Analyzing Hydrogen Peroxide Solution Chemistry 30
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1 NAME: Partner: Date: In this investigation, you assume the role of a laboratory technician working in a consumer advocacy laboratory. Your task is to test the concentration claim of a consumer hydrogen peroxide solution. The labelled percent concentration of hydrogen peroxide on the commercial product is used as the Prediction. Pre-Lab Questions 1. In this lab you will be using a diluted solution of hydrogen peroxide. What does it mean to dilute a solution? How would you dilute something by a factor of 5? How would a dilution factor of 10 be different? 2. What is the molar concentration of a 5% W/V H2O2(aq) solution? Show your work. Page 1
2 3. Re-write in your own words the titration procedure found on pg 804 in your text book. Include any necessary diagrams. Page 2
3 Purpose The technological purpose of this investigation is to test and evaluate the percent concentration of the consumer solution of hydrogen peroxide. Problem What is the percent concentration of hydrogen peroxide in a consumer product? Prediction Design An acidic solution of the primary standard, iron(ii) ammonium sulfate water (1/6), is prepared and the potassium permanganate solution is standardized by a titration with this primary standard. A 25.0 ml sample of a consumer solution of hydrogen peroxide is diluted to 1.00 L with water (that is, it is diluted by a factor of 40). The standardized potassium permanganate solution is used to titrate the diluted and acidified hydrogen peroxide. The amount concentration of the original hydrogen peroxide is obtained by analysis of the titration evidence, and by using a graph, prepared with a graphing calculator or computer spreadsheet program, of the information in Table 1. Page 3
4 Materials lab apron eye protection Iron(II) Ammonium Sulfate FeSO 4 (NH 4 ) 2 SO 4 6H 2 O(s) Potassium Permanganate KMnO 4 (aq) diluted hydrogen peroxide H 2 O 2 (aq) 2 mol/l sulfuric acid H 2 SO 4 (aq) 100mL beaker 50 ml graduated cylinder 100mL volumetric flask Funnel Electronic balance Distilled H 2 O Sulfuric acid is corrosive. Iron(II) ammonium sulfate, hydrogen peroxide, and potassium permanganate are irritants. Avoid inhaling any solid, and avoid skin or eye contact. Procedure Part 1 Standardization of KMnO4 (aq) Demo 1. Calculate the required mass of FeSO 4 (NH 4 ) 2 SO 4 6H 2 O(s)to prepare ml of a mol/l solution. 2. Measure the required mass of the iron(ii) compound in a clean, dry 100 ml beaker. 3. Dissolve the solid in about 40 ml of H2SO4(aq). 4. Using a funnel, transfer this solution into a clean 100 ml volumetric flask. UJsing distilled water, rinse the beaker twice and fill to volume to complete the preparation of the standard solution. 5. Transfer ml of the standard iron(ii) solution by pipette into clean 250 ml Erlenmeyer flask. 6. Obtain approximately 50mL of KMnO4(aq) in a 100mL beaker 7. Drain distilled water from burette, then rinse twice with distilled letting it drain into waster beaker. Using a funnel, rinse burette with titrant (KMnO4(aq)) letting it drain out. Then fill the burette with KMnO4(aq). Be sure open the stopcock and allow solution to fill the tip of the burette. 8. Titrate the acidic iron(ii) sample with KMnO4(aq). 9. Repeat steps 5 and 6 until three consistent volumes (within 0.1 ml) are obtained. Page 4
5 Part 2 Titration of Hydrogen Peroxide solution (Student completed) 10. Obtain approximately 50mL of dilute hydrogen peroxide in a 100mL beaker. 11. Transfer ml of the diluted hydrogen peroxide solution by pipette into a clean 250 ml Erlenmeyer flask. 12. Using a 10 ml graduated cylinder, add 5 ml of H2SO4(aq) to the hydrogen peroxide solution. 13. Using a funnel, fill the burette with KMnO4(aq). Be sure open the stopcock and allow solution to fill the tip of the burette. 14. Titrate the acidic hydrogen peroxide solution with KMnO4(aq). 15. Repeat steps 10 to 13 until three consistent volumes (within 0.1 ml) are obtained. Clean-Up Procedures 1. Dispose of all solutions into a labelled waste container. 2. Rinse burette with distilled water into a waste beaker until all KMnO4(aq) is removed and water runs clear. Fill burette with distilled water and return to the rack on the wall. 3. Rinse pipette with distilled water into waste beaker and return to pipette holder. 4. Wash all other glassware and equipment, dry and return to cupboards. 5. Wipe down lab bench Page 5
6 Evidence mass of FeSO4 (NH4)2SO4 6H2O (s) to prepare ml of solution = Table 1 Titration of ml of Acidic Iron(II) Standard with KMnO4(aq) Trial final burette initial burette volume of endpoint colour Table 2 Titration of ml of Acidified, Diluted H2O2(aq) with KMnO4(aq) Trial final burette initial burette volume of endpoint colour Analysis 1. Calculate the amount concentration of potassium permanganate solution. 2. Using the answer from #1 calculate the amount concentration of diluted hydrogen peroxide solution. Page 6
7 3. Calculate the amount concentration of original hydrogen peroxide solution. 4. Use the amount concentration to determine percent concentration of original hydrogen peroxide solution: a. Graph the information found in table 1. b. Use line of best fit to determine % concentration. Page 7
8 Evaluation 1) Evaluate the percent concentration of the consumer solution of hydrogen peroxide. a. Calculate the percent error of the tested solution and the manufacturers concentration. Percent error = ( experimental theoretical ) 100 theoretical b. Comment on the validity of the companies claim of the percent concentration of the hydrogen peroxide solution. Page 8
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