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1 SPM ( ) NANOBRICK CO., Ltd o8. 12
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3 Overview Management Profile Established : May 2007 Capital : 3 Million USD Employees : 45 (PhD 4, MD 12) Location : Head Office (Suwon), Marketing (Seoul), Factory / R&D Center (Pyeongtaek) Product : Fine Chemical- Nano Materials Electrically Controllable Material Magnetically Controllable Material * 2012 TOKYO NANOTECH Grand Prize * 2012 NANO KOREA Grand Prize * 2013 G-STAR Enterprise selected Intellectual Property Patent Applied 160 / Registered 41 Trademark Applied 28 건 / Registered 14 * Selected as an IP Star Enterprise * IP R&D Business with Korean IP Office CEO CFO CTO CPO CMO IP Advisor PhD at Seoul National Univ.(Electrical Material) UC Berkeley, Special Researcher Samsung Electronics(Planning/Investment) MD at Seoul National Univ.(Business Manag.) Certified Public Accountant Investment Corp. Law Firm > 10 years Carnegie Mellon Univ. (Chemistry) Government R&D Center > 15 years Production & Quality Control Specialist Related Background > 20 years Kyungbuk National Univ.(Electronic Engineering) Marketing, Sales, PR Specialist(Himart) Related Background > 25 years Patent attorney, Dr of Engineering IP Office CEO, PA at Kim& Chang
4 The Pioneer of Nano-technology Based Performance Materials
5 Global IP STAR Company Patent Trademarks Applied Registered Applied Registered
6 What s SPM? Superparamagnetic nanoparticles are Nanomagnetic particles which include the property of superparamagnetism : a type of magnetism that occurs in small ferromagnetic or ferrimagnetic nanoparticles. SPM is composed of para-magnetic nanoparticles that are coated with various Its superior properties attributed to high magnetization, stability to water with minimize aggregation, mono-dispersed size. They allow accept cross linked molecules such as nucleic acids, proteins, antibodies and fluorescent proteins from cell lysates. Product Benefits Uniformly dispersed Particle High magnetization Value High concentrated functional group coating Cost competitive, qualify ensured Easy and efficient separation of Biomolecule
7 PRINCIPLE PRODUCT TYPE Product Characteristics Properties & Benefits Application SPM Non-coated surface Particle size : 200nm~3um Magnetization : ~80emu/g Uniform particle size High magnetization value High dispersion Coated with various ligands, and quickly and easily remove the material SPM-OH Surface hydroxyl groups Particle size : 200nm ~ 3um Negative surface charge at ph 7.0 Fast procedure High reproducibility Isolation and purification of nucleic acids from biological samples SPM-NH2 SPM-COOH Surface amine groups Particle size : 200nm ~ 3um Pasitive surface charge at ph 7.0 Surface carboxyl groups Particle size : 200nm ~ 3um Negative surface charge at ph 7.0 Direct covalent binding through reductive aminated aldehydes Rapid binding at neutral to High ph and room temperature Amide bond formation with primary amine Immediate peptide bond formation at ph 5~6 and room temperature Isolation of cell, protein, or other target molecules Immobilization of labeled protein and peptides in vitro diagnosis
8 SEM image, XRD, Hysteresis analysis Magnetite based SPM exert excellent separation property with its monodispersed particle and sufficient magnetization up to 80emu/g.
9 SPM-OH (160nm) SPM-NH2 (160nm) SPM-COOH (160nm)
10 SPM nanoparticle Technical specification - Average Particle Size: 200nm, 300nm, 400nm, 1um, 3um (SEM) - Particle Concentration: 3 wt% in H2O - Surface electric charge : Positive (SPM-NH2) Negative (SPM-OH, SPM-COOH) - Zeta potential : > - 40mV [SEM image] [SPM-500 Zeta potential]
11 Compared SPM with Competent company A company B company Nanobrick SPM Data indicate that SPM has high magnetization property and sharp peak than comparable counterpart products
12 Separation of DNA/RNA Antibody detection MRI imaging agent Biosensor Bio remediation Drug Delivery system Water treatment Protein purification
13 1. Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticlebased extraction and an enzyme-linked immunosorbent assay. Lee HM 1, Song SO, Cha SH, Wee SB, Bischoff K, Park SW, Son SW, Kang HG, Cho MH. J Vet Sci. 2013;14(2): Epub 2013 Feb 5. - The conjugation of antibody to SPM-NH2: amount of used ZEN Antibody μg Product Other companies SPM(company C ) Nanobrick SPM Binding efficiency ± 15% ± 5% - Application ZEN toxin(antigen) to SPM + ZEN Antibody coupling solution - Using SPM-Zen toxin(antigen) + ZEN(antibody) solution 1ml - Measured by using HPLC equipment Product Antigen Concentration (ppb) Recovered Antigen concentration(ppb) through SPM + ZEN Antibody Binding(%) Other companies SPM-1 (company C) Other companies SPM-2 (company C) % % Nanobrick SPM % Nanobrick SPM % College of Veterinary medicine,cornell University, NY, USA College of Veterinary medine at Seoul National University, Korea Animal and Plant Quarantine Agency, Korea,e of Veterinary Medicine
14 2. A Solid State Nanopore Device for Investigating the Magnetic Properties of Magnetic Nanoparticles SangYoon Park, Jaekwan Lim, Y. Eugene Pak, Seunghyun Moon, and Yoon-Kyu Song Sensors (Basel) June; 13(6): Advanced Institute of Convergence Technology,Seoul National University, Korea
15 3. Size-dependent magnetophoresis of native single super-paramagneticnanoparticles in a microchip Peng Zhang, Sangyoon Park and Seong Ho Kang Chem. Commun., 2013,49, Department of Advanced Chemistry, Hyung hee University, Korea Advanced Institute of Convergence technology,seoul National University,Korea
16 4. DNA Purification Gel electrophoresis 그래프와중복되는수치. 생략하고대체데이터필요. 추가필요한자료 1.SDS-PAGE Image 2.BSA 혹은 Streptavidin 에대한실험데이터 3.Coating 제품에대한실험자료 4.Protein A, Antibody binding affinity 확인필요 5. Future prospective 향후진출계획부분필요 6. Separator 장비관련시제품데이터 Using SPM from Nanobrick, Target DNA was efficiently purified compared to commercial magnetic bead or Agarose Bead. BioOne Co., Ltd
17 Thank you
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