, high-throughput analytical techniques have
More informationImprove Peak Shape and Productivity in HPLC Analysis of Pharmaceutical Compounds with Eclipse Plus C8 Columns Application
Improve Peak Shape and Productivity in HPLC Analysis of Pharmaceutical Compounds with Eclipse Plus C8 Columns Application Pharmaceuticals Authors John W. Henderson Jr., Nona Martone, and Cliff Woodward
More informationVMA, HVA, 5-HIAA Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 11 VMA, HVA, 5-HIAA Urine LC-MS/MS Analysis Kit User Manual ZV-3030-0200-20 200 2-8 C Page 2 / 11 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE... 3
More informationAssay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer
38 Current Trends in Mass Spectrometry November 6 Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer This article demonstrates the improved
More informationUsing UHPLC-Triple Quadrupole MS/MS to Detect the Presence of Bark Extract and Yohimbine Adulteration in Dietary Supplements and Botanicals
Using UHPLC-Triple Quadrupole MS/MS to Detect the Presence of Bark Extract and Adulteration in Dietary Supplements and Botanicals Application Note Food Testing & Agriculture Authors James Neal-Kababick
More informationUltrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System
Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System Application Note Authors Mohamed Youssef and Vaughn P. Miller Agilent
More informationQuantitative Analysis of β-lactam Antibiotics in Human Plasma by High Sensitivity LC/MS/MS Method
PO-CON1738E Quantitative Analysis of β-lactam Antibiotics in Human Plasma by High Sensitivity LC/MS/MS Method ASMS 2017 ThP 079 Daryl Kim Hor Hee 1 ; Zhi Wei Edwin Ting 2 ; Lawrence Soon-U Lee 1 ; Jie
More informationAppNote 1/2013. Determination of Barbiturates and 11-Nor-9-carboxy- 9 -THC in Urine using Automated Disposable Pipette Extraction (DPX) and LC/MS/MS
AppNote 1/2013 Determination of Barbiturates and 11-Nor-9-carboxy- 9 -THC in Urine using Automated Disposable Pipette Extraction (DPX) and LC/MS/MS Fred D. Foster, Oscar G. Cabrices, John R. Stuff, Edward
More informationQuantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 1260 Infinity Binary LC system with UV detection
Quantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 126 Infinity Binary LC system with UV detection Application Note Food Author Srividya Kailasam Agilent Technologies,
More informationThe Quantitation and Identification of Coccidiostats in Food by LC-MS/MS using the AB SCIEX 4000 Q TRAP System
The Quantitation and Identification of Coccidiostats in Food by LC-MS/MS using the AB SCIEX 4000 Q TRAP System Bertram ieland 1 and Stephen Lock 2 1 AB SCIEX ieuwerkerk aan den Ijssel, The etherlands;
More informationHigh-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes
High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes Joe DiBussolo, Marta Kozak Thermo Fisher Scientific, San Jose, CA Application
More informationDetermination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry
Determination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry Application Note Doping Control Authors Prof. Maria Kristina Parr Freie Universität Berlin Institute
More informationAnion Analysis of Ophthalmic Irrigation Solutions with the Shimadzu Prominence IC System Craig Young, PhD, Sr. Tech Support Specialist
High Performance Liquid Chromatography SSI HPLC 5 Anion Analysis of Ophthalmic Irrigation Solutions with the Shimadzu Prominence IC System Craig Young, PhD, Sr. Tech Support Specialist Introduction Ophthalmic
More informationApplication Note LCMS-110 Development of a Targeted Quantitative LC-MS/MS Method for 431 Positive and Negative Ion Pesticides in a Single Analysis
Application Note LCMS-110 Development of a Targeted Quantitative LC-MS/MS Method for 431 Positive and Negative Ion Pesticides in a Single Analysis Abstract A rapid, targeted quantitative UHPLC-triple quadrupole
More informationDetermination of Organochlorine Pesticides and Polychlorinated Biphenyls Using GC/MS/MS Operated in the MRM Mode
PO-CON1610E Determination of Organochlorine Pesticides and Polychlorinated Biphenyls Using GC/MS/MS Operated in the MRM Mode Pittcon 2016 1110-2 Brahm Prakash, William Lipps, Di Wang, Shilpi Chopra, Nicole
More informationImproved Automated Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS
Improved Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS Abstract In this work, we demonstrate an improved automated sample preparation for liquid chromatography-tandem mass spectrometry
More informationFractionation of Acidic, Basic, and Neutral Drugs from Plasma with an SPE Mixed Mode Strong Cation Exchange Polymeric Resin (Agilent SampliQ SCX)
Fractionation of Acidic, Basic, and Neutral Drugs from Plasma with an SPE Mixed Mode Strong Cation Exchange Polymeric Resin (Agilent SampliQ SCX) Application Note Forensic Toxicology Authors Bellah. Pule,
More informationFast and Accurate Analysis of Fluorotelomer Alcohols and Acrylates using Triple Quadrupole GC-MS/MS
POCON1647E Fast and Accurate Analysis of Fluorotelomer Alcohols and Acrylates using Triple Quadrupole GCMS/MS ASMS 2016 ThP 6 Cynthia Melanie Lahey 1, Guo Wei Elgin Ting 2, Hui Xian Crystal Yeong 1, Jackie
More informationAB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching Scores by Removal of Isobaric Interferences
AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching s by Removal of Isobaric Interferences Differential Mobility Used as a Tool to Address Selectivity Challenges Adrian M. Taylor
More informationImproved Screening for 250 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer
Improved Screening for 2 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer Mary lackburn, Jia Wang, Jonathan eck, Charles Yang, Dipankar Ghosh, Thermo Fisher Scientific, San Jose, C,
More informationLC800. Smart HPLC. Until now UHPLC From now Smart HPLC
LC800 Smart HPLC Until now UHPLC From now Smart HPLC Smart HPLC Leads to Ultimate Performance Patent Pending S LC800 is a completely new and unique concept, designed for maximum performance in high resolution
More informationHighly sensitive and rapid analysis of synthetic dyes in sea food by LC/MS/MS
PO-CON1745E Highly sensitive and rapid analysis of synthetic dyes in sea food by LC/MS/MS ASMS 2017 MP 189 Shailendra Rane 1, Ashutosh Shelar 1, Shailesh Damale 1, Rashi Kochhar 1, Purshottam Sutar 1,
More informationOverview. Introduction. Method Details. André Schreiber and Yun Yun Zou AB SCIEX, Concord, Ontario, Canada
Comprehensive Quantitation and Identification of Pesticides in Food Samples using LC-MS/MS with Scheduled MRM, Fast Polarity Switching, and MS/MS Library Searching André Schreiber and Yun Yun Zou AB SCIEX,
More informationFast Screening and Quantitation of Perfluorinated Sources from Textiles using Chemical Ionization GC-MS
PO-CON1731E Fast Screening and Quantitation of Perfluorinated Sources from Textiles using Chemical Ionization GC-MS ASMS 2017 TP-295 Hui Xian Crystal Yeong 1, Stephany Olivia 2, Lai Chin Loo 1 1 Application
More informationDetermination of Chlorinated Acid Herbicides in Soil by LC/MS/MS Application Note
Determination of Chlorinated Acid Herbicides in Soil by LC/MS/MS Application Note Environmental Author Chin-Kai Meng Agilent Technologies 85 Centerville Road Wilmington, DE 988-6 USA Abstract Chlorinated
More informationAuthors. Introduction. Abstract. Forensics
Determination of Cocaine, Benzoylecgonine, Cocaethylene, and Norcocaine in Human Hair Using Solid-Phase Extraction and Liquid Chromatography with Tandem Mass Spectrometric Detection Application Forensics
More informationSeparation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers
Application Note Forensic and Toxicology - Criminalistics Separation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers Using the Agilent 126 Infinity II SFC and Detection by Coupled
More informationVOC Analysis of Water-Based Coatings by Headspace-Gas Chromatography
VOC Analysis of Water-Based Coatings by Headspace-Gas Chromatography Introduction ASTM International publishes a number of different methods for the determination of volatile compounds in water-based coatings.
More information