Chapter-V. Spectrophotometric and HPLC Methods for the determination of AMIKACIN
|
|
- Nickolas Morton
- 6 years ago
- Views:
Transcription
1 Spectrophotometric and HPLC Methods for the determination of AMIKACIN
2 Amikacin is chemically known as 2(S)-4-amino-N-[(2S,3S,4R,5S)-5- amino-2-[(2s,3r,4s,5s,6r)-4-amino-3,5-dihydroxy-6-(hydroxymethyl) oxan-2-yl]oxy-4-[(2r,3r,4s,5r,6r)-6-(aminomethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-3-hydroxy-cyclohexyl]-2-hydroxy-butanamide Amikacin is an aminoglycoside antibiotic used to treat different types of bacterial infections. Amikacin works by binding to the bacterial ribosomal subunit, causing misreading of mrna and leaving the bacterium unable to synthesize proteins vital to its growth. Amikacin is most often used for treating severe, hospital-acquired infections with multidrug resistant gram negative bacteria such as Pseudomonas aeruginosa, Acinetobacter, and Enterobacter. Amikacin may be combined with a beta-lactam antibiotic for empiric therapy for people with neutropenia and fever. Side effects of amikacin are similar to other aminoglycosides. Kidney damage and hearing loss are the most important side effects. 5.1
3 DRUG PFOFILE Fig Structure of amikacin Systematic (IUPAC) Name 2(S)-4-amino-N-[(2S,3S,4R,5S)-5-amino-2-[(2S,3R,4S,5S,6R)-4-amino-3,5- dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4-[(2r,3r,4s,5r,6r)-6- (aminomethyl)-3,4,5-trihydroxy-oxan-2-yl]oxy-3-hydroxy-cyclohexyl]-2- hydroxy-butanamide. Formula C 22 H 43 N 5 O 13 Mol. mass g/mol 5.2
4 Table-I.5.1 : List of important brand names of amikacin formulations. Brand Formulation Strength Manufacturer Name AMISTAR Vial 100mg Cadila Pharmaceuticals Limited, Mahemdabad Road,Ahmedabad. AMTOP Vial 100mg Ind-Swift Ltd , ChambersW.E Highway, Service Road Mumbai. AMIKEF Vial 100mg Lupin laboratory Bandra Kurla complex, Mumbai. Very few spectrophotometric methods for the determination of amikacin have been reported ( Chapter-I). Literature survey revealed that few analytical methods were reported for the determination of amikacin In this chapter catechol-naio 4 and p-amino acetophenone ( AAP-NaIO 4 ) have been used as coupling agents for the determination of amikacin. A detailed review of literature for catechol ( or AAP) and sodium meta per iodate was reported in chapter-i. 5.3
5 SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF AMIKACIN USING CATECHOL AND SODIUM METAPERIODATE Experimental Results and Discussion 5.4
6 EXPERIMENTAL Preparation of Solutions Catechol: 0.1% solution was prepared by dissolving 0.1 g of catechol sample (A.R.grade: SDFCL Mumbai) in 100 ml of distilled water. Oxidising Agent. Sodium meta periodata, NaIO 4 : g of NaIO 4 (A.R grade: Hi Media laboratories Mumbai-66) was dissolved in distilled water and the total volume was brought to 1 Lt (0.01M). Standard solution of Amikacin. Standard solution of amikacin was prepared by dissolving 100 mg of drug sample [ ALFAKIM-Ranbaxy ] in 100mL of distilled water. Working solutions of drug sample (100 g / ml) were prepared by diluting aliquots of the stock solutions with distilled water. Instrumentation Spectral measurements and absorbance readings were made on Elico SL 177 double beam Spectrophotometer. ph measurements were carried out using Elico ph meter model LI 615. Absorbance curves In order to ascertain the optimum wave lengths ( λ max ) of the colored species formed on mixing amikacin with suitable reagents in appropriate ph medium exhibiting maximum absorbance, the absorption spectra were scanned on a spectrophotometer in the range nm against the 5.5
7 reagent blank using the proposed procedure under experimental conditions (Table II.5.1) and the results are graphically presented in Fig A b s o r b a n c e Concentration µg Fig Standard Curve of Amikacin Establishment of Optimum Conditions Concentration of Reagents: The optimum conditions were established in each case basing on the development of maximum color and its stability and results are presented in Table II.5.1. Among the various oxidizing agents tried, IO 4 - is the best one, followed by H 2 O 2. The other oxidizing agents such as IO 3 -, Fe(III), MnO 4 -, clo -, Fe(CN) 6 3-, are inferior. The efficiency of the oxidizing agent 5.6
8 depends upon its relative reactive tendency towards reactants, (drug, catechol) products (indo-dyes) and also on the behavior of its reduced form. The formation of colored species of same λ max in the case of amikacin with each pair of reagents, (Catechol IO 4 - and AAP-IO 4 - ) suggests that the indo dye formed with both compounds is the same. - However for operational feasibilities only catechol-io 4 related results are - presented although experiments were conducted with AAP-IO 4 reagent also. Order of addition of reagents. The author has carried out series of experiments to test whether variation in the order of addition of reactants ( amikacin, oxidizing agent and catechol) effect the absorbance. The suitable order of addition of reactants for getting maximum absorbance and stability has been found to be, amikacin, catechol and oxidizing agent. The order of addition of reactants influences color development. Any delay in adding catechol to oxidant causes considerable decrease in absorbance depending upon the nature of oxidant. These studies reveal that the oxidant is capable of oxidizing catechol under chosen experimental conditions. 5.7
9 Effect of temperature : All experiments and absorbance measurements were carried out at laboratory temperature ( C). At low temperature (< 20 0 c) the stability of the colored species is less. Effect of solvent: A mixture of, requisite concentrations of amikacin, catechol and oxidizing agent were placed in a separating funnel and was diluted to 25mL with distilled water. After keeping it for some time, for allowing the reaction to complete, 10mL of chloroform or n-butanol (if insoluble in chloroform) was added to the separating funnel and the contents were shaken well for 2 min. and left 10 min to get clear separation of two phases. It was noticed that the colored species formed in the case of amikacin with the reagent (Catechol- IO - 4 ) is extractable in butanol but not into chloroform. The absorbance of the organic phase was measured at appropriate wave length against reagent blank. As solvent extraction did not give any additional advantage, it was excluded in further investigations. The studies on the influence of other water miscible (polar) solvents such as acetonitrile, methanol, t-butyl alcohol, or acetone instead of water revealed that the aqueous medium was the best one for maximum color development. 5.8
10 Effect of Buffer: Aqueous medium without usage of any buffer (or Potassium acid phthalate buffer, ) was found suitable in the determination of amikacin with catechol-per iodate. Stability of Color: The influence of time for maximum color development and stability of the colored species formed between amikacin, catechol, per iodate was found to be 5 min. and the results are incorporated in Table-II.5.1 Table-II.5.1 Experimental conditions Optimum conditions Catechol NaIO 4 Time for max. Stability of Color Color in min in min λ max 1.0 ml 1.0 ml nm Optical Characteristics. Adherence to Beer s Law: In order to test whether the amikacin-catechol-per iodate system adheres to Beer s, the absorbance at λ max of a set of solutions containing varying amounts of amikacin, specified concentrations of catechol and sodium meta per iodate (Table-II.5.1) were measured against reagent blank on a spectrophotometer. The linearity of the plot between absorbance and the concentration range specified in Table-II.5.1 shows that the color 5.9
11 ABSORBANCE system obeys Beer s law, Fig Beer s law limits, molar absorptivity, optimum photometric range, and Sandell s Sensitivity values were calculated and the results are incorporated in Table-III CONCENTRATION µg Fig : Beer s Law Plot for Amikacin Table III.5.1 : Optical Characteristics Reagent Catechol- IO 4 - reagent Amikacin Beer s Law Range µg/25 ml Molar Absorptivity Lt/mol/cm Sandell s Sensitivity µg/cm 2 /0.001 absorbance units Optimum Photometric Range µg/25 ml X
12 In view of all the observations it is felt that the following procedure for the spectrophotometric assay of amikacin using catechol, (or AAP) and oxidizing agent will be highly suitable for routine analysis. Assay procedure: - For Amikacin-using Catechol-IO 4 : Aliquots ranging from 1-4 ml of the working standard solution of Amikacin along with 1 ml of catechol solution and 1 ml of sodium per iodate solution were added to a series of 10 ml graduated test tubes and the tubes were kept aside at room temperature for 5 min. Appropriate quantities of distilled water was added to each tube to make the volume. The absorbance of red colored complex formed was measured at 460 nm against reagent blank, prepared in a similar manner. The amount of amikacin was read from calibration curve prepared with its standard solution under identical conditions. Precision and accuracy: The precision and accuracy of the methods in the determination of amikacin was tested by measuring the absorbance of six replicates each containing a final concentration value, approximately ¾ of Beer s range. The % relative standard deviations and confidence limits (0.05 and 0.01 levels) are presented in Table-IV
13 Table.IV.5.1.Precesion and Accuracy. Amikacin Amount of Drug * % Taken Found Error mg mg Catechol- IO 4 - reagent Amikacin % R.S.D % Range of Error 95% Confidence Limit 99% Confidence Limit ±1.25 ± ±1.32 ±1.72 *Average of six replicates The accuracy of the methods was determined by taking known different amounts (within Beer s law range) of amikacin and estimating these amounts with the proposed methods. The results are incorporated in Table- IV.5.1. The accuracy of the method was further tested in injections with proposed and reported methods. The results of these estimations are incorporated in Table-V.5.1 Table-V.5.1 Analysis of Formulations-Recovery Experiments. Sample Amikacn injection Amikacn injection Labeled Amount mg Mean of % amount found Reported Proposed method method % Recovery Experiments Amount %Recovey added
14 RESULTS AND DISCUSSION As mentioned on page 5.7, AAP-IO - 4 is behaving in a similar manner like - catechol-io 4 in case of λ max, of course with a negligible improvement in color intensity. However the mechanism of color formation is different in - case of catechol-io 4 and AAP-IO - 4 reagent systems. Based on the results furnished in Tables II.5.1 V.5.1 reveal that the method proposed for the spectrophotometric determination of amikacin is simple, rapid, sensitive and specific with reasonable precision and accuracy. The proposed method appears to be superior to many of the reported methods and so it can be employed in routine determinations. Catechol undergoes oxidation in the presence of mild oxidizing agent per iodate to form o-benzo quinone. This o-benzo quinone undergoes coupling with the amino group of amikacin to form red colored chromogen. The oxidative coupling reaction can be represented as follows. 5.13
15 HO O OH NaIO 4 O Amikacin Catechol o-benzo quinone HO NH 2 OH O NH O O OH OH OH O OH N H 2 N O OH O H 2 N OH Red colored indo-dye As AAP contains electron withdrawing group,- CO-CH 3 in para - position to aromatic amine, AAP-IO 4 can successfully be used for the estimation of amikacin. The failure of resorcinol (or pyrogellol) to develop color with all the proposed pairs of reagents may be due to the less reactive nature of its oxidative product, m-benzo quinone, and so it does not undergo coupling reaction giving indo dye. 5.14
16 Conclusion. Hence the author concludes that the proposed spectrophotometric method is sensitive and reproducible for the analysis of Amikacin in pharmaceutical dosage forms with short analysis time. 5.15
17 DEVELOPMENT AND VALIDATION OF AMIKACIN BY RP-HPLC METHOD Experimental Results and Discussion 5.16
18 EXPERIMENTAL Materials and Methods. Instrumentation. The author attempted to develop a liquid chromatographic method for the quantitative estimation of Amikacin. A Scimadzu HPLC equipped with a Luna C 18 column (250 nm X 4.6nm,5µ) an LC 20 AD pump and a SPD 20 AD UV- Visible detector was employed in this study. Chromatographic analysis and data acquision was monitored by using Spinchrome software. A 20 µl Hamilton syringe was used for sample injection. Degassing of the mobile phase was done by using a spectra lab.dga 20A3 Ultra sonic bath sonicator. A Shimadzu electronic balance was used for weighing the materials. The reference samples of Amikacin was supplied by Venus Remedies Limited, India and the branded formulations of Amikacin (AMISTAR and AMLTOP) were used. Chemicals and Solvents. Methanol HPLC grade (Merck Ltd, Worli,Mumbai), Acetonitrile-HPLC grade(merck Ltd,Worli,Mumbai) ortho-phosphoric acid HPLC grade (SD Fine Chemicals, Chennai) were used. 5.17
19 Preparation of mobile phase and stock solutions. A mobile phase is a mixture of acetonitrile, methyl alcohol, and o- phosphoric acid, OPA, (0.1%) in 50:35:15 (v/v) which was prepared by mixing 500 ml of acetonitrile, 350 ml of methyl alcohol, and 150 ml of 0.1% o-phosphoric acid in one Liter flask. This mixture was used as a diluent for preparing working standard solutions of the drug. About 100mg of Amikacin was weighed accurately and transferred into a 100 ml volumetric flask containing 20 ml of mobile phase. The solution was sonicated for 20 min. and then the volume was made up with a further quantity of mobile phase to get 1 mg/ml solution. This solution was suitably diluted with mobile phase to get a working standard solution of 100µg/mL of Amikacin. Optimization of Chromatographic Conditions. Method Development: A systematic study was followed for developing the method for optimization of chromatographic conditions. This was carried out by varying one parameter keeping the other conditions constant at an instant of time. Column: A non polar C 18, 250 nm x 4.6 nm column was chosen as the stationary phase for this study. 5.18
20 Mobile Phase: In order to get sharp peak and base line separation of the components, the author has carried out a number of experiments by varying the commonly used solvents with different compositions and its flow rates. In order to establish ideal separation of the drug under isocratic conditions mixtures of commonly used solvents like water, methanol, acetonitrile, o-phosphoric acid with or without different buffers, in different combinations were tested as mobile phases on a C 18 stationary phase. A mixture of acetonitrile, methyl alcohol, and o-phosphoric acid, OPA, (0.1%) in 50:35:15 (v/v) was proved to be the most suitable of all the combinations since the chromatographic peaks obtained were well defined, resolved and free from tailing. A flow rate of 1.0 ml/min mobile phase was found to be suitable in the studied range of ml/min. Wave length: The spectra of diluted solutions of Amikacin in methanol were record on UV spectrophotometer. The peaks of maximum absorbance wavelengths were observed. The spectra of Amikacin showed a balanced wavelength at 272 nm. Retention Time: Under the above optimized conditions a retention of 9.6 min was obtained for Amikacin. A typical model chromatogram showing the separation of Amikacin is presented in Fig
21 After a thorough study of the various parameters the following optimized conditions mentioned in Table-I.5.2 were followed for the determination of Amikacin in bulk samples and pharmaceutical formulations. Fig Chromatogram of Amikacin 5.20
22 HPLC Report A.P.I- AMIKACIN CONCENTRATION-- 25µg/ml RETENTION TIME (R.T) min AREA THEORETICAL PLATES WAVE LENGTH--272nm MOBILE PHASE-MeOH 35%:ACN,50%: (0.1%)OPA,15% COLUMN--C18 FLOW RATE-1.0 ml/min RUN TIME--12 min PH LINEARITY RANGE µg/ml Table HPLC Report of amikacin 5.21
23 Linearity and Construction of Calibration Curve The quantitative determination of the drug was accomplished by the external standard method. The mobile phase was filtered through a 0.45µ membrane filter before use. The flow rate of the mobile phase was adjusted to 1.0 ml/min. The column was equilibrated with mobile phase for at least 30 min. prior to injection of the drug solution. The column temperature was maintained at 25±1 0 C throughout the study. Linearity of the peak area response was determined by taking six replicates at seven concentration points. Working solutions of Amikacin (range 100µg/ml) were prepared by diluting 10ml volumetric flasks with mobile phase. 20 microliters of the dilution was injected six times into the column. The drug in the eluents was monitored at 272 nm and corresponding chromatograms were obtained. The mean peak areas were noted from the chromatograms and a plot of concentrations over the peak areas was constructed. The regression of the plot was computed by least square method. The linearity was found to be in the range of 1 5 µg/ml between the concentration of Amikacin and peak area response. This regression equation was later used to estimate the amount of Amikacin in pharmaceutical dosage forms. The linearity was shown in Fig and the linearity data and statistical parameters for linearity plot are reported in Tables II.5.2 and III
24 Fig Linearity Graph of amikacin Table II.5.2: Linearity of Amikacin by the proposed HPLC method. CONCENTRATION µg/ml AREA Table III.5.2:Regression Characteristics of the linearity plot of Amikacin PARAMETER VALUE Linearity Range(µg/ml) 1-5 µg/ml Slope(a) Intercept(b) Correlation Coefficient Regression Equation Y= x
25 VALIDATION OF THE PROPOSED METHOD The method was validated in compliance with guidelines of International Conference on Harmonization (ICH). The following parameters were determined for validation. Specificity: The specificity of the method was assessed by comparing the chromatograms obtained from the drug with the most commonly used excipients mixture with those obtained from the blank solution. The blank solution was prepared by mixing the excipients in the mobile phase without the drug. The drug to excipient ratio used was similar to that in the commercial formulations. The commonly used excipients in formulations like lactose, microcrystalline cellulose, ethyl cellulose, hydroxyl propyl methyl cellulose, magnesium stearate and colloidal silicon di oxide were used for the study. The mixtures were filtered through 0.45µ membrane filter before injection. An observation of chromatograms indicates absence of excipients peaks near the drug peak in the study runtime. This indicates that the method is specific. Precision: Precision is the degree of repeatability of an analytical method under normal operational conditions. The precision of the method was studied in terms of repeatability in intra-day assay and inter-day assay (intermediate precision). Method repeatability was studied by repeating the assay three 5.24
26 times in the same day for intra-day precision, and intermediate precision was studied by repeating the assay on three different days, three times each day(inter day precision). The intra day and inter day variation for determination of Amikacin was carried out at four different concentrations. %RSD values are presented in the Table-IV.5.2 shows that the method provides acceptable (<2) intra day and inter day variation. Table-IV.5.2 Intra and Inter-Day Precision Concentration of Amikacin µg/ml Intra-Day Precision Mean amount found % amount found %RSD Inter-Day Precision Mean amount found % amount found %RSD Accuracy: Accuracy of the method is evaluated by standard addition method. An amount of the pure drug at three different concentrations in its solution has been added to the pre analyzed working standard solution of the drug. The sample solutions were analyzed in triplicate at each level as per the proposed method. The percent individual recovery and %RSD for recovery at each level are calculated. The results are tabulated (Table-V.5.2). A 5.25
27 mean recovery ranged from has been obtained by the method indicates its accuracy. Amount taken mg Table-V.5.2 Accuracy Data Amount found Mean Recovery %Recovery Robustness: A study was conducted to determine the effect of deliberate variations in the optimized chromatographic condition of the mobile phase, flow rate, and the ph of the mobile phase. The effect of these changes on the system suitability parameters like tailing factors, the number of theoretical plates, and on assay was studied. A single condition was carried at a time keeping all other parameters constant. The results were found to be within the allowed limits indicate that the method is robust. Variation in composition of mobile phase: The effect of variation in percent organic content in mobile phase was evaluated by changing the 5.26
28 composition of organic component in the mobile phase. The tailing factor and the number of theoretical plates showed a little change with change in mobile phase composition. The values are presented in Table-VI.5.2. Variations in flow rates A study was conducted to determine the effect of variation in flow rate. The system suitability parameters were evaluated at 0.9 ml/min and 1.1 ml/min. The results were within the acceptance criteria. Hence the allowable variation in flow rate is 1.0 ml/min. Variation of Mobile Phase MeOH ACN OPA Table - VI.5.2 Results of Robustness Study Chromatographic Parameters Tailing factor Theoretical plates %Assay Stability of the analytical solution: A study to establish bench to top stability of the drug solution was performed. A freshly prepared working standard solution (25µg/mL of the drug) was analyzed immediately at different time intervals. The tailing factor theoretical plates, and the difference in percent assay at different time intervals were calculated and the results are given in Table-VII.5.2. A maximum difference of 0.361% in the assay at the end of 24 hours was observed. The difference in percent assay meets the acceptance standard. The above study concludes that the standard drug solution is stable for twenty four hours on bench top. 5.27
29 Table VII.5.2 Stability of Standard solution. Time in hours %Assay AMIKACIN % Difference Initial Limit of Detection and Limit of Quantification. Limit of detection (LOD) is defined as the lowest concentration analyte that gives a measurable response. LOD is determined based on signal to noise ratio (S/N) of three times typically for HPLC methods. LOD = 3.3X S.D of y intercept Slope of Calibration curve The limit of quantification (LOQ) is defined as the lowest concentration that can be quantified reliably with a specified level of accuracy and precision. It is the lowest concentration at which the precision expressed by RSD of less than 2%. LOQ = 10X S.D of y intercept Slope of Calibration Curve In this study the analyte response is 10 times greater than the noise response. For this study six replicates of the analyte at lowest concentration in the calibration range were measured and quantified. The LOD and LOQ 5.28
30 of Amikacin obtained by the proposed method were 20 and 65 µg/ml respectively. (Table-VIII.5.1) Table-VIII.5.2 LOD and LOQ of Amikacin Parameter Value (µg/ml) LOD 20 LOQ 65 System Precision and System Suitability: System precision and system suitability studies were carried out by injecting six replicates of the working standard solution. The % RSD for the peak areas obtained was calculated. The data presented in Table VIII.5.2 reveals that %RSD is <1 and establishes reproducible performance of the instrument. The system suitability parameters are presented in Table-IX.5.2. Injection Number Table- IX.5.2. System Precision Peak Area Theoretical Plates Mean SD %RSD
31 Estimation of the drug from dosage forms. In view of the satisfactory results obtained with the method development for the assay of Amikacin, the author has attempted its applicability for the estimation of the drug in its formulations. Ten tablets of Amikacin were weighed and powered into uniform size in a mortar. An average weight of a tablet was calculated from this powder. An accurately weighed portion from this powder equivalent to 100mg of Amikacin was transferred to 100mL volumetric flask containing 20 ml of mobile phase. The contents of the flask were sonicated for about 20 min. for complete solubility of the drug and the volume was made up to 100 ml with water. Then the mixture was filtered through 0.45µ membrane filter 4mL of above solution was taken into a separate 100 ml volumetric flask and made up to the volume with mobile phase and mixed well. The above solution (20µL) was then injected six times into the column. The mean peak area of the drug was calculated and the drug content in the formulation was calculated by the regression equation of the method. The results of the recovery are tabulated. The percent recovery was reported in Table-X.5.2. Table-X.5.2.Analysis of formulations & Recovery experiments Sample Labeled Amount Amount found %Recovery AMISTAR 100mg AMIKEF 100 mg
32 RESULTS AND DISCUSSION The present study was a humble presentation of the author in developing a sensitive, precise and accurate HPLC method for the analysis of Amikacin in bulk drug and pharmaceutical dosage forms. In order to affect analysis of the component peaks, mixtures of acetonitrile with phosphate buffer in different combinations were tested as mobile phase on a C 18 stationary phase. A mixture of acetonitrile, methyl alcohol, and o- phosphoric acid,opa, (0.1%) in 50:35:15 (v/v) was proved to be the most suitable of all combinations since the chromatographic peaks were better defined and resolved and almost free from tailing. The retention time obtained for Amikacin was 12 min. Each of the samples was injected six times and the same retention times were obtained in all cases. The peak areas of Amikacin were reproducible as indicated by low coefficient of variation. A good linear relationship (r = 0.995) was observed between the concentration of Amikacin and the respective peak areas. The regression curve was constructed by linear regression fitting and its mathematical expression was Y= X-0.03 where Y gives peak area and X is the concentration of the drug. The regression characteristics are given in Table II.5.2. When Amikacin solutions containing 40,80,120,160 µg/ml was analyzed by the proposed method for finding out intra and inter day variations, low % RSD 5.31
33 was observed. High recovery values obtained from the dosage form by the proposed method indicates the method is accurate. The absence of additional peaks indicates non interference of common excipients used in the tablets. The drug content in tablets was quantified using the proposed analytical method. The tablets were found to contain an average of 99.65% of the labeled amount of the drug. The deliberate changes in the method have not much affected the peak tailing theoretical plates and percent assay. This indicates that the present method is robust. The lowest values of LOD and LOQ as obtained by the proposed method indicate the method is sensitive. The standard solution of the drug was stable up to 24 hours as the difference in percent assay is within acceptable limit. System suitability parameters were studied with six replicates standard solution of the drug and the calculated parameters are within the acceptance criteria. The tailing factor and the number of theoretical plates are in the acceptable limits. Conclusion. Hence the author concludes that the proposed HPLC method is sensitive and reproducible for the analysis of Amikacin in pharmaceutical dosage forms with short analysis time. 5.32
34 REFERENCES 1. Juan Manuel Serrano and Manuel Silva, Journal of Chromatography A (2006), 1117, # 2, Lorena Baietto, Analytical and Bioanalytical Chemistry,396, #2, Gallon, O; Guillet-Caruba, C.; Lamy, B.; Laurent, F.; Doucet-Populaire, F.; Decousser, European Journal of Clinical Microbiology & Infectious Diseases,( 2009), 28, 10, Baietto L, D'Avolio A, De Rosa FG, Garazzino S, Michelazzo M, Ventimiglia G, Siccardi M, Simiele M, Sciandra M, Di Perri G. Anal Bioanal Chem.( 2010),396(2): E.pub (2009) Nov Yokoyama yoko HPLC Annals of Gunma University School of Health Sciences,. (2005) 25; Grazyna Ginalska, Dorota Kowalczuk,Monika Osińska International Journal of Pharmaceutics (2007); 339(1-2): Sa Nicoli; Patrizia Santi (pp.). Journal of Pharmaceutical and Biomedical Analysis.41, #3, Gordana Brajansoki J. Chromatogr. B, (2008), Christopher Crafts, Marc Plante, Ian Acworth, Paul Gamache, John Waraska and Bruce Corona ultra Detection,Doinex online 5.33
35 10. A. Papp Catherine A. Knupp a and Rashmi H. Barbhaiya Journal of Chromatography B: Biomedical Sciences and Applications, (1992) 574, # 1, Burkhard Wichert, Hans Schreier and Hartmut Derendorf Journal of Pharmaceutical and BiomedicalAnalysis, (1991)Volume 9, Issue 3, Juan Manuel Serrano a and Manuel Silva Journal of Chromatography B, (2006), 843, # 1, C. H. Feng, S. J. Lin, H. L. Wu and S. H. Chen Chemistry and Materials Science Chromatographia 53, Supplement
Chapter-4 EXPERIMENTAL WORK BY RP-HPLC
Chapter-4 EXPERIMENTAL WORK BY RP-HPLC 4.0 EXPERIMENTAL WORK BY RP-HPLC 4.1. DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR THE DETERMINATION OF NIFLUMIC ACID 4.1.1. MATERIALS AND METHODS OF NIFLUMIC
More informationValidated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form
International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.3, pp 1228-1232, July-Sept 2012 Validated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form Manzoor
More informationStability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage form
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Stability indicating RP-HPLC method for determination of azilsartan medoxomil in bulk and its dosage form
More informationInternational Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010
International Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010 RP HPLC ESTIMATION OF EZETIMIBE IN TABLET DOSAGE FORMS NAGARAJU. P *, KRISHNACHAITHANYA. K, CHANDRABABU. D, SRINIVAS.
More informationAnalytical method development and validation of carvedilol in bulk and tablet dosage form by using uv spectroscopic method as per ich guidelines
IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: ISSN:2320-2831 Research article Open Access Analytical method development and validation of carvedilol in bulk and tablet dosage form by using
More informationAnalytical method development and validation of gabapentin in bulk and tablet dosage form by using UV spectroscopic method
IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: www.ijpar.com ISSN:2320-2831 Research article Open Access Analytical method development and validation of gabapentin in bulk and tablet dosage
More informationImpact factor: 3.958/ICV: 4.10 ISSN:
Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 99 Pharma Science Monitor 9(4), Oct-Dec 2018 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com
More informationVolume 6, Issue 2, January February 2011; Article-015
Research Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE DETERMINATION OF DAPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION USING AN EXPERIMENTAL DESIGN Pratik Mehta*, Ujjwal Sahoo,
More information7. Stability indicating analytical method development and validation of Ramipril and Amlodipine in capsule dosage form by HPLC.
7. Stability indicating analytical method development and validation of and in capsule dosage form by HPLC. 7.1 INSTRUMENTS AND MATERIALS USED 7.1.1 INSTRUMENTS 1. Shimadzu LC-2010 CHT with liquid chromatograph
More informationRP-HPLC Method Development and Validation of Dapagliflozin in Bulk and Tablet formulation
221 IJPAR Volume 2 Issue 4 Oct - Dec -2013 ISSN: 2320-2831 Available Online at: [Research article] RP-HPLC Method Development and Validation of Dapagliflozin in Bulk and Tablet formulation Jeyabaskaran.M
More informationA RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF PARA- PHENYLENEDIAMINE IN PURE FORM AND IN MARKETED PRODUCTS
A RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF PARA- PHENYLENEDIAMINE IN PURE FORM AND IN MARKETED PRODUCTS CH.MOUNIKA*, M.KINNERA Research Article SIR.C.R.REDDY COLLEGE OF PHARMACEUTICAL SCIENCES, ELURU.
More informationDevelopment And Validation Of Rp-Hplc Method For Determination Of Velpatasvir In Bulk
International Journal of Engineering Science Invention (IJESI) ISSN (Online): 2319 6734, ISSN (Print): 2319 6726 www.ijesi.org PP. 36-41 Development And Validation Of Rp-Hplc Method For Determination Of
More informationReceived: ; Accepted:
International Journal of Institutional Pharmacy and Life Sciences 2(1): January-February 2012 INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES Pharmaceutical Sciences Research Article!!!
More informationReverse Phase High Performance Liquid Chromatography method for determination of Lercanidipine hydrochloride in bulk and tablet dosage form
Research Article ISSN: 0974-6943 M.V.Kumudhavalli et al. / Journal of Pharmacy Research 2014,8(11), Available online through http://jprsolutions.info Reverse Phase High Performance Liquid Chromatography
More informationZero And First Order Derivative Spectrophotometric Methods For Determination Of Dronedarone In Pharmaceutical Formulation
International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.1, pp 217-221, Jan-Mar 2013 Zero And First rder Derivative Spectrophotometric Methods For Determination f Dronedarone
More informationMETHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS
CHAPTER 6 METHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS School of Pharmaceutical Sciences, Vels University 106 METHOD DEVELOPMENT AND VALIDATION
More informationNew Spectrophotometric Multicomponent Estimation of Ciprofloxacin and Tinidazole Tablets
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.5, No.1, pp 42-46, Jan-Mar 2013 New Spectrophotometric Multicomponent Estimation of Ciprofloxacin and Tinidazole Tablets
More informationSPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF MIZOLASTINE IN PHARMACEUTICAL DOSAGE FORMS
Int. J. Chem. Sci.: 8(2), 2010, 1301-1307 SPECTROPHOTOMETRIC METHODS FOR ESTIMATIO OF MIZOLASTIE I PHARMACEUTICAL DOSAGE FORMS A. SREELAKSHMI*, G. DEVALA RAO a and G. SUDHAKARA SAI BABU a Department of
More informationCHAPTER - 3 ANALYTICAL PROFILE. 3.1 Estimation of Drug in Pharmaceutical Formulation Estimation of Drugs
CHAPTER - 3 ANALYTICAL PROFILE 3.1 Estimation of Drug in Pharmaceutical Formulation 3.1.1 Estimation of Drugs ANALYTICAL PROFILE 84 3.1 ESTIMATION OF DRUG IN PHARMACEUTICAL FORMULATION. Agrawal A et al
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(1): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(1):118-122 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of High Performance Liquid
More informationValidated First Order Derivative Spectroscopic Method for the determination of Stavudine in Bulk and Pharmaceutical Dosage Forms
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.3, No.1, pp 18-22, Jan-Mar 2011 Validated First Order Derivative Spectroscopic Method for the determination of Stavudine
More informationAppendix II- Bioanalytical Method Development and Validation
A2. Bioanalytical method development 1. Optimization of chromatographic conditions Method development and optimization of chromatographic parameters is of utmost important for validating a method in biological
More informationInternational Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: , ISSN(Online): Vol.9, No.7, pp , 2016
International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 097-30, ISSN(Online): 255-9563 Vol.9, No.7, pp 399-06, 2016 Analytical Quality by Design Approach for Development of UV-Spectrophotometric
More informationNEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF OXOLAMINE IN FORMULATION
NEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF OXOLAMINE IN FORMULATION V.PhaniKumar 1 *, CH.Venkata Kishore 2 1 Department of Chemistry, Govt college, Tiruvuru, Krishna District Andhra
More informationDevelopment and validation a RP-HPLC method: Application for the quantitative determination of quetiapine fumarate from marketed bulk tablets
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(1):142-146 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and validation a RP-HPLC method: Application
More informationDepartment of Quality Assurance, Luqman College of Pharmacy, GULBARGA (K.S.) INDIA ABSTRACT
Int. J. Chem. Sci.: 12(3), 2014, 871-879 ISSN 0972-768X www.sadgurupublications.com DEVELPMENT AND VALIDATIN F A RAPID RP HPLC METHD FR THE DETERMINATIN F CINITAPRIDE HYDRGEN TARTARATE IN PURE AND ITS
More informationValidation of Stability-Indicating RP-HPLC Method for the Assay of Ibrutinib in Pharmaceutical Dosage form
Validation of Stability-Indicating RP-HPLC Method for the Assay of Ibrutinib in Pharmaceutical Dosage form 8.1 Introduction Ibrutinib (IBR) is an anticancer drug targeting B-cell malignancies (blood cancer
More informationA Simple, Sensitive Spectrophotometric Determination of Mosapride in Pharmaceutical Preparations Using Novel Reagent
ISS: 0973-4945; CODE ECJHAO E- Chemistry http://www.e-journal.net Vol. 1, o. 5, pp 267-271, October 2004 A Simple, Sensitive Spectrophotometric Determination of Mosapride in Pharmaceutical Preparations
More informationA Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation
Pharmaceutical Research A Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation Manisha Puranik* a, Sailesh Wadher b and Kritika
More informationCHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC. Diclofenac (gift sample from M/s Micro Labs Ltd., Pondicherry)
CHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC A UV spectrophotometric method based on the measurement of absorbance at 276nm in phosphate buffer of p H 7.4 was used in the present study of the
More informationSTABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG AND IT S PHARMACEUTICAL DOSAGE FORM
International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 3, Issue 3, 2011 Research Article STABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG
More informationDevelopment of Validated Analytical Method of Mefenamic Acid in an Emulgel (Topical Formulation)
Development of Validated Analytical Method of Mefenamic Acid in an Emulgel (Topical Formulation) TEENA OSWAL*, DR.SURYAKANT BHOSALE, DR. SONALI NAIK MET Institute Of Pharmacy Met Complex, Bandra Reclamation,
More informationSTABILITY INDICATING METHOD OF RELATED IMPURITIES IN VENLAFAXINE HYDROCHLORIDE SUSTAINED RELEASE TABLETS
Issn No: 976-39 RESEARCH ARTICLE STABILITY INDICATING METHOD OF RELATED IMPURITIES IN VENLAFAXINE HYDROCHLORIDE SUSTAINED RELEASE TABLETS CHETLAPALLI SATYA SRINIVAS 1, P.RENUKA DEVI 2 and GAMPA VIJAYAKUMAR*
More informationDEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION
Page186 Research Article Pharmaceutical Sciences DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION Kishore Konam 1 &
More informationInternational Journal of Pharma and Bio Sciences V1(1)2010 UV- SPECTROPHOTOMETRIC DETERMINATION OF TENATOPRAZOLE FROM ITS BULK AND TABLETS
M. SUGUMARAN*, R.NAGESWARA RAO AND D. JOTHIESWARI Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur- 603319,Tamilnadu,India. * Corresponding author murugesansugumaran@yahoo.com
More informationRP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.ejchem.net 2012, 9(3), 1407-1411 RP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms M. VIJAYA LAKSHMI 1, J.V.L.N.S. RAO 2 AND A. LAKSHMANA
More informationDETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
CHAPTER 9 DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 9 Determination of drug release during
More informationChapter 4: Verification of compendial methods
Chapter 4: Verification of compendial methods Introduction In order to ensure accurate and reliable test results, the quality control laboratory (QCL) needs to use analytical methods (and accompanying
More informationResearch Article. Figure 1. Chemical structure of doxofylline. Indonesian J. Pharm. Vol. 24 No. 1 : ISSN-p :
Research Article Indonesian J. Pharm. Vol. 24 No. 1 : 14 21 ISSN-p : 0126-1037 DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL
More informationInt. J. Pharm. Sci. Rev. Res., 30(2), January February 2015; Article No. 09, Pages: 63-68
Research Article Stability indicating RP-HPLC Method for Determination of FexoFenadine Hydrochloride and its Related Substances in Active Pharmaceutical Substance Abhay Gupta* 1, Dr. Birendra Srivastava,
More informationInternational Journal of Pharma and Bio Sciences
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACEUTICAL ANALYSIS DEVELOPMENT, ESTIMATION AND VALIDATION OF BOSENTAN IN BULK AND IN ITS PHARMACEUTICAL FORMULATION BY UV-VIS SPECTROSCOPIC
More informationResearch Article Available online at
Research Article Available online at www.jgtps.com ISSN: 2230-7346 Volume- 5, Issue -2, pp-1522-1527- April June (2014) DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CITICOLINE
More informationSIMPLE AND SENSITIVE VALIDATED REVERSE PHASE HPLC-UV METHOD FOR THE DETERMINATION OF LYMECYCLINE IN PHARMACEUTICAL DOSAGE FORMS
IJPSR (2012), Vol. 3, Issue 07 (Research Article) Received on 26 March, 2012; received in revised form 25 June, 2012; accepted 28 June, 2012 SIMPLE AND SENSITIVE VALIDATED REVERSE PHASE HPLC-UV METHOD
More informationResearch Article. UV Spectrophotometric Estimation of Alprazolam by second and third order derivative Methods in Bulk and Pharmaceutical Dosage Form
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(4):272-278 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 UV Spectrophotometric Estimation of Alprazolam by
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF GABAPENTIN IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 10(4), 2012, 2209-2217 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF GABAPENTIN IN PURE AND PHARMACEUTICAL FORMULATIONS
More informationAsian Journal of Research in Chemistry and Pharmaceutical Sciences Journal home page:
Research Article ISSN: 2349 7106 Asian Journal of Research in Chemistry and Pharmaceutical Sciences Journal home page: www.ajrcps.com ESTIMATION OF RAMELTEON IN TABLET DOSAGE FORM BY HPLC M. Jyothsna*
More informationMethod development and validation for the estimation of metronidazole in tablet dosage form by UV spectroscopy and derivative spectroscopy
IJPAR Volume 3 Issue 2 April-June-2014 ISSN: 2320-2831 Available Online at: www.ijpar.com [Research article] Method development and validation for the estimation of metronidazole in tablet dosage form
More informationJournal of Pharmaceutical and Biomedical Analysis Letters. Analysis Letters
Naga Jyothi. C et al, JPBMAL, 2015, 3(1): 242 246 ISSN: 2347-4742 Journal of Pharmaceutical and Biomedical Analysis Letters Journal Home Page: www.pharmaresearchlibrary.com/jpbmal Research Article Open
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD TO DETERMINE CINITAPRIDE HYDROGEN TARTARATE IN BULK AND PHARMACEUTICAL FORMULATION
Research Article ISSN:2230-7346 Journal of Global Trends in Pharmaceutical Sciences Vol.3, Issue 2, pp -619-627, April June 2012 DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD TO DETERMINE CINITAPRIDE HYDROGEN
More informationPelagia Research Library. Spectrophotometric determination of Ametoctradin and in its commercial formulations
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2016, 7(4):7-14 ISSN: 0976-8688 CODEN (USA): PSHIBD Spectrophotometric determination of Ametoctradin and in its commercial formulations
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
75 CHAPTER 3 DEVELOPMENT AND APPLICATION OF STABILITY-INDICATING HPLC METHOD FOR THE DETERMINATION OF NEVIRAPINE AND ITS IMPURITIES IN COMBINATION DRUG PRODUCT 3.1 INTRODUCTION OF DOSAGE FORM AND LITERATURE
More informationDissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form
Research Journal of Recent Sciences ISSN 2277-252 Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form Abstract Rele Rajan
More informationJournal of Chemical and Pharmaceutical Research, 2012, 4(6): Research Article. Estimation of zaleplon by a new RP-HPLC method
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(6):3010-3014 Research Article ISS : 0975-7384 CODE(USA) : JCPRC5 Estimation of zaleplon by a new RP-HPLC method Tentu.
More informationINTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND LIFE SCIENCES
A. Chenthilnathan et al IJRPLS, 2014, 2(2): 185-190 Research Article Available online at www.pharmaresearchlibrary.com/ijrpls ISSN: 2321-5038 INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND LIFE SCIENCES
More informationDEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR DETERMINATION OF LOPINAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR DETERMINATION OF LOPINAVIR IN BULK AND
More informationSensitive Spectrophotometric Method for the Determination of Prazosin
Available online at www.ijacskros.com Sensitive Spectrophotometric Method for the Determination of Prazosin G. Dilli Rani 1, C. Narasimha Rao 1, C. Narasimha Rao 2, A. Narayana 3, P. Venkateswarlu 1 *
More informationINTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES
International Journal of Institutional Pharmacy and Life Sciences 4(2): March-April 2014 INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES Pharmaceutical Sciences Original Article!!! Received:
More informationDevelopment and Validation of Stability Indicating Assay Method of Etodolac by using UV-Visible Spectrophotometer
Research Article Development and Validation of Stability Indicating Assay Method of Etodolac by using UV-Visible Spectrophotometer Aniruddha J Palande, Shailaja B Jadhav *, Amit S Tapkir, Pravin D Chaudhari,
More informationDevelopment and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage form
IJPAR Vol.5 Issue 4 Oct - Dec -2016 Journal Home page: ISSN:2320-2831 Research article Open Access Development and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage
More informationSimultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(4), 1620-1625 Simultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium DESHMUKH HAFSA, S. CHANDA
More informationValidated spectrophotometric determination of Fenofibrate in formulation
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2010, 1 (1): 173-178 Validated spectrophotometric determination of Fenofibrate in formulation Krishna R. Gupta*, Sonali S. Askarkar,
More informationDEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR THE DETERMINATION OF AFLOQUALONE IN HUMAN PLASMA AND FORMULATION
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR THE DETERMINATION OF AFLOQUALONE IN HUMAN
More informationSimultaneous Estimation of Metolazone and Spironolactone in Combined Tablet Dosage Form BY UV Spectroscopy.
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.3, No.4, pp 2068-2074, Oct-Dec 2011 Simultaneous Estimation of Metolazone and Spironolactone in Combined Tablet Dosage
More informationNew Simple UV Spectrophotometric Method for Determination of Mirtazapine in Bulk and pharmaceutical dosage forms
New Simple UV Spectrophotometric Method for Determination of Mirtazapine in Bulk and pharmaceutical dosage forms Sk. Benajeer Department of pharmaceutical chemistry, benajeershaik@gmail.com K. Venkata
More informationof nm throughout the experimental work.
Difference Spectrophotometric Methods for Pioglitazone Hydrochloride and Metformin Hydrochloride K.Sujana, K.Abbulu, O.Bala Souri, B.Archana, M.Sindu, G.Swathi Rani Department of Pharmaceutical Analysis,
More informationCu-Creatinine- Metol system
Quantification of Creatinine in Human Serum using Metol as a Chromogenic Probe Materials and methods 6.1. Reagents 6.1.1. N-methyl-p-aminophenol sulfate N-methyl-p-aminophenol sulfate also denoted as Metol
More informationPharmacophore 2011, Vol. 2 (4), ISSN Pharmacophore. (An International Research Journal)
Pharmacophore 2011, Vol. 2 (4), 232-238 ISSN 2229 5402 Pharmacophore (An International Research Journal) Available online at http://www.pharmacophorejournal.com/ Original Research Paper SIMULTANEOUS ANALYSIS
More informationResearch Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC
ISSN 2395-3411 Available online at www.ijpacr.com 248 Research Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC Mantha Vebkatesh* and A. Yasodha
More informationMethod Development and Validation Of Prasugrel Tablets By RP- HPLC
Method Development and Validation Of Prasugrel Tablets By RP- HPLC K.Sonia*, Ndwabe Hamunyare, K.Manikandan Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur,
More informationJ Pharm Sci Bioscientific Res (4): ISSN NO
Development and Validation of Stability Indicating Analytical Method for Simultaneous Estimation of Perindopril and Potassium in Their Combined Marketed Dosage Form ABSTRACT: Gurjeet Kaur*, Nikhil Patel
More informationKEYWORDS: Acetaminophen, Doxylamine succinate, Dextromethorphan hydrobromide.
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 6718, ISSN (Print): 2319 670X Volume 3 Issue 7 July 2014 PP.08-12 Analytical method development and validation of acetaminophen,
More informationINTERNATIONAL RESEARCH JOURNAL OF PHARMACY
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 8407 Research Article STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF CEFIXIME
More informationSpectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form
Spectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form Kailash P Prajapati *, A Bhandari INDO GLOBAL JOURNAL OF PHARMACEUTICAL SCIENCES ISSN 2249-1023 Faculty of Pharmaceutical
More informationA Reverse Phase Liquid Chromatography Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.3, pp 1136-1141, July-Sept 2012 A Reverse Phase Liquid Chromatography Analysis of Citicoline Sodium in Pharmaceutical
More informationSIMULTANEOUS RP HPLC DETERMINATION OF CAMYLOFIN DIHYDROCHLORIDE AND PARACETAMOL IN PHARMACEUTICAL PREPARATIONS.
Ind. J. Anal. Chem Vol. 7 11. 2008 SIMULTANEOUS RP HPLC DETERMINATION OF CAMYLOFIN DIHYDROCHLORIDE AND PARACETAMOL IN PHARMACEUTICAL PREPARATIONS. Authors for correspondence : R. R. Singh1*, M. V. Rathnam,
More informationANALYSIS OF AMISULPRIDE IN PHARMACEUTICAL DOSAGE FORMS BY NOVAL SPECTROPHOTOMETRIC METHODS
Int. J. Chem. Sci.: 10(1), 2012, 203-212 ISSN 0972-768X www.sadgurupublications.com ANALYSIS OF AMISULPRIDE IN PHARMACEUTICAL DOSAGE FORMS BY NOVAL SPECTROPHOTOMETRIC METHODS P. RAVI SANKAR *, CH. DEVADASU
More informationINTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE
INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE DEVELOPMENT OF VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF BETAXOLOL HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM SIDHARTH
More informationIJPRD, 2012; Vol 4(10): December-2012 ( ) International Standard Serial Number
IJPRD, 212; Vol 4(1): December-212 (85 92) International Standard Serial Number 974 9446 --------------------------------------------------------------------------------------------------------------------------------------------------
More informationDevelopment And Validation Of Stability Indicating RP-HPLC Method For Estimation Of Ledipasvir And Sofosbuvir
International Journal of Engineering Science Invention (IJESI) ISSN (Online): 2319 6734, ISSN (Print): 2319 6726 www.ijesi.org PP. 42-48 Development And Validation Of Stability Indicating RP-HPLC Method
More informationAnalytical Method Development and Validation of Lafutidine in Tablet dosage form by RP-HPLC
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3, No.3, pp 1403-1407, July-Sept 2011 Analytical Method Development and Validation of Lafutidine in Tablet dosage form
More informationAREA UNDER CURVE AND SECOND ORDER DERIVATIVE SPECTROSCOPY OF METAXALONE IN BULK DRUG AND TABLET FORMULATION
Int. J. Chem. Sci.: 8(2), 2010, 823-827 AREA UNDER CURVE AND SECND RDER DERIVATIVE SPECTRSCPY F METAXALNE IN BULK DRUG AND TABLET FRMULATIN J. PRIYADHARISINI, G. P. GIGI, V. NIRAIMATHI and A. JERAD SURESH
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com HPLC AND UV-SPECTROPHOTOMETRIC ESTIMATION OF TENELIGLIPTIN
More informationNovus International Journal of Analytical Innovations 2012, Vol. 1, No. 3
Novus International Journal of Analytical Innovations 2012, Vol. 1, No. 3 ISSN 2278-6953 www.novusscientia.org Accepted on October 22, 2012 RP-HPLC method for simultaneous estimation of Avitriptan and
More informationPharmacophore 2014, Vol. 5 (2), USA CODEN: PHARM7 ISSN Pharmacophore. (An International Research Journal)
Pharmacophore 2014, Vol. 5 (2), 252-257 USA CODEN: PHARM7 ISSN 2229-5402 Pharmacophore (An International Research Journal) Available online at http://www.pharmacophorejournal.com/ Original Research Paper
More informationInternational Journal of Current Trends in Pharmaceutical Research. International Journal of Current Trends in Pharmaceutical Research
International Journal of Current Trends in Pharmaceutical Research Journal Home Page: www.pharmaresearchlibrary.com/ijctpr Research Article Open Access Development and Validation Levofloxacin Andambroxol
More informationDevelopment and validation of UV- spectrophotometric method for the estimation of dabigatran etexilate mesylate (dem)
IJPAR Vol.5 Issue 1 Jan- Mar -2016 Journal Home page: ISSN:2320-2831 Research article Open Access Development and validation of UV- spectrophotometric method for the estimation of dabigatran etexilate
More informationDevelopment and Validation of Stability Indicating RP-HPLC Method for the Determination of Anagrelide HCl in Pharmaceutical Formulation
ISSN 0976 3333 Available Online at www.ijpba.info International Journal of Pharmaceutical & Biological Archives 2013; 4(2): 342-346 ORIGINAL RESEARCH ARTICLE Development and Validation of Stability Indicating
More informationSimultaneous estimation and validation of bromhexine and cephalexin in bulk and pharmaceutical dosage form by RP-HPLC method
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2014, 6 (6):315-321 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationSimultaneous estimation of amitryptyline and chlordiazepoxide by RP-HPLC method
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Simultaneous estimation of amitryptyline and chlordiazepoxide by RP-HPLC method Dr.R.Srinivasan*, K.Lurdhu
More informationInternational Journal of Pharma and Bio Sciences V1(1)2010. HPLC method for analysis of Lercanidipine Hydrochloride in Tablets
G. MUBEEN,, MAMTA PAL, AND M.N. VIMALA* Department of, Al-Ameen College of Pharmacy, Bangalore, India. * Corresponding author vimalamn_325@yahoo.co.in ABSTRACT A reverse phase HPLC method was developed
More informationResearch Article. Simultaneous spectrophotometric estimation of Paracetamol and Aceclofenac by second order derivative method in combined dosage form
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(6):512-517 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Simultaneous spectrophotometric estimation of Paracetamol
More informationNEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF PARACETAMOL IN PURE FORM AND PHARMACEUTICAL FORMULATIONS
NEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF PARACETAMOL IN PURE FORM AND PHARMACEUTICAL FORMULATIONS Siva lokesh.b, Uma maheswari.t, Anusha.B, Ramya.B, Sri Sai Rohini.T, Sudheerbabu.I* Sir
More informationInternational Journal of
International Journal of Chemical, Environmental and Pharmaceutical Research Vol. 5, No.1, 13-18 January - April, 2014 Indirect Visible Spectrophotometric Method For the Determination of Framycetin with
More informationSaudi Journal of Medical and Pharmaceutical Sciences
Saudi Journal of Medical and Pharmaceutical Sciences Scholars Middle East Publishers Dubai, United Arab Emirates Website: http://scholarsmepub.com/ ISSN 2413-4929 (Print) ISSN 2413-4910 (Online) Development
More informationResearch Article Spectrophotometric Estimation of Didanosine in Bulk Drug and its Formulation
Research Article Spectrophotometric Estimation of Didanosine in Bulk Drug and its Formulation RN. Kane, PS. Bhokare*, CC. Nalawade, MS Sayyed and RD. Paliwal Department of Pharmaceutical Chemistry, Singhad
More informationInternational Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:
Research Article CODEN: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION
More informationDepartment of Chemistry, JNTUACE, Kalikiri
Method Development and Validation of Aegle marmeleous M. Swetha 1*, N. Saritha 1, N. Devanna 2 1 Department of Chemistry, JNTUACE, Kalikiri.-517234 2 Department of Chemistry, JNTUA, Anthapuramu -515002
More informationDevelopment and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method
21 Article Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method Mali Audumbar Digambar*, Jadhav Santosh, Mane Pandurang,
More informationUV spectrophotometric determination of pimozide in bulk and tablet dosage forms
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Short
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2010, 2(5):399-417 Method develpopment and validation of Hydrochloride
More information